AlfalfaGEDB | MS.gene59683

M. sativa cultivar XinJiangDaYe / MS.gene59683

KEGG: K02156
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59683.t1	AES99247.1	36.3	237	138	4	3	228	75	309	2.10E-34	156

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59683.t1	G7K1P9	36.3	237	138	4	3	228	75	309	1.5e-34	156.0

TFs/TRs:

Gene ID	Type	Classification
MS.gene59683.t1	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59683.t1	MTR_5g078270	36.975	238	137	5	2	228	74	309	2.84e-43	148
MS.gene59683.t1	MTR_0010s0290	33.333	237	138	6	3	228	97	324	1.88e-36	130
MS.gene59683.t1	MTR_0010s0320	33.193	238	139	6	2	228	65	293	2.71e-36	129
MS.gene59683.t1	MTR_1g054480	32.468	231	143	5	3	229	74	295	6.77e-29	110
MS.gene59683.t1	MTR_0010s0310	34.434	212	99	5	8	217	67	240	2.25e-26	102
MS.gene59683.t1	MTR_2g012700	30.189	106	73	1	122	226	14	119	4.11e-13	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 66 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGAACGACGTACCATATTT+TGG	0.218433	5.4:+43407081	MS.gene59683:CDS
CATCTGATAATTGTAGAAAT+TGG	0.239747	5.4:-43407275	None:intergenic
CCGAGGTATGTTGATGTTAT+TGG	0.249280	5.4:+43407165	MS.gene59683:CDS
AGTCTTCAATCTTAGACTTT+TGG	0.299655	5.4:-43407672	None:intergenic
TATATGGTTGACTACAGATT+TGG	0.309041	5.4:-43407522	None:intergenic
TTCAATATGCGAATTGTTGA+TGG	0.313877	5.4:+43407054	MS.gene59683:CDS
CCTTTGAGTTTGCAATGTTA+TGG	0.330298	5.4:+43407214	MS.gene59683:CDS
GGATAATGGAATCCAAAATA+TGG	0.331913	5.4:-43407093	None:intergenic
ATATGGTTGACTACAGATTT+GGG	0.342902	5.4:-43407521	None:intergenic
CTCCAATGGAACTCAATGTT+AGG	0.348995	5.4:+43407726	MS.gene59683:CDS
GGCCGACCATAACATAGTTT+TGG	0.363792	5.4:+43407553	MS.gene59683:CDS
CTCGGATTGACTTGAAGGTT+TGG	0.372952	5.4:-43407147	None:intergenic
CATACCTCGGATTGACTTGA+AGG	0.392440	5.4:-43407152	None:intergenic
TCACTTTGGCCCTTAGCAAC+TGG	0.405712	5.4:-43407372	None:intergenic
GGTCGGCCACAAAAGGTATA+TGG	0.409259	5.4:-43407538	None:intergenic
GGGAGGAAAAGACCACCGTC+AGG	0.427903	5.4:-43407192	None:intergenic
CAACAATTCGCATATTGAAC+TGG	0.435687	5.4:-43407050	None:intergenic
CATAACATTGCAAACTCAAA+GGG	0.444223	5.4:-43407213	None:intergenic
CACAGTACCTTGCAATAATC+CGG	0.462888	5.4:-43406978	None:intergenic
CATCAACCAAAACTATGTTA+TGG	0.483266	5.4:-43407559	None:intergenic
TGTAACCAGGCCAGTTGCTA+AGG	0.483856	5.4:+43407362	MS.gene59683:CDS
ACCCTAACATTGAGTTCCAT+TGG	0.485090	5.4:-43407728	None:intergenic
CAATTTACATAGGGAGTGGC+TGG	0.490021	5.4:+43407639	MS.gene59683:CDS
ATGTTAGGGTTCTCCGTCGC+AGG	0.496923	5.4:+43407741	MS.gene59683:CDS
TTGGCCCTTAGCAACTGGCC+TGG	0.499222	5.4:-43407367	None:intergenic
TCCAATGGAACTCAATGTTA+GGG	0.501785	5.4:+43407727	MS.gene59683:CDS
TGCTAAGGGCCAAAGTGACT+TGG	0.502196	5.4:+43407377	MS.gene59683:CDS
ATAATTGTAGAAATTGGATA+AGG	0.509582	5.4:-43407269	None:intergenic
ATGCGAATTGTTGATGGAGA+AGG	0.509644	5.4:+43407060	MS.gene59683:CDS
ATGTTATGGTCGGCCACAAA+AGG	0.511329	5.4:-43407545	None:intergenic
TTATGGATGGAAGAACTTAA+CGG	0.519426	5.4:+43407231	MS.gene59683:CDS
CCATAACATTGCAAACTCAA+AGG	0.523655	5.4:-43407214	None:intergenic
TCGGAAGAAGCAATTTACAT+AGG	0.532988	5.4:+43407629	MS.gene59683:CDS
GAGGAAAAGACCACCGTCAG+GGG	0.542442	5.4:-43407190	None:intergenic
CGGAAGAAGCAATTTACATA+GGG	0.544470	5.4:+43407630	MS.gene59683:CDS
GGAGGAAAAGACCACCGTCA+GGG	0.553757	5.4:-43407191	None:intergenic
GTATCCATCGATACTTTGCA+TGG	0.555610	5.4:-43407602	None:intergenic
CGGATTATTGCAAGGTACTG+TGG	0.558040	5.4:+43406979	MS.gene59683:CDS
TGCGAATTGTTGATGGAGAA+GGG	0.561259	5.4:+43407061	MS.gene59683:CDS
TAGTCAAGAATTACCTTCTG+CGG	0.561863	5.4:-43407012	None:intergenic
GTGTACTAACCAAGTCACTT+TGG	0.573250	5.4:-43407386	None:intergenic
AACTGGCCTGGTTACATCCA+AGG	0.574011	5.4:-43407355	None:intergenic
CTAACATTGAGTTCCATTGG+AGG	0.582212	5.4:-43407725	None:intergenic
AAAAGTCTAAGATTGAAGAC+TGG	0.584520	5.4:+43407674	MS.gene59683:CDS
ACATGTTCATCAGAGGCGGC+CGG	0.584533	5.4:+43406959	None:intergenic
TTGTCTGACATGTTCATCAG+AGG	0.585532	5.4:+43406952	None:intergenic
AGGAAAAGACCACCGTCAGG+GGG	0.587565	5.4:-43407189	None:intergenic
AGATGAACATAATGAGACTT+CGG	0.590070	5.4:+43407293	MS.gene59683:CDS
TGGACTGGACAATCCTCCAA+TGG	0.590635	5.4:+43407712	MS.gene59683:CDS
GAAGCAATTTACATAGGGAG+TGG	0.592040	5.4:+43407635	MS.gene59683:CDS
TCTGACATGTTCATCAGAGG+CGG	0.593190	5.4:+43406955	None:intergenic
TGGTTTCAATGAACCGCAGA+AGG	0.596257	5.4:+43406999	MS.gene59683:CDS
AGTGGACCTTGGATGTAACC+AGG	0.598459	5.4:+43407349	MS.gene59683:CDS
ATTCCCATGCAAAGTATCGA+TGG	0.599282	5.4:+43407598	MS.gene59683:CDS
TGAGTTTGCAATGTTATGGA+TGG	0.599799	5.4:+43407218	MS.gene59683:CDS
AACCAAAACTATGTTATGGT+CGG	0.603595	5.4:-43407555	None:intergenic
TATCCATCGATACTTTGCAT+GGG	0.609143	5.4:-43407601	None:intergenic
CCAATAACATCAACATACCT+CGG	0.630235	5.4:-43407165	None:intergenic
GTAACCAGGCCAGTTGCTAA+GGG	0.632798	5.4:+43407363	MS.gene59683:CDS
TGCTTCAGTGAGACCTGCGA+CGG	0.645760	5.4:-43407754	None:intergenic
GAGGCGGCCGGATTATTGCA+AGG	0.655116	5.4:+43406971	MS.gene59683:CDS
CAAACCTTCAAGTCAATCCG+AGG	0.662887	5.4:+43407148	MS.gene59683:CDS
TATGGTTGACTACAGATTTG+GGG	0.664603	5.4:-43407520	None:intergenic
ACATTGCAAACTCAAAGGGG+AGG	0.676366	5.4:-43407209	None:intergenic
AGTATCGATGGATACAACAT+CGG	0.685684	5.4:+43407610	MS.gene59683:CDS
ATAACATTGCAAACTCAAAG+GGG	0.714085	5.4:-43407212	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAATTGTAGAAATTGGATA+AGG	-	chr5.4:43407272-43407291	None:intergenic	20.0%
!!	ATATGTTTGTTTAAATATGC+AGG	-	chr5.4:43407483-43407502	None:intergenic	20.0%
!	CATCTGATAATTGTAGAAAT+TGG	-	chr5.4:43407278-43407297	None:intergenic	25.0%
!!	ATCTTAGCATGAATATTTTC+AGG	-	chr5.4:43407117-43407136	None:intergenic	25.0%
!!!	CATGAATATTTTCAGGATAA+TGG	-	chr5.4:43407110-43407129	None:intergenic	25.0%
	AACCAAAACTATGTTATGGT+CGG	-	chr5.4:43407558-43407577	None:intergenic	30.0%
	AGATGAACATAATGAGACTT+CGG	+	chr5.4:43407293-43407312	MS.gene59683:CDS	30.0%
	ATAACATTGCAAACTCAAAG+GGG	-	chr5.4:43407215-43407234	None:intergenic	30.0%
	ATATGGTTGACTACAGATTT+GGG	-	chr5.4:43407524-43407543	None:intergenic	30.0%
	CATAACATTGCAAACTCAAA+GGG	-	chr5.4:43407216-43407235	None:intergenic	30.0%
	CATCAACCAAAACTATGTTA+TGG	-	chr5.4:43407562-43407581	None:intergenic	30.0%
	TATATGGTTGACTACAGATT+TGG	-	chr5.4:43407525-43407544	None:intergenic	30.0%
	TTATGGATGGAAGAACTTAA+CGG	+	chr5.4:43407231-43407250	MS.gene59683:CDS	30.0%
	TTCAATATGCGAATTGTTGA+TGG	+	chr5.4:43407054-43407073	MS.gene59683:CDS	30.0%
!	AAAAGTCTAAGATTGAAGAC+TGG	+	chr5.4:43407674-43407693	MS.gene59683:CDS	30.0%
!	AGTCTTCAATCTTAGACTTT+TGG	-	chr5.4:43407675-43407694	None:intergenic	30.0%
!!	GGATAATGGAATCCAAAATA+TGG	-	chr5.4:43407096-43407115	None:intergenic	30.0%
	CAACAATTCGCATATTGAAC+TGG	-	chr5.4:43407053-43407072	None:intergenic	35.0%
	CCAATAACATCAACATACCT+CGG	-	chr5.4:43407168-43407187	None:intergenic	35.0%
	CCATAACATTGCAAACTCAA+AGG	-	chr5.4:43407217-43407236	None:intergenic	35.0%
	CGGAAGAAGCAATTTACATA+GGG	+	chr5.4:43407630-43407649	MS.gene59683:CDS	35.0%
	TAGTCAAGAATTACCTTCTG+CGG	-	chr5.4:43407015-43407034	None:intergenic	35.0%
	TATCCATCGATACTTTGCAT+GGG	-	chr5.4:43407604-43407623	None:intergenic	35.0%
	TATGGTTGACTACAGATTTG+GGG	-	chr5.4:43407523-43407542	None:intergenic	35.0%
	TCCAATGGAACTCAATGTTA+GGG	+	chr5.4:43407727-43407746	MS.gene59683:CDS	35.0%
	TCGGAAGAAGCAATTTACAT+AGG	+	chr5.4:43407629-43407648	MS.gene59683:CDS	35.0%
	TGAGTTTGCAATGTTATGGA+TGG	+	chr5.4:43407218-43407237	MS.gene59683:CDS	35.0%
!	AGTCAACCATATACCTTTTG+TGG	+	chr5.4:43407532-43407551	MS.gene59683:CDS	35.0%
!	CCTTTGAGTTTGCAATGTTA+TGG	+	chr5.4:43407214-43407233	MS.gene59683:CDS	35.0%
!!	AGTATCGATGGATACAACAT+CGG	+	chr5.4:43407610-43407629	MS.gene59683:CDS	35.0%
	ACCCTAACATTGAGTTCCAT+TGG	-	chr5.4:43407731-43407750	None:intergenic	40.0%
	CACAGTACCTTGCAATAATC+CGG	-	chr5.4:43406981-43407000	None:intergenic	40.0%
	CCGAGGTATGTTGATGTTAT+TGG	+	chr5.4:43407165-43407184	MS.gene59683:CDS	40.0%
	CTAACATTGAGTTCCATTGG+AGG	-	chr5.4:43407728-43407747	None:intergenic	40.0%
	CTCCAATGGAACTCAATGTT+AGG	+	chr5.4:43407726-43407745	MS.gene59683:CDS	40.0%
	GAAGCAATTTACATAGGGAG+TGG	+	chr5.4:43407635-43407654	MS.gene59683:CDS	40.0%
	GTATCCATCGATACTTTGCA+TGG	-	chr5.4:43407605-43407624	None:intergenic	40.0%
	GTGTACTAACCAAGTCACTT+TGG	-	chr5.4:43407389-43407408	None:intergenic	40.0%
!	ACTGGTGACATGCTTTTCTT+TGG	+	chr5.4:43407692-43407711	MS.gene59683:CDS	40.0%
!	ATGCGAATTGTTGATGGAGA+AGG	+	chr5.4:43407060-43407079	MS.gene59683:CDS	40.0%
!	TGACATGCTTTTCTTTGGAC+TGG	+	chr5.4:43407697-43407716	MS.gene59683:CDS	40.0%
!	TGCGAATTGTTGATGGAGAA+GGG	+	chr5.4:43407061-43407080	MS.gene59683:CDS	40.0%
!!	ATTCCCATGCAAAGTATCGA+TGG	+	chr5.4:43407598-43407617	MS.gene59683:CDS	40.0%
!!!	TTGCTGCTTTTGACTTTCAG+TGG	+	chr5.4:43407331-43407350	MS.gene59683:CDS	40.0%
!!!	TTTTGACTTTCAGTGGACCT+TGG	+	chr5.4:43407338-43407357	MS.gene59683:CDS	40.0%
	ACATTGCAAACTCAAAGGGG+AGG	-	chr5.4:43407212-43407231	None:intergenic	45.0%
	ATGTTATGGTCGGCCACAAA+AGG	-	chr5.4:43407548-43407567	None:intergenic	45.0%
	CAAACCTTCAAGTCAATCCG+AGG	+	chr5.4:43407148-43407167	MS.gene59683:CDS	45.0%
	CAATTTACATAGGGAGTGGC+TGG	+	chr5.4:43407639-43407658	MS.gene59683:CDS	45.0%
	TGGTTTCAATGAACCGCAGA+AGG	+	chr5.4:43406999-43407018	MS.gene59683:CDS	45.0%
!	GGCCGACCATAACATAGTTT+TGG	+	chr5.4:43407553-43407572	MS.gene59683:CDS	45.0%
!	GGGAACGACGTACCATATTT+TGG	+	chr5.4:43407081-43407100	MS.gene59683:CDS	45.0%
!!	CATACCTCGGATTGACTTGA+AGG	-	chr5.4:43407155-43407174	None:intergenic	45.0%
!!	CGGATTATTGCAAGGTACTG+TGG	+	chr5.4:43406979-43406998	MS.gene59683:CDS	45.0%
!!	CTCGGATTGACTTGAAGGTT+TGG	-	chr5.4:43407150-43407169	None:intergenic	45.0%
!!!	ATATAAAAAATAGATTTTAA+TGG	-	chr5.4:43407413-43407432	None:intergenic	5.0%
	AGTGGACCTTGGATGTAACC+AGG	+	chr5.4:43407349-43407368	MS.gene59683:CDS	50.0%
	GATGTTATTGGTCCCCCTGA+CGG	+	chr5.4:43407177-43407196	MS.gene59683:CDS	50.0%
	GTAACCAGGCCAGTTGCTAA+GGG	+	chr5.4:43407363-43407382	MS.gene59683:CDS	50.0%
	TCACTTTGGCCCTTAGCAAC+TGG	-	chr5.4:43407375-43407394	None:intergenic	50.0%
	TGCTAAGGGCCAAAGTGACT+TGG	+	chr5.4:43407377-43407396	MS.gene59683:CDS	50.0%
	TGTAACCAGGCCAGTTGCTA+AGG	+	chr5.4:43407362-43407381	MS.gene59683:CDS	50.0%
!	AACTGGCCTGGTTACATCCA+AGG	-	chr5.4:43407358-43407377	None:intergenic	50.0%
!	GGTCGGCCACAAAAGGTATA+TGG	-	chr5.4:43407541-43407560	None:intergenic	50.0%
!	TGGACTGGACAATCCTCCAA+TGG	+	chr5.4:43407712-43407731	MS.gene59683:CDS	50.0%
	AGGAAAAGACCACCGTCAGG+GGG	-	chr5.4:43407192-43407211	None:intergenic	55.0%
	ATGTTAGGGTTCTCCGTCGC+AGG	+	chr5.4:43407741-43407760	MS.gene59683:CDS	55.0%
	GAGGAAAAGACCACCGTCAG+GGG	-	chr5.4:43407193-43407212	None:intergenic	55.0%
	GGAGGAAAAGACCACCGTCA+GGG	-	chr5.4:43407194-43407213	None:intergenic	55.0%
	GAGGCGGCCGGATTATTGCA+AGG	+	chr5.4:43406971-43406990	MS.gene59683:CDS	60.0%
	GGGAGGAAAAGACCACCGTC+AGG	-	chr5.4:43407195-43407214	None:intergenic	60.0%
	GTTATTGGTCCCCCTGACGG+TGG	+	chr5.4:43407180-43407199	MS.gene59683:CDS	60.0%
	TTGGCCCTTAGCAACTGGCC+TGG	-	chr5.4:43407370-43407389	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	43406961	43407772	43406961	ID=MS.gene59683
chr5.4	mRNA	43406961	43407772	43406961	ID=MS.gene59683.t1;Parent=MS.gene59683
chr5.4	exon	43406961	43407398	43406961	ID=MS.gene59683.t1.exon1;Parent=MS.gene59683.t1
chr5.4	CDS	43406961	43407398	43406961	ID=cds.MS.gene59683.t1;Parent=MS.gene59683.t1
chr5.4	exon	43407515	43407772	43407515	ID=MS.gene59683.t1.exon2;Parent=MS.gene59683.t1
chr5.4	CDS	43407515	43407772	43407515	ID=cds.MS.gene59683.t1;Parent=MS.gene59683.t1

Gene Sequence

>MS.gene59683

ATGTTCATCAGAGGCGGCCGGATTATTGCAAGGTACTGTGGTTTCAATGAACCGCAGAAGGTAATTCTTGACTACAAAGTTAATGATAACCAGTTCAATATGCGAATTGTTGATGGAGAAGGGAACGACGTACCATATTTTGGATTCCATTATCCTGAAAATATTCATGCTAAGATACTTGCTAATCCAAACCTTCAAGTCAATCCGAGGTATGTTGATGTTATTGGTCCCCCTGACGGTGGTCTTTTCCTCCCCTTTGAGTTTGCAATGTTATGGATGGAAGAACTTAACGGTGTCAATGACGACAGCCTTATCCAATTTCTACAATTATCAGATGAACATAATGAGACTTCGGAATCTGATGAAGATGTTGCTGCTTTTGACTTTCAGTGGACCTTGGATGTAACCAGGCCAGTTGCTAAGGGCCAAAGTGACTTGGTTAGTACACACCATTAAAATCTATTTTTTATATTTGAAGACTTATGTTGAATTATTTAAGTTTAATTTATATATAATTATCCTGCATATTTAAACAAACATATTTATTTTTGTAGTATTTCCCCAAATCTGTAGTCAACCATATACCTTTTGTGGCCGACCATAACATAGTTTTGGTTGATGACAACACAAATGAGCGATTCCCATGCAAAGTATCGATGGATACAACATCGGAAGAAGCAATTTACATAGGGAGTGGCTGGTATGAATGTGCCAAAAGTCTAAGATTGAAGACTGGTGACATGCTTTTCTTTGGACTGGACAATCCTCCAATGGAACTCAATGTTAGGGTTCTCCGTCGCAGGTCTCACTGA

Protein sequence

>MS.gene59683.t1

MFIRGGRIIARYCGFNEPQKVILDYKVNDNQFNMRIVDGEGNDVPYFGFHYPENIHAKILANPNLQVNPRYVDVIGPPDGGLFLPFEFAMLWMEELNGVNDDSLIQFLQLSDEHNETSESDEDVAAFDFQWTLDVTRPVAKGQSDLYFPKSVVNHIPFVADHNIVLVDDNTNERFPCKVSMDTTSEEAIYIGSGWYECAKSLRLKTGDMLFFGLDNPPMELNVRVLRRRSH