AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60590.t1 MTR_1g093080 96.639 357 12 0 1 357 1 357 0.0 719
MS.gene60590.t1 MTR_1g093080 96.552 261 9 0 1 261 1 261 0.0 526
MS.gene60590.t1 MTR_1g093080 96.186 236 9 0 1 236 1 236 2.08e-170 476
MS.gene60590.t1 MTR_5g054300 58.268 381 121 15 1 374 10 359 1.08e-125 366
MS.gene60590.t1 MTR_4g113140 35.093 322 145 7 2 294 29 315 9.48e-48 168
MS.gene60590.t1 MTR_4g086835 34.648 355 147 11 10 309 44 368 5.93e-42 152
MS.gene60590.t1 MTR_4g086835 34.648 355 146 12 10 309 44 367 3.77e-40 147
MS.gene60590.t1 MTR_5g017980 31.343 335 143 12 2 324 35 294 1.85e-32 124
MS.gene60590.t1 MTR_5g017980 31.940 335 139 13 2 324 35 292 6.46e-32 123
MS.gene60590.t1 MTR_5g017980 31.642 335 141 13 2 324 35 293 9.00e-31 120
MS.gene60590.t1 MTR_2g027860 57.576 66 28 0 211 276 49 114 1.35e-16 80.5
MS.gene60590.t1 MTR_7g088070 55.556 72 30 1 211 280 50 121 9.42e-16 77.0
MS.gene60590.t1 MTR_7g088070 56.923 65 28 0 211 275 50 114 1.19e-15 77.0
MS.gene60590.t1 MTR_2g034960 37.008 127 57 4 210 323 210 326 5.14e-15 77.0
MS.gene60590.t1 MTR_7g115530 43.617 94 51 1 211 304 218 309 9.36e-15 75.1
MS.gene60590.t1 MTR_4g121020 53.521 71 32 1 210 280 110 179 1.71e-14 75.5
MS.gene60590.t1 MTR_4g121020 53.521 71 32 1 210 280 214 283 1.85e-14 75.5
MS.gene60590.t1 MTR_4g121020 53.521 71 32 1 210 280 214 283 1.92e-14 75.1
MS.gene60590.t1 MTR_2g450070 37.607 117 64 4 209 323 211 320 2.97e-14 74.7
MS.gene60590.t1 MTR_2g450070 37.607 117 64 4 209 323 211 320 2.97e-14 74.7
MS.gene60590.t1 MTR_4g131580 53.968 63 28 1 209 271 198 259 4.13e-14 74.3
MS.gene60590.t1 MTR_2g027800 61.818 55 21 0 211 265 39 93 4.68e-14 72.4
MS.gene60590.t1 MTR_1g032570 48.611 72 36 1 200 271 120 190 5.42e-14 73.6
MS.gene60590.t1 MTR_2g027800 61.818 55 21 0 211 265 39 93 7.14e-14 72.0
MS.gene60590.t1 MTR_1g032570 48.611 72 36 1 200 271 189 259 7.37e-14 73.6
MS.gene60590.t1 MTR_3g102600 43.038 79 43 2 215 292 87 164 1.05e-13 69.3
MS.gene60590.t1 MTR_3g106220 45.679 81 42 2 204 284 191 269 1.11e-13 72.8
MS.gene60590.t1 MTR_6g444980 61.818 55 21 0 211 265 35 89 1.39e-13 71.6
MS.gene60590.t1 MTR_7g117705 48.611 72 36 1 209 280 189 259 1.39e-13 72.4
MS.gene60590.t1 MTR_5g014040 51.515 66 31 1 204 269 304 368 1.64e-13 72.4
MS.gene60590.t1 MTR_6g444980 61.818 55 21 0 211 265 35 89 1.69e-13 71.2
MS.gene60590.t1 MTR_3g102600 43.038 79 43 2 215 292 136 213 2.31e-13 69.3
MS.gene60590.t1 MTR_4g131570 50.000 66 32 1 206 271 198 262 2.34e-13 72.0
MS.gene60590.t1 MTR_7g115530 54.839 62 28 0 211 272 218 279 2.43e-13 70.5
MS.gene60590.t1 MTR_1g090670 60.000 55 22 0 211 265 44 98 3.70e-13 69.7
MS.gene60590.t1 MTR_7g115530 54.839 62 28 0 211 272 218 279 4.54e-13 69.7
MS.gene60590.t1 MTR_5g041350 60.000 55 22 0 211 265 15 69 5.91e-13 69.7
MS.gene60590.t1 MTR_1g013170 52.381 63 29 1 209 271 189 250 7.08e-13 70.1
MS.gene60590.t1 MTR_7g115530 54.839 62 28 0 211 272 218 279 7.90e-13 69.7
MS.gene60590.t1 MTR_7g098250 43.902 82 44 1 211 292 262 341 8.20e-13 70.1
MS.gene60590.t1 MTR_5g027440 52.459 61 27 1 211 269 25 85 8.74e-13 68.6
MS.gene60590.t1 MTR_1g080330 50.794 63 31 0 211 273 175 237 1.57e-12 67.8
MS.gene60590.t1 MTR_4g064730 43.243 74 41 1 207 280 127 199 2.09e-12 67.8
MS.gene60590.t1 MTR_3g102590 43.590 78 41 2 209 284 141 217 2.26e-12 66.6
MS.gene60590.t1 MTR_1g080330 50.794 63 31 0 211 273 175 237 2.51e-12 68.2
MS.gene60590.t1 MTR_4g098870 40.426 94 52 2 178 268 274 366 2.53e-12 68.6
MS.gene60590.t1 MTR_4g098870 40.426 94 52 2 178 268 294 386 2.84e-12 68.6
MS.gene60590.t1 MTR_4g098870 40.426 94 52 2 178 268 294 386 2.88e-12 68.6
MS.gene60590.t1 MTR_4g098870 40.426 94 52 2 178 268 274 366 2.89e-12 68.6
MS.gene60590.t1 MTR_4g098870 40.426 94 52 2 178 268 313 405 3.03e-12 68.6
MS.gene60590.t1 MTR_7g089010 40.244 82 45 2 188 269 71 148 3.76e-12 67.0
MS.gene60590.t1 MTR_4g021790 48.571 70 35 1 200 269 157 225 5.76e-12 65.9
MS.gene60590.t1 MTR_6g032990 47.059 68 36 0 211 278 42 109 5.98e-12 67.0
MS.gene60590.t1 MTR_4g081710 56.364 55 24 0 211 265 17 71 6.12e-12 66.6
MS.gene60590.t1 MTR_2g086450 54.545 55 25 0 211 265 45 99 7.09e-12 66.6
MS.gene60590.t1 MTR_4g131600 32.143 140 81 5 204 342 193 319 7.78e-12 67.0
MS.gene60590.t1 MTR_4g078810 37.895 95 46 2 209 303 156 237 8.54e-12 66.6
MS.gene60590.t1 MTR_8g079940 46.479 71 37 1 210 280 208 277 8.59e-12 67.0
MS.gene60590.t1 MTR_1g053835 50.000 60 30 0 211 270 258 317 8.85e-12 66.6
MS.gene60590.t1 MTR_1g053830 38.462 78 33 1 188 265 10 72 8.96e-12 61.6
MS.gene60590.t1 MTR_5g014040 47.887 71 31 2 204 269 264 333 1.30e-11 66.2
MS.gene60590.t1 MTR_2g023580 55.769 52 23 0 213 264 57 108 1.49e-11 64.3
MS.gene60590.t1 MTR_2g090675 45.070 71 38 1 209 279 159 228 1.49e-11 65.9
MS.gene60590.t1 MTR_5g014040 47.887 71 31 2 204 269 304 373 1.56e-11 66.2
MS.gene60590.t1 MTR_3g086100 40.698 86 45 2 200 280 112 196 2.59e-11 65.5
MS.gene60590.t1 MTR_1g053800 52.381 63 28 1 209 269 175 237 3.02e-11 64.3
MS.gene60590.t1 MTR_3g086100 40.698 86 45 2 200 280 183 267 4.23e-11 64.7
MS.gene60590.t1 MTR_0450s0040 43.243 74 41 1 210 283 168 240 4.34e-11 63.9
MS.gene60590.t1 MTR_7g093030 44.776 67 37 0 211 277 212 278 7.99e-11 62.8
MS.gene60590.t1 MTR_7g115530 57.692 52 22 0 211 262 218 269 8.11e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60590.t1 AT1G68670 48.521 338 124 12 10 326 16 324 7.77e-81 251
MS.gene60590.t1 AT1G25550 46.687 332 124 11 10 328 20 311 1.75e-78 245
MS.gene60590.t1 AT3G25790 44.022 368 164 10 10 372 20 350 4.97e-76 239
MS.gene60590.t1 AT1G13300 48.123 293 104 8 11 299 20 268 1.27e-71 228
MS.gene60590.t1 AT1G25550 49.615 260 103 8 10 261 20 259 1.44e-67 215
MS.gene60590.t1 AT3G25790 46.457 254 109 4 9 261 19 246 6.06e-58 189
MS.gene60590.t1 AT2G03500 35.238 315 140 8 8 294 36 314 1.86e-47 167
MS.gene60590.t1 AT4G37180 34.296 277 108 8 11 269 48 268 8.45e-35 131
MS.gene60590.t1 AT4G37180 33.935 277 116 8 11 269 48 275 3.68e-32 124
MS.gene60590.t1 AT1G49560 32.542 295 132 9 10 293 30 268 1.24e-30 119
MS.gene60590.t1 AT4G37180 33.457 269 105 8 11 261 48 260 3.10e-30 117
MS.gene60590.t1 AT1G49560 31.939 263 118 8 10 261 30 242 1.43e-23 99.0
MS.gene60590.t1 AT2G01060 59.375 64 26 0 211 274 15 78 1.57e-16 79.7
MS.gene60590.t1 AT3G16857 35.252 139 79 3 199 336 155 283 1.34e-14 75.9
MS.gene60590.t1 AT3G16857 34.752 141 81 3 199 338 225 355 1.75e-14 75.5
MS.gene60590.t1 AT3G16857 34.752 141 81 3 199 338 225 355 1.80e-14 75.5
MS.gene60590.t1 AT2G25180 32.515 163 93 5 209 356 193 353 3.54e-14 74.3
MS.gene60590.t1 AT5G58080 50.000 66 32 1 204 269 187 251 8.83e-14 73.2
MS.gene60590.t1 AT1G79430 61.818 55 21 0 211 265 34 88 1.07e-13 72.0
MS.gene60590.t1 AT4G13640 60.000 55 22 0 211 265 37 91 1.23e-13 71.2
MS.gene60590.t1 AT4G13640 60.000 55 22 0 211 265 37 91 1.25e-13 71.2
MS.gene60590.t1 AT3G24120 60.000 55 22 0 211 265 41 95 1.29e-13 71.2
MS.gene60590.t1 AT3G24120 60.000 55 22 0 211 265 41 95 1.38e-13 71.2
MS.gene60590.t1 AT3G13040 41.935 93 42 1 178 270 220 300 2.59e-13 71.2
MS.gene60590.t1 AT3G13040 41.935 93 42 1 178 270 220 300 2.59e-13 71.2
MS.gene60590.t1 AT3G13040 41.935 93 42 1 178 270 220 300 2.59e-13 71.2
MS.gene60590.t1 AT5G05090 48.611 72 35 2 199 269 68 138 4.43e-13 69.3
MS.gene60590.t1 AT3G04030 52.308 65 31 0 211 275 45 109 4.67e-13 70.5
MS.gene60590.t1 AT3G12730 58.182 55 23 0 211 265 23 77 4.98e-13 68.6
MS.gene60590.t1 AT3G04030 52.308 65 31 0 211 275 45 109 5.25e-13 70.1
MS.gene60590.t1 AT2G01760 44.156 77 42 1 210 286 134 209 7.91e-13 68.9
MS.gene60590.t1 AT5G59570 39.252 107 63 2 163 269 94 198 8.58e-13 68.9
MS.gene60590.t1 AT5G59570 39.252 107 63 2 163 269 94 198 8.58e-13 68.9
MS.gene60590.t1 AT5G18240 58.182 55 23 0 211 265 45 99 9.39e-13 69.3
MS.gene60590.t1 AT5G18240 58.182 55 23 0 211 265 45 99 9.39e-13 69.3
MS.gene60590.t1 AT2G42660 54.386 57 26 0 216 272 55 111 9.51e-13 68.2
MS.gene60590.t1 AT2G01760 44.156 77 42 1 210 286 227 302 9.70e-13 69.3
MS.gene60590.t1 AT5G18240 58.182 55 23 0 211 265 45 99 9.88e-13 69.3
MS.gene60590.t1 AT5G18240 58.182 55 23 0 211 265 45 99 1.02e-12 69.3
MS.gene60590.t1 AT5G18240 58.182 55 23 0 211 265 45 99 1.02e-12 69.3
MS.gene60590.t1 AT2G01760 44.156 77 42 1 210 286 199 274 1.11e-12 69.3
MS.gene60590.t1 AT5G44190 43.750 80 44 1 200 279 136 214 1.40e-12 68.6
MS.gene60590.t1 AT2G06020 52.727 55 26 0 211 265 86 140 1.64e-12 68.2
MS.gene60590.t1 AT5G44190 43.750 80 44 1 200 279 136 214 1.67e-12 68.6
MS.gene60590.t1 AT2G40970 45.070 71 38 1 199 269 93 162 1.69e-12 67.4
MS.gene60590.t1 AT3G46640 33.113 151 92 4 134 276 59 208 1.76e-12 68.2
MS.gene60590.t1 AT5G06800 51.613 62 30 0 211 272 192 253 2.01e-12 67.4
MS.gene60590.t1 AT3G10760 50.000 60 29 1 210 269 104 162 2.22e-12 67.8
MS.gene60590.t1 AT3G46640 46.269 67 35 1 210 276 143 208 2.53e-12 67.8
MS.gene60590.t1 AT3G46640 46.269 67 35 1 210 276 143 208 2.53e-12 67.8
MS.gene60590.t1 AT3G04450 51.667 60 29 0 211 270 238 297 4.53e-12 66.6
MS.gene60590.t1 AT5G45580 43.243 74 42 0 211 284 7 80 5.43e-12 65.9
MS.gene60590.t1 AT5G45580 43.243 74 42 0 211 284 23 96 5.52e-12 66.2
MS.gene60590.t1 AT5G06800 51.613 62 30 0 211 272 192 253 6.37e-12 67.0
MS.gene60590.t1 AT5G29000 49.206 63 32 0 211 273 231 293 6.39e-12 66.2
MS.gene60590.t1 AT5G06800 51.613 62 30 0 211 272 192 253 6.47e-12 66.6
MS.gene60590.t1 AT1G69580 54.545 55 25 0 211 265 34 88 7.50e-12 66.2
MS.gene60590.t1 AT1G69580 54.545 55 25 0 211 265 34 88 7.90e-12 66.2
MS.gene60590.t1 AT3G04450 51.667 60 29 0 211 270 238 297 8.49e-12 66.6
MS.gene60590.t1 AT3G04450 51.667 60 29 0 211 270 198 257 9.84e-12 66.2
MS.gene60590.t1 AT3G04450 51.667 60 29 0 211 270 198 257 9.84e-12 66.2
MS.gene60590.t1 AT2G20570 47.945 73 37 1 207 279 149 220 1.05e-11 66.2
MS.gene60590.t1 AT5G29000 49.206 63 32 0 211 273 231 293 1.36e-11 65.9
MS.gene60590.t1 AT4G28610 51.667 60 29 0 211 270 225 284 1.38e-11 65.9
MS.gene60590.t1 AT5G29000 49.206 63 32 0 211 273 188 250 1.43e-11 65.9
MS.gene60590.t1 AT5G29000 49.206 63 32 0 211 273 188 250 1.43e-11 65.9
MS.gene60590.t1 AT2G40260 57.692 52 22 0 213 264 84 135 1.49e-11 65.9
MS.gene60590.t1 AT2G38300 51.923 52 25 0 213 264 56 107 2.06e-11 65.1
MS.gene60590.t1 AT3G04030 50.769 65 31 1 211 275 45 108 2.87e-11 65.1
MS.gene60590.t1 AT4G31920 42.105 76 43 1 204 279 176 250 4.91e-11 64.7
MS.gene60590.t1 AT4G04605 51.923 52 25 0 213 264 16 67 6.23e-11 62.0

Find 78 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTAACTCTATCTTCTTTA+TGG 0.063110 1.2:+69318270 None:intergenic
CACAATGCAGCGTTTCAAAA+TGG 0.179894 1.2:-69319007 None:intergenic
ACAAAAGTGGTTGCACCTTC+TGG 0.247597 1.2:-69317454 MS.gene60590:CDS
CATATCTACAAACCTGTAAA+TGG 0.279375 1.2:+69317861 None:intergenic
TGAAGTCAAAAGCCATTTAC+AGG 0.284068 1.2:-69317873 MS.gene60590:intron
AACGAAAGAGGAAGTTCTTT+AGG 0.297245 1.2:+69318928 None:intergenic
GATGTCTCCAACATTGTTAC+TGG 0.321828 1.2:+69318166 None:intergenic
ATTACCTCTCTGATTTGCTT+TGG 0.356836 1.2:+69317920 None:intergenic
GTCGTTCTTGTTCTAATGCT+TGG 0.361767 1.2:+69318968 None:intergenic
CTTTCGTTATTTGTTGAACT+TGG 0.382926 1.2:+69317580 None:intergenic
GGCTTAGATCTGTTCAGTTA+TGG 0.389861 1.2:-69318526 MS.gene60590:CDS
CCCCACCATACTCTTGTTGT+TGG 0.395076 1.2:+69317506 None:intergenic
TTTGTCCAACAACAAGAGTA+TGG 0.414336 1.2:-69317511 MS.gene60590:CDS
ATTATTACCTCTTCAGATGA+AGG 0.417768 1.2:+69318494 None:intergenic
ACAATGCAGCGTTTCAAAAT+GGG 0.423030 1.2:-69319006 MS.gene60590:CDS
GTAGCTTCATCAACAACAAC+AGG 0.429695 1.2:-69317484 MS.gene60590:CDS
GTGTTAGAATTGAAGAGAAA+TGG 0.430861 1.2:-69318320 MS.gene60590:CDS
TGCTCAAACCTCTTGAACTC+TGG 0.432408 1.2:+69317382 None:intergenic
GCAGCGACGGTGCTGGTCGC+AGG 0.434166 1.2:-69318114 MS.gene60590:CDS
GTTTATTCCAATTAAGAAAA+GGG 0.437351 1.2:-69318654 MS.gene60590:CDS
GTTGCACAAGCGATTCTTGA+AGG 0.440339 1.2:-69318090 MS.gene60590:CDS
AGTTTATTCCAATTAAGAAA+AGG 0.446516 1.2:-69318655 MS.gene60590:CDS
ACTCTATCTTCTTTATGGAA+TGG 0.449526 1.2:+69318275 None:intergenic
GCTTGTCGACAACAATTGTC+TGG 0.459321 1.2:-69318758 MS.gene60590:CDS
CATCAAAAGAGACGAGAAAG+AGG 0.461312 1.2:-69318147 MS.gene60590:CDS
TTGAAGGCGCTTCAGCAACT+TGG 0.469776 1.2:-69318074 MS.gene60590:CDS
TATCTTCTTTATGGAATGGA+TGG 0.476618 1.2:+69318279 None:intergenic
AGAGAGGTAATGAATGTTGA+TGG 0.478733 1.2:-69317908 MS.gene60590:CDS
GCATTCAATCTCAGATGAAA+GGG 0.480171 1.2:-69317362 MS.gene60590:CDS
AGGTGCTGCTGTTTGTGAAT+TGG 0.480961 1.2:+69317555 None:intergenic
CTCTCTGATTTGCTTTGGTG+TGG 0.488332 1.2:+69317925 None:intergenic
ACAACTGAGTATAATTTGAA+TGG 0.489356 1.2:-69318731 MS.gene60590:CDS
TCAATAACCAGGGGAAGTAT+TGG 0.498150 1.2:+69317270 None:intergenic
TTGGTCTTCTAGTATGCAAC+CGG 0.498211 1.2:+69317599 None:intergenic
ACAAGTGAATTGTTAAGTAA+AGG 0.498649 1.2:-69318242 MS.gene60590:CDS
ATGTTGCCTACAAGGACAAA+AGG 0.503411 1.2:+69317535 None:intergenic
AAGGCGCTTCAGCAACTTGG+TGG 0.504237 1.2:-69318071 MS.gene60590:CDS
TTAATAAACCAGAGTTCAAG+AGG 0.515038 1.2:-69317390 MS.gene60590:CDS
ACTTGAGGATGTGTGGCTAC+TGG 0.518302 1.2:+69317421 None:intergenic
AGCATTCAATCTCAGATGAA+AGG 0.522044 1.2:-69317363 MS.gene60590:CDS
AAGAGAAATGGAAGTGGTGG+TGG 0.523432 1.2:-69318308 MS.gene60590:CDS
TCCAACAACAAGAGTATGGT+GGG 0.538170 1.2:-69317507 MS.gene60590:CDS
AGAGAAAGCAGCGACGGTGC+TGG 0.540249 1.2:-69318121 MS.gene60590:CDS
AGAACTCACGGCAGCTACAC+CGG 0.545612 1.2:+69318205 None:intergenic
TGTGTAGAGGATGAAGATGT+AGG 0.546735 1.2:+69317304 None:intergenic
TTATTAATTGTAGCAACTTG+AGG 0.553389 1.2:+69317406 None:intergenic
GTAACGAGTTCTAACGAAAG+AGG 0.565892 1.2:+69318916 None:intergenic
TTTCTCTTCAATTCTAACAC+AGG 0.568919 1.2:+69318323 None:intergenic
GGCAGCTACACCGGTCGAAG+AGG 0.574491 1.2:+69318214 None:intergenic
AAAATTAAAACTCAATAACC+AGG 0.575545 1.2:+69317259 None:intergenic
AGGAAAAGAGAAAGCAGCGA+CGG 0.577313 1.2:-69318127 MS.gene60590:CDS
ACTACGGCCAATACTTCCCC+TGG 0.578329 1.2:-69317277 MS.gene60590:CDS
TTCAGATCCTTCATCTGAAG+AGG 0.584122 1.2:-69318501 MS.gene60590:intron
ATAATAAGAAGAAATCTGAC+TGG 0.585753 1.2:-69318547 MS.gene60590:CDS
AAAGGACAACCCTCTTCGAC+CGG 0.587220 1.2:-69318224 MS.gene60590:CDS
AAATTAAAACTCAATAACCA+GGG 0.590216 1.2:+69317260 None:intergenic
GCAGCTACACCGGTCGAAGA+GGG 0.597437 1.2:+69318215 None:intergenic
AACAGGGGAAATGACAAAAG+TGG 0.604173 1.2:-69317467 MS.gene60590:CDS
ATCCTCTACACATACCACTA+CGG 0.615767 1.2:-69317293 MS.gene60590:CDS
AGGGGAAGTATTGGCCGTAG+TGG 0.617858 1.2:+69317279 None:intergenic
GTCCAACAACAAGAGTATGG+TGG 0.618792 1.2:-69317508 MS.gene60590:CDS
ACTGTCACCAGTAACAATGT+TGG 0.620417 1.2:-69318173 MS.gene60590:CDS
GAACAGACAACATCAACAGA+TGG 0.623810 1.2:-69318692 MS.gene60590:CDS
AGTAGCCGCATTAGAACTCA+CGG 0.629986 1.2:+69318193 None:intergenic
TCGTTAGAACTCGTTACACA+AGG 0.638093 1.2:-69318910 MS.gene60590:intron
GAAAGGGGTAATAACAGTGA+AGG 0.640994 1.2:-69317346 MS.gene60590:CDS
GGCCGTAGTGGTATGTGTAG+AGG 0.652196 1.2:+69317291 None:intergenic
CATTCAATCTCAGATGAAAG+GGG 0.655574 1.2:-69317361 MS.gene60590:CDS
ACTGGTGCATAGATTCCAGA+AGG 0.656305 1.2:+69317439 None:intergenic
GAATTGAAGAGAAATGGAAG+TGG 0.663638 1.2:-69318314 MS.gene60590:CDS
TTGAAGAGAAATGGAAGTGG+TGG 0.664227 1.2:-69318311 MS.gene60590:CDS
AGCTGCCGTGAGTTCTAATG+CGG 0.671481 1.2:-69318198 MS.gene60590:CDS
TGTAGCAACTTGAGGATGTG+TGG 0.683531 1.2:+69317414 None:intergenic
TAGCTTCATCAACAACAACA+GGG 0.693117 1.2:-69317483 MS.gene60590:CDS
AATTAAAACTCAATAACCAG+GGG 0.700665 1.2:+69317261 None:intergenic
CCAACAACAAGAGTATGGTG+GGG 0.702776 1.2:-69317506 MS.gene60590:CDS
AGCTTCATCAACAACAACAG+GGG 0.709783 1.2:-69317482 MS.gene60590:CDS
CACACCAAAGCAAATCAGAG+AGG 0.720773 1.2:-69317924 MS.gene60590:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATATAAAAAAAAAACAAAA+AGG + chr1.2:69317400-69317419 None:intergenic 10.0%
!!! AAAAAAATTGATTTTAATTG+AGG + chr1.2:69318630-69318649 None:intergenic 10.0%
!!! AAAATAATTTTGGATTTTTT+TGG - chr1.2:69317473-69317492 MS.gene60590:CDS 10.0%
!!! TTTGTATTTTTTTTTTATCT+GGG - chr1.2:69317434-69317453 MS.gene60590:CDS 10.0%
!!! GTTTGTATTTTTTTTTTATC+TGG - chr1.2:69317433-69317452 MS.gene60590:CDS 15.0%
!! AGTTTATTCCAATTAAGAAA+AGG - chr1.2:69317618-69317637 MS.gene60590:intron 20.0%
!! GTTTATTCCAATTAAGAAAA+GGG - chr1.2:69317619-69317638 MS.gene60590:intron 20.0%
!! TTGCTTTCTGTTAAATTAAA+GGG - chr1.2:69317850-69317869 MS.gene60590:intron 20.0%
!! TTTGCTTTCTGTTAAATTAA+AGG - chr1.2:69317849-69317868 MS.gene60590:intron 20.0%
! AAAATTGACAACAAAAGAGT+TGG + chr1.2:69318447-69318466 None:intergenic 25.0%
! AAATTGACAACAAAAGAGTT+GGG + chr1.2:69318446-69318465 None:intergenic 25.0%
! AATTGAGGATATTAAACTGT+TGG + chr1.2:69318615-69318634 None:intergenic 25.0%
! ACAACTGAGTATAATTTGAA+TGG - chr1.2:69317542-69317561 MS.gene60590:CDS 25.0%
! ACAAGTGAATTGTTAAGTAA+AGG - chr1.2:69318031-69318050 MS.gene60590:intron 25.0%
! ATAATAAGAAGAAATCTGAC+TGG - chr1.2:69317726-69317745 MS.gene60590:intron 25.0%
! ATATTAAGATACTCACAATC+TGG + chr1.2:69318229-69318248 None:intergenic 25.0%
! GTTTGTGTGAAAAATTGTTT+AGG - chr1.2:69317907-69317926 MS.gene60590:CDS 25.0%
! TAAGCAACAAAAAGTTTCTT+TGG - chr1.2:69317703-69317722 MS.gene60590:intron 25.0%
! TGTTAACTCTATCTTCTTTA+TGG + chr1.2:69318006-69318025 None:intergenic 25.0%
! TTATTAATTGTAGCAACTTG+AGG + chr1.2:69318870-69318889 None:intergenic 25.0%
!! TTTTTCACACAAACAAAACA+AGG + chr1.2:69317901-69317920 None:intergenic 25.0%
ACTCTATCTTCTTTATGGAA+TGG + chr1.2:69318001-69318020 None:intergenic 30.0%
ATTATTACCTCTTCAGATGA+AGG + chr1.2:69317782-69317801 None:intergenic 30.0%
CATATCTACAAACCTGTAAA+TGG + chr1.2:69318415-69318434 None:intergenic 30.0%
GAAATCCTATGCAATTGAAA+GGG + chr1.2:69318551-69318570 None:intergenic 30.0%
GGAATAAACTCTTCAAAAAC+AGG + chr1.2:69317608-69317627 None:intergenic 30.0%
GTGTTAGAATTGAAGAGAAA+TGG - chr1.2:69317953-69317972 MS.gene60590:intron 30.0%
TATCTTCTTTATGGAATGGA+TGG + chr1.2:69317997-69318016 None:intergenic 30.0%
TGAAATCCTATGCAATTGAA+AGG + chr1.2:69318552-69318571 None:intergenic 30.0%
TTAATAAACCAGAGTTCAAG+AGG - chr1.2:69318883-69318902 MS.gene60590:intron 30.0%
TTTCTCTTCAATTCTAACAC+AGG + chr1.2:69317953-69317972 None:intergenic 30.0%
! CTTTCGTTATTTGTTGAACT+TGG + chr1.2:69318696-69318715 None:intergenic 30.0%
!!! GTTTTTTGCTTCTGTTCTAT+AGG - chr1.2:69318323-69318342 MS.gene60590:CDS 30.0%
!!! TTTTGACTTCATCATTTGTG+AGG + chr1.2:69318391-69318410 None:intergenic 30.0%
AACGAAAGAGGAAGTTCTTT+AGG + chr1.2:69317348-69317367 None:intergenic 35.0%
AGAGAGGTAATGAATGTTGA+TGG - chr1.2:69318365-69318384 MS.gene60590:intron 35.0%
AGCATTCAATCTCAGATGAA+AGG - chr1.2:69318910-69318929 MS.gene60590:intron 35.0%
CATTCAATCTCAGATGAAAG+GGG - chr1.2:69318912-69318931 MS.gene60590:CDS 35.0%
GAATTGAAGAGAAATGGAAG+TGG - chr1.2:69317959-69317978 MS.gene60590:intron 35.0%
GCATTCAATCTCAGATGAAA+GGG - chr1.2:69318911-69318930 MS.gene60590:CDS 35.0%
TAGCTTCATCAACAACAACA+GGG - chr1.2:69318790-69318809 MS.gene60590:intron 35.0%
TGAAGTCAAAAGCCATTTAC+AGG - chr1.2:69318400-69318419 MS.gene60590:intron 35.0%
TTAGTCCCTTTCAATTGCAT+AGG - chr1.2:69318543-69318562 MS.gene60590:CDS 35.0%
TTTGTCCAACAACAAGAGTA+TGG - chr1.2:69318762-69318781 MS.gene60590:CDS 35.0%
! GAAGAAGCCCTTTTCTTAAT+TGG + chr1.2:69317629-69317648 None:intergenic 35.0%
! TCTGTTTTGCACAGAAATAC+CGG - chr1.2:69318655-69318674 MS.gene60590:CDS 35.0%
!! ATTACCTCTCTGATTTGCTT+TGG + chr1.2:69318356-69318375 None:intergenic 35.0%
AACAGGGGAAATGACAAAAG+TGG - chr1.2:69318806-69318825 MS.gene60590:intron 40.0%
ACTGTCACCAGTAACAATGT+TGG - chr1.2:69318100-69318119 MS.gene60590:CDS 40.0%
AGCTTCATCAACAACAACAG+GGG - chr1.2:69318791-69318810 MS.gene60590:intron 40.0%
ATCCTCTACACATACCACTA+CGG - chr1.2:69318980-69318999 MS.gene60590:CDS 40.0%
ATGTTGCCTACAAGGACAAA+AGG + chr1.2:69318741-69318760 None:intergenic 40.0%
CATCAAAAGAGACGAGAAAG+AGG - chr1.2:69318126-69318145 MS.gene60590:CDS 40.0%
GAAAGGGGTAATAACAGTGA+AGG - chr1.2:69318927-69318946 MS.gene60590:CDS 40.0%
GAACAGACAACATCAACAGA+TGG - chr1.2:69317581-69317600 MS.gene60590:CDS 40.0%
GATGTCTCCAACATTGTTAC+TGG + chr1.2:69318110-69318129 None:intergenic 40.0%
GGACAAAAATGTTGCCTACA+AGG + chr1.2:69318749-69318768 None:intergenic 40.0%
GGCTTAGATCTGTTCAGTTA+TGG - chr1.2:69317747-69317766 MS.gene60590:intron 40.0%
GTAGCTTCATCAACAACAAC+AGG - chr1.2:69318789-69318808 MS.gene60590:intron 40.0%
GTCGTTCTTGTTCTAATGCT+TGG + chr1.2:69317308-69317327 None:intergenic 40.0%
TCGTTAGAACTCGTTACACA+AGG - chr1.2:69317363-69317382 MS.gene60590:CDS 40.0%
TGTGTAGAGGATGAAGATGT+AGG + chr1.2:69318972-69318991 None:intergenic 40.0%
TTCAGATCCTTCATCTGAAG+AGG - chr1.2:69317772-69317791 MS.gene60590:intron 40.0%
TTGAAGAGAAATGGAAGTGG+TGG - chr1.2:69317962-69317981 MS.gene60590:intron 40.0%
! GTAACGAGTTCTAACGAAAG+AGG + chr1.2:69317360-69317379 None:intergenic 40.0%
! TCAATAACCAGGGGAAGTAT+TGG + chr1.2:69319006-69319025 None:intergenic 40.0%
! TCCAACAACAAGAGTATGGT+GGG - chr1.2:69318766-69318785 MS.gene60590:CDS 40.0%
! TTGGTCTTCTAGTATGCAAC+CGG + chr1.2:69318677-69318696 None:intergenic 40.0%
AAGAGAAATGGAAGTGGTGG+TGG - chr1.2:69317965-69317984 MS.gene60590:intron 45.0%
ACAAAAGTGGTTGCACCTTC+TGG - chr1.2:69318819-69318838 MS.gene60590:intron 45.0%
ACTGGTGCATAGATTCCAGA+AGG + chr1.2:69318837-69318856 None:intergenic 45.0%
AGGAAAAGAGAAAGCAGCGA+CGG - chr1.2:69318146-69318165 MS.gene60590:CDS 45.0%
AGTAGCCGCATTAGAACTCA+CGG + chr1.2:69318083-69318102 None:intergenic 45.0%
CACACCAAAGCAAATCAGAG+AGG - chr1.2:69318349-69318368 MS.gene60590:intron 45.0%
GCTTGTCGACAACAATTGTC+TGG - chr1.2:69317515-69317534 MS.gene60590:CDS 45.0%
GTTGCACAAGCGATTCTTGA+AGG - chr1.2:69318183-69318202 MS.gene60590:CDS 45.0%
TGCTCAAACCTCTTGAACTC+TGG + chr1.2:69318894-69318913 None:intergenic 45.0%
TGTAGCAACTTGAGGATGTG+TGG + chr1.2:69318862-69318881 None:intergenic 45.0%
! CCAACAACAAGAGTATGGTG+GGG - chr1.2:69318767-69318786 MS.gene60590:CDS 45.0%
! GTCCAACAACAAGAGTATGG+TGG - chr1.2:69318765-69318784 MS.gene60590:CDS 45.0%
!! AGGTGCTGCTGTTTGTGAAT+TGG + chr1.2:69318721-69318740 None:intergenic 45.0%
!! CTCTCTGATTTGCTTTGGTG+TGG + chr1.2:69318351-69318370 None:intergenic 45.0%
!! ATATAAAAAAAAAACAAAAA+GGG + chr1.2:69317399-69317418 None:intergenic 5.0%
!!! TTATTTTTGAAAAATAATTT+TGG - chr1.2:69317463-69317482 MS.gene60590:CDS 5.0%
AAAGGACAACCCTCTTCGAC+CGG - chr1.2:69318049-69318068 MS.gene60590:intron 50.0%
ACTTGAGGATGTGTGGCTAC+TGG + chr1.2:69318855-69318874 None:intergenic 50.0%
! AGCTGCCGTGAGTTCTAATG+CGG - chr1.2:69318075-69318094 MS.gene60590:CDS 50.0%
! CCCCACCATACTCTTGTTGT+TGG + chr1.2:69318770-69318789 None:intergenic 50.0%
! GCAGCACCTTTTGTCCTTGT+AGG - chr1.2:69318732-69318751 MS.gene60590:CDS 50.0%
!! TTGAAGGCGCTTCAGCAACT+TGG - chr1.2:69318199-69318218 MS.gene60590:CDS 50.0%
ACTACGGCCAATACTTCCCC+TGG - chr1.2:69318996-69319015 MS.gene60590:CDS 55.0%
AGAACTCACGGCAGCTACAC+CGG + chr1.2:69318071-69318090 None:intergenic 55.0%
GGCCGTAGTGGTATGTGTAG+AGG + chr1.2:69318985-69319004 None:intergenic 55.0%
! AAGGCGCTTCAGCAACTTGG+TGG - chr1.2:69318202-69318221 MS.gene60590:CDS 55.0%
!! AGGGGAAGTATTGGCCGTAG+TGG + chr1.2:69318997-69319016 None:intergenic 55.0%
AGAGAAAGCAGCGACGGTGC+TGG - chr1.2:69318152-69318171 MS.gene60590:CDS 60.0%
GCAGCTACACCGGTCGAAGA+GGG + chr1.2:69318061-69318080 None:intergenic 60.0%
GGCAGCTACACCGGTCGAAG+AGG + chr1.2:69318062-69318081 None:intergenic 65.0%
!! GCAGCGACGGTGCTGGTCGC+AGG - chr1.2:69318159-69318178 MS.gene60590:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 69317270 69319025 69317270 ID=MS.gene60590
chr1.2 mRNA 69317270 69319025 69317270 ID=MS.gene60590.t1;Parent=MS.gene60590
chr1.2 exon 69318911 69319025 69318911 ID=MS.gene60590.t1.exon1;Parent=MS.gene60590.t1
chr1.2 CDS 69318911 69319025 69318911 ID=cds.MS.gene60590.t1;Parent=MS.gene60590.t1
chr1.2 exon 69318502 69318788 69318502 ID=MS.gene60590.t1.exon2;Parent=MS.gene60590.t1
chr1.2 CDS 69318502 69318788 69318502 ID=cds.MS.gene60590.t1;Parent=MS.gene60590.t1
chr1.2 exon 69318063 69318366 69318063 ID=MS.gene60590.t1.exon3;Parent=MS.gene60590.t1
chr1.2 CDS 69318063 69318366 69318063 ID=cds.MS.gene60590.t1;Parent=MS.gene60590.t1
chr1.2 exon 69317874 69317950 69317874 ID=MS.gene60590.t1.exon4;Parent=MS.gene60590.t1
chr1.2 CDS 69317874 69317950 69317874 ID=cds.MS.gene60590.t1;Parent=MS.gene60590.t1
chr1.2 exon 69317270 69317626 69317270 ID=MS.gene60590.t1.exon5;Parent=MS.gene60590.t1
chr1.2 CDS 69317270 69317626 69317270 ID=cds.MS.gene60590.t1;Parent=MS.gene60590.t1
Gene Sequence

>MS.gene60590

ATGCAGCGTTTCAAAATGGGTTTCTCTGATTATATCCAAGCATTAGAACAAGAACGACGTAAGATTCAAGTCTTTCCTAAAGAACTTCCTCTTTCGTTAGAACTCGTTACACAAGGTACGTACATACCCTTTTTGTTTTTTTTTTATATGATTATGAGTTAAAGTTTGTATTTTTTTTTTATCTGGGTTTGTTTTATTTTTGAAAAATAATTTTGGATTTTTTTGGTTGATTATTAGCTATTGAAGCTTGTCGACAACAATTGTCTGGAACAACAACTGAGTATAATTTGAATGGACAATCTGAGTGTTCTGAACAGACAACATCAACAGATGGACCTGTTTTTGAAGAGTTTATTCCAATTAAGAAAAGGGCTTCTTCTTATTCTCAAGAAGTGTTTGATGATGTAGAAGATTATGATCAACATTTTCAGCATAAGCAACAAAAAGTTTCTTTGGATAATAAGAAGAAATCTGACTGGCTTAGATCTGTTCAGTTATGGAATTCAGATCCTTCATCTGAAGAGGTAATAATAAAATCATAGCTTAATTTTCAATTATTGTTAATTGTTGTTTTAGTCTTTTGCTTTCTGTTAAATTAAAGGGAACAAACAATGAATTGAGTAATTGACCTTGTTTTGTTTGTGTGAAAAATTGTTTAGGATGTTACTAAGAAAGCAGTGCCTGTGTTAGAATTGAAGAGAAATGGAAGTGGTGGTGGTGCTTTCCATCCATTCCATAAAGAAGATAGAGTTAACAACAAGACAAGTGAATTGTTAAGTAAAGGACAACCCTCTTCGACCGGTGTAGCTGCCGTGAGTTCTAATGCGGCTACTGTCACCAGTAACAATGTTGGAGACATCAAAAGAGACGAGAAAGAGGAAAAGAGAAAGCAGCGACGGTGCTGGTCGCAGGAGTTGCACAAGCGATTCTTGAAGGCGCTTCAGCAACTTGGTGGTCCAGATTGTGAGTATCTTAATATATACATCAAGTTCTGATTGTTATGTATACATGTTGTTAATGTAATTAACTGCAAATAGAATAAATGTTTAATGTGTTTTTTGCTTCTGTTCTATAGGTGCCACACCAAAGCAAATCAGAGAGGTAATGAATGTTGATGGCCTCACAAATGATGAAGTCAAAAGCCATTTACAGGTTTGTAGATATGTTCTTCTTCCCAACTCTTTTGTTGTCAATTTTTATCTGAGTTTTTGTTTTGATTTTGTGATTTCTATTATCTAGTTGAATCTTTTAGTTTTAACTGTTTCATCTTGTTTTAGTCCCTTTCAATTGCATAGGATTTCAAAACAAACGAGTGATAGTTTTTGTTGTTACATAAGTTACACCAACAGTTTAATATCCTCAATTAAAATCAATTTTTTTTTCTTTCTGTTTTGCACAGAAATACCGGTTGCATACTAGAAGACCAAGTTCAACAAATAACGAAAGTGCCAATTCACAAACAGCAGCACCTTTTGTCCTTGTAGGCAACATTTTTGTCCAACAACAAGAGTATGGTGGGGTAGCTTCATCAACAACAACAGGGGAAATGACAAAAGTGGTTGCACCTTCTGGAATCTATGCACCAGTAGCCACACATCCTCAAGTTGCTACAATTAATAAACCAGAGTTCAAGAGGTTTGAGCATTCAATCTCAGATGAAAGGGGTAATAACAGTGAAGGTGCTGTTCATTCAAATTCACCTACATCTTCATCCTCTACACATACCACTACGGCCAATACTTCCCCTGGTTATTGA
Protein sequence

>MS.gene60590.t1

MQRFKMGFSDYIQALEQERRKIQVFPKELPLSLELVTQAIEACRQQLSGTTTEYNLNGQSECSEQTTSTDGPVFEEFIPIKKRASSYSQEVFDDVEDYDQHFQHKQQKVSLDNKKKSDWLRSVQLWNSDPSSEEDVTKKAVPVLELKRNGSGGGAFHPFHKEDRVNNKTSELLSKGQPSSTGVAAVSSNAATVTSNNVGDIKRDEKEEKRKQRRCWSQELHKRFLKALQQLGGPDCATPKQIREVMNVDGLTNDEVKSHLQKYRLHTRRPSSTNNESANSQTAAPFVLVGNIFVQQQEYGGVASSTTTGEMTKVVAPSGIYAPVATHPQVATINKPEFKRFEHSISDERGNNSEGAVHSNSPTSSSSTHTTTANTSPGY