AlfalfaGEDB | MS.gene60412

M. sativa cultivar XinJiangDaYe / MS.gene60412

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60412.t1	XP_013442259.1	92.9	380	27	0	1	380	1	380	3.60E-164	587.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60412.t1	Q1PF17	43.0	328	146	7	55	375	10	303	3.0e-26	120.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60412.t1	A0A072TFG1	92.9	380	27	0	1	380	1	380	2.6e-164	587.8

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene60412.t1	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60412.t1	MTR_0246s0020	92.895	380	27	0	1	380	1	380	0.0	660
MS.gene60412.t1	MTR_0246s0020	89.757	371	36	1	1	371	1	369	0.0	612
MS.gene60412.t1	MTR_6g047570	57.474	388	104	8	1	379	1	336	3.75e-135	389
MS.gene60412.t1	MTR_0011s0260	58.763	388	121	10	1	378	1	359	4.62e-127	370
MS.gene60412.t1	MTR_0011s0210	58.462	390	123	10	1	380	1	361	3.92e-126	367
MS.gene60412.t1	MTR_4g067010	53.109	386	105	10	1	377	1	319	3.60e-115	338
MS.gene60412.t1	MTR_4g098035	50.886	395	125	14	1	379	1	342	9.01e-109	323
MS.gene60412.t1	MTR_4g066460	53.351	388	113	10	1	379	1	329	2.34e-107	318
MS.gene60412.t1	MTR_4g066380	51.026	390	120	9	1	381	1	328	1.23e-104	311
MS.gene60412.t1	MTR_0246s0050	49.731	372	120	12	23	379	2	321	1.41e-99	298

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60412.t1	AT4G37850	38.276	290	143	10	101	377	62	328	4.86e-50	171
MS.gene60412.t1	AT4G37850	38.276	290	143	10	101	377	62	328	4.86e-50	171
MS.gene60412.t1	AT2G22750	36.508	378	184	16	7	376	3	332	3.36e-43	153
MS.gene60412.t1	AT2G22750	45.128	195	106	1	183	376	110	304	3.64e-43	152
MS.gene60412.t1	AT2G22750	45.408	196	104	3	183	376	134	328	5.74e-43	152
MS.gene60412.t1	AT2G22750	45.128	195	105	2	183	376	110	303	8.74e-42	149
MS.gene60412.t1	AT2G22750	36.508	378	183	17	7	376	3	331	1.26e-41	149
MS.gene60412.t1	AT2G22760	41.969	193	104	5	186	374	107	295	5.16e-40	144
MS.gene60412.t1	AT2G22760	42.442	172	92	5	186	354	107	274	1.57e-33	126
MS.gene60412.t1	AT2G22770	32.602	319	142	11	82	372	44	317	1.14e-29	117

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Find 65 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTCCTTGATGCTAATG+AGG	0.524526	8.2:+24129816	MS.gene60412:CDS
AAAGATGAGTTAGATGATGA+TGG	0.577216	8.2:-24130039	None:intergenic
AAGTGCAAGCAAAAGTGTCA+GGG	0.633121	8.2:+24130878	MS.gene60412:CDS
AAGTTTAGGTTGCATTCTTC+AGG	0.308908	8.2:-24129799	None:intergenic
AATAGCAGTGTCTTACCCTT+TGG	0.385303	8.2:+24130994	MS.gene60412:CDS
AATGTCAATGGAATCCCCAA+AGG	0.614554	8.2:-24131010	None:intergenic
ACATGAATGTTTCTCATTTG+GGG	0.622328	8.2:-24130189	None:intergenic
ACTCTTCCTCATTAGCATCA+AGG	0.553454	8.2:-24129822	None:intergenic
ACTTCAATGCTGCCATGCGT+AGG	0.601033	8.2:-24132038	None:intergenic
ACTTGTCAAGAACCTACGCA+TGG	0.686710	8.2:+24132026	MS.gene60412:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGATGAGTTAGATGATGA+TGG	-	chr8.2:24130042-24130061	None:intergenic	30.0%
AATATCATTAATCAATGCCC+CGG	+	chr8.2:24131324-24131343	MS.gene60412:intron	30.0%
AATTAATGAAATGCTAACCG+GGG	-	chr8.2:24131344-24131363	None:intergenic	30.0%
ACATGAATGTTTCTCATTTG+GGG	-	chr8.2:24130192-24130211	None:intergenic	30.0%
AGACATGAATGTTTCTCATT+TGG	-	chr8.2:24130194-24130213	None:intergenic	30.0%
AGCAATGATAGTAATGTCAA+TGG	-	chr8.2:24131025-24131044	None:intergenic	30.0%
ATTATTAGGGTTATGCTAAC+CGG	+	chr8.2:24131177-24131196	MS.gene60412:intron	30.0%
CAAAAACATCTCAAACCAAA+AGG	+	chr8.2:24130259-24130278	MS.gene60412:CDS	30.0%
CATCATCATCAATGATACTA+TGG	-	chr8.2:24130798-24130817	None:intergenic	30.0%
GACATGAATGTTTCTCATTT+GGG	-	chr8.2:24130193-24130212	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	24129617	24132083	24129617	ID=MS.gene60412
chr8.2	mRNA	24129617	24132083	24129617	ID=MS.gene60412.t1;Parent=MS.gene60412
chr8.2	exon	24129617	24129682	24129617	ID=MS.gene60412.t1.exon1;Parent=MS.gene60412.t1
chr8.2	CDS	24129617	24129682	24129617	ID=cds.MS.gene60412.t1;Parent=MS.gene60412.t1
chr8.2	exon	24129775	24130386	24129775	ID=MS.gene60412.t1.exon2;Parent=MS.gene60412.t1
chr8.2	CDS	24129775	24130386	24129775	ID=cds.MS.gene60412.t1;Parent=MS.gene60412.t1
chr8.2	exon	24130667	24131047	24130667	ID=MS.gene60412.t1.exon3;Parent=MS.gene60412.t1
chr8.2	CDS	24130667	24131047	24130667	ID=cds.MS.gene60412.t1;Parent=MS.gene60412.t1
chr8.2	exon	24131994	24132083	24131994	ID=MS.gene60412.t1.exon4;Parent=MS.gene60412.t1
chr8.2	CDS	24131994	24132083	24131994	ID=cds.MS.gene60412.t1;Parent=MS.gene60412.t1

Gene Sequence

>MS.gene60412

ATGGAAGAAATCAACAACACACCAATGAACGTATCAGAAGAAACCAGCAAATGGCTTTCTGATTTGGTAAGAAAATTAAAGGCAAATTAACATATTATTGATTATATAAGTAAATTCTTTATCATTTTAAATTTTAATTTTTTTTTTTGTTGTTGCAGGAAATGGATGAGTATAATTTGTTTCCTGAAGAATGCAACCTAAACTTCCTTGATGCTAATGAGGAAGAGTTTCTTCCACAAGAACAAACCCAACAACAATGTTTGAGTTCAGAGTCCAATTCCACAACTATCACCAACTCATTCACTGATGAAACAACTTTTGACTCTTTTGACTTTGGTTTTGAAATTGAGAGACCAACCATGGAGCTGAAAACAATTGTTAGTGACAACAACATCATTGAAACCATTTCACCAAAACTTTCTCCATCATCATCTAACTCATCTTTGCACTCTCAGATTTTGTCTTTTGACAACCTCCCAAATTCTCCTCCTACCAACACCCCCCAGTTTTGTGGACTCACTCCTGCCTTAAACTCAAAGTCAAAACAGAACAAAACAGTGTCAGTGTTTCCACCCCAAATGAGAAACATTCATGTCTCAACTCAAAATCCTAAAGGGTTATCCAAAACTCAAAACTTTGCAACAAAAACATCTCAAACCAAAAGGTCTCGAGCCAACGCCGATGACCATATCATGGCTGAAAGAAAGCGAAGAGAGAAACTTAGCCAAAGCTTCATTGCTCTTGCAGCTCTTGTTCCTAACCTGAAAAAGGTAACACTTCACACTTATGTTTGAAGGCGTGTCTCATGTTTGTCACGTATCAGTGTCCAACACGACACTAATACACATTATTACATACAATAATGTCAATTTTACGAATAATTAGTAATATTGACATGTTACTGTCGATGTTGTGTCTTGGTGTCTGTGTTAAGACAATAAATCAATTTTGATTACCCCACTAGCTAAATAGCACTCAAATCATTGTATTTTTATTTTCTTCATGAATTGTTAACATCTTCTGATCAAATACTGTTATAAAATTTGGTAGATGGACAAGGCTTCTGTATTAGCTGAGTCTATCACATACGTGAAAGAGCTTAAAAAGCGTTTGGAAGTTTTGGAAGAACAAAACAAGAAAACAAAGGTAGAGTCCGTGGTTGTTCTGAAGAAACCAGACCATAGTATCATTGATGATGATGATGATGATGATAGCTCATCATATGATGAGAGTATTGAAGGTGCTACTGATTCATCCTTTCAAGTGCAAGCAAAAGTGTCAGGGAAAGAGATGCTGATTCGGATTCACTGCCAGAAGCACAAGGGGATTCTGGTGAAAGTCATGGCTGAGATTCAAAGCATACAATTGTTTGCTGTGAATAGCAGTGTCTTACCCTTTGGGGATTCCATTGACATTACTATCATTGCTCAGGTAAAGGACAATCTCTACCTACCTTAATAATTGCCACTAAATATTCATTGTTTAAAAGAGAATGCCACTAAACATTTTTTCACTACTTTTTAAGTATTATTATTTTGTTTGAGGCACTAATTATTATTAATTATTAGGGTTATGCTAACCGGTACTCTGAGAAACTTGTTAAGAATAAAAAAAGGAATTTATTACCATAAAAGGAAACTTCGTTAACTTATTTATGAATTAATTACACAGTTTTCAATGCAATAATTACTATATAATTTTCTTTTTAATATCATTAATCAATGCCCCGGTTAGCATTTCATTAATTATTATTATCATTATTACATTTTTTGTCTAGGGGCCAAACGTGTCACTCAGATGACCCTCGCTTTCAAAATCAATGTTGTTTTAGTCTAAAAAATTAGAATAAGTGTTGTTTTATTTTTCTATTGTAGTGTTAGTCAATTTATTTTAATTTTACTATTAAACTTGTGTTGTTTTTTTTTTGGAAAATGTTAACTTGTGCCCTTAAGGCACAAGTTAATTATTGTAGTATAGAAATAATTTATTGGAGTGTGTGCAATGTATTTAATTTTTAACTTTTTAGTCAATTAAATGCACAAAATTTTAAGGAATACTTCTTCTTTTGGGGTTTTTTAACATGTGCCCTAAGGGCACAAGTTAACACTACTTTTTTTTTATATCAGGATGTTAAATTTATGTTGCAATACATCATCACTTCCAATTAATTATAACAGCCAACAATTATTGACGATACTTTTAAAAAATTATTACACGTATTCATGAAAGGGAGGGCGTATTTTATTCTAGGCTGTTTTCTATCTGTTTCTTTTTAGGAATTTTGTTTAGGAGTATCTTTTTTTTTTATTATTATTTTTGTCTTATTTGCCCACGAGTTGTTTAATTGTATTAAAATACTATTGTTATACTTTTGTAGATGGGTGAAGGGTACAACTTGAGCATAAAGGAACTTGTCAAGAACCTACGCATGGCAGCATTGAAGTTTATGTCATCAAAGCATGAATGA

Protein sequence

>MS.gene60412.t1

MEEINNTPMNVSEETSKWLSDLEMDEYNLFPEECNLNFLDANEEEFLPQEQTQQQCLSSESNSTTITNSFTDETTFDSFDFGFEIERPTMELKTIVSDNNIIETISPKLSPSSSNSSLHSQILSFDNLPNSPPTNTPQFCGLTPALNSKSKQNKTVSVFPPQMRNIHVSTQNPKGLSKTQNFATKTSQTKRSRANADDHIMAERKRREKLSQSFIALAALVPNLKKMDKASVLAESITYVKELKKRLEVLEEQNKKTKVESVVVLKKPDHSIIDDDDDDDSSSYDESIEGATDSSFQVQAKVSGKEMLIRIHCQKHKGILVKVMAEIQSIQLFAVNSSVLPFGDSIDITIIAQMGEGYNLSIKELVKNLRMAALKFMSSKHE