AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60486

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60486.t1 MTR_3g029590 97.275 734 15 4 1 732 1 731 0.0 1457
MS.gene60486.t1 MTR_7g092070 64.067 718 219 17 33 731 33 730 0.0 831
MS.gene60486.t1 MTR_0728s0020 58.039 622 227 12 29 642 13 608 0.0 635
MS.gene60486.t1 MTR_1g050438 58.049 615 224 12 33 639 1 589 0.0 629
MS.gene60486.t1 MTR_3g435590 63.783 497 101 4 1 497 1 418 0.0 604
MS.gene60486.t1 MTR_3g435590 51.087 92 43 2 591 682 379 468 5.92e-18 87.8
MS.gene60486.t1 MTR_3g435680 62.408 407 89 9 95 497 5 351 2.57e-152 448
MS.gene60486.t1 MTR_5g082830 40.865 717 326 22 45 729 28 678 2.63e-145 441
MS.gene60486.t1 MTR_5g071070 39.208 732 333 19 45 729 33 699 8.31e-141 430
MS.gene60486.t1 MTR_5g071070 39.003 682 304 19 95 729 1 617 6.23e-126 389
MS.gene60486.t1 MTR_1g075890 38.268 635 277 17 44 668 30 559 8.75e-121 376
MS.gene60486.t1 MTR_3g070880 34.342 661 244 17 45 691 8 492 3.91e-104 328
MS.gene60486.t1 MTR_3g464260 42.651 347 126 8 52 392 30 309 1.35e-76 256
MS.gene60486.t1 MTR_3g464280 34.752 423 164 11 49 457 19 343 6.12e-59 207
MS.gene60486.t1 MTR_6g090380 72.897 107 29 0 262 368 99 205 1.63e-55 195
MS.gene60486.t1 MTR_8g102100 52.410 166 68 2 262 420 77 238 5.33e-52 185
MS.gene60486.t1 MTR_2g090605 67.568 111 36 0 266 376 77 187 8.33e-52 186
MS.gene60486.t1 MTR_3g103960 62.281 114 42 1 256 368 43 156 1.23e-48 173
MS.gene60486.t1 MTR_4g078660 61.682 107 41 0 262 368 56 162 2.12e-47 171
MS.gene60486.t1 MTR_3g464270 32.483 431 196 15 265 682 8 356 3.07e-46 169
MS.gene60486.t1 MTR_3g464290 31.602 462 211 16 245 687 79 454 2.21e-40 155
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60486.t1 AT2G41900 59.157 759 235 24 1 731 1 712 0.0 772
MS.gene60486.t1 AT5G12850 52.727 715 268 23 41 729 36 706 0.0 667
MS.gene60486.t1 AT5G58620 41.515 660 283 18 44 685 11 585 2.58e-139 423
MS.gene60486.t1 AT2G40140 38.056 720 292 22 26 729 10 591 2.44e-125 386
MS.gene60486.t1 AT2G40140 38.056 720 292 22 26 729 10 591 2.44e-125 386
MS.gene60486.t1 AT3G55980 37.920 654 258 18 47 686 97 616 9.84e-117 365
MS.gene60486.t1 AT3G55980 37.867 647 268 17 47 686 30 549 1.22e-115 360
MS.gene60486.t1 AT2G19810 68.033 122 37 1 251 370 61 182 2.45e-55 194
MS.gene60486.t1 AT4G29190 53.939 165 70 1 255 413 68 232 8.62e-55 192
MS.gene60486.t1 AT2G25900 69.725 109 33 0 262 370 86 194 1.12e-54 191
MS.gene60486.t1 AT2G25900 69.725 109 33 0 262 370 86 194 1.12e-54 191
MS.gene60486.t1 AT5G07500 61.983 121 46 0 248 368 34 154 5.51e-53 184
MS.gene60486.t1 AT1G03790 54.601 163 66 3 223 381 43 201 2.64e-52 186
MS.gene60486.t1 AT5G44260 61.719 128 44 2 244 370 42 165 8.40e-49 176

Find 187 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTTATCATCTGCTGAAAA+TGG 0.161392 3.2:-20225615 MS.gene60486:CDS
ACAGCTGAGTCGGTAAATTT+TGG 0.186788 3.2:+20225570 None:intergenic
CGGCATTGATCACTCTGTTT+TGG 0.190245 3.2:-20224131 MS.gene60486:CDS
CAGCTGTAGTGAAACTTTCT+AGG 0.248659 3.2:+20225360 None:intergenic
TAGGAAGCAGCCACCATTAA+AGG 0.251482 3.2:+20225987 None:intergenic
TGAGCACCGAACTCCTTTAA+TGG 0.259664 3.2:-20226000 MS.gene60486:CDS
CTAGTTTGATTAATGCTTCC+TGG 0.298175 3.2:+20224946 None:intergenic
CTTCTAGGCGACATCCTTCC+AGG 0.313255 3.2:+20224532 None:intergenic
AACCAGGACTCGATGAAATA+AGG 0.317452 3.2:+20225646 None:intergenic
CACTTAGACCAGGAGTTAAC+AGG 0.326627 3.2:+20224373 None:intergenic
CATACTGAGCCTGGTGGAAT+AGG 0.327090 3.2:+20224566 None:intergenic
TTCGGTGCTCAAGAACAATT+TGG 0.328148 3.2:+20226012 None:intergenic
CCCTCTCTCCAATGAGTTCT+CGG 0.331992 3.2:-20224501 MS.gene60486:CDS
TTATTAATGAAGTTGGCTTC+TGG 0.334337 3.2:-20226058 MS.gene60486:CDS
TGTGTTTGACCGAAGTCATT+TGG 0.347057 3.2:+20224310 None:intergenic
GCAAGCTTCTCTTTCTTCTC+AGG 0.349206 3.2:+20224205 None:intergenic
ATCAATTCGGTGGATGCTAA+TGG 0.355260 3.2:-20225804 MS.gene60486:CDS
TACAGCGGACGCAGCTCTTC+AGG 0.358359 3.2:+20225174 None:intergenic
AAAGAGAAGCTTGCAGGTTC+CGG 0.358491 3.2:-20224196 MS.gene60486:CDS
GAGAGGGCTTCCATACTTCT+AGG 0.360535 3.2:+20224517 None:intergenic
GAGAAGAAAGAGAAGCTTGC+AGG 0.368507 3.2:-20224202 MS.gene60486:CDS
ATACTGAGCCTGGTGGAATA+GGG 0.368901 3.2:+20224567 None:intergenic
TTCTCTTTCTTCTCAGGTGT+GGG 0.372072 3.2:+20224211 None:intergenic
GATATGAGAGTATAAGCTTT+AGG 0.383249 3.2:+20225949 None:intergenic
AAGGATGTCGCCTAGAAGTA+TGG 0.388115 3.2:-20224527 MS.gene60486:CDS
AATAGATCATCAAGATTTGA+TGG 0.389042 3.2:+20224751 None:intergenic
ATGCCGTCCACTTGAGGGTT+GGG 0.394038 3.2:+20224148 None:intergenic
AACAATCCTGTCTCAGCTGT+TGG 0.394825 3.2:-20224400 MS.gene60486:CDS
TTAATGGTGGCTGCTTCCTA+TGG 0.398871 3.2:-20225984 MS.gene60486:CDS
AGCTTTGAAGCTGTTGAAAC+TGG 0.398962 3.2:+20224641 None:intergenic
GAAATCTTCAAAATCATTGT+TGG 0.399103 3.2:+20226109 None:intergenic
AGCAGTAGAAGCAGCTGATC+GGG 0.402586 3.2:+20225122 None:intergenic
CCATTTGCAGATGGGGATAG+AGG 0.407663 3.2:+20225012 None:intergenic
AGGGGATGCTCTACGTTTGT+AGG 0.408542 3.2:+20224586 None:intergenic
GCTCTTCTCCATTGTTTCCA+TGG 0.413958 3.2:+20224269 None:intergenic
GAGATTGATGATGGTGAACC+AGG 0.414210 3.2:+20225063 None:intergenic
CTTCTCTTTCTTCTCAGGTG+TGG 0.414946 3.2:+20224210 None:intergenic
GCTTCACTTATTAATGAAGT+TGG 0.420259 3.2:-20226065 MS.gene60486:CDS
ATTCCGCATGTATTCATTCA+AGG 0.421704 3.2:-20225466 MS.gene60486:CDS
TCAGGAAGAGATGGGTCAAC+TGG 0.426785 3.2:+20225519 None:intergenic
CAGTATGGTCTCTCTTCTCC+TGG 0.430222 3.2:-20224550 MS.gene60486:CDS
AGCCTTGTCCGATACTGAGC+TGG 0.430472 3.2:+20225249 None:intergenic
GCTATTTCAGTCGGTCGATC+TGG 0.432976 3.2:-20224793 MS.gene60486:CDS
ACAATCCTGTCTCAGCTGTT+GGG 0.435810 3.2:-20224399 MS.gene60486:CDS
AATATGTTGCAAGACTTTGA+TGG 0.442806 3.2:-20224868 MS.gene60486:CDS
TGTTTCTCACAGCCCCGTCC+TGG 0.446692 3.2:-20224817 MS.gene60486:CDS
TTCTCTCCAGGATGCACAAA+AGG 0.447031 3.2:+20225399 None:intergenic
CTTCTGGTATGTCCGTCAAA+AGG 0.447129 3.2:-20226042 MS.gene60486:CDS
CTCCTATTGGAAAAGCTGAT+TGG 0.449350 3.2:-20224342 MS.gene60486:CDS
TCAATTCGGTGGATGCTAAT+GGG 0.451393 3.2:-20225803 MS.gene60486:CDS
AAATGAAGAGCTGGCACATT+GGG 0.453972 3.2:+20224970 None:intergenic
AGCTTATGGGGAACAACAAT+AGG 0.455127 3.2:+20225753 None:intergenic
AGACATGACATTTGTGTTGA+TGG 0.455769 3.2:+20224609 None:intergenic
AAAAGGGGCTTGCAGACGTT+CGG 0.457342 3.2:-20225316 MS.gene60486:CDS
TTGGGTCCCCTGTTAACTCC+TGG 0.457724 3.2:-20224381 MS.gene60486:CDS
AGACAGCAGATTTGTGTGTT+GGG 0.461702 3.2:+20224668 None:intergenic
TTGGCTTGAGCAACTGCAGC+TGG 0.464022 3.2:-20224104 MS.gene60486:CDS
CTGTGCTGCCTCAAGTGGTT+CGG 0.464047 3.2:-20225874 MS.gene60486:CDS
CTCCTTCGAGCATTCTCTCC+AGG 0.464846 3.2:+20225387 None:intergenic
TCCCTTATTTCATCGAGTCC+TGG 0.465549 3.2:-20225648 MS.gene60486:CDS
AAGGCTCTGCAAAGACGGTA+TGG 0.467673 3.2:-20225232 MS.gene60486:CDS
TCCTCTTCATCTGCGGCTGA+AGG 0.470214 3.2:-20226215 MS.gene60486:CDS
CGTCCGCTGTATGTGTCCAC+TGG 0.470219 3.2:-20225162 MS.gene60486:CDS
GACTGAAATAGCACCAGGAC+GGG 0.471789 3.2:+20224804 None:intergenic
CTTTATCATCTGCTGAAAAT+GGG 0.474367 3.2:-20225614 MS.gene60486:CDS
CTGTTAACTCCTGGTCTAAG+TGG 0.474677 3.2:-20224372 MS.gene60486:CDS
GAAAATACAGAAGCCGCGGC+AGG 0.477087 3.2:+20224691 None:intergenic
TAATGGTGGCTGCTTCCTAT+GGG 0.478863 3.2:-20225983 MS.gene60486:CDS
ATTTGTGTTGATGGGTGATA+AGG 0.483158 3.2:+20224618 None:intergenic
CTCATGGGGTATTTGAGTGC+TGG 0.484149 3.2:-20225278 MS.gene60486:CDS
TTCTGGTATGTCCGTCAAAA+GGG 0.484629 3.2:-20226041 MS.gene60486:CDS
TGTTAACTCCTGGTCTAAGT+GGG 0.486046 3.2:-20224371 MS.gene60486:CDS
CCTCTATCCCCATCTGCAAA+TGG 0.492687 3.2:-20225012 MS.gene60486:CDS
GCATTATTGCCATTTGCAGA+TGG 0.493990 3.2:+20225003 None:intergenic
CATTATTGCCATTTGCAGAT+GGG 0.495177 3.2:+20225004 None:intergenic
ACCAGGACTCGATGAAATAA+GGG 0.495790 3.2:+20225647 None:intergenic
GATCAATGCCGTCCACTTGA+GGG 0.496520 3.2:+20224143 None:intergenic
ATGATGGTGAACCAGGGAAA+AGG 0.496915 3.2:+20225070 None:intergenic
GGAAGCATGTTCGAGTAAGC+TGG 0.497010 3.2:+20226130 None:intergenic
CTATTCCACCAGGCTCAGTA+TGG 0.498689 3.2:-20224565 MS.gene60486:CDS
GGAACAACAATAACATCGAC+AGG 0.499757 3.2:+20225774 None:intergenic
CTTCACTGTGCTGCCTCAAG+TGG 0.502797 3.2:-20225879 MS.gene60486:CDS
AGAAGAGCCTGATGTAGGTT+GGG 0.503303 3.2:-20224254 MS.gene60486:CDS
TTTGGAGCCACAGGAGAGGA+TGG 0.503694 3.2:+20225588 None:intergenic
GCTGACAACCGAGAACTCAT+TGG 0.504299 3.2:+20224493 None:intergenic
AGCTGGTGCTGATATCAATT+CGG 0.504695 3.2:-20225817 MS.gene60486:CDS
CTCGTGCATACTCTCATGAT+TGG 0.506954 3.2:-20225431 MS.gene60486:CDS
CTGCAAATGGCAATAATGCT+TGG 0.507435 3.2:-20224999 MS.gene60486:CDS
CTTCCTGGTAAATGAAGAGC+TGG 0.509476 3.2:+20224961 None:intergenic
AGCCTTAGCTCAAGAGAACT+TGG 0.510474 3.2:-20224427 MS.gene60486:CDS
CACCAAGTTCTCTTGAGCTA+AGG 0.512371 3.2:+20224425 None:intergenic
CAGCCACCATTAAAGGAGTT+CGG 0.512886 3.2:+20225994 None:intergenic
GAGAAGAGCCTGATGTAGGT+TGG 0.517531 3.2:-20224255 MS.gene60486:CDS
TGATCAATGCCGTCCACTTG+AGG 0.517576 3.2:+20224142 None:intergenic
GTGCCAGCTCTTCATTTACC+AGG 0.519169 3.2:-20224964 MS.gene60486:CDS
GAATGGACCCAACCTACATC+AGG 0.520252 3.2:+20224247 None:intergenic
AAGACAGCAGATTTGTGTGT+TGG 0.520972 3.2:+20224667 None:intergenic
GCCTTGTCCGATACTGAGCT+GGG 0.524492 3.2:+20225250 None:intergenic
ATTGTTTCCATGGTCAAAAG+AGG 0.526082 3.2:+20224279 None:intergenic
AAGAGAGACCATACTGAGCC+TGG 0.528223 3.2:+20224557 None:intergenic
GGGGATAGAGGCTGTGCAAA+GGG 0.528233 3.2:+20225024 None:intergenic
CTGAGATTTCTTCATCCCCT+CGG 0.532952 3.2:-20224723 MS.gene60486:CDS
ATATGTTGCAAGACTTTGAT+GGG 0.533657 3.2:-20224867 MS.gene60486:CDS
TTGGGTCCATTCCCTTGTCA+AGG 0.537395 3.2:-20224236 MS.gene60486:CDS
CTCGATGAAATAAGGGACAT+TGG 0.538353 3.2:+20225654 None:intergenic
ATTATTGCCATTTGCAGATG+GGG 0.539077 3.2:+20225005 None:intergenic
GGGGACCCAACAGCTGAGAC+AGG 0.540557 3.2:+20224394 None:intergenic
ACCTTCAGCCGCAGATGAAG+AGG 0.541690 3.2:+20226214 None:intergenic
CATCATGTAGCGGCATATCA+CGG 0.544466 3.2:+20224899 None:intergenic
GGCTACACCCAGCTCAGTAT+CGG 0.547166 3.2:-20225257 MS.gene60486:CDS
TAGCAGTAGAAGCAGCTGAT+CGG 0.547990 3.2:+20225121 None:intergenic
TTCAGCGGATGGAATTGGGC+CGG 0.548143 3.2:+20224177 None:intergenic
ATCATGTAGCGGCATATCAC+GGG 0.549825 3.2:+20224900 None:intergenic
GATATGTGTGAATATGCTCA+TGG 0.550252 3.2:-20225294 MS.gene60486:CDS
CTCTCTGACAGTGCTTCTGA+AGG 0.553902 3.2:-20225699 MS.gene60486:CDS
AATGCCGTCCACTTGAGGGT+TGG 0.553905 3.2:+20224147 None:intergenic
CGGACAAGGCTCTGCAAAGA+CGG 0.555061 3.2:-20225237 MS.gene60486:CDS
TAGGATATCAATACTCCCAT+AGG 0.558003 3.2:+20225968 None:intergenic
GACATGACATTTGTGTTGAT+GGG 0.559180 3.2:+20224610 None:intergenic
GAAGCAGCTGATCGGGGTGA+AGG 0.560891 3.2:+20225129 None:intergenic
TTTAAGCCTTCGAGCTTATG+GGG 0.562013 3.2:+20225741 None:intergenic
ATGGTCTCTCTTCTCCTGGA+AGG 0.562685 3.2:-20224546 MS.gene60486:CDS
TGGGATCCTTGACAAGGGAA+TGG 0.563496 3.2:+20224230 None:intergenic
GTTTCCATGGTCAAAAGAGG+TGG 0.566182 3.2:+20224282 None:intergenic
TACTGAGCCTGGTGGAATAG+GGG 0.568600 3.2:+20224568 None:intergenic
CGACCGACTGAAATAGCACC+AGG 0.568814 3.2:+20224799 None:intergenic
GACAGCAGATTTGTGTGTTG+GGG 0.570940 3.2:+20224669 None:intergenic
ATCATCTTCCTCTTCATCTG+CGG 0.571871 3.2:-20226222 MS.gene60486:CDS
AGGCTCTGCAAAGACGGTAT+GGG 0.573441 3.2:-20225231 MS.gene60486:CDS
CGTCCTGGTGCTATTTCAGT+CGG 0.576475 3.2:-20224802 MS.gene60486:CDS
GAGAAATTAACATCAGCCTC+AGG 0.578710 3.2:+20225927 None:intergenic
GTTAACTCCTGGTCTAAGTG+GGG 0.581669 3.2:-20224370 MS.gene60486:CDS
TGCTTCTACTGCTAATGTCA+TGG 0.583456 3.2:-20225112 MS.gene60486:CDS
AAAGAATCCCAACCCTCAAG+TGG 0.585936 3.2:-20224155 MS.gene60486:CDS
TGGGTCTCCATCCTCTCCTG+TGG 0.587335 3.2:-20225595 MS.gene60486:CDS
CCGAGAACTCATTGGAGAGA+GGG 0.587752 3.2:+20224501 None:intergenic
AGCATTAACCGAACCACTTG+AGG 0.589777 3.2:+20225866 None:intergenic
GATAAAGGAGCAGTTGAACC+AGG 0.590443 3.2:+20225630 None:intergenic
GTTGTTCCCCATAAGCTCGA+AGG 0.592106 3.2:-20225747 MS.gene60486:CDS
AGAGCTGGCACATTGGGCTG+TGG 0.592460 3.2:+20224976 None:intergenic
AATGGAGAAGAGCCTGATGT+AGG 0.593112 3.2:-20224259 MS.gene60486:CDS
TGTAGAATTAACAGCTGAGT+CGG 0.595082 3.2:+20225560 None:intergenic
TGAGAGTATGCACGAGAACA+AGG 0.597066 3.2:+20225438 None:intergenic
CGGCAGGGTCAGAATACCGA+GGG 0.597848 3.2:+20224707 None:intergenic
TTAACTCCTGGTCTAAGTGG+GGG 0.601018 3.2:-20224369 MS.gene60486:CDS
AGCTGTAGTGAAACTTTCTA+GGG 0.601845 3.2:+20225361 None:intergenic
CAGGTGTGGGATCCTTGACA+AGG 0.602748 3.2:+20224224 None:intergenic
GACATCAAAGAGATTGATGA+TGG 0.604648 3.2:+20225054 None:intergenic
ACGGTATGGGTTGTAACCGA+AGG 0.606397 3.2:-20225218 MS.gene60486:CDS
AATCCCAACCCTCAAGTGGA+CGG 0.608931 3.2:-20224151 MS.gene60486:CDS
TGGGGATAGAGGCTGTGCAA+AGG 0.609012 3.2:+20225023 None:intergenic
TATTGGAAAAGCTGATTGGT+CGG 0.609121 3.2:-20224338 MS.gene60486:CDS
CGGTATGGGTTGTAACCGAA+GGG 0.616814 3.2:-20225217 MS.gene60486:CDS
AAAATACAGAAGCCGCGGCA+GGG 0.617946 3.2:+20224692 None:intergenic
TCAGTTAGTTGAAGGATGTG+TGG 0.618073 3.2:-20226278 None:intergenic
ACTTAGACCAGGAGTTAACA+GGG 0.618370 3.2:+20224374 None:intergenic
CGACTGAAATAGCACCAGGA+CGG 0.619234 3.2:+20224803 None:intergenic
TAAATGAAGAGCTGGCACAT+TGG 0.622782 3.2:+20224969 None:intergenic
TGAAGGAACAGCAGATCCAG+TGG 0.625826 3.2:+20225146 None:intergenic
GCTGATGTTAATTTCTCCTG+TGG 0.627824 3.2:-20225921 MS.gene60486:CDS
TACTGCTAATGTCATGGACA+TGG 0.629491 3.2:-20225106 MS.gene60486:CDS
GCAGTAGAAGCAGCTGATCG+GGG 0.631967 3.2:+20225123 None:intergenic
ACCGAGAACTCATTGGAGAG+AGG 0.634355 3.2:+20224500 None:intergenic
GCACCGAACTCCTTTAATGG+TGG 0.636101 3.2:-20225997 MS.gene60486:CDS
TCGGTAAATCCAAATGACTT+CGG 0.640900 3.2:-20224319 MS.gene60486:CDS
ATATGTGTGAATATGCTCAT+GGG 0.645475 3.2:-20225293 MS.gene60486:CDS
GTTAGTTGAAGGATGTGTGG+TGG 0.646206 3.2:-20226275 None:intergenic
AGAGCATCCCCTATTCCACC+AGG 0.647565 3.2:-20224575 MS.gene60486:CDS
ATCCTGGAGAGAATGCTCGA+AGG 0.652913 3.2:-20225389 MS.gene60486:CDS
ACTGAAATAGCACCAGGACG+GGG 0.653843 3.2:+20224805 None:intergenic
ACCCAGCTCAGTATCGGACA+AGG 0.655881 3.2:-20225251 MS.gene60486:CDS
GCTTATACTCTCATATCCTG+AGG 0.665811 3.2:-20225943 MS.gene60486:CDS
TGGGGAAAATACAGAAGCCG+CGG 0.666181 3.2:+20224687 None:intergenic
AGATTGATGATGGTGAACCA+GGG 0.666625 3.2:+20225064 None:intergenic
TCGGTATTCTGACCCTGCCG+CGG 0.669451 3.2:-20224704 MS.gene60486:CDS
TTGTTGAAGTCATCATGTAG+CGG 0.680309 3.2:+20224889 None:intergenic
GAACCTTGAATGAATACATG+CGG 0.683334 3.2:+20225463 None:intergenic
AGGTGTGGGATCCTTGACAA+GGG 0.686726 3.2:+20224225 None:intergenic
ATACATGCGGAATTCATCTG+TGG 0.687672 3.2:+20225476 None:intergenic
GCGGCAGGGTCAGAATACCG+AGG 0.701862 3.2:+20224706 None:intergenic
AGAGACCATACTGAGCCTGG+TGG 0.702718 3.2:+20224560 None:intergenic
TGGTGCTGATATCAATTCGG+TGG 0.710852 3.2:-20225814 MS.gene60486:CDS
TATGTGTGAATATGCTCATG+GGG 0.717472 3.2:-20225292 MS.gene60486:CDS
GAACAACAATAACATCGACA+GGG 0.720452 3.2:+20225775 None:intergenic
GATCCAGTGGACACATACAG+CGG 0.722026 3.2:+20225159 None:intergenic
CTTAGACCAGGAGTTAACAG+GGG 0.765437 3.2:+20224375 None:intergenic
GGCAGGGTCAGAATACCGAG+GGG 0.768209 3.2:+20224708 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATAGATCATCAAGATTTGA+TGG + chr3.2:20225602-20225621 None:intergenic 25.0%
! GAAATCTTCAAAATCATTGT+TGG + chr3.2:20224244-20224263 None:intergenic 25.0%
!!! ATAAAATTGCTTTTATCAGC+TGG - chr3.2:20224516-20224535 MS.gene60486:CDS 25.0%
!!! CAATGATTTTGAAGATTTCA+AGG - chr3.2:20224245-20224264 MS.gene60486:CDS 25.0%
AATATGTTGCAAGACTTTGA+TGG - chr3.2:20225482-20225501 MS.gene60486:CDS 30.0%
ATATGTGTGAATATGCTCAT+GGG - chr3.2:20225057-20225076 MS.gene60486:CDS 30.0%
ATATGTTGCAAGACTTTGAT+GGG - chr3.2:20225483-20225502 MS.gene60486:CDS 30.0%
CTTTATCATCTGCTGAAAAT+GGG - chr3.2:20224736-20224755 MS.gene60486:CDS 30.0%
GCTTCACTTATTAATGAAGT+TGG - chr3.2:20224285-20224304 MS.gene60486:CDS 30.0%
TAAGCTGGAAAAAGAATCAT+CGG + chr3.2:20224208-20224227 None:intergenic 30.0%
! GATATGAGAGTATAAGCTTT+AGG + chr3.2:20224404-20224423 None:intergenic 30.0%
! TTATTAATGAAGTTGGCTTC+TGG - chr3.2:20224292-20224311 MS.gene60486:CDS 30.0%
! TTTTTATGTCAGGAAGAGAT+GGG + chr3.2:20224842-20224861 None:intergenic 30.0%
!!! TACATGCTGTTTTTTATGTC+AGG + chr3.2:20224852-20224871 None:intergenic 30.0%
!!! TTTTTTATGTCAGGAAGAGA+TGG + chr3.2:20224843-20224862 None:intergenic 30.0%
AGACATGACATTTGTGTTGA+TGG + chr3.2:20225744-20225763 None:intergenic 35.0%
AGCTGTAGTGAAACTTTCTA+GGG + chr3.2:20224992-20225011 None:intergenic 35.0%
ATTCCGCATGTATTCATTCA+AGG - chr3.2:20224884-20224903 MS.gene60486:CDS 35.0%
ATTGTTTCCATGGTCAAAAG+AGG + chr3.2:20226074-20226093 None:intergenic 35.0%
CCTTTATCATCTGCTGAAAA+TGG - chr3.2:20224735-20224754 MS.gene60486:CDS 35.0%
CTAGTTTGATTAATGCTTCC+TGG + chr3.2:20225407-20225426 None:intergenic 35.0%
GAACAACAATAACATCGACA+GGG + chr3.2:20224578-20224597 None:intergenic 35.0%
GAACCTTGAATGAATACATG+CGG + chr3.2:20224890-20224909 None:intergenic 35.0%
GACATCAAAGAGATTGATGA+TGG + chr3.2:20225299-20225318 None:intergenic 35.0%
GATATGTGTGAATATGCTCA+TGG - chr3.2:20225056-20225075 MS.gene60486:CDS 35.0%
TAGGATATCAATACTCCCAT+AGG + chr3.2:20224385-20224404 None:intergenic 35.0%
TATGTGTGAATATGCTCATG+GGG - chr3.2:20225058-20225077 MS.gene60486:CDS 35.0%
TATTGGAAAAGCTGATTGGT+CGG - chr3.2:20226012-20226031 MS.gene60486:CDS 35.0%
TCGGTAAATCCAAATGACTT+CGG - chr3.2:20226031-20226050 MS.gene60486:CDS 35.0%
TGTAGAATTAACAGCTGAGT+CGG + chr3.2:20224793-20224812 None:intergenic 35.0%
TTGTTGAAGTCATCATGTAG+CGG + chr3.2:20225464-20225483 None:intergenic 35.0%
! ATTATTGCCATTTGCAGATG+GGG + chr3.2:20225348-20225367 None:intergenic 35.0%
! ATTTGTGTTGATGGGTGATA+AGG + chr3.2:20225735-20225754 None:intergenic 35.0%
! CATTATTGCCATTTGCAGAT+GGG + chr3.2:20225349-20225368 None:intergenic 35.0%
! CCATTTTCAGCAGATGATAA+AGG + chr3.2:20224738-20224757 None:intergenic 35.0%
! GACATGACATTTGTGTTGAT+GGG + chr3.2:20225743-20225762 None:intergenic 35.0%
! TCTTTTGACCATGGAAACAA+TGG - chr3.2:20226073-20226092 MS.gene60486:CDS 35.0%
! TTTGGTTAGATCCCTTTTGA+CGG + chr3.2:20224323-20224342 None:intergenic 35.0%
! TTTTAAGCCTTCGAGCTTAT+GGG + chr3.2:20224613-20224632 None:intergenic 35.0%
!! ATGCCCTGATTTTAGAAAAG+GGG - chr3.2:20225019-20225038 MS.gene60486:CDS 35.0%
!! CATGCCCTGATTTTAGAAAA+GGG - chr3.2:20225018-20225037 MS.gene60486:CDS 35.0%
!! TTTTTAAGCCTTCGAGCTTA+TGG + chr3.2:20224614-20224633 None:intergenic 35.0%
!!! AAGCCCCTTTTCTAAAATCA+GGG + chr3.2:20225025-20225044 None:intergenic 35.0%
!!! ATGGAGTTAGTGTTTTAGAG+CGG + chr3.2:20225583-20225602 None:intergenic 35.0%
AAATGAAGAGCTGGCACATT+GGG + chr3.2:20225383-20225402 None:intergenic 40.0%
AACCAGGACTCGATGAAATA+AGG + chr3.2:20224707-20224726 None:intergenic 40.0%
AAGACAGCAGATTTGTGTGT+TGG + chr3.2:20225686-20225705 None:intergenic 40.0%
ACCAGGACTCGATGAAATAA+GGG + chr3.2:20224706-20224725 None:intergenic 40.0%
ACTTAGACCAGGAGTTAACA+GGG + chr3.2:20225979-20225998 None:intergenic 40.0%
AGACAGCAGATTTGTGTGTT+GGG + chr3.2:20225685-20225704 None:intergenic 40.0%
AGCTTATGGGGAACAACAAT+AGG + chr3.2:20224600-20224619 None:intergenic 40.0%
ATACATGCGGAATTCATCTG+TGG + chr3.2:20224877-20224896 None:intergenic 40.0%
ATCATCTTCCTCTTCATCTG+CGG - chr3.2:20224128-20224147 MS.gene60486:CDS 40.0%
CAGCTGTAGTGAAACTTTCT+AGG + chr3.2:20224993-20225012 None:intergenic 40.0%
CTCCTATTGGAAAAGCTGAT+TGG - chr3.2:20226008-20226027 MS.gene60486:CDS 40.0%
CTCGATGAAATAAGGGACAT+TGG + chr3.2:20224699-20224718 None:intergenic 40.0%
CTGCAAATGGCAATAATGCT+TGG - chr3.2:20225351-20225370 MS.gene60486:CDS 40.0%
GAGAAATTAACATCAGCCTC+AGG + chr3.2:20224426-20224445 None:intergenic 40.0%
GCTGATGTTAATTTCTCCTG+TGG - chr3.2:20224429-20224448 MS.gene60486:CDS 40.0%
GCTTATACTCTCATATCCTG+AGG - chr3.2:20224407-20224426 MS.gene60486:CDS 40.0%
GGAACAACAATAACATCGAC+AGG + chr3.2:20224579-20224598 None:intergenic 40.0%
TAAATGAAGAGCTGGCACAT+TGG + chr3.2:20225384-20225403 None:intergenic 40.0%
TACTGCTAATGTCATGGACA+TGG - chr3.2:20225244-20225263 MS.gene60486:CDS 40.0%
TGCTTCTACTGCTAATGTCA+TGG - chr3.2:20225238-20225257 MS.gene60486:CDS 40.0%
TGTGTTTGACCGAAGTCATT+TGG + chr3.2:20226043-20226062 None:intergenic 40.0%
TGTTAACTCCTGGTCTAAGT+GGG - chr3.2:20225979-20225998 MS.gene60486:CDS 40.0%
TTCTCTTTCTTCTCAGGTGT+GGG + chr3.2:20226142-20226161 None:intergenic 40.0%
TTCTGGTATGTCCGTCAAAA+GGG - chr3.2:20224309-20224328 MS.gene60486:CDS 40.0%
TTTAAGCCTTCGAGCTTATG+GGG + chr3.2:20224612-20224631 None:intergenic 40.0%
! ACAGCTGAGTCGGTAAATTT+TGG + chr3.2:20224783-20224802 None:intergenic 40.0%
! AGTGCTTTTATCAGTTCCAC+AGG + chr3.2:20224448-20224467 None:intergenic 40.0%
! ATCAATTCGGTGGATGCTAA+TGG - chr3.2:20224546-20224565 MS.gene60486:CDS 40.0%
! GACCAATCAGCTTTTCCAAT+AGG + chr3.2:20226013-20226032 None:intergenic 40.0%
! GCATTATTGCCATTTGCAGA+TGG + chr3.2:20225350-20225369 None:intergenic 40.0%
! TCAATTCGGTGGATGCTAAT+GGG - chr3.2:20224547-20224566 MS.gene60486:CDS 40.0%
! TCTTTTCAGCGGATGGAATT+GGG + chr3.2:20226180-20226199 None:intergenic 40.0%
! TTCTTTTCAGCGGATGGAAT+TGG + chr3.2:20226181-20226200 None:intergenic 40.0%
!! AGATTGATGATGGTGAACCA+GGG + chr3.2:20225289-20225308 None:intergenic 40.0%
!! AGCTGGTGCTGATATCAATT+CGG - chr3.2:20224533-20224552 MS.gene60486:CDS 40.0%
!! AGCTTTGAAGCTGTTGAAAC+TGG + chr3.2:20225712-20225731 None:intergenic 40.0%
!! CCATGCCCTGATTTTAGAAA+AGG - chr3.2:20225017-20225036 MS.gene60486:CDS 40.0%
!! TTCGGTGCTCAAGAACAATT+TGG + chr3.2:20224341-20224360 None:intergenic 40.0%
!!! CAAGCCCCTTTTCTAAAATC+AGG + chr3.2:20225026-20225045 None:intergenic 40.0%
!!! CCTTTTCTAAAATCAGGGCA+TGG + chr3.2:20225020-20225039 None:intergenic 40.0%
!!! GAGAGTGATTTTGATTCGTC+AGG + chr3.2:20224102-20224121 None:intergenic 40.0%
!!! GGCATTGATCACTCTGTTTT+GGG - chr3.2:20226220-20226239 MS.gene60486:CDS 40.0%
AAAGAATCCCAACCCTCAAG+TGG - chr3.2:20226195-20226214 MS.gene60486:CDS 45.0%
AAAGAGAAGCTTGCAGGTTC+CGG - chr3.2:20226154-20226173 MS.gene60486:CDS 45.0%
AACAATCCTGTCTCAGCTGT+TGG - chr3.2:20225950-20225969 MS.gene60486:CDS 45.0%
AAGGATGTCGCCTAGAAGTA+TGG - chr3.2:20225823-20225842 MS.gene60486:CDS 45.0%
AATGGAGAAGAGCCTGATGT+AGG - chr3.2:20226091-20226110 MS.gene60486:CDS 45.0%
ACAATCCTGTCTCAGCTGTT+GGG - chr3.2:20225951-20225970 MS.gene60486:CDS 45.0%
AGAAGAGCCTGATGTAGGTT+GGG - chr3.2:20226096-20226115 MS.gene60486:CDS 45.0%
AGCATTAACCGAACCACTTG+AGG + chr3.2:20224487-20224506 None:intergenic 45.0%
AGCCTTAGCTCAAGAGAACT+TGG - chr3.2:20225923-20225942 MS.gene60486:CDS 45.0%
ATACTGAGCCTGGTGGAATA+GGG + chr3.2:20225786-20225805 None:intergenic 45.0%
ATCATGTAGCGGCATATCAC+GGG + chr3.2:20225453-20225472 None:intergenic 45.0%
CACCAAGTTCTCTTGAGCTA+AGG + chr3.2:20225928-20225947 None:intergenic 45.0%
CACTTAGACCAGGAGTTAAC+AGG + chr3.2:20225980-20225999 None:intergenic 45.0%
CAGCCACCATTAAAGGAGTT+CGG + chr3.2:20224359-20224378 None:intergenic 45.0%
CATCATGTAGCGGCATATCA+CGG + chr3.2:20225454-20225473 None:intergenic 45.0%
CTCGTGCATACTCTCATGAT+TGG - chr3.2:20224919-20224938 MS.gene60486:CDS 45.0%
CTGAGATTTCTTCATCCCCT+CGG - chr3.2:20225627-20225646 MS.gene60486:CDS 45.0%
CTGTTAACTCCTGGTCTAAG+TGG - chr3.2:20225978-20225997 MS.gene60486:CDS 45.0%
CTTAGACCAGGAGTTAACAG+GGG + chr3.2:20225978-20225997 None:intergenic 45.0%
CTTCCTGGTAAATGAAGAGC+TGG + chr3.2:20225392-20225411 None:intergenic 45.0%
CTTCTCTTTCTTCTCAGGTG+TGG + chr3.2:20226143-20226162 None:intergenic 45.0%
CTTCTGGTATGTCCGTCAAA+AGG - chr3.2:20224308-20224327 MS.gene60486:CDS 45.0%
GACAGCAGATTTGTGTGTTG+GGG + chr3.2:20225684-20225703 None:intergenic 45.0%
GAGAAGAAAGAGAAGCTTGC+AGG - chr3.2:20226148-20226167 MS.gene60486:CDS 45.0%
GATAAAGGAGCAGTTGAACC+AGG + chr3.2:20224723-20224742 None:intergenic 45.0%
GCAAGCTTCTCTTTCTTCTC+AGG + chr3.2:20226148-20226167 None:intergenic 45.0%
GCTCTTCTCCATTGTTTCCA+TGG + chr3.2:20226084-20226103 None:intergenic 45.0%
GTTAACTCCTGGTCTAAGTG+GGG - chr3.2:20225980-20225999 MS.gene60486:CDS 45.0%
GTTTCCATGGTCAAAAGAGG+TGG + chr3.2:20226071-20226090 None:intergenic 45.0%
TAATGGTGGCTGCTTCCTAT+GGG - chr3.2:20224367-20224386 MS.gene60486:CDS 45.0%
TAGCAGTAGAAGCAGCTGAT+CGG + chr3.2:20225232-20225251 None:intergenic 45.0%
TAGGAAGCAGCCACCATTAA+AGG + chr3.2:20224366-20224385 None:intergenic 45.0%
TTAACTCCTGGTCTAAGTGG+GGG - chr3.2:20225981-20226000 MS.gene60486:CDS 45.0%
TTAATGGTGGCTGCTTCCTA+TGG - chr3.2:20224366-20224385 MS.gene60486:CDS 45.0%
TTCTCTCCAGGATGCACAAA+AGG + chr3.2:20224954-20224973 None:intergenic 45.0%
! AGATCCACCTCTTTTGACCA+TGG - chr3.2:20226064-20226083 MS.gene60486:CDS 45.0%
! AGGGTTGGGATTCTTTTCAG+CGG + chr3.2:20226191-20226210 None:intergenic 45.0%
! ATGATGGTGAACCAGGGAAA+AGG + chr3.2:20225283-20225302 None:intergenic 45.0%
! TCCCTTATTTCATCGAGTCC+TGG - chr3.2:20224702-20224721 MS.gene60486:CDS 45.0%
! TGAGAGTATGCACGAGAACA+AGG + chr3.2:20224915-20224934 None:intergenic 45.0%
! TTGGGATTCTTTTCAGCGGA+TGG + chr3.2:20226187-20226206 None:intergenic 45.0%
!! GAGATTGATGATGGTGAACC+AGG + chr3.2:20225290-20225309 None:intergenic 45.0%
!! TGAGCACCGAACTCCTTTAA+TGG - chr3.2:20224350-20224369 MS.gene60486:CDS 45.0%
!! TGGTGCTGATATCAATTCGG+TGG - chr3.2:20224536-20224555 MS.gene60486:CDS 45.0%
!!! AAATTTTGGAGCCACAGGAG+AGG + chr3.2:20224769-20224788 None:intergenic 45.0%
!!! ATCACTCTGTTTTGGGAGCT+TGG - chr3.2:20226227-20226246 MS.gene60486:CDS 45.0%
!!! CGGCATTGATCACTCTGTTT+TGG - chr3.2:20226219-20226238 MS.gene60486:CDS 45.0%
!!! TCGGTAAATTTTGGAGCCAC+AGG + chr3.2:20224774-20224793 None:intergenic 45.0%
AAAAGGGGCTTGCAGACGTT+CGG - chr3.2:20225034-20225053 MS.gene60486:CDS 50.0%
AAAATACAGAAGCCGCGGCA+GGG + chr3.2:20225661-20225680 None:intergenic 50.0%
AAGAGAGACCATACTGAGCC+TGG + chr3.2:20225796-20225815 None:intergenic 50.0%
AAGGCTCTGCAAAGACGGTA+TGG - chr3.2:20225118-20225137 MS.gene60486:CDS 50.0%
AAGTGGGGGTCATCTCCTAT+TGG - chr3.2:20225995-20226014 MS.gene60486:CDS 50.0%
AATCCCAACCCTCAAGTGGA+CGG - chr3.2:20226199-20226218 MS.gene60486:CDS 50.0%
ACCGAGAACTCATTGGAGAG+AGG + chr3.2:20225853-20225872 None:intergenic 50.0%
ACTGAAATAGCACCAGGACG+GGG + chr3.2:20225548-20225567 None:intergenic 50.0%
AGCAGTAGAAGCAGCTGATC+GGG + chr3.2:20225231-20225250 None:intergenic 50.0%
AGGCTCTGCAAAGACGGTAT+GGG - chr3.2:20225119-20225138 MS.gene60486:CDS 50.0%
AGGGGATGCTCTACGTTTGT+AGG + chr3.2:20225767-20225786 None:intergenic 50.0%
AGGTGTGGGATCCTTGACAA+GGG + chr3.2:20226128-20226147 None:intergenic 50.0%
ATCCTGGAGAGAATGCTCGA+AGG - chr3.2:20224961-20224980 MS.gene60486:CDS 50.0%
ATGGTCTCTCTTCTCCTGGA+AGG - chr3.2:20225804-20225823 MS.gene60486:CDS 50.0%
CAGTATGGTCTCTCTTCTCC+TGG - chr3.2:20225800-20225819 MS.gene60486:CDS 50.0%
CATACTGAGCCTGGTGGAAT+AGG + chr3.2:20225787-20225806 None:intergenic 50.0%
CCATTTGCAGATGGGGATAG+AGG + chr3.2:20225341-20225360 None:intergenic 50.0%
CCCTCTCTCCAATGAGTTCT+CGG - chr3.2:20225849-20225868 MS.gene60486:CDS 50.0%
CCGAGAACTCATTGGAGAGA+GGG + chr3.2:20225852-20225871 None:intergenic 50.0%
CCTCTATCCCCATCTGCAAA+TGG - chr3.2:20225338-20225357 MS.gene60486:CDS 50.0%
CGACTGAAATAGCACCAGGA+CGG + chr3.2:20225550-20225569 None:intergenic 50.0%
CTATTCCACCAGGCTCAGTA+TGG - chr3.2:20225785-20225804 MS.gene60486:CDS 50.0%
CTCTCTGACAGTGCTTCTGA+AGG - chr3.2:20224651-20224670 MS.gene60486:CDS 50.0%
GAATGGACCCAACCTACATC+AGG + chr3.2:20226106-20226125 None:intergenic 50.0%
GACTGAAATAGCACCAGGAC+GGG + chr3.2:20225549-20225568 None:intergenic 50.0%
GAGAAGAGCCTGATGTAGGT+TGG - chr3.2:20226095-20226114 MS.gene60486:CDS 50.0%
GAGAGGGCTTCCATACTTCT+AGG + chr3.2:20225836-20225855 None:intergenic 50.0%
GATCAATGCCGTCCACTTGA+GGG + chr3.2:20226210-20226229 None:intergenic 50.0%
GATCCAGTGGACACATACAG+CGG + chr3.2:20225194-20225213 None:intergenic 50.0%
GCACCGAACTCCTTTAATGG+TGG - chr3.2:20224353-20224372 MS.gene60486:CDS 50.0%
GCTGACAACCGAGAACTCAT+TGG + chr3.2:20225860-20225879 None:intergenic 50.0%
GTGCCAGCTCTTCATTTACC+AGG - chr3.2:20225386-20225405 MS.gene60486:CDS 50.0%
GTTGTTCCCCATAAGCTCGA+AGG - chr3.2:20224603-20224622 MS.gene60486:CDS 50.0%
TACTGAGCCTGGTGGAATAG+GGG + chr3.2:20225785-20225804 None:intergenic 50.0%
TCAGGAAGAGATGGGTCAAC+TGG + chr3.2:20224834-20224853 None:intergenic 50.0%
TGAAGGAACAGCAGATCCAG+TGG + chr3.2:20225207-20225226 None:intergenic 50.0%
TGATCAATGCCGTCCACTTG+AGG + chr3.2:20226211-20226230 None:intergenic 50.0%
TGGGATCCTTGACAAGGGAA+TGG + chr3.2:20226123-20226142 None:intergenic 50.0%
TGGGGAAAATACAGAAGCCG+CGG + chr3.2:20225666-20225685 None:intergenic 50.0%
TTGGGTCCATTCCCTTGTCA+AGG - chr3.2:20226114-20226133 MS.gene60486:CDS 50.0%
! ACGGTATGGGTTGTAACCGA+AGG - chr3.2:20225132-20225151 MS.gene60486:CDS 50.0%
! CGGTATGGGTTGTAACCGAA+GGG - chr3.2:20225133-20225152 MS.gene60486:CDS 50.0%
! CTCATGGGGTATTTGAGTGC+TGG - chr3.2:20225072-20225091 MS.gene60486:CDS 50.0%
! GAGCGAAAAAGCACACCCTT+CGG + chr3.2:20225151-20225170 None:intergenic 50.0%
! GAGTGTCCTTTTGTGCATCC+TGG - chr3.2:20224945-20224964 MS.gene60486:CDS 50.0%
! GCTATTTCAGTCGGTCGATC+TGG - chr3.2:20225557-20225576 MS.gene60486:CDS 50.0%
! GGAAGCATGTTCGAGTAAGC+TGG + chr3.2:20224223-20224242 None:intergenic 50.0%
!! CGTCCTGGTGCTATTTCAGT+CGG - chr3.2:20225548-20225567 MS.gene60486:CDS 50.0%
AATGCCGTCCACTTGAGGGT+TGG + chr3.2:20226206-20226225 None:intergenic 55.0%
ACCTTCAGCCGCAGATGAAG+AGG + chr3.2:20224139-20224158 None:intergenic 55.0%
AGAGACCATACTGAGCCTGG+TGG + chr3.2:20225793-20225812 None:intergenic 55.0%
AGATGACCCCCACTTAGACC+AGG + chr3.2:20225990-20226009 None:intergenic 55.0%
ATGCCGTCCACTTGAGGGTT+GGG + chr3.2:20226205-20226224 None:intergenic 55.0%
CAGGTGTGGGATCCTTGACA+AGG + chr3.2:20226129-20226148 None:intergenic 55.0%
CGACCGACTGAAATAGCACC+AGG + chr3.2:20225554-20225573 None:intergenic 55.0%
CGGACAAGGCTCTGCAAAGA+CGG - chr3.2:20225113-20225132 MS.gene60486:CDS 55.0%
CTCCTTCGAGCATTCTCTCC+AGG + chr3.2:20224966-20224985 None:intergenic 55.0%
CTGTGCTGCCTCAAGTGGTT+CGG - chr3.2:20224476-20224495 MS.gene60486:CDS 55.0%
CTTCACTGTGCTGCCTCAAG+TGG - chr3.2:20224471-20224490 MS.gene60486:CDS 55.0%
CTTCTAGGCGACATCCTTCC+AGG + chr3.2:20225821-20225840 None:intergenic 55.0%
GAAAATACAGAAGCCGCGGC+AGG + chr3.2:20225662-20225681 None:intergenic 55.0%
GCAGTAGAAGCAGCTGATCG+GGG + chr3.2:20225230-20225249 None:intergenic 55.0%
GCCTTGTCCGATACTGAGCT+GGG + chr3.2:20225103-20225122 None:intergenic 55.0%
GGCTACACCCAGCTCAGTAT+CGG - chr3.2:20225093-20225112 MS.gene60486:CDS 55.0%
GGGGATAGAGGCTGTGCAAA+GGG + chr3.2:20225329-20225348 None:intergenic 55.0%
TCCTCTTCATCTGCGGCTGA+AGG - chr3.2:20224135-20224154 MS.gene60486:CDS 55.0%
TGGGGATAGAGGCTGTGCAA+AGG + chr3.2:20225330-20225349 None:intergenic 55.0%
TTCAGCGGATGGAATTGGGC+CGG + chr3.2:20226176-20226195 None:intergenic 55.0%
TTGGGTCCCCTGTTAACTCC+TGG - chr3.2:20225969-20225988 MS.gene60486:CDS 55.0%
! ACCCAGCTCAGTATCGGACA+AGG - chr3.2:20225099-20225118 MS.gene60486:CDS 55.0%
! AGAGCATCCCCTATTCCACC+AGG - chr3.2:20225775-20225794 MS.gene60486:CDS 55.0%
! AGCCTTGTCCGATACTGAGC+TGG + chr3.2:20225104-20225123 None:intergenic 55.0%
! GCTGCTATGAGCCTTTTCCC+TGG - chr3.2:20225269-20225288 MS.gene60486:CDS 55.0%
! TTGGCTTGAGCAACTGCAGC+TGG - chr3.2:20226246-20226265 MS.gene60486:CDS 55.0%
! TTTGGAGCCACAGGAGAGGA+TGG + chr3.2:20224765-20224784 None:intergenic 55.0%
AGAGCTGGCACATTGGGCTG+TGG + chr3.2:20225377-20225396 None:intergenic 60.0%
CGGCAGGGTCAGAATACCGA+GGG + chr3.2:20225646-20225665 None:intergenic 60.0%
CGTCCGCTGTATGTGTCCAC+TGG - chr3.2:20225188-20225207 MS.gene60486:CDS 60.0%
GAAGCAGCTGATCGGGGTGA+AGG + chr3.2:20225224-20225243 None:intergenic 60.0%
GGCAGGGTCAGAATACCGAG+GGG + chr3.2:20225645-20225664 None:intergenic 60.0%
TACAGCGGACGCAGCTCTTC+AGG + chr3.2:20225179-20225198 None:intergenic 60.0%
TCGGTATTCTGACCCTGCCG+CGG - chr3.2:20225646-20225665 MS.gene60486:CDS 60.0%
TGGGTCTCCATCCTCTCCTG+TGG - chr3.2:20224755-20224774 MS.gene60486:CDS 60.0%
TGTTTCTCACAGCCCCGTCC+TGG - chr3.2:20225533-20225552 MS.gene60486:CDS 60.0%
GCGGCAGGGTCAGAATACCG+AGG + chr3.2:20225647-20225666 None:intergenic 65.0%
GGGGACCCAACAGCTGAGAC+AGG + chr3.2:20225959-20225978 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 20224087 20226285 20224087 ID=MS.gene60486
chr3.2 mRNA 20224087 20226285 20224087 ID=MS.gene60486.t1;Parent=MS.gene60486
chr3.2 exon 20224087 20226285 20224087 ID=MS.gene60486.t1.exon1;Parent=MS.gene60486.t1
chr3.2 CDS 20224087 20226285 20224087 ID=cds.MS.gene60486.t1;Parent=MS.gene60486.t1
Gene Sequence

>MS.gene60486

ATGTGTGGTGGACCTGACGAATCAAAATCACTCTCAACTTCATCATCTTCCTCTTCATCTGCGGCTGAAGGTTCAACTTCAAATACTGTCAAAGACATGAAAAATCTAACTGTTCGTACCGATGATTCTTTTTCCAGCTTACTCGAACATGCTTCCAACAATGATTTTGAAGATTTCAAGGTAGCTCTAGATAGTGATGCTTCACTTATTAATGAAGTTGGCTTCTGGTATGTCCGTCAAAAGGGATCTAACCAAATTGTTCTTGAGCACCGAACTCCTTTAATGGTGGCTGCTTCCTATGGGAGTATTGATATCCTAAAGCTTATACTCTCATATCCTGAGGCTGATGTTAATTTCTCCTGTGGAACTGATAAAAGCACTGCTCTTCACTGTGCTGCCTCAAGTGGTTCGGTTAATGCTGTTGACGCTATAAAATTGCTTTTATCAGCTGGTGCTGATATCAATTCGGTGGATGCTAATGGGAAACGCCCTGTCGATGTTATTGTTGTTCCTATTGTTGTTCCCCATAAGCTCGAAGGCTTAAAAACAATTCTTGAAGAACTTCTCTCTGACAGTGCTTCTGAAGGATCTGTTGATGATTGCTCTCTTCCAATGTCCCTTATTTCATCGAGTCCTGGTTCAACTGCTCCTTTATCATCTGCTGAAAATGGGTCTCCATCCTCTCCTGTGGCTCCAAAATTTACCGACTCAGCTGTTAATTCTACATCAGAGAAGAAAGAGTATCCAGTTGACCCATCTCTTCCTGACATAAAAAACAGCATGTATGCCACAGATGAATTCCGCATGTATTCATTCAAGGTTCGTCCTTGTTCTCGTGCATACTCTCATGATTGGACAGAGTGTCCTTTTGTGCATCCTGGAGAGAATGCTCGAAGGAGAGACCCTAGAAAGTTTCACTACAGCTGTGTGCCATGCCCTGATTTTAGAAAAGGGGCTTGCAGACGTTCGGATATGTGTGAATATGCTCATGGGGTATTTGAGTGCTGGCTACACCCAGCTCAGTATCGGACAAGGCTCTGCAAAGACGGTATGGGTTGTAACCGAAGGGTGTGCTTTTTCGCTCACTCACCTGAAGAGCTGCGTCCGCTGTATGTGTCCACTGGATCTGCTGTTCCTTCACCCCGATCAGCTGCTTCTACTGCTAATGTCATGGACATGGCTGCTGCTATGAGCCTTTTCCCTGGTTCACCATCATCAATCTCTTTGATGTCTCAATCACCCTTTGCACAGCCTCTATCCCCATCTGCAAATGGCAATAATGCTTGGCCACAGCCCAATGTGCCAGCTCTTCATTTACCAGGAAGCATTAATCAAACTAGTCGTTTGAGATCTTCTCTCAGTGCCCGTGATATGCCGCTACATGATGACTTCAACAATATGTTGCAAGACTTTGATGGGCAGCAGCAGATTCTGAATGACTTGAGCTGTTTCTCACAGCCCCGTCCTGGTGCTATTTCAGTCGGTCGATCTGGCCGCTCTAAAACACTAACTCCATCAAATCTTGATGATCTATTTTGTGCTGAGATTTCTTCATCCCCTCGGTATTCTGACCCTGCCGCGGCTTCTGTATTTTCCCCAACACACAAATCTGCTGTCTTCAACCAGTTTCAACAGCTTCAAAGCTCCTTATCACCCATCAACACAAATGTCATGTCTCCTACAAACGTAGAGCATCCCCTATTCCACCAGGCTCAGTATGGTCTCTCTTCTCCTGGAAGGATGTCGCCTAGAAGTATGGAAGCCCTCTCTCCAATGAGTTCTCGGTTGTCAGCTTTTGCTCAGCGTGAGAAACAGCAGCAGCAGCAACAGCTGCGTAGCCTTAGCTCAAGAGAACTTGGTGCTAACAATCCTGTCTCAGCTGTTGGGTCCCCTGTTAACTCCTGGTCTAAGTGGGGGTCATCTCCTATTGGAAAAGCTGATTGGTCGGTAAATCCAAATGACTTCGGTCAAACACAGAGATCCACCTCTTTTGACCATGGAAACAATGGAGAAGAGCCTGATGTAGGTTGGGTCCATTCCCTTGTCAAGGATCCCACACCTGAGAAGAAAGAGAAGCTTGCAGGTTCCGGCCCAATTCCATCCGCTGAAAAGAATCCCAACCCTCAAGTGGACGGCATTGATCACTCTGTTTTGGGAGCTTGGCTTGAGCAACTGCAGCTGGATCAACTTGTAGTCTAG
Protein sequence

>MS.gene60486.t1

MCGGPDESKSLSTSSSSSSSAAEGSTSNTVKDMKNLTVRTDDSFSSLLEHASNNDFEDFKVALDSDASLINEVGFWYVRQKGSNQIVLEHRTPLMVAASYGSIDILKLILSYPEADVNFSCGTDKSTALHCAASSGSVNAVDAIKLLLSAGADINSVDANGKRPVDVIVVPIVVPHKLEGLKTILEELLSDSASEGSVDDCSLPMSLISSSPGSTAPLSSAENGSPSSPVAPKFTDSAVNSTSEKKEYPVDPSLPDIKNSMYATDEFRMYSFKVRPCSRAYSHDWTECPFVHPGENARRRDPRKFHYSCVPCPDFRKGACRRSDMCEYAHGVFECWLHPAQYRTRLCKDGMGCNRRVCFFAHSPEELRPLYVSTGSAVPSPRSAASTANVMDMAAAMSLFPGSPSSISLMSQSPFAQPLSPSANGNNAWPQPNVPALHLPGSINQTSRLRSSLSARDMPLHDDFNNMLQDFDGQQQILNDLSCFSQPRPGAISVGRSGRSKTLTPSNLDDLFCAEISSSPRYSDPAAASVFSPTHKSAVFNQFQQLQSSLSPINTNVMSPTNVEHPLFHQAQYGLSSPGRMSPRSMEALSPMSSRLSAFAQREKQQQQQQLRSLSSRELGANNPVSAVGSPVNSWSKWGSSPIGKADWSVNPNDFGQTQRSTSFDHGNNGEEPDVGWVHSLVKDPTPEKKEKLAGSGPIPSAEKNPNPQVDGIDHSVLGAWLEQLQLDQLVV