AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60863

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60863.t1 MTR_1g093095 98.299 294 5 0 1 294 1 294 0.0 608
MS.gene60863.t1 MTR_1g112270 35.862 145 78 5 107 249 65 196 1.90e-20 91.7
MS.gene60863.t1 MTR_1g112270 35.862 145 78 5 107 249 76 207 2.24e-20 91.3
MS.gene60863.t1 MTR_4g059870 34.507 142 81 4 109 249 61 191 2.26e-20 91.3
MS.gene60863.t1 MTR_1g094115 36.429 140 74 5 112 249 56 182 3.47e-20 90.9
MS.gene60863.t1 MTR_1g094115 35.172 145 79 5 107 249 68 199 4.51e-20 90.5
MS.gene60863.t1 MTR_8g017210 33.333 156 89 6 109 262 85 227 7.18e-20 89.7
MS.gene60863.t1 MTR_1g016010 34.161 161 91 6 91 249 47 194 7.76e-20 89.4
MS.gene60863.t1 MTR_2g093960 35.211 142 80 4 109 249 55 185 1.40e-19 88.6
MS.gene60863.t1 MTR_2g099990 35.172 145 79 6 107 249 67 198 5.04e-19 87.0
MS.gene60863.t1 MTR_2g099990 35.211 142 77 6 110 249 1 129 3.48e-18 84.3
MS.gene60863.t1 MTR_2g090745 33.553 152 88 5 107 257 28 167 8.20e-18 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60863.t1 AT1G13290 65.854 246 60 5 23 258 12 243 1.51e-108 317
MS.gene60863.t1 AT3G57670 71.429 196 40 4 99 292 201 382 5.94e-98 293
MS.gene60863.t1 AT1G51220 55.000 260 100 5 6 264 81 324 6.83e-95 284
MS.gene60863.t1 AT3G20880 77.987 159 32 1 97 255 238 393 7.07e-94 284
MS.gene60863.t1 AT1G08290 73.810 168 38 3 99 265 164 326 5.02e-93 279
MS.gene60863.t1 AT1G34790 75.159 157 36 1 100 256 130 283 3.36e-88 265
MS.gene60863.t1 AT1G34370 41.176 153 84 5 107 255 27 177 3.33e-26 105
MS.gene60863.t1 AT1G34370 41.176 153 84 5 107 255 237 387 1.25e-25 106
MS.gene60863.t1 AT1G34370 41.176 153 84 5 107 255 237 387 1.25e-25 106
MS.gene60863.t1 AT5G22890 40.645 155 84 7 106 255 209 360 1.73e-24 101
MS.gene60863.t1 AT5G03150 35.211 142 80 4 109 249 76 206 6.15e-21 92.8
MS.gene60863.t1 AT1G55110 33.758 157 90 5 107 261 84 228 8.01e-21 92.4
MS.gene60863.t1 AT1G55110 33.758 157 90 5 107 261 84 228 8.01e-21 92.4
MS.gene60863.t1 AT1G55110 33.758 157 90 5 107 261 84 228 8.01e-21 92.4
MS.gene60863.t1 AT5G44160 32.178 202 106 8 63 257 20 197 2.44e-20 90.9
MS.gene60863.t1 AT2G02070 35.443 158 87 5 94 249 60 204 3.47e-20 90.9
MS.gene60863.t1 AT2G02070 35.443 158 87 5 94 249 60 204 3.47e-20 90.9
MS.gene60863.t1 AT2G02080 36.552 145 77 5 107 249 75 206 4.06e-20 90.5
MS.gene60863.t1 AT2G02080 36.552 145 77 5 107 249 75 206 4.06e-20 90.5
MS.gene60863.t1 AT2G02080 36.552 145 77 5 107 249 75 206 4.06e-20 90.5
MS.gene60863.t1 AT2G02080 36.552 145 77 5 107 249 75 206 4.06e-20 90.5
MS.gene60863.t1 AT3G45260 34.615 156 90 4 109 263 62 206 4.32e-20 90.1
MS.gene60863.t1 AT3G45260 34.615 156 90 4 109 263 62 206 4.32e-20 90.1
MS.gene60863.t1 AT5G60470 35.211 142 80 4 109 249 58 188 6.78e-20 89.7
MS.gene60863.t1 AT5G60470 35.211 142 80 4 109 249 63 193 7.85e-20 89.4
MS.gene60863.t1 AT5G60470 35.211 142 80 4 109 249 67 197 8.05e-20 89.4
MS.gene60863.t1 AT5G66730 34.722 144 81 4 107 249 53 184 1.10e-19 89.4
MS.gene60863.t1 AT1G14580 37.241 145 76 6 107 249 74 205 1.31e-19 89.0
MS.gene60863.t1 AT1G14580 37.241 145 76 6 107 249 74 205 1.31e-19 89.0
MS.gene60863.t1 AT1G14580 37.241 145 76 6 107 249 88 219 1.56e-19 88.6
MS.gene60863.t1 AT1G03840 31.638 177 107 6 83 257 35 199 2.79e-19 88.2
MS.gene60863.t1 AT2G02080 36.620 142 75 5 110 249 1 129 3.38e-19 87.4
MS.gene60863.t1 AT2G02080 36.620 142 75 5 110 249 1 129 3.38e-19 87.4
MS.gene60863.t1 AT3G50700 35.172 145 79 5 107 249 55 186 5.48e-19 87.0
MS.gene60863.t1 AT5G44160 34.667 150 85 5 109 257 21 158 1.19e-18 85.9
MS.gene60863.t1 AT1G03840 31.844 179 106 6 83 257 35 201 1.59e-18 85.9
MS.gene60863.t1 AT3G13810 32.051 156 94 4 107 261 86 230 2.39e-18 85.5
MS.gene60863.t1 AT3G13810 32.895 152 90 4 107 257 92 232 2.56e-18 85.1
MS.gene60863.t1 AT3G13810 32.051 156 94 4 107 261 91 235 2.63e-18 85.1
MS.gene60863.t1 AT3G13810 32.051 156 94 4 107 261 91 235 2.63e-18 85.1
MS.gene60863.t1 AT3G13810 32.051 156 94 4 107 261 72 216 2.75e-18 85.1
MS.gene60863.t1 AT3G13810 32.895 152 90 4 107 257 78 218 2.87e-18 85.1
MS.gene60863.t1 AT4G02670 34.028 144 83 4 107 249 76 208 3.79e-18 84.3
MS.gene60863.t1 AT4G02670 34.028 144 83 4 107 249 74 206 3.89e-18 84.3

Find 84 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCGAGTTTGGGAGGTATT+TGG 0.247423 1.4:-73271270 None:intergenic
CTCCCATTATTCCTCAATAT+TGG 0.256317 1.4:+73271443 MS.gene60863:CDS
AGTGAAAGTTGGATTAATTA+AGG 0.285981 1.4:-73271404 None:intergenic
GATCCAATATTGAGGAATAA+TGG 0.295991 1.4:-73271446 None:intergenic
AAAGGACCGGAGTCTCTAAA+AGG 0.306782 1.4:+73271841 MS.gene60863:CDS
GATGAAGATGATGCTGGTTT+TGG 0.319383 1.4:-73271865 None:intergenic
TGCAGACAGTGCAAGAAAAC+TGG 0.325433 1.4:-73271501 None:intergenic
TCTCCTGCTCAAATCCTTGT+TGG 0.331954 1.4:+73271472 MS.gene60863:CDS
TTCCTCTCTCTTGCTCCTCC+TGG 0.347551 1.4:+73271244 MS.gene60863:CDS
AAAGATCACGTTCGTGCGTT+TGG 0.349116 1.4:+73272141 MS.gene60863:CDS
AGAAGAATTGTGGGAAGCTT+TGG 0.351421 1.4:+73272076 MS.gene60863:CDS
AATGGGAGTGTGAGATGGTT+TGG 0.355414 1.4:-73271428 None:intergenic
TTCTCTTGTAGTGTGTTTGA+AGG 0.366292 1.4:-73271978 None:intergenic
AGGGATAGTAGTAGTAATAG+TGG 0.376112 1.4:-73271302 None:intergenic
AGAGAGGTAGTAGTAGAAAT+AGG 0.389319 1.4:-73271340 None:intergenic
ATCCAATATTGAGGAATAAT+GGG 0.396877 1.4:-73271445 None:intergenic
CACGTTCGTGCGTTTGGTAA+TGG 0.397696 1.4:+73272147 MS.gene60863:CDS
CTACATTTACAGCACAAGTA+TGG 0.418258 1.4:-73271372 None:intergenic
GATGGTTTGGAAGTGAAAGT+TGG 0.427167 1.4:-73271415 None:intergenic
TCTTTCAGTGGTCTTGCCCT+TGG 0.430630 1.4:-73271946 None:intergenic
CATTTGCTGTGAGAGGAGAT+TGG 0.436076 1.4:+73272043 MS.gene60863:CDS
TAGTAGTAATAGTGGGATGA+GGG 0.441652 1.4:-73271294 None:intergenic
TTGGGAGGTATTTGGTGGCC+AGG 0.460715 1.4:-73271262 None:intergenic
GGGGACATGGATCTCAATAT+AGG 0.462797 1.4:+73271818 MS.gene60863:CDS
TCTCTTGTAGTGTGTTTGAA+GGG 0.467920 1.4:-73271977 None:intergenic
GTAGTAGTAATAGTGGGATG+AGG 0.468089 1.4:-73271295 None:intergenic
GCTTCCCACAATTCTTCTCA+TGG 0.476053 1.4:-73272071 None:intergenic
GAAATAGGTATTTGATGCAA+GGG 0.489738 1.4:-73271325 None:intergenic
CATGTTGTTGAAACGGTTGA+AGG 0.493614 1.4:-73271530 None:intergenic
AGGAGCAAGAGAGAGGAAGA+TGG 0.499455 1.4:-73271239 None:intergenic
GGGATAGTAGTAGTAATAGT+GGG 0.503268 1.4:-73271301 None:intergenic
CGAGTTTGGGAGGTATTTGG+TGG 0.508108 1.4:-73271267 None:intergenic
CCATGTTACTGTTGTGCTGA+GGG 0.513723 1.4:+73271901 MS.gene60863:CDS
AGAAATAGGTATTTGATGCA+AGG 0.514616 1.4:-73271326 None:intergenic
GAGGGAAATCATTCGAGTGC+AGG 0.521978 1.4:+73272007 MS.gene60863:CDS
ATCTAAGCTGCTGCTGCAAG+AGG 0.522289 1.4:-73271206 None:intergenic
AACTCAAAGATTCCAATGTG+TGG 0.530905 1.4:-73272179 None:intergenic
ACAAGGATTTGAGCAGGAGA+AGG 0.531708 1.4:-73271469 None:intergenic
GGACCCATGAGAAGAATTGT+GGG 0.531894 1.4:+73272067 MS.gene60863:CDS
ATTCCAATGTGTGGGGAGCA+TGG 0.532297 1.4:-73272170 None:intergenic
CAACCGTTTCAACAACATGC+AGG 0.536317 1.4:+73271534 MS.gene60863:CDS
CATTCGAGTGCAGGAAGTGT+AGG 0.544080 1.4:+73272016 MS.gene60863:CDS
ACTCAAAGATTCCAATGTGT+GGG 0.544189 1.4:-73272178 None:intergenic
GAAACAACATAAACTATCCA+AGG 0.546681 1.4:+73271929 MS.gene60863:CDS
GAACCTGCATGTTGTTGAAA+CGG 0.548476 1.4:-73271537 None:intergenic
CTTCCCACAATTCTTCTCAT+GGG 0.550992 1.4:-73272070 None:intergenic
AGGACCCATGAGAAGAATTG+TGG 0.552247 1.4:+73272066 MS.gene60863:CDS
AGCATTGATGAAGATGATGC+TGG 0.557005 1.4:-73271871 None:intergenic
AAGGACCGGAGTCTCTAAAA+GGG 0.557565 1.4:+73271842 MS.gene60863:CDS
TTGTATGGTAGATGCATATG+TGG 0.564484 1.4:+73271797 MS.gene60863:intron
GGAGGTATTTGGTGGCCAGG+AGG 0.567766 1.4:-73271259 None:intergenic
TACATGGAAGAAGAAGAAAG+TGG 0.570369 1.4:+73272228 MS.gene60863:CDS
ATAGGTATTTGATGCAAGGG+AGG 0.571468 1.4:-73271322 None:intergenic
GGACCAACAAGGATTTGAGC+AGG 0.574385 1.4:-73271475 None:intergenic
CAAGGATTTGAGCAGGAGAA+GGG 0.578543 1.4:-73271468 None:intergenic
TGGCCATGCTCCCCACACAT+TGG 0.581176 1.4:+73272167 MS.gene60863:CDS
TAAAGTAAATGAAAATTACA+TGG 0.583660 1.4:+73272212 MS.gene60863:CDS
AGTAGTAATAGTGGGATGAG+GGG 0.585249 1.4:-73271293 None:intergenic
GCAGACAGTGCAAGAAAACT+GGG 0.587578 1.4:-73271500 None:intergenic
GGCCAGGAGGAGCAAGAGAG+AGG 0.593784 1.4:-73271246 None:intergenic
GGAATAATGGGAGTGTGAGA+TGG 0.594723 1.4:-73271433 None:intergenic
TTGCTGTGAGAGGAGATTGG+AGG 0.596970 1.4:+73272046 MS.gene60863:CDS
ATCTCAATATAGGAAAGGAC+CGG 0.597133 1.4:+73271828 MS.gene60863:CDS
AGGGTTCTGAAATCTTTCAG+TGG 0.606548 1.4:-73271958 None:intergenic
GGAGAAGGGATCCAATATTG+AGG 0.614687 1.4:-73271454 None:intergenic
ACCATGTTACTGTTGTGCTG+AGG 0.615026 1.4:+73271900 MS.gene60863:CDS
AGGAAGACATTTGCTGTGAG+AGG 0.616285 1.4:+73272036 MS.gene60863:CDS
CATGGATCTCAATATAGGAA+AGG 0.616598 1.4:+73271823 MS.gene60863:CDS
TGTATGGTAGATGCATATGT+GGG 0.620648 1.4:+73271798 MS.gene60863:intron
ACACACTACAAGAGAAAGCA+CGG 0.625692 1.4:+73271985 MS.gene60863:CDS
CCCTCAGCACAACAGTAACA+TGG 0.627644 1.4:-73271901 None:intergenic
GTAGTAATAGTGGGATGAGG+GGG 0.632310 1.4:-73271292 None:intergenic
TAGATGCATATGTGGGGACA+TGG 0.632523 1.4:+73271805 MS.gene60863:intron
ACTACAAGAGAAAGCACGGA+GGG 0.635203 1.4:+73271989 MS.gene60863:CDS
AGTATGGTCATGGTGAAGAG+AGG 0.640967 1.4:-73271356 None:intergenic
CTCAAAGATTCCAATGTGTG+GGG 0.641103 1.4:-73272177 None:intergenic
TAGGTATTTGATGCAAGGGA+GGG 0.641902 1.4:-73271321 None:intergenic
AAACAACATAAACTATCCAA+GGG 0.649476 1.4:+73271930 MS.gene60863:CDS
TTACAGCACAAGTATGGTCA+TGG 0.652632 1.4:-73271366 None:intergenic
ACAGTGCAAGAAAACTGGGT+AGG 0.667615 1.4:-73271496 None:intergenic
AAAACTGGGTAGGACCAACA+AGG 0.677453 1.4:-73271486 None:intergenic
GTACTGATGATGAGTTTGCA+TGG 0.690956 1.4:-73271161 None:intergenic
CACTACAAGAGAAAGCACGG+AGG 0.743501 1.4:+73271988 MS.gene60863:CDS
GTATGGTAGATGCATATGTG+GGG 0.763722 1.4:+73271799 MS.gene60863:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGGATAAAAATATAATAA+TGG - chr1.4:73271565-73271584 None:intergenic 10.0%
!! AAACATGATTCATATATATA+TGG + chr1.4:73271752-73271771 MS.gene60863:intron 15.0%
!! CTAATGAATGATTAATTTAA+AGG - chr1.4:73271654-73271673 None:intergenic 15.0%
!! TAAAGTAAATGAAAATTACA+TGG + chr1.4:73272212-73272231 MS.gene60863:CDS 15.0%
!! TCATGATATAGTAAAAATAA+AGG - chr1.4:73271583-73271602 None:intergenic 15.0%
!! TATGATATTGTTGTATTGTA+TGG + chr1.4:73271782-73271801 MS.gene60863:intron 20.0%
! AAACAACATAAACTATCCAA+GGG + chr1.4:73271930-73271949 MS.gene60863:CDS 25.0%
! AGTGAAAGTTGGATTAATTA+AGG - chr1.4:73271407-73271426 None:intergenic 25.0%
! ATCCAATATTGAGGAATAAT+GGG - chr1.4:73271448-73271467 None:intergenic 25.0%
! GAATGATTAATTTAAAGGCA+AGG - chr1.4:73271649-73271668 None:intergenic 25.0%
AGAAATAGGTATTTGATGCA+AGG - chr1.4:73271329-73271348 None:intergenic 30.0%
AGCTCAATATGATTCATTCA+TGG - chr1.4:73271716-73271735 None:intergenic 30.0%
GAAACAACATAAACTATCCA+AGG + chr1.4:73271929-73271948 MS.gene60863:CDS 30.0%
GAAATAGGTATTTGATGCAA+GGG - chr1.4:73271328-73271347 None:intergenic 30.0%
GATCCAATATTGAGGAATAA+TGG - chr1.4:73271449-73271468 None:intergenic 30.0%
TCATTCATGGAAGAAAAGTA+AGG - chr1.4:73271703-73271722 None:intergenic 30.0%
!! TTGGAATCTTTGAGTTTTGA+TGG + chr1.4:73272186-73272205 MS.gene60863:CDS 30.0%
AACTCAAAGATTCCAATGTG+TGG - chr1.4:73272182-73272201 None:intergenic 35.0%
ACTCAAAGATTCCAATGTGT+GGG - chr1.4:73272181-73272200 None:intergenic 35.0%
AGAGAGGTAGTAGTAGAAAT+AGG - chr1.4:73271343-73271362 None:intergenic 35.0%
AGGGATAGTAGTAGTAATAG+TGG - chr1.4:73271305-73271324 None:intergenic 35.0%
ATCTCAATATAGGAAAGGAC+CGG + chr1.4:73271828-73271847 MS.gene60863:CDS 35.0%
CATGGATCTCAATATAGGAA+AGG + chr1.4:73271823-73271842 MS.gene60863:CDS 35.0%
CTACATTTACAGCACAAGTA+TGG - chr1.4:73271375-73271394 None:intergenic 35.0%
CTCCCATTATTCCTCAATAT+TGG + chr1.4:73271443-73271462 MS.gene60863:CDS 35.0%
GGGATAGTAGTAGTAATAGT+GGG - chr1.4:73271304-73271323 None:intergenic 35.0%
TACATGGAAGAAGAAGAAAG+TGG + chr1.4:73272228-73272247 MS.gene60863:CDS 35.0%
TCTCTTGTAGTGTGTTTGAA+GGG - chr1.4:73271980-73271999 None:intergenic 35.0%
TGTATGGTAGATGCATATGT+GGG + chr1.4:73271798-73271817 MS.gene60863:intron 35.0%
TTCTCTTGTAGTGTGTTTGA+AGG - chr1.4:73271981-73272000 None:intergenic 35.0%
TTGTATGGTAGATGCATATG+TGG + chr1.4:73271797-73271816 MS.gene60863:intron 35.0%
! TAGTAGTAATAGTGGGATGA+GGG - chr1.4:73271297-73271316 None:intergenic 35.0%
!!! AAGCTTTGGTTTTGTATCTG+TGG + chr1.4:73272090-73272109 MS.gene60863:CDS 35.0%
ACACACTACAAGAGAAAGCA+CGG + chr1.4:73271985-73272004 MS.gene60863:CDS 40.0%
AGAAGAATTGTGGGAAGCTT+TGG + chr1.4:73272076-73272095 MS.gene60863:CDS 40.0%
ATAGGTATTTGATGCAAGGG+AGG - chr1.4:73271325-73271344 None:intergenic 40.0%
CATGTTGTTGAAACGGTTGA+AGG - chr1.4:73271533-73271552 None:intergenic 40.0%
CTCAAAGATTCCAATGTGTG+GGG - chr1.4:73272180-73272199 None:intergenic 40.0%
CTTCCCACAATTCTTCTCAT+GGG - chr1.4:73272073-73272092 None:intergenic 40.0%
GAACCTGCATGTTGTTGAAA+CGG - chr1.4:73271540-73271559 None:intergenic 40.0%
GTATGGTAGATGCATATGTG+GGG + chr1.4:73271799-73271818 MS.gene60863:intron 40.0%
TAGGTATTTGATGCAAGGGA+GGG - chr1.4:73271324-73271343 None:intergenic 40.0%
TTACAGCACAAGTATGGTCA+TGG - chr1.4:73271369-73271388 None:intergenic 40.0%
! AGTAGTAATAGTGGGATGAG+GGG - chr1.4:73271296-73271315 None:intergenic 40.0%
! GATGGTTTGGAAGTGAAAGT+TGG - chr1.4:73271418-73271437 None:intergenic 40.0%
! GTAGTAGTAATAGTGGGATG+AGG - chr1.4:73271298-73271317 None:intergenic 40.0%
!! AGCATTGATGAAGATGATGC+TGG - chr1.4:73271874-73271893 None:intergenic 40.0%
!! AGGGTTCTGAAATCTTTCAG+TGG - chr1.4:73271961-73271980 None:intergenic 40.0%
!!! GATGAAGATGATGCTGGTTT+TGG - chr1.4:73271868-73271887 None:intergenic 40.0%
AAAACTGGGTAGGACCAACA+AGG - chr1.4:73271489-73271508 None:intergenic 45.0%
AAAGATCACGTTCGTGCGTT+TGG + chr1.4:73272141-73272160 MS.gene60863:CDS 45.0%
AAAGGACCGGAGTCTCTAAA+AGG + chr1.4:73271841-73271860 MS.gene60863:CDS 45.0%
ACAAGGATTTGAGCAGGAGA+AGG - chr1.4:73271472-73271491 None:intergenic 45.0%
ACAGTGCAAGAAAACTGGGT+AGG - chr1.4:73271499-73271518 None:intergenic 45.0%
ACCATGTTACTGTTGTGCTG+AGG + chr1.4:73271900-73271919 MS.gene60863:CDS 45.0%
AGGACCCATGAGAAGAATTG+TGG + chr1.4:73272066-73272085 MS.gene60863:CDS 45.0%
AGTATGGTCATGGTGAAGAG+AGG - chr1.4:73271359-73271378 None:intergenic 45.0%
CAACCGTTTCAACAACATGC+AGG + chr1.4:73271534-73271553 MS.gene60863:CDS 45.0%
CAAGGATTTGAGCAGGAGAA+GGG - chr1.4:73271471-73271490 None:intergenic 45.0%
CCATGTTACTGTTGTGCTGA+GGG + chr1.4:73271901-73271920 MS.gene60863:CDS 45.0%
GCAGACAGTGCAAGAAAACT+GGG - chr1.4:73271503-73271522 None:intergenic 45.0%
GCTTCCCACAATTCTTCTCA+TGG - chr1.4:73272074-73272093 None:intergenic 45.0%
GGACCCATGAGAAGAATTGT+GGG + chr1.4:73272067-73272086 MS.gene60863:CDS 45.0%
GGAGAAGGGATCCAATATTG+AGG - chr1.4:73271457-73271476 None:intergenic 45.0%
GGGGACATGGATCTCAATAT+AGG + chr1.4:73271818-73271837 MS.gene60863:CDS 45.0%
TAGATGCATATGTGGGGACA+TGG + chr1.4:73271805-73271824 MS.gene60863:intron 45.0%
TCTCCTGCTCAAATCCTTGT+TGG + chr1.4:73271472-73271491 MS.gene60863:CDS 45.0%
TGCAGACAGTGCAAGAAAAC+TGG - chr1.4:73271504-73271523 None:intergenic 45.0%
! AAGGACCGGAGTCTCTAAAA+GGG + chr1.4:73271842-73271861 MS.gene60863:CDS 45.0%
! AATGGGAGTGTGAGATGGTT+TGG - chr1.4:73271431-73271450 None:intergenic 45.0%
! ACTACAAGAGAAAGCACGGA+GGG + chr1.4:73271989-73272008 MS.gene60863:CDS 45.0%
! GGAATAATGGGAGTGTGAGA+TGG - chr1.4:73271436-73271455 None:intergenic 45.0%
! GTAGTAATAGTGGGATGAGG+GGG - chr1.4:73271295-73271314 None:intergenic 45.0%
!! AGGAAGACATTTGCTGTGAG+AGG + chr1.4:73272036-73272055 MS.gene60863:CDS 45.0%
!! CATTTGCTGTGAGAGGAGAT+TGG + chr1.4:73272043-73272062 MS.gene60863:CDS 45.0%
!! TTTGGCCCTTTTAGAGACTC+CGG - chr1.4:73271850-73271869 None:intergenic 45.0%
AGGAGCAAGAGAGAGGAAGA+TGG - chr1.4:73271242-73271261 None:intergenic 50.0%
ATCTAAGCTGCTGCTGCAAG+AGG - chr1.4:73271209-73271228 None:intergenic 50.0%
ATTCCAATGTGTGGGGAGCA+TGG - chr1.4:73272173-73272192 None:intergenic 50.0%
CACGTTCGTGCGTTTGGTAA+TGG + chr1.4:73272147-73272166 MS.gene60863:CDS 50.0%
CCCTCAGCACAACAGTAACA+TGG - chr1.4:73271904-73271923 None:intergenic 50.0%
GAGGGAAATCATTCGAGTGC+AGG + chr1.4:73272007-73272026 MS.gene60863:CDS 50.0%
GGACCAACAAGGATTTGAGC+AGG - chr1.4:73271478-73271497 None:intergenic 50.0%
TTGCTGTGAGAGGAGATTGG+AGG + chr1.4:73272046-73272065 MS.gene60863:CDS 50.0%
! CACTACAAGAGAAAGCACGG+AGG + chr1.4:73271988-73272007 MS.gene60863:CDS 50.0%
! CATTCGAGTGCAGGAAGTGT+AGG + chr1.4:73272016-73272035 MS.gene60863:CDS 50.0%
! TCTTTCAGTGGTCTTGCCCT+TGG - chr1.4:73271949-73271968 None:intergenic 50.0%
!! CGAGTTTGGGAGGTATTTGG+TGG - chr1.4:73271270-73271289 None:intergenic 50.0%
!! GTGCGAGTTTGGGAGGTATT+TGG - chr1.4:73271273-73271292 None:intergenic 50.0%
TTCCTCTCTCTTGCTCCTCC+TGG + chr1.4:73271244-73271263 MS.gene60863:CDS 55.0%
!! TTGGGAGGTATTTGGTGGCC+AGG - chr1.4:73271265-73271284 None:intergenic 55.0%
TGGCCATGCTCCCCACACAT+TGG + chr1.4:73272167-73272186 MS.gene60863:CDS 60.0%
! GGATGAGGGGGTGCGAGTTT+GGG - chr1.4:73271283-73271302 None:intergenic 60.0%
!! GGAGGTATTTGGTGGCCAGG+AGG - chr1.4:73271262-73271281 None:intergenic 60.0%
GGCCAGGAGGAGCAAGAGAG+AGG - chr1.4:73271249-73271268 None:intergenic 65.0%
! GGGATGAGGGGGTGCGAGTT+TGG - chr1.4:73271284-73271303 None:intergenic 65.0%
! TGAGGGGGTGCGAGTTTGGG+AGG - chr1.4:73271280-73271299 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 73271163 73272299 73271163 ID=MS.gene60863
chr1.4 mRNA 73271163 73272299 73271163 ID=MS.gene60863.t1;Parent=MS.gene60863
chr1.4 exon 73271163 73271555 73271163 ID=MS.gene60863.t1.exon1;Parent=MS.gene60863.t1
chr1.4 CDS 73271163 73271555 73271163 ID=cds.MS.gene60863.t1;Parent=MS.gene60863.t1
chr1.4 exon 73271808 73272299 73271808 ID=MS.gene60863.t1.exon2;Parent=MS.gene60863.t1
chr1.4 CDS 73271808 73272299 73271808 ID=cds.MS.gene60863.t1;Parent=MS.gene60863.t1
Gene Sequence

>MS.gene60863

ATGCAAACTCATCATCAGTACTACTCATTCTCTTGTGAGAGTGCCTCTTGCAGCAGCAGCTTAGATGATGAAGATTCCATCTTCCTCTCTCTTGCTCCTCCTGGCCACCAAATACCTCCCAAACTCGCACCCCCTCATCCCACTATTACTACTACTATCCCTCCCTTGCATCAAATACCTATTTCTACTACTACCTCTCTTCACCATGACCATACTTGTGCTGTAAATGTAGCACTTAATGCCTTAATTAATCCAACTTTCACTTCCAAACCATCTCACACTCCCATTATTCCTCAATATTGGATCCCTTCTCCTGCTCAAATCCTTGTTGGTCCTACCCAGTTTTCTTGCACTGTCTGCAACAAAACCTTCAACCGTTTCAACAACATGCAGGTTCATCCATTATTATATTTTTATCCTTTATTTTTACTATATCATGACTAAATATCTGATACAACATTTTTCTCTCATATGAAACATAAACCTTGCCTTTAAATTAATCATTCATTAGCACTAATATAGTGTCATTTTTGTGCACCTTACTTTTCTTCCATGAATGAATCATATTGAGCTTACTATATTAATATCAAAACATGATTCATATATATATGGTACTAGTTATGATATTGTTGTATTGTATGGTAGATGCATATGTGGGGACATGGATCTCAATATAGGAAAGGACCGGAGTCTCTAAAAGGGCCAAAACCAGCATCATCTTCATCAATGCTGAAACTACCATGTTACTGTTGTGCTGAGGGTTGCAGAAACAACATAAACTATCCAAGGGCAAGACCACTGAAAGATTTCAGAACCCTTCAAACACACTACAAGAGAAAGCACGGAGGGAAATCATTCGAGTGCAGGAAGTGTAGGAAGACATTTGCTGTGAGAGGAGATTGGAGGACCCATGAGAAGAATTGTGGGAAGCTTTGGTTTTGTATCTGTGGTTCTGATTTCAAACACAAAAGATCACTAAAAGATCACGTTCGTGCGTTTGGTAATGGCCATGCTCCCCACACATTGGAATCTTTGAGTTTTGATGGACATAAAGTAAATGAAAATTACATGGAAGAAGAAGAAAGTGGAAGTGAAGATGAAGACAATAACAGTGAAGTTGTTTTTAGTTTGAATTAA
Protein sequence

>MS.gene60863.t1

MQTHHQYYSFSCESASCSSSLDDEDSIFLSLAPPGHQIPPKLAPPHPTITTTIPPLHQIPISTTTSLHHDHTCAVNVALNALINPTFTSKPSHTPIIPQYWIPSPAQILVGPTQFSCTVCNKTFNRFNNMQMHMWGHGSQYRKGPESLKGPKPASSSSMLKLPCYCCAEGCRNNINYPRARPLKDFRTLQTHYKRKHGGKSFECRKCRKTFAVRGDWRTHEKNCGKLWFCICGSDFKHKRSLKDHVRAFGNGHAPHTLESLSFDGHKVNENYMEEEESGSEDEDNNSEVVFSLN