AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60682

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60682.t1 MTR_1g098870 96.071 560 19 1 1 560 9 565 0.0 1117
MS.gene60682.t1 MTR_1g098900 47.469 573 186 13 18 492 29 584 4.00e-153 453
MS.gene60682.t1 MTR_6g035370 53.819 288 112 3 44 310 52 339 1.61e-73 246
MS.gene60682.t1 MTR_6g035370 62.879 132 43 2 355 483 449 577 5.92e-46 171
MS.gene60682.t1 MTR_6g035370 43.617 94 52 1 43 135 460 553 1.14e-18 90.1
MS.gene60682.t1 MTR_6g035370 41.379 87 50 1 367 453 55 140 7.51e-17 84.3
MS.gene60682.t1 MTR_1g492790 30.118 425 222 13 28 424 95 472 1.37e-46 171
MS.gene60682.t1 MTR_1g492790 38.129 139 81 3 321 458 71 205 4.75e-20 94.0
MS.gene60682.t1 MTR_2g026725 37.860 243 123 4 233 455 116 350 1.06e-43 160
MS.gene60682.t1 MTR_2g026725 39.091 110 59 2 46 154 261 363 5.36e-16 80.5
MS.gene60682.t1 MTR_1g060990 34.014 294 162 6 47 335 131 397 5.16e-41 155
MS.gene60682.t1 MTR_1g060990 42.202 109 61 2 356 463 120 227 3.24e-20 94.4
MS.gene60682.t1 MTR_1g081180 33.835 266 125 6 231 454 251 507 2.32e-33 134
MS.gene60682.t1 MTR_1g081180 40.426 94 53 2 45 135 416 509 4.90e-14 75.1
MS.gene60682.t1 MTR_7g114860 31.174 247 142 7 232 457 241 480 3.66e-29 121
MS.gene60682.t1 MTR_7g114860 43.434 99 46 2 47 135 381 479 4.90e-16 81.3
MS.gene60682.t1 MTR_5g026540 30.075 133 81 3 327 457 10 132 1.43e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60682.t1 AT1G76890 49.593 492 182 10 42 488 36 506 3.05e-141 421
MS.gene60682.t1 AT1G76880 52.391 481 193 8 42 489 56 533 1.60e-137 412
MS.gene60682.t1 AT5G03680 34.038 426 235 12 47 454 119 516 5.73e-60 209
MS.gene60682.t1 AT5G28300 37.838 259 127 6 232 456 300 558 4.07e-40 154
MS.gene60682.t1 AT3G10000 33.557 298 143 8 41 315 82 347 1.75e-38 147
MS.gene60682.t1 AT3G10000 35.915 142 81 3 335 469 53 191 6.12e-21 96.7
MS.gene60682.t1 AT1G33240 68.132 91 29 0 367 457 435 525 1.13e-37 147
MS.gene60682.t1 AT1G33240 68.132 91 29 0 367 457 396 486 1.21e-37 147
MS.gene60682.t1 AT1G33240 43.678 87 48 1 47 132 396 482 2.01e-16 83.2
MS.gene60682.t1 AT1G33240 68.132 91 29 0 367 457 435 525 2.26e-37 147
MS.gene60682.t1 AT3G10000 32.323 297 138 8 41 315 82 337 9.99e-35 137
MS.gene60682.t1 AT3G10000 34.043 141 75 3 335 469 53 181 7.99e-18 86.7
MS.gene60682.t1 AT5G47660 31.718 227 140 4 230 455 177 389 1.62e-27 115
MS.gene60682.t1 AT5G47660 41.304 92 51 2 47 137 303 392 4.45e-13 71.6
MS.gene60682.t1 AT3G25990 32.000 100 67 1 368 466 55 154 5.97e-11 64.7

Find 117 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTATAGAAAGAAGAGAAAA+TGG 0.189121 1.2:-70692120 MS.gene60682:CDS
GAACCAAAGTATCAAGAAAA+TGG 0.192524 1.2:-70691641 MS.gene60682:CDS
TTCATGTTTGGCAAAGAATT+TGG 0.196846 1.2:+70692211 None:intergenic
TTATTATTTGGTTGTGGTTT+TGG 0.205052 1.2:+70692364 None:intergenic
GAAAATGGAAGGACTATTTC+AGG 0.211748 1.2:-70692105 MS.gene60682:CDS
GGAGGTCTCCATTGTTGTTC+TGG 0.263104 1.2:+70691338 None:intergenic
TCTTCCCACAATGGCGCTTT+TGG 0.281382 1.2:+70691617 None:intergenic
GAATTTCAAAATATGAATAA+TGG 0.294434 1.2:-70691755 MS.gene60682:CDS
GAGCTGCTGATGATCTTTCT+TGG 0.298176 1.2:+70691930 None:intergenic
GTGAAGTTCATGCTCTAATA+AGG 0.301161 1.2:-70691679 MS.gene60682:CDS
GCTTCAAGTTCATTAAAATA+AGG 0.305695 1.2:+70691458 None:intergenic
GATGAGAGTGTGTTATTATT+TGG 0.316885 1.2:+70692352 None:intergenic
TCAAGAGAGCCAATGTTTCT+TGG 0.332675 1.2:+70693439 None:intergenic
GAGAATAGGTTGTTCATGTT+TGG 0.353362 1.2:+70692199 None:intergenic
TTAACGGTGGTGGAGGAAAT+AGG 0.356278 1.2:-70693470 MS.gene60682:CDS
AACTTCTTCTTCCACAGCTT+CGG 0.361209 1.2:-70692161 MS.gene60682:CDS
GAAACGAATTAGAACCAAAA+AGG 0.368019 1.2:+70691405 None:intergenic
TATTGTTTGTGTGGTTTCAT+TGG 0.368826 1.2:+70692315 None:intergenic
CTTGAAGATAAGATCAGATA+TGG 0.382626 1.2:-70693421 MS.gene60682:CDS
GCTGGCGGTGGTTGTGGTGG+TGG 0.395680 1.2:+70691860 None:intergenic
AAGTCTTAAGGGTCCACTTT+GGG 0.401378 1.2:-70693379 MS.gene60682:CDS
TCTGGTGCTGGCGGTGGTTG+TGG 0.405288 1.2:+70691854 None:intergenic
AACGAATGTGGTAGGGTTTG+TGG 0.409596 1.2:+70692267 None:intergenic
GAAGAGAAAGGAAGAGAGAT+TGG 0.412336 1.2:-70693525 MS.gene60682:CDS
CACAGCTTCGGACGAAGACT+TGG 0.417011 1.2:-70692149 MS.gene60682:CDS
TGTTCTGGCTGCACCATCAA+TGG 0.420164 1.2:+70691353 None:intergenic
ATTGTTTGTGTGGTTTCATT+GGG 0.421975 1.2:+70692316 None:intergenic
ATGAATAATGGTTACAAAAG+TGG 0.422836 1.2:-70691743 MS.gene60682:CDS
GTTTCATTGGGATTTATTGT+TGG 0.423348 1.2:+70692328 None:intergenic
TGCTTTGAGAATTCAAGAAA+TGG 0.425606 1.2:-70691984 MS.gene60682:CDS
AATTCAACAATGTTGTTGTT+TGG 0.432386 1.2:+70691773 None:intergenic
TTTGGAACATTGTTGTTGTT+AGG 0.434536 1.2:+70692229 None:intergenic
CACTTTGGGAAGAGGTTTCA+AGG 0.436137 1.2:-70693365 MS.gene60682:intron
AACAAATAAACTTTCATCAA+TGG 0.444317 1.2:-70691144 MS.gene60682:CDS
GACAGCTCTGGTGCTGGCGG+TGG 0.444859 1.2:+70691848 None:intergenic
AAGTTGTTGTGGTTGAGAAT+AGG 0.446298 1.2:+70692185 None:intergenic
AACCAAAGTATCAAGAAAAT+GGG 0.447699 1.2:-70691640 MS.gene60682:CDS
TGAAGAGACAAGGATACAAT+AGG 0.451641 1.2:-70691580 MS.gene60682:CDS
ATCAATATTAACGGTGGTGG+AGG 0.457605 1.2:-70693477 MS.gene60682:CDS
GTGCTTCTCCTTCTCCATCA+AGG 0.462199 1.2:-70691712 MS.gene60682:CDS
GAGAGCCAATGTTTCTTGGC+GGG 0.464598 1.2:+70693443 None:intergenic
AATGGTTACAAAAGTGGTAA+TGG 0.466799 1.2:-70691737 MS.gene60682:CDS
TGAGAATATTGTGGCGGATA+CGG 0.467895 1.2:-70693568 MS.gene60682:CDS
CACGGAGACAGCTCTGGTGC+TGG 0.472655 1.2:+70691842 None:intergenic
TAAGGGTCCACTTTGGGAAG+AGG 0.472903 1.2:-70693373 MS.gene60682:CDS
TTTCGTGATTCAAGTCTTAA+GGG 0.478392 1.2:-70693390 MS.gene60682:CDS
AAGATGAATATCAATATTAA+CGG 0.478883 1.2:-70693486 MS.gene60682:CDS
ACATTGTTGTTGTTAGGTTG+TGG 0.486612 1.2:+70692235 None:intergenic
AGGAAGAGAGATTGGTGGTG+AGG 0.490326 1.2:-70693517 MS.gene60682:CDS
ACGGCGCAAAACGGAGGCGG+CGG 0.492141 1.2:-70693549 MS.gene60682:CDS
TGAGACGATGGAGCCATTGA+TGG 0.495814 1.2:-70691366 MS.gene60682:CDS
CAAGTCTTAAGGGTCCACTT+TGG 0.495967 1.2:-70693380 MS.gene60682:CDS
GGTGCTGGCGGTGGTTGTGG+TGG 0.502284 1.2:+70691857 None:intergenic
GATACGGCGCAAAACGGAGG+CGG 0.503294 1.2:-70693552 MS.gene60682:CDS
GTCTGACACGGAGACAGCTC+TGG 0.505511 1.2:+70691836 None:intergenic
AATATCAATATTAACGGTGG+TGG 0.506001 1.2:-70693480 MS.gene60682:CDS
CCCTCTTCAAATGTAGTTGG+AGG 0.507603 1.2:+70691320 None:intergenic
TGTAGGAAACTAGCTGAACT+TGG 0.509833 1.2:-70692550 MS.gene60682:intron
GTCTCCGTGTCAGACAGCTC+TGG 0.513072 1.2:-70691828 MS.gene60682:CDS
TAGAAAGAAGAGAAAATGGA+AGG 0.520671 1.2:-70692116 MS.gene60682:CDS
GAAGGAGAAACGAATGTGGT+AGG 0.521206 1.2:+70692259 None:intergenic
GTACCCTCTTCAAATGTAGT+TGG 0.522241 1.2:+70691317 None:intergenic
TGGGCCAAAAGCGCCATTGT+GGG 0.524060 1.2:-70691621 MS.gene60682:CDS
AGGGTTTGTGGATGGAACAG+TGG 0.526949 1.2:+70692279 None:intergenic
CAAGTCTTCGTCCGAAGCTG+TGG 0.529430 1.2:+70692150 None:intergenic
GATAGCATGGAAGAAGATGA+AGG 0.531181 1.2:-70691197 MS.gene60682:CDS
AGTGTGTTATTATTTGGTTG+TGG 0.532548 1.2:+70692358 None:intergenic
GACATATGATATTGTTTGTG+TGG 0.533682 1.2:+70692306 None:intergenic
TTTGGCCACCTTGATGGAGA+AGG 0.538200 1.2:+70691704 None:intergenic
TATCACAAAAGAACCAAAGA+AGG 0.539271 1.2:-70692475 MS.gene60682:CDS
GTGGCGGATACGGCGCAAAA+CGG 0.541031 1.2:-70693558 MS.gene60682:CDS
ATATGAAGATGGAGATAGCA+TGG 0.544034 1.2:-70691210 MS.gene60682:CDS
AGAGCCAATGTTTCTTGGCG+GGG 0.547860 1.2:+70693444 None:intergenic
CTTGAAACCTCTTCCCAAAG+TGG 0.553632 1.2:+70693366 None:intergenic
GGTAGGAGTGGAAAATCAGA+AGG 0.553996 1.2:-70692454 MS.gene60682:CDS
ATGGGCCAAAAGCGCCATTG+TGG 0.554967 1.2:-70691622 MS.gene60682:CDS
GAGAAAGGAAGAGAGATTGG+TGG 0.555860 1.2:-70693522 MS.gene60682:CDS
TTGTTGTTAGGTTGTGGTGA+AGG 0.559792 1.2:+70692241 None:intergenic
GGAGGCGGCGGTGAAGAGAA+AGG 0.561124 1.2:-70693537 MS.gene60682:CDS
TTGTTGTCACAACTTCATAA+CGG 0.563283 1.2:+70691162 None:intergenic
AACATGGATGAATATGAAGA+TGG 0.566553 1.2:-70691221 MS.gene60682:CDS
AGAAGAAGTTGAAGTTGTTG+TGG 0.571331 1.2:+70692174 None:intergenic
AAGGAGAAACGAATGTGGTA+GGG 0.571339 1.2:+70692260 None:intergenic
AGAGAGCCAATGTTTCTTGG+CGG 0.579042 1.2:+70693442 None:intergenic
CAAATACTACAAGAAAATGA+AGG 0.579174 1.2:-70691519 MS.gene60682:CDS
TGATGATGAAGATGAAAACA+TGG 0.579902 1.2:-70691237 MS.gene60682:CDS
GTGGCCCCGCCAAGAAACAT+TGG 0.588347 1.2:-70693448 MS.gene60682:CDS
GGAGACAGCTCTGGTGCTGG+CGG 0.588687 1.2:+70691845 None:intergenic
GATACAATAGGAATGCGAAA+AGG 0.589387 1.2:-70691568 MS.gene60682:CDS
AGAACCAAAGAAGGTAGGAG+TGG 0.596126 1.2:-70692466 MS.gene60682:CDS
ACCTCCAACTACATTTGAAG+AGG 0.596492 1.2:-70691321 MS.gene60682:CDS
AAGATAAGATCAGATATGGA+TGG 0.598844 1.2:-70693417 MS.gene60682:CDS
TGAAGGAAAGTAATAAGCAA+AGG 0.602677 1.2:-70691502 MS.gene60682:CDS
ATCCGCCACAATATTCTCAC+CGG 0.602769 1.2:+70693572 None:intergenic
AATGCAAAAGAAGTTTCTTG+AGG 0.603327 1.2:-70692047 MS.gene60682:CDS
ACAAGTGCTTGCAAAACAAG+AGG 0.605374 1.2:-70692071 MS.gene60682:CDS
GTAATGATGGAGACTTGCTC+CGG 0.605776 1.2:-70693591 MS.gene60682:CDS
TAGAAGCTCAAAGAAGTGCA+AGG 0.606310 1.2:-70692521 MS.gene60682:CDS
ACAAAAGAACCAAAGAAGGT+AGG 0.608634 1.2:-70692471 MS.gene60682:CDS
TGGTGCAGCCAGAACAACAA+TGG 0.627830 1.2:-70691346 MS.gene60682:CDS
TTGCTCCGGTGAGAATATTG+TGG 0.640151 1.2:-70693577 MS.gene60682:CDS
GCAAAAGAAGAAGATGACAA+TGG 0.642786 1.2:-70691269 MS.gene60682:CDS
GCGGATACGGCGCAAAACGG+AGG 0.646418 1.2:-70693555 MS.gene60682:CDS
AGCATGGAAGAAGATGAAGG+AGG 0.648293 1.2:-70691194 MS.gene60682:CDS
ATGAATATCAATATTAACGG+TGG 0.649447 1.2:-70693483 MS.gene60682:CDS
CCTCCAACTACATTTGAAGA+GGG 0.651540 1.2:-70691320 MS.gene60682:CDS
CTCCGGTGAGAATATTGTGG+CGG 0.652038 1.2:-70693574 MS.gene60682:CDS
CTTGAAGTCAAATGAGACGA+TGG 0.663039 1.2:-70691378 MS.gene60682:CDS
TACATTTGAAGAGGGTACTG+TGG 0.663962 1.2:-70691312 MS.gene60682:CDS
GCTGAAATATCTTCCCACAA+TGG 0.664092 1.2:+70691608 None:intergenic
GGTTACAAAAGTGGTAATGG+TGG 0.667767 1.2:-70691734 MS.gene60682:CDS
AATGTGGTAGGGTTTGTGGA+TGG 0.673197 1.2:+70692271 None:intergenic
CTTCTCCTTCTCCATCAAGG+TGG 0.673995 1.2:-70691709 MS.gene60682:CDS
TCAGCTGCAATGAAGAGACA+AGG 0.690286 1.2:-70691590 MS.gene60682:CDS
GATGCCAGAGCTGTCTGACA+CGG 0.707672 1.2:+70691824 None:intergenic
TGGTGAAGGAGAAACGAATG+TGG 0.713404 1.2:+70692255 None:intergenic
ACGGTGGTGGAGGAAATAGG+TGG 0.747281 1.2:-70693467 MS.gene60682:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAACTTAAGTAATTTAAT+TGG + chr1.2:70691792-70691811 None:intergenic 10.0%
!! TTGGTTAATAAAATTATATA+TGG + chr1.2:70691773-70691792 None:intergenic 10.0%
!!! TGTGTTTATTTTTTTTTTTT+TGG - chr1.2:70691931-70691950 MS.gene60682:CDS 10.0%
!! AAGATGAATATCAATATTAA+CGG - chr1.2:70691232-70691251 MS.gene60682:CDS 15.0%
!! ACAAAGTAATAAAAATTAGA+TGG + chr1.2:70691861-70691880 None:intergenic 15.0%
!! GAATTTCAAAATATGAATAA+TGG - chr1.2:70692963-70692982 MS.gene60682:intron 15.0%
!! TAATTGATCAAAAAATCTAT+AGG + chr1.2:70692295-70692314 None:intergenic 15.0%
!! AAAAGTAAAAATTTGTGATG+AGG + chr1.2:70692000-70692019 None:intergenic 20.0%
!! AACAAATAAACTTTCATCAA+TGG - chr1.2:70693574-70693593 MS.gene60682:CDS 20.0%
!! AGTAATAAAAATTAGATGGA+AGG + chr1.2:70691857-70691876 None:intergenic 20.0%
!! CTTATAAACAAGAAAATGAA+AGG + chr1.2:70691457-70691476 None:intergenic 20.0%
!!! ATTTTTTTTTCCTTCTGAAA+AGG - chr1.2:70691593-70691612 MS.gene60682:CDS 20.0%
!!! TTTGTGTGTTTCTTATTTAT+TGG - chr1.2:70691876-70691895 MS.gene60682:CDS 20.0%
!!! TTTTACTTTTTTTACGTGAA+AGG - chr1.2:70692010-70692029 MS.gene60682:CDS 20.0%
!!! TTTTTCTTTTCCTTTTTCAA+TGG - chr1.2:70692041-70692060 MS.gene60682:CDS 20.0%
! AAAAATAAACACATGAAGCT+AGG + chr1.2:70691924-70691943 None:intergenic 25.0%
! AGTATAGAAAGAAGAGAAAA+TGG - chr1.2:70692598-70692617 MS.gene60682:intron 25.0%
! ATGAATAATGGTTACAAAAG+TGG - chr1.2:70692975-70692994 MS.gene60682:intron 25.0%
! ATGAATATCAATATTAACGG+TGG - chr1.2:70691235-70691254 MS.gene60682:CDS 25.0%
! CAAATACTACAAGAAAATGA+AGG - chr1.2:70693199-70693218 MS.gene60682:intron 25.0%
! CCTACATGTATATATATAGA+GGG + chr1.2:70692154-70692173 None:intergenic 25.0%
! GTTAGTATATCCATTGAAAA+AGG + chr1.2:70692054-70692073 None:intergenic 25.0%
! TCCTACATGTATATATATAG+AGG + chr1.2:70692155-70692174 None:intergenic 25.0%
!! AACCAAAGTATCAAGAAAAT+GGG - chr1.2:70693078-70693097 MS.gene60682:intron 25.0%
!! AATTCAACAATGTTGTTGTT+TGG + chr1.2:70692948-70692967 None:intergenic 25.0%
!! CAAAAACTGATTGGTTAATT+TGG + chr1.2:70691520-70691539 None:intergenic 25.0%
!! CAAATTAACCAATCAGTTTT+TGG - chr1.2:70691518-70691537 MS.gene60682:CDS 25.0%
!! GCTTCAAGTTCATTAAAATA+AGG + chr1.2:70693263-70693282 None:intergenic 25.0%
!! TATTTATTGGTCACTCTTTT+AGG - chr1.2:70691889-70691908 MS.gene60682:CDS 25.0%
!! TTATTATTTGGTTGTGGTTT+TGG + chr1.2:70692357-70692376 None:intergenic 25.0%
!!! CATCTTCTTCAACTTTTTTT+AGG - chr1.2:70691821-70691840 MS.gene60682:CDS 25.0%
AACATGGATGAATATGAAGA+TGG - chr1.2:70693497-70693516 MS.gene60682:CDS 30.0%
AAGATAAGATCAGATATGGA+TGG - chr1.2:70691301-70691320 MS.gene60682:CDS 30.0%
AATAGGTTCCAAAAACTGAT+TGG + chr1.2:70691529-70691548 None:intergenic 30.0%
AATATCAATATTAACGGTGG+TGG - chr1.2:70691238-70691257 MS.gene60682:CDS 30.0%
AATGCAAAAGAAGTTTCTTG+AGG - chr1.2:70692671-70692690 MS.gene60682:intron 30.0%
AATGGTTACAAAAGTGGTAA+TGG - chr1.2:70692981-70693000 MS.gene60682:intron 30.0%
ATCTTATTCTCAAGTGTATG+AGG - chr1.2:70691621-70691640 MS.gene60682:CDS 30.0%
ATTGTTTGTGTGGTTTCATT+GGG + chr1.2:70692405-70692424 None:intergenic 30.0%
CCCTCTATATATATACATGT+AGG - chr1.2:70692151-70692170 MS.gene60682:CDS 30.0%
CTTGAAGATAAGATCAGATA+TGG - chr1.2:70691297-70691316 MS.gene60682:CDS 30.0%
GAAACGAATTAGAACCAAAA+AGG + chr1.2:70693316-70693335 None:intergenic 30.0%
GACATATGATATTGTTTGTG+TGG + chr1.2:70692415-70692434 None:intergenic 30.0%
GTATATATATAGAGGGATCA+AGG + chr1.2:70692147-70692166 None:intergenic 30.0%
GTCAAAAAAAGCTCTTTGAT+TGG - chr1.2:70691566-70691585 MS.gene60682:CDS 30.0%
GTTTCATTGGGATTTATTGT+TGG + chr1.2:70692393-70692412 None:intergenic 30.0%
TAGAAAGAAGAGAAAATGGA+AGG - chr1.2:70692602-70692621 MS.gene60682:intron 30.0%
TATCACAAAAGAACCAAAGA+AGG - chr1.2:70692243-70692262 MS.gene60682:CDS 30.0%
TATTGTTTGTGTGGTTTCAT+TGG + chr1.2:70692406-70692425 None:intergenic 30.0%
TGAAGGAAAGTAATAAGCAA+AGG - chr1.2:70693216-70693235 MS.gene60682:intron 30.0%
TTCATGTTTGGCAAAGAATT+TGG + chr1.2:70692510-70692529 None:intergenic 30.0%
TTGTTGTCACAACTTCATAA+CGG + chr1.2:70693559-70693578 None:intergenic 30.0%
TTTCGTGATTCAAGTCTTAA+GGG - chr1.2:70691328-70691347 MS.gene60682:CDS 30.0%
! AACAAAACACAGAACCTTTT+TGG - chr1.2:70693299-70693318 MS.gene60682:intron 30.0%
! AAGATATATGCCTTTTCAGA+AGG + chr1.2:70691606-70691625 None:intergenic 30.0%
! AGTGTGTTATTATTTGGTTG+TGG + chr1.2:70692363-70692382 None:intergenic 30.0%
! GAAAAGGTGCAAAGAAAAAT+GGG - chr1.2:70693166-70693185 MS.gene60682:intron 30.0%
! GAACCAAAGTATCAAGAAAA+TGG - chr1.2:70693077-70693096 MS.gene60682:intron 30.0%
! GATGAGAGTGTGTTATTATT+TGG + chr1.2:70692369-70692388 None:intergenic 30.0%
! TGATGATGAAGATGAAAACA+TGG - chr1.2:70693481-70693500 MS.gene60682:CDS 30.0%
! TTTGGAACATTGTTGTTGTT+AGG + chr1.2:70692492-70692511 None:intergenic 30.0%
! TTTTCGTGATTCAAGTCTTA+AGG - chr1.2:70691327-70691346 MS.gene60682:CDS 30.0%
!! TGCTTTGAGAATTCAAGAAA+TGG - chr1.2:70692734-70692753 MS.gene60682:intron 30.0%
AAGTTGTTGTGGTTGAGAAT+AGG + chr1.2:70692536-70692555 None:intergenic 35.0%
ACAAAAGAACCAAAGAAGGT+AGG - chr1.2:70692247-70692266 MS.gene60682:CDS 35.0%
AGAAGAAGTTGAAGTTGTTG+TGG + chr1.2:70692547-70692566 None:intergenic 35.0%
ATATGAAGATGGAGATAGCA+TGG - chr1.2:70693508-70693527 MS.gene60682:CDS 35.0%
GAAAATGGAAGGACTATTTC+AGG - chr1.2:70692613-70692632 MS.gene60682:intron 35.0%
GAGAATAGGTTGTTCATGTT+TGG + chr1.2:70692522-70692541 None:intergenic 35.0%
GATACAATAGGAATGCGAAA+AGG - chr1.2:70693150-70693169 MS.gene60682:intron 35.0%
GCAAAAGAAGAAGATGACAA+TGG - chr1.2:70693449-70693468 MS.gene60682:CDS 35.0%
GCATTTCTCCAAAAATTGTC+TGG - chr1.2:70692831-70692850 MS.gene60682:intron 35.0%
GTGAAGTTCATGCTCTAATA+AGG - chr1.2:70693039-70693058 MS.gene60682:intron 35.0%
TAATTCCACTGACAGGAAAT+AGG + chr1.2:70691546-70691565 None:intergenic 35.0%
TGAAGAGACAAGGATACAAT+AGG - chr1.2:70693138-70693157 MS.gene60682:intron 35.0%
! ACATTGTTGTTGTTAGGTTG+TGG + chr1.2:70692486-70692505 None:intergenic 35.0%
! CGAAAAGGTGCAAAGAAAAA+TGG - chr1.2:70693165-70693184 MS.gene60682:intron 35.0%
!! AGAGCATGAACTTCACTTTT+TGG + chr1.2:70693035-70693054 None:intergenic 35.0%
!! TTTTGACTAATTCCACTGAC+AGG + chr1.2:70691553-70691572 None:intergenic 35.0%
!!! TATTTGGTTGTGGTTTTGGA+TGG + chr1.2:70692353-70692372 None:intergenic 35.0%
AACTTCTTCTTCCACAGCTT+CGG - chr1.2:70692557-70692576 MS.gene60682:intron 40.0%
AAGGAGAAACGAATGTGGTA+GGG + chr1.2:70692461-70692480 None:intergenic 40.0%
AAGTCTTAAGGGTCCACTTT+GGG - chr1.2:70691339-70691358 MS.gene60682:CDS 40.0%
ACAAGTGCTTGCAAAACAAG+AGG - chr1.2:70692647-70692666 MS.gene60682:intron 40.0%
ACCTCCAACTACATTTGAAG+AGG - chr1.2:70693397-70693416 MS.gene60682:CDS 40.0%
ATATAGAGGGATCAAGGAAG+TGG + chr1.2:70692141-70692160 None:intergenic 40.0%
CCTCCAACTACATTTGAAGA+GGG - chr1.2:70693398-70693417 MS.gene60682:CDS 40.0%
CTTGAAGTCAAATGAGACGA+TGG - chr1.2:70693340-70693359 MS.gene60682:intron 40.0%
GAAGAGAAAGGAAGAGAGAT+TGG - chr1.2:70691193-70691212 MS.gene60682:CDS 40.0%
GATAGCATGGAAGAAGATGA+AGG - chr1.2:70693521-70693540 MS.gene60682:CDS 40.0%
GCTGAAATATCTTCCCACAA+TGG + chr1.2:70693113-70693132 None:intergenic 40.0%
GTACCCTCTTCAAATGTAGT+TGG + chr1.2:70693404-70693423 None:intergenic 40.0%
TCAAGAGAGCCAATGTTTCT+TGG + chr1.2:70691282-70691301 None:intergenic 40.0%
TGTAGGAAACTAGCTGAACT+TGG - chr1.2:70692168-70692187 MS.gene60682:CDS 40.0%
! ATCAATATTAACGGTGGTGG+AGG - chr1.2:70691241-70691260 MS.gene60682:CDS 40.0%
! GGCCCATTTTCTTGATACTT+TGG + chr1.2:70693083-70693102 None:intergenic 40.0%
! TAGAAGCTCAAAGAAGTGCA+AGG - chr1.2:70692197-70692216 MS.gene60682:CDS 40.0%
! TTGTTGTTAGGTTGTGGTGA+AGG + chr1.2:70692480-70692499 None:intergenic 40.0%
! TTTTCCACTCCTACCTTCTT+TGG + chr1.2:70692259-70692278 None:intergenic 40.0%
!! GGTTACAAAAGTGGTAATGG+TGG - chr1.2:70692984-70693003 MS.gene60682:intron 40.0%
!! TACATTTGAAGAGGGTACTG+TGG - chr1.2:70693406-70693425 MS.gene60682:CDS 40.0%
!! TGAGAATATTGTGGCGGATA+CGG - chr1.2:70691150-70691169 MS.gene60682:CDS 40.0%
!!! TTGTGGTTTTGGATGGTAAG+AGG + chr1.2:70692346-70692365 None:intergenic 40.0%
AACGAATGTGGTAGGGTTTG+TGG + chr1.2:70692454-70692473 None:intergenic 45.0%
AATGTGGTAGGGTTTGTGGA+TGG + chr1.2:70692450-70692469 None:intergenic 45.0%
AGAACCAAAGAAGGTAGGAG+TGG - chr1.2:70692252-70692271 MS.gene60682:CDS 45.0%
AGAGAGCCAATGTTTCTTGG+CGG + chr1.2:70691279-70691298 None:intergenic 45.0%
AGCATGGAAGAAGATGAAGG+AGG - chr1.2:70693524-70693543 MS.gene60682:CDS 45.0%
ATCCGCCACAATATTCTCAC+CGG + chr1.2:70691149-70691168 None:intergenic 45.0%
CAAGTCTTAAGGGTCCACTT+TGG - chr1.2:70691338-70691357 MS.gene60682:CDS 45.0%
CCCTCTTCAAATGTAGTTGG+AGG + chr1.2:70693401-70693420 None:intergenic 45.0%
CTTGAAACCTCTTCCCAAAG+TGG + chr1.2:70691355-70691374 None:intergenic 45.0%
GAAGGAGAAACGAATGTGGT+AGG + chr1.2:70692462-70692481 None:intergenic 45.0%
GAGAAAGGAAGAGAGATTGG+TGG - chr1.2:70691196-70691215 MS.gene60682:CDS 45.0%
GAGCTGCTGATGATCTTTCT+TGG + chr1.2:70692791-70692810 None:intergenic 45.0%
GGTAGGAGTGGAAAATCAGA+AGG - chr1.2:70692264-70692283 MS.gene60682:CDS 45.0%
TCAGCTGCAATGAAGAGACA+AGG - chr1.2:70693128-70693147 MS.gene60682:intron 45.0%
TGGAACCTATTTCCTGTCAG+TGG - chr1.2:70691538-70691557 MS.gene60682:CDS 45.0%
TGGTGAAGGAGAAACGAATG+TGG + chr1.2:70692466-70692485 None:intergenic 45.0%
TTGCTCCGGTGAGAATATTG+TGG - chr1.2:70691141-70691160 MS.gene60682:CDS 45.0%
! CACTTTGGGAAGAGGTTTCA+AGG - chr1.2:70691353-70691372 MS.gene60682:CDS 45.0%
! GTGGCTGTCCAGACAATTTT+TGG + chr1.2:70692842-70692861 None:intergenic 45.0%
!! TTAACGGTGGTGGAGGAAAT+AGG - chr1.2:70691248-70691267 MS.gene60682:CDS 45.0%
!!! TCACTTTTTGGCCACCTTGA+TGG + chr1.2:70693023-70693042 None:intergenic 45.0%
AAGTGGAGGAGACAGAAAGG+AGG + chr1.2:70692124-70692143 None:intergenic 50.0%
AGAGCCAATGTTTCTTGGCG+GGG + chr1.2:70691277-70691296 None:intergenic 50.0%
AGGAAGTGGAGGAGACAGAA+AGG + chr1.2:70692127-70692146 None:intergenic 50.0%
AGGGTTTGTGGATGGAACAG+TGG + chr1.2:70692442-70692461 None:intergenic 50.0%
CTCCGGTGAGAATATTGTGG+CGG - chr1.2:70691144-70691163 MS.gene60682:CDS 50.0%
CTTCTCCTTCTCCATCAAGG+TGG - chr1.2:70693009-70693028 MS.gene60682:intron 50.0%
GAGAGCCAATGTTTCTTGGC+GGG + chr1.2:70691278-70691297 None:intergenic 50.0%
GTGCTTCTCCTTCTCCATCA+AGG - chr1.2:70693006-70693025 MS.gene60682:intron 50.0%
TAGAGGGATCAAGGAAGTGG+AGG + chr1.2:70692138-70692157 None:intergenic 50.0%
TGAGACGATGGAGCCATTGA+TGG - chr1.2:70693352-70693371 MS.gene60682:intron 50.0%
TGGTGCAGCCAGAACAACAA+TGG - chr1.2:70693372-70693391 MS.gene60682:CDS 50.0%
TTTGGCCACCTTGATGGAGA+AGG + chr1.2:70693017-70693036 None:intergenic 50.0%
! GGAGGTCTCCATTGTTGTTC+TGG + chr1.2:70693383-70693402 None:intergenic 50.0%
! TAAGGGTCCACTTTGGGAAG+AGG - chr1.2:70691345-70691364 MS.gene60682:CDS 50.0%
! TCTTCCCACAATGGCGCTTT+TGG + chr1.2:70693104-70693123 None:intergenic 50.0%
! TGTTCTGGCTGCACCATCAA+TGG + chr1.2:70693368-70693387 None:intergenic 50.0%
!! AGGAAGAGAGATTGGTGGTG+AGG - chr1.2:70691201-70691220 MS.gene60682:CDS 50.0%
CAAGTCTTCGTCCGAAGCTG+TGG + chr1.2:70692571-70692590 None:intergenic 55.0%
CACAGCTTCGGACGAAGACT+TGG - chr1.2:70692569-70692588 MS.gene60682:intron 55.0%
GATGCCAGAGCTGTCTGACA+CGG + chr1.2:70692897-70692916 None:intergenic 55.0%
!! ACGGTGGTGGAGGAAATAGG+TGG - chr1.2:70691251-70691270 MS.gene60682:CDS 55.0%
!! ATGGGCCAAAAGCGCCATTG+TGG - chr1.2:70693096-70693115 MS.gene60682:intron 55.0%
!! TGGGCCAAAAGCGCCATTGT+GGG - chr1.2:70693097-70693116 MS.gene60682:intron 55.0%
GATACGGCGCAAAACGGAGG+CGG - chr1.2:70691166-70691185 MS.gene60682:CDS 60.0%
GTCTCCGTGTCAGACAGCTC+TGG - chr1.2:70692890-70692909 MS.gene60682:intron 60.0%
GTCTGACACGGAGACAGCTC+TGG + chr1.2:70692885-70692904 None:intergenic 60.0%
GTGGCCCCGCCAAGAAACAT+TGG - chr1.2:70691270-70691289 MS.gene60682:CDS 60.0%
!! GTGGCGGATACGGCGCAAAA+CGG - chr1.2:70691160-70691179 MS.gene60682:CDS 60.0%
GCGGATACGGCGCAAAACGG+AGG - chr1.2:70691163-70691182 MS.gene60682:CDS 65.0%
GGAGGCGGCGGTGAAGAGAA+AGG - chr1.2:70691181-70691200 MS.gene60682:CDS 65.0%
! CACGGAGACAGCTCTGGTGC+TGG + chr1.2:70692879-70692898 None:intergenic 65.0%
! GGAGACAGCTCTGGTGCTGG+CGG + chr1.2:70692876-70692895 None:intergenic 65.0%
!! TCTGGTGCTGGCGGTGGTTG+TGG + chr1.2:70692867-70692886 None:intergenic 65.0%
ACGGCGCAAAACGGAGGCGG+CGG - chr1.2:70691169-70691188 MS.gene60682:CDS 70.0%
GCTGGCGGTGGTTGTGGTGG+TGG + chr1.2:70692861-70692880 None:intergenic 70.0%
!! GACAGCTCTGGTGCTGGCGG+TGG + chr1.2:70692873-70692892 None:intergenic 70.0%
!! GGTGCTGGCGGTGGTTGTGG+TGG + chr1.2:70692864-70692883 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 70691130 70693610 70691130 ID=MS.gene60682
chr1.2 mRNA 70691130 70693610 70691130 ID=MS.gene60682.t1;Parent=MS.gene60682
chr1.2 exon 70693366 70693610 70693366 ID=MS.gene60682.t1.exon1;Parent=MS.gene60682.t1
chr1.2 CDS 70693366 70693610 70693366 ID=cds.MS.gene60682.t1;Parent=MS.gene60682.t1
chr1.2 exon 70691130 70692567 70691130 ID=MS.gene60682.t1.exon2;Parent=MS.gene60682.t1
chr1.2 CDS 70691130 70692567 70691130 ID=cds.MS.gene60682.t1;Parent=MS.gene60682.t1
Gene Sequence

>MS.gene60682

ATGATGGAGACTTGCTCCGGTGAGAATATTGTGGCGGATACGGCGCAAAACGGAGGCGGCGGTGAAGAGAAAGGAAGAGAGATTGGTGGTGAGGAAGATGATAAGATGAATATCAATATTAACGGTGGTGGAGGAAATAGGTGGCCCCGCCAAGAAACATTGGCTCTCTTGAAGATAAGATCAGATATGGATGGTGTTTTTCGTGATTCAAGTCTTAAGGGTCCACTTTGGGAAGAGGTTTCAAGGTATACTCAATTCAATATTTATATATCTATGTCTTTTCTTTTTGTTTTCTTCTTCTTCTTCTTCATCTTTTTCTATATACCTTTCATTTTCTTGTTTATAAGCTATATCATGCATGAAGAAGATCTGAATTTAGATATGTATCCAAATTAACCAATCAGTTTTTGGAACCTATTTCCTGTCAGTGGAATTAGTCAAAAAAAGCTCTTTGATTGGAATCATTTTTTTTTCCTTCTGAAAAGGCATATATCTTATTCTCAAGTGTATGAGGCAATAATTCAGTTATCTTAGATTTTGAGTCATAAGATAATTTATGTCATTTGAATCATCTAAGTGAAATTCTTCTATCTGAATCTTGCTGCATGTTGAACTCAATCATAGTTTATAATTTACTTCTCCATATATAATTTTATTAACCAATTAAATTACTTAAGTTTAATGTTGAGTTCATCTTCTTCAACTTTTTTTAGGTTAATTTCATCCTTCCATCTAATTTTTATTACTTTGTGTGTTTCTTATTTATTGGTCACTCTTTTAGGTATGAACTACCTAGCTTCATGTGTTTATTTTTTTTTTTTTGGTGTAACATGTGTTTTAATTTAGTACTATATACTTTGTATTTTTCCTCATCACAAATTTTTACTTTTTTTACGTGAAAGGAGTCTAGTTTTTTCTTTTCCTTTTTCAATGGATATACTAACTATATATGATTACAAAATTCAATCATCATTATCAATATTAATTATCACCTCCTTTCTGTCTCCTCCACTTCCTTGATCCCTCTATATATATACATGTAGGAAACTAGCTGAACTTGGCTATCATAGAAGCTCAAAGAAGTGCAAGGAGAAATTTGAAAATGTATACAAGTATCACAAAAGAACCAAAGAAGGTAGGAGTGGAAAATCAGAAGGAAAAACCTATAGATTTTTTGATCAATTACAAGCACTTGAAAAACAACTCACATTCTCCTCTTACCATCCAAAACCACAACCAAATAATAACACACTCTCATCACCAACAATAAATCCCAATGAAACCACACAAACAATATCATATGTCACAACCACTGTTCCATCCACAAACCCTACCACATTCGTTTCTCCTTCACCACAACCTAACAACAACAATGTTCCAAATTCTTTGCCAAACATGAACAACCTATTCTCAACCACAACAACTTCAACTTCTTCTTCCACAGCTTCGGACGAAGACTTGGAAGAGAAGTATAGAAAGAAGAGAAAATGGAAGGACTATTTCAGGAGACTCACAAGACAAGTGCTTGCAAAACAAGAGGAAATGCAAAAGAAGTTTCTTGAGGCTATAGACAAACGTGAAAAAGAACACATTGCTCAACAAGATGCTTTGAGAATTCAAGAAATGGATAGAATCAATAAAGAACACGAACTTCTTATCCAAGAAAGATCATCAGCAGCTCAAAAAAATGCAGCTGTTATTGCATTTCTCCAAAAATTGTCTGGACAGCCACCACCACAACCACCGCCAGCACCAGAGCTGTCTCCGTGTCAGACAGCTCTGGCATCACAAGTTCAAACACAGCAACTAGTGATACCAAACAACAACATTGTTGAATTTCAAAATATGAATAATGGTTACAAAAGTGGTAATGGTGGTGCTTCTCCTTCTCCATCAAGGTGGCCAAAAAGTGAAGTTCATGCTCTAATAAGGATAAGAACAAGTCTTGAACCAAAGTATCAAGAAAATGGGCCAAAAGCGCCATTGTGGGAAGATATTTCAGCTGCAATGAAGAGACAAGGATACAATAGGAATGCGAAAAGGTGCAAAGAAAAATGGGAGAATATCAACAAATACTACAAGAAAATGAAGGAAAGTAATAAGCAAAGGCGTGATGATAGTAAGACATGTCCTTATTTTAATGAACTTGAAGCTATTTACAAAGAAAAGAACAAAACACAGAACCTTTTTGGTTCTAATTCGTTTCATAGCTTGAAGTCAAATGAGACGATGGAGCCATTGATGGTGCAGCCAGAACAACAATGGAGACCTCCAACTACATTTGAAGAGGGTACTGTGGTGAAGAAGAATGTTGATGAAGCAAAAGAAGAAGATGACAATGGTGATGTTGATGATGATGAAGATGAAAACATGGATGAATATGAAGATGGAGATAGCATGGAAGAAGATGAAGGAGGTGTTAGTAACCGTTATGAAGTTGTGACAACAAATAAACTTTCATCAATGGACACAGTTGTATGA
Protein sequence

>MS.gene60682.t1

MMETCSGENIVADTAQNGGGGEEKGREIGGEEDDKMNININGGGGNRWPRQETLALLKIRSDMDGVFRDSSLKGPLWEEVSRKLAELGYHRSSKKCKEKFENVYKYHKRTKEGRSGKSEGKTYRFFDQLQALEKQLTFSSYHPKPQPNNNTLSSPTINPNETTQTISYVTTTVPSTNPTTFVSPSPQPNNNNVPNSLPNMNNLFSTTTTSTSSSTASDEDLEEKYRKKRKWKDYFRRLTRQVLAKQEEMQKKFLEAIDKREKEHIAQQDALRIQEMDRINKEHELLIQERSSAAQKNAAVIAFLQKLSGQPPPQPPPAPELSPCQTALASQVQTQQLVIPNNNIVEFQNMNNGYKSGNGGASPSPSRWPKSEVHALIRIRTSLEPKYQENGPKAPLWEDISAAMKRQGYNRNAKRCKEKWENINKYYKKMKESNKQRRDDSKTCPYFNELEAIYKEKNKTQNLFGSNSFHSLKSNETMEPLMVQPEQQWRPPTTFEEGTVVKKNVDEAKEEDDNGDVDDDEDENMDEYEDGDSMEEDEGGVSNRYEVVTTNKLSSMDTVV