AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene61061

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61061.t1 MTR_8g065740 97.216 467 13 0 1 467 1 467 0.0 936
MS.gene61061.t1 MTR_5g048860 57.605 526 148 13 1 459 1 518 4.89e-175 503
MS.gene61061.t1 MTR_1g017350 42.345 307 153 7 114 420 92 374 2.31e-56 193
MS.gene61061.t1 MTR_1g017350 42.345 307 153 7 114 420 92 374 3.21e-56 192
MS.gene61061.t1 MTR_1g017350 41.379 319 147 9 114 415 92 387 1.16e-53 186
MS.gene61061.t1 MTR_1g017350 41.379 319 147 9 114 415 92 387 1.18e-53 186
MS.gene61061.t1 MTR_7g053410 38.919 370 186 12 125 467 128 484 6.11e-52 183
MS.gene61061.t1 MTR_7g053410 38.919 370 186 12 125 467 128 484 7.88e-52 183
MS.gene61061.t1 MTR_3g116770 43.774 265 114 7 134 392 43 278 3.12e-51 179
MS.gene61061.t1 MTR_3g116770 43.774 265 114 7 134 392 43 278 3.47e-51 178
MS.gene61061.t1 MTR_8g012290 39.053 338 159 15 126 430 93 416 2.22e-48 173
MS.gene61061.t1 MTR_8g012290 39.053 338 159 15 126 430 93 416 2.48e-48 173
MS.gene61061.t1 MTR_4g070320 71.963 107 28 1 288 392 124 230 1.03e-45 162
MS.gene61061.t1 MTR_8g062240 50.588 170 64 3 239 394 77 240 1.51e-41 149
MS.gene61061.t1 MTR_3g498825 85.366 82 12 0 296 377 138 219 1.08e-40 149
MS.gene61061.t1 MTR_5g037250 63.115 122 43 2 283 404 91 210 1.54e-40 145
MS.gene61061.t1 MTR_8g099880 82.353 85 14 1 301 384 141 225 3.39e-40 147
MS.gene61061.t1 MTR_7g092510 73.034 89 24 0 302 390 170 258 2.10e-39 145
MS.gene61061.t1 MTR_1g059270 68.085 94 30 0 302 395 157 250 7.34e-39 141
MS.gene61061.t1 MTR_1g059270 72.093 86 24 0 302 387 157 242 1.17e-37 140
MS.gene61061.t1 MTR_6g084120 52.252 111 42 2 276 384 124 225 2.87e-26 107
MS.gene61061.t1 MTR_4g131160 64.935 77 26 1 294 370 188 263 1.25e-21 97.1
MS.gene61061.t1 MTR_4g131160 64.935 77 26 1 294 370 185 260 1.27e-21 97.1
MS.gene61061.t1 MTR_1g080890 68.182 66 20 1 305 370 193 257 4.21e-20 92.0
MS.gene61061.t1 MTR_4g033295 55.385 65 29 0 305 369 118 182 2.33e-15 74.7
MS.gene61061.t1 MTR_4g065870 58.462 65 27 0 305 369 355 419 2.70e-15 78.2
MS.gene61061.t1 MTR_4g033295 53.731 67 30 1 305 370 118 184 3.98e-15 73.9
MS.gene61061.t1 MTR_2g098310 50.704 71 35 0 305 375 332 402 6.48e-15 76.6
MS.gene61061.t1 MTR_4g065870 58.065 62 26 0 305 366 355 416 1.40e-14 75.9
MS.gene61061.t1 MTR_2g098310 56.250 64 28 0 305 368 332 395 2.13e-14 75.1
MS.gene61061.t1 MTR_1g052470 52.174 69 33 0 305 373 298 366 2.95e-14 74.7
MS.gene61061.t1 MTR_7g092540 50.725 69 34 0 305 373 243 311 3.54e-14 73.6
MS.gene61061.t1 MTR_1g084980 33.125 160 82 5 243 401 415 550 4.47e-14 75.1
MS.gene61061.t1 MTR_1g069155 54.688 64 28 1 307 370 319 381 4.49e-14 74.7
MS.gene61061.t1 MTR_1g070870 63.636 66 23 1 305 370 120 184 1.33e-13 71.6
MS.gene61061.t1 MTR_1g070870 63.636 66 23 1 305 370 123 187 1.47e-13 71.2
MS.gene61061.t1 MTR_7g092540 50.769 65 32 0 305 369 243 307 1.74e-13 71.6
MS.gene61061.t1 MTR_3g028520 50.769 65 32 0 305 369 322 386 8.62e-13 70.1
MS.gene61061.t1 MTR_3g027650 50.769 65 32 0 305 369 322 386 8.62e-13 70.1
MS.gene61061.t1 MTR_7g099540 51.562 64 30 1 307 370 254 316 1.70e-12 69.7
MS.gene61061.t1 MTR_1g019240 43.210 81 40 2 291 371 76 150 2.04e-12 67.8
MS.gene61061.t1 MTR_3g027650 51.613 62 30 0 305 366 322 383 5.11e-12 67.8
MS.gene61061.t1 MTR_8g036235 47.692 65 34 0 305 369 316 380 8.08e-12 67.0
MS.gene61061.t1 MTR_1g018090 50.769 65 31 1 305 369 274 337 1.96e-11 65.9
MS.gene61061.t1 MTR_7g099540 48.438 64 32 1 307 370 254 316 3.98e-11 65.5
MS.gene61061.t1 MTR_3g449770 50.000 64 31 1 307 370 311 373 3.98e-11 65.5
MS.gene61061.t1 MTR_5g017040 53.571 56 25 1 316 371 116 170 6.43e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61061.t1 AT1G68920 44.371 453 198 15 17 454 28 441 9.65e-97 300
MS.gene61061.t1 AT1G68920 44.371 453 198 15 17 454 28 441 9.65e-97 300
MS.gene61061.t1 AT1G68920 44.371 453 198 15 17 454 28 441 9.65e-97 300
MS.gene61061.t1 AT1G68920 44.371 453 197 15 17 454 28 440 1.28e-96 300
MS.gene61061.t1 AT1G26260 38.926 447 172 15 1 440 1 353 1.33e-69 226
MS.gene61061.t1 AT1G26260 38.926 447 172 15 1 440 1 353 1.33e-69 226
MS.gene61061.t1 AT1G26260 38.926 447 172 15 1 440 1 353 1.33e-69 226
MS.gene61061.t1 AT1G26260 38.926 447 172 15 1 440 1 353 1.33e-69 226
MS.gene61061.t1 AT1G26260 46.006 313 122 9 134 440 60 331 4.32e-66 217
MS.gene61061.t1 AT1G26260 52.137 234 85 4 207 440 96 302 3.91e-64 211
MS.gene61061.t1 AT1G10120 52.083 192 82 2 213 404 124 305 4.72e-54 185
MS.gene61061.t1 AT1G10120 52.083 192 82 2 213 404 124 305 4.72e-54 185
MS.gene61061.t1 AT3G07340 42.910 268 98 8 126 392 132 345 5.32e-49 174
MS.gene61061.t1 AT3G07340 42.910 268 98 8 126 392 132 345 7.78e-49 174
MS.gene61061.t1 AT5G48560 40.741 270 129 8 126 392 148 389 1.80e-47 170
MS.gene61061.t1 AT5G48560 40.741 270 129 8 126 392 148 389 3.98e-47 170
MS.gene61061.t1 AT4G34530 68.750 112 30 2 302 408 169 280 8.31e-45 160
MS.gene61061.t1 AT3G23690 45.022 231 70 9 262 437 109 337 1.47e-44 160
MS.gene61061.t1 AT5G50915 56.410 156 49 6 260 413 106 244 3.43e-42 151
MS.gene61061.t1 AT5G50915 56.410 156 49 6 260 413 106 244 3.43e-42 151
MS.gene61061.t1 AT2G18300 54.110 146 62 1 239 384 124 264 3.58e-42 153
MS.gene61061.t1 AT5G50915 51.667 180 62 8 260 436 106 263 3.81e-42 151
MS.gene61061.t1 AT5G50915 51.667 180 62 8 260 436 106 263 3.81e-42 151
MS.gene61061.t1 AT2G18300 54.110 146 62 1 239 384 124 264 4.16e-42 152
MS.gene61061.t1 AT2G18300 54.110 146 62 1 239 384 124 264 8.23e-42 152
MS.gene61061.t1 AT1G59640 65.455 110 37 1 285 394 117 225 5.82e-41 147
MS.gene61061.t1 AT4G36540 51.974 152 64 2 268 410 101 252 2.30e-40 147
MS.gene61061.t1 AT1G59640 44.865 185 96 3 208 392 45 223 3.38e-40 147
MS.gene61061.t1 AT4G36540 59.200 125 48 1 268 389 101 225 2.60e-39 144
MS.gene61061.t1 AT2G42300 67.778 90 29 0 302 391 182 271 1.50e-38 141
MS.gene61061.t1 AT3G57800 73.256 86 23 0 302 387 201 286 1.17e-37 142
MS.gene61061.t1 AT2G42300 68.539 89 28 0 302 390 182 270 2.66e-37 139
MS.gene61061.t1 AT5G62610 74.074 81 20 1 296 376 145 224 1.04e-35 134
MS.gene61061.t1 AT5G62610 74.074 81 20 1 296 376 145 224 1.04e-35 134
MS.gene61061.t1 AT1G73830 61.321 106 41 0 279 384 121 226 2.06e-34 130
MS.gene61061.t1 AT1G73830 61.321 106 41 0 279 384 121 226 2.10e-34 130
MS.gene61061.t1 AT1G18400 62.264 106 35 2 303 406 143 245 2.33e-34 129
MS.gene61061.t1 AT1G25330 52.713 129 55 2 269 393 66 192 4.19e-34 128
MS.gene61061.t1 AT1G18400 74.390 82 21 0 303 384 143 224 4.28e-34 129
MS.gene61061.t1 AT3G57800 47.368 133 23 1 302 387 201 333 6.02e-29 118
MS.gene61061.t1 AT2G24260 59.091 88 33 2 283 370 15 99 6.95e-23 97.8
MS.gene61061.t1 AT4G30980 56.122 98 37 2 273 370 103 194 2.17e-22 97.8
MS.gene61061.t1 AT4G30980 56.122 98 37 2 273 370 151 242 4.20e-22 97.8
MS.gene61061.t1 AT2G24260 59.091 88 33 2 283 370 118 202 4.81e-22 97.4
MS.gene61061.t1 AT5G58010 70.149 67 19 1 304 370 98 163 4.09e-21 94.0
MS.gene61061.t1 AT2G42300 81.395 43 8 0 302 344 182 224 4.65e-17 80.5
MS.gene61061.t1 AT2G42300 81.395 43 8 0 302 344 182 224 4.65e-17 80.5
MS.gene61061.t1 AT2G42300 81.395 43 8 0 302 344 182 224 5.71e-17 80.5
MS.gene61061.t1 AT2G20180 40.909 110 57 3 261 370 241 342 4.55e-16 80.9
MS.gene61061.t1 AT2G20180 40.909 110 57 3 261 370 241 342 4.55e-16 80.9
MS.gene61061.t1 AT2G20180 40.909 110 57 3 261 370 241 342 4.55e-16 80.9
MS.gene61061.t1 AT2G20180 40.909 110 57 3 261 370 241 342 4.55e-16 80.9
MS.gene61061.t1 AT2G20180 40.909 110 57 3 261 370 170 271 4.69e-16 80.5
MS.gene61061.t1 AT2G20180 40.909 110 57 3 261 370 170 271 4.69e-16 80.5
MS.gene61061.t1 AT2G20180 40.909 110 57 3 261 370 170 271 4.69e-16 80.5
MS.gene61061.t1 AT2G20180 40.187 107 56 3 261 367 241 339 7.60e-15 76.3
MS.gene61061.t1 AT4G28790 33.562 146 82 4 274 415 236 370 9.95e-15 76.3
MS.gene61061.t1 AT1G51140 56.250 64 28 0 305 368 304 367 1.94e-14 75.1
MS.gene61061.t1 AT4G28800 34.959 123 60 3 267 388 229 332 5.36e-14 74.3
MS.gene61061.t1 AT1G35460 52.308 65 31 0 305 369 181 245 5.86e-14 72.4
MS.gene61061.t1 AT4G09180 51.515 66 32 0 304 369 183 248 6.70e-14 72.0
MS.gene61061.t1 AT2G42280 50.769 65 32 0 305 369 279 343 1.05e-13 72.8
MS.gene61061.t1 AT1G09530 51.562 64 30 1 307 370 339 401 1.13e-13 73.6
MS.gene61061.t1 AT1G09530 51.562 64 30 1 307 370 339 401 1.13e-13 73.6
MS.gene61061.t1 AT1G09530 51.562 64 30 1 307 370 339 401 1.13e-13 73.6
MS.gene61061.t1 AT1G09530 51.562 64 30 1 307 370 339 401 1.13e-13 73.6
MS.gene61061.t1 AT1G09530 51.562 64 30 1 307 370 339 401 1.13e-13 73.6
MS.gene61061.t1 AT1G09530 51.562 64 30 1 307 370 339 401 1.13e-13 73.6
MS.gene61061.t1 AT4G28815 56.897 58 24 1 308 365 71 127 2.05e-13 70.1
MS.gene61061.t1 AT2G42280 53.030 66 29 1 305 368 279 344 2.21e-13 71.6
MS.gene61061.t1 AT4G28800 34.375 128 59 3 267 388 241 349 2.87e-13 72.0
MS.gene61061.t1 AT4G02590 62.121 66 24 1 305 370 83 147 4.41e-13 69.3
MS.gene61061.t1 AT4G28815 56.897 58 24 1 308 365 147 203 6.37e-13 69.7
MS.gene61061.t1 AT4G02590 62.121 66 24 1 305 370 146 210 6.55e-13 69.7
MS.gene61061.t1 AT4G02590 62.121 66 24 1 305 370 146 210 6.55e-13 69.7
MS.gene61061.t1 AT1G03040 62.121 66 24 1 305 370 139 203 7.41e-13 69.7
MS.gene61061.t1 AT1G03040 62.121 66 24 1 305 370 144 208 8.93e-13 69.3
MS.gene61061.t1 AT1G03040 62.121 66 24 1 305 370 144 208 1.63e-12 68.9
MS.gene61061.t1 AT4G28790 34.951 103 62 2 274 372 236 337 3.22e-12 68.2
MS.gene61061.t1 AT1G27740 37.398 123 60 4 263 369 109 230 5.44e-12 66.2
MS.gene61061.t1 AT4G00050 49.275 69 34 1 301 369 101 168 5.78e-12 67.0
MS.gene61061.t1 AT2G46970 34.513 113 68 2 303 410 185 296 5.91e-12 67.8
MS.gene61061.t1 AT2G46970 34.513 113 68 2 303 410 216 327 5.98e-12 67.8
MS.gene61061.t1 AT2G46970 34.513 113 68 2 303 410 194 305 7.63e-12 67.4
MS.gene61061.t1 AT4G00050 49.275 69 34 1 301 369 203 270 1.34e-11 66.6
MS.gene61061.t1 AT4G00050 49.275 69 34 1 301 369 203 270 1.64e-11 66.2
MS.gene61061.t1 AT2G43140 50.000 64 32 0 305 368 231 294 3.34e-11 64.7
MS.gene61061.t1 AT2G43140 50.000 64 32 0 305 368 134 197 3.78e-11 63.2
MS.gene61061.t1 AT4G33880 49.231 65 32 1 305 369 266 329 4.86e-11 64.7
MS.gene61061.t1 AT4G36930 50.820 61 29 1 311 371 197 256 6.95e-11 64.3

Find 126 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATATGGTCCTAACTTCT+TGG 0.275845 8.1:-38473151 MS.gene61061:CDS
TATGAATCCACCATTGAATT+AGG 0.277485 8.1:+38473168 None:intergenic
TGCAATGGATTAACCTGATT+TGG 0.278397 8.1:+38470618 None:intergenic
AGTGGAGAACCTTCTATTAA+AGG 0.279819 8.1:-38472625 MS.gene61061:CDS
GACTAGATCCGTACATCTTT+AGG 0.285853 8.1:+38470917 None:intergenic
GCTTCAACAACCAAGGCTTC+TGG 0.303821 8.1:-38471988 MS.gene61061:CDS
AAAATCCACATGTGGATTTA+CGG 0.315802 8.1:+38470958 None:intergenic
CACTTGCACAATCCAACATT+TGG 0.316306 8.1:+38472644 None:intergenic
CCTTCATCTTGAGACATTAC+AGG 0.318475 8.1:+38472733 None:intergenic
GCTCTCACAGTTGTCCTTTG+TGG 0.319104 8.1:+38472035 None:intergenic
TGCAAATTCATCTGTAGGTT+TGG 0.339897 8.1:-38473229 MS.gene61061:CDS
ACCGGGTCCATCATCTGCTC+TGG 0.343461 8.1:-38470799 MS.gene61061:CDS
GGATTTAGACCTTTAATAGA+AGG 0.344206 8.1:+38472616 None:intergenic
GAAAGGAGGGTCCTTTAGAT+TGG 0.355470 8.1:-38473272 MS.gene61061:CDS
TCAACCACAGTTGGAACCTT+TGG 0.357269 8.1:-38470658 MS.gene61061:CDS
TCTGATAAATTTGCCATGTT+AGG 0.358693 8.1:+38470699 None:intergenic
ATGTGGGATTCCTCAATCTA+TGG 0.359558 8.1:-38472839 MS.gene61061:CDS
TTCTGGGAAGAATGCCAAAC+AGG 0.361984 8.1:-38471971 MS.gene61061:CDS
TCAGATTCTTCAACATCAAC+CGG 0.371564 8.1:-38470817 MS.gene61061:intron
TCTTACTAACTCTGAAAACT+TGG 0.374246 8.1:-38473124 MS.gene61061:CDS
AATCACTTCTTCGATCAATC+TGG 0.381385 8.1:+38472758 None:intergenic
TTCTTCATGTCCTAATTCAA+TGG 0.389007 8.1:-38473178 MS.gene61061:CDS
GGCATTCTTCCCAGAAGCCT+TGG 0.393219 8.1:+38471978 None:intergenic
TTACTCTCTCTGCAAGACTA+TGG 0.395915 8.1:+38471875 None:intergenic
ATCTGTAGGTTTGGTTTCCA+TGG 0.397148 8.1:-38473220 MS.gene61061:CDS
CTTCAACAACCAAGGCTTCT+GGG 0.404138 8.1:-38471987 MS.gene61061:CDS
TTCCATGGAAAATTCTATGA+TGG 0.411759 8.1:-38473205 MS.gene61061:CDS
GGTTGATATGAATGTGATAA+TGG 0.412278 8.1:+38470744 None:intergenic
GTTGAGGAAATTTCAGTCCT+TGG 0.419505 8.1:+38470590 None:intergenic
GTAAGAAGGTCCAAGAAGTT+AGG 0.420123 8.1:+38473141 None:intergenic
CAGTCCTTGGCTGCTTGCAA+TGG 0.420935 8.1:+38470603 None:intergenic
CTTCTTCGATCAATCTGGAT+TGG 0.432794 8.1:+38472763 None:intergenic
CCAAAGAAAAGGAAAAGAAA+TGG 0.434507 8.1:-38472595 MS.gene61061:CDS
CCGGGTCCATCATCTGCTCT+GGG 0.439623 8.1:-38470798 MS.gene61061:CDS
AGCAATGAAGTGAGAAAAGA+TGG 0.442777 8.1:-38473075 MS.gene61061:CDS
TTATTCTGTTTGTAGGTCAC+TGG 0.450051 8.1:-38471619 MS.gene61061:intron
TCTCGGTCTGTTGAGCATCT+TGG 0.450146 8.1:-38472799 MS.gene61061:CDS
GTTGGAATGGGTTGGGACCT+TGG 0.452583 8.1:-38473015 MS.gene61061:CDS
TTAGATTGGAGGTTTGTTAG+TGG 0.455500 8.1:-38473258 MS.gene61061:CDS
ACTTGCACAATCCAACATTT+GGG 0.456863 8.1:+38472645 None:intergenic
ACTTGGTAGCTCAAGAGTTC+CGG 0.459094 8.1:+38472062 None:intergenic
GGAAACATCTCAGCCCCATT+TGG 0.462856 8.1:+38472958 None:intergenic
TTTCTTCAAGATCTTGTACC+TGG 0.462998 8.1:-38471740 MS.gene61061:CDS
AGGGTTTAAAGAGCCAAATC+AGG 0.463980 8.1:-38470631 MS.gene61061:CDS
CAAATTTATCAGATATGCTT+AGG 0.466891 8.1:-38470689 MS.gene61061:CDS
AATTCAATGGTGGATTCATA+TGG 0.475276 8.1:-38473165 MS.gene61061:CDS
GAAGTGAGAAAAGATGGGTT+TGG 0.476292 8.1:-38473069 MS.gene61061:CDS
AAGGTCTAAATCCAAAGAAA+AGG 0.482803 8.1:-38472606 MS.gene61061:CDS
CCACAGTTGGAACCTTTGGC+TGG 0.487866 8.1:-38470654 MS.gene61061:CDS
AGTTGGAACCTTTGGCTGGA+GGG 0.488222 8.1:-38470650 MS.gene61061:CDS
AAATGTACCTTGTTGCATCC+AGG 0.496384 8.1:+38471722 None:intergenic
GGAAGCTTGAGACCCCTGTT+TGG 0.497782 8.1:+38471957 None:intergenic
CTCTACCTCTACATGTGTCT+CGG 0.501570 8.1:-38472816 MS.gene61061:CDS
TAGAACAGTTGGAATGGGTT+GGG 0.502266 8.1:-38473022 MS.gene61061:CDS
AGGAGGGTCCTTTAGATTGG+AGG 0.508576 8.1:-38473269 MS.gene61061:CDS
TCCATGGAAAATTCTATGAT+GGG 0.513525 8.1:-38473204 MS.gene61061:CDS
GAAAAGGAAAAGAAATGGAC+AGG 0.513621 8.1:-38472590 MS.gene61061:intron
TTGTCCTTTGTGGTTTCACT+TGG 0.513815 8.1:+38472045 None:intergenic
GGCTATTTGTTGCTTGACCA+CGG 0.515569 8.1:+38471896 None:intergenic
CGATGATGGTTCCCAAATGT+TGG 0.518846 8.1:-38472656 MS.gene61061:CDS
CCTGTAATGTCTCAAGATGA+AGG 0.522379 8.1:-38472733 MS.gene61061:CDS
AGAAACCCCAGAGCAGATGA+TGG 0.522386 8.1:+38470792 None:intergenic
GTATCCCTCGTTAGGCGGAT+CGG 0.530629 8.1:+38471936 None:intergenic
GCAGACTGTTCAACCACAGT+TGG 0.533598 8.1:-38470667 MS.gene61061:CDS
CTAACAAACCTCCAATCTAA+AGG 0.535698 8.1:+38473261 None:intergenic
CTGGATTTGTTGATGCTGCA+AGG 0.542019 8.1:-38472906 MS.gene61061:CDS
TGATGATAGAACAGTTGGAA+TGG 0.544564 8.1:-38473028 MS.gene61061:CDS
ATAGAACAGTTGGAATGGGT+TGG 0.546913 8.1:-38473023 MS.gene61061:CDS
AACCTTGCAAATTCATCTGT+AGG 0.548963 8.1:-38473234 MS.gene61061:CDS
TAATCCATTGCAAGCAGCCA+AGG 0.549245 8.1:-38470607 MS.gene61061:CDS
GTTGAGCATCTTGGTAGTGA+TGG 0.549328 8.1:-38472790 MS.gene61061:CDS
GATGAAGGGAAACAAGCTCC+TGG 0.551064 8.1:-38472718 MS.gene61061:CDS
TCTTTAAACCCTCCAGCCAA+AGG 0.552371 8.1:+38470642 None:intergenic
GGTTGTGATGATAGAACAGT+TGG 0.552863 8.1:-38473033 MS.gene61061:CDS
CCCAGAGCAGATGATGGACC+CGG 0.555611 8.1:+38470798 None:intergenic
TTCATGTCCTAATTCAATGG+TGG 0.556761 8.1:-38473175 MS.gene61061:CDS
CAGTTGGAACCTTTGGCTGG+AGG 0.557432 8.1:-38470651 MS.gene61061:CDS
GATGATAGAACAGTTGGAAT+GGG 0.563426 8.1:-38473027 MS.gene61061:CDS
ACTAACTCTGAAAACTTGGG+TGG 0.566336 8.1:-38473120 MS.gene61061:CDS
GATGAATCGAGTGGCGATGA+TGG 0.566803 8.1:-38472670 MS.gene61061:CDS
GTAGTAGTTATATGATGAAG+AGG 0.571058 8.1:-38472993 MS.gene61061:CDS
AGAGGTAGAGCCATAGATTG+AGG 0.574801 8.1:+38472829 None:intergenic
CTGTAATGTCTCAAGATGAA+GGG 0.576780 8.1:-38472732 MS.gene61061:CDS
CTTACTAACTCTGAAAACTT+GGG 0.579332 8.1:-38473123 MS.gene61061:CDS
AGAGTTGAATGAATCAGTCC+TGG 0.580047 8.1:+38470723 None:intergenic
GCTTCATTACAAGACCCTCC+AGG 0.582712 8.1:+38472700 None:intergenic
CAAATGTTGGATTGTGCAAG+TGG 0.582909 8.1:-38472643 MS.gene61061:CDS
AGCTTCCGATCCGCCTAACG+AGG 0.588358 8.1:-38471941 MS.gene61061:CDS
TCTGGGAAGAATGCCAAACA+GGG 0.593622 8.1:-38471970 MS.gene61061:CDS
ACATGTACGTATCCCTCGTT+AGG 0.594600 8.1:+38471928 None:intergenic
TAGATATGATGAACTCATGT+GGG 0.599148 8.1:-38472855 MS.gene61061:CDS
CATCATATCTACGAAACTCC+CGG 0.605426 8.1:+38472867 None:intergenic
TTTGAGGTAGATAGAAAGGA+GGG 0.606702 8.1:-38473285 MS.gene61061:CDS
CTGTTTGTAGGTCACTGGCA+AGG 0.607483 8.1:-38471614 MS.gene61061:intron
TCCATATTAAAATCCACATG+TGG 0.609814 8.1:+38470950 None:intergenic
GATAATGGAGGAAAAGCCAT+TGG 0.610652 8.1:+38470759 None:intergenic
TCATCATATAACTACTACCA+AGG 0.611520 8.1:+38472998 None:intergenic
TCATTCAACTCTACCTAACA+TGG 0.611728 8.1:-38470712 MS.gene61061:CDS
AACCTACAGATGAATTTGCA+AGG 0.617947 8.1:+38473232 None:intergenic
CAGATTCTTCAACATCAACC+GGG 0.618225 8.1:-38470816 MS.gene61061:CDS
TGGTAGCTCAAGAGTTCCGG+TGG 0.619141 8.1:+38472065 None:intergenic
GCTTCCGATCCGCCTAACGA+GGG 0.622900 8.1:-38471940 MS.gene61061:CDS
TAGTAGTTATATGATGAAGA+GGG 0.623299 8.1:-38472992 MS.gene61061:CDS
CCAGCCAAAGGTTCCAACTG+TGG 0.623573 8.1:+38470654 None:intergenic
GGGTCTTGTAATGAAGCTGA+TGG 0.623907 8.1:-38472694 MS.gene61061:CDS
AAGGGAAACAAGCTCCTGGA+GGG 0.627076 8.1:-38472714 MS.gene61061:CDS
TGTACGTATCCCTCGTTAGG+CGG 0.628257 8.1:+38471931 None:intergenic
GTAGATATGATGAACTCATG+TGG 0.633019 8.1:-38472856 MS.gene61061:CDS
AGTTATATGATGAAGAGGGA+TGG 0.633166 8.1:-38472988 MS.gene61061:CDS
GTCTCTTCAACAACAAGTTG+AGG 0.634721 8.1:-38471557 MS.gene61061:intron
TGCAACCGTAAATCCACATG+TGG 0.638726 8.1:-38470963 MS.gene61061:CDS
GGTGATAGTGATAAAGCCAC+CGG 0.644324 8.1:-38472081 MS.gene61061:CDS
GCAAACAGCTTCAACAACCA+AGG 0.656445 8.1:-38471995 MS.gene61061:CDS
TGATATGAATGTGATAATGG+AGG 0.657822 8.1:+38470747 None:intergenic
GCAATGAAGTGAGAAAAGAT+GGG 0.658069 8.1:-38473074 MS.gene61061:CDS
CGGGTCCATCATCTGCTCTG+GGG 0.660359 8.1:-38470797 MS.gene61061:CDS
GTTTCTCAGAGAAATGCCAA+TGG 0.663274 8.1:-38470775 MS.gene61061:CDS
TCTTGTACCTGGATGCAACA+AGG 0.668293 8.1:-38471729 MS.gene61061:intron
ACAGACCGAGACACATGTAG+AGG 0.676355 8.1:+38472811 None:intergenic
GAAGGGAAACAAGCTCCTGG+AGG 0.678820 8.1:-38472715 MS.gene61061:CDS
GAAAACTGAGACAAACTCTG+AGG 0.709780 8.1:+38472937 None:intergenic
TTATCACATTCATATCAACC+AGG 0.710399 8.1:-38470741 MS.gene61061:CDS
GCTACCAAGTGAAACCACAA+AGG 0.714013 8.1:-38472049 MS.gene61061:CDS
CTGGGAAGAATGCCAAACAG+GGG 0.714387 8.1:-38471969 MS.gene61061:CDS
GCTGATGGAGATGAATCGAG+TGG 0.745292 8.1:-38472679 MS.gene61061:CDS
GTACATGTTAGAGCTCGCCG+TGG 0.792846 8.1:-38471913 MS.gene61061:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTATTTTATTTTCTGTT+AGG - chr8.1:38471799-38471818 MS.gene61061:intron 10.0%
!! AAAAAAAATTGTTTGGATTT+AGG - chr8.1:38472081-38472100 MS.gene61061:CDS 15.0%
!! AAAATTCATCATAGTAAATT+GGG - chr8.1:38471621-38471640 MS.gene61061:intron 15.0%
!! TAAAATAAAATAAACTCACA+TGG + chr8.1:38471785-38471804 None:intergenic 15.0%
!!! ATACAATAAATTGTTTTGTT+AGG - chr8.1:38472813-38472832 MS.gene61061:CDS 15.0%
!!! ATTTCTAGAAACATATATAA+GGG + chr8.1:38472855-38472874 None:intergenic 15.0%
!!! GTAAAATCATTAGAAATTTT+AGG - chr8.1:38471486-38471505 MS.gene61061:intron 15.0%
!!! TACAATAAATTGTTTTGTTA+GGG - chr8.1:38472814-38472833 MS.gene61061:CDS 15.0%
!! AAAAAATTGGTATTGTTTAG+CGG + chr8.1:38472568-38472587 None:intergenic 20.0%
!! AAATTATTGCAATTTCAAAC+AGG + chr8.1:38472722-38472741 None:intergenic 20.0%
!! ATAGCAGAATTTGAAAAAAT+TGG + chr8.1:38472581-38472600 None:intergenic 20.0%
!! ATTATTTAGATTTCAACTGT+AGG - chr8.1:38472110-38472129 MS.gene61061:intron 20.0%
!! CAAAATTCATCATAGTAAAT+TGG - chr8.1:38471620-38471639 MS.gene61061:intron 20.0%
!! TAAATCCTCATATGAATATA+AGG + chr8.1:38472237-38472256 None:intergenic 20.0%
!! TGATGAGAAAAAAAATTGTT+TGG - chr8.1:38472074-38472093 MS.gene61061:CDS 20.0%
!!! ACAATAAATTGTTTTGTTAG+GGG - chr8.1:38472815-38472834 MS.gene61061:CDS 20.0%
!!! CAAAATAGTCAATTTTATGA+AGG + chr8.1:38473068-38473087 None:intergenic 20.0%
!!! GATTTCTAGAAACATATATA+AGG + chr8.1:38472856-38472875 None:intergenic 20.0%
!!! TATTTTATTTTCTGTTAGGA+TGG - chr8.1:38471803-38471822 MS.gene61061:intron 20.0%
! AAAAATATGGGAAAATGAGT+TGG + chr8.1:38472416-38472435 None:intergenic 25.0%
! ATATCCAAAATAGATACACT+AGG - chr8.1:38471660-38471679 MS.gene61061:intron 25.0%
! ATCTTTGTTACAATTGGTAA+AGG - chr8.1:38472777-38472796 MS.gene61061:CDS 25.0%
! CAAATTTATCAGATATGCTT+AGG - chr8.1:38473216-38473235 MS.gene61061:CDS 25.0%
! GTAAATTGGGATTAAAAATG+AGG - chr8.1:38471634-38471653 MS.gene61061:intron 25.0%
! GTCATTAATCAAATGACAAA+AGG - chr8.1:38471547-38471566 MS.gene61061:intron 25.0%
! TAGTAGTTATATGATGAAGA+GGG - chr8.1:38470913-38470932 MS.gene61061:intron 25.0%
! TATCCAAAATAGATACACTA+GGG - chr8.1:38471661-38471680 MS.gene61061:intron 25.0%
! TTGGATGATTTAATAATCAG+TGG + chr8.1:38472397-38472416 None:intergenic 25.0%
!! AACTCATTTTCCCATATTTT+TGG - chr8.1:38472415-38472434 MS.gene61061:intron 25.0%
!! ATCATTGTTATTCTGTTTGT+AGG - chr8.1:38472279-38472298 MS.gene61061:intron 25.0%
!! TAACTGTTATTTGACAACAT+AGG - chr8.1:38472623-38472642 MS.gene61061:CDS 25.0%
!! TTGTCATTTGATTAATGACT+TGG + chr8.1:38471546-38471565 None:intergenic 25.0%
!! TTTAGTTTCAATATAGTCGT+AGG - chr8.1:38471519-38471538 MS.gene61061:intron 25.0%
!!! AAATTTTGAGGTAGATAGAA+AGG - chr8.1:38470616-38470635 MS.gene61061:CDS 25.0%
!!! ATGTGGATTTTAATATGGAA+CGG - chr8.1:38472959-38472978 MS.gene61061:CDS 25.0%
!!! ATTGTAAGCTTTAAAATTGC+TGG + chr8.1:38473024-38473043 None:intergenic 25.0%
!!! TTTTAGTATAGTTGATACTG+TGG - chr8.1:38471358-38471377 MS.gene61061:intron 25.0%
AAAATAAACTCACATGGACA+AGG + chr8.1:38471779-38471798 None:intergenic 30.0%
AAAATCCACATGTGGATTTA+CGG + chr8.1:38472950-38472969 None:intergenic 30.0%
AAATAAACTCACATGGACAA+GGG + chr8.1:38471778-38471797 None:intergenic 30.0%
AAGGTCTAAATCCAAAGAAA+AGG - chr8.1:38471299-38471318 MS.gene61061:intron 30.0%
AATTGTAACAAAGATGACAC+TGG + chr8.1:38472772-38472791 None:intergenic 30.0%
AGTGTCATCTTTGTTACAAT+TGG - chr8.1:38472771-38472790 MS.gene61061:CDS 30.0%
ATACTGTGGTTATCAATTTG+AGG - chr8.1:38471372-38471391 MS.gene61061:intron 30.0%
ATGTTAATGAGAAACTCTAC+TGG + chr8.1:38472674-38472693 None:intergenic 30.0%
ATTGATAAAAACTGCCAAGA+GGG + chr8.1:38472916-38472935 None:intergenic 30.0%
CACTACCTTATATTCATATG+AGG - chr8.1:38472229-38472248 MS.gene61061:intron 30.0%
CCAAAGAAAAGGAAAAGAAA+TGG - chr8.1:38471310-38471329 MS.gene61061:intron 30.0%
GAAGCAGAATCCAAAAATAT+GGG + chr8.1:38472428-38472447 None:intergenic 30.0%
GGATTTAGACCTTTAATAGA+AGG + chr8.1:38471292-38471311 None:intergenic 30.0%
GTAGTAGTTATATGATGAAG+AGG - chr8.1:38470912-38470931 MS.gene61061:intron 30.0%
TAGATATGATGAACTCATGT+GGG - chr8.1:38471050-38471069 MS.gene61061:intron 30.0%
TATGAATCCACCATTGAATT+AGG + chr8.1:38470740-38470759 None:intergenic 30.0%
TCATCATATAACTACTACCA+AGG + chr8.1:38470910-38470929 None:intergenic 30.0%
TCCATATTAAAATCCACATG+TGG + chr8.1:38472958-38472977 None:intergenic 30.0%
TCCATGGAAAATTCTATGAT+GGG - chr8.1:38470701-38470720 MS.gene61061:CDS 30.0%
TCTGATAAATTTGCCATGTT+AGG + chr8.1:38473209-38473228 None:intergenic 30.0%
TGAAGCAGAATCCAAAAATA+TGG + chr8.1:38472429-38472448 None:intergenic 30.0%
TTATCACATTCATATCAACC+AGG - chr8.1:38473164-38473183 MS.gene61061:CDS 30.0%
TTCCATGGAAAATTCTATGA+TGG - chr8.1:38470700-38470719 MS.gene61061:CDS 30.0%
TTCTTCATGTCCTAATTCAA+TGG - chr8.1:38470727-38470746 MS.gene61061:CDS 30.0%
TTGAGGTTCAAATCGTTAAT+CGG - chr8.1:38471389-38471408 MS.gene61061:intron 30.0%
! AATCCCTAGTGTATCTATTT+TGG + chr8.1:38471667-38471686 None:intergenic 30.0%
! ACAATCTATTTTCTGAATCG+TGG - chr8.1:38471412-38471431 MS.gene61061:intron 30.0%
! ACTAGAGCTACACTAATTTT+AGG - chr8.1:38471576-38471595 MS.gene61061:CDS 30.0%
! AGTTTTCAGAGTTAGTAAGA+AGG + chr8.1:38470781-38470800 None:intergenic 30.0%
! CCATTTCTTTTCCTTTTCTT+TGG + chr8.1:38471313-38471332 None:intergenic 30.0%
! CTTACTAACTCTGAAAACTT+GGG - chr8.1:38470782-38470801 MS.gene61061:CDS 30.0%
! GAGTGAAAGTGAAAATTTTG+AGG - chr8.1:38470604-38470623 MS.gene61061:CDS 30.0%
! GGTTGATATGAATGTGATAA+TGG + chr8.1:38473164-38473183 None:intergenic 30.0%
! GTACATTTAAAACTTGAGAC+TGG - chr8.1:38472198-38472217 MS.gene61061:intron 30.0%
! TCTTACTAACTCTGAAAACT+TGG - chr8.1:38470781-38470800 MS.gene61061:CDS 30.0%
!! AATTCAATGGTGGATTCATA+TGG - chr8.1:38470740-38470759 MS.gene61061:CDS 30.0%
!! TCCACATGTGGATTTTAATA+TGG - chr8.1:38472954-38472973 MS.gene61061:CDS 30.0%
!! TGATATGAATGTGATAATGG+AGG + chr8.1:38473161-38473180 None:intergenic 30.0%
AACCTACAGATGAATTTGCA+AGG + chr8.1:38470676-38470695 None:intergenic 35.0%
AACCTTGCAAATTCATCTGT+AGG - chr8.1:38470671-38470690 MS.gene61061:CDS 35.0%
AACTGTGAGAGCAGAAAAAA+GGG - chr8.1:38471881-38471900 MS.gene61061:CDS 35.0%
AATAAACTCACATGGACAAG+GGG + chr8.1:38471777-38471796 None:intergenic 35.0%
AATCACTTCTTCGATCAATC+TGG + chr8.1:38471150-38471169 None:intergenic 35.0%
ACTTGCACAATCCAACATTT+GGG + chr8.1:38471263-38471282 None:intergenic 35.0%
AGCAATGAAGTGAGAAAAGA+TGG - chr8.1:38470830-38470849 MS.gene61061:intron 35.0%
AGTGGAGAACCTTCTATTAA+AGG - chr8.1:38471280-38471299 MS.gene61061:intron 35.0%
AGTTATATGATGAAGAGGGA+TGG - chr8.1:38470917-38470936 MS.gene61061:intron 35.0%
ATATAAGGGACAACTGACAA+AGG + chr8.1:38472841-38472860 None:intergenic 35.0%
CATTGATAAAAACTGCCAAG+AGG + chr8.1:38472917-38472936 None:intergenic 35.0%
CTAACAAACCTCCAATCTAA+AGG + chr8.1:38470647-38470666 None:intergenic 35.0%
CTGTAATGTCTCAAGATGAA+GGG - chr8.1:38471173-38471192 MS.gene61061:intron 35.0%
GAAAAGGAAAAGAAATGGAC+AGG - chr8.1:38471315-38471334 MS.gene61061:intron 35.0%
GATGATAGAACAGTTGGAAT+GGG - chr8.1:38470878-38470897 MS.gene61061:intron 35.0%
GCAATGAAGTGAGAAAAGAT+GGG - chr8.1:38470831-38470850 MS.gene61061:intron 35.0%
GTAGATATGATGAACTCATG+TGG - chr8.1:38471049-38471068 MS.gene61061:intron 35.0%
TCAGATTCTTCAACATCAAC+CGG - chr8.1:38473088-38473107 MS.gene61061:CDS 35.0%
TCATTCAACTCTACCTAACA+TGG - chr8.1:38473193-38473212 MS.gene61061:CDS 35.0%
TGATGATAGAACAGTTGGAA+TGG - chr8.1:38470877-38470896 MS.gene61061:intron 35.0%
TGCAAATTCATCTGTAGGTT+TGG - chr8.1:38470676-38470695 MS.gene61061:CDS 35.0%
TTAGATTTCAACTGTAGGTG+AGG - chr8.1:38472115-38472134 MS.gene61061:intron 35.0%
TTATTCTGTTTGTAGGTCAC+TGG - chr8.1:38472286-38472305 MS.gene61061:intron 35.0%
TTCATATGGTCCTAACTTCT+TGG - chr8.1:38470754-38470773 MS.gene61061:CDS 35.0%
TTCATGTCCTAATTCAATGG+TGG - chr8.1:38470730-38470749 MS.gene61061:CDS 35.0%
TTTCTTCAAGATCTTGTACC+TGG - chr8.1:38472165-38472184 MS.gene61061:intron 35.0%
! ACCCATCATAGAATTTTCCA+TGG + chr8.1:38470705-38470724 None:intergenic 35.0%
! CAATTTTATGAAGGTCAGTC+AGG + chr8.1:38473059-38473078 None:intergenic 35.0%
! GGTGGTTTTTGTGATGTTAA+TGG - chr8.1:38470803-38470822 MS.gene61061:CDS 35.0%
! TTCAACAACAAGTTGAGGTA+TGG - chr8.1:38472353-38472372 MS.gene61061:intron 35.0%
! TTTGAGGTAGATAGAAAGGA+GGG - chr8.1:38470620-38470639 MS.gene61061:CDS 35.0%
!! TCTCAGTTTTCAACTGATTC+TGG - chr8.1:38470980-38470999 MS.gene61061:CDS 35.0%
!! TGCAATGGATTAACCTGATT+TGG + chr8.1:38473290-38473309 None:intergenic 35.0%
!! TTAGATTGGAGGTTTGTTAG+TGG - chr8.1:38470647-38470666 MS.gene61061:CDS 35.0%
!!! TTTTGAGGTAGATAGAAAGG+AGG - chr8.1:38470619-38470638 MS.gene61061:CDS 35.0%
AAATGTACCTTGTTGCATCC+AGG + chr8.1:38472186-38472205 None:intergenic 40.0%
AGAGTTGAATGAATCAGTCC+TGG + chr8.1:38473185-38473204 None:intergenic 40.0%
AGGGTTTAAAGAGCCAAATC+AGG - chr8.1:38473274-38473293 MS.gene61061:CDS 40.0%
ATAAACTCACATGGACAAGG+GGG + chr8.1:38471776-38471795 None:intergenic 40.0%
ATAGAACAGTTGGAATGGGT+TGG - chr8.1:38470882-38470901 MS.gene61061:intron 40.0%
ATGTGGGATTCCTCAATCTA+TGG - chr8.1:38471066-38471085 MS.gene61061:intron 40.0%
CAAATGTTGGATTGTGCAAG+TGG - chr8.1:38471262-38471281 MS.gene61061:intron 40.0%
CAACTGTGAGAGCAGAAAAA+AGG - chr8.1:38471880-38471899 MS.gene61061:CDS 40.0%
CACTTGCACAATCCAACATT+TGG + chr8.1:38471264-38471283 None:intergenic 40.0%
CAGATTCTTCAACATCAACC+GGG - chr8.1:38473089-38473108 MS.gene61061:CDS 40.0%
CATCATATCTACGAAACTCC+CGG + chr8.1:38471041-38471060 None:intergenic 40.0%
CCTGTAATGTCTCAAGATGA+AGG - chr8.1:38471172-38471191 MS.gene61061:intron 40.0%
CCTTCATCTTGAGACATTAC+AGG + chr8.1:38471175-38471194 None:intergenic 40.0%
CTTCTTCGATCAATCTGGAT+TGG + chr8.1:38471145-38471164 None:intergenic 40.0%
GAAAACTGAGACAAACTCTG+AGG + chr8.1:38470971-38470990 None:intergenic 40.0%
GACTAGATCCGTACATCTTT+AGG + chr8.1:38472991-38473010 None:intergenic 40.0%
GGGGGAAACAGTATAAATCA+TGG + chr8.1:38471758-38471777 None:intergenic 40.0%
GGTTGTGATGATAGAACAGT+TGG - chr8.1:38470872-38470891 MS.gene61061:intron 40.0%
GTAAGAAGGTCCAAGAAGTT+AGG + chr8.1:38470767-38470786 None:intergenic 40.0%
GTCTCTTCAACAACAAGTTG+AGG - chr8.1:38472348-38472367 MS.gene61061:intron 40.0%
GTTTCTCAGAGAAATGCCAA+TGG - chr8.1:38473130-38473149 MS.gene61061:CDS 40.0%
TATAGCACCAATAGCGCAAA+TGG + chr8.1:38472605-38472624 None:intergenic 40.0%
TTACTCTCTCTGCAAGACTA+TGG + chr8.1:38472033-38472052 None:intergenic 40.0%
TTGTCCTTTGTGGTTTCACT+TGG + chr8.1:38471863-38471882 None:intergenic 40.0%
TTGTTTAGCGGTACACTTAG+AGG + chr8.1:38472556-38472575 None:intergenic 40.0%
! ACTAACTCTGAAAACTTGGG+TGG - chr8.1:38470785-38470804 MS.gene61061:CDS 40.0%
! AGGGATGGTGTTTTACCAAA+TGG - chr8.1:38470932-38470951 MS.gene61061:CDS 40.0%
! ATCTGTAGGTTTGGTTTCCA+TGG - chr8.1:38470685-38470704 MS.gene61061:CDS 40.0%
! GAAGTGAGAAAAGATGGGTT+TGG - chr8.1:38470836-38470855 MS.gene61061:intron 40.0%
! GGATGGTGTTTTACCAAATG+GGG - chr8.1:38470934-38470953 MS.gene61061:CDS 40.0%
! GGGATGGTGTTTTACCAAAT+GGG - chr8.1:38470933-38470952 MS.gene61061:CDS 40.0%
! TAGAACAGTTGGAATGGGTT+GGG - chr8.1:38470883-38470902 MS.gene61061:intron 40.0%
! TGACACATTTTTCTCCCTCT+TGG - chr8.1:38472899-38472918 MS.gene61061:CDS 40.0%
!! GATAATGGAGGAAAAGCCAT+TGG + chr8.1:38473149-38473168 None:intergenic 40.0%
!!! AGGATGTCATGTTTTAGTGC+CGG - chr8.1:38471019-38471038 MS.gene61061:intron 40.0%
ACATGTACGTATCCCTCGTT+AGG + chr8.1:38471980-38471999 None:intergenic 45.0%
AGAGGTAGAGCCATAGATTG+AGG + chr8.1:38471079-38471098 None:intergenic 45.0%
CTCTACCTCTACATGTGTCT+CGG - chr8.1:38471089-38471108 MS.gene61061:intron 45.0%
CTTCAACAACCAAGGCTTCT+GGG - chr8.1:38471918-38471937 MS.gene61061:CDS 45.0%
GAAAGGAGGGTCCTTTAGAT+TGG - chr8.1:38470633-38470652 MS.gene61061:CDS 45.0%
GCAAACAGCTTCAACAACCA+AGG - chr8.1:38471910-38471929 MS.gene61061:CDS 45.0%
GCTACCAAGTGAAACCACAA+AGG - chr8.1:38471856-38471875 MS.gene61061:intron 45.0%
GGCTGCTTCCTAAAGATGTA+CGG - chr8.1:38472980-38472999 MS.gene61061:CDS 45.0%
TAATCCATTGCAAGCAGCCA+AGG - chr8.1:38473298-38473317 MS.gene61061:CDS 45.0%
TCAACCACAGTTGGAACCTT+TGG - chr8.1:38473247-38473266 MS.gene61061:CDS 45.0%
TCTGGGAAGAATGCCAAACA+GGG - chr8.1:38471935-38471954 MS.gene61061:CDS 45.0%
TCTTGTACCTGGATGCAACA+AGG - chr8.1:38472176-38472195 MS.gene61061:intron 45.0%
TCTTTAAACCCTCCAGCCAA+AGG + chr8.1:38473266-38473285 None:intergenic 45.0%
TGCAACCGTAAATCCACATG+TGG - chr8.1:38472942-38472961 MS.gene61061:CDS 45.0%
! ACTTGGTAGCTCAAGAGTTC+CGG + chr8.1:38471846-38471865 None:intergenic 45.0%
! CGATGATGGTTCCCAAATGT+TGG - chr8.1:38471249-38471268 MS.gene61061:intron 45.0%
! GGCTATTTGTTGCTTGACCA+CGG + chr8.1:38472012-38472031 None:intergenic 45.0%
! GGGTCTTGTAATGAAGCTGA+TGG - chr8.1:38471211-38471230 MS.gene61061:intron 45.0%
! GGTGATAGTGATAAAGCCAC+CGG - chr8.1:38471824-38471843 MS.gene61061:intron 45.0%
! TGCTATGCCATTTGCGCTAT+TGG - chr8.1:38472595-38472614 MS.gene61061:CDS 45.0%
! TTCTGGGAAGAATGCCAAAC+AGG - chr8.1:38471934-38471953 MS.gene61061:CDS 45.0%
!! CTGGATTTGTTGATGCTGCA+AGG - chr8.1:38470999-38471018 MS.gene61061:intron 45.0%
!! GTTGAGCATCTTGGTAGTGA+TGG - chr8.1:38471115-38471134 MS.gene61061:intron 45.0%
!!! GGATGTCATGTTTTAGTGCC+GGG - chr8.1:38471020-38471039 MS.gene61061:intron 45.0%
!!! GGGTTTGGTTTTGCAAGAGT+TGG - chr8.1:38470851-38470870 MS.gene61061:intron 45.0%
!!! TTAAAAAATTCATATTTTAT+TGG + chr8.1:38471466-38471485 None:intergenic 5.0%
AAGGGAAACAAGCTCCTGGA+GGG - chr8.1:38471191-38471210 MS.gene61061:intron 50.0%
ACAAGTGTGCTATAGCGCTG+TGG + chr8.1:38472508-38472527 None:intergenic 50.0%
ACAGACCGAGACACATGTAG+AGG + chr8.1:38471097-38471116 None:intergenic 50.0%
AGAAACCCCAGAGCAGATGA+TGG + chr8.1:38473116-38473135 None:intergenic 50.0%
AGGAGGGTCCTTTAGATTGG+AGG - chr8.1:38470636-38470655 MS.gene61061:CDS 50.0%
AGTTGGAACCTTTGGCTGGA+GGG - chr8.1:38473255-38473274 MS.gene61061:CDS 50.0%
CTGGGAAGAATGCCAAACAG+GGG - chr8.1:38471936-38471955 MS.gene61061:CDS 50.0%
CTGTTTGTAGGTCACTGGCA+AGG - chr8.1:38472291-38472310 MS.gene61061:intron 50.0%
GATGAAGGGAAACAAGCTCC+TGG - chr8.1:38471187-38471206 MS.gene61061:intron 50.0%
GATGAATCGAGTGGCGATGA+TGG - chr8.1:38471235-38471254 MS.gene61061:intron 50.0%
GCAGACTGTTCAACCACAGT+TGG - chr8.1:38473238-38473257 MS.gene61061:CDS 50.0%
GCTATAGCGCTGTGGAATAG+TGG + chr8.1:38472500-38472519 None:intergenic 50.0%
GCTCTCACAGTTGTCCTTTG+TGG + chr8.1:38471873-38471892 None:intergenic 50.0%
GCTGATGGAGATGAATCGAG+TGG - chr8.1:38471226-38471245 MS.gene61061:intron 50.0%
GCTTCAACAACCAAGGCTTC+TGG - chr8.1:38471917-38471936 MS.gene61061:CDS 50.0%
GCTTCATTACAAGACCCTCC+AGG + chr8.1:38471208-38471227 None:intergenic 50.0%
GGAAACATCTCAGCCCCATT+TGG + chr8.1:38470950-38470969 None:intergenic 50.0%
TCTCGGTCTGTTGAGCATCT+TGG - chr8.1:38471106-38471125 MS.gene61061:intron 50.0%
TGTACGTATCCCTCGTTAGG+CGG + chr8.1:38471977-38471996 None:intergenic 50.0%
CAGTCCTTGGCTGCTTGCAA+TGG + chr8.1:38473305-38473324 None:intergenic 55.0%
CAGTTGGAACCTTTGGCTGG+AGG - chr8.1:38473254-38473273 MS.gene61061:CDS 55.0%
CCACAGTTGGAACCTTTGGC+TGG - chr8.1:38473251-38473270 MS.gene61061:CDS 55.0%
CCAGCCAAAGGTTCCAACTG+TGG + chr8.1:38473254-38473273 None:intergenic 55.0%
GAAGGGAAACAAGCTCCTGG+AGG - chr8.1:38471190-38471209 MS.gene61061:intron 55.0%
GGAAGCTTGAGACCCCTGTT+TGG + chr8.1:38471951-38471970 None:intergenic 55.0%
GGCATTCTTCCCAGAAGCCT+TGG + chr8.1:38471930-38471949 None:intergenic 55.0%
GTACATGTTAGAGCTCGCCG+TGG - chr8.1:38471992-38472011 MS.gene61061:CDS 55.0%
GTATCCCTCGTTAGGCGGAT+CGG + chr8.1:38471972-38471991 None:intergenic 55.0%
TGGTAGCTCAAGAGTTCCGG+TGG + chr8.1:38471843-38471862 None:intergenic 55.0%
!! GTTGGAATGGGTTGGGACCT+TGG - chr8.1:38470890-38470909 MS.gene61061:intron 55.0%
ACCGGGTCCATCATCTGCTC+TGG - chr8.1:38473106-38473125 MS.gene61061:CDS 60.0%
AGCTTCCGATCCGCCTAACG+AGG - chr8.1:38471964-38471983 MS.gene61061:CDS 60.0%
CCGGGTCCATCATCTGCTCT+GGG - chr8.1:38473107-38473126 MS.gene61061:CDS 60.0%
CGGGTCCATCATCTGCTCTG+GGG - chr8.1:38473108-38473127 MS.gene61061:CDS 60.0%
GCTTCCGATCCGCCTAACGA+GGG - chr8.1:38471965-38471984 MS.gene61061:CDS 60.0%
!! CCCAGAGCAGATGATGGACC+CGG + chr8.1:38473110-38473129 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 38470602 38473325 38470602 ID=MS.gene61061
chr8.1 mRNA 38470602 38473325 38470602 ID=MS.gene61061.t1;Parent=MS.gene61061
chr8.1 exon 38472591 38473325 38472591 ID=MS.gene61061.t1.exon1;Parent=MS.gene61061.t1
chr8.1 CDS 38472591 38473325 38472591 ID=cds.MS.gene61061.t1;Parent=MS.gene61061.t1
chr8.1 exon 38471879 38472106 38471879 ID=MS.gene61061.t1.exon2;Parent=MS.gene61061.t1
chr8.1 CDS 38471879 38472106 38471879 ID=cds.MS.gene61061.t1;Parent=MS.gene61061.t1
chr8.1 exon 38471730 38471795 38471730 ID=MS.gene61061.t1.exon3;Parent=MS.gene61061.t1
chr8.1 CDS 38471730 38471795 38471730 ID=cds.MS.gene61061.t1;Parent=MS.gene61061.t1
chr8.1 exon 38471558 38471626 38471558 ID=MS.gene61061.t1.exon4;Parent=MS.gene61061.t1
chr8.1 CDS 38471558 38471626 38471558 ID=cds.MS.gene61061.t1;Parent=MS.gene61061.t1
chr8.1 exon 38470931 38471002 38470931 ID=MS.gene61061.t1.exon5;Parent=MS.gene61061.t1
chr8.1 CDS 38470931 38471002 38470931 ID=cds.MS.gene61061.t1;Parent=MS.gene61061.t1
chr8.1 exon 38470602 38470835 38470602 ID=MS.gene61061.t1.exon6;Parent=MS.gene61061.t1
chr8.1 CDS 38470602 38470835 38470602 ID=cds.MS.gene61061.t1;Parent=MS.gene61061.t1
Gene Sequence

>MS.gene61061

ATGAGTGAAAGTGAAAATTTTGAGGTAGATAGAAAGGAGGGTCCTTTAGATTGGAGGTTTGTTAGTGGCAACCTTGCAAATTCATCTGTAGGTTTGGTTTCCATGGAAAATTCTATGATGGGTTCTTCTTCATGTCCTAATTCAATGGTGGATTCATATGGTCCTAACTTCTTGGACCTTCTTACTAACTCTGAAAACTTGGGTGGTTTTTGTGATGTTAATGGTCAGAGCAATGAAGTGAGAAAAGATGGGTTTGGTTTTGCAAGAGTTGGTTGTGATGATAGAACAGTTGGAATGGGTTGGGACCTTGGTAGTAGTTATATGATGAAGAGGGATGGTGTTTTACCAAATGGGGCTGAGATGTTTCCTCAGAGTTTGTCTCAGTTTTCAACTGATTCTGGATTTGTTGATGCTGCAAGGATGTCATGTTTTAGTGCCGGGAGTTTCGTAGATATGATGAACTCATGTGGGATTCCTCAATCTATGGCTCTACCTCTACATGTGTCTCGGTCTGTTGAGCATCTTGGTAGTGATGGAAGTCCAATCCAGATTGATCGAAGAAGTGATTGTCCTGTAATGTCTCAAGATGAAGGGAAACAAGCTCCTGGAGGGTCTTGTAATGAAGCTGATGGAGATGAATCGAGTGGCGATGATGGTTCCCAAATGTTGGATTGTGCAAGTGGAGAACCTTCTATTAAAGGTCTAAATCCAAAGAAAAGGAAAAGAAATGGACAGGTATATTTCTTTGAGTTGTATTTTTAGTATAGTTGATACTGTGGTTATCAATTTGAGGTTCAAATCGTTAATCGGACAATCTATTTTCTGAATCGTGGATTGAATCTTTTATTATGAATCGTAAGACCAATAAAATATGAATTTTTTAAGTAAAATCATTAGAAATTTTAGGTTGTGATTAATTTAGTTTCAATATAGTCGTAGGTCCAAGTCATTAATCAAATGACAAAAGGAAAGTGACTAGAGCTACACTAATTTTAGGTTGTGATTCATCAGAAACAATCAAAATTCATCATAGTAAATTGGGATTAAAAATGAGGTTCATATCCAAAATAGATACACTAGGGATTTGTATTGATCGAGTTTAGTTTTGTATCGAATCAAGATACAACTCACATGTATCGTAATATATTGATAGCCATGATTTATACTGTTTCCCCCTTGTCCATGTGAGTTTATTTTATTTTATTTTATTTTCTGTTAGGATGGTGATAGTGATAAAGCCACCGGAACTCTTGAGCTACCAAGTGAAACCACAAAGGACAACTGTGAGAGCAGAAAAAAGGGTAAGCAGCAAACAGCTTCAACAACCAAGGCTTCTGGGAAGAATGCCAAACAGGGGTCTCAAGCTTCCGATCCGCCTAACGAGGGATACGTACATGTTAGAGCTCGCCGTGGTCAAGCAACAAATAGCCATAGTCTTGCAGAGAGAGTAAGATGTTCTAACTAAATTTATGTGATGAGAAAAAAAATTGTTTGGATTTAGGTTTCTAATTATTTAGATTTCAACTGTAGGTGAGGAGAGAAAAGATCAGTGAAAGAATGAAGTTTCTTCAAGATCTTGTACCTGGATGCAACAAGGTACATTTAAAACTTGAGACTGGAAGATTTTCACTACCTTATATTCATATGAGGATTTAGAGTGTTGTTTTCTTTTGATATATCATTGTTATTCTGTTTGTAGGTCACTGGCAAGGCAGTGATGCTAGATGAAATCATTAATTATGTACAGTCTCTTCAACAACAAGTTGAGGTATGGCTAAAAGCATATTTCTAACCACTGATTATTAAATCATCCAACTCATTTTCCCATATTTTTGGATTCTGCTTCAGTAAGTGTTGTCAATCATGAATCAGAGAAAATAACATTTGTTTAAATTCCACTATTCCACAGCGCTATAGCACACTTGTATAGCTTCTATTTGATAACACTTGTCCTCTAAGTGTACCGCTAAACAATACCAATTTTTTCAAATTCTGCTATGCCATTTGCGCTATTGGTGCTATAACTGTTATTTGACAACATAGGCTTCTGTACGCTATCAGCATATGTTCCAGTAGAGTTTCTCATTAACATCGTTGACTGTTATAATGTTATTCTACCTGTTTGAAATTGCAATAATTTGAACGATCACTGCTGATGATTTAAATGCCAGTGTCATCTTTGTTACAATTGGTAAAGGATTACGAATAATTATACAATAAATTGTTTTGTTAGGGGCCTTTGTCAGTTGTCCCTTATATATGTTTCTAGAAATCTATGCATCTTTTTCAGTTGAAGTTGACACATTTTTCTCCCTCTTGGCAGTTTTTATCAATGAAACTTGCAACCGTAAATCCACATGTGGATTTTAATATGGAACGGCTGCTTCCTAAAGATGTACGGATCTAGTCAATTTGCATACCAGCAATTTTAAAGCTTACAATTAGCTATATATGCCTGACTGACCTTCATAAAATTGACTATTTTGTCAGATTCTTCAACATCAACCGGGTCCATCATCTGCTCTGGGGTTTCTCAGAGAAATGCCAATGGCTTTTCCTCCATTATCACATTCATATCAACCAGGACTGATTCATTCAACTCTACCTAACATGGCAAATTTATCAGATATGCTTAGGCAGACTGTTCAACCACAGTTGGAACCTTTGGCTGGAGGGTTTAAAGAGCCAAATCAGGTTAATCCATTGCAAGCAGCCAAGGACTGA
Protein sequence

>MS.gene61061.t1

MSESENFEVDRKEGPLDWRFVSGNLANSSVGLVSMENSMMGSSSCPNSMVDSYGPNFLDLLTNSENLGGFCDVNGQSNEVRKDGFGFARVGCDDRTVGMGWDLGSSYMMKRDGVLPNGAEMFPQSLSQFSTDSGFVDAARMSCFSAGSFVDMMNSCGIPQSMALPLHVSRSVEHLGSDGSPIQIDRRSDCPVMSQDEGKQAPGGSCNEADGDESSGDDGSQMLDCASGEPSIKGLNPKKRKRNGQDGDSDKATGTLELPSETTKDNCESRKKGKQQTASTTKASGKNAKQGSQASDPPNEGYVHVRARRGQATNSHSLAERVRREKISERMKFLQDLVPGCNKVTGKAVMLDEIINYVQSLQQQVEFLSMKLATVNPHVDFNMERLLPKDILQHQPGPSSALGFLREMPMAFPPLSHSYQPGLIHSTLPNMANLSDMLRQTVQPQLEPLAGGFKEPNQVNPLQAAKD