AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60182


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60182.t1 MTR_4g097440 95.402 174 8 0 1 174 1 174 4.04e-123 344
MS.gene60182.t1 MTR_5g015090 56.897 174 58 6 1 174 1 157 7.43e-58 178
MS.gene60182.t1 MTR_3g103360 47.619 84 44 0 71 154 52 135 2.68e-18 77.0
MS.gene60182.t1 MTR_4g070860 41.935 93 54 0 62 154 17 109 3.19e-17 74.7
MS.gene60182.t1 MTR_1g017570 65.152 66 23 0 74 139 59 124 5.28e-17 73.9
MS.gene60182.t1 MTR_1g022495 38.739 111 66 1 44 154 1 109 8.16e-16 71.2
MS.gene60182.t1 MTR_3g117120 41.053 95 49 1 67 154 15 109 2.58e-15 69.7
MS.gene60182.t1 MTR_7g029400 34.091 132 75 2 16 147 37 156 7.74e-15 69.3
MS.gene60182.t1 MTR_6g016375 43.182 88 50 0 63 150 78 165 1.82e-14 68.6
MS.gene60182.t1 MTR_8g015250 44.706 85 47 0 63 147 62 146 1.82e-14 68.2
MS.gene60182.t1 MTR_8g086020 38.554 83 51 0 72 154 32 114 3.23e-13 64.3
MS.gene60182.t1 MTR_7g017880 41.176 85 50 0 63 147 75 159 3.70e-13 64.7
MS.gene60182.t1 MTR_1g087040 33.333 96 61 1 72 164 24 119 6.42e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60182.t1 AT2G22850 35.047 214 85 5 1 160 1 214 1.85e-30 110
MS.gene60182.t1 AT2G22850 35.047 214 85 5 1 160 1 214 1.85e-30 110
MS.gene60182.t1 AT3G49760 37.654 162 90 4 1 158 1 155 8.53e-29 104
MS.gene60182.t1 AT4G37730 35.681 213 72 6 1 148 58 270 1.44e-25 100
MS.gene60182.t1 AT2G18160 45.783 83 45 0 72 154 28 110 5.00e-17 74.7
MS.gene60182.t1 AT5G38800 35.526 152 73 3 2 150 18 147 8.20e-17 73.9
MS.gene60182.t1 AT1G75390 40.860 93 55 0 62 154 28 120 5.30e-16 72.0
MS.gene60182.t1 AT3G30530 34.000 150 88 2 1 150 18 156 2.46e-15 70.1
MS.gene60182.t1 AT4G34590 38.372 86 53 0 69 154 21 106 1.08e-13 65.5
MS.gene60182.t1 AT1G75390 43.590 78 44 0 62 139 28 105 1.41e-13 64.3
MS.gene60182.t1 AT1G13600 40.217 92 48 1 63 154 74 158 3.28e-13 65.1
MS.gene60182.t1 AT5G15830 42.169 83 48 0 71 153 70 152 3.96e-13 64.7
MS.gene60182.t1 AT2G04038 43.299 97 44 2 64 157 60 148 4.24e-12 61.6
MS.gene60182.t1 AT3G62420 37.895 95 57 2 62 154 10 104 8.16e-12 60.5
MS.gene60182.t1 AT1G59530 37.241 145 82 3 29 172 10 146 3.39e-11 58.5
MS.gene60182.t1 AT5G49450 34.524 84 55 0 72 155 13 96 4.85e-11 58.2

Find 0 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GAAACAAAATGAATTGTAAC+CGG + 32965:29961-29980 None:intergenic 25.0%
! GTTTGATATCATCTGAAAAT+AGG + 32965:29700-29719 None:intergenic 25.0%
!! CAACAACTTTACTCAAATTT+TGG - 32965:30048-30067 MS.gene60182:CDS 25.0%
AAAGACATGTTGAGAATCTA+AGG - 32965:29891-29910 MS.gene60182:CDS 30.0%
AACTTGAAGTGATAGGTTTA+GGG + 32965:29730-29749 None:intergenic 30.0%
!! GAGTAAAGTTGTTGATTCTT+TGG + 32965:30042-30061 None:intergenic 30.0%
!! TCATTTTGTTTCACTTGAAC+CGG - 32965:29969-29988 MS.gene60182:CDS 30.0%
AAAACCGGGAAATGAAAAAC+CGG - 32965:29939-29958 MS.gene60182:CDS 35.0%
ACAAGTGTAGAATGGAAAAC+CGG - 32965:29924-29943 MS.gene60182:CDS 35.0%
ATGGACATGTAACACTACTA+TGG - 32965:30102-30121 MS.gene60182:CDS 35.0%
GAACTTGAAGTGATAGGTTT+AGG + 32965:29731-29750 None:intergenic 35.0%
TGGAGAAAGGTTGAAATTGT+TGG + 32965:30078-30097 None:intergenic 35.0%
! AAACCTATCACTTCAAGTTC+CGG - 32965:29731-29750 MS.gene60182:CDS 35.0%
!! ATTCATTTTCGGTTCGTATC+CGG + 32965:29991-30010 None:intergenic 35.0%
AATAGGTCGTGAAAATCCCA+CGG + 32965:29683-29702 None:intergenic 40.0%
ACCTCATGAGAAACGTAAAC+CGG - 32965:29772-29791 MS.gene60182:CDS 40.0%
AGTGTTACATGTCCATGAAG+TGG + 32965:30098-30117 None:intergenic 40.0%
CAAGTGTAGAATGGAAAACC+GGG - 32965:29925-29944 MS.gene60182:CDS 40.0%
CCAGTTGAACAAGTGTAGAA+TGG - 32965:29916-29935 MS.gene60182:CDS 40.0%
CCATTCTACACTTGTTCAAC+TGG + 32965:29919-29938 None:intergenic 40.0%
GAACCGAAAATGAATGGCTT+CGG - 32965:29996-30015 MS.gene60182:CDS 40.0%
GACATGTAACACTACTATGG+TGG - 32965:30105-30124 MS.gene60182:CDS 40.0%
GGATACGAACCGAAAATGAA+TGG - 32965:29990-30009 MS.gene60182:CDS 40.0%
GTTTACGTTTCTCATGAGGT+TGG + 32965:29772-29791 None:intergenic 40.0%
TTCAACCTTTCTCCACTTCA+TGG - 32965:30083-30102 MS.gene60182:CDS 40.0%
! AGACCGAAGCCATTCATTTT+CGG + 32965:30002-30021 None:intergenic 40.0%
! GTTGATTCTTTGGTTCAGCA+TGG + 32965:30032-30051 None:intergenic 40.0%
!! AGAATGGATTGTCGAGAAGA+GGG + 32965:29637-29656 None:intergenic 40.0%
!! GTAACCGGTTTTTCATTTCC+CGG + 32965:29946-29965 None:intergenic 40.0%
!! TTGGTTTGGTTCATCTGAAC+CGG + 32965:29753-29772 None:intergenic 40.0%
CATGTCCATGAAGTGGAGAA+AGG + 32965:30091-30110 None:intergenic 45.0%
GAACCGGAACTTGAAGTGAT+AGG + 32965:29737-29756 None:intergenic 45.0%
TCCGGTTTACGTTTCTCATG+AGG + 32965:29776-29795 None:intergenic 45.0%
! AGTGATAGGTTTAGGGCTTG+AGG + 32965:29723-29742 None:intergenic 45.0%
! CGTTTCTCATGAGGTTGGTT+TGG + 32965:29767-29786 None:intergenic 45.0%
! GTGATAGGTTTAGGGCTTGA+GGG + 32965:29722-29741 None:intergenic 45.0%
!! GAGAATGGATTGTCGAGAAG+AGG + 32965:29638-29657 None:intergenic 45.0%
AATCCATTCTCCGCCTTCCA+CGG - 32965:29647-29666 MS.gene60182:CDS 50.0%
ACTCAGACGAACCGAACCAA+GGG - 32965:29795-29814 MS.gene60182:CDS 50.0%
TGGATTGTCGAGAAGAGGGT+CGG + 32965:29633-29652 None:intergenic 50.0%
AGGTCGTGAAAATCCCACGG+CGG + 32965:29680-29699 None:intergenic 55.0%
ATTGTCGAGAAGAGGGTCGG+AGG + 32965:29630-29649 None:intergenic 55.0%
CCGACGCATGATATCGAACC+GGG - 32965:29838-29857 MS.gene60182:CDS 55.0%
CTCAGACGAACCGAACCAAG+GGG - 32965:29796-29815 MS.gene60182:CDS 55.0%
CTTACGCATCCGTGACCTTC+GGG + 32965:29870-29889 None:intergenic 55.0%
GACTCAGACGAACCGAACCA+AGG - 32965:29794-29813 MS.gene60182:CDS 55.0%
GCCGACGCATGATATCGAAC+CGG - 32965:29837-29856 MS.gene60182:CDS 55.0%
GCTTACGCATCCGTGACCTT+CGG + 32965:29871-29890 None:intergenic 55.0%
TTGTCGAGAAGAGGGTCGGA+GGG + 32965:29629-29648 None:intergenic 55.0%
! CCCGGTTCGATATCATGCGT+CGG + 32965:29841-29860 None:intergenic 55.0%
!! TGGTTCGGTTCGTCTGAGTC+CGG + 32965:29794-29813 None:intergenic 55.0%
AGACGAACCGAACCAAGGGG+CGG - 32965:29799-29818 MS.gene60182:CDS 60.0%
GGGAATCAGCCCGAAGGTCA+CGG - 32965:29858-29877 MS.gene60182:CDS 60.0%
GGTCGTGAAAATCCCACGGC+GGG + 32965:29679-29698 None:intergenic 60.0%
TCATCGACCGCCCCTTGGTT+CGG + 32965:29809-29828 None:intergenic 60.0%
! GTGACCTTCGGGCTGATTCC+CGG + 32965:29859-29878 None:intergenic 60.0%
AAGAGGGTCGGAGGGAGGGA+GGG + 32965:29621-29640 None:intergenic 65.0%
CCACCGTGGAAGGCGGAGAA+TGG + 32965:29653-29672 None:intergenic 65.0%
CCATTCTCCGCCTTCCACGG+TGG - 32965:29650-29669 MS.gene60182:CDS 65.0%
CGAACCGGGAATCAGCCCGA+AGG - 32965:29852-29871 MS.gene60182:CDS 65.0%
CGAGAAGAGGGTCGGAGGGA+GGG + 32965:29625-29644 None:intergenic 65.0%
CGGGAAACCACCGTGGAAGG+CGG + 32965:29660-29679 None:intergenic 65.0%
TCGAGAAGAGGGTCGGAGGG+AGG + 32965:29626-29645 None:intergenic 65.0%
TTCGCTCATCGACCGCCCCT+TGG + 32965:29814-29833 None:intergenic 65.0%
CCACGGTGGTTTCCCGCCGT+GGG - 32965:29664-29683 MS.gene60182:CDS 70.0%
CGGCGGGAAACCACCGTGGA+AGG + 32965:29663-29682 None:intergenic 70.0%
GAAGAGGGTCGGAGGGAGGG+AGG + 32965:29622-29641 None:intergenic 70.0%
TCCACGGTGGTTTCCCGCCG+TGG - 32965:29663-29682 MS.gene60182:CDS 70.0%
AGGGTCGGAGGGAGGGAGGG+TGG + 32965:29618-29637 None:intergenic 75.0%
CCCACGGCGGGAAACCACCG+TGG + 32965:29667-29686 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
32965 gene 29608 30132 29608 ID=MS.gene60182
32965 mRNA 29608 30132 29608 ID=MS.gene60182.t1;Parent=MS.gene60182
32965 exon 29608 30132 29608 ID=MS.gene60182.t1.exon1;Parent=MS.gene60182.t1
32965 CDS 29608 30132 29608 ID=cds.MS.gene60182.t1;Parent=MS.gene60182.t1
Gene Sequence

>MS.gene60182

ATGCTCTCCACCCTCCCTCCCTCCGACCCTCTTCTCGACAATCCATTCTCCGCCTTCCACGGTGGTTTCCCGCCGTGGGATTTTCACGACCTATTTTCAGATGATATCAAACCCTCAAGCCCTAAACCTATCACTTCAAGTTCCGGTTCAGATGAACCAAACCAACCTCATGAGAAACGTAAACCGGACTCAGACGAACCGAACCAAGGGGCGGTCGATGAGCGAAAGCGCCGACGCATGATATCGAACCGGGAATCAGCCCGAAGGTCACGGATGCGTAAGCAAAGACATGTTGAGAATCTAAGGAACCAGTTGAACAAGTGTAGAATGGAAAACCGGGAAATGAAAAACCGGTTACAATTCATTTTGTTTCACTTGAACCGGATACGAACCGAAAATGAATGGCTTCGGTCTGAACGAACCATGCTGAACCAAAGAATCAACAACTTTACTCAAATTTTGGTTTGCCAACAATTTCAACCTTTCTCCACTTCATGGACATGTAACACTACTATGGTGGAATAA

Protein sequence

>MS.gene60182.t1

MLSTLPPSDPLLDNPFSAFHGGFPPWDFHDLFSDDIKPSSPKPITSSSGSDEPNQPHEKRKPDSDEPNQGAVDERKRRRMISNRESARRSRMRKQRHVENLRNQLNKCRMENREMKNRLQFILFHLNRIRTENEWLRSERTMLNQRINNFTQILVCQQFQPFSTSWTCNTTMVE