Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59952.t1 | XP_024636802.1 | 70.4 | 233 | 68 | 1 | 1 | 233 | 1 | 232 | 2.80E-58 | 235.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59952.t1 | A0A396IJH9 | 70.4 | 233 | 68 | 1 | 1 | 233 | 1 | 232 | 2.0e-58 | 235.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene59952.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59952.t1 | MTR_3g084720 | 47.347 | 245 | 98 | 5 | 1 | 230 | 1 | 229 | 4.12e-59 | 186 |
| MS.gene59952.t1 | MTR_6g465230 | 63.934 | 122 | 40 | 2 | 1 | 119 | 98 | 218 | 1.33e-36 | 128 |
| MS.gene59952.t1 | MTR_7g010470 | 65.000 | 80 | 28 | 0 | 154 | 233 | 1 | 80 | 3.85e-29 | 105 |
| MS.gene59952.t1 | MTR_6g055180 | 55.556 | 90 | 33 | 2 | 1 | 86 | 1 | 87 | 6.75e-19 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59952.t1 | AT2G18490 | 63.158 | 57 | 21 | 0 | 121 | 177 | 155 | 211 | 9.90e-21 | 87.8 |
| MS.gene59952.t1 | AT2G18490 | 63.158 | 57 | 21 | 0 | 121 | 177 | 205 | 261 | 1.66e-20 | 88.2 |
| MS.gene59952.t1 | AT2G15740 | 59.701 | 67 | 23 | 1 | 120 | 186 | 229 | 291 | 4.91e-20 | 87.4 |
| MS.gene59952.t1 | AT5G22990 | 65.079 | 63 | 21 | 1 | 116 | 177 | 227 | 289 | 5.24e-20 | 87.0 |
| MS.gene59952.t1 | AT5G42640 | 42.478 | 113 | 47 | 4 | 77 | 177 | 179 | 285 | 1.24e-19 | 85.9 |
Find 47 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCATTGAGCTTTAGAATC+TGG | 0.282776 | 8.3:-12615959 | None:intergenic |
| GTTTATTGTGGGGTAGACTA+TGG | 0.293623 | 8.3:-12615791 | None:intergenic |
| CATCATCTGATAATAATAAT+AGG | 0.318777 | 8.3:+12615571 | MS.gene59952:CDS |
| AACAATATTGTTGCTACTAA+TGG | 0.346446 | 8.3:+12615696 | MS.gene59952:CDS |
| CAAATGCTTAAAACACAGTT+TGG | 0.350424 | 8.3:+12615528 | MS.gene59952:CDS |
| TACTAATGGTGATGAAGTTC+GGG | 0.365107 | 8.3:+12615710 | MS.gene59952:CDS |
| CTACTAATGGTGATGAAGTT+CGG | 0.369876 | 8.3:+12615709 | MS.gene59952:CDS |
| TCTGGAGATCTGGTCTCTTT+CGG | 0.392995 | 8.3:-12615941 | None:intergenic |
| AGCTTTAGAATCTGGAGATC+TGG | 0.424493 | 8.3:-12615951 | None:intergenic |
| TCAGCCGATAGTAACAATTT+CGG | 0.440639 | 8.3:+12615474 | MS.gene59952:CDS |
| TGTATTGATTTGATGATGCC+TGG | 0.446641 | 8.3:-12615507 | None:intergenic |
| CTATTATTATTATCAGATGA+TGG | 0.447972 | 8.3:-12615570 | None:intergenic |
| GCTATTACCTTGTTGCACTT+TGG | 0.453373 | 8.3:-12615831 | None:intergenic |
| TTGCACTTTGGACATATGTA+TGG | 0.455080 | 8.3:-12615819 | None:intergenic |
| GACGAGCCTCCAAGGAGATC+AGG | 0.458143 | 8.3:-12615651 | None:intergenic |
| CAACCACCATGAGTTCTGAT+AGG | 0.471836 | 8.3:+12615412 | None:intergenic |
| ACAATATTGTTTGCCTCAGA+TGG | 0.473153 | 8.3:-12615684 | None:intergenic |
| TACGCTCATCCTGATCTCCT+TGG | 0.478788 | 8.3:+12615642 | MS.gene59952:CDS |
| ACCCCACTTAGAGGAGGAGC+AGG | 0.481368 | 8.3:-12616068 | None:intergenic |
| TCCCTGCTCCTCCTCTAAGT+GGG | 0.487095 | 8.3:+12616066 | MS.gene59952:CDS |
| AAAGAAGAAATTGTTGATGA+TGG | 0.494936 | 8.3:+12615762 | MS.gene59952:CDS |
| GTCCCTGCTCCTCCTCTAAG+TGG | 0.497357 | 8.3:+12616065 | MS.gene59952:CDS |
| TGTATGGTCCATGTTTATTG+TGG | 0.503637 | 8.3:-12615803 | None:intergenic |
| GTTCTGATAGGTTGATGAAA+TGG | 0.508320 | 8.3:+12615424 | MS.gene59952:CDS |
| TATTGTTTGCCTCAGATGGC+GGG | 0.510054 | 8.3:-12615680 | None:intergenic |
| CAGATTCTAAAGCTCAATGA+TGG | 0.517574 | 8.3:+12615960 | MS.gene59952:CDS |
| AATATATATTAATTATCAGC+AGG | 0.528844 | 8.3:-12616107 | None:intergenic |
| GTATGGTCCATGTTTATTGT+GGG | 0.530171 | 8.3:-12615802 | None:intergenic |
| GCTCATCCTGATCTCCTTGG+AGG | 0.534371 | 8.3:+12615645 | MS.gene59952:CDS |
| CATCAACCTATCAGAACTCA+TGG | 0.543143 | 8.3:-12615418 | None:intergenic |
| CAAATAGTACCAACATGAAG+AGG | 0.557023 | 8.3:+12615736 | MS.gene59952:CDS |
| GGTGTGATGACGAGCCTCCA+AGG | 0.564529 | 8.3:-12615659 | None:intergenic |
| AATTTCGGTAGTCATGCTCC+AGG | 0.573474 | 8.3:+12615489 | MS.gene59952:CDS |
| ACTACCGAAATTGTTACTAT+CGG | 0.574375 | 8.3:-12615478 | None:intergenic |
| CCCTGCTCCTCCTCTAAGTG+GGG | 0.591604 | 8.3:+12616067 | MS.gene59952:CDS |
| ATATTGTTTGCCTCAGATGG+CGG | 0.593923 | 8.3:-12615681 | None:intergenic |
| TGAGTATCATAACTGACAGA+AGG | 0.596005 | 8.3:-12616032 | None:intergenic |
| GTCATCACACCCGCCATCTG+AGG | 0.607113 | 8.3:+12615671 | MS.gene59952:CDS |
| GTTACTATCGGCTGATGATG+TGG | 0.609878 | 8.3:-12615466 | None:intergenic |
| TATGGTCCATGTTTATTGTG+GGG | 0.620016 | 8.3:-12615801 | None:intergenic |
| CATATGTCCAAAGTGCAACA+AGG | 0.620267 | 8.3:+12615824 | MS.gene59952:CDS |
| TGTTATCAACAACAACAAGA+TGG | 0.620498 | 8.3:+12615599 | MS.gene59952:CDS |
| TGATCAGTAGAAGCAATGAC+AGG | 0.628764 | 8.3:-12615999 | None:intergenic |
| TGTATCAAGTCACTACAAGT+CGG | 0.647352 | 8.3:+12615878 | MS.gene59952:CDS |
| AGTCTACCCCACAATAAACA+TGG | 0.648429 | 8.3:+12615795 | MS.gene59952:CDS |
| CCCCACTTAGAGGAGGAGCA+GGG | 0.660323 | 8.3:-12616067 | None:intergenic |
| AGGTTGATGAAATGGTTGTG+TGG | 0.707031 | 8.3:+12615432 | MS.gene59952:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATCATCTGATAATAATAAT+AGG | + | chr8.3:12615571-12615590 | MS.gene59952:CDS | 20.0% |
| !! | CTATTATTATTATCAGATGA+TGG | - | chr8.3:12615573-12615592 | None:intergenic | 20.0% |
| ! | AACAATATTGTTGCTACTAA+TGG | + | chr8.3:12615696-12615715 | MS.gene59952:CDS | 25.0% |
| !! | AAAGAAGAAATTGTTGATGA+TGG | + | chr8.3:12615762-12615781 | MS.gene59952:CDS | 25.0% |
| ACTACCGAAATTGTTACTAT+CGG | - | chr8.3:12615481-12615500 | None:intergenic | 30.0% | |
| CAAATGCTTAAAACACAGTT+TGG | + | chr8.3:12615528-12615547 | MS.gene59952:CDS | 30.0% | |
| GAAAAAAGAGATACATGTCT+AGG | + | chr8.3:12615916-12615935 | MS.gene59952:CDS | 30.0% | |
| TGTTATCAACAACAACAAGA+TGG | + | chr8.3:12615599-12615618 | MS.gene59952:CDS | 30.0% | |
| !!! | TTCTTTTTTCCTCTTCATGT+TGG | - | chr8.3:12615748-12615767 | None:intergenic | 30.0% |
| ACAATATTGTTTGCCTCAGA+TGG | - | chr8.3:12615687-12615706 | None:intergenic | 35.0% | |
| CAAATAGTACCAACATGAAG+AGG | + | chr8.3:12615736-12615755 | MS.gene59952:CDS | 35.0% | |
| GTATGGTCCATGTTTATTGT+GGG | - | chr8.3:12615805-12615824 | None:intergenic | 35.0% | |
| TATGGTCCATGTTTATTGTG+GGG | - | chr8.3:12615804-12615823 | None:intergenic | 35.0% | |
| TCAGCCGATAGTAACAATTT+CGG | + | chr8.3:12615474-12615493 | MS.gene59952:CDS | 35.0% | |
| TGAGTATCATAACTGACAGA+AGG | - | chr8.3:12616035-12616054 | None:intergenic | 35.0% | |
| TGTATCAAGTCACTACAAGT+CGG | + | chr8.3:12615878-12615897 | MS.gene59952:CDS | 35.0% | |
| TGTATGGTCCATGTTTATTG+TGG | - | chr8.3:12615806-12615825 | None:intergenic | 35.0% | |
| TTGCACTTTGGACATATGTA+TGG | - | chr8.3:12615822-12615841 | None:intergenic | 35.0% | |
| ! | CTACTAATGGTGATGAAGTT+CGG | + | chr8.3:12615709-12615728 | MS.gene59952:CDS | 35.0% |
| ! | TACTAATGGTGATGAAGTTC+GGG | + | chr8.3:12615710-12615729 | MS.gene59952:CDS | 35.0% |
| ! | TTAACTTTTACCCCACTTAG+AGG | - | chr8.3:12616080-12616099 | None:intergenic | 35.0% |
| !! | CAGATTCTAAAGCTCAATGA+TGG | + | chr8.3:12615960-12615979 | MS.gene59952:CDS | 35.0% |
| !! | CATCATTGAGCTTTAGAATC+TGG | - | chr8.3:12615962-12615981 | None:intergenic | 35.0% |
| !! | GTTCTGATAGGTTGATGAAA+TGG | + | chr8.3:12615424-12615443 | MS.gene59952:CDS | 35.0% |
| !! | TGTATTGATTTGATGATGCC+TGG | - | chr8.3:12615510-12615529 | None:intergenic | 35.0% |
| AGTCTACCCCACAATAAACA+TGG | + | chr8.3:12615795-12615814 | MS.gene59952:CDS | 40.0% | |
| ATATTGTTTGCCTCAGATGG+CGG | - | chr8.3:12615684-12615703 | None:intergenic | 40.0% | |
| CATATGTCCAAAGTGCAACA+AGG | + | chr8.3:12615824-12615843 | MS.gene59952:CDS | 40.0% | |
| GCTATTACCTTGTTGCACTT+TGG | - | chr8.3:12615834-12615853 | None:intergenic | 40.0% | |
| GTTTATTGTGGGGTAGACTA+TGG | - | chr8.3:12615794-12615813 | None:intergenic | 40.0% | |
| TGATCAGTAGAAGCAATGAC+AGG | - | chr8.3:12616002-12616021 | None:intergenic | 40.0% | |
| !! | AGCTTTAGAATCTGGAGATC+TGG | - | chr8.3:12615954-12615973 | None:intergenic | 40.0% |
| !! | AGGTTGATGAAATGGTTGTG+TGG | + | chr8.3:12615432-12615451 | MS.gene59952:CDS | 40.0% |
| AATTTCGGTAGTCATGCTCC+AGG | + | chr8.3:12615489-12615508 | MS.gene59952:CDS | 45.0% | |
| TATTGTTTGCCTCAGATGGC+GGG | - | chr8.3:12615683-12615702 | None:intergenic | 45.0% | |
| TCTGGAGATCTGGTCTCTTT+CGG | - | chr8.3:12615944-12615963 | None:intergenic | 45.0% | |
| ! | ACTTTTACCCCACTTAGAGG+AGG | - | chr8.3:12616077-12616096 | None:intergenic | 45.0% |
| ! | GTTACTATCGGCTGATGATG+TGG | - | chr8.3:12615469-12615488 | None:intergenic | 45.0% |
| TACGCTCATCCTGATCTCCT+TGG | + | chr8.3:12615642-12615661 | MS.gene59952:CDS | 50.0% | |
| GCTCATCCTGATCTCCTTGG+AGG | + | chr8.3:12615645-12615664 | MS.gene59952:CDS | 55.0% | |
| TCCCTGCTCCTCCTCTAAGT+GGG | + | chr8.3:12616066-12616085 | MS.gene59952:CDS | 55.0% | |
| ACCCCACTTAGAGGAGGAGC+AGG | - | chr8.3:12616071-12616090 | None:intergenic | 60.0% | |
| CCCCACTTAGAGGAGGAGCA+GGG | - | chr8.3:12616070-12616089 | None:intergenic | 60.0% | |
| CCCTGCTCCTCCTCTAAGTG+GGG | + | chr8.3:12616067-12616086 | MS.gene59952:CDS | 60.0% | |
| GACGAGCCTCCAAGGAGATC+AGG | - | chr8.3:12615654-12615673 | None:intergenic | 60.0% | |
| GTCATCACACCCGCCATCTG+AGG | + | chr8.3:12615671-12615690 | MS.gene59952:CDS | 60.0% | |
| GTCCCTGCTCCTCCTCTAAG+TGG | + | chr8.3:12616065-12616084 | MS.gene59952:CDS | 60.0% | |
| ! | GGTGTGATGACGAGCCTCCA+AGG | - | chr8.3:12615662-12615681 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 12615420 | 12616121 | 12615420 | ID=MS.gene59952 |
| chr8.3 | mRNA | 12615420 | 12616121 | 12615420 | ID=MS.gene59952.t1;Parent=MS.gene59952 |
| chr8.3 | exon | 12615420 | 12616121 | 12615420 | ID=MS.gene59952.t1.exon1;Parent=MS.gene59952.t1 |
| chr8.3 | CDS | 12615420 | 12616121 | 12615420 | ID=cds.MS.gene59952.t1;Parent=MS.gene59952.t1 |
| Gene Sequence |
| Protein sequence |
