AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59699

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59699.t1 MTR_4g021790 68.912 193 59 1 1 193 71 262 2.31e-89 265
MS.gene59699.t1 MTR_5g055260 68.868 212 36 5 1 210 71 254 1.20e-85 255
MS.gene59699.t1 MTR_4g021855 58.190 232 87 2 1 223 71 301 8.69e-85 254
MS.gene59699.t1 MTR_4g021760 57.143 231 97 2 1 230 72 301 3.18e-83 251
MS.gene59699.t1 MTR_4g021845 63.514 148 53 1 1 148 71 217 6.50e-60 188
MS.gene59699.t1 MTR_4g023980 63.115 122 44 1 1 122 51 171 1.07e-50 163
MS.gene59699.t1 MTR_1g032570 41.711 187 76 3 1 156 4 188 3.05e-38 140
MS.gene59699.t1 MTR_1g032570 41.711 187 76 3 1 156 73 257 4.90e-38 140
MS.gene59699.t1 MTR_2g034960 41.341 179 81 2 1 156 91 268 1.45e-36 136
MS.gene59699.t1 MTR_4g121020 41.758 182 78 4 1 156 93 272 1.42e-33 128
MS.gene59699.t1 MTR_4g121020 41.758 182 78 4 1 156 93 272 1.50e-33 128
MS.gene59699.t1 MTR_6g045327 42.778 180 75 5 1 167 76 240 1.77e-32 122
MS.gene59699.t1 MTR_4g131600 38.728 173 83 3 1 151 82 253 2.61e-30 118
MS.gene59699.t1 MTR_3g106220 38.503 187 83 4 1 156 70 255 3.94e-30 118
MS.gene59699.t1 MTR_3g086100 37.158 183 87 4 1 156 4 185 4.21e-30 117
MS.gene59699.t1 MTR_2g450070 37.113 194 83 5 1 157 80 271 4.76e-30 118
MS.gene59699.t1 MTR_2g450070 37.113 194 83 5 1 157 80 271 4.93e-30 118
MS.gene59699.t1 MTR_3g086100 37.158 183 87 4 1 156 75 256 5.24e-30 117
MS.gene59699.t1 MTR_8g105600 39.429 175 87 1 1 156 70 244 7.73e-30 117
MS.gene59699.t1 MTR_8g079940 35.359 181 91 3 1 156 87 266 7.62e-29 114
MS.gene59699.t1 MTR_1g013170 35.326 184 89 4 1 156 67 248 2.40e-27 109
MS.gene59699.t1 MTR_4g121020 42.424 165 67 4 18 156 6 168 5.23e-27 108
MS.gene59699.t1 MTR_4g131580 36.022 186 94 3 1 162 79 263 5.37e-27 108
MS.gene59699.t1 MTR_4g131570 36.612 183 88 4 1 156 79 260 6.24e-27 108
MS.gene59699.t1 MTR_1g013180 36.413 184 87 5 1 156 218 399 3.18e-25 103
MS.gene59699.t1 MTR_7g117705 33.516 182 92 4 1 155 68 247 2.20e-24 100
MS.gene59699.t1 MTR_7g026400 36.813 182 82 6 1 161 49 218 5.84e-24 100
MS.gene59699.t1 MTR_3g102600 32.692 156 69 2 1 156 21 140 2.64e-22 90.5
MS.gene59699.t1 MTR_3g102600 32.692 156 69 2 1 156 70 189 6.09e-22 90.9
MS.gene59699.t1 MTR_3g102590 35.211 142 65 3 17 156 86 202 6.72e-21 88.2
MS.gene59699.t1 MTR_0450s0040 33.918 171 97 4 1 161 66 230 9.69e-20 87.0
MS.gene59699.t1 MTR_8g093040 37.500 160 83 4 1 160 76 218 1.08e-19 85.9
MS.gene59699.t1 MTR_6g016850 45.055 91 49 1 1 90 70 160 8.97e-19 80.9
MS.gene59699.t1 MTR_3g102590 34.091 132 62 2 17 148 86 192 2.99e-18 80.1
MS.gene59699.t1 MTR_7g098250 48.101 79 40 1 90 167 253 331 3.13e-16 77.8
MS.gene59699.t1 MTR_5g041350 60.345 58 22 1 95 151 11 68 3.79e-16 77.0
MS.gene59699.t1 MTR_2g086450 41.748 103 49 3 55 151 1 98 5.26e-16 76.6
MS.gene59699.t1 MTR_1g013160 29.333 150 76 4 1 122 1 148 1.07e-15 72.4
MS.gene59699.t1 MTR_6g032990 56.061 66 26 2 95 159 38 101 1.39e-15 75.5
MS.gene59699.t1 MTR_2g016220 38.835 103 61 2 97 198 87 188 1.93e-15 75.1
MS.gene59699.t1 MTR_4g064730 49.315 73 36 1 85 156 116 188 1.95e-15 74.7
MS.gene59699.t1 MTR_2g016220 38.835 103 61 2 97 198 87 188 2.35e-15 74.7
MS.gene59699.t1 MTR_7g089010 57.627 59 25 0 98 156 90 148 3.74e-15 73.6
MS.gene59699.t1 MTR_7g115530 50.000 70 34 1 99 167 218 287 6.48e-15 72.8
MS.gene59699.t1 MTR_1g080330 44.828 87 45 2 95 180 171 255 1.05e-14 72.0
MS.gene59699.t1 MTR_7g115530 50.000 70 34 1 99 167 218 287 1.11e-14 72.4
MS.gene59699.t1 MTR_7g115530 50.000 70 34 1 99 167 218 287 1.24e-14 72.8
MS.gene59699.t1 MTR_1g080330 46.988 83 41 2 99 180 175 255 1.87e-14 72.4
MS.gene59699.t1 MTR_2g016220 38.318 107 60 4 97 198 87 192 2.04e-14 72.0
MS.gene59699.t1 MTR_7g115530 60.000 55 21 1 99 152 218 272 3.03e-14 71.6
MS.gene59699.t1 MTR_3g450310 48.052 77 33 2 89 158 3 79 5.04e-14 69.3
MS.gene59699.t1 MTR_7g069660 55.357 56 24 1 97 151 69 124 6.10e-14 70.5
MS.gene59699.t1 MTR_0223s0040 52.632 57 26 1 97 152 16 72 7.43e-14 69.7
MS.gene59699.t1 MTR_4g081710 55.172 58 25 1 95 151 13 70 2.79e-13 68.6
MS.gene59699.t1 MTR_2g023580 52.632 57 26 1 97 152 53 109 3.79e-13 67.0
MS.gene59699.t1 MTR_1g112370 52.632 57 26 1 97 152 20 76 5.53e-13 67.8
MS.gene59699.t1 MTR_2g090675 41.250 80 47 0 76 155 138 217 6.52e-13 67.8
MS.gene59699.t1 MTR_8g086410 51.786 56 26 1 97 151 107 162 8.05e-13 67.8
MS.gene59699.t1 MTR_8g086410 51.786 56 26 1 97 151 109 164 8.08e-13 67.8
MS.gene59699.t1 MTR_7g115530 59.615 52 20 1 99 149 218 269 1.04e-12 66.2
MS.gene59699.t1 MTR_6g444980 50.000 66 32 1 87 151 23 88 1.32e-12 67.0
MS.gene59699.t1 MTR_6g444980 50.000 66 32 1 87 151 23 88 1.58e-12 66.6
MS.gene59699.t1 MTR_7g088070 36.029 136 75 4 42 174 2 128 1.77e-12 65.9
MS.gene59699.t1 MTR_7g093030 53.571 56 25 1 97 151 210 265 1.90e-12 65.9
MS.gene59699.t1 MTR_7g093030 53.571 56 25 1 97 151 210 265 1.99e-12 66.2
MS.gene59699.t1 MTR_1g090670 57.407 54 22 1 99 151 44 97 2.15e-12 65.9
MS.gene59699.t1 MTR_8g086410 44.928 69 33 2 97 160 109 177 2.57e-12 66.2
MS.gene59699.t1 MTR_5g027440 57.407 54 22 1 99 151 25 78 2.89e-12 65.1
MS.gene59699.t1 MTR_4g098870 49.275 69 34 1 94 162 325 392 3.07e-12 66.2
MS.gene59699.t1 MTR_2g027800 35.772 123 70 4 99 212 39 161 3.07e-12 65.5
MS.gene59699.t1 MTR_4g098870 49.275 69 34 1 94 162 305 372 3.23e-12 65.9
MS.gene59699.t1 MTR_4g078810 51.724 58 28 0 98 155 157 214 3.35e-12 65.9
MS.gene59699.t1 MTR_7g088070 37.398 123 65 4 42 161 2 115 3.35e-12 65.1
MS.gene59699.t1 MTR_4g098870 48.611 72 36 1 95 166 326 396 4.16e-12 65.9
MS.gene59699.t1 MTR_1g053835 44.444 81 40 3 90 169 251 327 4.22e-12 65.5
MS.gene59699.t1 MTR_4g098870 48.611 72 36 1 95 166 306 376 4.43e-12 65.5
MS.gene59699.t1 MTR_4g098870 49.275 69 34 1 94 162 344 411 4.58e-12 65.5
MS.gene59699.t1 MTR_7g115530 56.604 53 22 1 99 150 218 270 5.55e-12 64.7
MS.gene59699.t1 MTR_2g084230 49.123 57 28 1 97 152 95 151 6.74e-12 64.7
MS.gene59699.t1 MTR_2g084230 49.123 57 28 1 97 152 95 151 6.90e-12 64.3
MS.gene59699.t1 MTR_2g027800 55.556 54 23 1 99 151 39 92 7.95e-12 64.3
MS.gene59699.t1 MTR_2g027860 44.944 89 39 3 99 177 49 137 9.35e-12 64.3
MS.gene59699.t1 MTR_5g014040 45.070 71 39 0 88 158 300 370 1.01e-11 64.7
MS.gene59699.t1 MTR_1g053800 56.140 57 24 1 99 154 177 233 1.04e-11 63.9
MS.gene59699.t1 MTR_1g053830 51.923 52 24 1 101 151 20 71 2.50e-11 59.3
MS.gene59699.t1 MTR_5g017980 49.180 61 31 0 96 156 189 249 8.01e-11 61.2
MS.gene59699.t1 MTR_5g017980 49.180 61 31 0 96 156 190 250 8.35e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59699.t1 AT3G16857 37.313 201 80 3 1 156 25 224 1.56e-33 127
MS.gene59699.t1 AT3G16857 37.313 201 80 3 1 156 95 294 2.41e-33 127
MS.gene59699.t1 AT3G16857 37.313 201 80 3 1 156 95 294 2.59e-33 127
MS.gene59699.t1 AT2G01760 37.895 190 83 4 1 156 4 192 1.20e-30 115
MS.gene59699.t1 AT2G01760 37.895 190 83 4 1 156 69 257 1.54e-30 117
MS.gene59699.t1 AT2G01760 37.895 190 83 4 1 156 97 285 2.80e-30 116
MS.gene59699.t1 AT5G58080 38.251 183 88 4 2 162 77 256 5.72e-30 117
MS.gene59699.t1 AT1G67710 36.765 204 98 5 1 174 4 206 5.09e-28 111
MS.gene59699.t1 AT1G67710 36.765 204 98 5 1 174 69 271 6.07e-28 111
MS.gene59699.t1 AT2G25180 36.872 179 89 3 1 156 75 252 6.32e-28 111
MS.gene59699.t1 AT3G62670 39.521 167 78 6 13 157 109 274 4.61e-26 105
MS.gene59699.t1 AT4G31920 36.047 172 96 4 1 160 75 244 4.97e-25 103
MS.gene59699.t1 AT1G67710 36.364 187 88 5 18 174 12 197 4.00e-21 91.7
MS.gene59699.t1 AT5G59570 49.315 73 34 1 87 156 126 198 1.89e-16 77.4
MS.gene59699.t1 AT5G59570 49.315 73 34 1 87 156 126 198 1.89e-16 77.4
MS.gene59699.t1 AT5G49240 30.636 173 93 4 2 148 101 272 2.62e-16 76.6
MS.gene59699.t1 AT3G04030 57.812 64 26 1 89 151 35 98 6.28e-16 76.6
MS.gene59699.t1 AT3G04030 57.812 64 26 1 89 151 35 98 6.38e-16 76.6
MS.gene59699.t1 AT3G46640 49.315 73 34 1 87 156 129 201 1.00e-15 75.5
MS.gene59699.t1 AT2G40970 50.746 67 33 0 90 156 96 162 1.11e-15 74.3
MS.gene59699.t1 AT5G18240 45.263 95 45 3 89 182 35 123 1.18e-15 75.9
MS.gene59699.t1 AT5G18240 45.263 95 45 3 89 182 35 123 1.18e-15 75.9
MS.gene59699.t1 AT5G05090 51.429 70 33 1 88 156 69 138 1.24e-15 74.7
MS.gene59699.t1 AT3G46640 49.315 73 34 1 87 156 129 201 1.25e-15 75.1
MS.gene59699.t1 AT3G46640 49.315 73 34 1 87 156 129 201 1.25e-15 75.1
MS.gene59699.t1 AT5G18240 45.263 95 45 3 89 182 35 123 1.39e-15 75.5
MS.gene59699.t1 AT5G18240 45.263 95 45 3 89 182 35 123 1.44e-15 75.5
MS.gene59699.t1 AT5G18240 45.263 95 45 3 89 182 35 123 1.44e-15 75.5
MS.gene59699.t1 AT3G10760 57.627 59 25 0 98 156 104 162 2.24e-15 74.7
MS.gene59699.t1 AT2G01060 51.765 85 37 3 88 168 4 88 2.41e-15 73.9
MS.gene59699.t1 AT3G04030 53.968 63 29 0 89 151 35 97 6.72e-15 73.6
MS.gene59699.t1 AT4G04605 44.156 77 41 2 97 171 12 88 2.00e-14 70.5
MS.gene59699.t1 AT5G06800 39.850 133 67 6 91 214 183 311 2.56e-14 72.0
MS.gene59699.t1 AT4G13640 40.385 104 52 3 99 198 37 134 2.96e-14 70.9
MS.gene59699.t1 AT4G13640 40.385 104 52 3 99 198 37 134 2.98e-14 71.2
MS.gene59699.t1 AT5G06800 39.130 138 66 6 91 214 183 316 7.25e-14 70.5
MS.gene59699.t1 AT5G44190 55.172 58 26 0 98 155 146 203 8.95e-14 70.5
MS.gene59699.t1 AT5G44190 55.172 58 26 0 98 155 146 203 1.20e-13 69.7
MS.gene59699.t1 AT3G24120 38.542 96 51 2 99 186 41 136 1.60e-13 68.9
MS.gene59699.t1 AT3G24120 38.542 96 51 2 99 186 41 136 1.65e-13 68.9
MS.gene59699.t1 AT1G69580 37.302 126 68 5 95 214 30 150 1.85e-13 69.3
MS.gene59699.t1 AT2G40260 57.143 56 23 1 97 151 80 135 1.97e-13 69.3
MS.gene59699.t1 AT2G20570 51.562 64 31 0 92 155 146 209 2.77e-13 68.9
MS.gene59699.t1 AT5G07210 40.196 102 54 1 62 156 186 287 3.41e-13 68.9
MS.gene59699.t1 AT5G07210 40.404 99 52 1 65 156 181 279 4.18e-13 68.9
MS.gene59699.t1 AT2G42660 55.556 54 23 1 100 152 51 104 4.27e-13 67.4
MS.gene59699.t1 AT1G14600 58.491 53 21 1 100 151 24 76 4.57e-13 67.4
MS.gene59699.t1 AT1G69580 39.252 107 58 4 95 199 30 131 4.67e-13 68.2
MS.gene59699.t1 AT5G29000 37.615 109 60 2 59 166 198 299 4.72e-13 67.8
MS.gene59699.t1 AT5G45580 52.308 65 30 1 88 151 12 76 5.75e-13 67.4
MS.gene59699.t1 AT5G29000 36.607 112 63 2 59 169 198 302 5.81e-13 68.2
MS.gene59699.t1 AT5G29000 36.607 112 63 2 59 169 155 259 6.07e-13 67.8
MS.gene59699.t1 AT5G29000 36.607 112 63 2 59 169 155 259 6.07e-13 67.8
MS.gene59699.t1 AT2G20570 56.364 55 24 0 101 155 171 225 6.41e-13 68.2
MS.gene59699.t1 AT5G06800 53.968 63 27 2 91 151 183 245 6.79e-13 67.0
MS.gene59699.t1 AT5G45580 59.259 54 21 1 99 151 7 60 1.29e-12 66.2
MS.gene59699.t1 AT1G79430 42.268 97 52 3 87 182 22 115 1.80e-12 66.2
MS.gene59699.t1 AT2G38300 50.877 57 27 1 97 152 52 108 1.86e-12 66.2
MS.gene59699.t1 AT4G28610 45.946 74 38 2 99 171 225 297 1.98e-12 66.6
MS.gene59699.t1 AT3G04450 52.542 59 27 1 95 152 234 292 2.67e-12 66.2
MS.gene59699.t1 AT3G04450 52.542 59 27 1 95 152 194 252 2.81e-12 65.9
MS.gene59699.t1 AT3G04450 52.542 59 27 1 95 152 194 252 2.81e-12 65.9
MS.gene59699.t1 AT2G02060 48.387 62 31 1 100 160 30 91 3.15e-12 65.1
MS.gene59699.t1 AT2G02060 48.387 62 31 1 100 160 30 91 3.15e-12 65.1
MS.gene59699.t1 AT3G04450 52.542 59 27 1 95 152 234 292 3.30e-12 65.5
MS.gene59699.t1 AT2G20400 40.217 92 52 2 99 189 231 320 3.73e-12 65.5
MS.gene59699.t1 AT2G20400 57.407 54 22 1 99 151 231 284 4.14e-12 65.5
MS.gene59699.t1 AT3G13040 36.752 117 64 5 98 211 240 349 4.31e-12 65.5
MS.gene59699.t1 AT3G13040 36.752 117 64 5 98 211 240 349 4.31e-12 65.5
MS.gene59699.t1 AT3G13040 36.752 117 64 5 98 211 240 349 4.31e-12 65.5

Find 44 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACAAAATTGGATGGAAAT+TGG 0.274934 5.4:-43619689 MS.gene59699:CDS
CATATGGGGCTTGCGAATAT+TGG 0.282422 5.4:-43620411 MS.gene59699:CDS
ACATGTTGCTAGTCATTTAC+AGG 0.286115 5.4:-43620006 MS.gene59699:intron
AATTCATCAGTTGATTATTT+CGG 0.292540 5.4:-43619599 MS.gene59699:CDS
AAGATGATGCACAACAAAAT+TGG 0.295199 5.4:-43619700 MS.gene59699:CDS
GAATTAATCACGACACAATC+TGG 0.322128 5.4:+43619617 None:intergenic
GAACGTCATCCTGTTGGTTC+CGG 0.354086 5.4:+43619765 None:intergenic
GTTACAAAGTCTATCACATA+TGG 0.379871 5.4:-43620427 MS.gene59699:CDS
TCTTCAAAATATTGGAAGCT+TGG 0.394226 5.4:-43620311 MS.gene59699:CDS
CGGGAAAGAGAAGATGATAA+TGG 0.414808 5.4:-43620268 MS.gene59699:CDS
CCCGCGGATAACTTTCATCT+TGG 0.418067 5.4:+43619726 None:intergenic
CTCAAGCTCAGCGGATTGAC+CGG 0.449486 5.4:-43619784 MS.gene59699:CDS
GCGGATTGACCGGAACCAAC+AGG 0.466931 5.4:-43619774 MS.gene59699:CDS
ATTGGAAGCTTGGATGTTCA+AGG 0.468841 5.4:-43620301 MS.gene59699:CDS
ATGTTCAAGGTCATAGAAAA+CGG 0.468876 5.4:-43620288 MS.gene59699:CDS
TTACAAAGTCTATCACATAT+GGG 0.475108 5.4:-43620426 MS.gene59699:CDS
TGGCATCATGTTGTAAGAAA+AGG 0.476984 5.4:-43620352 MS.gene59699:CDS
TTGGACCAAGCCGTTGTATG+AGG 0.493917 5.4:-43620392 MS.gene59699:CDS
GCCTCGTTTGTCATGGTCAC+CGG 0.494740 5.4:-43620221 MS.gene59699:CDS
GATCTTCATGGTTTGAGTGT+AGG 0.500247 5.4:-43620029 MS.gene59699:CDS
TGTTCAAGGTCATAGAAAAC+GGG 0.501689 5.4:-43620287 MS.gene59699:CDS
AGCATGTTGAACAAATTCAT+AGG 0.506691 5.4:+43620613 None:intergenic
ACACAATCTGGGAACAAGTT+GGG 0.508030 5.4:+43619629 None:intergenic
TTATGGGCTGTCAATCAACT+AGG 0.526348 5.4:-43620181 MS.gene59699:CDS
TTGAATTGTTCCTCATACAA+CGG 0.530062 5.4:+43620382 None:intergenic
AGGAAAGAACCATACTATCA+AGG 0.531753 5.4:+43620158 None:intergenic
GACACAATCTGGGAACAAGT+TGG 0.557153 5.4:+43619628 None:intergenic
TGATGCACAACAAAATTGGA+TGG 0.559172 5.4:-43619696 MS.gene59699:CDS
AATTAATCACGACACAATCT+GGG 0.566059 5.4:+43619618 None:intergenic
TTTGTAACAGAACTTAATGT+GGG 0.567790 5.4:+43620442 None:intergenic
CATCTTGAACGTCATCCTGT+TGG 0.572615 5.4:+43619759 None:intergenic
CGAAGAAGCCTCGTTTGTCA+TGG 0.575576 5.4:-43620228 MS.gene59699:CDS
AACAGGAATATTGAGTTGTA+CGG 0.581006 5.4:+43620589 None:intergenic
CTTTGTAACAGAACTTAATG+TGG 0.582713 5.4:+43620441 None:intergenic
TTGTTCCTCATACAACGGCT+TGG 0.596667 5.4:+43620387 None:intergenic
AGGATGACGTTCAAGATGAG+TGG 0.604590 5.4:-43619754 MS.gene59699:CDS
ATCAGTTGATTATTTCGGCT+AGG 0.608424 5.4:-43619594 None:intergenic
CCAAGATGAAAGTTATCCGC+GGG 0.608633 5.4:-43619726 MS.gene59699:CDS
AGGAACAATTCAAGATGATG+TGG 0.609752 5.4:-43620372 MS.gene59699:CDS
TGTGCATCATCTTGTACCCG+CGG 0.630231 5.4:+43619710 None:intergenic
TACAAAGTCTATCACATATG+GGG 0.656295 5.4:-43620425 MS.gene59699:CDS
ACCAAGATGAAAGTTATCCG+CGG 0.659940 5.4:-43619727 MS.gene59699:CDS
TCCGGTGACCATGACAAACG+AGG 0.694527 5.4:+43620220 None:intergenic
GTGATGACTCTCAAGCTCAG+CGG 0.726092 5.4:-43619793 MS.gene59699:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATTTAAATTTAAATTTA+TGG - chr5.4:43620293-43620312 MS.gene59699:CDS 0.0%
!! AATAAAAATCTTCAAAATAT+TGG - chr5.4:43619896-43619915 MS.gene59699:intron 10.0%
!!! TTAAAATTGTAATTTTCATG+TGG - chr5.4:43620322-43620341 MS.gene59699:CDS 15.0%
!! AAGAAAAACTTACTAATAAC+AGG + chr5.4:43619646-43619665 None:intergenic 20.0%
!! AATTCATCAGTTGATTATTT+CGG - chr5.4:43620616-43620635 MS.gene59699:CDS 20.0%
!! ACAAAGCAAAGAAAATAATT+AGG + chr5.4:43620080-43620099 None:intergenic 20.0%
!! TAAAATGATTCTCAAAAAGA+TGG - chr5.4:43620164-43620183 MS.gene59699:intron 20.0%
!! TGTTTGTATTATTTAACAAC+TGG - chr5.4:43620097-43620116 MS.gene59699:intron 20.0%
!!! ATCTTTTTGAGAATCATTTT+AGG + chr5.4:43620165-43620184 None:intergenic 20.0%
! AAAAAAGTTGCGAATATTGT+CGG + chr5.4:43620557-43620576 None:intergenic 25.0%
! TTAAAATCACACAAAACATG+TGG + chr5.4:43620276-43620295 None:intergenic 25.0%
! TTACAAAGTCTATCACATAT+GGG - chr5.4:43619789-43619808 MS.gene59699:CDS 25.0%
! TTTGTAACAGAACTTAATGT+GGG + chr5.4:43619776-43619795 None:intergenic 25.0%
AAGATGATGCACAACAAAAT+TGG - chr5.4:43620515-43620534 MS.gene59699:intron 30.0%
AATTAATCACGACACAATCT+GGG + chr5.4:43620600-43620619 None:intergenic 30.0%
AGCATGTTGAACAAATTCAT+AGG + chr5.4:43619605-43619624 None:intergenic 30.0%
ATGTTCAAGGTCATAGAAAA+CGG - chr5.4:43619927-43619946 MS.gene59699:intron 30.0%
CAACAAAATTGGATGGAAAT+TGG - chr5.4:43620526-43620545 MS.gene59699:intron 30.0%
CGATTGATGAGAAAAAATGT+GGG - chr5.4:43620380-43620399 MS.gene59699:CDS 30.0%
CTTTGTAACAGAACTTAATG+TGG + chr5.4:43619777-43619796 None:intergenic 30.0%
GTTACAAAGTCTATCACATA+TGG - chr5.4:43619788-43619807 MS.gene59699:CDS 30.0%
TACAAAGTCTATCACATATG+GGG - chr5.4:43619790-43619809 MS.gene59699:CDS 30.0%
TCGATTGATGAGAAAAAATG+TGG - chr5.4:43620379-43620398 MS.gene59699:CDS 30.0%
TCTTCAAAATATTGGAAGCT+TGG - chr5.4:43619904-43619923 MS.gene59699:intron 30.0%
TTGAATTGTTCCTCATACAA+CGG + chr5.4:43619836-43619855 None:intergenic 30.0%
! AACAGGAATATTGAGTTGTA+CGG + chr5.4:43619629-43619648 None:intergenic 30.0%
! AACTGCATCAACGATTTTTA+TGG - chr5.4:43620017-43620036 MS.gene59699:CDS 30.0%
! ACTGCATCAACGATTTTTAT+GGG - chr5.4:43620018-43620037 MS.gene59699:CDS 30.0%
!! TTCTCTCACTTATTTTTCAG+AGG - chr5.4:43620134-43620153 MS.gene59699:intron 30.0%
AAAAATCGTTGATGCAGTTC+CGG + chr5.4:43620016-43620035 None:intergenic 35.0%
ACATGTTGCTAGTCATTTAC+AGG - chr5.4:43620209-43620228 MS.gene59699:CDS 35.0%
AGGAAAGAACCATACTATCA+AGG + chr5.4:43620060-43620079 None:intergenic 35.0%
AGGAACAATTCAAGATGATG+TGG - chr5.4:43619843-43619862 MS.gene59699:CDS 35.0%
ATTCAGTAGACATCACTACA+AGG + chr5.4:43619751-43619770 None:intergenic 35.0%
CTCAAAAAGATGGATCTTCA+TGG - chr5.4:43620174-43620193 MS.gene59699:CDS 35.0%
GAATTAATCACGACACAATC+TGG + chr5.4:43620601-43620620 None:intergenic 35.0%
TGATGCACAACAAAATTGGA+TGG - chr5.4:43620519-43620538 MS.gene59699:intron 35.0%
TGGCATCATGTTGTAAGAAA+AGG - chr5.4:43619863-43619882 MS.gene59699:CDS 35.0%
TGTTCAAGGTCATAGAAAAC+GGG - chr5.4:43619928-43619947 MS.gene59699:intron 35.0%
ACACAATCTGGGAACAAGTT+GGG + chr5.4:43620589-43620608 None:intergenic 40.0%
ACCAAGATGAAAGTTATCCG+CGG - chr5.4:43620488-43620507 MS.gene59699:intron 40.0%
ATTGGAAGCTTGGATGTTCA+AGG - chr5.4:43619914-43619933 MS.gene59699:intron 40.0%
CGGGAAAGAGAAGATGATAA+TGG - chr5.4:43619947-43619966 MS.gene59699:intron 40.0%
GATCTTCATGGTTTGAGTGT+AGG - chr5.4:43620186-43620205 MS.gene59699:CDS 40.0%
! TCAACTAGGCCTTGATAGTA+TGG - chr5.4:43620048-43620067 MS.gene59699:CDS 40.0%
! TTATGGGCTGTCAATCAACT+AGG - chr5.4:43620034-43620053 MS.gene59699:CDS 40.0%
AGGATGACGTTCAAGATGAG+TGG - chr5.4:43620461-43620480 MS.gene59699:CDS 45.0%
CATATGGGGCTTGCGAATAT+TGG - chr5.4:43619804-43619823 MS.gene59699:CDS 45.0%
CATCTTGAACGTCATCCTGT+TGG + chr5.4:43620459-43620478 None:intergenic 45.0%
CCAAGATGAAAGTTATCCGC+GGG - chr5.4:43620489-43620508 MS.gene59699:intron 45.0%
GACACAATCTGGGAACAAGT+TGG + chr5.4:43620590-43620609 None:intergenic 45.0%
TTGTTCCTCATACAACGGCT+TGG + chr5.4:43619831-43619850 None:intergenic 45.0%
CCCGCGGATAACTTTCATCT+TGG + chr5.4:43620492-43620511 None:intergenic 50.0%
CGAAGAAGCCTCGTTTGTCA+TGG - chr5.4:43619987-43620006 MS.gene59699:intron 50.0%
GTGATGACTCTCAAGCTCAG+CGG - chr5.4:43620422-43620441 MS.gene59699:CDS 50.0%
TGTGCATCATCTTGTACCCG+CGG + chr5.4:43620508-43620527 None:intergenic 50.0%
TTGGACCAAGCCGTTGTATG+AGG - chr5.4:43619823-43619842 MS.gene59699:CDS 50.0%
!! GAACGTCATCCTGTTGGTTC+CGG + chr5.4:43620453-43620472 None:intergenic 50.0%
GCCTCGTTTGTCATGGTCAC+CGG - chr5.4:43619994-43620013 MS.gene59699:intron 55.0%
TCCGGTGACCATGACAAACG+AGG + chr5.4:43619998-43620017 None:intergenic 55.0%
!! CTCAAGCTCAGCGGATTGAC+CGG - chr5.4:43620431-43620450 MS.gene59699:CDS 55.0%
! GCGGATTGACCGGAACCAAC+AGG - chr5.4:43620441-43620460 MS.gene59699:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 43619595 43620642 43619595 ID=MS.gene59699
chr5.4 mRNA 43619595 43620642 43619595 ID=MS.gene59699.t1;Parent=MS.gene59699
chr5.4 exon 43620585 43620642 43620585 ID=MS.gene59699.t1.exon1;Parent=MS.gene59699.t1
chr5.4 CDS 43620585 43620642 43620585 ID=cds.MS.gene59699.t1;Parent=MS.gene59699.t1
chr5.4 exon 43620173 43620481 43620173 ID=MS.gene59699.t1.exon2;Parent=MS.gene59699.t1
chr5.4 CDS 43620173 43620481 43620173 ID=cds.MS.gene59699.t1;Parent=MS.gene59699.t1
chr5.4 exon 43620007 43620083 43620007 ID=MS.gene59699.t1.exon3;Parent=MS.gene59699.t1
chr5.4 CDS 43620007 43620083 43620007 ID=cds.MS.gene59699.t1;Parent=MS.gene59699.t1
chr5.4 exon 43619595 43619882 43619595 ID=MS.gene59699.t1.exon4;Parent=MS.gene59699.t1
chr5.4 CDS 43619595 43619882 43619595 ID=cds.MS.gene59699.t1;Parent=MS.gene59699.t1
Gene Sequence

>MS.gene59699

ATGGATGCCTATGAATTTGTTCAACATGCTACCGTACAACTCAATATTCCTGTTATTAGTAAGTTTTTCTTCATCTTTATACTTATACTTTGTTACATTCATTCAAATATATATACTTATGAAGTGTGAGTGTGACTGACATTGATATTTCTTCCTTGTAGTGATGTCTACTGAATATCCCACATTAAGTTCTGTTACAAAGTCTATCACATATGGGGCTTGCGAATATTGGACCAAGCCGTTGTATGAGGAACAATTCAAGATGATGTGGCATCATGTTGTAAGAAAAGGCTTGAGCAAAAATAAAAATCTTCAAAATATTGGAAGCTTGGATGTTCAAGGTCATAGAAAACGGGAAAGAGAAGATGATAATGGATCAAAAGAATCAAATGCGAAGAAGCCTCGTTTGTCATGGTCACCGGAACTGCATCAACGATTTTTATGGGCTGTCAATCAACTAGGCCTTGATAGTATGGTTCTTTCCTAATTATTTTCTTTGCTTTGTTTGTATTATTTAACAACTGGACTAATGTGCATTTTTCTCTCACTTATTTTTCAGAGGCAAAACCTAAAATGATTCTCAAAAAGATGGATCTTCATGGTTTGAGTGTAGGACATGTTGCTAGTCATTTACAGGTTTGTTTTTTAATAGAATCTAAGAAAATTATTGATAATATACCACATGTTTTGTGTGATTTTAAATTTAAATTTAAATTTATGGAGTTTATTAAAATTGTAATTTTCATGTGGTAATTTGCAGAAATATAGAAACTATTTGAAGAGATCGATTGATGAGAAAAAATGTGGGAAGAAAAAATCAAAGCTGAGTGATGACTCTCAAGCTCAGCGGATTGACCGGAACCAACAGGATGACGTTCAAGATGAGTGGATTGACCAAGATGAAAGTTATCCGCGGGTACAAGATGATGCACAACAAAATTGGATGGAAATTGGCAAATCCGACAATATTCGCAACTTTTTTTCTGACATACCCAACTTGTTCCCAGATTGTGTCGTGATTAATTCATCAGTTGATTATTTCGGCTAG
Protein sequence

>MS.gene59699.t1

MDAYEFVQHATVQLNIPVIMMSTEYPTLSSVTKSITYGACEYWTKPLYEEQFKMMWHHVVRKGLSKNKNLQNIGSLDVQGHRKREREDDNGSKESNAKKPRLSWSPELHQRFLWAVNQLGLDKAKPKMILKKMDLHGLSVGHVASHLQKYRNYLKRSIDEKKCGKKKSKLSDDSQAQRIDRNQQDDVQDEWIDQDESYPRVQDDAQQNWMEIGKSDNIRNFFSDIPNLFPDCVVINSSVDYFG