AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60697

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60697.t1 MTR_1g098450 46.526 331 113 10 8 321 7 290 2.36e-82 252
MS.gene60697.t1 MTR_6g043890 29.195 298 181 11 13 301 15 291 4.61e-22 95.1
MS.gene60697.t1 MTR_7g050560 27.922 308 189 10 13 317 13 290 3.20e-19 87.0
MS.gene60697.t1 MTR_5g044670 26.019 319 199 13 13 321 16 307 4.04e-19 86.7
MS.gene60697.t1 MTR_4g119990 27.070 314 210 12 13 320 15 315 2.38e-18 85.1
MS.gene60697.t1 MTR_7g050590 28.378 296 191 9 27 317 1 280 2.98e-18 84.0
MS.gene60697.t1 MTR_3g098790 28.527 319 182 14 13 317 13 299 1.23e-17 82.4
MS.gene60697.t1 MTR_1g108780 27.097 310 176 13 31 321 16 294 1.44e-17 83.2
MS.gene60697.t1 MTR_8g103940 25.786 318 200 9 12 317 38 331 2.12e-17 83.2
MS.gene60697.t1 MTR_1g035460 25.566 309 190 9 27 317 1 287 6.01e-15 75.5
MS.gene60697.t1 MTR_7g050580 25.962 312 190 11 13 317 13 290 2.33e-14 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60697.t1 AT3G18990 23.546 361 185 11 13 317 11 336 2.85e-16 79.0
MS.gene60697.t1 AT3G18990 23.546 361 185 11 13 317 11 336 4.43e-16 78.6

Find 64 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCATTGAGTCGTGAATTT+TGG 0.100356 1.2:-70918700 MS.gene60697:CDS
TAAATTAATTAATAGGTAAA+AGG 0.164286 1.2:-70918183 MS.gene60697:intron
CTCCTCCTAACAATGGATTT+TGG 0.211812 1.2:-70917778 MS.gene60697:CDS
CTTGCCAGTTAGATCAAATA+TGG 0.212339 1.2:+70918481 None:intergenic
GATCTAACTGGCAAGGAAAT+TGG 0.214464 1.2:-70918473 MS.gene60697:CDS
GAATGAAAGTATTGTTATTC+TGG 0.216903 1.2:+70918050 None:intergenic
TGGGGATCCCCTGGTGTTAT+AGG 0.270367 1.2:+70918153 None:intergenic
CCTATAGGTCTTGTGCTTCC+TGG 0.297366 1.2:-70918662 MS.gene60697:CDS
GTTTCCATATTTGATCTAAC+TGG 0.319236 1.2:-70918485 MS.gene60697:CDS
GCGGACAATAAATTGAAAAT+AGG 0.327390 1.2:-70917683 MS.gene60697:CDS
CCACAGTTTCATTAATAGTT+GGG 0.355834 1.2:+70918134 None:intergenic
TGCTTCCTGGTAATTGTATA+TGG 0.364093 1.2:-70918649 MS.gene60697:CDS
AGATGCCAAAATCCATTGTT+AGG 0.365217 1.2:+70917773 None:intergenic
TCTACGTTTAATATCTTCTT+CGG 0.367333 1.2:+70918108 None:intergenic
CTCATGTGATATTAAACAAT+TGG 0.396587 1.2:-70918598 MS.gene60697:CDS
GATGATATTTCTAGCCCTAT+AGG 0.399445 1.2:-70918677 MS.gene60697:CDS
ACCACAGTTTCATTAATAGT+TGG 0.399671 1.2:+70918133 None:intergenic
GGGGATCCCCTGGTGTTATA+GGG 0.400896 1.2:+70918154 None:intergenic
CCAGGAAGCACAAGACCTAT+AGG 0.409533 1.2:+70918662 None:intergenic
AAATTAATTAATAGGTAAAA+GGG 0.423358 1.2:-70918182 MS.gene60697:intron
TCATGAGGCTTTGTCATTTG+AGG 0.430259 1.2:+70918317 None:intergenic
CTGCCTTCGAAATGACGATT+TGG 0.433035 1.2:-70918900 MS.gene60697:intron
ACGTTTCTTTGAATGTATGA+TGG 0.444285 1.2:+70918016 None:intergenic
ATTGTATATGGCGACTGCAC+TGG 0.452158 1.2:-70918637 MS.gene60697:CDS
CTAAAGATTAATACTTGTAA+TGG 0.454684 1.2:-70917746 MS.gene60697:CDS
CAGGAAGCACAAGACCTATA+GGG 0.460045 1.2:+70918663 None:intergenic
CCTCATGATGATATTGCAAA+AGG 0.461954 1.2:-70918302 MS.gene60697:intron
ATGTGTTGTTAAGAAGAAGT+TGG 0.468642 1.2:+70917844 None:intergenic
GAGTTGATCCACTGCCTGTA+GGG 0.472070 1.2:+70918342 None:intergenic
ATTAATAGTTGGGGATCCCC+TGG 0.473898 1.2:+70918144 None:intergenic
TAAGAAGAAGTTGGGTGGTA+AGG 0.474877 1.2:+70917853 None:intergenic
TGTGTTGTTAAGAAGAAGTT+GGG 0.475145 1.2:+70917845 None:intergenic
GAGCTACTGACTCCGTCAAC+CGG 0.475917 1.2:-70917644 MS.gene60697:CDS
CGTTTCTTTGAATGTATGAT+GGG 0.486631 1.2:+70918017 None:intergenic
CATATTTGATCTAACTGGCA+AGG 0.494738 1.2:-70918480 MS.gene60697:CDS
CACAGTTTCATTAATAGTTG+GGG 0.500152 1.2:+70918135 None:intergenic
CTTAACAACACATCAACTAA+AGG 0.504137 1.2:-70917834 MS.gene60697:CDS
GGAGTTGATCCACTGCCTGT+AGG 0.504208 1.2:+70918341 None:intergenic
ATTCTGGAAAACTGAAGCCT+TGG 0.521765 1.2:+70918066 None:intergenic
AAAACGAACCCTACAGGCAG+TGG 0.523906 1.2:-70918350 MS.gene60697:CDS
TGAGGCTTTGTCATTTGAGG+TGG 0.525085 1.2:+70918320 None:intergenic
TAAACGTAGATCTCGTGCAT+TGG 0.536634 1.2:-70918095 MS.gene60697:CDS
AACTTCAAACAAAACATCTC+CGG 0.543526 1.2:+70917625 None:intergenic
AAAATGAAAACGAACCCTAC+AGG 0.548228 1.2:-70918356 MS.gene60697:CDS
ATACATTCAAAGAAACGTGC+TGG 0.559290 1.2:-70918011 MS.gene60697:intron
AACAAAACATCTCCGGTTGA+CGG 0.564670 1.2:+70917632 None:intergenic
GTATGACCATGTCGTACTGC+AGG 0.568497 1.2:+70918398 None:intergenic
TTACCAAATCGTCATTTCGA+AGG 0.572120 1.2:+70918897 None:intergenic
GGGAAGTTCCCTATAACACC+AGG 0.577389 1.2:-70918162 MS.gene60697:CDS
TAAAAGAAGGAGGAGAACAC+GGG 0.577526 1.2:+70918446 None:intergenic
CCCAACTATTAATGAAACTG+TGG 0.601222 1.2:-70918134 MS.gene60697:CDS
AACACATCAACTAAAGGAGA+AGG 0.602007 1.2:-70917828 MS.gene60697:CDS
ATAAAAGAAGGAGGAGAACA+CGG 0.602898 1.2:+70918445 None:intergenic
TGCCAAAATCCATTGTTAGG+AGG 0.618710 1.2:+70917776 None:intergenic
AGTCGCCATATACAATTACC+AGG 0.628511 1.2:+70918644 None:intergenic
AATTGGAAGAGATTTGCTGA+AGG 0.630721 1.2:-70918581 MS.gene60697:CDS
TCGTGCATTGGAGAATGCCA+AGG 0.636441 1.2:-70918083 MS.gene60697:CDS
TCAAAACCTGCAGTACGACA+TGG 0.647736 1.2:-70918404 MS.gene60697:CDS
TTTCCATGGATAAAAGAAGG+AGG 0.655430 1.2:+70918436 None:intergenic
GGAAGTTCCCTATAACACCA+GGG 0.689352 1.2:-70918161 MS.gene60697:CDS
GAAGTTCCCTATAACACCAG+GGG 0.708966 1.2:-70918160 MS.gene60697:CDS
TTTCTTGGGAGGATGAAGAG+GGG 0.717447 1.2:+70918376 None:intergenic
GTTGTTAAGAAGAAGTTGGG+TGG 0.718192 1.2:+70917848 None:intergenic
AAAAGAAGGAGGAGAACACG+GGG 0.769991 1.2:+70918447 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAATTAAATTAATTAAT+AGG - chr1.2:70918367-70918386 MS.gene60697:CDS 0.0%
!! AAATTAATTAATAGGTAAAA+GGG - chr1.2:70918375-70918394 MS.gene60697:CDS 10.0%
!! TAAATTAATTAATAGGTAAA+AGG - chr1.2:70918374-70918393 MS.gene60697:CDS 10.0%
!!! ATTTAATTTAATTTGAGACA+CGG + chr1.2:70918359-70918378 None:intergenic 15.0%
!!! TTTGTTTGTTATAATAATGT+CGG - chr1.2:70917778-70917797 MS.gene60697:CDS 15.0%
!! CTAAAGATTAATACTTGTAA+TGG - chr1.2:70918811-70918830 MS.gene60697:intron 20.0%
!!! CAAATAATATCTTTTAACGA+TGG - chr1.2:70918835-70918854 MS.gene60697:intron 20.0%
!!! GTTTTTGCTTTATATATTTC+TGG - chr1.2:70918572-70918591 MS.gene60697:CDS 20.0%
! AAAAATGAATCACACGAATA+TGG + chr1.2:70917739-70917758 None:intergenic 25.0%
! ATAACAAACAAACAGTAGTA+AGG + chr1.2:70917770-70917789 None:intergenic 25.0%
! CTCATGTGATATTAAACAAT+TGG - chr1.2:70917959-70917978 MS.gene60697:intron 25.0%
! TAACAAACAAACAGTAGTAA+GGG + chr1.2:70917769-70917788 None:intergenic 25.0%
! TCTACGTTTAATATCTTCTT+CGG + chr1.2:70918452-70918471 None:intergenic 25.0%
!! ATATCCCATTAGCATTTTTA+GGG - chr1.2:70918050-70918069 MS.gene60697:CDS 25.0%
!! ATCAACAATTTTGCAAAACT+TGG + chr1.2:70917632-70917651 None:intergenic 25.0%
!! TAATATCTTTTAACGATGGT+TGG - chr1.2:70918839-70918858 MS.gene60697:intron 25.0%
!!! GAATGAAAGTATTGTTATTC+TGG + chr1.2:70918510-70918529 None:intergenic 25.0%
!!! TTTGATGTTTTGATTTTCCA+TGG + chr1.2:70918138-70918157 None:intergenic 25.0%
AAAATGAAAACCGTCAAAAC+AGG - chr1.2:70918681-70918700 MS.gene60697:CDS 30.0%
AACTTCAAACAAAACATCTC+CGG + chr1.2:70918935-70918954 None:intergenic 30.0%
AATAGCATGAGCATAGTTTA+TGG + chr1.2:70918311-70918330 None:intergenic 30.0%
ACCACAGTTTCATTAATAGT+TGG + chr1.2:70918427-70918446 None:intergenic 30.0%
ACGTTTCTTTGAATGTATGA+TGG + chr1.2:70918544-70918563 None:intergenic 30.0%
CACAGTTTCATTAATAGTTG+GGG + chr1.2:70918425-70918444 None:intergenic 30.0%
CCACAGTTTCATTAATAGTT+GGG + chr1.2:70918426-70918445 None:intergenic 30.0%
CGTTTCTTTGAATGTATGAT+GGG + chr1.2:70918543-70918562 None:intergenic 30.0%
CTTAACAACACATCAACTAA+AGG - chr1.2:70918723-70918742 MS.gene60697:intron 30.0%
GAAACCCTAAAAATGCTAAT+GGG + chr1.2:70918057-70918076 None:intergenic 30.0%
GCGGACAATAAATTGAAAAT+AGG - chr1.2:70918874-70918893 MS.gene60697:intron 30.0%
GTTTCCATATTTGATCTAAC+TGG - chr1.2:70918072-70918091 MS.gene60697:CDS 30.0%
TGAACAATGAAGATTTAGTG+AGG + chr1.2:70917707-70917726 None:intergenic 30.0%
! ATGTGTTGTTAAGAAGAAGT+TGG + chr1.2:70918716-70918735 None:intergenic 30.0%
! CATATCCCATTAGCATTTTT+AGG - chr1.2:70918049-70918068 MS.gene60697:CDS 30.0%
! GATTTTCCATGGATAAAAGA+AGG + chr1.2:70918127-70918146 None:intergenic 30.0%
! TGTGTTGTTAAGAAGAAGTT+GGG + chr1.2:70918715-70918734 None:intergenic 30.0%
AAAATGAAAACGAACCCTAC+AGG - chr1.2:70918201-70918220 MS.gene60697:intron 35.0%
AACACATCAACTAAAGGAGA+AGG - chr1.2:70918729-70918748 MS.gene60697:intron 35.0%
AAGATTTAGTGAGGCAATCA+AGG + chr1.2:70917698-70917717 None:intergenic 35.0%
AGATGCCAAAATCCATTGTT+AGG + chr1.2:70918787-70918806 None:intergenic 35.0%
ATAAAAGAAGGAGGAGAACA+CGG + chr1.2:70918115-70918134 None:intergenic 35.0%
ATACATTCAAAGAAACGTGC+TGG - chr1.2:70918546-70918565 MS.gene60697:CDS 35.0%
CATATTTGATCTAACTGGCA+AGG - chr1.2:70918077-70918096 MS.gene60697:CDS 35.0%
CCCAACTATTAATGAAACTG+TGG - chr1.2:70918423-70918442 MS.gene60697:CDS 35.0%
CCTCATGATGATATTGCAAA+AGG - chr1.2:70918255-70918274 MS.gene60697:intron 35.0%
CTTGCCAGTTAGATCAAATA+TGG + chr1.2:70918079-70918098 None:intergenic 35.0%
GGAAACCCTAAAAATGCTAA+TGG + chr1.2:70918058-70918077 None:intergenic 35.0%
TGCTTCCTGGTAATTGTATA+TGG - chr1.2:70917908-70917927 MS.gene60697:intron 35.0%
TTACCAAATCGTCATTTCGA+AGG + chr1.2:70917663-70917682 None:intergenic 35.0%
TTTATGGAAGGAAGGAAAGA+AGG + chr1.2:70918295-70918314 None:intergenic 35.0%
TTTCCATGGATAAAAGAAGG+AGG + chr1.2:70918124-70918143 None:intergenic 35.0%
! AGGGTTCGTTTTCATTTTCT+TGG + chr1.2:70918199-70918218 None:intergenic 35.0%
! CCTTTTGCAATATCATCATG+AGG + chr1.2:70918258-70918277 None:intergenic 35.0%
! GGGTTCGTTTTCATTTTCTT+GGG + chr1.2:70918198-70918217 None:intergenic 35.0%
! TGGTTGGAAGAATTTTGTTG+CGG - chr1.2:70918855-70918874 MS.gene60697:intron 35.0%
! TTCGTTTTCATTTTCTTGGG+AGG + chr1.2:70918195-70918214 None:intergenic 35.0%
!! AATTGGAAGAGATTTGCTGA+AGG - chr1.2:70917976-70917995 MS.gene60697:intron 35.0%
!! AGCATTGAGTCGTGAATTTT+GGG - chr1.2:70917858-70917877 MS.gene60697:intron 35.0%
!! GATGATATTTCTAGCCCTAT+AGG - chr1.2:70917880-70917899 MS.gene60697:intron 35.0%
AACAAAACATCTCCGGTTGA+CGG + chr1.2:70918928-70918947 None:intergenic 40.0%
AGTCGCCATATACAATTACC+AGG + chr1.2:70917916-70917935 None:intergenic 40.0%
GAGCATAGTTTATGGAAGGA+AGG + chr1.2:70918303-70918322 None:intergenic 40.0%
GATCTAACTGGCAAGGAAAT+TGG - chr1.2:70918084-70918103 MS.gene60697:CDS 40.0%
GCATGAGCATAGTTTATGGA+AGG + chr1.2:70918307-70918326 None:intergenic 40.0%
GTTGTTAAGAAGAAGTTGGG+TGG + chr1.2:70918712-70918731 None:intergenic 40.0%
TAAAAGAAGGAGGAGAACAC+GGG + chr1.2:70918114-70918133 None:intergenic 40.0%
TAAACGTAGATCTCGTGCAT+TGG - chr1.2:70918462-70918481 MS.gene60697:CDS 40.0%
TAAGAAGAAGTTGGGTGGTA+AGG + chr1.2:70918707-70918726 None:intergenic 40.0%
TGCCAAAATCCATTGTTAGG+AGG + chr1.2:70918784-70918803 None:intergenic 40.0%
! ATTCTGGAAAACTGAAGCCT+TGG + chr1.2:70918494-70918513 None:intergenic 40.0%
! ATTTTCTTGGGAGGATGAAG+AGG + chr1.2:70918186-70918205 None:intergenic 40.0%
! TCATGAGGCTTTGTCATTTG+AGG + chr1.2:70918243-70918262 None:intergenic 40.0%
! TCTCCTCCTTCTTTTATCCA+TGG - chr1.2:70918118-70918137 MS.gene60697:CDS 40.0%
! TTTTCTTGGGAGGATGAAGA+GGG + chr1.2:70918185-70918204 None:intergenic 40.0%
!! CAGCATTGAGTCGTGAATTT+TGG - chr1.2:70917857-70917876 MS.gene60697:CDS 40.0%
!! CTCCTCCTAACAATGGATTT+TGG - chr1.2:70918779-70918798 MS.gene60697:intron 40.0%
!! CTTTTGACTCCTCCTAACAA+TGG - chr1.2:70918772-70918791 MS.gene60697:intron 40.0%
!! GTCAAGCTTAGTTCTGCAAT+TGG - chr1.2:70918638-70918657 MS.gene60697:CDS 40.0%
AAAAGAAGGAGGAGAACACG+GGG + chr1.2:70918113-70918132 None:intergenic 45.0%
ATTAATAGTTGGGGATCCCC+TGG + chr1.2:70918416-70918435 None:intergenic 45.0%
ATTGTATATGGCGACTGCAC+TGG - chr1.2:70917920-70917939 MS.gene60697:intron 45.0%
CTGCCTTCGAAATGACGATT+TGG - chr1.2:70917657-70917676 MS.gene60697:CDS 45.0%
GAAGTTCCCTATAACACCAG+GGG - chr1.2:70918397-70918416 MS.gene60697:CDS 45.0%
GGAAGTTCCCTATAACACCA+GGG - chr1.2:70918396-70918415 MS.gene60697:CDS 45.0%
TCAAAACCTGCAGTACGACA+TGG - chr1.2:70918153-70918172 MS.gene60697:CDS 45.0%
TTAGTGAGGCAATCAAGGAC+TGG + chr1.2:70917693-70917712 None:intergenic 45.0%
TTTCTTGGGAGGATGAAGAG+GGG + chr1.2:70918184-70918203 None:intergenic 45.0%
! CAGGAAGCACAAGACCTATA+GGG + chr1.2:70917897-70917916 None:intergenic 45.0%
! TGAGGCTTTGTCATTTGAGG+TGG + chr1.2:70918240-70918259 None:intergenic 45.0%
AAAACGAACCCTACAGGCAG+TGG - chr1.2:70918207-70918226 MS.gene60697:intron 50.0%
GGGAAGTTCCCTATAACACC+AGG - chr1.2:70918395-70918414 MS.gene60697:CDS 50.0%
GTATGACCATGTCGTACTGC+AGG + chr1.2:70918162-70918181 None:intergenic 50.0%
TCGTGCATTGGAGAATGCCA+AGG - chr1.2:70918474-70918493 MS.gene60697:CDS 50.0%
! CCAGGAAGCACAAGACCTAT+AGG + chr1.2:70917898-70917917 None:intergenic 50.0%
! CCTATAGGTCTTGTGCTTCC+TGG - chr1.2:70917895-70917914 MS.gene60697:intron 50.0%
! GAGTTGATCCACTGCCTGTA+GGG + chr1.2:70918218-70918237 None:intergenic 50.0%
!! GGTGGTAAGGCCTGTTTTGA+CGG + chr1.2:70918694-70918713 None:intergenic 50.0%
! GGAGTTGATCCACTGCCTGT+AGG + chr1.2:70918219-70918238 None:intergenic 55.0%
!! GAGCTACTGACTCCGTCAAC+CGG - chr1.2:70918913-70918932 MS.gene60697:CDS 55.0%
!! GGGGATCCCCTGGTGTTATA+GGG + chr1.2:70918406-70918425 None:intergenic 55.0%
!! TGGGGATCCCCTGGTGTTAT+AGG + chr1.2:70918407-70918426 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 70917598 70918981 70917598 ID=MS.gene60697
chr1.2 mRNA 70917598 70918981 70917598 ID=MS.gene60697.t1;Parent=MS.gene60697
chr1.2 exon 70918901 70918981 70918901 ID=MS.gene60697.t1.exon1;Parent=MS.gene60697.t1
chr1.2 CDS 70918901 70918981 70918901 ID=cds.MS.gene60697.t1;Parent=MS.gene60697.t1
chr1.2 exon 70918303 70918729 70918303 ID=MS.gene60697.t1.exon2;Parent=MS.gene60697.t1
chr1.2 CDS 70918303 70918729 70918303 ID=cds.MS.gene60697.t1;Parent=MS.gene60697.t1
chr1.2 exon 70918012 70918190 70918012 ID=MS.gene60697.t1.exon3;Parent=MS.gene60697.t1
chr1.2 CDS 70918012 70918190 70918012 ID=cds.MS.gene60697.t1;Parent=MS.gene60697.t1
chr1.2 exon 70917598 70917876 70917598 ID=MS.gene60697.t1.exon4;Parent=MS.gene60697.t1
chr1.2 CDS 70917598 70917876 70917598 ID=cds.MS.gene60697.t1;Parent=MS.gene60697.t1
Gene Sequence

>MS.gene60697

ATGATCAAATATTTCACTCATGATCAAGATTCCAAGTTTTGCAAAATTGTTGATGAAGACTGCCTTCGAAATGACGATTTGGTAATTAACATCCAGTCCTTGATTGCCTCACTAAATCTTCATTGTTCATCTTAAAAACCATATTCGTGTGATTCATTTTTTCTTTTACCCTTACTACTGTTTGTTTGTTATAATAATGTCGGTTAATTAATATTGTTGAATGAATGTCATTCTATTCTATACTACTGACAGATGATTGCAGCATTGAGTCGTGAATTTTGGGATGATATTTCTAGCCCTATAGGTCTTGTGCTTCCTGGTAATTGTATATGGCGACTGCACTGGAAACAACATGATGACACTCATGTGATATTAAACAATTGGAAGAGATTTGCTGAAGGTTATTCTTTGAAGCAGAATCAAGTTTTGTTCTTCAAATATGTTGAAAAATCATATCCCATTAGCATTTTTAGGGTTTCCATATTTGATCTAACTGGCAAGGAAATTGGTTACCCCGTGTTCTCCTCCTTCTTTTATCCATGGAAAATCAAAACATCAAAACCTGCAGTACGACATGGTCATACCCCTCTTCATCCTCCCAAGAAAATGAAAACGAACCCTACAGGCAGTGGATCAACTCCACCTCAAATGACAAAGCCTCATGATGATATTGCAAAAGGTACATTTCTTTCTTCCTTCTTTCCTTCCTTCCATAAACTATGCTCATGCTATTAATCATTTTCATTTTTTATTTCATTCCGTGTCTCAAATTAAATTAAATTAATTAATAGGTAAAAGGGAAGTTCCCTATAACACCAGGGGATCCCCAACTATTAATGAAACTGTGGTTTCCGAAGAAGATATTAAACGTAGATCTCGTGCATTGGAGAATGCCAAGGCTTCAGTTTTCCAGAATAACAATACTTTCATTCGTCAAATGCGCCCATCATACATTCAAAGAAACGTGCTGGTAAGTTTTTGCTTTATATATTTCTGGTTGAAAAGTATATGTAAATCAAAATTCATATTATCGAAACTTTGTCAAGCTTAGTTCTGCAATTGGTAATGTTGTGCTATGATAAAAAAATGAAAACCGTCAAAACAGGCCTTACCACCCAACTTCTTCTTAACAACACATCAACTAAAGGAGAAGGAGTATCATGAGTGCGCTGTTCTTTTGACTCCTCCTAACAATGGATTTTGGCATCTCAAGCTAAAGATTAATACTTGTAATGGTCAAATAATATCTTTTAACGATGGTTGGAAGAATTTTGTTGCGGACAATAAATTGAAAATAGGTGATGTATGTCTTTTTGAGCTACTGACTCCGTCAACCGGAGATGTTTTGTTTGAAGTTGTTATTTTCCGTGCAAGAAAACAATGA
Protein sequence

>MS.gene60697.t1

MIKYFTHDQDSKFCKIVDEDCLRNDDLMIAALSREFWDDISSPIGLVLPGNCIWRLHWKQHDDTHVILNNWKRFAEGYSLKQNQVLFFKYVEKSYPISIFRVSIFDLTGKEIGYPVFSSFFYPWKIKTSKPAVRHGHTPLHPPKKMKTNPTGSGSTPPQMTKPHDDIAKGKREVPYNTRGSPTINETVVSEEDIKRRSRALENAKASVFQNNNTFIRQMRPSYIQRNVLALPPNFFLTTHQLKEKEYHECAVLLTPPNNGFWHLKLKINTCNGQIISFNDGWKNFVADNKLKIGDVCLFELLTPSTGDVLFEVVIFRARKQ