AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59654

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59654.t1 MTR_5g054300 94.215 363 19 2 1 363 1 361 0.0 686
MS.gene59654.t1 MTR_1g093080 59.599 349 111 13 10 333 1 344 2.46e-123 360
MS.gene59654.t1 MTR_1g093080 58.935 263 83 8 10 249 1 261 1.64e-88 268
MS.gene59654.t1 MTR_1g093080 55.696 237 80 8 10 223 1 235 9.22e-71 222
MS.gene59654.t1 MTR_4g113140 36.915 363 154 15 4 310 7 350 9.77e-52 178
MS.gene59654.t1 MTR_4g086835 34.783 345 144 13 16 296 41 368 9.43e-43 154
MS.gene59654.t1 MTR_4g086835 34.783 345 143 14 16 296 41 367 7.19e-41 149
MS.gene59654.t1 MTR_5g017980 38.492 252 106 8 19 260 42 254 1.64e-36 135
MS.gene59654.t1 MTR_5g017980 38.492 252 104 9 19 260 42 252 1.47e-34 129
MS.gene59654.t1 MTR_5g017980 38.492 252 105 9 19 260 42 253 1.67e-34 129
MS.gene59654.t1 MTR_7g098250 45.783 83 45 0 175 257 238 320 9.87e-14 72.4
MS.gene59654.t1 MTR_5g014040 49.333 75 34 3 193 266 304 375 1.06e-13 72.8
MS.gene59654.t1 MTR_1g080330 58.929 56 23 0 197 252 173 228 2.19e-13 70.1
MS.gene59654.t1 MTR_7g115530 58.929 56 23 0 197 252 216 271 3.92e-13 69.7
MS.gene59654.t1 MTR_1g080330 58.929 56 23 0 197 252 173 228 4.43e-13 70.1
MS.gene59654.t1 MTR_1g032570 45.333 75 40 1 193 267 194 267 4.63e-13 70.9
MS.gene59654.t1 MTR_1g032570 45.333 75 40 1 193 267 125 198 4.66e-13 70.5
MS.gene59654.t1 MTR_2g027860 58.182 55 23 0 199 253 49 103 4.80e-13 69.7
MS.gene59654.t1 MTR_4g098870 43.158 95 42 3 173 256 293 386 5.68e-13 70.5
MS.gene59654.t1 MTR_4g121020 41.053 95 43 3 198 290 214 297 5.69e-13 70.5
MS.gene59654.t1 MTR_4g098870 43.158 95 42 3 173 256 273 366 5.75e-13 70.5
MS.gene59654.t1 MTR_7g089010 44.156 77 42 1 181 257 73 148 5.82e-13 68.9
MS.gene59654.t1 MTR_4g121020 41.053 95 43 3 198 290 214 297 5.84e-13 70.5
MS.gene59654.t1 MTR_4g098870 43.158 95 42 3 173 256 273 366 5.98e-13 70.5
MS.gene59654.t1 MTR_4g098870 43.158 95 42 3 173 256 293 386 6.07e-13 70.5
MS.gene59654.t1 MTR_4g098870 43.158 95 42 3 173 256 312 405 6.43e-13 70.1
MS.gene59654.t1 MTR_3g106220 33.333 120 75 2 192 307 191 309 6.62e-13 70.5
MS.gene59654.t1 MTR_4g121020 39.394 99 47 3 198 294 110 197 6.84e-13 70.1
MS.gene59654.t1 MTR_7g115530 58.929 56 23 0 197 252 216 271 8.26e-13 68.9
MS.gene59654.t1 MTR_3g102600 43.038 79 41 2 203 278 87 164 8.33e-13 66.6
MS.gene59654.t1 MTR_7g115530 58.929 56 23 0 197 252 216 271 9.26e-13 68.9
MS.gene59654.t1 MTR_7g088070 55.172 58 26 0 196 253 47 104 1.01e-12 68.2
MS.gene59654.t1 MTR_3g102600 43.038 79 41 2 203 278 136 213 1.04e-12 67.4
MS.gene59654.t1 MTR_7g088070 55.172 58 26 0 196 253 47 104 1.08e-12 68.2
MS.gene59654.t1 MTR_7g115530 58.929 56 23 0 197 252 216 271 1.32e-12 68.9
MS.gene59654.t1 MTR_2g450070 36.207 116 66 4 197 310 211 320 1.50e-12 69.3
MS.gene59654.t1 MTR_2g450070 36.207 116 66 4 197 310 211 320 1.53e-12 69.3
MS.gene59654.t1 MTR_4g021790 46.341 82 35 3 185 258 146 226 1.80e-12 67.4
MS.gene59654.t1 MTR_1g053830 39.474 76 41 1 178 253 2 72 2.41e-12 63.2
MS.gene59654.t1 MTR_2g023580 41.573 89 40 2 164 252 32 108 2.67e-12 66.2
MS.gene59654.t1 MTR_2g034960 28.804 184 120 4 132 310 149 326 2.82e-12 68.2
MS.gene59654.t1 MTR_2g027800 45.205 73 40 0 199 271 39 111 4.05e-12 66.6
MS.gene59654.t1 MTR_7g117705 50.820 61 29 1 196 256 188 247 5.31e-12 67.0
MS.gene59654.t1 MTR_7g115530 58.182 55 23 0 197 251 216 270 6.49e-12 65.9
MS.gene59654.t1 MTR_7g069660 41.304 92 48 2 165 252 35 124 6.77e-12 66.2
MS.gene59654.t1 MTR_1g013170 50.820 61 29 1 197 257 189 248 7.11e-12 67.0
MS.gene59654.t1 MTR_5g041350 54.545 55 25 0 199 253 15 69 8.17e-12 66.2
MS.gene59654.t1 MTR_4g064730 42.105 76 43 1 198 273 130 204 8.56e-12 65.9
MS.gene59654.t1 MTR_5g014040 46.250 80 34 4 193 266 264 340 8.65e-12 66.6
MS.gene59654.t1 MTR_6g444980 58.182 55 23 0 199 253 35 89 9.52e-12 66.2
MS.gene59654.t1 MTR_2g090675 35.922 103 58 2 154 256 123 217 9.76e-12 66.2
MS.gene59654.t1 MTR_5g014040 46.250 80 34 4 193 266 304 380 1.06e-11 66.6
MS.gene59654.t1 MTR_6g444980 58.182 55 23 0 199 253 35 89 1.12e-11 65.9
MS.gene59654.t1 MTR_2g027800 56.364 55 24 0 199 253 39 93 1.53e-11 65.1
MS.gene59654.t1 MTR_1g053835 42.353 85 44 1 169 253 233 312 1.66e-11 65.5
MS.gene59654.t1 MTR_1g112370 44.444 72 33 1 188 252 4 75 2.34e-11 64.7
MS.gene59654.t1 MTR_4g131580 49.180 61 30 1 197 257 198 257 2.80e-11 65.1
MS.gene59654.t1 MTR_7g115530 58.491 53 22 0 197 249 216 268 3.32e-11 63.9
MS.gene59654.t1 MTR_4g081710 52.727 55 26 0 199 253 17 71 5.68e-11 63.5
MS.gene59654.t1 MTR_2g086450 52.727 55 26 0 199 253 45 99 7.30e-11 63.5
MS.gene59654.t1 MTR_4g078810 45.161 62 33 1 195 256 154 214 7.36e-11 63.5
MS.gene59654.t1 MTR_1g090670 54.545 55 25 0 199 253 44 98 8.50e-11 62.8
MS.gene59654.t1 MTR_8g066310 42.045 88 47 2 167 252 141 226 9.40e-11 62.8
MS.gene59654.t1 MTR_6g032990 50.909 55 27 0 199 253 42 96 9.55e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59654.t1 AT1G25550 45.970 335 147 9 1 320 1 316 1.11e-82 255
MS.gene59654.t1 AT1G68670 46.839 348 125 14 1 313 2 324 2.91e-75 237
MS.gene59654.t1 AT1G25550 50.575 261 115 6 1 249 1 259 4.32e-72 226
MS.gene59654.t1 AT3G25790 41.551 361 161 12 19 359 20 350 2.89e-62 203
MS.gene59654.t1 AT1G13300 45.070 284 103 9 20 285 20 268 3.20e-61 200
MS.gene59654.t1 AT3G25790 43.548 248 102 6 19 249 20 246 3.25e-47 161
MS.gene59654.t1 AT2G03500 33.934 333 159 9 1 294 18 328 1.69e-45 161
MS.gene59654.t1 AT4G37180 34.201 269 122 7 11 260 39 271 2.09e-32 124
MS.gene59654.t1 AT4G37180 33.457 269 131 7 11 260 39 278 2.88e-31 122
MS.gene59654.t1 AT4G37180 33.721 258 116 7 11 249 39 260 1.18e-27 110
MS.gene59654.t1 AT1G49560 31.900 279 138 10 16 279 27 268 2.01e-25 105
MS.gene59654.t1 AT1G49560 31.855 248 123 8 16 249 27 242 5.52e-21 91.7
MS.gene59654.t1 AT2G25180 37.600 125 63 4 197 307 193 316 9.87e-14 72.8
MS.gene59654.t1 AT5G58080 51.515 66 30 2 193 257 187 251 1.76e-13 72.0
MS.gene59654.t1 AT5G05090 44.872 78 42 1 180 257 62 138 2.10e-13 70.1
MS.gene59654.t1 AT2G01060 58.182 55 23 0 199 253 15 69 3.21e-13 69.7
MS.gene59654.t1 AT3G10760 51.667 60 28 1 198 257 104 162 1.16e-12 68.6
MS.gene59654.t1 AT2G06020 54.545 55 25 0 199 253 86 140 1.18e-12 68.2
MS.gene59654.t1 AT4G28610 49.254 67 34 0 186 252 212 278 1.25e-12 68.9
MS.gene59654.t1 AT2G40970 51.667 60 28 1 198 257 104 162 2.89e-12 66.6
MS.gene59654.t1 AT5G18240 40.000 90 50 1 168 253 10 99 3.19e-12 67.8
MS.gene59654.t1 AT5G18240 40.000 90 50 1 168 253 10 99 3.19e-12 67.8
MS.gene59654.t1 AT5G18240 40.000 90 50 1 168 253 10 99 3.61e-12 67.4
MS.gene59654.t1 AT5G18240 40.000 90 50 1 168 253 10 99 3.67e-12 67.4
MS.gene59654.t1 AT5G18240 40.000 90 50 1 168 253 10 99 3.67e-12 67.4
MS.gene59654.t1 AT2G20400 59.259 54 22 0 199 252 231 284 3.82e-12 67.4
MS.gene59654.t1 AT2G20400 59.259 54 22 0 199 252 231 284 3.84e-12 67.4
MS.gene59654.t1 AT2G01760 31.707 164 96 5 198 361 199 346 4.60e-12 67.0
MS.gene59654.t1 AT3G16857 47.368 76 34 2 187 257 150 224 4.73e-12 67.8
MS.gene59654.t1 AT2G01760 31.707 164 96 5 198 361 227 374 5.21e-12 67.0
MS.gene59654.t1 AT3G16857 47.368 76 34 2 187 257 220 294 5.37e-12 67.8
MS.gene59654.t1 AT4G13640 58.182 55 23 0 199 253 37 91 5.52e-12 66.2
MS.gene59654.t1 AT5G59570 38.462 104 44 3 154 257 115 198 5.66e-12 66.2
MS.gene59654.t1 AT5G59570 38.462 104 44 3 154 257 115 198 5.66e-12 66.2
MS.gene59654.t1 AT4G13640 58.182 55 23 0 199 253 37 91 6.07e-12 66.2
MS.gene59654.t1 AT2G01760 31.707 164 96 5 198 361 134 281 6.36e-12 66.2
MS.gene59654.t1 AT3G16857 47.368 76 34 2 187 257 220 294 6.80e-12 67.4
MS.gene59654.t1 AT3G24120 58.182 55 23 0 199 253 41 95 7.37e-12 65.9
MS.gene59654.t1 AT3G24120 58.182 55 23 0 199 253 41 95 7.84e-12 65.9
MS.gene59654.t1 AT4G18020 43.820 89 45 3 182 268 277 362 1.28e-11 66.2
MS.gene59654.t1 AT4G18020 43.820 89 45 3 182 268 277 362 1.28e-11 66.2
MS.gene59654.t1 AT4G18020 43.820 89 45 3 182 268 277 362 1.28e-11 66.2
MS.gene59654.t1 AT4G18020 43.820 89 45 3 182 268 277 362 1.28e-11 66.2
MS.gene59654.t1 AT4G18020 43.820 89 45 3 182 268 277 362 1.28e-11 66.2
MS.gene59654.t1 AT4G18020 43.820 89 45 3 182 268 277 362 1.28e-11 66.2
MS.gene59654.t1 AT3G46640 29.825 171 95 4 123 273 52 217 1.38e-11 65.5
MS.gene59654.t1 AT1G79430 58.182 55 23 0 199 253 34 88 1.40e-11 65.5
MS.gene59654.t1 AT3G46640 42.105 76 43 1 198 273 143 217 1.46e-11 65.1
MS.gene59654.t1 AT3G46640 42.105 76 43 1 198 273 143 217 1.46e-11 65.1
MS.gene59654.t1 AT4G18020 43.820 89 45 3 182 268 277 362 1.52e-11 65.9
MS.gene59654.t1 AT4G18020 43.820 89 45 3 182 268 277 362 1.52e-11 65.9
MS.gene59654.t1 AT4G18020 43.820 89 45 3 182 268 277 362 1.52e-11 65.9
MS.gene59654.t1 AT4G18020 43.820 89 45 3 182 268 277 362 1.52e-11 65.9
MS.gene59654.t1 AT4G18020 43.820 89 45 3 182 268 277 362 1.52e-11 65.9
MS.gene59654.t1 AT3G12730 56.364 55 24 0 199 253 23 77 1.73e-11 63.9
MS.gene59654.t1 AT3G04030 41.860 86 46 1 172 253 14 99 2.00e-11 65.1
MS.gene59654.t1 AT4G04605 53.846 52 24 0 201 252 16 67 2.06e-11 63.5
MS.gene59654.t1 AT3G04030 41.860 86 46 1 172 253 14 99 2.15e-11 65.1
MS.gene59654.t1 AT3G04450 55.556 54 24 0 199 252 238 291 2.22e-11 64.7
MS.gene59654.t1 AT5G29000 55.556 54 24 0 199 252 231 284 3.90e-11 63.9
MS.gene59654.t1 AT3G04450 55.556 54 24 0 199 252 198 251 3.97e-11 64.3
MS.gene59654.t1 AT3G04450 55.556 54 24 0 199 252 198 251 3.97e-11 64.3
MS.gene59654.t1 AT3G04450 55.556 54 24 0 199 252 238 291 4.13e-11 64.3
MS.gene59654.t1 AT1G49190 38.462 78 45 1 186 260 202 279 4.96e-11 63.9
MS.gene59654.t1 AT5G06800 50.909 55 27 0 199 253 192 246 5.05e-11 63.2
MS.gene59654.t1 AT3G13040 43.590 78 43 1 176 253 219 295 8.46e-11 63.5
MS.gene59654.t1 AT3G13040 43.590 78 43 1 176 253 219 295 8.46e-11 63.5
MS.gene59654.t1 AT3G13040 43.590 78 43 1 176 253 219 295 8.46e-11 63.5
MS.gene59654.t1 AT5G29000 55.556 54 24 0 199 252 231 284 8.51e-11 63.5
MS.gene59654.t1 AT5G29000 55.556 54 24 0 199 252 188 241 8.89e-11 63.2
MS.gene59654.t1 AT5G29000 55.556 54 24 0 199 252 188 241 8.89e-11 63.2
MS.gene59654.t1 AT4G17695 51.724 58 28 0 195 252 160 217 9.46e-11 62.8

Find 71 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGAATTGTTGTGAATTAT+TGG 0.251586 5.4:-42824749 None:intergenic
TTCTCCTGACACTGTTGTTT+TGG 0.263091 5.4:-42824843 None:intergenic
TTGCTGCAGCTTTCAACAAT+TGG 0.314561 5.4:-42824102 None:intergenic
CAGCAGCGATTTCCTCTTTG+TGG 0.314629 5.4:-42824005 None:intergenic
TTTCAGCATCTTGAAGTTGT+AGG 0.327142 5.4:-42825046 None:intergenic
ATTAGTTCCCGGATCTGTTT+CGG 0.330999 5.4:-42824323 None:intergenic
TAGAATTGTTGTGAATTATT+GGG 0.348297 5.4:-42824748 None:intergenic
TGAAGTCAAAAGTCATTTGC+AGG 0.374794 5.4:+42824370 MS.gene59654:CDS
ACGTGTAAGCAACAGTTGTT+TGG 0.397960 5.4:+42823333 MS.gene59654:CDS
AGAGAAAGAGGAAGGTCTCT+AGG 0.412125 5.4:-42823157 None:intergenic
TATGTCCAACCACAAGAATA+TGG 0.427221 5.4:+42824809 MS.gene59654:CDS
CTGTTGCTTACACGTTTCAA+TGG 0.431651 5.4:-42823325 None:intergenic
TATACCTCTTTAGCAGATGA+AGG 0.432154 5.4:-42823555 None:intergenic
CTGCCACGCCGAAACAGATC+CGG 0.438655 5.4:+42824315 MS.gene59654:CDS
TGGAACACAATCGGAGTGTT+CGG 0.442544 5.4:+42823353 MS.gene59654:CDS
ACCACAGTTACTACTCCAAC+AGG 0.442991 5.4:+42824869 MS.gene59654:CDS
CAACCCTTCATCTGCTAAAG+AGG 0.450187 5.4:+42823551 MS.gene59654:CDS
CATCGACTTTCATTAGTTCC+CGG 0.451625 5.4:-42824334 None:intergenic
AGTTTATTCCTATCAAGAAA+AGG 0.453926 5.4:+42823412 MS.gene59654:CDS
AGCAGGTACTTGAGGCGAAC+TGG 0.464004 5.4:-42824064 None:intergenic
CATGCCCTTCAACAACTTGG+CGG 0.464333 5.4:+42824192 MS.gene59654:CDS
TCTTTAGCAGATGAAGGGTT+GGG 0.465236 5.4:-42823549 None:intergenic
GTTTATTCCTATCAAGAAAA+GGG 0.474072 5.4:+42823413 MS.gene59654:CDS
CACATAAGATGAAATCTGAT+TGG 0.476812 5.4:+42823502 MS.gene59654:CDS
GTCGTAGAAGTGAAGAGAAA+TGG 0.477395 5.4:+42823964 MS.gene59654:CDS
TTGCATGCCCTTCAACAACT+TGG 0.479053 5.4:+42824189 MS.gene59654:CDS
TACAGCTCCTATGTTCTTAG+TGG 0.486260 5.4:+42824778 MS.gene59654:CDS
AGAGGAAGCAGCGTCGGTGC+TGG 0.486844 5.4:+42824142 MS.gene59654:CDS
CTCTTTAGCAGATGAAGGGT+TGG 0.487382 5.4:-42823550 None:intergenic
GCCACCAAAACAACAGTGTC+AGG 0.487737 5.4:+42824839 MS.gene59654:CDS
ATAACTGTTGTTGTAGATGA+AGG 0.491980 5.4:-42824917 None:intergenic
TTGAACCGCCAAGTTGTTGA+AGG 0.494195 5.4:-42824197 None:intergenic
GAGAAAGAGGAAGGTCTCTA+GGG 0.495572 5.4:-42823156 None:intergenic
GCGATTTCCTCTTTGTGGAA+TGG 0.495901 5.4:-42824000 None:intergenic
ACAGTTGTTTGGAACACAAT+CGG 0.495980 5.4:+42823344 MS.gene59654:CDS
GTAACAAGCTCAAGAGAAAG+AGG 0.505480 5.4:-42823169 None:intergenic
ACAAGAAAAGAAGAAAATCC+AGG 0.515271 5.4:+42823129 MS.gene59654:CDS
CTGCACCATATTCTTGTGGT+TGG 0.520262 5.4:-42824814 None:intergenic
GAAGGTCTCTAGGGAAAACC+TGG 0.528167 5.4:-42823147 None:intergenic
TGCCACGCCGAAACAGATCC+GGG 0.529744 5.4:+42824316 MS.gene59654:CDS
GGCTAAGATCTGTTCAGTTG+TGG 0.535273 5.4:+42823523 MS.gene59654:CDS
ATTTCAACCATTCCACAAAG+AGG 0.547227 5.4:+42823993 MS.gene59654:CDS
ACAGCTCCTATGTTCTTAGT+GGG 0.553648 5.4:+42824779 MS.gene59654:CDS
GAACAGACATCAACTGATGA+AGG 0.553875 5.4:+42823375 MS.gene59654:CDS
ATACCTCTTTAGCAGATGAA+GGG 0.556739 5.4:-42823554 None:intergenic
TCGTGACTATATCCTAGCAT+TGG 0.561341 5.4:+42823105 MS.gene59654:CDS
TGAACCGCCAAGTTGTTGAA+GGG 0.566682 5.4:-42824196 None:intergenic
GCTACTGCACCATATTCTTG+TGG 0.569246 5.4:-42824818 None:intergenic
GGTTCAGTAGAACTTGTAGC+AGG 0.569250 5.4:-42824081 None:intergenic
TGAGTGGAACATGATGAGGC+AGG 0.570388 5.4:-42825019 None:intergenic
GAGTGGAACATGATGAGGCA+GGG 0.580949 5.4:-42825018 None:intergenic
ATGTTCCCCACTAAGAACAT+AGG 0.582533 5.4:-42824785 None:intergenic
ACAGGTGCATAAATTCCTGT+TGG 0.585331 5.4:-42824884 None:intergenic
CAAGCTCAAGAGAAAGAGGA+AGG 0.593272 5.4:-42823165 None:intergenic
AGTGGAACATGATGAGGCAG+GGG 0.594079 5.4:-42825017 None:intergenic
AACAGGAATTTATGCACCTG+TGG 0.600669 5.4:+42824886 MS.gene59654:CDS
GAACTTGTAGCAGGTACTTG+AGG 0.602704 5.4:-42824072 None:intergenic
AATTTCGTCTGCACACGAGA+AGG 0.610345 5.4:+42824723 MS.gene59654:CDS
TCTCTTGAGCTTGTTACACA+AGG 0.614804 5.4:+42823175 MS.gene59654:CDS
AAGGTCAGAGGAAGCAGCGT+CGG 0.617450 5.4:+42824136 MS.gene59654:CDS
CGGGAACTAATGAAAGTCGA+TGG 0.628624 5.4:+42824335 MS.gene59654:CDS
GGTACTTGAGGCGAACTGGT+TGG 0.629043 5.4:-42824060 None:intergenic
TGTTTGTTCAGGATGTACCT+AGG 0.649194 5.4:+42823932 MS.gene59654:intron
TGAAGTTGTAGGTGTATGAG+TGG 0.650979 5.4:-42825035 None:intergenic
GTAGAAGTGAAGAGAAATGG+TGG 0.664268 5.4:+42823967 MS.gene59654:CDS
GATGAAGGATGTGTAGCCAC+AGG 0.668784 5.4:-42824902 None:intergenic
CAATTGCGATGAAAATGATG+AGG 0.674430 5.4:+42823446 MS.gene59654:CDS
TCCTGTTGGAGTAGTAACTG+TGG 0.676951 5.4:-42824870 None:intergenic
CAGCTCCTATGTTCTTAGTG+GGG 0.686803 5.4:+42824780 MS.gene59654:CDS
TTCCCGGATCTGTTTCGGCG+TGG 0.690024 5.4:-42824318 None:intergenic
TGTATGAGTGGAACATGATG+AGG 0.702899 5.4:-42825023 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTTATTAAAATCAACTT+TGG - chr5.4:42823695-42823714 None:intergenic 10.0%
!!! ATTTTTTCTTTGTTTCATAA+TGG + chr5.4:42823885-42823904 MS.gene59654:intron 15.0%
!!! GTCTAACTTTTTTTAATTTT+CGG + chr5.4:42824689-42824708 MS.gene59654:intron 15.0%
!!! TCAAATTTTTTCTTAAACTT+TGG - chr5.4:42824947-42824966 None:intergenic 15.0%
!! GACAAGTAAAATTATACAAA+TGG - chr5.4:42824430-42824449 None:intergenic 20.0%
!! TAGAATTGTTGTGAATTATT+GGG - chr5.4:42824751-42824770 None:intergenic 20.0%
!! TCTTCTTGATTGTTAAATTA+TGG + chr5.4:42824667-42824686 MS.gene59654:intron 20.0%
!! TTAATCATAGAAAATTACAC+TGG + chr5.4:42823614-42823633 MS.gene59654:intron 20.0%
!! TTAGAATTGTTGTGAATTAT+TGG - chr5.4:42824752-42824771 None:intergenic 20.0%
!!! GATATATTTTAAGCTTGATT+TGG - chr5.4:42824542-42824561 None:intergenic 20.0%
!!! GTGTATATTTTTTTTTCTTC+TGG - chr5.4:42823213-42823232 None:intergenic 20.0%
! AAAAAATCAAAACGATGAAG+TGG - chr5.4:42824404-42824423 None:intergenic 25.0%
! ACACAATTCACATTTAGATT+CGG - chr5.4:42824281-42824300 None:intergenic 25.0%
! AGTTTATTCCTATCAAGAAA+AGG + chr5.4:42823412-42823431 MS.gene59654:CDS 25.0%
! CAAGTTAATGTTTCTTTCAT+TGG + chr5.4:42823245-42823264 MS.gene59654:intron 25.0%
! CAATGAAAGAAACATTAACT+TGG - chr5.4:42823247-42823266 None:intergenic 25.0%
! GTTTATTCCTATCAAGAAAA+GGG + chr5.4:42823413-42823432 MS.gene59654:CDS 25.0%
!! AGAATTTGAAATGTTTGTTC+AGG + chr5.4:42823921-42823940 MS.gene59654:intron 25.0%
!!! CTTTTTTTAATTTTCGGACA+TGG + chr5.4:42824695-42824714 MS.gene59654:intron 25.0%
ACAAGAAAAGAAGAAAATCC+AGG + chr5.4:42823129-42823148 MS.gene59654:CDS 30.0%
AGATGTCTAATCTTCATCTA+CGG - chr5.4:42824504-42824523 None:intergenic 30.0%
ATAACTGTTGTTGTAGATGA+AGG - chr5.4:42824920-42824939 None:intergenic 30.0%
CACATAAGATGAAATCTGAT+TGG + chr5.4:42823502-42823521 MS.gene59654:CDS 30.0%
CGTACAAAAAAATTCGACTT+TGG - chr5.4:42823592-42823611 None:intergenic 30.0%
CTGTCTAACCTTTAATCTAA+CGG + chr5.4:42824573-42824592 MS.gene59654:intron 30.0%
GAATAAACTCCTCAAAAACA+AGG - chr5.4:42823401-42823420 None:intergenic 30.0%
TTACTAACCACGTATAATCT+TGG - chr5.4:42824458-42824477 None:intergenic 30.0%
! TCATCATTTTCATCGCAATT+GGG - chr5.4:42823447-42823466 None:intergenic 30.0%
!! TGTTTCTTTCATTGGTTTCT+TGG + chr5.4:42823253-42823272 MS.gene59654:intron 30.0%
AAAAAGACGATAAAGGTCAG+AGG + chr5.4:42824124-42824143 MS.gene59654:CDS 35.0%
ATACCTCTTTAGCAGATGAA+GGG - chr5.4:42823557-42823576 None:intergenic 35.0%
ATTTCAACCATTCCACAAAG+AGG + chr5.4:42823993-42824012 MS.gene59654:CDS 35.0%
CAATTGCGATGAAAATGATG+AGG + chr5.4:42823446-42823465 MS.gene59654:CDS 35.0%
GAAAATTACACTGGACTGAA+AGG + chr5.4:42823623-42823642 MS.gene59654:intron 35.0%
GATCTTGACCGTTAGATTAA+AGG - chr5.4:42824584-42824603 None:intergenic 35.0%
GTTTGATAAATTGAGTGACG+CGG - chr5.4:42823793-42823812 None:intergenic 35.0%
TATACCTCTTTAGCAGATGA+AGG - chr5.4:42823558-42823577 None:intergenic 35.0%
TATGTCCAACCACAAGAATA+TGG + chr5.4:42824809-42824828 MS.gene59654:CDS 35.0%
TGAAGTCAAAAGTCATTTGC+AGG + chr5.4:42824370-42824389 MS.gene59654:CDS 35.0%
TGCAGCAAAAAAGACGATAA+AGG + chr5.4:42824117-42824136 MS.gene59654:CDS 35.0%
! ACAGTTGTTTGGAACACAAT+CGG + chr5.4:42823344-42823363 MS.gene59654:CDS 35.0%
! ATCATTTTCATCGCAATTGG+GGG - chr5.4:42823445-42823464 None:intergenic 35.0%
! CATCATTTTCATCGCAATTG+GGG - chr5.4:42823446-42823465 None:intergenic 35.0%
! CTCATCATTTTCATCGCAAT+TGG - chr5.4:42823448-42823467 None:intergenic 35.0%
! GAACTGTGAAACTTTTGATC+AGG + chr5.4:42823760-42823779 MS.gene59654:intron 35.0%
! TCTTTTCTTGTTCCAATGCT+AGG - chr5.4:42823120-42823139 None:intergenic 35.0%
!! TCTACGACATTTGTTTTCCT+AGG - chr5.4:42823952-42823971 None:intergenic 35.0%
!!! ATGACTTTTGACTTCATCGT+TGG - chr5.4:42824366-42824385 None:intergenic 35.0%
AACAGGAATTTATGCACCTG+TGG + chr5.4:42824886-42824905 MS.gene59654:CDS 40.0%
ACAGCTCCTATGTTCTTAGT+GGG + chr5.4:42824779-42824798 MS.gene59654:CDS 40.0%
ACAGGTGCATAAATTCCTGT+TGG - chr5.4:42824887-42824906 None:intergenic 40.0%
ACATCAAGCAAGCAGACAAT+AGG - chr5.4:42823815-42823834 None:intergenic 40.0%
ACGTGTAAGCAACAGTTGTT+TGG + chr5.4:42823333-42823352 MS.gene59654:CDS 40.0%
AGTCGCAATTCTGATAGTCA+AGG - chr5.4:42824606-42824625 None:intergenic 40.0%
ATGTTCCCCACTAAGAACAT+AGG - chr5.4:42824788-42824807 None:intergenic 40.0%
ATTAGTTCCCGGATCTGTTT+CGG - chr5.4:42824326-42824345 None:intergenic 40.0%
GAACAGACATCAACTGATGA+AGG + chr5.4:42823375-42823394 MS.gene59654:CDS 40.0%
GTAACAAGCTCAAGAGAAAG+AGG - chr5.4:42823172-42823191 None:intergenic 40.0%
GTAGAAGTGAAGAGAAATGG+TGG + chr5.4:42823967-42823986 MS.gene59654:CDS 40.0%
GTCGTAGAAGTGAAGAGAAA+TGG + chr5.4:42823964-42823983 MS.gene59654:CDS 40.0%
TACAGCTCCTATGTTCTTAG+TGG + chr5.4:42824778-42824797 MS.gene59654:CDS 40.0%
TCATCTACGGTAAAAATCCG+TGG - chr5.4:42824491-42824510 None:intergenic 40.0%
TCGTGACTATATCCTAGCAT+TGG + chr5.4:42823105-42823124 MS.gene59654:CDS 40.0%
TCTCAGACCAAGATTATACG+TGG + chr5.4:42824448-42824467 MS.gene59654:intron 40.0%
TCTTTAGCAGATGAAGGGTT+GGG - chr5.4:42823552-42823571 None:intergenic 40.0%
TGAAGTTGTAGGTGTATGAG+TGG - chr5.4:42825038-42825057 None:intergenic 40.0%
TGTATGAGTGGAACATGATG+AGG - chr5.4:42825026-42825045 None:intergenic 40.0%
TGTTTGTTCAGGATGTACCT+AGG + chr5.4:42823932-42823951 MS.gene59654:intron 40.0%
TTAGTAAACCCTGCAATCCA+CGG + chr5.4:42824471-42824490 MS.gene59654:intron 40.0%
TTGCTGCAGCTTTCAACAAT+TGG - chr5.4:42824105-42824124 None:intergenic 40.0%
! CTGTTGCTTACACGTTTCAA+TGG - chr5.4:42823328-42823347 None:intergenic 40.0%
! GACAATGCCCTTTTCTTGAT+AGG - chr5.4:42823423-42823442 None:intergenic 40.0%
! GTAAAAATCCGTGGATTGCA+GGG - chr5.4:42824482-42824501 None:intergenic 40.0%
!! CATCGACTTTCATTAGTTCC+CGG - chr5.4:42824337-42824356 None:intergenic 40.0%
!! TCTCTTGAGCTTGTTACACA+AGG + chr5.4:42823175-42823194 MS.gene59654:CDS 40.0%
!! TTCTCCTGACACTGTTGTTT+TGG - chr5.4:42824846-42824865 None:intergenic 40.0%
!!! TGATGAAGGCCTTGTTTTTG+AGG + chr5.4:42823389-42823408 MS.gene59654:CDS 40.0%
!!! TTGGTTTTGCCATCCAGAAA+CGG - chr5.4:42823676-42823695 None:intergenic 40.0%
!!! TTTTGACTTCATCGTTGGTG+AGG - chr5.4:42824361-42824380 None:intergenic 40.0%
AATTTCGTCTGCACACGAGA+AGG + chr5.4:42824723-42824742 MS.gene59654:CDS 45.0%
ACCACAGTTACTACTCCAAC+AGG + chr5.4:42824869-42824888 MS.gene59654:CDS 45.0%
AGAGAAAGAGGAAGGTCTCT+AGG - chr5.4:42823160-42823179 None:intergenic 45.0%
CAACCCTTCATCTGCTAAAG+AGG + chr5.4:42823551-42823570 MS.gene59654:CDS 45.0%
CAAGCTCAAGAGAAAGAGGA+AGG - chr5.4:42823168-42823187 None:intergenic 45.0%
CAGCTCCTATGTTCTTAGTG+GGG + chr5.4:42824780-42824799 MS.gene59654:CDS 45.0%
CGGGAACTAATGAAAGTCGA+TGG + chr5.4:42824335-42824354 MS.gene59654:CDS 45.0%
CTCTTTAGCAGATGAAGGGT+TGG - chr5.4:42823553-42823572 None:intergenic 45.0%
CTGCACCATATTCTTGTGGT+TGG - chr5.4:42824817-42824836 None:intergenic 45.0%
GAACTTGTAGCAGGTACTTG+AGG - chr5.4:42824075-42824094 None:intergenic 45.0%
GAGAAAGAGGAAGGTCTCTA+GGG - chr5.4:42823159-42823178 None:intergenic 45.0%
GCGATTTCCTCTTTGTGGAA+TGG - chr5.4:42824003-42824022 None:intergenic 45.0%
GCTACTGCACCATATTCTTG+TGG - chr5.4:42824821-42824840 None:intergenic 45.0%
GGCTAAGATCTGTTCAGTTG+TGG + chr5.4:42823523-42823542 MS.gene59654:CDS 45.0%
GGTTCAGTAGAACTTGTAGC+AGG - chr5.4:42824084-42824103 None:intergenic 45.0%
TCCTGTTGGAGTAGTAACTG+TGG - chr5.4:42824873-42824892 None:intergenic 45.0%
TGAACCGCCAAGTTGTTGAA+GGG - chr5.4:42824199-42824218 None:intergenic 45.0%
TTGAACCGCCAAGTTGTTGA+AGG - chr5.4:42824200-42824219 None:intergenic 45.0%
TTGCATGCCCTTCAACAACT+TGG + chr5.4:42824189-42824208 MS.gene59654:CDS 45.0%
! GGTAAAAATCCGTGGATTGC+AGG - chr5.4:42824483-42824502 None:intergenic 45.0%
! TGGAACACAATCGGAGTGTT+CGG + chr5.4:42823353-42823372 MS.gene59654:CDS 45.0%
!!! TCCTGACACTGTTGTTTTGG+TGG - chr5.4:42824843-42824862 None:intergenic 45.0%
ACACTGGACTGAAAGGCAAG+CGG + chr5.4:42823630-42823649 MS.gene59654:intron 50.0%
AGTGGAACATGATGAGGCAG+GGG - chr5.4:42825020-42825039 None:intergenic 50.0%
CAGCAGCGATTTCCTCTTTG+TGG - chr5.4:42824008-42824027 None:intergenic 50.0%
CATGCCCTTCAACAACTTGG+CGG + chr5.4:42824192-42824211 MS.gene59654:CDS 50.0%
GAGTGGAACATGATGAGGCA+GGG - chr5.4:42825021-42825040 None:intergenic 50.0%
GATGAAGGATGTGTAGCCAC+AGG - chr5.4:42824905-42824924 None:intergenic 50.0%
GCCACCAAAACAACAGTGTC+AGG + chr5.4:42824839-42824858 MS.gene59654:CDS 50.0%
TGAGTGGAACATGATGAGGC+AGG - chr5.4:42825022-42825041 None:intergenic 50.0%
TGCCATCCAGAAACGGTTAG+CGG - chr5.4:42823669-42823688 None:intergenic 50.0%
! GAAGGTCTCTAGGGAAAACC+TGG - chr5.4:42823150-42823169 None:intergenic 50.0%
AAGGTCAGAGGAAGCAGCGT+CGG + chr5.4:42824136-42824155 MS.gene59654:CDS 55.0%
AGCAGGTACTTGAGGCGAAC+TGG - chr5.4:42824067-42824086 None:intergenic 55.0%
GGTACTTGAGGCGAACTGGT+TGG - chr5.4:42824063-42824082 None:intergenic 55.0%
! GTTGTGCCGCTAACCGTTTC+TGG + chr5.4:42823660-42823679 MS.gene59654:intron 55.0%
! TGCCGCTAACCGTTTCTGGA+TGG + chr5.4:42823664-42823683 MS.gene59654:intron 55.0%
CTGCCACGCCGAAACAGATC+CGG + chr5.4:42824315-42824334 MS.gene59654:CDS 60.0%
TGCCACGCCGAAACAGATCC+GGG + chr5.4:42824316-42824335 MS.gene59654:CDS 60.0%
TTCCCGGATCTGTTTCGGCG+TGG - chr5.4:42824321-42824340 None:intergenic 60.0%
!! AGAGGAAGCAGCGTCGGTGC+TGG + chr5.4:42824142-42824161 MS.gene59654:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 42823055 42825066 42823055 ID=MS.gene59654
chr5.4 mRNA 42823055 42825066 42823055 ID=MS.gene59654.t1;Parent=MS.gene59654
chr5.4 exon 42823055 42823196 42823055 ID=MS.gene59654.t1.exon1;Parent=MS.gene59654.t1
chr5.4 CDS 42823055 42823196 42823055 ID=cds.MS.gene59654.t1;Parent=MS.gene59654.t1
chr5.4 exon 42823325 42823572 42823325 ID=MS.gene59654.t1.exon2;Parent=MS.gene59654.t1
chr5.4 CDS 42823325 42823572 42823325 ID=cds.MS.gene59654.t1;Parent=MS.gene59654.t1
chr5.4 exon 42823943 42824222 42823943 ID=MS.gene59654.t1.exon3;Parent=MS.gene59654.t1
chr5.4 CDS 42823943 42824222 42823943 ID=cds.MS.gene59654.t1;Parent=MS.gene59654.t1
chr5.4 exon 42824315 42824391 42824315 ID=MS.gene59654.t1.exon4;Parent=MS.gene59654.t1
chr5.4 CDS 42824315 42824391 42824315 ID=cds.MS.gene59654.t1;Parent=MS.gene59654.t1
chr5.4 exon 42824722 42825066 42824722 ID=MS.gene59654.t1.exon5;Parent=MS.gene59654.t1
chr5.4 CDS 42824722 42825066 42824722 ID=cds.MS.gene59654.t1;Parent=MS.gene59654.t1
Gene Sequence

>MS.gene59654

ATGGTTTCAATGCAATTCGATGATAAAATGCACTCGCATAAAATGAAATTTCGTGACTATATCCTAGCATTGGAACAAGAAAAGAAGAAAATCCAGGTTTTCCCTAGAGACCTTCCTCTTTCTCTTGAGCTTGTTACACAAGGTAAACGAACAAACCAGAAGAAAAAAAAATATACACTTTGTTTCATACCAAGTTAATGTTTCTTTCATTGGTTTCTTGGTCTGTGTTTTTCGACTTTTTTTTATATGTTATGTTTTGTGAATATGCAGCCATTGAAACGTGTAAGCAACAGTTGTTTGGAACACAATCGGAGTGTTCGGAACAGACATCAACTGATGAAGGCCTTGTTTTTGAGGAGTTTATTCCTATCAAGAAAAGGGCATTGTCCCCCAATTGCGATGAAAATGATGAGGAAGATGATGATGATAATGATGAGCAACATTCATCACATAAGATGAAATCTGATTGGCTAAGATCTGTTCAGTTGTGGAATCCCAACCCTTCATCTGCTAAAGAGGTATAAAATTGCAAAACCAAAGTCGAATTTTTTTGTACGATTTAATCATAGAAAATTACACTGGACTGAAAGGCAAGCGGACAACAAGTTGTGCCGCTAACCGTTTCTGGATGGCAAAACCAAAGTTGATTTTAATAAATAAATGAATTTTAATTTTAGAAGCAAACGTTAGTAAATTAGCGATTTTGAACTGTGAAACTTTTGATCAGGACTTCTTCCGCGTCACTCAATTTATCAAACCTATTGTCTGCTTGCTTGATGTTGAACAATCACTTACTCAACAGATACATTGAATGAAATTGTTGCTTTTGAATTTTTTCTTTGTTTCATAATGGAAAAGAAATCAAGAGAATTTGAAATGTTTGTTCAGGATGTACCTAGGAAAACAAATGTCGTAGAAGTGAAGAGAAATGGTGGAGCATTTCAACCATTCCACAAAGAGGAAATCGCTGCTGAAAAAGATAATGCGTTAGAAAGTGATAAAGCACCAACCAGTTCGCCTCAAGTACCTGCTACAAGTTCTACTGAACCAATTGTTGAAAGCTGCAGCAAAAAAGACGATAAAGGTCAGAGGAAGCAGCGTCGGTGCTGGTCACAAGAATTGCACAAACGCTTCTTGCATGCCCTTCAACAACTTGGCGGTTCAAATTGTGAGTGAATGTATTACTCATCAGCATAATTAGTTCATTCTGATTGTTAATTGTTCCGAATCTAAATGTGAATTGTGTGTGACATGTTGTAGCTGCCACGCCGAAACAGATCCGGGAACTAATGAAAGTCGATGGCCTCACCAACGATGAAGTCAAAAGTCATTTGCAGGTTAGTTTACCACTTCATCGTTTTGATTTTTTGTTCCATTTGTATAATTTTACTTGTCTCAGACCAAGATTATACGTGGTTAGTAAACCCTGCAATCCACGGATTTTTACCGTAGATGAAGATTAGACATCTCGTATTCTGATTATACCAAATCAAGCTTAAAATATATCTCGAATACGAGCTGTCTAACCTTTAATCTAACGGTCAAGATCCTTGACTATCAGAATTGCGACTGCATAGAACTCGAATTCATATTAGCAGATATCACTATACGATCTTCTTGATTGTTAAATTATGGTCTAACTTTTTTTAATTTTCGGACATGGACAGAAATTTCGTCTGCACACGAGAAGGAGCCCAATAATTCACAACAATTCTAATTCACATACAGCTCCTATGTTCTTAGTGGGGAACATTTATGTCCAACCACAAGAATATGGTGCAGTAGCCACCAAAACAACAGTGTCAGGAGAATTGACCACAGTTACTACTCCAACAGGAATTTATGCACCTGTGGCTACACATCCTTCATCTACAACAACAGTTATAAAACCAAAGTTTAAGAAAAAATTTGAGTTGAGTGAGAATTCACATTCTGTAGAAAGAATTGTTGCTCATTCTAACTCCCCTGCCTCATCATGTTCCACTCATACACCTACAACTTCAAGATGCTGA
Protein sequence

>MS.gene59654.t1

MVSMQFDDKMHSHKMKFRDYILALEQEKKKIQVFPRDLPLSLELVTQAIETCKQQLFGTQSECSEQTSTDEGLVFEEFIPIKKRALSPNCDENDEEDDDDNDEQHSSHKMKSDWLRSVQLWNPNPSSAKEDVPRKTNVVEVKRNGGAFQPFHKEEIAAEKDNALESDKAPTSSPQVPATSSTEPIVESCSKKDDKGQRKQRRCWSQELHKRFLHALQQLGGSNSATPKQIRELMKVDGLTNDEVKSHLQKFRLHTRRSPIIHNNSNSHTAPMFLVGNIYVQPQEYGAVATKTTVSGELTTVTTPTGIYAPVATHPSSTTTVIKPKFKKKFELSENSHSVERIVAHSNSPASSCSTHTPTTSRC