AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60862

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60862.t1 MTR_1g093080 97.090 378 11 0 1 378 1 378 0.0 764
MS.gene60862.t1 MTR_1g093080 97.318 261 7 0 1 261 1 261 0.0 530
MS.gene60862.t1 MTR_1g093080 97.034 236 7 0 1 236 1 236 3.85e-172 480
MS.gene60862.t1 MTR_5g054300 57.368 380 126 14 1 374 10 359 1.25e-125 366
MS.gene60862.t1 MTR_4g113140 35.404 322 144 7 2 294 29 315 1.97e-48 169
MS.gene60862.t1 MTR_4g086835 34.930 355 146 11 10 309 44 368 1.15e-42 154
MS.gene60862.t1 MTR_4g086835 34.930 355 145 12 10 309 44 367 6.51e-41 149
MS.gene60862.t1 MTR_5g017980 33.333 330 143 12 2 324 35 294 1.26e-33 127
MS.gene60862.t1 MTR_5g017980 33.435 329 142 12 2 324 35 292 3.17e-33 126
MS.gene60862.t1 MTR_5g017980 33.636 330 141 13 2 324 35 293 6.87e-32 123
MS.gene60862.t1 MTR_7g115530 34.437 151 89 3 162 304 161 309 1.28e-15 77.8
MS.gene60862.t1 MTR_2g027860 56.061 66 29 0 211 276 49 114 1.54e-15 77.4
MS.gene60862.t1 MTR_2g034960 37.008 127 57 4 210 323 210 326 3.15e-15 77.4
MS.gene60862.t1 MTR_2g450070 38.462 117 63 4 209 323 211 320 8.72e-15 76.3
MS.gene60862.t1 MTR_4g121020 53.521 71 32 1 210 280 110 179 9.24e-15 76.3
MS.gene60862.t1 MTR_4g121020 53.521 71 32 1 210 280 214 283 9.49e-15 76.3
MS.gene60862.t1 MTR_2g450070 38.462 117 63 4 209 323 211 320 9.55e-15 76.3
MS.gene60862.t1 MTR_4g121020 53.521 71 32 1 210 280 214 283 9.57e-15 76.3
MS.gene60862.t1 MTR_7g088070 54.167 72 31 1 211 280 50 121 1.17e-14 73.9
MS.gene60862.t1 MTR_7g088070 55.385 65 29 0 211 275 50 114 1.54e-14 73.6
MS.gene60862.t1 MTR_4g131580 53.968 63 28 1 209 271 198 259 2.36e-14 74.7
MS.gene60862.t1 MTR_1g032570 48.611 72 36 1 200 271 120 190 3.27e-14 74.3
MS.gene60862.t1 MTR_1g032570 48.611 72 36 1 200 271 189 259 4.49e-14 73.9
MS.gene60862.t1 MTR_3g102600 43.038 79 43 2 215 292 87 164 4.97e-14 70.1
MS.gene60862.t1 MTR_3g106220 45.679 81 42 2 204 284 191 269 5.52e-14 73.9
MS.gene60862.t1 MTR_7g115530 37.815 119 66 2 162 272 161 279 7.23e-14 72.0
MS.gene60862.t1 MTR_7g117705 48.611 72 36 1 209 280 189 259 8.69e-14 72.8
MS.gene60862.t1 MTR_5g014040 51.515 66 31 1 204 269 304 368 9.46e-14 72.8
MS.gene60862.t1 MTR_7g115530 37.815 119 66 2 162 272 161 279 1.12e-13 71.6
MS.gene60862.t1 MTR_3g102600 43.038 79 43 2 215 292 136 213 1.19e-13 70.1
MS.gene60862.t1 MTR_5g041350 61.818 55 21 0 211 265 15 69 1.28e-13 71.6
MS.gene60862.t1 MTR_4g131570 50.000 66 32 1 206 271 198 262 1.35e-13 72.4
MS.gene60862.t1 MTR_7g115530 54.839 62 28 0 211 272 218 279 2.75e-13 71.2
MS.gene60862.t1 MTR_4g098870 41.489 94 51 2 178 268 274 366 2.94e-13 71.6
MS.gene60862.t1 MTR_1g013170 52.381 63 29 1 209 271 189 250 3.18e-13 71.2
MS.gene60862.t1 MTR_4g098870 41.489 94 51 2 178 268 294 386 3.21e-13 71.2
MS.gene60862.t1 MTR_4g098870 41.489 94 51 2 178 268 274 366 3.29e-13 71.2
MS.gene60862.t1 MTR_4g098870 41.489 94 51 2 178 268 313 405 3.31e-13 71.2
MS.gene60862.t1 MTR_7g098250 43.902 82 44 1 211 292 262 341 3.55e-13 70.9
MS.gene60862.t1 MTR_4g098870 41.489 94 51 2 178 268 294 386 3.66e-13 71.2
MS.gene60862.t1 MTR_2g027800 60.000 55 22 0 211 265 39 93 5.50e-13 69.3
MS.gene60862.t1 MTR_1g080330 50.794 63 31 0 211 273 175 237 5.74e-13 68.9
MS.gene60862.t1 MTR_2g027800 60.000 55 22 0 211 265 39 93 8.05e-13 68.9
MS.gene60862.t1 MTR_1g080330 50.794 63 31 0 211 273 175 237 9.61e-13 69.3
MS.gene60862.t1 MTR_4g064730 43.243 74 41 1 207 280 127 199 1.14e-12 68.6
MS.gene60862.t1 MTR_6g444980 60.000 55 22 0 211 265 35 89 1.32e-12 68.9
MS.gene60862.t1 MTR_6g444980 60.000 55 22 0 211 265 35 89 1.49e-12 68.6
MS.gene60862.t1 MTR_6g032990 48.529 68 35 0 211 278 42 109 1.53e-12 68.9
MS.gene60862.t1 MTR_4g081710 58.182 55 23 0 211 265 17 71 1.62e-12 68.2
MS.gene60862.t1 MTR_3g102590 43.590 78 41 2 209 284 141 217 1.63e-12 67.0
MS.gene60862.t1 MTR_2g086450 56.364 55 24 0 211 265 45 99 1.73e-12 68.6
MS.gene60862.t1 MTR_1g053835 51.667 60 29 0 211 270 258 317 1.78e-12 68.6
MS.gene60862.t1 MTR_7g089010 40.244 82 45 2 188 269 71 148 1.81e-12 67.8
MS.gene60862.t1 MTR_1g053830 49.057 53 27 0 213 265 20 72 2.09e-12 63.2
MS.gene60862.t1 MTR_4g078810 37.895 95 46 2 209 303 156 237 3.70e-12 67.8
MS.gene60862.t1 MTR_4g021790 48.571 70 35 1 200 269 157 225 4.06e-12 66.6
MS.gene60862.t1 MTR_1g090670 58.182 55 23 0 211 265 44 98 4.99e-12 66.2
MS.gene60862.t1 MTR_2g090675 45.070 71 38 1 209 279 159 228 7.41e-12 66.6
MS.gene60862.t1 MTR_8g079940 46.479 71 37 1 210 280 208 277 7.45e-12 67.0
MS.gene60862.t1 MTR_5g014040 47.887 71 31 2 204 269 264 333 7.73e-12 67.0
MS.gene60862.t1 MTR_4g131600 46.970 66 33 2 204 268 193 257 8.83e-12 67.0
MS.gene60862.t1 MTR_5g014040 47.887 71 31 2 204 269 304 373 8.87e-12 67.0
MS.gene60862.t1 MTR_2g023580 55.769 52 23 0 213 264 57 108 1.27e-11 64.3
MS.gene60862.t1 MTR_5g027440 50.820 61 28 1 211 269 25 85 1.88e-11 64.7
MS.gene60862.t1 MTR_7g093030 46.269 67 36 0 211 277 212 278 1.99e-11 64.7
MS.gene60862.t1 MTR_3g086100 40.698 86 45 2 200 280 112 196 2.02e-11 65.5
MS.gene60862.t1 MTR_7g115530 37.615 109 60 2 162 262 161 269 2.22e-11 64.3
MS.gene60862.t1 MTR_0450s0040 43.243 74 41 1 210 283 168 240 2.64e-11 64.7
MS.gene60862.t1 MTR_3g086100 40.698 86 45 2 200 280 183 267 3.36e-11 65.1
MS.gene60862.t1 MTR_7g115530 31.852 135 83 3 162 288 161 294 3.56e-11 63.9
MS.gene60862.t1 MTR_7g093030 46.269 67 36 0 211 277 212 278 5.06e-11 63.9
MS.gene60862.t1 MTR_7g026400 49.153 59 29 1 210 268 156 213 5.71e-11 64.3
MS.gene60862.t1 MTR_4g021760 40.506 79 46 1 208 286 166 243 9.64e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60862.t1 AT1G68670 47.929 338 126 12 10 326 16 324 1.56e-79 248
MS.gene60862.t1 AT1G25550 46.386 332 125 11 10 328 20 311 1.64e-77 243
MS.gene60862.t1 AT3G25790 43.869 367 166 8 10 372 20 350 4.57e-75 237
MS.gene60862.t1 AT1G13300 43.207 368 148 12 11 368 20 336 5.54e-71 226
MS.gene60862.t1 AT1G25550 49.231 260 104 8 10 261 20 259 1.13e-66 213
MS.gene60862.t1 AT3G25790 46.457 254 109 4 9 261 19 246 1.69e-56 186
MS.gene60862.t1 AT2G03500 35.802 324 126 9 8 294 36 314 1.78e-48 170
MS.gene60862.t1 AT4G37180 33.935 277 109 8 11 269 48 268 2.99e-33 127
MS.gene60862.t1 AT4G37180 33.574 277 117 8 11 269 48 275 1.38e-30 120
MS.gene60862.t1 AT1G49560 32.542 295 132 9 10 293 30 268 4.51e-30 118
MS.gene60862.t1 AT4G37180 33.086 269 106 8 11 261 48 260 1.37e-28 113
MS.gene60862.t1 AT1G49560 31.939 263 118 8 10 261 30 242 3.63e-23 97.8
MS.gene60862.t1 AT2G01060 57.812 64 27 0 211 274 15 78 2.05e-15 76.3
MS.gene60862.t1 AT3G16857 35.252 139 79 3 199 336 155 283 8.42e-15 76.3
MS.gene60862.t1 AT3G16857 34.752 141 81 3 199 338 225 355 9.75e-15 76.3
MS.gene60862.t1 AT3G16857 34.752 141 81 3 199 338 225 355 1.02e-14 76.3
MS.gene60862.t1 AT5G58080 50.000 66 32 1 204 269 187 251 4.14e-14 74.3
MS.gene60862.t1 AT3G04030 53.846 65 30 0 211 275 45 109 8.97e-14 72.4
MS.gene60862.t1 AT3G04030 53.846 65 30 0 211 275 45 109 1.05e-13 72.4
MS.gene60862.t1 AT2G25180 31.902 163 94 5 209 356 193 353 1.13e-13 72.8
MS.gene60862.t1 AT5G05090 45.570 79 41 2 192 269 61 138 1.76e-13 70.5
MS.gene60862.t1 AT5G06800 53.226 62 29 0 211 272 192 253 2.77e-13 70.1
MS.gene60862.t1 AT5G18240 58.182 55 23 0 211 265 45 99 3.65e-13 70.9
MS.gene60862.t1 AT5G18240 58.182 55 23 0 211 265 45 99 3.65e-13 70.9
MS.gene60862.t1 AT5G18240 58.182 55 23 0 211 265 45 99 4.03e-13 70.5
MS.gene60862.t1 AT5G18240 58.182 55 23 0 211 265 45 99 4.13e-13 70.5
MS.gene60862.t1 AT5G18240 58.182 55 23 0 211 265 45 99 4.13e-13 70.5
MS.gene60862.t1 AT2G01760 44.156 77 42 1 210 286 134 209 4.99e-13 69.7
MS.gene60862.t1 AT5G44190 43.750 80 44 1 200 279 136 214 6.00e-13 69.7
MS.gene60862.t1 AT5G44190 43.750 80 44 1 200 279 136 214 6.87e-13 69.7
MS.gene60862.t1 AT2G01760 46.575 73 38 1 210 282 227 298 6.89e-13 70.1
MS.gene60862.t1 AT2G01760 44.156 77 42 1 210 286 199 274 7.46e-13 69.7
MS.gene60862.t1 AT5G06800 53.226 62 29 0 211 272 192 253 9.94e-13 69.3
MS.gene60862.t1 AT5G06800 53.226 62 29 0 211 272 192 253 1.05e-12 69.3
MS.gene60862.t1 AT3G10760 44.444 81 43 2 190 269 83 162 1.06e-12 68.9
MS.gene60862.t1 AT2G40970 45.070 71 38 1 199 269 93 162 1.10e-12 67.8
MS.gene60862.t1 AT3G46640 33.113 151 92 4 134 276 59 208 1.46e-12 68.6
MS.gene60862.t1 AT3G46640 46.269 67 35 1 210 276 143 208 1.50e-12 68.2
MS.gene60862.t1 AT3G46640 46.269 67 35 1 210 276 143 208 1.50e-12 68.2
MS.gene60862.t1 AT5G45580 44.595 74 41 0 211 284 23 96 1.51e-12 67.8
MS.gene60862.t1 AT1G79430 60.000 55 22 0 211 265 34 88 1.57e-12 68.6
MS.gene60862.t1 AT5G45580 44.595 74 41 0 211 284 7 80 1.58e-12 67.4
MS.gene60862.t1 AT3G04450 51.667 60 29 0 211 270 238 297 1.66e-12 68.2
MS.gene60862.t1 AT5G59570 38.318 107 64 2 163 269 94 198 2.18e-12 67.8
MS.gene60862.t1 AT5G59570 38.318 107 64 2 163 269 94 198 2.18e-12 67.8
MS.gene60862.t1 AT3G24120 58.182 55 23 0 211 265 41 95 2.29e-12 67.4
MS.gene60862.t1 AT5G29000 49.206 63 32 0 211 273 231 293 2.45e-12 67.4
MS.gene60862.t1 AT4G13640 58.182 55 23 0 211 265 37 91 2.58e-12 67.4
MS.gene60862.t1 AT3G24120 58.182 55 23 0 211 265 41 95 2.63e-12 67.4
MS.gene60862.t1 AT4G13640 58.182 55 23 0 211 265 37 91 2.71e-12 67.4
MS.gene60862.t1 AT3G13040 52.459 61 29 0 210 270 240 300 2.99e-12 68.2
MS.gene60862.t1 AT3G13040 52.459 61 29 0 210 270 240 300 2.99e-12 68.2
MS.gene60862.t1 AT3G13040 52.459 61 29 0 210 270 240 300 2.99e-12 68.2
MS.gene60862.t1 AT3G04450 51.667 60 29 0 211 270 238 297 3.19e-12 68.2
MS.gene60862.t1 AT3G04450 51.667 60 29 0 211 270 198 257 3.68e-12 67.8
MS.gene60862.t1 AT3G04450 51.667 60 29 0 211 270 198 257 3.68e-12 67.8
MS.gene60862.t1 AT2G20570 47.945 73 37 1 207 279 149 220 4.19e-12 67.8
MS.gene60862.t1 AT3G04030 52.308 65 30 1 211 275 45 108 5.76e-12 67.0
MS.gene60862.t1 AT5G29000 49.206 63 32 0 211 273 231 293 5.78e-12 67.0
MS.gene60862.t1 AT5G29000 49.206 63 32 0 211 273 188 250 6.12e-12 67.0
MS.gene60862.t1 AT5G29000 49.206 63 32 0 211 273 188 250 6.12e-12 67.0
MS.gene60862.t1 AT4G28610 51.667 60 29 0 211 270 225 284 6.66e-12 67.0
MS.gene60862.t1 AT3G12730 56.364 55 24 0 211 265 23 77 7.55e-12 65.1
MS.gene60862.t1 AT2G42660 52.632 57 27 0 216 272 55 111 1.05e-11 65.1
MS.gene60862.t1 AT2G38300 51.923 52 25 0 213 264 56 107 1.82e-11 65.1
MS.gene60862.t1 AT4G31920 42.105 76 43 1 204 279 176 250 2.32e-11 65.5
MS.gene60862.t1 AT2G06020 50.909 55 27 0 211 265 86 140 2.55e-11 64.3
MS.gene60862.t1 AT1G49190 36.486 74 44 1 196 266 200 273 5.81e-11 63.9
MS.gene60862.t1 AT4G04605 51.923 52 25 0 213 264 16 67 7.78e-11 62.0

Find 77 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTAACTCTATCTTCTTTA+TGG 0.063110 1.4:+73253788 None:intergenic
CACAATGCAGCGTTTCAAAA+TGG 0.179894 1.4:-73254527 None:intergenic
ACAAAAGTGGTTGCACCTTC+TGG 0.247597 1.4:-73252972 MS.gene60862:CDS
CATATCTACAAACCTGTAAA+TGG 0.279375 1.4:+73253379 None:intergenic
TGAAGTCAAAAGCCATTTAC+AGG 0.284068 1.4:-73253391 MS.gene60862:intron
AACGAAAGAGGAAGTTCTTT+AGG 0.297245 1.4:+73254448 None:intergenic
GATGTCTCCAACATTGTTAC+TGG 0.321828 1.4:+73253684 None:intergenic
ATTACCTCTCTGATTTGCTT+TGG 0.356836 1.4:+73253438 None:intergenic
GTCGTTCTTGTTCTAATGCT+TGG 0.361767 1.4:+73254488 None:intergenic
CTTTCGTTATTTGTTGAACT+TGG 0.382926 1.4:+73253098 None:intergenic
GGCTTAGATCTGTTCAGTTA+TGG 0.389861 1.4:-73254047 MS.gene60862:CDS
CCCCACCATACTCTTGTTGT+TGG 0.395076 1.4:+73253024 None:intergenic
GGAATAAACTCTTCTAAAAC+AGG 0.409660 1.4:+73254189 None:intergenic
TTTGTCCAACAACAAGAGTA+TGG 0.414336 1.4:-73253029 MS.gene60862:CDS
ATTATTACCTCTTCAGATGA+AGG 0.417768 1.4:+73254015 None:intergenic
ACAATGCAGCGTTTCAAAAT+GGG 0.423030 1.4:-73254526 MS.gene60862:CDS
GTAGCTTCATCAACAACAAC+AGG 0.429695 1.4:-73253002 MS.gene60862:CDS
TGCTCAAACCTCTTGAACTC+TGG 0.432408 1.4:+73252900 None:intergenic
GCAGCGACGGTGCTGGTCGC+AGG 0.434166 1.4:-73253632 MS.gene60862:CDS
GTTTATTCCAATTAAGAAAA+GGG 0.437351 1.4:-73254175 MS.gene60862:CDS
GTTGCACAAGCGATTCTTGA+AGG 0.440339 1.4:-73253608 MS.gene60862:CDS
AGTTTATTCCAATTAAGAAA+AGG 0.446516 1.4:-73254176 MS.gene60862:CDS
ACTCTATCTTCTTTATGGAA+TGG 0.449526 1.4:+73253793 None:intergenic
GCTTGTCGACAACAATTGTC+TGG 0.459321 1.4:-73254279 MS.gene60862:CDS
CATCAAAAGAGACGAGAAAG+AGG 0.461312 1.4:-73253665 MS.gene60862:CDS
TTGAAGGCGCTTCAGCAACT+TGG 0.469776 1.4:-73253592 MS.gene60862:CDS
AGAGAGGTAATGAATGTTGA+TGG 0.478733 1.4:-73253426 MS.gene60862:CDS
GCATTCAATCTCAGATGAAA+GGG 0.480171 1.4:-73252880 MS.gene60862:CDS
AGGTGCTGCTGTTTGTGAAT+TGG 0.480961 1.4:+73253073 None:intergenic
CTCTCTGATTTGCTTTGGTG+TGG 0.488332 1.4:+73253443 None:intergenic
GTGTTGGAATTGAAGAGAAA+TGG 0.488831 1.4:-73253838 MS.gene60862:CDS
ACAACTGAGTATAATTTGAA+TGG 0.489356 1.4:-73254252 MS.gene60862:CDS
TCAATAACCAGGGGAAGTAT+TGG 0.491273 1.4:+73252791 None:intergenic
TTGGTCTTCTAGTATGCAAC+CGG 0.498211 1.4:+73253117 None:intergenic
ACAAGTGAATTGTTAAGTAA+AGG 0.498649 1.4:-73253760 MS.gene60862:CDS
ATGTTGCCTACAAGGACAAA+AGG 0.503411 1.4:+73253053 None:intergenic
AAGGCGCTTCAGCAACTTGG+TGG 0.504237 1.4:-73253589 MS.gene60862:CDS
ACTTGAGGATGTGTGGCTAC+TGG 0.518302 1.4:+73252939 None:intergenic
TTCTTAATTGTAGCAACTTG+AGG 0.518381 1.4:+73252924 None:intergenic
AGCATTCAATCTCAGATGAA+AGG 0.522044 1.4:-73252881 MS.gene60862:CDS
AAGAGAAATGGAAGTGGTGG+TGG 0.523432 1.4:-73253826 MS.gene60862:CDS
TGTGTAGAGGATGAAGATGT+AGG 0.529070 1.4:+73252822 None:intergenic
TTAAGAAACCAGAGTTCAAG+AGG 0.534779 1.4:-73252908 MS.gene60862:CDS
TCCAACAACAAGAGTATGGT+GGG 0.538170 1.4:-73253025 MS.gene60862:CDS
AGAGAAAGCAGCGACGGTGC+TGG 0.540249 1.4:-73253639 MS.gene60862:CDS
AGAACTCACGGCAGCTACAC+CGG 0.545612 1.4:+73253723 None:intergenic
GTAACGAGTTCTAACGAAAG+AGG 0.565892 1.4:+73254436 None:intergenic
GGCAGCTACACCGGTCGAAG+AGG 0.574491 1.4:+73253732 None:intergenic
AAAATTAAAACTCAATAACC+AGG 0.575545 1.4:+73252780 None:intergenic
ATAATAAGAAGAAATCTGAC+TGG 0.576819 1.4:-73254068 MS.gene60862:CDS
AGGAAAAGAGAAAGCAGCGA+CGG 0.577313 1.4:-73253645 MS.gene60862:CDS
ACTACTACCAATACTTCCCC+TGG 0.577357 1.4:-73252798 MS.gene60862:CDS
TAAGAAAACAGTGCCTGTGT+TGG 0.581215 1.4:-73253854 MS.gene60862:CDS
TTCAGATCCTTCATCTGAAG+AGG 0.584122 1.4:-73254022 MS.gene60862:intron
AAAGGACAACCCTCTTCGAC+CGG 0.587220 1.4:-73253742 MS.gene60862:CDS
AAATTAAAACTCAATAACCA+GGG 0.590216 1.4:+73252781 None:intergenic
GCAGCTACACCGGTCGAAGA+GGG 0.597437 1.4:+73253733 None:intergenic
TTTCTCTTCAATTCCAACAC+AGG 0.600323 1.4:+73253841 None:intergenic
AACAGGGGAAATGACAAAAG+TGG 0.604173 1.4:-73252985 MS.gene60862:CDS
ATTGGTAGTAGTATGTGTAG+AGG 0.610237 1.4:+73252809 None:intergenic
AATGTCACCAGTAACAATGT+TGG 0.610482 1.4:-73253691 MS.gene60862:CDS
GTCCAACAACAAGAGTATGG+TGG 0.618792 1.4:-73253026 MS.gene60862:CDS
GAACAGACAACATCAACAGA+TGG 0.623810 1.4:-73254213 MS.gene60862:CDS
ATTAGCCGCATTAGAACTCA+CGG 0.629576 1.4:+73253711 None:intergenic
TCGTTAGAACTCGTTACACA+AGG 0.638093 1.4:-73254430 MS.gene60862:intron
GAAAGGGGTAATAACAGTGA+AGG 0.640994 1.4:-73252864 MS.gene60862:CDS
TTGAAGAGAAATGGAAGTGG+TGG 0.651388 1.4:-73253829 MS.gene60862:CDS
CATTCAATCTCAGATGAAAG+GGG 0.655574 1.4:-73252879 MS.gene60862:CDS
ACTGGTGCATAGATTCCAGA+AGG 0.656305 1.4:+73252957 None:intergenic
AGCTGCCGTGAGTTCTAATG+CGG 0.671481 1.4:-73253716 MS.gene60862:CDS
TGTAGCAACTTGAGGATGTG+TGG 0.683531 1.4:+73252932 None:intergenic
GAATTGAAGAGAAATGGAAG+TGG 0.689165 1.4:-73253832 MS.gene60862:CDS
TAGCTTCATCAACAACAACA+GGG 0.693117 1.4:-73253001 MS.gene60862:CDS
AATTAAAACTCAATAACCAG+GGG 0.700665 1.4:+73252782 None:intergenic
CCAACAACAAGAGTATGGTG+GGG 0.702776 1.4:-73253024 MS.gene60862:CDS
AGCTTCATCAACAACAACAG+GGG 0.709783 1.4:-73253000 MS.gene60862:CDS
CACACCAAAGCAAATCAGAG+AGG 0.720773 1.4:-73253442 MS.gene60862:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAATTGATTTTAATTG+AGG + chr1.4:73254153-73254172 None:intergenic 10.0%
!!! AAAATAATTTTGGATGTTTT+TGG - chr1.4:73252993-73253012 MS.gene60862:CDS 15.0%
!!! AAGTTTGTATTTTTTTTATC+TGG - chr1.4:73252953-73252972 MS.gene60862:CDS 15.0%
!!! AGTTTGTATTTTTTTTATCT+GGG - chr1.4:73252954-73252973 MS.gene60862:CDS 15.0%
!! AGTTTATTCCAATTAAGAAA+AGG - chr1.4:73253138-73253157 MS.gene60862:intron 20.0%
!! GTTTATTCCAATTAAGAAAA+GGG - chr1.4:73253139-73253158 MS.gene60862:intron 20.0%
!!! GTTTTTGTGAAAAATTGTTT+AGG - chr1.4:73253430-73253449 MS.gene60862:CDS 20.0%
!!! TTACTTTTTGTTAAACTGAA+GGG - chr1.4:73253371-73253390 MS.gene60862:intron 20.0%
!!! TTTACTTTTTGTTAAACTGA+AGG - chr1.4:73253370-73253389 MS.gene60862:intron 20.0%
!!! TTTTTCACAAAAACAAAACA+AGG + chr1.4:73253424-73253443 None:intergenic 20.0%
! AAAATTGACAACAAAAGAGT+TGG + chr1.4:73253970-73253989 None:intergenic 25.0%
! AAATTGACAACAAAAGAGTT+GGG + chr1.4:73253969-73253988 None:intergenic 25.0%
! ACAACTGAGTATAATTTGAA+TGG - chr1.4:73253062-73253081 MS.gene60862:CDS 25.0%
! ACAAGTGAATTGTTAAGTAA+AGG - chr1.4:73253554-73253573 MS.gene60862:intron 25.0%
! ATAATAAGAAGAAATCTGAC+TGG - chr1.4:73253246-73253265 MS.gene60862:intron 25.0%
! TGTTAACTCTATCTTCTTTA+TGG + chr1.4:73253529-73253548 None:intergenic 25.0%
ACTCTATCTTCTTTATGGAA+TGG + chr1.4:73253524-73253543 None:intergenic 30.0%
ATTATTACCTCTTCAGATGA+AGG + chr1.4:73253302-73253321 None:intergenic 30.0%
CATATCTACAAACCTGTAAA+TGG + chr1.4:73253938-73253957 None:intergenic 30.0%
GAAATCCTATGCAATTGAAA+GGG + chr1.4:73254074-73254093 None:intergenic 30.0%
TGAAATCCTATGCAATTGAA+AGG + chr1.4:73254075-73254094 None:intergenic 30.0%
TTCTTAATTGTAGCAACTTG+AGG + chr1.4:73254393-73254412 None:intergenic 30.0%
! CTTTCGTTATTTGTTGAACT+TGG + chr1.4:73254219-73254238 None:intergenic 30.0%
!! GGAATAAACTCTTCTAAAAC+AGG + chr1.4:73253128-73253147 None:intergenic 30.0%
!!! GTTTTTTGCTTCTGTTCTAT+AGG - chr1.4:73253846-73253865 MS.gene60862:CDS 30.0%
!!! TTTTGACTTCATCATTTGTG+AGG + chr1.4:73253914-73253933 None:intergenic 30.0%
AACGAAAGAGGAAGTTCTTT+AGG + chr1.4:73252869-73252888 None:intergenic 35.0%
AATGTCACCAGTAACAATGT+TGG - chr1.4:73253623-73253642 MS.gene60862:CDS 35.0%
AGAGAGGTAATGAATGTTGA+TGG - chr1.4:73253888-73253907 MS.gene60862:intron 35.0%
AGCATTCAATCTCAGATGAA+AGG - chr1.4:73254433-73254452 MS.gene60862:CDS 35.0%
CATTCAATCTCAGATGAAAG+GGG - chr1.4:73254435-73254454 MS.gene60862:CDS 35.0%
GAATTGAAGAGAAATGGAAG+TGG - chr1.4:73253482-73253501 MS.gene60862:intron 35.0%
GCATTCAATCTCAGATGAAA+GGG - chr1.4:73254434-73254453 MS.gene60862:CDS 35.0%
GTGTTGGAATTGAAGAGAAA+TGG - chr1.4:73253476-73253495 MS.gene60862:intron 35.0%
TAGCTTCATCAACAACAACA+GGG - chr1.4:73254313-73254332 MS.gene60862:intron 35.0%
TGAAGTCAAAAGCCATTTAC+AGG - chr1.4:73253923-73253942 MS.gene60862:intron 35.0%
TTAAGAAACCAGAGTTCAAG+AGG - chr1.4:73254406-73254425 MS.gene60862:intron 35.0%
TTAGTCCCTTTCAATTGCAT+AGG - chr1.4:73254066-73254085 MS.gene60862:CDS 35.0%
TTTCTCTTCAATTCCAACAC+AGG + chr1.4:73253476-73253495 None:intergenic 35.0%
TTTGTCCAACAACAAGAGTA+TGG - chr1.4:73254285-73254304 MS.gene60862:CDS 35.0%
! ATTGGTAGTAGTATGTGTAG+AGG + chr1.4:73254508-73254527 None:intergenic 35.0%
! GAAGAAGCCCTTTTCTTAAT+TGG + chr1.4:73253149-73253168 None:intergenic 35.0%
! TCTGTTTTGCACAGAAATAC+CGG - chr1.4:73254178-73254197 MS.gene60862:CDS 35.0%
!! ATTACCTCTCTGATTTGCTT+TGG + chr1.4:73253879-73253898 None:intergenic 35.0%
AACAGGGGAAATGACAAAAG+TGG - chr1.4:73254329-73254348 MS.gene60862:intron 40.0%
AGCTTCATCAACAACAACAG+GGG - chr1.4:73254314-73254333 MS.gene60862:intron 40.0%
ATGTTGCCTACAAGGACAAA+AGG + chr1.4:73254264-73254283 None:intergenic 40.0%
ATTAGCCGCATTAGAACTCA+CGG + chr1.4:73253606-73253625 None:intergenic 40.0%
CATCAAAAGAGACGAGAAAG+AGG - chr1.4:73253649-73253668 MS.gene60862:CDS 40.0%
GAAAGGGGTAATAACAGTGA+AGG - chr1.4:73254450-73254469 MS.gene60862:CDS 40.0%
GAACAGACAACATCAACAGA+TGG - chr1.4:73253101-73253120 MS.gene60862:CDS 40.0%
GATGTCTCCAACATTGTTAC+TGG + chr1.4:73253633-73253652 None:intergenic 40.0%
GGACAAAAATGTTGCCTACA+AGG + chr1.4:73254272-73254291 None:intergenic 40.0%
GGCTTAGATCTGTTCAGTTA+TGG - chr1.4:73253267-73253286 MS.gene60862:intron 40.0%
GTAGCTTCATCAACAACAAC+AGG - chr1.4:73254312-73254331 MS.gene60862:intron 40.0%
GTCGTTCTTGTTCTAATGCT+TGG + chr1.4:73252829-73252848 None:intergenic 40.0%
TAAGAAAACAGTGCCTGTGT+TGG - chr1.4:73253460-73253479 MS.gene60862:intron 40.0%
TCGTTAGAACTCGTTACACA+AGG - chr1.4:73252884-73252903 MS.gene60862:CDS 40.0%
TGTGTAGAGGATGAAGATGT+AGG + chr1.4:73254495-73254514 None:intergenic 40.0%
TTCAGATCCTTCATCTGAAG+AGG - chr1.4:73253292-73253311 MS.gene60862:intron 40.0%
TTGAAGAGAAATGGAAGTGG+TGG - chr1.4:73253485-73253504 MS.gene60862:intron 40.0%
! GTAACGAGTTCTAACGAAAG+AGG + chr1.4:73252881-73252900 None:intergenic 40.0%
! TCAATAACCAGGGGAAGTAT+TGG + chr1.4:73254526-73254545 None:intergenic 40.0%
! TCCAACAACAAGAGTATGGT+GGG - chr1.4:73254289-73254308 MS.gene60862:CDS 40.0%
! TTGGTCTTCTAGTATGCAAC+CGG + chr1.4:73254200-73254219 None:intergenic 40.0%
AAGAGAAATGGAAGTGGTGG+TGG - chr1.4:73253488-73253507 MS.gene60862:intron 45.0%
ACAAAAGTGGTTGCACCTTC+TGG - chr1.4:73254342-73254361 MS.gene60862:intron 45.0%
ACTACTACCAATACTTCCCC+TGG - chr1.4:73254516-73254535 MS.gene60862:CDS 45.0%
ACTGGTGCATAGATTCCAGA+AGG + chr1.4:73254360-73254379 None:intergenic 45.0%
AGGAAAAGAGAAAGCAGCGA+CGG - chr1.4:73253669-73253688 MS.gene60862:CDS 45.0%
CACACCAAAGCAAATCAGAG+AGG - chr1.4:73253872-73253891 MS.gene60862:intron 45.0%
GCTTGTCGACAACAATTGTC+TGG - chr1.4:73253035-73253054 MS.gene60862:CDS 45.0%
GTTGCACAAGCGATTCTTGA+AGG - chr1.4:73253706-73253725 MS.gene60862:CDS 45.0%
TGCTCAAACCTCTTGAACTC+TGG + chr1.4:73254417-73254436 None:intergenic 45.0%
TGTAGCAACTTGAGGATGTG+TGG + chr1.4:73254385-73254404 None:intergenic 45.0%
! CCAACAACAAGAGTATGGTG+GGG - chr1.4:73254290-73254309 MS.gene60862:CDS 45.0%
! GTCCAACAACAAGAGTATGG+TGG - chr1.4:73254288-73254307 MS.gene60862:CDS 45.0%
!! AGGTGCTGCTGTTTGTGAAT+TGG + chr1.4:73254244-73254263 None:intergenic 45.0%
!! CTCTCTGATTTGCTTTGGTG+TGG + chr1.4:73253874-73253893 None:intergenic 45.0%
!! ATATATAAAAAAAAACAAAA+AGG + chr1.4:73252921-73252940 None:intergenic 5.0%
!! TATATAAAAAAAAACAAAAA+GGG + chr1.4:73252920-73252939 None:intergenic 5.0%
!!! TTATTTTTGAAAAATAATTT+TGG - chr1.4:73252983-73253002 MS.gene60862:CDS 5.0%
AAAGGACAACCCTCTTCGAC+CGG - chr1.4:73253572-73253591 MS.gene60862:intron 50.0%
ACTTGAGGATGTGTGGCTAC+TGG + chr1.4:73254378-73254397 None:intergenic 50.0%
! AGCTGCCGTGAGTTCTAATG+CGG - chr1.4:73253598-73253617 MS.gene60862:CDS 50.0%
! CCCCACCATACTCTTGTTGT+TGG + chr1.4:73254293-73254312 None:intergenic 50.0%
! GCAGCACCTTTTGTCCTTGT+AGG - chr1.4:73254255-73254274 MS.gene60862:CDS 50.0%
!! TTGAAGGCGCTTCAGCAACT+TGG - chr1.4:73253722-73253741 MS.gene60862:CDS 50.0%
AGAACTCACGGCAGCTACAC+CGG + chr1.4:73253594-73253613 None:intergenic 55.0%
! AAGGCGCTTCAGCAACTTGG+TGG - chr1.4:73253725-73253744 MS.gene60862:CDS 55.0%
AGAGAAAGCAGCGACGGTGC+TGG - chr1.4:73253675-73253694 MS.gene60862:CDS 60.0%
GCAGCTACACCGGTCGAAGA+GGG + chr1.4:73253584-73253603 None:intergenic 60.0%
GGCAGCTACACCGGTCGAAG+AGG + chr1.4:73253585-73253604 None:intergenic 65.0%
!! GCAGCGACGGTGCTGGTCGC+AGG - chr1.4:73253682-73253701 MS.gene60862:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 73252791 73254545 73252791 ID=MS.gene60862
chr1.4 mRNA 73252791 73254545 73252791 ID=MS.gene60862.t1;Parent=MS.gene60862
chr1.4 exon 73254431 73254545 73254431 ID=MS.gene60862.t1.exon1;Parent=MS.gene60862.t1
chr1.4 CDS 73254431 73254545 73254431 ID=cds.MS.gene60862.t1;Parent=MS.gene60862.t1
chr1.4 exon 73254023 73254309 73254023 ID=MS.gene60862.t1.exon2;Parent=MS.gene60862.t1
chr1.4 CDS 73254023 73254309 73254023 ID=cds.MS.gene60862.t1;Parent=MS.gene60862.t1
chr1.4 exon 73253581 73253884 73253581 ID=MS.gene60862.t1.exon3;Parent=MS.gene60862.t1
chr1.4 CDS 73253581 73253884 73253581 ID=cds.MS.gene60862.t1;Parent=MS.gene60862.t1
chr1.4 exon 73253392 73253468 73253392 ID=MS.gene60862.t1.exon4;Parent=MS.gene60862.t1
chr1.4 CDS 73253392 73253468 73253392 ID=cds.MS.gene60862.t1;Parent=MS.gene60862.t1
chr1.4 exon 73252791 73253144 73252791 ID=MS.gene60862.t1.exon5;Parent=MS.gene60862.t1
chr1.4 CDS 73252791 73253144 73252791 ID=cds.MS.gene60862.t1;Parent=MS.gene60862.t1
Gene Sequence

>MS.gene60862

ATGCAGCGTTTCAAAATGGGTTTCTCTGATTATATCCAAGCATTAGAACAAGAACGACGTAAGATTCAAGTCTTTCCTAAAGAACTTCCTCTTTCGTTAGAACTCGTTACACAAGGTACGTACATACCCTTTTTGTTTTTTTTTATATATGATTATGAGTTAAAGTTTGTATTTTTTTTATCTGGGTTTGTTTTATTTTTGAAAAATAATTTTGGATGTTTTTGGTTGATTATTAGCTATTGAAGCTTGTCGACAACAATTGTCTGGAACAACAACTGAGTATAATTTGAATGGACAATCTGAGTGTTCTGAACAGACAACATCAACAGATGGACCTGTTTTAGAAGAGTTTATTCCAATTAAGAAAAGGGCTTCTTCTTATTCTCAAGAAGTGTTTGATGATGTAGAAGATTATGATCAACATTTTCATCATAAGCAACAAAAACTTTCATTAGATAATAAGAAGAAATCTGACTGGCTTAGATCTGTTCAGTTATGGAATTCAGATCCTTCATCTGAAGAGGTAATAATAAAATCATAGCTTATTTTTCAATTATTGTTGATTGTTGTTTTAGTTTTTTTACTTTTTGTTAAACTGAAGGGAACAAACAATGAATTATAAGTAATTGACCTTGTTTTGTTTTTGTGAAAAATTGTTTAGGATGTTACTAAGAAAACAGTGCCTGTGTTGGAATTGAAGAGAAATGGAAGTGGTGGTGGTGCTTTTCATCCATTCCATAAAGAAGATAGAGTTAACAACAAGACAAGTGAATTGTTAAGTAAAGGACAACCCTCTTCGACCGGTGTAGCTGCCGTGAGTTCTAATGCGGCTAATGTCACCAGTAACAATGTTGGAGACATCAAAAGAGACGAGAAAGAGGAAAAGAGAAAGCAGCGACGGTGCTGGTCGCAGGAGTTGCACAAGCGATTCTTGAAGGCGCTTCAGCAACTTGGTGGTGCAGATTGTGAGTATCTTAATATATACATCAAGTTCTGATTGTTATGTATTCATGTTGTTAATGTAATTAACTGCAAATAGAATAAATGTTTAATGTGTTTTTTGCTTCTGTTCTATAGGTGCCACACCAAAGCAAATCAGAGAGGTAATGAATGTTGATGGCCTCACAAATGATGAAGTCAAAAGCCATTTACAGGTTTGTAGATATGTTCTTCTTCCCAACTCTTTTGTTGTCAATTTTCATCTGAGTATTTGTTTTGATTTTGTGATTTCTATTATCTAGTTGAATCTTTTAGTTTTAACTGTTTCATCTTGTTTTAGTCCCTTTCAATTGCATAGGATTTCAAAACAAACGAGTGATAGTTTTTGTTGTTACAGAAGTTACAACAACAGTTTAATATCCTCAATTAAAATCAATTTTTTTTTCTTTCTGTTTTGCACAGAAATACCGGTTGCATACTAGAAGACCAAGTTCAACAAATAACGAAAGTGCCAATTCACAAACAGCAGCACCTTTTGTCCTTGTAGGCAACATTTTTGTCCAACAACAAGAGTATGGTGGGGTAGCTTCATCAACAACAACAGGGGAAATGACAAAAGTGGTTGCACCTTCTGGAATCTATGCACCAGTAGCCACACATCCTCAAGTTGCTACAATTAAGAAACCAGAGTTCAAGAGGTTTGAGCATTCAATCTCAGATGAAAGGGGTAATAACAGTGAAGGTGCTGTTCATTCAAATTCACCTACATCTTCATCCTCTACACATACTACTACCAATACTTCCCCTGGTTATTGA
Protein sequence

>MS.gene60862.t1

MQRFKMGFSDYIQALEQERRKIQVFPKELPLSLELVTQAIEACRQQLSGTTTEYNLNGQSECSEQTTSTDGPVLEEFIPIKKRASSYSQEVFDDVEDYDQHFHHKQQKLSLDNKKKSDWLRSVQLWNSDPSSEEDVTKKTVPVLELKRNGSGGGAFHPFHKEDRVNNKTSELLSKGQPSSTGVAAVSSNAANVTSNNVGDIKRDEKEEKRKQRRCWSQELHKRFLKALQQLGGADCATPKQIREVMNVDGLTNDEVKSHLQKYRLHTRRPSSTNNESANSQTAAPFVLVGNIFVQQQEYGGVASSTTTGEMTKVVAPSGIYAPVATHPQVATIKKPEFKRFEHSISDERGNNSEGAVHSNSPTSSSSTHTTTNTSPGY