AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60412

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60412.t1 MTR_0246s0020 92.895 380 27 0 1 380 1 380 0.0 660
MS.gene60412.t1 MTR_0246s0020 89.757 371 36 1 1 371 1 369 0.0 612
MS.gene60412.t1 MTR_6g047570 57.474 388 104 8 1 379 1 336 3.75e-135 389
MS.gene60412.t1 MTR_0011s0260 58.763 388 121 10 1 378 1 359 4.62e-127 370
MS.gene60412.t1 MTR_0011s0210 58.462 390 123 10 1 380 1 361 3.92e-126 367
MS.gene60412.t1 MTR_4g067010 53.109 386 105 10 1 377 1 319 3.60e-115 338
MS.gene60412.t1 MTR_4g098035 50.886 395 125 14 1 379 1 342 9.01e-109 323
MS.gene60412.t1 MTR_4g066460 53.351 388 113 10 1 379 1 329 2.34e-107 318
MS.gene60412.t1 MTR_4g066380 51.026 390 120 9 1 381 1 328 1.23e-104 311
MS.gene60412.t1 MTR_0246s0050 49.731 372 120 12 23 379 2 321 1.41e-99 298
MS.gene60412.t1 MTR_0250s0040 48.883 358 88 9 24 379 1 265 6.93e-91 274
MS.gene60412.t1 MTR_6g047550 57.091 275 73 7 1 265 1 240 3.38e-88 268
MS.gene60412.t1 MTR_5g014520 51.351 259 99 6 121 378 101 333 5.98e-67 215
MS.gene60412.t1 MTR_5g014560 49.383 243 93 8 147 378 84 307 8.78e-60 196
MS.gene60412.t1 MTR_4g009540 50.000 188 90 3 191 375 145 331 3.93e-54 182
MS.gene60412.t1 MTR_2g010450 49.462 186 83 4 196 377 175 353 7.99e-53 179
MS.gene60412.t1 MTR_5g014600 36.340 377 177 14 13 374 6 334 1.06e-52 178
MS.gene60412.t1 MTR_4g009110 39.700 267 125 5 116 375 113 350 3.39e-48 167
MS.gene60412.t1 MTR_2g104490 40.304 263 129 7 108 369 67 302 3.58e-48 166
MS.gene60412.t1 MTR_4g009540 50.299 167 79 3 191 354 145 310 6.55e-46 160
MS.gene60412.t1 MTR_8g009020 38.603 272 125 8 111 370 77 318 1.09e-44 157
MS.gene60412.t1 MTR_4g097940 47.340 188 87 6 185 369 142 320 5.55e-44 155
MS.gene60412.t1 MTR_2g104550 38.462 273 141 7 108 378 68 315 1.04e-43 154
MS.gene60412.t1 MTR_4g092700 44.949 198 101 4 182 377 142 333 1.30e-43 154
MS.gene60412.t1 MTR_4g097920 45.745 188 90 6 185 369 127 305 1.33e-43 154
MS.gene60412.t1 MTR_4g097920 45.745 188 90 6 185 369 142 320 1.55e-43 154
MS.gene60412.t1 MTR_4g097950 41.729 266 120 10 120 376 12 251 8.03e-43 150
MS.gene60412.t1 MTR_2g104490 39.113 248 123 7 108 354 67 287 1.50e-40 145
MS.gene60412.t1 MTR_5g014640 34.139 331 160 13 68 374 27 323 4.53e-40 145
MS.gene60412.t1 MTR_8g069740 37.931 203 115 4 153 353 72 265 2.48e-39 141
MS.gene60412.t1 MTR_2g104650 39.171 217 128 2 159 373 53 267 5.79e-39 140
MS.gene60412.t1 MTR_2g104560 38.073 218 109 7 167 376 27 226 7.16e-39 139
MS.gene60412.t1 MTR_7g080780 31.532 333 178 8 74 382 26 332 1.11e-37 139
MS.gene60412.t1 MTR_7g080780 31.532 333 178 8 74 382 13 319 1.28e-37 138
MS.gene60412.t1 MTR_2g104500 33.813 278 125 8 106 375 46 272 1.99e-32 123
MS.gene60412.t1 MTR_8g009010 35.317 252 99 6 122 370 77 267 1.62e-31 122
MS.gene60412.t1 MTR_2g104530 34.975 203 115 3 169 370 83 269 3.20e-31 120
MS.gene60412.t1 MTR_2g104590 33.929 224 110 7 161 373 26 222 5.79e-30 115
MS.gene60412.t1 MTR_4g009510 40.741 162 63 5 221 372 64 202 3.90e-27 107
MS.gene60412.t1 MTR_7g451650 38.509 161 67 4 221 372 57 194 3.04e-25 102
MS.gene60412.t1 MTR_5g014550 47.287 129 58 3 228 353 54 175 4.56e-24 98.6
MS.gene60412.t1 MTR_7g050350 37.419 155 65 4 227 372 1 132 1.80e-23 95.1
MS.gene60412.t1 MTR_7g451650 37.419 155 65 4 227 372 1 132 1.80e-23 95.1
MS.gene60412.t1 MTR_4g009420 36.875 160 63 4 221 372 65 194 2.22e-23 96.7
MS.gene60412.t1 MTR_1g072320 29.612 206 129 5 178 369 445 648 2.95e-18 87.0
MS.gene60412.t1 MTR_8g067280 35.976 164 89 4 190 339 448 609 1.23e-17 85.1
MS.gene60412.t1 MTR_7g451650 35.664 143 60 4 221 354 57 176 2.23e-16 77.0
MS.gene60412.t1 MTR_8g024790 29.730 148 88 4 198 339 291 428 2.84e-16 80.5
MS.gene60412.t1 MTR_5g030430 30.978 184 103 4 190 355 479 656 1.78e-14 75.5
MS.gene60412.t1 MTR_8g027495 48.438 64 33 0 198 261 458 521 7.69e-14 73.6
MS.gene60412.t1 MTR_7g117670 53.571 56 26 0 198 253 402 457 1.12e-13 72.8
MS.gene60412.t1 MTR_7g096350 48.529 68 29 1 190 251 341 408 1.99e-13 72.0
MS.gene60412.t1 MTR_8g064700 25.595 168 112 5 191 351 237 398 1.45e-12 68.9
MS.gene60412.t1 MTR_3g498695 25.397 189 135 2 183 365 283 471 1.64e-12 68.9
MS.gene60412.t1 MTR_1g112680 25.434 173 106 3 190 339 309 481 1.76e-12 68.9
MS.gene60412.t1 MTR_8g024770 33.051 118 70 4 198 311 279 391 2.42e-12 68.6
MS.gene60412.t1 MTR_3g498695 26.452 155 109 1 183 332 283 437 6.62e-12 67.0
MS.gene60412.t1 MTR_3g030700 26.554 177 125 4 180 353 178 352 2.59e-11 65.1
MS.gene60412.t1 MTR_7g110810 46.875 64 34 0 187 250 201 264 3.73e-11 64.3
MS.gene60412.t1 MTR_1g019240 36.538 104 63 2 153 254 49 151 5.02e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60412.t1 AT4G37850 38.276 290 143 10 101 377 62 328 4.86e-50 171
MS.gene60412.t1 AT4G37850 38.276 290 143 10 101 377 62 328 4.86e-50 171
MS.gene60412.t1 AT2G22750 36.508 378 184 16 7 376 3 332 3.36e-43 153
MS.gene60412.t1 AT2G22750 45.128 195 106 1 183 376 110 304 3.64e-43 152
MS.gene60412.t1 AT2G22750 45.408 196 104 3 183 376 134 328 5.74e-43 152
MS.gene60412.t1 AT2G22750 45.128 195 105 2 183 376 110 303 8.74e-42 149
MS.gene60412.t1 AT2G22750 36.508 378 183 17 7 376 3 331 1.26e-41 149
MS.gene60412.t1 AT2G22760 41.969 193 104 5 186 374 107 295 5.16e-40 144
MS.gene60412.t1 AT2G22760 42.442 172 92 5 186 354 107 274 1.57e-33 126
MS.gene60412.t1 AT2G22770 32.602 319 142 11 82 372 44 317 1.14e-29 117
MS.gene60412.t1 AT4G17880 31.429 175 103 3 190 355 402 568 9.84e-16 79.3
MS.gene60412.t1 AT1G32640 33.117 154 83 4 190 332 438 582 1.60e-15 78.6
MS.gene60412.t1 AT5G46760 29.231 195 109 7 190 358 401 592 2.58e-15 77.8
MS.gene60412.t1 AT1G01260 36.066 122 51 3 192 313 427 521 6.34e-15 76.6
MS.gene60412.t1 AT1G01260 36.066 122 51 3 192 313 427 521 6.34e-15 76.6
MS.gene60412.t1 AT1G01260 36.066 122 51 3 192 313 427 521 6.34e-15 76.6
MS.gene60412.t1 AT4G16430 50.746 67 33 0 192 258 314 380 1.33e-14 75.5
MS.gene60412.t1 AT2G46510 45.946 74 34 1 190 257 381 454 2.04e-14 75.1
MS.gene60412.t1 AT1G63650 28.025 157 82 4 198 332 405 552 1.94e-13 72.0
MS.gene60412.t1 AT1G63650 28.025 157 82 4 198 332 405 552 1.94e-13 72.0
MS.gene60412.t1 AT1G63650 28.025 157 82 4 198 332 405 552 1.94e-13 72.0
MS.gene60412.t1 AT4G09820 30.000 160 73 4 189 339 352 481 5.35e-13 70.9
MS.gene60412.t1 AT2G16910 28.049 164 95 4 199 342 315 475 2.74e-12 68.6
MS.gene60412.t1 AT5G41315 24.623 199 121 5 192 367 435 627 3.19e-12 68.6
MS.gene60412.t1 AT5G41315 24.623 199 121 5 192 367 440 632 3.29e-12 68.6
MS.gene60412.t1 AT5G41315 24.623 199 121 5 192 367 443 635 3.57e-12 68.2
MS.gene60412.t1 AT4G00870 32.800 125 64 2 199 314 250 363 5.50e-12 67.4
MS.gene60412.t1 AT3G26744 27.273 165 114 2 199 357 308 472 7.45e-12 67.0
MS.gene60412.t1 AT3G26744 27.273 165 114 2 199 357 308 472 7.45e-12 67.0
MS.gene60412.t1 AT3G26744 27.273 165 114 2 199 357 308 472 7.45e-12 67.0
MS.gene60412.t1 AT3G26744 28.058 139 95 1 199 332 308 446 1.78e-11 65.9
MS.gene60412.t1 AT5G57150 27.811 169 108 4 196 355 53 216 4.60e-11 62.4
MS.gene60412.t1 AT2G28160 28.144 167 102 5 179 340 117 270 4.79e-11 63.5
MS.gene60412.t1 AT2G28160 28.144 167 102 5 179 340 117 270 5.16e-11 63.5
MS.gene60412.t1 AT5G57150 28.235 170 108 4 196 356 52 216 7.79e-11 62.4
MS.gene60412.t1 AT5G57150 27.976 168 107 4 196 354 53 215 8.04e-11 62.0
MS.gene60412.t1 AT5G57150 26.380 163 116 2 196 354 52 214 9.03e-11 61.6
MS.gene60412.t1 AT5G57150 26.380 163 116 2 196 354 53 215 9.43e-11 62.4

Find 65 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGAATCCCCTTGTGCTTC+TGG 0.171911 8.2:-24130927 None:intergenic
GTGTTGGTAGGAGGAGAATT+TGG 0.206656 8.2:-24130093 None:intergenic
CAGCAAATGGCTTTCTGATT+TGG 0.214444 8.2:+24129661 MS.gene60412:CDS
GACATGAATGTTTCTCATTT+GGG 0.281293 8.2:-24130190 None:intergenic
AGACATGAATGTTTCTCATT+TGG 0.284212 8.2:-24130191 None:intergenic
CATTAGCATCAAGGAAGTTT+AGG 0.295193 8.2:-24129813 None:intergenic
AAGTTTAGGTTGCATTCTTC+AGG 0.308908 8.2:-24129799 None:intergenic
CCAGAAGCACAAGGGGATTC+TGG 0.313026 8.2:+24130927 MS.gene60412:CDS
TGTTGGTAGGAGGAGAATTT+GGG 0.316269 8.2:-24130092 None:intergenic
TCTTGTTCCTAACCTGAAAA+AGG 0.316557 8.2:+24130365 MS.gene60412:CDS
GTTTCTTCTGATACGTTCAT+TGG 0.319465 8.2:-24129638 None:intergenic
CAGGAGTGAGTCCACAAAAC+TGG 0.341350 8.2:-24130119 None:intergenic
ATAGCAGTGTCTTACCCTTT+GGG 0.356671 8.2:+24130995 MS.gene60412:CDS
CAAATCAGAAAGCCATTTGC+TGG 0.362893 8.2:-24129660 None:intergenic
GGCGTTGGCTCGAGACCTTT+TGG 0.371700 8.2:-24130274 None:intergenic
GATGATGATGGAGAAAGTTT+TGG 0.378456 8.2:-24130027 None:intergenic
AATAGCAGTGTCTTACCCTT+TGG 0.385303 8.2:+24130994 MS.gene60412:CDS
GCTTTCTTTCAGCCATGATA+TGG 0.385864 8.2:-24130302 None:intergenic
CTGAACTCAAACATTGTTGT+TGG 0.389224 8.2:-24129864 None:intergenic
CTGCAAGAGCAATGAAGCTT+TGG 0.405262 8.2:-24130341 None:intergenic
CATCATCATCAATGATACTA+TGG 0.407350 8.2:-24130795 None:intergenic
GTCTCAACTCAAAATCCTAA+AGG 0.422120 8.2:+24130210 MS.gene60412:CDS
GTTGGTGATAGTTGTGGAAT+TGG 0.425475 8.2:-24129890 None:intergenic
TGAACTCAAACATTGTTGTT+GGG 0.428381 8.2:-24129863 None:intergenic
CAGGGAAAGAGATGCTGATT+CGG 0.434047 8.2:+24130896 MS.gene60412:CDS
CAAGTGCAAGCAAAAGTGTC+AGG 0.441082 8.2:+24130877 MS.gene60412:CDS
AGGAGTGAGTCCACAAAACT+GGG 0.451243 8.2:-24130118 None:intergenic
TCTCAACTCAAAATCCTAAA+GGG 0.458505 8.2:+24130211 MS.gene60412:CDS
TCATATGATGAGAGTATTGA+AGG 0.466240 8.2:+24130835 MS.gene60412:CDS
TGTTGTTGGGTTTGTTCTTG+TGG 0.480560 8.2:-24129850 None:intergenic
TGACATTACTATCATTGCTC+AGG 0.494765 8.2:+24131026 MS.gene60412:CDS
AGCAATGATAGTAATGTCAA+TGG 0.495292 8.2:-24131022 None:intergenic
TTTGACTTTGAGTTTAAGGC+AGG 0.501893 8.2:-24130138 None:intergenic
TTCAGCCATGATATGGTCAT+CGG 0.521311 8.2:-24130295 None:intergenic
CATGATATGGTCATCGGCGT+TGG 0.524246 8.2:-24130289 None:intergenic
AAACTTCCTTGATGCTAATG+AGG 0.524526 8.2:+24129816 MS.gene60412:CDS
TCATCAATGATACTATGGTC+TGG 0.549545 8.2:-24130790 None:intergenic
ACTCTTCCTCATTAGCATCA+AGG 0.553454 8.2:-24129822 None:intergenic
TATCAGAAGAAACCAGCAAA+TGG 0.554188 8.2:+24129648 MS.gene60412:CDS
TGAATGTTTCTCATTTGGGG+TGG 0.558418 8.2:-24130186 None:intergenic
CAACGCCGATGACCATATCA+TGG 0.570050 8.2:+24130290 MS.gene60412:CDS
AAAGATGAGTTAGATGATGA+TGG 0.577216 8.2:-24130039 None:intergenic
TAGCAGTGTCTTACCCTTTG+GGG 0.577291 8.2:+24130996 MS.gene60412:CDS
TAAAATTTGGTAGATGGACA+AGG 0.590351 8.2:+24130654 MS.gene60412:intron
ATGTCAATGGAATCCCCAAA+GGG 0.596532 8.2:-24131009 None:intergenic
AGGGTACAACTTGAGCATAA+AGG 0.600355 8.2:+24132002 MS.gene60412:CDS
ACTTCAATGCTGCCATGCGT+AGG 0.601033 8.2:-24132038 None:intergenic
TGAAATTGAGAGACCAACCA+TGG 0.603140 8.2:+24129957 MS.gene60412:CDS
TGGTAGGAGGAGAATTTGGG+AGG 0.603292 8.2:-24130089 None:intergenic
GAATGAGTTGGTGATAGTTG+TGG 0.603767 8.2:-24129896 None:intergenic
GGGGATTCTGGTGAAAGTCA+TGG 0.606251 8.2:+24130939 MS.gene60412:CDS
GATTCACTGCCAGAAGCACA+AGG 0.606463 8.2:+24130918 MS.gene60412:CDS
TTCACTGCCAGAAGCACAAG+GGG 0.606586 8.2:+24130920 MS.gene60412:CDS
AATGTCAATGGAATCCCCAA+AGG 0.614554 8.2:-24131010 None:intergenic
TGTTTCATCAGTGAATGAGT+TGG 0.619624 8.2:-24129908 None:intergenic
ACATGAATGTTTCTCATTTG+GGG 0.622328 8.2:-24130189 None:intergenic
AAGTGCAAGCAAAAGTGTCA+GGG 0.633121 8.2:+24130878 MS.gene60412:CDS
GAAAACAAAGGTAGAGTCCG+TGG 0.640082 8.2:+24130753 MS.gene60412:CDS
GAGTGAGTCCACAAAACTGG+GGG 0.649560 8.2:-24130116 None:intergenic
TGGTTTCTTCAGAACAACCA+CGG 0.659948 8.2:-24130770 None:intergenic
GGAGTGAGTCCACAAAACTG+GGG 0.660208 8.2:-24130117 None:intergenic
ATTCACTGCCAGAAGCACAA+GGG 0.669813 8.2:+24130919 MS.gene60412:CDS
AGAACAAAACAAGAAAACAA+AGG 0.679620 8.2:+24130741 MS.gene60412:CDS
ACTTGTCAAGAACCTACGCA+TGG 0.686710 8.2:+24132026 MS.gene60412:CDS
AGTGAGTCCACAAAACTGGG+GGG 0.773710 8.2:-24130115 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAGTATAGAAATAATTTAT+TGG + chr8.2:24131585-24131604 MS.gene60412:intron 15.0%
!! TCAAATACTGTTATAAAATT+TGG + chr8.2:24130641-24130660 MS.gene60412:intron 15.0%
!! TTTAAACAATGAATATTTAG+TGG - chr8.2:24131084-24131103 None:intergenic 15.0%
!!! AACTTGTTAAGAATAAAAAA+AGG + chr8.2:24131210-24131229 MS.gene60412:intron 15.0%
!!! AAGTATTATTATTTTGTTTG+AGG + chr8.2:24131139-24131158 MS.gene60412:intron 15.0%
!!! ATTAAATGCACAAAATTTTA+AGG + chr8.2:24131646-24131665 MS.gene60412:intron 15.0%
!!! ATTATTACATTTTTTGTCTA+GGG + chr8.2:24131373-24131392 MS.gene60412:intron 15.0%
!! AAGGAATTTATTACCATAAA+AGG + chr8.2:24131229-24131248 MS.gene60412:intron 20.0%
!! ATAATTAATGAAATGCTAAC+CGG - chr8.2:24131346-24131365 None:intergenic 20.0%
!! ATATTTAGTGGCAATTATTA+AGG - chr8.2:24131072-24131091 None:intergenic 20.0%
!!! AACTTGTGTTGTTTTTTTTT+TGG + chr8.2:24131522-24131541 MS.gene60412:intron 20.0%
!!! ATTGTTATACTTTTGTAGAT+GGG + chr8.2:24131976-24131995 MS.gene60412:intron 20.0%
!!! ATTTTTTTTTTTGTTGTTGC+AGG + chr8.2:24129753-24129772 MS.gene60412:intron 20.0%
!!! CACTACTTTTTTTTTATATC+AGG + chr8.2:24131721-24131740 MS.gene60412:intron 20.0%
!!! CATTATTACATTTTTTGTCT+AGG + chr8.2:24131372-24131391 MS.gene60412:intron 20.0%
!!! TATTGTTATACTTTTGTAGA+TGG + chr8.2:24131975-24131994 MS.gene60412:intron 20.0%
!!! TCTTTTTAGGAATTTTGTTT+AGG + chr8.2:24131884-24131903 MS.gene60412:intron 20.0%
!!! TGATTTGGTAAGAAAATTAA+AGG + chr8.2:24129676-24129695 MS.gene60412:intron 20.0%
!!! TTATTACATTTTTTGTCTAG+GGG + chr8.2:24131374-24131393 MS.gene60412:intron 20.0%
!!! TTTTCTATCTGTTTCTTTTT+AGG + chr8.2:24131871-24131890 MS.gene60412:intron 20.0%
! AATACAATTAAACAACTCGT+GGG - chr8.2:24131948-24131967 None:intergenic 25.0%
! AGAACAAAACAAGAAAACAA+AGG + chr8.2:24130741-24130760 MS.gene60412:CDS 25.0%
! AGTAGTGAAAAAATGTTTAG+TGG - chr8.2:24131115-24131134 None:intergenic 25.0%
! CTGTTATAAAATTTGGTAGA+TGG + chr8.2:24130648-24130667 MS.gene60412:intron 25.0%
! TAATACAATTAAACAACTCG+TGG - chr8.2:24131949-24131968 None:intergenic 25.0%
! TAATTAATGAAATGCTAACC+GGG - chr8.2:24131345-24131364 None:intergenic 25.0%
! TATTACACGTATTCATGAAA+GGG + chr8.2:24131825-24131844 MS.gene60412:intron 25.0%
! TTATTACACGTATTCATGAA+AGG + chr8.2:24131824-24131843 MS.gene60412:intron 25.0%
!! TAAGGAATACTTCTTCTTTT+GGG + chr8.2:24131664-24131683 MS.gene60412:intron 25.0%
!! TGTTGGCTGTTATAATTAAT+TGG - chr8.2:24131780-24131799 None:intergenic 25.0%
!! TTAAGGAATACTTCTTCTTT+TGG + chr8.2:24131663-24131682 MS.gene60412:intron 25.0%
!!! AAAGTATCGTCAATAATTGT+TGG - chr8.2:24131797-24131816 None:intergenic 25.0%
!!! AATAATTAACTTGTGCCTTA+AGG - chr8.2:24131565-24131584 None:intergenic 25.0%
!!! ATAATTAACTTGTGCCTTAA+GGG - chr8.2:24131564-24131583 None:intergenic 25.0%
AAAGATGAGTTAGATGATGA+TGG - chr8.2:24130042-24130061 None:intergenic 30.0%
AATATCATTAATCAATGCCC+CGG + chr8.2:24131324-24131343 MS.gene60412:intron 30.0%
AATTAATGAAATGCTAACCG+GGG - chr8.2:24131344-24131363 None:intergenic 30.0%
ACATGAATGTTTCTCATTTG+GGG - chr8.2:24130192-24130211 None:intergenic 30.0%
AGACATGAATGTTTCTCATT+TGG - chr8.2:24130194-24130213 None:intergenic 30.0%
AGCAATGATAGTAATGTCAA+TGG - chr8.2:24131025-24131044 None:intergenic 30.0%
ATTATTAGGGTTATGCTAAC+CGG + chr8.2:24131177-24131196 MS.gene60412:intron 30.0%
CAAAAACATCTCAAACCAAA+AGG + chr8.2:24130259-24130278 MS.gene60412:CDS 30.0%
CATCATCATCAATGATACTA+TGG - chr8.2:24130798-24130817 None:intergenic 30.0%
GACATGAATGTTTCTCATTT+GGG - chr8.2:24130193-24130212 None:intergenic 30.0%
TAAAATTTGGTAGATGGACA+AGG + chr8.2:24130654-24130673 MS.gene60412:intron 30.0%
TCTCAACTCAAAATCCTAAA+GGG + chr8.2:24130211-24130230 MS.gene60412:CDS 30.0%
TTAGTGGCAATTATTAAGGT+AGG - chr8.2:24131068-24131087 None:intergenic 30.0%
! AAGGAATACTTCTTCTTTTG+GGG + chr8.2:24131665-24131684 MS.gene60412:intron 30.0%
! GTTAACGAAGTTTCCTTTTA+TGG - chr8.2:24131245-24131264 None:intergenic 30.0%
! TGAACTCAAACATTGTTGTT+GGG - chr8.2:24129866-24129885 None:intergenic 30.0%
!! TCATATGATGAGAGTATTGA+AGG + chr8.2:24130835-24130854 MS.gene60412:CDS 30.0%
!!! AACATTGATTTTGAAAGCGA+GGG - chr8.2:24131421-24131440 None:intergenic 30.0%
!!! ACTTTTGACTCTTTTGACTT+TGG + chr8.2:24129931-24129950 MS.gene60412:CDS 30.0%
!!! CTGTTTTGACTTTGAGTTTA+AGG - chr8.2:24130145-24130164 None:intergenic 30.0%
!!! TAAAAAGCGTTTGGAAGTTT+TGG + chr8.2:24130717-24130736 MS.gene60412:CDS 30.0%
!!! TGTTGCAAAGTTTTGAGTTT+TGG - chr8.2:24130241-24130260 None:intergenic 30.0%
!!! TTTTTTAACATGTGCCCTAA+GGG + chr8.2:24131688-24131707 MS.gene60412:intron 30.0%
!!! TTTTTTGTTGTTGCAGGAAA+TGG + chr8.2:24129759-24129778 MS.gene60412:intron 30.0%
AAACTTCCTTGATGCTAATG+AGG + chr8.2:24129816-24129835 MS.gene60412:CDS 35.0%
AATGTGTATTAGTGTCGTGT+TGG - chr8.2:24130446-24130465 None:intergenic 35.0%
CACTTCACACTTATGTTTGA+AGG + chr8.2:24130391-24130410 MS.gene60412:intron 35.0%
CATTAGCATCAAGGAAGTTT+AGG - chr8.2:24129816-24129835 None:intergenic 35.0%
CTGAACTCAAACATTGTTGT+TGG - chr8.2:24129867-24129886 None:intergenic 35.0%
GTCTCAACTCAAAATCCTAA+AGG + chr8.2:24130210-24130229 MS.gene60412:CDS 35.0%
GTTTCTTCTGATACGTTCAT+TGG - chr8.2:24129641-24129660 None:intergenic 35.0%
TAACAAGTTTCTCAGAGTAC+CGG - chr8.2:24131199-24131218 None:intergenic 35.0%
TACTATCATTGCTCAGGTAA+AGG + chr8.2:24131032-24131051 MS.gene60412:intron 35.0%
TATCAGAAGAAACCAGCAAA+TGG + chr8.2:24129648-24129667 MS.gene60412:CDS 35.0%
TCATCAATGATACTATGGTC+TGG - chr8.2:24130793-24130812 None:intergenic 35.0%
TCTTGTTCCTAACCTGAAAA+AGG + chr8.2:24130365-24130384 MS.gene60412:CDS 35.0%
TGACATTACTATCATTGCTC+AGG + chr8.2:24131026-24131045 MS.gene60412:CDS 35.0%
TGTTTCATCAGTGAATGAGT+TGG - chr8.2:24129911-24129930 None:intergenic 35.0%
! AACAATTGTTTTCAGCTCCA+TGG - chr8.2:24129977-24129996 None:intergenic 35.0%
! ATACTTTTGTAGATGGGTGA+AGG + chr8.2:24131982-24132001 MS.gene60412:intron 35.0%
! TACTTTTGTAGATGGGTGAA+GGG + chr8.2:24131983-24132002 MS.gene60412:intron 35.0%
! TTTGACTTTGAGTTTAAGGC+AGG - chr8.2:24130141-24130160 None:intergenic 35.0%
!! AAGTTTAGGTTGCATTCTTC+AGG - chr8.2:24129802-24129821 None:intergenic 35.0%
!! AATGTTAACTTGTGCCCTTA+AGG + chr8.2:24131547-24131566 MS.gene60412:intron 35.0%
!! GAAAGAGCTTAAAAAGCGTT+TGG + chr8.2:24130708-24130727 MS.gene60412:CDS 35.0%
!! TGGAGAAAGTTTTGGTGAAA+TGG - chr8.2:24130022-24130041 None:intergenic 35.0%
!! TGTGAAGTGTTACCTTTTTC+AGG - chr8.2:24130380-24130399 None:intergenic 35.0%
!! TTGAGTGCTATTTAGCTAGT+GGG - chr8.2:24130576-24130595 None:intergenic 35.0%
!! TTTGAGTGCTATTTAGCTAG+TGG - chr8.2:24130577-24130596 None:intergenic 35.0%
!!! AGTGTTACCTTTTTCAGGTT+AGG - chr8.2:24130375-24130394 None:intergenic 35.0%
!!! CAACATTGATTTTGAAAGCG+AGG - chr8.2:24131422-24131441 None:intergenic 35.0%
!!! GATGATGATGGAGAAAGTTT+TGG - chr8.2:24130030-24130049 None:intergenic 35.0%
!!! GTTTTTTAACATGTGCCCTA+AGG + chr8.2:24131687-24131706 MS.gene60412:intron 35.0%
!!! TGAGTTTTGGATAACCCTTT+AGG - chr8.2:24130228-24130247 None:intergenic 35.0%
AATAGCAGTGTCTTACCCTT+TGG + chr8.2:24130994-24131013 MS.gene60412:CDS 40.0%
AATGTCAATGGAATCCCCAA+AGG - chr8.2:24131013-24131032 None:intergenic 40.0%
ACACGTATTCATGAAAGGGA+GGG + chr8.2:24131829-24131848 MS.gene60412:intron 40.0%
ACTCTTCCTCATTAGCATCA+AGG - chr8.2:24129825-24129844 None:intergenic 40.0%
AGGGTACAACTTGAGCATAA+AGG + chr8.2:24132002-24132021 MS.gene60412:CDS 40.0%
ATAGCAGTGTCTTACCCTTT+GGG + chr8.2:24130995-24131014 MS.gene60412:CDS 40.0%
ATGTCAATGGAATCCCCAAA+GGG - chr8.2:24131012-24131031 None:intergenic 40.0%
CAAATCAGAAAGCCATTTGC+TGG - chr8.2:24129663-24129682 None:intergenic 40.0%
CAGCAAATGGCTTTCTGATT+TGG + chr8.2:24129661-24129680 MS.gene60412:CDS 40.0%
GCTTTCTTTCAGCCATGATA+TGG - chr8.2:24130305-24130324 None:intergenic 40.0%
TACACGTATTCATGAAAGGG+AGG + chr8.2:24131828-24131847 MS.gene60412:intron 40.0%
TGAAATTGAGAGACCAACCA+TGG + chr8.2:24129957-24129976 MS.gene60412:CDS 40.0%
TGAATGTTTCTCATTTGGGG+TGG - chr8.2:24130189-24130208 None:intergenic 40.0%
TGGTTTCTTCAGAACAACCA+CGG - chr8.2:24130773-24130792 None:intergenic 40.0%
TTACTGTCGATGTTGTGTCT+TGG + chr8.2:24130515-24130534 MS.gene60412:intron 40.0%
TTCAGCCATGATATGGTCAT+CGG - chr8.2:24130298-24130317 None:intergenic 40.0%
! ATTGTTTTCAGCTCCATGGT+TGG - chr8.2:24129973-24129992 None:intergenic 40.0%
! CACTTTTGCTTGCACTTGAA+AGG - chr8.2:24130875-24130894 None:intergenic 40.0%
! TGTTGGTAGGAGGAGAATTT+GGG - chr8.2:24130095-24130114 None:intergenic 40.0%
! TGTTGTTGGGTTTGTTCTTG+TGG - chr8.2:24129853-24129872 None:intergenic 40.0%
!! AAGTGCAAGCAAAAGTGTCA+GGG + chr8.2:24130878-24130897 MS.gene60412:CDS 40.0%
!! AGTGTTAACTTGTGCCCTTA+GGG - chr8.2:24131705-24131724 None:intergenic 40.0%
!! GAATGAGTTGGTGATAGTTG+TGG - chr8.2:24129899-24129918 None:intergenic 40.0%
!! GTTGGTGATAGTTGTGGAAT+TGG - chr8.2:24129893-24129912 None:intergenic 40.0%
!! TAGTGTTAACTTGTGCCCTT+AGG - chr8.2:24131706-24131725 None:intergenic 40.0%
!! TGAGTGCTATTTAGCTAGTG+GGG - chr8.2:24130575-24130594 None:intergenic 40.0%
ACTTGTCAAGAACCTACGCA+TGG + chr8.2:24132026-24132045 MS.gene60412:CDS 45.0%
AGGAGTGAGTCCACAAAACT+GGG - chr8.2:24130121-24130140 None:intergenic 45.0%
CTGCAAGAGCAATGAAGCTT+TGG - chr8.2:24130344-24130363 None:intergenic 45.0%
GAAAACAAAGGTAGAGTCCG+TGG + chr8.2:24130753-24130772 MS.gene60412:CDS 45.0%
TAGCAGTGTCTTACCCTTTG+GGG + chr8.2:24130996-24131015 MS.gene60412:CDS 45.0%
! ATTCACTGCCAGAAGCACAA+GGG + chr8.2:24130919-24130938 MS.gene60412:CDS 45.0%
! CAGGGAAAGAGATGCTGATT+CGG + chr8.2:24130896-24130915 MS.gene60412:CDS 45.0%
! GTGTTGGTAGGAGGAGAATT+TGG - chr8.2:24130096-24130115 None:intergenic 45.0%
!! CAAGTGCAAGCAAAAGTGTC+AGG + chr8.2:24130877-24130896 MS.gene60412:CDS 45.0%
!! GGGAGGGCGTATTTTATTCT+AGG + chr8.2:24131845-24131864 MS.gene60412:intron 45.0%
!! ACTAATTATTATTAATTATT+AGG + chr8.2:24131163-24131182 MS.gene60412:intron 5.0%
!! CTAATTATTATTAATTATTA+GGG + chr8.2:24131164-24131183 MS.gene60412:intron 5.0%
ACTTCAATGCTGCCATGCGT+AGG - chr8.2:24132041-24132060 None:intergenic 50.0%
AGTGAGTCCACAAAACTGGG+GGG - chr8.2:24130118-24130137 None:intergenic 50.0%
CAACGCCGATGACCATATCA+TGG + chr8.2:24130290-24130309 MS.gene60412:CDS 50.0%
CAGGAGTGAGTCCACAAAAC+TGG - chr8.2:24130122-24130141 None:intergenic 50.0%
CATGATATGGTCATCGGCGT+TGG - chr8.2:24130292-24130311 None:intergenic 50.0%
GAGTGAGTCCACAAAACTGG+GGG - chr8.2:24130119-24130138 None:intergenic 50.0%
GGAGTGAGTCCACAAAACTG+GGG - chr8.2:24130120-24130139 None:intergenic 50.0%
GGGGATTCTGGTGAAAGTCA+TGG + chr8.2:24130939-24130958 MS.gene60412:CDS 50.0%
GGTCATCTGAGTGACACGTT+TGG - chr8.2:24131400-24131419 None:intergenic 50.0%
TGGTAGGAGGAGAATTTGGG+AGG - chr8.2:24130092-24130111 None:intergenic 50.0%
! GATTCACTGCCAGAAGCACA+AGG + chr8.2:24130918-24130937 MS.gene60412:CDS 50.0%
! TTCACTGCCAGAAGCACAAG+GGG + chr8.2:24130920-24130939 MS.gene60412:CDS 50.0%
CCAGAATCCCCTTGTGCTTC+TGG - chr8.2:24130930-24130949 None:intergenic 55.0%
! ACCAACACCCCCCAGTTTTG+TGG + chr8.2:24130108-24130127 MS.gene60412:CDS 55.0%
! CCAGAAGCACAAGGGGATTC+TGG + chr8.2:24130927-24130946 MS.gene60412:CDS 55.0%
! TCCACAAAACTGGGGGGTGT+TGG - chr8.2:24130112-24130131 None:intergenic 55.0%
!! CAAAACTGGGGGGTGTTGGT+AGG - chr8.2:24130108-24130127 None:intergenic 55.0%
!! AACTGGGGGGTGTTGGTAGG+AGG - chr8.2:24130105-24130124 None:intergenic 60.0%
!!! GGCGTTGGCTCGAGACCTTT+TGG - chr8.2:24130277-24130296 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 24129617 24132083 24129617 ID=MS.gene60412
chr8.2 mRNA 24129617 24132083 24129617 ID=MS.gene60412.t1;Parent=MS.gene60412
chr8.2 exon 24129617 24129682 24129617 ID=MS.gene60412.t1.exon1;Parent=MS.gene60412.t1
chr8.2 CDS 24129617 24129682 24129617 ID=cds.MS.gene60412.t1;Parent=MS.gene60412.t1
chr8.2 exon 24129775 24130386 24129775 ID=MS.gene60412.t1.exon2;Parent=MS.gene60412.t1
chr8.2 CDS 24129775 24130386 24129775 ID=cds.MS.gene60412.t1;Parent=MS.gene60412.t1
chr8.2 exon 24130667 24131047 24130667 ID=MS.gene60412.t1.exon3;Parent=MS.gene60412.t1
chr8.2 CDS 24130667 24131047 24130667 ID=cds.MS.gene60412.t1;Parent=MS.gene60412.t1
chr8.2 exon 24131994 24132083 24131994 ID=MS.gene60412.t1.exon4;Parent=MS.gene60412.t1
chr8.2 CDS 24131994 24132083 24131994 ID=cds.MS.gene60412.t1;Parent=MS.gene60412.t1
Gene Sequence

>MS.gene60412

ATGGAAGAAATCAACAACACACCAATGAACGTATCAGAAGAAACCAGCAAATGGCTTTCTGATTTGGTAAGAAAATTAAAGGCAAATTAACATATTATTGATTATATAAGTAAATTCTTTATCATTTTAAATTTTAATTTTTTTTTTTGTTGTTGCAGGAAATGGATGAGTATAATTTGTTTCCTGAAGAATGCAACCTAAACTTCCTTGATGCTAATGAGGAAGAGTTTCTTCCACAAGAACAAACCCAACAACAATGTTTGAGTTCAGAGTCCAATTCCACAACTATCACCAACTCATTCACTGATGAAACAACTTTTGACTCTTTTGACTTTGGTTTTGAAATTGAGAGACCAACCATGGAGCTGAAAACAATTGTTAGTGACAACAACATCATTGAAACCATTTCACCAAAACTTTCTCCATCATCATCTAACTCATCTTTGCACTCTCAGATTTTGTCTTTTGACAACCTCCCAAATTCTCCTCCTACCAACACCCCCCAGTTTTGTGGACTCACTCCTGCCTTAAACTCAAAGTCAAAACAGAACAAAACAGTGTCAGTGTTTCCACCCCAAATGAGAAACATTCATGTCTCAACTCAAAATCCTAAAGGGTTATCCAAAACTCAAAACTTTGCAACAAAAACATCTCAAACCAAAAGGTCTCGAGCCAACGCCGATGACCATATCATGGCTGAAAGAAAGCGAAGAGAGAAACTTAGCCAAAGCTTCATTGCTCTTGCAGCTCTTGTTCCTAACCTGAAAAAGGTAACACTTCACACTTATGTTTGAAGGCGTGTCTCATGTTTGTCACGTATCAGTGTCCAACACGACACTAATACACATTATTACATACAATAATGTCAATTTTACGAATAATTAGTAATATTGACATGTTACTGTCGATGTTGTGTCTTGGTGTCTGTGTTAAGACAATAAATCAATTTTGATTACCCCACTAGCTAAATAGCACTCAAATCATTGTATTTTTATTTTCTTCATGAATTGTTAACATCTTCTGATCAAATACTGTTATAAAATTTGGTAGATGGACAAGGCTTCTGTATTAGCTGAGTCTATCACATACGTGAAAGAGCTTAAAAAGCGTTTGGAAGTTTTGGAAGAACAAAACAAGAAAACAAAGGTAGAGTCCGTGGTTGTTCTGAAGAAACCAGACCATAGTATCATTGATGATGATGATGATGATGATAGCTCATCATATGATGAGAGTATTGAAGGTGCTACTGATTCATCCTTTCAAGTGCAAGCAAAAGTGTCAGGGAAAGAGATGCTGATTCGGATTCACTGCCAGAAGCACAAGGGGATTCTGGTGAAAGTCATGGCTGAGATTCAAAGCATACAATTGTTTGCTGTGAATAGCAGTGTCTTACCCTTTGGGGATTCCATTGACATTACTATCATTGCTCAGGTAAAGGACAATCTCTACCTACCTTAATAATTGCCACTAAATATTCATTGTTTAAAAGAGAATGCCACTAAACATTTTTTCACTACTTTTTAAGTATTATTATTTTGTTTGAGGCACTAATTATTATTAATTATTAGGGTTATGCTAACCGGTACTCTGAGAAACTTGTTAAGAATAAAAAAAGGAATTTATTACCATAAAAGGAAACTTCGTTAACTTATTTATGAATTAATTACACAGTTTTCAATGCAATAATTACTATATAATTTTCTTTTTAATATCATTAATCAATGCCCCGGTTAGCATTTCATTAATTATTATTATCATTATTACATTTTTTGTCTAGGGGCCAAACGTGTCACTCAGATGACCCTCGCTTTCAAAATCAATGTTGTTTTAGTCTAAAAAATTAGAATAAGTGTTGTTTTATTTTTCTATTGTAGTGTTAGTCAATTTATTTTAATTTTACTATTAAACTTGTGTTGTTTTTTTTTTGGAAAATGTTAACTTGTGCCCTTAAGGCACAAGTTAATTATTGTAGTATAGAAATAATTTATTGGAGTGTGTGCAATGTATTTAATTTTTAACTTTTTAGTCAATTAAATGCACAAAATTTTAAGGAATACTTCTTCTTTTGGGGTTTTTTAACATGTGCCCTAAGGGCACAAGTTAACACTACTTTTTTTTTATATCAGGATGTTAAATTTATGTTGCAATACATCATCACTTCCAATTAATTATAACAGCCAACAATTATTGACGATACTTTTAAAAAATTATTACACGTATTCATGAAAGGGAGGGCGTATTTTATTCTAGGCTGTTTTCTATCTGTTTCTTTTTAGGAATTTTGTTTAGGAGTATCTTTTTTTTTTATTATTATTTTTGTCTTATTTGCCCACGAGTTGTTTAATTGTATTAAAATACTATTGTTATACTTTTGTAGATGGGTGAAGGGTACAACTTGAGCATAAAGGAACTTGTCAAGAACCTACGCATGGCAGCATTGAAGTTTATGTCATCAAAGCATGAATGA
Protein sequence

>MS.gene60412.t1

MEEINNTPMNVSEETSKWLSDLEMDEYNLFPEECNLNFLDANEEEFLPQEQTQQQCLSSESNSTTITNSFTDETTFDSFDFGFEIERPTMELKTIVSDNNIIETISPKLSPSSSNSSLHSQILSFDNLPNSPPTNTPQFCGLTPALNSKSKQNKTVSVFPPQMRNIHVSTQNPKGLSKTQNFATKTSQTKRSRANADDHIMAERKRREKLSQSFIALAALVPNLKKMDKASVLAESITYVKELKKRLEVLEEQNKKTKVESVVVLKKPDHSIIDDDDDDDSSSYDESIEGATDSSFQVQAKVSGKEMLIRIHCQKHKGILVKVMAEIQSIQLFAVNSSVLPFGDSIDITIIAQMGEGYNLSIKELVKNLRMAALKFMSSKHE