AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60640.t1 MTR_6g053640 55.645 124 53 2 448 569 2 125 5.81e-34 125
MS.gene60640.t1 MTR_4g035820 27.838 370 231 7 54 409 75 422 6.54e-32 131
MS.gene60640.t1 MTR_3g005220 59.406 101 37 2 8 107 28 125 2.09e-31 119
MS.gene60640.t1 MTR_6g025550 46.763 139 33 3 241 372 23 127 4.48e-31 117
MS.gene60640.t1 MTR_6g024175 65.854 82 27 1 469 549 103 184 3.82e-26 105
MS.gene60640.t1 MTR_2g098880 44.954 109 59 1 52 159 59 167 1.02e-24 103
MS.gene60640.t1 MTR_2g098880 44.954 109 59 1 52 159 71 179 1.58e-24 103
MS.gene60640.t1 MTR_0558s0020 29.362 235 142 6 53 265 35 267 6.94e-22 96.7
MS.gene60640.t1 MTR_1g070355 27.962 211 145 4 59 265 79 286 1.40e-21 99.0
MS.gene60640.t1 MTR_1g083640 29.487 234 141 6 53 264 50 281 1.58e-21 95.5
MS.gene60640.t1 MTR_6g051425 27.876 226 150 6 49 265 12 233 1.60e-20 93.6
MS.gene60640.t1 MTR_2g098920 38.596 114 58 3 53 161 79 185 7.95e-19 87.0
MS.gene60640.t1 MTR_4g067290 33.582 134 77 3 28 156 30 156 3.41e-18 84.3
MS.gene60640.t1 MTR_4g067290 33.582 134 77 3 28 156 30 156 3.58e-18 84.3
MS.gene60640.t1 MTR_4g067290 33.582 134 77 3 28 156 48 174 4.55e-18 84.7
MS.gene60640.t1 MTR_4g067290 33.582 134 77 3 28 156 50 176 4.76e-18 84.7
MS.gene60640.t1 MTR_4g067290 33.582 134 77 3 28 156 50 176 4.79e-18 84.7
MS.gene60640.t1 MTR_4g067290 33.582 134 77 3 28 156 90 216 6.54e-18 85.1
MS.gene60640.t1 MTR_1g008290 34.921 126 77 3 127 250 22 144 4.81e-17 78.6
MS.gene60640.t1 MTR_1g013060 28.507 221 139 6 46 254 16 229 5.71e-16 77.8
MS.gene60640.t1 MTR_3g006370 32.738 168 107 5 18 181 12 177 7.45e-16 77.4
MS.gene60640.t1 MTR_8g446020 31.724 145 95 2 125 265 83 227 4.85e-15 75.9
MS.gene60640.t1 MTR_0402s0040 29.609 179 115 5 50 224 26 197 1.07e-14 77.0
MS.gene60640.t1 MTR_6g022070 30.496 141 97 1 263 402 29 169 8.81e-14 73.2
MS.gene60640.t1 MTR_2g014100 28.019 207 121 6 53 235 43 245 3.30e-13 70.1
MS.gene60640.t1 MTR_0853s0010 29.801 151 89 5 52 195 23 163 1.18e-12 66.6
MS.gene60640.t1 MTR_0337s0040 31.469 143 94 2 125 263 84 226 2.14e-12 67.4
MS.gene60640.t1 MTR_5g076070 23.005 213 157 4 57 265 56 265 6.56e-12 67.0
MS.gene60640.t1 MTR_1g019850 32.903 155 88 7 38 187 53 196 1.05e-11 65.5
MS.gene60640.t1 MTR_6g090505 24.913 289 164 9 101 357 22 289 1.05e-11 67.0
MS.gene60640.t1 MTR_7g446520 23.661 224 148 6 53 272 87 291 1.53e-11 66.2
MS.gene60640.t1 MTR_6g060130 49.206 63 31 1 15 76 60 122 7.22e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60640.t1 AT4G38180 41.060 302 173 5 256 555 397 695 2.95e-65 228
MS.gene60640.t1 AT4G38180 39.631 217 126 4 52 264 71 286 1.68e-37 148
MS.gene60640.t1 AT2G27110 34.364 291 182 4 268 556 210 493 6.12e-52 189
MS.gene60640.t1 AT2G27110 34.364 291 182 4 268 556 355 638 1.56e-51 190
MS.gene60640.t1 AT2G27110 28.638 213 126 5 53 265 48 234 1.10e-17 87.4
MS.gene60640.t1 AT2G27110 34.364 291 182 4 268 556 355 638 1.56e-51 190
MS.gene60640.t1 AT2G27110 28.638 213 126 5 53 265 48 234 1.10e-17 87.4
MS.gene60640.t1 AT4G38170 33.113 302 199 2 252 553 158 456 1.63e-51 185
MS.gene60640.t1 AT5G18960 42.358 229 129 1 266 494 503 728 8.63e-51 187
MS.gene60640.t1 AT5G18960 30.233 215 111 7 53 265 210 387 1.24e-20 96.7
MS.gene60640.t1 AT5G18960 29.078 141 67 4 52 174 41 166 1.59e-12 70.9
MS.gene60640.t1 AT5G18960 42.358 229 129 1 266 494 503 728 8.63e-51 187
MS.gene60640.t1 AT5G18960 30.233 215 111 7 53 265 210 387 1.24e-20 96.7
MS.gene60640.t1 AT5G18960 29.078 141 67 4 52 174 41 166 1.59e-12 70.9
MS.gene60640.t1 AT3G06250 40.611 229 133 1 266 494 479 704 8.92e-49 181
MS.gene60640.t1 AT3G06250 29.204 226 117 6 43 265 178 363 1.02e-22 103
MS.gene60640.t1 AT3G06250 32.456 114 61 2 44 156 18 116 3.03e-13 73.2
MS.gene60640.t1 AT3G06250 40.611 229 133 1 266 494 479 704 8.92e-49 181
MS.gene60640.t1 AT3G06250 29.204 226 117 6 43 265 178 363 1.02e-22 103
MS.gene60640.t1 AT3G06250 32.456 114 61 2 44 156 18 116 3.03e-13 73.2
MS.gene60640.t1 AT3G06250 40.611 229 133 1 266 494 479 704 8.92e-49 181
MS.gene60640.t1 AT3G06250 29.204 226 117 6 43 265 178 363 1.02e-22 103
MS.gene60640.t1 AT3G06250 32.456 114 61 2 44 156 18 116 3.03e-13 73.2
MS.gene60640.t1 AT3G22170 31.690 284 187 2 268 551 393 669 4.37e-47 177
MS.gene60640.t1 AT3G22170 26.160 237 140 7 52 275 68 282 4.67e-15 79.0
MS.gene60640.t1 AT3G22170 31.690 284 187 2 268 551 393 669 4.37e-47 177
MS.gene60640.t1 AT3G22170 26.160 237 140 7 52 275 68 282 4.67e-15 79.0
MS.gene60640.t1 AT4G15090 33.696 276 175 3 272 546 388 656 4.10e-46 174
MS.gene60640.t1 AT4G15090 31.651 218 142 5 52 265 49 263 3.38e-23 104
MS.gene60640.t1 AT4G15090 33.696 276 175 3 272 546 388 656 4.10e-46 174
MS.gene60640.t1 AT4G15090 31.651 218 142 5 52 265 49 263 3.38e-23 104
MS.gene60640.t1 AT4G15090 33.696 276 175 3 272 546 388 656 4.10e-46 174
MS.gene60640.t1 AT4G15090 31.651 218 142 5 52 265 49 263 3.38e-23 104
MS.gene60640.t1 AT4G15090 33.696 276 175 3 272 546 388 656 4.10e-46 174
MS.gene60640.t1 AT4G15090 31.651 218 142 5 52 265 49 263 3.38e-23 104
MS.gene60640.t1 AT4G15090 33.696 276 175 3 272 546 388 656 4.10e-46 174
MS.gene60640.t1 AT4G15090 31.651 218 142 5 52 265 49 263 3.38e-23 104
MS.gene60640.t1 AT1G52520 29.801 302 192 6 269 557 405 699 6.92e-39 152
MS.gene60640.t1 AT1G52520 26.636 214 142 4 54 265 85 285 1.99e-15 80.1
MS.gene60640.t1 AT1G76320 31.271 291 190 4 262 552 317 597 9.34e-38 149
MS.gene60640.t1 AT1G76320 26.293 232 149 8 58 283 1 216 5.64e-15 78.6
MS.gene60640.t1 AT1G76320 31.271 291 190 4 262 552 317 597 1.02e-37 149
MS.gene60640.t1 AT1G76320 26.293 232 149 8 58 283 1 216 5.75e-15 78.6
MS.gene60640.t1 AT1G76320 31.271 291 190 4 262 552 317 597 1.02e-37 149
MS.gene60640.t1 AT1G76320 26.293 232 149 8 58 283 1 216 5.75e-15 78.6
MS.gene60640.t1 AT1G76320 31.271 291 190 4 262 552 317 597 1.32e-37 148
MS.gene60640.t1 AT1G76320 25.847 236 145 7 58 283 1 216 4.79e-15 79.0
MS.gene60640.t1 AT4G19990 30.952 294 191 4 275 561 282 570 1.96e-34 139
MS.gene60640.t1 AT4G19990 30.952 294 191 4 275 561 353 641 2.44e-34 139
MS.gene60640.t1 AT4G19990 26.613 248 126 7 52 283 33 240 8.19e-18 87.8
MS.gene60640.t1 AT4G19990 30.952 294 191 4 275 561 339 627 2.69e-34 139
MS.gene60640.t1 AT4G19990 26.613 248 126 7 52 283 19 226 9.19e-18 87.4
MS.gene60640.t1 AT4G19990 30.952 294 191 4 275 561 339 627 2.70e-34 139
MS.gene60640.t1 AT4G19990 26.613 248 126 7 52 283 19 226 8.97e-18 87.8
MS.gene60640.t1 AT4G19990 30.952 294 191 4 275 561 339 627 2.70e-34 139
MS.gene60640.t1 AT4G19990 26.613 248 126 7 52 283 19 226 8.97e-18 87.8
MS.gene60640.t1 AT4G19990 30.952 294 191 4 275 561 339 627 3.11e-34 138
MS.gene60640.t1 AT4G19990 26.613 248 126 7 52 283 19 226 1.26e-17 87.0
MS.gene60640.t1 AT4G19990 30.952 294 191 4 275 561 339 627 3.11e-34 138
MS.gene60640.t1 AT4G19990 26.613 248 126 7 52 283 19 226 1.26e-17 87.0
MS.gene60640.t1 AT2G32250 31.557 244 164 2 256 497 316 558 1.83e-33 136
MS.gene60640.t1 AT2G32250 26.667 210 110 4 57 265 41 207 4.46e-12 69.7
MS.gene60640.t1 AT2G32250 31.557 244 164 2 256 497 316 558 1.83e-33 136
MS.gene60640.t1 AT2G32250 26.667 210 110 4 57 265 41 207 4.46e-12 69.7
MS.gene60640.t1 AT2G32250 31.557 244 164 2 256 497 316 558 1.83e-33 136
MS.gene60640.t1 AT2G32250 26.667 210 110 4 57 265 41 207 4.46e-12 69.7
MS.gene60640.t1 AT2G32250 31.557 244 164 2 256 497 275 517 1.87e-33 136
MS.gene60640.t1 AT2G32250 31.557 244 164 2 256 497 316 558 1.89e-33 136
MS.gene60640.t1 AT2G32250 27.143 210 109 4 57 265 41 207 4.16e-12 69.7
MS.gene60640.t1 AT2G32250 31.557 244 164 2 256 497 316 558 1.89e-33 136
MS.gene60640.t1 AT2G32250 27.143 210 109 4 57 265 41 207 4.16e-12 69.7
MS.gene60640.t1 AT2G32250 31.557 244 164 2 256 497 275 517 2.04e-33 136
MS.gene60640.t1 AT2G32250 31.557 244 164 2 256 497 316 558 2.21e-33 135
MS.gene60640.t1 AT2G32250 26.667 210 110 4 57 265 41 207 3.17e-12 70.1
MS.gene60640.t1 AT1G80010 27.243 301 206 5 264 556 425 720 7.50e-32 131
MS.gene60640.t1 AT1G80010 28.216 241 124 10 50 265 93 309 1.18e-14 77.8
MS.gene60640.t1 AT5G28530 25.468 267 193 2 282 548 415 675 1.74e-26 114
MS.gene60640.t1 AT5G28530 27.391 230 141 10 54 263 55 278 3.23e-11 66.6
MS.gene60640.t1 AT5G28530 25.843 267 192 3 282 548 352 612 1.84e-26 114
MS.gene60640.t1 AT1G10240 29.646 226 153 2 266 491 385 604 6.85e-26 112
MS.gene60640.t1 AT1G10240 23.656 279 181 11 17 271 1 271 6.54e-11 65.9
MS.gene60640.t1 AT1G10240 29.646 226 153 2 266 491 385 604 6.85e-26 112
MS.gene60640.t1 AT1G10240 23.656 279 181 11 17 271 1 271 6.54e-11 65.9
MS.gene60640.t1 AT5G28530 25.094 267 190 3 282 548 352 608 1.68e-25 111
MS.gene60640.t1 AT5G28530 24.719 267 191 2 282 548 415 671 1.74e-25 111
MS.gene60640.t1 AT5G28530 27.391 230 141 10 54 263 55 278 3.11e-11 66.6
MS.gene60640.t1 AT5G28530 24.719 267 191 2 282 548 415 671 1.74e-25 111
MS.gene60640.t1 AT5G28530 27.391 230 141 10 54 263 55 278 3.11e-11 66.6
MS.gene60640.t1 AT3G59470 38.053 113 58 3 53 160 69 174 1.42e-18 85.9
MS.gene60640.t1 AT3G59470 38.053 113 58 3 53 160 69 174 1.42e-18 85.9
MS.gene60640.t1 AT3G59470 38.053 113 58 3 53 160 81 186 1.79e-18 85.9
MS.gene60640.t1 AT3G07500 36.036 111 68 2 48 157 27 135 6.04e-15 74.7
MS.gene60640.t1 AT2G43280 34.959 123 77 3 43 163 11 132 1.49e-13 70.5
MS.gene60640.t1 AT4G12850 34.286 105 63 2 53 156 10 109 8.29e-13 66.2
MS.gene60640.t1 AT4G12850 34.286 105 63 2 53 156 10 109 1.06e-12 67.4
MS.gene60640.t1 AT4G12850 34.286 105 63 2 53 156 10 109 1.51e-12 65.9

Find 77 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGGCGGTATTGATAATTT+TGG 0.195979 1.2:+69847251 MS.gene60640:CDS
GAAAATTAACTAATTGCTTT+TGG 0.203998 1.2:+69847423 MS.gene60640:CDS
CATTCCTTCAATGGGCTCTA+AGG 0.236305 1.2:-69846811 None:intergenic
CAATACTGTTTGCGTTATTA+TGG 0.247980 1.2:+69846674 MS.gene60640:CDS
TGAAAAGTCACATGATATTT+AGG 0.266210 1.2:-69848325 None:intergenic
AATTGTCAGTTGAAGAATAC+TGG 0.271394 1.2:+69847368 MS.gene60640:CDS
CGAAGAGACTGTGCACTTCT+AGG 0.276999 1.2:-69847134 None:intergenic
AACTGTGTTCCTAAAGAAAA+AGG 0.312218 1.2:+69848420 MS.gene60640:CDS
AATCGTTTGGTATTCTTTGT+AGG 0.315386 1.2:+69848389 MS.gene60640:CDS
TATACTCAATTCGATCAAAA+TGG 0.317025 1.2:+69847933 MS.gene60640:CDS
TTCATTGTTTAATAGTGTTA+TGG 0.327096 1.2:+69848558 MS.gene60640:CDS
ATTCTTCAATTGTGATAGAT+TGG 0.351090 1.2:-69847844 None:intergenic
ACTGATGTGCTTGATGAATC+TGG 0.354525 1.2:+69847194 MS.gene60640:CDS
GAGAAAACAGCTGGTGATTA+TGG 0.357013 1.2:-69847112 None:intergenic
TGTCAAAAGTTTGAATCGTT+TGG 0.358658 1.2:+69848376 MS.gene60640:CDS
TTTAAACTCCATTCCTTCAA+TGG 0.364249 1.2:-69846820 None:intergenic
AGAACAAACGGTATGATAAT+TGG 0.370543 1.2:+69846927 MS.gene60640:CDS
TAGTGTTATGGTTCGTTCAC+TGG 0.382958 1.2:+69848570 MS.gene60640:CDS
ATTCCTTCAATGGGCTCTAA+GGG 0.384475 1.2:-69846810 None:intergenic
TTGAAGAATTTGAGTCTGAA+TGG 0.400537 1.2:+69847858 MS.gene60640:CDS
AGTAATTGATAACATCTTGT+TGG 0.416145 1.2:-69847280 None:intergenic
TTAAACTCCATTCCTTCAAT+GGG 0.435121 1.2:-69846819 None:intergenic
GTGCTTGATGAATCTGGTTT+AGG 0.447135 1.2:+69847200 MS.gene60640:CDS
TTGACAATTGAAGAATTTGA+AGG 0.456352 1.2:+69848514 MS.gene60640:CDS
TGAAGAATTTGAGTCTGAAT+GGG 0.463681 1.2:+69847859 MS.gene60640:CDS
TCCTTCAATGGGCTCTAAGG+GGG 0.466966 1.2:-69846808 None:intergenic
CTGGAATGTCTACCACTCAT+AGG 0.472850 1.2:+69847987 MS.gene60640:CDS
TTTGGCTCGAAACCCCTCCT+TGG 0.475441 1.2:-69846967 None:intergenic
TTTGAAGCTTCTTCTTCCAT+GGG 0.477189 1.2:-69848172 None:intergenic
ACTGTTTGCGTTATTATGGC+AGG 0.480651 1.2:+69846678 MS.gene60640:CDS
GAAGCAATGATTGATAAATA+CGG 0.502447 1.2:+69847881 MS.gene60640:CDS
AAATTATGATGTACATATGC+AGG 0.504291 1.2:-69847959 None:intergenic
GGGTATAAAGTTCGCAAAAG+TGG 0.509142 1.2:-69848151 None:intergenic
TCAAGAAATTCTTTGTTCCA+AGG 0.511520 1.2:+69846950 MS.gene60640:CDS
CCTTCAATGGGCTCTAAGGG+GGG 0.511902 1.2:-69846807 None:intergenic
AAGCAATGATTGATAAATAC+GGG 0.513517 1.2:+69847882 MS.gene60640:CDS
CACAGTATGTTCTACAACGA+TGG 0.518628 1.2:+69848458 MS.gene60640:CDS
TGTGTCAGAAACTTGCTCAA+TGG 0.523310 1.2:-69848058 None:intergenic
CTTAGAGCCCATTGAAGGAA+TGG 0.526994 1.2:+69846812 MS.gene60640:CDS
ACAATTGAAGAATTTGAAGG+AGG 0.530417 1.2:+69848517 MS.gene60640:CDS
CACTTCTAGGAGAAAACAGC+TGG 0.530595 1.2:-69847121 None:intergenic
TGAGATATTTCAAGATGAGT+TGG 0.531340 1.2:+69848216 MS.gene60640:CDS
GTTACTAAGTCAAGAACAAA+CGG 0.531428 1.2:+69846915 MS.gene60640:CDS
AAATTCTTTGTTCCAAGGAG+GGG 0.533751 1.2:+69846955 MS.gene60640:CDS
AGGTCATGAGATGACAGATA+TGG 0.541561 1.2:+69846698 MS.gene60640:CDS
GATGGTGGTTCACTATTGAG+AGG 0.542446 1.2:-69846780 None:intergenic
ATATTTCAAGATGAGTTGGT+TGG 0.542621 1.2:+69848220 MS.gene60640:CDS
ATTGTCAGTTGAAGAATACT+GGG 0.543680 1.2:+69847369 MS.gene60640:CDS
GAAATTCTTTGTTCCAAGGA+GGG 0.547884 1.2:+69846954 MS.gene60640:CDS
TGCACGTTACAACGAGGAAA+GGG 0.548977 1.2:+69848099 MS.gene60640:CDS
TGAAACCAGGGTGGGATGCA+AGG 0.555494 1.2:+69847031 MS.gene60640:CDS
TCTGTCTTATGCACTGAAAG+TGG 0.562246 1.2:+69847233 MS.gene60640:CDS
TTCCTTCAATGGGCTCTAAG+GGG 0.577393 1.2:-69846809 None:intergenic
CCGTGCCTTGCATCCCACCC+TGG 0.580963 1.2:-69847036 None:intergenic
TTTGCGAACTTTATACCCCA+TGG 0.583317 1.2:+69848156 MS.gene60640:CDS
ATGCACGTTACAACGAGGAA+AGG 0.585794 1.2:+69848098 MS.gene60640:CDS
CGTTGTAGAACATACTGTGA+AGG 0.588365 1.2:-69848454 None:intergenic
CATGAGATGACAGATATGGA+TGG 0.601625 1.2:+69846702 MS.gene60640:CDS
AGAAATTCTTTGTTCCAAGG+AGG 0.606491 1.2:+69846953 MS.gene60640:CDS
TTGAAGCTTCTTCTTCCATG+GGG 0.606500 1.2:-69848171 None:intergenic
GTTGTAGAACATACTGTGAA+GGG 0.621697 1.2:-69848453 None:intergenic
GCGTTTCAAATGGATGCCGA+AGG 0.622605 1.2:+69847401 MS.gene60640:CDS
ACAAGGTATGCTCAGAACAA+AGG 0.623611 1.2:+69846870 MS.gene60640:CDS
AGAGCTTGAAGAATCAAATG+AGG 0.626048 1.2:+69848681 MS.gene60640:CDS
TTATTACACATGATGAAACC+AGG 0.637175 1.2:+69847018 MS.gene60640:CDS
CATACTTTCACTCCTATGAG+TGG 0.639566 1.2:-69847999 None:intergenic
CCAGGGTGGGATGCAAGGCA+CGG 0.641442 1.2:+69847036 MS.gene60640:CDS
ACGGGTTGCAAGATAATCAG+TGG 0.648204 1.2:+69847900 MS.gene60640:CDS
AAGCAAAGAGTTACTACACA+AGG 0.651539 1.2:+69846853 MS.gene60640:CDS
ACACATGATGAAACCAGGGT+GGG 0.653979 1.2:+69847023 MS.gene60640:CDS
ACTTAGTAACACGGCCCATG+CGG 0.656322 1.2:-69846902 None:intergenic
GTCTTATGCACTGAAAGTGG+CGG 0.656902 1.2:+69847236 MS.gene60640:CDS
TTGTTCTTGACTTAGTAACA+CGG 0.665948 1.2:-69846911 None:intergenic
TACACATGATGAAACCAGGG+TGG 0.688016 1.2:+69847022 MS.gene60640:CDS
TATTACACATGATGAAACCA+GGG 0.694710 1.2:+69847019 MS.gene60640:CDS
TGTATCATTTGAGACAACCA+TGG 0.701765 1.2:-69846733 None:intergenic
TCTTGATGCACGTTACAACG+AGG 0.755842 1.2:+69848093 MS.gene60640:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAGAAAAATAATGATATA+TGG + chr1.2:69847066-69847085 MS.gene60640:CDS 10.0%
!!! AAAATTAACTAATTGCTTTT+GGG + chr1.2:69847424-69847443 MS.gene60640:CDS 15.0%
!!! AAGTATGAATAAGTTTTTTA+AGG + chr1.2:69848015-69848034 MS.gene60640:CDS 15.0%
!!! TATTTATTTTTCAAGTATGT+TGG - chr1.2:69847506-69847525 None:intergenic 15.0%
!! TTCATTGTTTAATAGTGTTA+TGG + chr1.2:69848558-69848577 MS.gene60640:CDS 20.0%
!!! ATTTTTCTTTCTTAGCATTT+CGG - chr1.2:69848486-69848505 None:intergenic 20.0%
!!! GAAAATTAACTAATTGCTTT+TGG + chr1.2:69847423-69847442 MS.gene60640:CDS 20.0%
! AAATTATGATGTACATATGC+AGG - chr1.2:69847962-69847981 None:intergenic 25.0%
! AAGCAATGATTGATAAATAC+GGG + chr1.2:69847882-69847901 MS.gene60640:CDS 25.0%
! ATTCTTCAATTGTGATAGAT+TGG - chr1.2:69847847-69847866 None:intergenic 25.0%
! GAAGCAATGATTGATAAATA+CGG + chr1.2:69847881-69847900 MS.gene60640:CDS 25.0%
! GAAGCTTCAAAAATTTATAC+AGG + chr1.2:69848184-69848203 MS.gene60640:CDS 25.0%
! GCTAAGAAAGAAAAATTTGA+AGG + chr1.2:69848490-69848509 MS.gene60640:CDS 25.0%
! TATACTCAATTCGATCAAAA+TGG + chr1.2:69847933-69847952 MS.gene60640:CDS 25.0%
! TGAAAAGTCACATGATATTT+AGG - chr1.2:69848328-69848347 None:intergenic 25.0%
! TTGACAATTGAAGAATTTGA+AGG + chr1.2:69848514-69848533 MS.gene60640:CDS 25.0%
!! AGAGTTGCTTACAAATATTT+TGG + chr1.2:69847461-69847480 MS.gene60640:intron 25.0%
!! AGTAATTGATAACATCTTGT+TGG - chr1.2:69847283-69847302 None:intergenic 25.0%
!! CATCAACAATCAATTCTTTT+TGG + chr1.2:69847563-69847582 MS.gene60640:intron 25.0%
!! GCAATTAGTTAATTTTCCTT+CGG - chr1.2:69847420-69847439 None:intergenic 25.0%
!! TTTTTCTTTCTTAGCATTTC+GGG - chr1.2:69848485-69848504 None:intergenic 25.0%
!!! AGAAAATTCAACTTTTTCTG+TGG - chr1.2:69848260-69848279 None:intergenic 25.0%
!!! TTACAAATATTTTGGTGATG+TGG + chr1.2:69847469-69847488 MS.gene60640:intron 25.0%
AACTGTGTTCCTAAAGAAAA+AGG + chr1.2:69848420-69848439 MS.gene60640:CDS 30.0%
AATCGTTTGGTATTCTTTGT+AGG + chr1.2:69848389-69848408 MS.gene60640:CDS 30.0%
AATTGTCAGTTGAAGAATAC+TGG + chr1.2:69847368-69847387 MS.gene60640:CDS 30.0%
ACAATTGAAGAATTTGAAGG+AGG + chr1.2:69848517-69848536 MS.gene60640:CDS 30.0%
AGAACAAACGGTATGATAAT+TGG + chr1.2:69846927-69846946 MS.gene60640:CDS 30.0%
ATTGTCAGTTGAAGAATACT+GGG + chr1.2:69847369-69847388 MS.gene60640:CDS 30.0%
CAATAATGGTGATTAACCTT+TGG - chr1.2:69847731-69847750 None:intergenic 30.0%
CAATACTGTTTGCGTTATTA+TGG + chr1.2:69846674-69846693 MS.gene60640:CDS 30.0%
GTTACTAAGTCAAGAACAAA+CGG + chr1.2:69846915-69846934 MS.gene60640:CDS 30.0%
TATTACACATGATGAAACCA+GGG + chr1.2:69847019-69847038 MS.gene60640:CDS 30.0%
TCAAGAAATTCTTTGTTCCA+AGG + chr1.2:69846950-69846969 MS.gene60640:CDS 30.0%
TGAAGAATTTGAGTCTGAAT+GGG + chr1.2:69847859-69847878 MS.gene60640:CDS 30.0%
TGAGATATTTCAAGATGAGT+TGG + chr1.2:69848216-69848235 MS.gene60640:CDS 30.0%
TGATGATTAACTCCAGTAAT+TGG - chr1.2:69847545-69847564 None:intergenic 30.0%
TGTCAAAAGTTTGAATCGTT+TGG + chr1.2:69848376-69848395 MS.gene60640:CDS 30.0%
TTAAACTCCATTCCTTCAAT+GGG - chr1.2:69846822-69846841 None:intergenic 30.0%
TTAATCACCATTATTGCATG+TGG + chr1.2:69847735-69847754 MS.gene60640:intron 30.0%
TTATTACACATGATGAAACC+AGG + chr1.2:69847018-69847037 MS.gene60640:CDS 30.0%
TTGAAGAATTTGAGTCTGAA+TGG + chr1.2:69847858-69847877 MS.gene60640:CDS 30.0%
TTGTTCTTGACTTAGTAACA+CGG - chr1.2:69846914-69846933 None:intergenic 30.0%
TTTAAACTCCATTCCTTCAA+TGG - chr1.2:69846823-69846842 None:intergenic 30.0%
! AGTTGAATTTTCTGATGAAG+TGG + chr1.2:69848267-69848286 MS.gene60640:CDS 30.0%
! GATAACGTACCTTTTTCTTT+AGG - chr1.2:69848432-69848451 None:intergenic 30.0%
! GTACATCATAATTTTTGTGC+TGG + chr1.2:69847968-69847987 MS.gene60640:CDS 30.0%
! GTTTTTCTATGCGTTTCAAA+TGG + chr1.2:69847391-69847410 MS.gene60640:CDS 30.0%
! TGGTCTGTGATAATAGTTTT+AGG - chr1.2:69847671-69847690 None:intergenic 30.0%
!! AATTTTCTGAAAGAGCTTCA+TGG + chr1.2:69848593-69848612 MS.gene60640:CDS 30.0%
!! ATATTTCAAGATGAGTTGGT+TGG + chr1.2:69848220-69848239 MS.gene60640:CDS 30.0%
!! GGTCTGTGATAATAGTTTTA+GGG - chr1.2:69847670-69847689 None:intergenic 30.0%
!! TCCTTGTTTCACATACTTTT+TGG - chr1.2:69846988-69847007 None:intergenic 30.0%
!!! AAACTGTAGAGAGTTTTGTT+TGG + chr1.2:69847606-69847625 MS.gene60640:intron 30.0%
AAATTCTTTGTTCCAAGGAG+GGG + chr1.2:69846955-69846974 MS.gene60640:CDS 35.0%
AAGCAAAGAGTTACTACACA+AGG + chr1.2:69846853-69846872 MS.gene60640:CDS 35.0%
ACTCCAGTAATTGGAACAAA+AGG - chr1.2:69847536-69847555 None:intergenic 35.0%
ACTTGGTTAGAAGCAATGAA+TGG + chr1.2:69847638-69847657 MS.gene60640:intron 35.0%
AGAAATTCTTTGTTCCAAGG+AGG + chr1.2:69846953-69846972 MS.gene60640:CDS 35.0%
AGAGCTTGAAGAATCAAATG+AGG + chr1.2:69848681-69848700 MS.gene60640:CDS 35.0%
CAATATGCCACATGCAATAA+TGG - chr1.2:69847745-69847764 None:intergenic 35.0%
CACATGAATCAGATCTATCA+TGG + chr1.2:69847782-69847801 MS.gene60640:intron 35.0%
CTTGGTTAGAAGCAATGAAT+GGG + chr1.2:69847639-69847658 MS.gene60640:intron 35.0%
GAAATTCTTTGTTCCAAGGA+GGG + chr1.2:69846954-69846973 MS.gene60640:CDS 35.0%
GTTGTAGAACATACTGTGAA+GGG - chr1.2:69848456-69848475 None:intergenic 35.0%
TTATTGCATGTGGCATATTG+AGG + chr1.2:69847745-69847764 MS.gene60640:intron 35.0%
TTGGTTAGAAGCAATGAATG+GGG + chr1.2:69847640-69847659 MS.gene60640:intron 35.0%
TTGTTTGGTTGCTAAGTACT+TGG + chr1.2:69847621-69847640 MS.gene60640:intron 35.0%
! ACTCCTTTTGTTCCAATTAC+TGG + chr1.2:69847530-69847549 MS.gene60640:intron 35.0%
! GCCAAAAAGTATGTGAAACA+AGG + chr1.2:69846984-69847003 MS.gene60640:CDS 35.0%
! TATGGATGGAGATTTTACCA+TGG + chr1.2:69846716-69846735 MS.gene60640:CDS 35.0%
! TGTATCATTTGAGACAACCA+TGG - chr1.2:69846736-69846755 None:intergenic 35.0%
! TTTGAAGCTTCTTCTTCCAT+GGG - chr1.2:69848175-69848194 None:intergenic 35.0%
!!! AAAGGTTTTAGTTTCCGCAT+GGG + chr1.2:69846888-69846907 MS.gene60640:CDS 35.0%
!!! AGTGGCGGTATTGATAATTT+TGG + chr1.2:69847251-69847270 MS.gene60640:CDS 35.0%
!!! ATTGCTTTTGGGTTGATTCA+AGG + chr1.2:69847435-69847454 MS.gene60640:CDS 35.0%
!!! TTTTGAAGCTTCTTCTTCCA+TGG - chr1.2:69848176-69848195 None:intergenic 35.0%
ACAAGGTATGCTCAGAACAA+AGG + chr1.2:69846870-69846889 MS.gene60640:CDS 40.0%
ACTGATGTGCTTGATGAATC+TGG + chr1.2:69847194-69847213 MS.gene60640:CDS 40.0%
ACTGTTTGCGTTATTATGGC+AGG + chr1.2:69846678-69846697 MS.gene60640:CDS 40.0%
AGGTCATGAGATGACAGATA+TGG + chr1.2:69846698-69846717 MS.gene60640:CDS 40.0%
CACAGTATGTTCTACAACGA+TGG + chr1.2:69848458-69848477 MS.gene60640:CDS 40.0%
CATACTTTCACTCCTATGAG+TGG - chr1.2:69848002-69848021 None:intergenic 40.0%
CATGAGATGACAGATATGGA+TGG + chr1.2:69846702-69846721 MS.gene60640:CDS 40.0%
CGTTGTAGAACATACTGTGA+AGG - chr1.2:69848457-69848476 None:intergenic 40.0%
GAGAAAACAGCTGGTGATTA+TGG - chr1.2:69847115-69847134 None:intergenic 40.0%
GGGTATAAAGTTCGCAAAAG+TGG - chr1.2:69848154-69848173 None:intergenic 40.0%
TAGATCTGATTCATGTGCTC+TGG - chr1.2:69847779-69847798 None:intergenic 40.0%
TAGTGTTATGGTTCGTTCAC+TGG + chr1.2:69848570-69848589 MS.gene60640:CDS 40.0%
TATCACCAATGCAGTTGCAA+GGG + chr1.2:69847700-69847719 MS.gene60640:intron 40.0%
TCTGTCTTATGCACTGAAAG+TGG + chr1.2:69847233-69847252 MS.gene60640:CDS 40.0%
TTGAAGCTTCTTCTTCCATG+GGG - chr1.2:69848174-69848193 None:intergenic 40.0%
TTTGCGAACTTTATACCCCA+TGG + chr1.2:69848156-69848175 MS.gene60640:CDS 40.0%
! ATTCCTTCAATGGGCTCTAA+GGG - chr1.2:69846813-69846832 None:intergenic 40.0%
! GTGCTTGATGAATCTGGTTT+AGG + chr1.2:69847200-69847219 MS.gene60640:CDS 40.0%
! TGTGTCAGAAACTTGCTCAA+TGG - chr1.2:69848061-69848080 None:intergenic 40.0%
!! TTTTGGTTGTGCTCTCTTGT+GGG + chr1.2:69847580-69847599 MS.gene60640:intron 40.0%
!!! CAAAGGTTTTAGTTTCCGCA+TGG + chr1.2:69846887-69846906 MS.gene60640:CDS 40.0%
!!! TTTTTGGTTGTGCTCTCTTG+TGG + chr1.2:69847579-69847598 MS.gene60640:intron 40.0%
AAACACCCTTGCAACTGCAT+TGG - chr1.2:69847708-69847727 None:intergenic 45.0%
ACACATGATGAAACCAGGGT+GGG + chr1.2:69847023-69847042 MS.gene60640:CDS 45.0%
ATGCACGTTACAACGAGGAA+AGG + chr1.2:69848098-69848117 MS.gene60640:CDS 45.0%
CTATCACCAATGCAGTTGCA+AGG + chr1.2:69847699-69847718 MS.gene60640:intron 45.0%
CTGGAATGTCTACCACTCAT+AGG + chr1.2:69847987-69848006 MS.gene60640:CDS 45.0%
CTTAGAGCCCATTGAAGGAA+TGG + chr1.2:69846812-69846831 MS.gene60640:CDS 45.0%
GTCTTATGCACTGAAAGTGG+CGG + chr1.2:69847236-69847255 MS.gene60640:CDS 45.0%
TACACATGATGAAACCAGGG+TGG + chr1.2:69847022-69847041 MS.gene60640:CDS 45.0%
TCTTGATGCACGTTACAACG+AGG + chr1.2:69848093-69848112 MS.gene60640:CDS 45.0%
TGCACGTTACAACGAGGAAA+GGG + chr1.2:69848099-69848118 MS.gene60640:CDS 45.0%
! ACGGGTTGCAAGATAATCAG+TGG + chr1.2:69847900-69847919 MS.gene60640:CDS 45.0%
! CACTTCTAGGAGAAAACAGC+TGG - chr1.2:69847124-69847143 None:intergenic 45.0%
! CATTCCTTCAATGGGCTCTA+AGG - chr1.2:69846814-69846833 None:intergenic 45.0%
! CATTGGTGATAGCAGTGTGT+TGG - chr1.2:69847691-69847710 None:intergenic 45.0%
! GATGGTGGTTCACTATTGAG+AGG - chr1.2:69846783-69846802 None:intergenic 45.0%
!! AGTTGCAAGGGTGTTTCCAA+AGG + chr1.2:69847712-69847731 MS.gene60640:intron 45.0%
!! TTCCTTCAATGGGCTCTAAG+GGG - chr1.2:69846812-69846831 None:intergenic 45.0%
ACTTAGTAACACGGCCCATG+CGG - chr1.2:69846905-69846924 None:intergenic 50.0%
CGAAGAGACTGTGCACTTCT+AGG - chr1.2:69847137-69847156 None:intergenic 50.0%
GCGTTTCAAATGGATGCCGA+AGG + chr1.2:69847401-69847420 MS.gene60640:CDS 50.0%
!! TCCTTCAATGGGCTCTAAGG+GGG - chr1.2:69846811-69846830 None:intergenic 50.0%
TGAAACCAGGGTGGGATGCA+AGG + chr1.2:69847031-69847050 MS.gene60640:CDS 55.0%
! TTTGGCTCGAAACCCCTCCT+TGG - chr1.2:69846970-69846989 None:intergenic 55.0%
!! CCTTCAATGGGCTCTAAGGG+GGG - chr1.2:69846810-69846829 None:intergenic 55.0%
CCCCCCTTAGAGCCCATTGA+AGG + chr1.2:69846807-69846826 MS.gene60640:CDS 60.0%
! TCTAAGGGGGGCTCTGATGG+TGG - chr1.2:69846798-69846817 None:intergenic 60.0%
CCAGGGTGGGATGCAAGGCA+CGG + chr1.2:69847036-69847055 MS.gene60640:CDS 65.0%
!! GGCTCTAAGGGGGGCTCTGA+TGG - chr1.2:69846801-69846820 None:intergenic 65.0%
CCGTGCCTTGCATCCCACCC+TGG - chr1.2:69847039-69847058 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 69846660 69848726 69846660 ID=MS.gene60640
chr1.2 mRNA 69846660 69848726 69846660 ID=MS.gene60640.t1;Parent=MS.gene60640
chr1.2 exon 69846660 69847456 69846660 ID=MS.gene60640.t1.exon1;Parent=MS.gene60640.t1
chr1.2 CDS 69846660 69847456 69846660 ID=cds.MS.gene60640.t1;Parent=MS.gene60640.t1
chr1.2 exon 69847811 69848726 69847811 ID=MS.gene60640.t1.exon2;Parent=MS.gene60640.t1
chr1.2 CDS 69847811 69848726 69847811 ID=cds.MS.gene60640.t1;Parent=MS.gene60640.t1
Gene Sequence

>MS.gene60640

ATGTTTAAAAGTCGCAATACTGTTTGCGTTATTATGGCAGGTCATGAGATGACAGATATGGATGGAGATTTTACCATGGTTGTCTCAAATGATACACATGTCAGTGCTCTTGATGTGAAGCCTCTCAATAGTGAACCACCATCAGAGCCCCCCTTAGAGCCCATTGAAGGAATGGAGTTTAAATCATTTGAAGAAGCAAAGAGTTACTACACAAGGTATGCTCAGAACAAAGGTTTTAGTTTCCGCATGGGCCGTGTTACTAAGTCAAGAACAAACGGTATGATAATTGGTCAAGAAATTCTTTGTTCCAAGGAGGGGTTTCGAGCCAAAAAGTATGTGAAACAAGGAAATAGTAGTCTTATTACACATGATGAAACCAGGGTGGGATGCAAGGCACGGTTATATTTAAAGAAAAATAATGATATATGGATTGTTTCTAGATTTGTAAGTGACCATAATCACCAGCTGTTTTCTCCTAGAAGTGCACAGTCTCTTCGCGTACATAGAAAGAAAACTAAAGTGCAAAAGACACTTACTGATGTGCTTGATGAATCTGGTTTAGGCAAAACAACATCTGTCTTATGCACTGAAAGTGGCGGTATTGATAATTTTGGATCTAGCCAACAAGATGTTATCAATTACTTAAGTGTACAGAGACAAAAACAATTTGAAAACAGAGATGCTCAATTGATGTTATCATACTTTAAGAATTGTCAGTTGAAGAATACTGGGTTTTTCTATGCGTTTCAAATGGATGCCGAAGGAAAATTAACTAATTGCTTTTGGGTTGATTCAAGGTCTAGAGTTGCTTACAAATATTTTGGTGATGTGGTTACTTTTGATCCAACATACTTGAAAAATAAATACAAAACTCCTTTTGTTCCAATTACTGGAGTTAATCATCATCAACAATCAATTCTTTTTGGTTGTGCTCTCTTGTGGGATGAAACTGTAGAGAGTTTTGTTTGGTTGCTAAGTACTTGGTTAGAAGCAATGAATGGGGTTTGCCCTAAAACTATTATCACAGACCAACACACTGCTATCACCAATGCAGTTGCAAGGGTGTTTCCAAAGGTTAATCACCATTATTGCATGTGGCATATTGAGGAAAAAGTTCCAGAGCACATGAATCAGATCTATCATGGACACAGTGAATTTAAAAATCATTTTTACAAGTGCATTCACCAATCTATCACAATTGAAGAATTTGAGTCTGAATGGGAAGCAATGATTGATAAATACGGGTTGCAAGATAATCAGTGGCTAGTGAAGATATACTCAATTCGATCAAAATGGATTCCTGCATATGTACATCATAATTTTTGTGCTGGAATGTCTACCACTCATAGGAGTGAAAGTATGAATAAGTTTTTTAAGGATTTCTTGAATTCAAGTACTCCATTGAGCAAGTTTCTGACACAATATGAGAAAGCTCTTGATGCACGTTACAACGAGGAAAGGGAGAAAACTGTCAAGACGATGAATTCAAAACCACTTTTGCGAACTTTATACCCCATGGAAGAAGAAGCTTCAAAAATTTATACAGGAAAATTGTTTGAGATATTTCAAGATGAGTTGGTTGGCTCTCAAATGTTTACCACAGAAAAAGTTGAATTTTCTGATGAAGTGGCAACATACAAAGTTCATGAAATTTACAAAGAGAAGCCTAAATATCATGTGACTTTTCATGTAACTTCAAAAGAAGCAACTTGTACTTGTCAAAAGTTTGAATCGTTTGGTATTCTTTGTAGGCATATCTTAACTGTGTTCCTAAAGAAAAAGGTACGTTATCTCCCTTCACAGTATGTTCTACAACGATGGACCCGAAATGCTAAGAAAGAAAAATTTGAAGGATTGACAATTGAAGAATTTGAAGGAGGAAACAAAGCTTCTAGCACTTCATTGTTTAATAGTGTTATGGTTCGTTCACTGGAATTTTCTGAAAGAGCTTCATGGTCAAAAAAACATCATGATATTGCAATTCAATGTTTGCAAAATGCAATTTCAAAACTTGATCTATTAGAGCTTGAAGAATCAAATGAGGAGTTTGTTAATTTGACATCTTAA
Protein sequence

>MS.gene60640.t1

MFKSRNTVCVIMAGHEMTDMDGDFTMVVSNDTHVSALDVKPLNSEPPSEPPLEPIEGMEFKSFEEAKSYYTRYAQNKGFSFRMGRVTKSRTNGMIIGQEILCSKEGFRAKKYVKQGNSSLITHDETRVGCKARLYLKKNNDIWIVSRFVSDHNHQLFSPRSAQSLRVHRKKTKVQKTLTDVLDESGLGKTTSVLCTESGGIDNFGSSQQDVINYLSVQRQKQFENRDAQLMLSYFKNCQLKNTGFFYAFQMDAEGKLTNCFWVDSSEFKNHFYKCIHQSITIEEFESEWEAMIDKYGLQDNQWLVKIYSIRSKWIPAYVHHNFCAGMSTTHRSESMNKFFKDFLNSSTPLSKFLTQYEKALDARYNEEREKTVKTMNSKPLLRTLYPMEEEASKIYTGKLFEIFQDELVGSQMFTTEKVEFSDEVATYKVHEIYKEKPKYHVTFHVTSKEATCTCQKFESFGILCRHILTVFLKKKVRYLPSQYVLQRWTRNAKKEKFEGLTIEEFEGGNKASSTSLFNSVMVRSLEFSERASWSKKHHDIAIQCLQNAISKLDLLELEESNEEFVNLTS