Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60306.t1 | XP_013457865.1 | 97.9 | 233 | 5 | 0 | 1 | 233 | 67 | 299 | 9.80E-104 | 386.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60306.t1 | Q9STS6 | 25.9 | 239 | 131 | 5 | 1 | 196 | 93 | 328 | 5.6e-07 | 56.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60306.t1 | A0A072URD2 | 97.9 | 233 | 5 | 0 | 1 | 233 | 67 | 299 | 7.0e-104 | 386.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene60306.t1 | TF | LOB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60306.t1 | MTR_4g107450 | 99.142 | 233 | 2 | 0 | 1 | 233 | 67 | 299 | 1.87e-173 | 479 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60306.t1 | AT3G47870 | 30.417 | 240 | 119 | 8 | 1 | 196 | 93 | 328 | 9.76e-17 | 78.2 |
Find 41 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATCATTCAAGCTAATTAT+AGG | 0.238366 | 8.2:+18579581 | MS.gene60306:CDS |
| GTGACTTCACAGTTAGAATT+AGG | 0.247178 | 8.2:+18579751 | MS.gene60306:CDS |
| ATCAACAACTTGAAATTTGC+AGG | 0.281961 | 8.2:+18579692 | MS.gene60306:CDS |
| GCAGGCTACAATATCAAATA+TGG | 0.297786 | 8.2:+18579641 | MS.gene60306:CDS |
| AGCAATGCAACCTCATAAAT+TGG | 0.298388 | 8.2:+18580011 | MS.gene60306:CDS |
| GCAATCATACATTTATTCTA+AGG | 0.300515 | 8.2:+18580131 | MS.gene60306:CDS |
| TATCATTCAAGCTAATTATA+GGG | 0.334002 | 8.2:+18579582 | MS.gene60306:CDS |
| GGGCACTGAAGCCACAAATT+AGG | 0.358880 | 8.2:-18579961 | None:intergenic |
| TATCTTCAACAACTCCTTGT+TGG | 0.395214 | 8.2:-18580065 | None:intergenic |
| CTGCTATTGTTATTTGTGTA+AGG | 0.395645 | 8.2:-18579982 | None:intergenic |
| TTGTTATAAGTCTGTGGTTG+TGG | 0.430309 | 8.2:-18579820 | None:intergenic |
| CATCATGAGGTAACTGTTGT+TGG | 0.444844 | 8.2:-18579726 | None:intergenic |
| ATGCTACAAATCCTAATTTG+TGG | 0.446980 | 8.2:+18579950 | MS.gene60306:CDS |
| AAAGAGTGCGAGCGCATTGA+AGG | 0.447294 | 8.2:-18579788 | None:intergenic |
| GATGTTGTGAGGAAATTTGC+AGG | 0.467829 | 8.2:+18579623 | MS.gene60306:CDS |
| TCTGAGGTAACCAATTTATG+AGG | 0.490694 | 8.2:-18580021 | None:intergenic |
| ACAATATCAAATATGGATGA+TGG | 0.492612 | 8.2:+18579648 | MS.gene60306:CDS |
| CAATAACTCCTCCCTCTACA+TGG | 0.493365 | 8.2:+18579915 | MS.gene60306:CDS |
| TGCTATTGTTATTTGTGTAA+GGG | 0.508900 | 8.2:-18579981 | None:intergenic |
| TTGAATGATAATAGAATCCA+TGG | 0.528060 | 8.2:-18579569 | None:intergenic |
| CTCCCTCTACATGGATTCAA+AGG | 0.529912 | 8.2:+18579924 | MS.gene60306:CDS |
| GTAATCCACACTATTGCTGT+TGG | 0.537932 | 8.2:-18579893 | None:intergenic |
| ATCATACATTTATTCTAAGG+AGG | 0.554320 | 8.2:+18580134 | MS.gene60306:CDS |
| CTGATCAGTTGACACTGGTA+AGG | 0.557706 | 8.2:-18580540 | None:intergenic |
| TTATTGTTGTTATAAGTCTG+TGG | 0.569885 | 8.2:-18579826 | None:intergenic |
| TCCTCACAACATCCATGCAC+AGG | 0.573785 | 8.2:-18579613 | None:intergenic |
| CACACTATTGCTGTTGGAGT+AGG | 0.575688 | 8.2:-18579887 | None:intergenic |
| CCTTGTTGGACAGATGATAG+TGG | 0.578651 | 8.2:-18580051 | None:intergenic |
| AGGGATAAGTATCCTGTGCA+TGG | 0.583412 | 8.2:+18579601 | MS.gene60306:CDS |
| TTCACAGTTAGAATTAGGAA+TGG | 0.586596 | 8.2:+18579756 | MS.gene60306:CDS |
| ATCCTTTGAATCCATGTAGA+GGG | 0.590065 | 8.2:-18579926 | None:intergenic |
| CTACTCCAACAGCAATAGTG+TGG | 0.620940 | 8.2:+18579888 | MS.gene60306:CDS |
| CATCCTTTGAATCCATGTAG+AGG | 0.632560 | 8.2:-18579927 | None:intergenic |
| CCACTATCATCTGTCCAACA+AGG | 0.637452 | 8.2:+18580051 | MS.gene60306:CDS |
| TGATAGTGGCTGCGACTCTG+AGG | 0.643276 | 8.2:-18580037 | None:intergenic |
| CTAAGGAGGCTTATGAATCG+AGG | 0.657710 | 8.2:+18580148 | MS.gene60306:CDS |
| TAGTACTGATCAGTTGACAC+TGG | 0.662425 | 8.2:-18580545 | None:intergenic |
| CTTTGAATCCATGTAGAGGG+AGG | 0.666923 | 8.2:-18579923 | None:intergenic |
| TGTGAAGTCACATCATCATG+AGG | 0.679884 | 8.2:-18579739 | None:intergenic |
| TCCTGTGCATGGATGTTGTG+AGG | 0.692042 | 8.2:+18579612 | MS.gene60306:CDS |
| TGTTGCTGAGATACAGACAA+AGG | 0.703585 | 8.2:-18579862 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTGAAATTAGATATAG+TGG | + | chr8.2:18580347-18580366 | MS.gene60306:intron | 20.0% |
| !! | ATGTTGAAATTAGATATAGT+GGG | + | chr8.2:18580348-18580367 | MS.gene60306:intron | 20.0% |
| !! | TATCATTCAAGCTAATTATA+GGG | + | chr8.2:18579582-18579601 | MS.gene60306:CDS | 20.0% |
| !! | TTATCATTCAAGCTAATTAT+AGG | + | chr8.2:18579581-18579600 | MS.gene60306:CDS | 20.0% |
| ! | ACAATATCAAATATGGATGA+TGG | + | chr8.2:18579648-18579667 | MS.gene60306:CDS | 25.0% |
| ! | GCAATCATACATTTATTCTA+AGG | + | chr8.2:18580131-18580150 | MS.gene60306:CDS | 25.0% |
| !! | ATCATACATTTATTCTAAGG+AGG | + | chr8.2:18580134-18580153 | MS.gene60306:CDS | 25.0% |
| !! | TTATTGTTGTTATAAGTCTG+TGG | - | chr8.2:18579829-18579848 | None:intergenic | 25.0% |
| !!! | GTGTTTTTGATCTACTTTAA+CGG | - | chr8.2:18580212-18580231 | None:intergenic | 25.0% |
| !!! | TGCTATTGTTATTTGTGTAA+GGG | - | chr8.2:18579984-18580003 | None:intergenic | 25.0% |
| !!! | TTTTTGATACAGTTGATGAT+AGG | + | chr8.2:18580109-18580128 | MS.gene60306:CDS | 25.0% |
| ATCAACAACTTGAAATTTGC+AGG | + | chr8.2:18579692-18579711 | MS.gene60306:CDS | 30.0% | |
| ATGCTACAAATCCTAATTTG+TGG | + | chr8.2:18579950-18579969 | MS.gene60306:CDS | 30.0% | |
| TTCACAGTTAGAATTAGGAA+TGG | + | chr8.2:18579756-18579775 | MS.gene60306:CDS | 30.0% | |
| ! | TATCAGCATTTTCACTACAA+AGG | - | chr8.2:18580408-18580427 | None:intergenic | 30.0% |
| !! | CTGCTATTGTTATTTGTGTA+AGG | - | chr8.2:18579985-18580004 | None:intergenic | 30.0% |
| AGCAATGCAACCTCATAAAT+TGG | + | chr8.2:18580011-18580030 | MS.gene60306:CDS | 35.0% | |
| ATCCTTTGAATCCATGTAGA+GGG | - | chr8.2:18579929-18579948 | None:intergenic | 35.0% | |
| GCAGGCTACAATATCAAATA+TGG | + | chr8.2:18579641-18579660 | MS.gene60306:CDS | 35.0% | |
| GCTTATGAATCGAGGTAAAT+TGG | + | chr8.2:18580156-18580175 | MS.gene60306:intron | 35.0% | |
| GTGACTTCACAGTTAGAATT+AGG | + | chr8.2:18579751-18579770 | MS.gene60306:CDS | 35.0% | |
| TATCTTCAACAACTCCTTGT+TGG | - | chr8.2:18580068-18580087 | None:intergenic | 35.0% | |
| TTGTTATAAGTCTGTGGTTG+TGG | - | chr8.2:18579823-18579842 | None:intergenic | 35.0% | |
| ! | TCTGAGGTAACCAATTTATG+AGG | - | chr8.2:18580024-18580043 | None:intergenic | 35.0% |
| CATCATGAGGTAACTGTTGT+TGG | - | chr8.2:18579729-18579748 | None:intergenic | 40.0% | |
| CATCCTTTGAATCCATGTAG+AGG | - | chr8.2:18579930-18579949 | None:intergenic | 40.0% | |
| CTAGATGATCATTGATCCAC+AGG | + | chr8.2:18580239-18580258 | MS.gene60306:intron | 40.0% | |
| GATGTTGTGAGGAAATTTGC+AGG | + | chr8.2:18579623-18579642 | MS.gene60306:CDS | 40.0% | |
| GTAATCCACACTATTGCTGT+TGG | - | chr8.2:18579896-18579915 | None:intergenic | 40.0% | |
| TCTCATTTAGTACGACCACT+CGG | - | chr8.2:18580317-18580336 | None:intergenic | 40.0% | |
| TGTGAAGTCACATCATCATG+AGG | - | chr8.2:18579742-18579761 | None:intergenic | 40.0% | |
| TGTTGCTGAGATACAGACAA+AGG | - | chr8.2:18579865-18579884 | None:intergenic | 40.0% | |
| ACAAGATTACCTCTGGTCGA+GGG | - | chr8.2:18580267-18580286 | None:intergenic | 45.0% | |
| ACCAGAGGTAATCTTGTCAG+GGG | + | chr8.2:18580270-18580289 | MS.gene60306:intron | 45.0% | |
| AGGGATAAGTATCCTGTGCA+TGG | + | chr8.2:18579601-18579620 | MS.gene60306:CDS | 45.0% | |
| CAATAACTCCTCCCTCTACA+TGG | + | chr8.2:18579915-18579934 | MS.gene60306:CDS | 45.0% | |
| CCACTATCATCTGTCCAACA+AGG | + | chr8.2:18580051-18580070 | MS.gene60306:CDS | 45.0% | |
| CCTTGTTGGACAGATGATAG+TGG | - | chr8.2:18580054-18580073 | None:intergenic | 45.0% | |
| CTAAGGAGGCTTATGAATCG+AGG | + | chr8.2:18580148-18580167 | MS.gene60306:CDS | 45.0% | |
| CTACTCCAACAGCAATAGTG+TGG | + | chr8.2:18579888-18579907 | MS.gene60306:CDS | 45.0% | |
| CTCATTTAGTACGACCACTC+GGG | - | chr8.2:18580316-18580335 | None:intergenic | 45.0% | |
| CTCCCTCTACATGGATTCAA+AGG | + | chr8.2:18579924-18579943 | MS.gene60306:CDS | 45.0% | |
| CTTTGAATCCATGTAGAGGG+AGG | - | chr8.2:18579926-18579945 | None:intergenic | 45.0% | |
| GACCAGAGGTAATCTTGTCA+GGG | + | chr8.2:18580269-18580288 | MS.gene60306:intron | 45.0% | |
| GTACATTTCTGCCTGCAGTT+CGG | + | chr8.2:18580438-18580457 | MS.gene60306:intron | 45.0% | |
| TCATTTAGTACGACCACTCG+GGG | - | chr8.2:18580315-18580334 | None:intergenic | 45.0% | |
| ! | CACACTATTGCTGTTGGAGT+AGG | - | chr8.2:18579890-18579909 | None:intergenic | 45.0% |
| ACCCCTGACAAGATTACCTC+TGG | - | chr8.2:18580274-18580293 | None:intergenic | 50.0% | |
| CGACCAGAGGTAATCTTGTC+AGG | + | chr8.2:18580268-18580287 | MS.gene60306:intron | 50.0% | |
| GACAAGATTACCTCTGGTCG+AGG | - | chr8.2:18580268-18580287 | None:intergenic | 50.0% | |
| TCCTCACAACATCCATGCAC+AGG | - | chr8.2:18579616-18579635 | None:intergenic | 50.0% | |
| TCCTGTGCATGGATGTTGTG+AGG | + | chr8.2:18579612-18579631 | MS.gene60306:CDS | 50.0% | |
| ! | AAAGAGTGCGAGCGCATTGA+AGG | - | chr8.2:18579791-18579810 | None:intergenic | 50.0% |
| !! | GGGCACTGAAGCCACAAATT+AGG | - | chr8.2:18579964-18579983 | None:intergenic | 50.0% |
| CAGCGATTCTTCCGAACTGC+AGG | - | chr8.2:18580452-18580471 | None:intergenic | 55.0% | |
| CCAATTCTAACCTCCCCGAG+TGG | + | chr8.2:18580299-18580318 | MS.gene60306:intron | 55.0% | |
| CCACTCGGGGAGGTTAGAAT+TGG | - | chr8.2:18580302-18580321 | None:intergenic | 55.0% | |
| TTTAGTACGACCACTCGGGG+AGG | - | chr8.2:18580312-18580331 | None:intergenic | 55.0% | |
| !! | TGATAGTGGCTGCGACTCTG+AGG | - | chr8.2:18580040-18580059 | None:intergenic | 55.0% |
| CCACAGGTTCCCTCGACCAG+AGG | + | chr8.2:18580255-18580274 | MS.gene60306:intron | 65.0% | |
| CCTCTGGTCGAGGGAACCTG+TGG | - | chr8.2:18580258-18580277 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 18579571 | 18580558 | 18579571 | ID=MS.gene60306 |
| chr8.2 | mRNA | 18579571 | 18580558 | 18579571 | ID=MS.gene60306.t1;Parent=MS.gene60306 |
| chr8.2 | exon | 18579571 | 18580169 | 18579571 | ID=MS.gene60306.t1.exon1;Parent=MS.gene60306.t1 |
| chr8.2 | CDS | 18579571 | 18580169 | 18579571 | ID=cds.MS.gene60306.t1;Parent=MS.gene60306.t1 |
| chr8.2 | exon | 18580456 | 18580558 | 18580456 | ID=MS.gene60306.t1.exon2;Parent=MS.gene60306.t1 |
| chr8.2 | CDS | 18580456 | 18580558 | 18580456 | ID=cds.MS.gene60306.t1;Parent=MS.gene60306.t1 |
| Gene Sequence |
| Protein sequence |
