AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60638

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60638.t1 MTR_0558s0020 77.027 296 38 2 1 266 1 296 1.61e-159 461
MS.gene60638.t1 MTR_1g083640 72.183 284 34 2 1 239 1 284 3.77e-139 409
MS.gene60638.t1 MTR_8g446020 64.068 295 38 2 1 266 1 256 3.36e-121 362
MS.gene60638.t1 MTR_2g014100 69.796 245 36 2 1 207 1 245 1.40e-110 334
MS.gene60638.t1 MTR_3g008240 81.910 199 35 1 1 198 1 199 5.58e-110 331
MS.gene60638.t1 MTR_6g090505 70.464 237 41 1 65 272 1 237 7.84e-110 337
MS.gene60638.t1 MTR_6g090505 67.647 102 6 2 367 466 230 306 6.16e-34 134
MS.gene60638.t1 MTR_6g090505 79.592 49 10 0 538 586 314 362 2.66e-12 69.3
MS.gene60638.t1 MTR_4g040430 80.303 198 35 1 1 198 1 194 1.56e-107 324
MS.gene60638.t1 MTR_0337s0040 60.769 260 33 3 1 230 1 221 3.68e-92 286
MS.gene60638.t1 MTR_0337s0060 56.727 275 22 3 418 692 21 198 2.68e-90 281
MS.gene60638.t1 MTR_5g071590 72.222 180 37 2 1 167 1 180 5.53e-87 271
MS.gene60638.t1 MTR_1g055240 84.259 108 17 0 91 198 3 110 7.04e-59 196
MS.gene60638.t1 MTR_4g133495 74.603 126 29 1 547 669 88 213 1.38e-58 199
MS.gene60638.t1 MTR_4g133340 74.603 126 29 1 547 669 88 213 1.38e-58 199
MS.gene60638.t1 MTR_1281s0010 79.817 109 22 0 19 127 1 109 5.56e-56 186
MS.gene60638.t1 MTR_0019s0290 63.158 152 27 1 82 204 9 160 9.31e-56 187
MS.gene60638.t1 MTR_3g028680 82.692 104 18 0 589 692 1 104 4.10e-54 184
MS.gene60638.t1 MTR_2g067090 79.208 101 21 0 592 692 99 199 7.40e-48 171
MS.gene60638.t1 MTR_4g008820 69.000 100 30 1 1 99 125 224 6.49e-31 129
MS.gene60638.t1 MTR_8g046060 76.136 88 12 2 1 79 91 178 1.29e-30 120
MS.gene60638.t1 MTR_6g051425 29.560 318 164 10 40 312 22 324 2.07e-27 114
MS.gene60638.t1 MTR_0337s0050 75.000 36 9 0 230 265 14 49 2.21e-13 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60638.t1 AT4G38180 25.960 651 384 14 16 636 55 637 5.04e-61 219
MS.gene60638.t1 AT4G15090 26.946 501 306 8 192 689 218 661 1.38e-56 207
MS.gene60638.t1 AT4G15090 26.946 501 306 8 192 689 218 661 1.38e-56 207
MS.gene60638.t1 AT4G15090 26.946 501 306 8 192 689 218 661 1.38e-56 207
MS.gene60638.t1 AT4G15090 26.946 501 306 8 192 689 218 661 1.38e-56 207
MS.gene60638.t1 AT4G15090 26.946 501 306 8 192 689 218 661 1.38e-56 207
MS.gene60638.t1 AT3G06250 25.493 608 351 16 34 632 189 703 4.77e-51 190
MS.gene60638.t1 AT3G06250 25.493 608 351 16 34 632 189 703 4.77e-51 190
MS.gene60638.t1 AT3G06250 25.493 608 351 16 34 632 189 703 4.77e-51 190
MS.gene60638.t1 AT2G27110 25.557 583 359 15 127 690 108 634 5.48e-51 191
MS.gene60638.t1 AT2G27110 25.557 583 359 15 127 690 108 634 5.48e-51 191
MS.gene60638.t1 AT2G27110 26.482 506 315 11 187 690 39 489 7.78e-51 189
MS.gene60638.t1 AT1G76320 24.107 672 395 19 50 691 12 598 8.65e-47 176
MS.gene60638.t1 AT1G76320 24.143 671 394 19 50 690 12 597 2.89e-46 176
MS.gene60638.t1 AT1G76320 24.143 671 394 19 50 690 12 597 2.89e-46 176
MS.gene60638.t1 AT1G76320 24.143 671 394 19 50 690 12 597 2.92e-46 176
MS.gene60638.t1 AT5G18960 24.038 624 363 15 24 632 200 727 6.84e-46 175
MS.gene60638.t1 AT5G18960 24.038 624 363 15 24 632 200 727 6.84e-46 175
MS.gene60638.t1 AT3G22170 22.879 660 402 18 34 667 69 647 2.07e-45 174
MS.gene60638.t1 AT3G22170 22.879 660 402 18 34 667 69 647 2.07e-45 174
MS.gene60638.t1 AT4G38170 25.522 431 263 11 206 633 24 399 6.59e-45 169
MS.gene60638.t1 AT2G32250 24.496 694 410 23 1 681 8 600 4.63e-42 164
MS.gene60638.t1 AT2G32250 24.496 694 410 23 1 681 8 600 4.88e-42 164
MS.gene60638.t1 AT2G32250 24.496 694 410 23 1 681 8 600 4.88e-42 164
MS.gene60638.t1 AT2G32250 24.496 694 410 23 1 681 8 600 5.25e-42 164
MS.gene60638.t1 AT2G32250 24.496 694 410 23 1 681 8 600 5.25e-42 164
MS.gene60638.t1 AT2G32250 24.496 694 410 23 1 681 8 600 5.25e-42 164
MS.gene60638.t1 AT2G32250 24.806 645 375 21 50 681 12 559 1.66e-41 162
MS.gene60638.t1 AT2G32250 24.806 645 375 21 50 681 12 559 1.73e-41 162
MS.gene60638.t1 AT1G10240 23.558 624 354 19 49 630 62 604 2.15e-37 149
MS.gene60638.t1 AT1G10240 23.558 624 354 19 49 630 62 604 2.15e-37 149
MS.gene60638.t1 AT5G28530 23.758 644 355 20 40 631 60 619 6.34e-36 144
MS.gene60638.t1 AT1G80010 23.975 634 368 21 41 633 101 661 7.03e-36 145
MS.gene60638.t1 AT5G28530 23.602 644 352 21 40 631 60 615 3.00e-35 142
MS.gene60638.t1 AT5G28530 23.602 644 352 21 40 631 60 615 3.00e-35 142
MS.gene60638.t1 AT1G52520 22.581 620 381 19 41 633 91 638 3.96e-32 133
MS.gene60638.t1 AT4G19990 25.420 417 291 9 41 454 41 440 7.40e-30 126
MS.gene60638.t1 AT4G19990 34.586 133 73 3 562 692 509 629 1.13e-12 72.0
MS.gene60638.t1 AT4G19990 25.420 417 291 9 41 454 27 426 8.14e-30 126
MS.gene60638.t1 AT4G19990 34.586 133 73 3 562 692 495 615 1.01e-12 72.0
MS.gene60638.t1 AT4G19990 25.420 417 291 9 41 454 27 426 9.32e-30 126
MS.gene60638.t1 AT4G19990 34.586 133 73 3 562 692 495 615 1.05e-12 72.0
MS.gene60638.t1 AT4G19990 25.420 417 291 9 41 454 27 426 9.32e-30 126
MS.gene60638.t1 AT4G19990 34.586 133 73 3 562 692 495 615 1.05e-12 72.0
MS.gene60638.t1 AT4G19990 25.420 417 291 9 41 454 27 426 1.17e-29 125
MS.gene60638.t1 AT4G19990 34.586 133 73 3 562 692 495 615 9.76e-13 72.0
MS.gene60638.t1 AT4G19990 25.420 417 291 9 41 454 27 426 1.17e-29 125
MS.gene60638.t1 AT4G19990 34.586 133 73 3 562 692 495 615 9.76e-13 72.0
MS.gene60638.t1 AT4G19990 25.867 375 257 7 83 454 13 369 1.45e-27 119
MS.gene60638.t1 AT4G19990 34.586 133 73 3 562 692 438 558 1.10e-12 72.0
MS.gene60638.t1 AT5G28530 22.259 611 342 21 40 631 60 556 4.13e-23 104

Find 99 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCGATGAGTTAACTAATT+TGG 0.156761 1.2:-69803114 MS.gene60638:CDS
CTTTAGTCATTTCAACATTA+TGG 0.194287 1.2:-69802847 MS.gene60638:CDS
TACATTTCAGCTGCCTTTCT+TGG 0.197151 1.2:-69803395 MS.gene60638:intron
CAACAAGATTATGCTCTATT+TGG 0.198611 1.2:-69803909 MS.gene60638:CDS
CATCGTAAGAGTGTGTTATT+TGG 0.276792 1.2:-69803362 MS.gene60638:CDS
TATGGTTTGAGGTTTCTTAT+TGG 0.280533 1.2:+69803268 None:intergenic
ATAACACTGGATTCTTTGTT+AGG 0.289896 1.2:-69804547 MS.gene60638:CDS
AATCAGATTTCGACTACAAT+TGG 0.318540 1.2:-69803073 MS.gene60638:CDS
TGTTCTTGAGGCAGGGAAAA+AGG 0.322946 1.2:-69802133 MS.gene60638:CDS
ACCAGCCTGGTCTGTGTATA+TGG 0.341000 1.2:+69803250 None:intergenic
GCCGATATTTATTGTTTGTT+CGG 0.341758 1.2:+69803859 None:intergenic
GAGGGATAATCATGCTGATT+TGG 0.346844 1.2:-69802814 MS.gene60638:CDS
GGTTTCGAATGTTGATGTTT+TGG 0.354913 1.2:-69802550 MS.gene60638:CDS
GTCTTTAGTGCCAACATTCA+TGG 0.367086 1.2:+69802257 None:intergenic
AACAATAAATATCGGCCATT+AGG 0.388497 1.2:-69803852 MS.gene60638:intron
TCATGCTGATTTGGACTTCA+AGG 0.389304 1.2:-69802805 MS.gene60638:CDS
ACCGAACAAACAATAAATAT+CGG 0.389395 1.2:-69803860 MS.gene60638:CDS
TTCTCATACTCAACAAGAAC+TGG 0.395633 1.2:+69804389 None:intergenic
TTCCTTCAAATACTATTATG+AGG 0.399583 1.2:-69802673 MS.gene60638:CDS
CCATGATTCTTTATCACTAA+AGG 0.406174 1.2:+69804628 None:intergenic
CTCAACGGTGTTCTTGAGGC+AGG 0.408414 1.2:-69802141 MS.gene60638:CDS
TAAAGAATCATGGAAGTCTT+TGG 0.408500 1.2:-69804618 MS.gene60638:CDS
CATGTCGTATGTTTGAGAAC+CGG 0.411671 1.2:-69802425 MS.gene60638:CDS
GATTATGATCTTCAAACCTT+GGG 0.413114 1.2:+69802502 None:intergenic
GGGTTGTCACAGTCGACATT+AGG 0.419786 1.2:-69802467 MS.gene60638:CDS
CGATTATGATCTTCAAACCT+TGG 0.425681 1.2:+69802501 None:intergenic
CGAGAAGTAAATGAAGTTCA+AGG 0.430014 1.2:-69804212 MS.gene60638:CDS
AGCTGGAATATATTTGAAGT+TGG 0.433136 1.2:-69804330 MS.gene60638:CDS
TGAATGTCTATAAGATATAT+AGG 0.445219 1.2:-69804298 MS.gene60638:CDS
AGAAGTATTTCAGATGTCTT+CGG 0.445323 1.2:+69802162 None:intergenic
TGGTCTGTGTATATGGTTTG+AGG 0.447350 1.2:+69803257 None:intergenic
CAACTCACAAAATAAGAAAT+CGG 0.447367 1.2:-69804504 MS.gene60638:CDS
GAAAGGGTTGGTTGTAAAGC+TGG 0.447509 1.2:-69804347 MS.gene60638:CDS
CGCTTTAAAACATATTGTGC+AGG 0.447633 1.2:+69802324 None:intergenic
CGTTATGCTTGTAACAAAGA+AGG 0.449439 1.2:-69804464 MS.gene60638:CDS
GCTTTAATAAATTCTAAAGC+TGG 0.450264 1.2:-69804182 MS.gene60638:CDS
TATGCATTGGTCTTCAGCTT+GGG 0.450920 1.2:-69802973 MS.gene60638:CDS
ATCTGAGAGTGGACAATTGA+CGG 0.452529 1.2:-69804693 MS.gene60638:CDS
TGGTCTTTGCTCATGGCACA+TGG 0.452802 1.2:-69803183 MS.gene60638:CDS
GGCCTACTTCGGACTTTAAG+TGG 0.458978 1.2:-69803019 MS.gene60638:CDS
ATGTCGTATGTTTGAGAACC+GGG 0.461344 1.2:-69802424 MS.gene60638:CDS
CGCAAACACGTGTAGAAAGC+TGG 0.465682 1.2:+69802190 None:intergenic
GTATGCATTGGTCTTCAGCT+TGG 0.479582 1.2:-69802974 MS.gene60638:CDS
TTCATTCAAATCGTATGCAT+TGG 0.479750 1.2:-69802986 MS.gene60638:CDS
CTCCTCATAATAGTATTTGA+AGG 0.485089 1.2:+69802671 None:intergenic
TTACAACCAACCCTTTCCTC+TGG 0.485330 1.2:+69804353 None:intergenic
TTTAATTATGCATATAACAC+TGG 0.485542 1.2:-69804560 MS.gene60638:CDS
ACATTATGGTTCAAAGAATG+AGG 0.490425 1.2:-69802833 MS.gene60638:CDS
TATGAGGAGGACACAACAAA+AGG 0.490908 1.2:-69802657 MS.gene60638:CDS
ATGATTGTCAAAACCAAGAA+AGG 0.491378 1.2:+69803382 None:intergenic
ATCAGATTTCGACTACAATT+GGG 0.491708 1.2:-69803072 MS.gene60638:CDS
ACAAAATAAGAAATCGGATA+AGG 0.492141 1.2:-69804498 MS.gene60638:CDS
GACCGCATAAGCTGACTGTT+GGG 0.496307 1.2:+69802753 None:intergenic
TGTCTTCTGCAACATCAACT+TGG 0.500580 1.2:+69803964 None:intergenic
ATTGGTCTTCAGCTTGGGTG+AGG 0.501327 1.2:-69802968 MS.gene60638:CDS
CATCAAAAGGAGATGGTGGC+TGG 0.501610 1.2:-69804056 MS.gene60638:intron
ATCATAATCGACTTGATGAA+AGG 0.507955 1.2:-69802488 MS.gene60638:CDS
GATCTTCAAACCTTGGGGAA+AGG 0.519004 1.2:+69802508 None:intergenic
TAAAGAATCGAACAAGTGAA+TGG 0.519618 1.2:+69802865 None:intergenic
TGTCTATAAGATATATAGGT+GGG 0.523740 1.2:-69804294 MS.gene60638:CDS
TCATAATCGACTTGATGAAA+GGG 0.526860 1.2:-69802487 MS.gene60638:CDS
CATAATAGTATTTGAAGGAA+AGG 0.529686 1.2:+69802676 None:intergenic
TCATGGCACATGGGAGAGAA+TGG 0.532310 1.2:-69803173 MS.gene60638:CDS
CTGAAATCTACCATGAATGT+TGG 0.534154 1.2:-69802267 MS.gene60638:CDS
TAACAAAGAAGGTTTCAAGA+AGG 0.538422 1.2:-69804453 MS.gene60638:CDS
AATCGAACAAGTGAATGGTC+TGG 0.540971 1.2:+69802870 None:intergenic
ATGAGGAGGACACAACAAAA+GGG 0.542065 1.2:-69802656 MS.gene60638:CDS
CCTTTAGTGATAAAGAATCA+TGG 0.543522 1.2:-69804628 MS.gene60638:CDS
AACAAAGAAGGTTTCAAGAA+GGG 0.545537 1.2:-69804452 MS.gene60638:CDS
ACTTCAACAACGCACCAGCC+TGG 0.557139 1.2:+69803237 None:intergenic
AATGAAGCTCTCTCGAAACC+AGG 0.565261 1.2:-69804002 MS.gene60638:CDS
TCAACGGTGTTCTTGAGGCA+GGG 0.570070 1.2:-69802140 MS.gene60638:CDS
ACTTCAAGGCTGCAAACACT+AGG 0.573822 1.2:-69802791 MS.gene60638:CDS
ACTTCTCAACGGTGTTCTTG+AGG 0.574595 1.2:-69802145 MS.gene60638:CDS
GGACCGCATAAGCTGACTGT+TGG 0.579264 1.2:+69802752 None:intergenic
TTGAAGAAAACCTTTCCCCA+AGG 0.580876 1.2:-69802518 MS.gene60638:CDS
AAATGCTCATGTTTATGTAA+TGG 0.581422 1.2:+69804254 None:intergenic
GAGAGTGGACAATTGACGGT+TGG 0.584622 1.2:-69804689 MS.gene60638:CDS
TGGTGTATACTTGTTTACTA+CGG 0.585960 1.2:+69802731 None:intergenic
ACCATATACACAGACCAGGC+TGG 0.590176 1.2:-69803251 MS.gene60638:CDS
AACTCATCAAAAGGAGATGG+TGG 0.593854 1.2:-69804060 MS.gene60638:intron
TTGATATCGTTGTGCTTGAG+TGG 0.601464 1.2:+69802233 None:intergenic
ATCCCAACAGTCAGCTTATG+CGG 0.606153 1.2:-69802755 MS.gene60638:CDS
CTTCAAATACTATTATGAGG+AGG 0.607664 1.2:-69802670 MS.gene60638:CDS
CATTATGGTTCAAAGAATGA+GGG 0.609190 1.2:-69802832 MS.gene60638:CDS
ATATCAACTTCATCTGAGAG+TGG 0.614980 1.2:-69804704 MS.gene60638:CDS
AGAACTGGACTGTCACATGT+TGG 0.620791 1.2:+69804404 None:intergenic
AGCCACTTAAAGTCCGAAGT+AGG 0.621799 1.2:+69803017 None:intergenic
ATTATGATCTTCAAACCTTG+GGG 0.630358 1.2:+69802503 None:intergenic
GGTCTTTGCTCATGGCACAT+GGG 0.639768 1.2:-69803182 MS.gene60638:CDS
ATGTCTATAAGATATATAGG+TGG 0.643489 1.2:-69804295 MS.gene60638:CDS
GATTTCAGCTTATCGACAGA+CGG 0.644926 1.2:+69802282 None:intergenic
ACATCTGAAATACTTCTCAA+CGG 0.652966 1.2:-69802156 MS.gene60638:CDS
TGAATAAATGAAAAGCAAGA+TGG 0.655697 1.2:+69802711 None:intergenic
CATGTCGACATAAGAAACCC+CGG 0.663279 1.2:+69802406 None:intergenic
TCAAACCATATACACAGACC+AGG 0.671057 1.2:-69803255 MS.gene60638:CDS
TGTCGTATGTTTGAGAACCG+GGG 0.673760 1.2:-69802423 MS.gene60638:CDS
ATGTTGATAATGATAATGAG+AGG 0.683310 1.2:-69802572 MS.gene60638:CDS
TGTTGATAATGATAATGAGA+GGG 0.689129 1.2:-69802571 MS.gene60638:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAATGTCTATAAGATATAT+AGG - chr1.2:69802540-69802559 MS.gene60638:CDS 20.0%
!! TTTAATTATGCATATAACAC+TGG - chr1.2:69802278-69802297 MS.gene60638:CDS 20.0%
!!! ATGATGAAAAACTGTTTTAA+TGG - chr1.2:69803794-69803813 MS.gene60638:intron 20.0%
!!! TGAATGTTTATATTGATTGA+AGG - chr1.2:69803133-69803152 MS.gene60638:CDS 20.0%
! AAATGCTCATGTTTATGTAA+TGG + chr1.2:69802587-69802606 None:intergenic 25.0%
! ACAAAATAAGAAATCGGATA+AGG - chr1.2:69802340-69802359 MS.gene60638:CDS 25.0%
! ACCGAACAAACAATAAATAT+CGG - chr1.2:69802978-69802997 MS.gene60638:CDS 25.0%
! AGTACAAAATACTAACCTAA+TGG + chr1.2:69803004-69803023 None:intergenic 25.0%
! ATGTCTATAAGATATATAGG+TGG - chr1.2:69802543-69802562 MS.gene60638:CDS 25.0%
! CAACTCACAAAATAAGAAAT+CGG - chr1.2:69802334-69802353 MS.gene60638:CDS 25.0%
! CATAATAGTATTTGAAGGAA+AGG + chr1.2:69804165-69804184 None:intergenic 25.0%
! CTTTAGTCATTTCAACATTA+TGG - chr1.2:69803991-69804010 MS.gene60638:CDS 25.0%
! TGAAATTTGAATGAATGTTC+TGG - chr1.2:69803051-69803070 MS.gene60638:CDS 25.0%
! TGAATAAATGAAAAGCAAGA+TGG + chr1.2:69804130-69804149 None:intergenic 25.0%
! TGTCTATAAGATATATAGGT+GGG - chr1.2:69802544-69802563 MS.gene60638:CDS 25.0%
! TTCCTTCAAATACTATTATG+AGG - chr1.2:69804165-69804184 MS.gene60638:CDS 25.0%
! TTTCGATGAGTTAACTAATT+TGG - chr1.2:69803724-69803743 MS.gene60638:intron 25.0%
! TTTCTATTTACACAACACAA+TGG - chr1.2:69803372-69803391 MS.gene60638:CDS 25.0%
!! AGGTTTAGAATTTAATGTTC+TGG - chr1.2:69803153-69803172 MS.gene60638:CDS 25.0%
!! TCAATTTCGAATGTTTTTCA+AGG - chr1.2:69803623-69803642 MS.gene60638:intron 25.0%
!! TGTTGTATTTTGTTCTGATT+TGG - chr1.2:69803414-69803433 MS.gene60638:intron 25.0%
!!! AAGACATAGCTAGTATTTTT+TGG - chr1.2:69802891-69802910 MS.gene60638:CDS 25.0%
!!! AGACATAGCTAGTATTTTTT+GGG - chr1.2:69802892-69802911 MS.gene60638:CDS 25.0%
!!! ATGTTGATAATGATAATGAG+AGG - chr1.2:69804266-69804285 MS.gene60638:CDS 25.0%
!!! GCTTTAATAAATTCTAAAGC+TGG - chr1.2:69802656-69802675 MS.gene60638:CDS 25.0%
!!! TGAAAAACTGTTTTAATGGA+AGG - chr1.2:69803798-69803817 MS.gene60638:intron 25.0%
!!! TGTTGATAATGATAATGAGA+GGG - chr1.2:69804267-69804286 MS.gene60638:CDS 25.0%
AAACACATATGATCTTTCCA+TGG + chr1.2:69803224-69803243 None:intergenic 30.0%
AACAAAGAAGGTTTCAAGAA+GGG - chr1.2:69802386-69802405 MS.gene60638:CDS 30.0%
AACAATAAATATCGGCCATT+AGG - chr1.2:69802986-69803005 MS.gene60638:CDS 30.0%
AATCAGATTTCGACTACAAT+TGG - chr1.2:69803765-69803784 MS.gene60638:intron 30.0%
ACATTATGGTTCAAAGAATG+AGG - chr1.2:69804005-69804024 MS.gene60638:CDS 30.0%
AGAACATCTTATGAAGTGAT+GGG - chr1.2:69802689-69802708 MS.gene60638:CDS 30.0%
ATAACACTGGATTCTTTGTT+AGG - chr1.2:69802291-69802310 MS.gene60638:CDS 30.0%
ATCAGATTTCGACTACAATT+GGG - chr1.2:69803766-69803785 MS.gene60638:intron 30.0%
ATGATTGTCAAAACCAAGAA+AGG + chr1.2:69803459-69803478 None:intergenic 30.0%
ATTATGATCTTCAAACCTTG+GGG + chr1.2:69804338-69804357 None:intergenic 30.0%
CAACAAGATTATGCTCTATT+TGG - chr1.2:69802929-69802948 MS.gene60638:CDS 30.0%
CATTATGGTTCAAAGAATGA+GGG - chr1.2:69804006-69804025 MS.gene60638:CDS 30.0%
CCATGATTCTTTATCACTAA+AGG + chr1.2:69802213-69802232 None:intergenic 30.0%
CCTTTAGTGATAAAGAATCA+TGG - chr1.2:69802210-69802229 MS.gene60638:CDS 30.0%
CTCCTCATAATAGTATTTGA+AGG + chr1.2:69804170-69804189 None:intergenic 30.0%
CTTCAAATACTATTATGAGG+AGG - chr1.2:69804168-69804187 MS.gene60638:CDS 30.0%
GAGAATGGAAAGAAAAATCT+CGG - chr1.2:69803680-69803699 MS.gene60638:intron 30.0%
GATTATGATCTTCAAACCTT+GGG + chr1.2:69804339-69804358 None:intergenic 30.0%
GCCGATATTTATTGTTTGTT+CGG + chr1.2:69802982-69803001 None:intergenic 30.0%
TAAAGAAAAACCAGAGGAAA+GGG - chr1.2:69802475-69802494 MS.gene60638:CDS 30.0%
TAAAGAATCATGGAAGTCTT+TGG - chr1.2:69802220-69802239 MS.gene60638:CDS 30.0%
TAAAGAATCGAACAAGTGAA+TGG + chr1.2:69803976-69803995 None:intergenic 30.0%
TAACAAAGAAGGTTTCAAGA+AGG - chr1.2:69802385-69802404 MS.gene60638:CDS 30.0%
TCTAATGACAACTCATCAAA+AGG - chr1.2:69802769-69802788 MS.gene60638:CDS 30.0%
TGGATATCATAGTAAAAACC+TGG + chr1.2:69802857-69802876 None:intergenic 30.0%
TGGTGTATACTTGTTTACTA+CGG + chr1.2:69804110-69804129 None:intergenic 30.0%
TTCATTCAAATCGTATGCAT+TGG - chr1.2:69803852-69803871 MS.gene60638:intron 30.0%
TTTAAGTCCGAAAATCGAAA+AGG + chr1.2:69802743-69802762 None:intergenic 30.0%
! ACATCTGAAATACTTCTCAA+CGG - chr1.2:69804682-69804701 MS.gene60638:CDS 30.0%
! AGCTGGAATATATTTGAAGT+TGG - chr1.2:69802508-69802527 MS.gene60638:CDS 30.0%
! CAAAGTTTTCACAATTGAGA+AGG - chr1.2:69804225-69804244 MS.gene60638:CDS 30.0%
! GAATCTTCCTTTTCGATTTT+CGG - chr1.2:69802733-69802752 MS.gene60638:CDS 30.0%
! TATGGTTTGAGGTTTCTTAT+TGG + chr1.2:69803573-69803592 None:intergenic 30.0%
!! ACATGTTTTCAAGATTTTGG+AGG - chr1.2:69804450-69804469 MS.gene60638:CDS 30.0%
!! AGAAGTATTTCAGATGTCTT+CGG + chr1.2:69804679-69804698 None:intergenic 30.0%
!! ATCATAATCGACTTGATGAA+AGG - chr1.2:69804350-69804369 MS.gene60638:CDS 30.0%
!! ATGTTCTGGTTTAGAATTGA+AGG - chr1.2:69803065-69803084 MS.gene60638:CDS 30.0%
!! TCATAATCGACTTGATGAAA+GGG - chr1.2:69804351-69804370 MS.gene60638:CDS 30.0%
!! TCGACATGTTTTCAAGATTT+TGG - chr1.2:69804447-69804466 MS.gene60638:CDS 30.0%
!!! CTGCACAATATGTTTTAAAG+CGG - chr1.2:69804515-69804534 MS.gene60638:CDS 30.0%
!!! CTTATTTTGTGAGTTGTTCT+TGG + chr1.2:69802330-69802349 None:intergenic 30.0%
!!! GTTTTTCAAGGTGTTTTTCA+TGG - chr1.2:69803635-69803654 MS.gene60638:intron 30.0%
ATATCAACTTCATCTGAGAG+TGG - chr1.2:69802134-69802153 MS.gene60638:CDS 35.0%
CGAGAAGTAAATGAAGTTCA+AGG - chr1.2:69802626-69802645 MS.gene60638:CDS 35.0%
CGATTATGATCTTCAAACCT+TGG + chr1.2:69804340-69804359 None:intergenic 35.0%
CGTTATGCTTGTAACAAAGA+AGG - chr1.2:69802374-69802393 MS.gene60638:CDS 35.0%
CTAAAGAAAAACCAGAGGAA+AGG - chr1.2:69802474-69802493 MS.gene60638:CDS 35.0%
CTGAAATCTACCATGAATGT+TGG - chr1.2:69804571-69804590 MS.gene60638:CDS 35.0%
CTGGTATATGCGTGTTAATT+TGG - chr1.2:69803090-69803109 MS.gene60638:CDS 35.0%
GAAAGCTAAAGAAAAACCAG+AGG - chr1.2:69802469-69802488 MS.gene60638:CDS 35.0%
GAGAACATCTTATGAAGTGA+TGG - chr1.2:69802688-69802707 MS.gene60638:CDS 35.0%
GTCTGCTTAATCCTAAAAAC+AGG - chr1.2:69802411-69802430 MS.gene60638:CDS 35.0%
TGTACAGAGCACAAATTCTA+AGG + chr1.2:69803200-69803219 None:intergenic 35.0%
TTCTCATACTCAACAAGAAC+TGG + chr1.2:69802452-69802471 None:intergenic 35.0%
! CATCGTAAGAGTGTGTTATT+TGG - chr1.2:69803476-69803495 MS.gene60638:intron 35.0%
! GGTTTCGAATGTTGATGTTT+TGG - chr1.2:69804288-69804307 MS.gene60638:CDS 35.0%
! TCTTTCAATGCTTTTCTTCG+TGG - chr1.2:69803935-69803954 MS.gene60638:CDS 35.0%
! TGGTTTAGAATTGAAGGTTC+TGG - chr1.2:69803071-69803090 MS.gene60638:CDS 35.0%
! TTTTAATGGAAGGCCTACTT+CGG - chr1.2:69803808-69803827 MS.gene60638:intron 35.0%
! TTTTTCATGGTCTTTGCTCA+TGG - chr1.2:69803648-69803667 MS.gene60638:intron 35.0%
!! CGCTTTAAAACATATTGTGC+AGG + chr1.2:69804517-69804536 None:intergenic 35.0%
!! TCTTATGAAGTGATGGGTTA+AGG - chr1.2:69802695-69802714 MS.gene60638:CDS 35.0%
!! TTCAAGATTTTGGAGGTCTT+AGG - chr1.2:69804457-69804476 MS.gene60638:CDS 35.0%
!!! AGACATCAGCTACTTTTGAT+TGG - chr1.2:69803519-69803538 MS.gene60638:intron 35.0%
!!! CACAATATGTTTTAAAGCGG+TGG - chr1.2:69804518-69804537 MS.gene60638:CDS 35.0%
AACTCATCAAAAGGAGATGG+TGG - chr1.2:69802778-69802797 MS.gene60638:CDS 40.0%
AATCGAACAAGTGAATGGTC+TGG + chr1.2:69803971-69803990 None:intergenic 40.0%
ACCTATGATCTTTGCATAGC+TGG + chr1.2:69803322-69803341 None:intergenic 40.0%
ATCTGAGAGTGGACAATTGA+CGG - chr1.2:69802145-69802164 MS.gene60638:CDS 40.0%
ATGAGGAGGACACAACAAAA+GGG - chr1.2:69804182-69804201 MS.gene60638:CDS 40.0%
ATGTCGTATGTTTGAGAACC+GGG - chr1.2:69804414-69804433 MS.gene60638:CDS 40.0%
CATGTCGTATGTTTGAGAAC+CGG - chr1.2:69804413-69804432 MS.gene60638:CDS 40.0%
GACAACTCATCAAAAGGAGA+TGG - chr1.2:69802775-69802794 MS.gene60638:CDS 40.0%
GATTTCAGCTTATCGACAGA+CGG + chr1.2:69804559-69804578 None:intergenic 40.0%
GTCTTTAGTGCCAACATTCA+TGG + chr1.2:69804584-69804603 None:intergenic 40.0%
TACATTTCAGCTGCCTTTCT+TGG - chr1.2:69803443-69803462 MS.gene60638:intron 40.0%
TATGAGGAGGACACAACAAA+AGG - chr1.2:69804181-69804200 MS.gene60638:CDS 40.0%
TCAAACCATATACACAGACC+AGG - chr1.2:69803583-69803602 MS.gene60638:intron 40.0%
TCCAGCTATGCAAAGATCAT+AGG - chr1.2:69803318-69803337 MS.gene60638:CDS 40.0%
TGGTCTGTGTATATGGTTTG+AGG + chr1.2:69803584-69803603 None:intergenic 40.0%
TGTCTTCTGCAACATCAACT+TGG + chr1.2:69802877-69802896 None:intergenic 40.0%
TTGAAGAAAACCTTTCCCCA+AGG - chr1.2:69804320-69804339 MS.gene60638:CDS 40.0%
TTGATATCGTTGTGCTTGAG+TGG + chr1.2:69804608-69804627 None:intergenic 40.0%
! GAGGGATAATCATGCTGATT+TGG - chr1.2:69804024-69804043 MS.gene60638:CDS 40.0%
! GTCACATTTTACAGCTGGTT+TGG - chr1.2:69803892-69803911 MS.gene60638:CDS 40.0%
!! AAGATTTTGGAGGTCTTAGG+TGG - chr1.2:69804460-69804479 MS.gene60638:CDS 40.0%
!! TATGCATTGGTCTTCAGCTT+GGG - chr1.2:69803865-69803884 MS.gene60638:CDS 40.0%
!! TCACATGTTGGCCTGTTTTT+AGG + chr1.2:69802425-69802444 None:intergenic 40.0%
!! TCATGCTGATTTGGACTTCA+AGG - chr1.2:69804033-69804052 MS.gene60638:CDS 40.0%
!!! GCTAGTATTTTTTGGGCAGA+TGG - chr1.2:69802899-69802918 MS.gene60638:CDS 40.0%
AATGAAGCTCTCTCGAAACC+AGG - chr1.2:69802836-69802855 MS.gene60638:CDS 45.0%
ACTTCAAGGCTGCAAACACT+AGG - chr1.2:69804047-69804066 MS.gene60638:CDS 45.0%
ACTTCTCAACGGTGTTCTTG+AGG - chr1.2:69804693-69804712 MS.gene60638:CDS 45.0%
AGAACTGGACTGTCACATGT+TGG + chr1.2:69802437-69802456 None:intergenic 45.0%
AGCCACTTAAAGTCCGAAGT+AGG + chr1.2:69803824-69803843 None:intergenic 45.0%
ATCCCAACAGTCAGCTTATG+CGG - chr1.2:69804083-69804102 MS.gene60638:intron 45.0%
ATTTGTGCTCTGTACAGCCA+TGG - chr1.2:69803204-69803223 MS.gene60638:CDS 45.0%
CATGTCGACATAAGAAACCC+CGG + chr1.2:69804435-69804454 None:intergenic 45.0%
GAAAAACCAGAGGAAAGGGT+TGG - chr1.2:69802479-69802498 MS.gene60638:CDS 45.0%
GATCTTCAAACCTTGGGGAA+AGG + chr1.2:69804333-69804352 None:intergenic 45.0%
TGTCGTATGTTTGAGAACCG+GGG - chr1.2:69804415-69804434 MS.gene60638:CDS 45.0%
TGTTCTTGAGGCAGGGAAAA+AGG - chr1.2:69804705-69804724 MS.gene60638:CDS 45.0%
TTACAACCAACCCTTTCCTC+TGG + chr1.2:69802488-69802507 None:intergenic 45.0%
! GTGAGGTCACATTTTACAGC+TGG - chr1.2:69803887-69803906 MS.gene60638:CDS 45.0%
!! CAGAGCACAAATTCTAAGGC+AGG + chr1.2:69803196-69803215 None:intergenic 45.0%
!! GAAAGGGTTGGTTGTAAAGC+TGG - chr1.2:69802491-69802510 MS.gene60638:CDS 45.0%
!! GAAGTGATGGGTTAAGGAGT+TGG - chr1.2:69802701-69802720 MS.gene60638:CDS 45.0%
!! GTATGCATTGGTCTTCAGCT+TGG - chr1.2:69803864-69803883 MS.gene60638:CDS 45.0%
ACCAGCCTGGTCTGTGTATA+TGG + chr1.2:69803591-69803610 None:intergenic 50.0%
ACCATATACACAGACCAGGC+TGG - chr1.2:69803587-69803606 MS.gene60638:intron 50.0%
CGCAAACACGTGTAGAAAGC+TGG + chr1.2:69804651-69804670 None:intergenic 50.0%
GACCGCATAAGCTGACTGTT+GGG + chr1.2:69804088-69804107 None:intergenic 50.0%
GAGAGTGGACAATTGACGGT+TGG - chr1.2:69802149-69802168 MS.gene60638:CDS 50.0%
GGCCTACTTCGGACTTTAAG+TGG - chr1.2:69803819-69803838 MS.gene60638:intron 50.0%
GGGTTGTCACAGTCGACATT+AGG - chr1.2:69804371-69804390 MS.gene60638:CDS 50.0%
GGTCTTTGCTCATGGCACAT+GGG - chr1.2:69803656-69803675 MS.gene60638:intron 50.0%
TCAACGGTGTTCTTGAGGCA+GGG - chr1.2:69804698-69804717 MS.gene60638:CDS 50.0%
TCATGGCACATGGGAGAGAA+TGG - chr1.2:69803665-69803684 MS.gene60638:intron 50.0%
TGGTCTTTGCTCATGGCACA+TGG - chr1.2:69803655-69803674 MS.gene60638:intron 50.0%
! CATCAAAAGGAGATGGTGGC+TGG - chr1.2:69802782-69802801 MS.gene60638:CDS 50.0%
!! ATTGGTCTTCAGCTTGGGTG+AGG - chr1.2:69803870-69803889 MS.gene60638:CDS 50.0%
!! GTGATGGGTTAAGGAGTTGG+AGG - chr1.2:69802704-69802723 MS.gene60638:CDS 50.0%
ACTTCAACAACGCACCAGCC+TGG + chr1.2:69803604-69803623 None:intergenic 55.0%
CTCAACGGTGTTCTTGAGGC+AGG - chr1.2:69804697-69804716 MS.gene60638:CDS 55.0%
GGACCGCATAAGCTGACTGT+TGG + chr1.2:69804089-69804108 None:intergenic 55.0%
GGGTTAAGGAGTTGGAGGCA+CGG - chr1.2:69802709-69802728 MS.gene60638:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 69802125 69804735 69802125 ID=MS.gene60638
chr1.2 mRNA 69802125 69804735 69802125 ID=MS.gene60638.t1;Parent=MS.gene60638
chr1.2 exon 69804157 69804735 69804157 ID=MS.gene60638.t1.exon1;Parent=MS.gene60638.t1
chr1.2 CDS 69804157 69804735 69804157 ID=cds.MS.gene60638.t1;Parent=MS.gene60638.t1
chr1.2 exon 69803853 69804069 69803853 ID=MS.gene60638.t1.exon2;Parent=MS.gene60638.t1
chr1.2 CDS 69803853 69804069 69803853 ID=cds.MS.gene60638.t1;Parent=MS.gene60638.t1
chr1.2 exon 69802125 69803407 69802125 ID=MS.gene60638.t1.exon3;Parent=MS.gene60638.t1
chr1.2 CDS 69802125 69803407 69802125 ID=cds.MS.gene60638.t1;Parent=MS.gene60638.t1
Gene Sequence

>MS.gene60638

ATGGATTTGATATCAACTTCATCTGAGAGTGGACAATTGACGGTTGGAAATTCAGTTATTGATGCAGATAGTTCTCAATGTTACTCCTTTAGTGATAAAGAATCATGGAAGTCTTTGGTCTTTAAGTCTATAAAAGAGATTGAAGATTTTTATTTTAATTATGCATATAACACTGGATTCTTTGTTAGGAGTATGTTGAAGTCCAAGAACAACTCACAAAATAAGAAATCGGATAAGGTACATTATGTGCGTTATGCTTGTAACAAAGAAGGTTTCAAGAAGGGAAGTCTGCTTAATCCTAAAAACAGGCCAACATGTGACAGTCCAGTTCTTGTTGAGTATGAGAAAGCTAAAGAAAAACCAGAGGAAAGGGTTGGTTGTAAAGCTGGAATATATTTGAAGTTGGATGAAGCGTTGAATGTCTATAAGATATATAGGTGGGAAGTTGATCACTGTCATCCATTACATAAACATGAGCATTTGCGCTACTTGAGATCATTTCGAGAAGTAAATGAAGTTCAAGGACAACTTGCTTTAATAAATTCTAAAGCTGGAATGTCGATGAGAACATCTTATGAAGTGATGGGTTAAGGAGTTGGAGGCACGGAGAATCTTCCTTTTCGATTTTCGGACTTAAAGAACTATCTAATGACAACTCATCAAAAGGAGATGGTGGCTGGTGAAGCAATTGTTATTCAAGAATTCTTTAGAAATGAAGCTCTCTCGAAACCAGGTTTTTACTATGATATCCAAGTTGATGTTGCAGAAGACATAGCTAGTATTTTTTGGGCAGATGGAATTATGCAACAAGATTATGCTCTATTTGGTGATGTTGTCAGTTTTGATACAACTTACCGAACAAACAATAAATATCGGCCATTAGGTTAGTATTTTGTACTTCTCACAGTTTTTAATTCTTATATTCTTGAAATTTGAATGAATGTTCTGGTTTAGAATTGAAGGTTCTGGTATATGCGTGTTAATTTGGCTCTTGTGTGTGTTTAGAATTGAATGTTTATATTGATTGAAGGTTTAGAATTTAATGTTCTGGTTTATGAGTGATCTTATCCTGCCTTAGAATTTGTGCTCTGTACAGCCATGGAAAGATCATATGTGTTTTTTTATCGATGATAAATAAATAGCTCTTTCTGTGTTTGCGAGTTTAGACATCTCGCTTGAGAAGTTGTGCTATGTCCAGCTATGCAAAGATCATAGGTGTTTTTTTACAGTTGTTACATGAATAGTTTTTTCTATTTACACAACACAATGGTTTTATATTTTTTTAAATATGTTGTATTTTGTTCTGATTTGGTTCATTTACATTTCAGCTGCCTTTCTTGGTTTTGACAATCATCGTAAGAGTGTGTTATTTGGTGCTGCGCTACTGTATGATGAGACATCAGCTACTTTTGATTGGTTGTTTACAACTTTTTTGAAGTGTATGTCCAATAAGAAACCTCAAACCATATACACAGACCAGGCTGGTGCGTTGTTGAAGTCAATTTCGAATGTTTTTCAAGGTGTTTTTCATGGTCTTTGCTCATGGCACATGGGAGAGAATGGAAAGAAAAATCTCGGCTCTCGTGCAAACAGTGCATTTTTCGATGAGTTAACTAATTTGGTTTCAAATGTCGAAGATGAATCAGATTTCGACTACAATTGGGATCAGATGATGAAAAACTGTTTTAATGGAAGGCCTACTTCGGACTTTAAGTGGCTTGTTCAAATTCATTCAAATCGTATGCATTGGTCTTCAGCTTGGGTGAGGTCACATTTTACAGCTGGTTTGGAAACGACTCAGTTAAGTGAGTCTTTCAATGCTTTTCTTCGTGGATTTTTGCAGCCAGACCATTCACTTGTTCGATTCTTTAGTCATTTCAACATTATGGTTCAAAGAATGAGGGATAATCATGCTGATTTGGACTTCAAGGCTGCAAACACTAGGACAAAAAATAATTATCCCAACAGTCAGCTTATGCGGTCCGTAGTAAACAAGTATACACCATCTTGCTTTTCATTTATTCACAGTCAGTATGACCTTTCCTTCAAATACTATTATGAGGAGGACACAACAAAAGGGTCTGAATTGAACAAGTTCTTCAAAGTTTTCACAATTGAGAAGGTTGATTATAATTACGATGATGTTGATAATGATAATGAGAGGGTTTCGAATGTTGATGTTTTGGATGCAGAACTTGAAGAAAACCTTTCCCCAAGGTTTGAAGATCATAATCGACTTGATGAAAGGGTTGTCACAGTCGACATTAGGAGCAAAAGTTTTACTTGTTCATGTCGTATGTTTGAGAACCGGGGTTTCTTATGTCGACATGTTTTCAAGATTTTGGAGGTCTTAGGTGGTCATGTGCAATATCATTTTTTGAAGACTATACCTGCACAATATGTTTTAAAGCGGTGGACTAGAGATGTGCGTCCGTCTGTCGATAAGCTGAAATCTACCATGAATGTTGGCACTAAAGACACCACTCAAGCACAACGATATCAACAAATTTGTGCTGTTACAGTCCAGCTTTCTACACGTGTTTGCGCAGATCCGAAGACATCTGAAATACTTCTCAACGGTGTTCTTGAGGCAGGGAAAAAGGCAGAATAG
Protein sequence

>MS.gene60638.t1

MDLISTSSESGQLTVGNSVIDADSSQCYSFSDKESWKSLVFKSIKEIEDFYFNYAYNTGFFVRSMLKSKNNSQNKKSDKVHYVRYACNKEGFKKGSLLNPKNRPTCDSPVLVEYEKAKEKPEERVGCKAGIYLKLDEALNVYKIYRWEVDHCHPLHKHEHLRYLRSFREVNEVQGQLALINSKAGMSMRTSYEEMVAGEAIVIQEFFRNEALSKPGFYYDIQVDVAEDIASIFWADGIMQQDYALFGDVVSFDTTYRTNNKYRPLAAFLGFDNHRKSVLFGAALLYDETSATFDWLFTTFLKCMSNKKPQTIYTDQAGALLKSISNVFQGVFHGLCSWHMGENGKKNLGSRANSAFFDELTNLVSNVEDESDFDYNWDQMMKNCFNGRPTSDFKWLVQIHSNRMHWSSAWVRSHFTAGLETTQLSESFNAFLRGFLQPDHSLVRFFSHFNIMVQRMRDNHADLDFKAANTRTKNNYPNSQLMRSVVNKYTPSCFSFIHSQYDLSFKYYYEEDTTKGSELNKFFKVFTIEKVDYNYDDVDNDNERVSNVDVLDAELEENLSPRFEDHNRLDERVVTVDIRSKSFTCSCRMFENRGFLCRHVFKILEVLGGHVQYHFLKTIPAQYVLKRWTRDVRPSVDKLKSTMNVGTKDTTQAQRYQQICAVTVQLSTRVCADPKTSEILLNGVLEAGKKAE