Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61120.t1 | RHN49639.1 | 69.2 | 159 | 27 | 3 | 54 | 191 | 10 | 167 | 7.90E-51 | 210.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61120.t1 | A0A396H8N5 | 69.2 | 159 | 27 | 3 | 54 | 191 | 10 | 167 | 5.7e-51 | 210.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene61120.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61120.t1 | MTR_7g117590 | 69.872 | 156 | 26 | 3 | 56 | 191 | 18 | 172 | 1.13e-68 | 207 |
| MS.gene61120.t1 | MTR_1g054470 | 48.649 | 185 | 36 | 5 | 1 | 180 | 3 | 133 | 3.62e-48 | 154 |
| MS.gene61120.t1 | MTR_2g012700 | 37.273 | 110 | 68 | 1 | 83 | 191 | 11 | 120 | 9.60e-28 | 101 |
| MS.gene61120.t1 | MTR_2g062330 | 39.316 | 117 | 66 | 3 | 76 | 191 | 27 | 139 | 1.90e-26 | 99.0 |
| MS.gene61120.t1 | MTR_5g071400 | 70.213 | 47 | 8 | 2 | 56 | 97 | 14 | 59 | 2.16e-14 | 66.2 |
| MS.gene61120.t1 | MTR_0010s0310 | 33.333 | 90 | 57 | 1 | 95 | 181 | 151 | 240 | 1.42e-13 | 67.4 |
| MS.gene61120.t1 | MTR_6g477950 | 36.275 | 102 | 61 | 3 | 90 | 190 | 46 | 144 | 3.55e-12 | 63.9 |
| MS.gene61120.t1 | MTR_6g087720 | 29.915 | 117 | 81 | 1 | 63 | 178 | 182 | 298 | 8.65e-12 | 62.8 |
| MS.gene61120.t1 | MTR_5g078270 | 31.461 | 89 | 58 | 1 | 96 | 181 | 210 | 298 | 3.46e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCATCAACAATTCGAATTT+TGG | 0.069208 | 4.4:-42711014 | None:intergenic |
| CCATCGACTTCTTCTTCATT+TGG | 0.249648 | 4.4:-42710739 | None:intergenic |
| GTGGATGGTTTGAGTTTGTT+AGG | 0.273672 | 4.4:+42711104 | MS.gene61120:CDS |
| TGAAGATCGGGTCATTTGAT+TGG | 0.360270 | 4.4:+42710418 | MS.gene61120:CDS |
| CGAATTGTTGATGATGAAAC+TGG | 0.373851 | 4.4:+42711022 | MS.gene61120:CDS |
| GAATGTGAAGTTCGTGATAA+AGG | 0.381874 | 4.4:+42711055 | MS.gene61120:CDS |
| AAGTTCGTGATAAAGGAAAT+TGG | 0.388847 | 4.4:+42711062 | MS.gene61120:CDS |
| GGCTGCTGGACGAAGAGTTC+TGG | 0.395341 | 4.4:+42710837 | MS.gene61120:CDS |
| TTTGAAATTGTGCAAATACT+TGG | 0.402550 | 4.4:-42710762 | None:intergenic |
| ATTAGTTCTGCTACGGCTGC+TGG | 0.408831 | 4.4:+42710823 | MS.gene61120:CDS |
| GAAGATCGGGTCATTTGATT+GGG | 0.427759 | 4.4:+42710419 | MS.gene61120:CDS |
| ATGAAATGTGCGGCGGATGC+AGG | 0.449012 | 4.4:+42710355 | MS.gene61120:CDS |
| CGAAGAAGAAGATGAAGATC+GGG | 0.478220 | 4.4:+42710406 | MS.gene61120:CDS |
| TGGATGGTTTGAGTTTGTTA+GGG | 0.484898 | 4.4:+42711105 | MS.gene61120:CDS |
| CTGCAGCGATGTAATTGATC+CGG | 0.509939 | 4.4:+42710684 | MS.gene61120:intron |
| CTTCTTCGTCCATCTCAAAA+CGG | 0.536060 | 4.4:-42710391 | None:intergenic |
| ACGAAGAAGAAGATGAAGAT+CGG | 0.540825 | 4.4:+42710405 | MS.gene61120:CDS |
| CGATGTAATTGATCCGGAGT+TGG | 0.544205 | 4.4:+42710690 | MS.gene61120:CDS |
| CAATCTTATTGCCGTCAAAA+CGG | 0.544280 | 4.4:-42710451 | None:intergenic |
| TTATTGCCGTCAAAACGGTT+CGG | 0.545486 | 4.4:-42710446 | None:intergenic |
| CTTTGAATTGCAATCTTCCG+AGG | 0.557056 | 4.4:-42710974 | None:intergenic |
| TCGTGATAAAGGAAATTGGA+TGG | 0.565929 | 4.4:+42711066 | MS.gene61120:CDS |
| TGAATGTGTAGCGTATTCCT+CGG | 0.575387 | 4.4:+42710957 | MS.gene61120:intron |
| AGAAATACGTTGGAAGTGGA+TGG | 0.575514 | 4.4:+42711089 | MS.gene61120:CDS |
| ATGGAGAAATACGTTGGAAG+TGG | 0.576439 | 4.4:+42711085 | MS.gene61120:CDS |
| GCATAGTATTAGTTCTGCTA+CGG | 0.580120 | 4.4:+42710816 | MS.gene61120:CDS |
| AATTGGATGGAGAAATACGT+TGG | 0.583531 | 4.4:+42711079 | MS.gene61120:CDS |
| CCATCTCAAAACGGCGACGA+TGG | 0.584489 | 4.4:-42710382 | None:intergenic |
| AGGGCAAAAGAACTCAAAGA+AGG | 0.584629 | 4.4:+42711124 | MS.gene61120:CDS |
| GCATCCGCCGCACATTTCAT+CGG | 0.590420 | 4.4:-42710352 | None:intergenic |
| GGTATCAATTCGTCCAACTC+CGG | 0.591453 | 4.4:-42710703 | None:intergenic |
| GATACAAATAATTTGTGTGG+AGG | 0.593345 | 4.4:-42711175 | None:intergenic |
| ACTGATACAAATAATTTGTG+TGG | 0.607437 | 4.4:-42711178 | None:intergenic |
| CCAAATGAAGAAGAAGTCGA+TGG | 0.658231 | 4.4:+42710739 | MS.gene61120:CDS |
| ATGGCGACCGATGAAATGTG+CGG | 0.726557 | 4.4:+42710345 | MS.gene61120:CDS |
| GCGACCGATGAAATGTGCGG+CGG | 0.753822 | 4.4:+42710348 | MS.gene61120:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CAATTTCAAAATTTTCAAAA+CGG | + | chr4.4:42710775-42710794 | MS.gene61120:CDS | 15.0% |
| !!! | GTTGGTTTTTATTTTATTTT+TGG | + | chr4.4:42710859-42710878 | MS.gene61120:intron | 15.0% |
| !!! | GTTTTTTATTTTATTTTTGC+TGG | + | chr4.4:42710881-42710900 | MS.gene61120:intron | 15.0% |
| !!! | TGTTGTTTTTTTTAATCATC+TGG | + | chr4.4:42710638-42710657 | MS.gene61120:intron | 20.0% |
| ! | CTTAAAATAGCCTTCTAAAT+GGG | - | chr4.4:42710519-42710538 | None:intergenic | 25.0% |
| !! | ACTGATACAAATAATTTGTG+TGG | - | chr4.4:42711181-42711200 | None:intergenic | 25.0% |
| !! | ATCATCAACAATTCGAATTT+TGG | - | chr4.4:42711017-42711036 | None:intergenic | 25.0% |
| !! | TTTGAAATTGTGCAAATACT+TGG | - | chr4.4:42710765-42710784 | None:intergenic | 25.0% |
| !!! | CATTTAGAAGGCTATTTTAA+GGG | + | chr4.4:42710518-42710537 | MS.gene61120:intron | 25.0% |
| AAGTTCGTGATAAAGGAAAT+TGG | + | chr4.4:42711062-42711081 | MS.gene61120:CDS | 30.0% | |
| CCTTAAAATAGCCTTCTAAA+TGG | - | chr4.4:42710520-42710539 | None:intergenic | 30.0% | |
| ! | AAAACGGTGATCATACTTTT+TGG | + | chr4.4:42710791-42710810 | MS.gene61120:CDS | 30.0% |
| ! | GATACAAATAATTTGTGTGG+AGG | - | chr4.4:42711178-42711197 | None:intergenic | 30.0% |
| !! | CCATTTAGAAGGCTATTTTA+AGG | + | chr4.4:42710517-42710536 | MS.gene61120:intron | 30.0% |
| AATTGGATGGAGAAATACGT+TGG | + | chr4.4:42711079-42711098 | MS.gene61120:CDS | 35.0% | |
| ACGAAGAAGAAGATGAAGAT+CGG | + | chr4.4:42710405-42710424 | MS.gene61120:CDS | 35.0% | |
| ACGTCTGAGAAGAAAATTCT+AGG | + | chr4.4:42710565-42710584 | MS.gene61120:intron | 35.0% | |
| CAATCTTATTGCCGTCAAAA+CGG | - | chr4.4:42710454-42710473 | None:intergenic | 35.0% | |
| CGTCTGAGAAGAAAATTCTA+GGG | + | chr4.4:42710566-42710585 | MS.gene61120:intron | 35.0% | |
| GAATGTGAAGTTCGTGATAA+AGG | + | chr4.4:42711055-42711074 | MS.gene61120:CDS | 35.0% | |
| TCGTGATAAAGGAAATTGGA+TGG | + | chr4.4:42711066-42711085 | MS.gene61120:CDS | 35.0% | |
| TGAAAATGTCTTCTTCTAGC+AGG | + | chr4.4:42710597-42710616 | MS.gene61120:intron | 35.0% | |
| TTAAGGGTTGTTGTGAATCT+TGG | + | chr4.4:42710534-42710553 | MS.gene61120:intron | 35.0% | |
| ! | TCATTTGGATTTTCGTCTAC+AGG | - | chr4.4:42710727-42710746 | None:intergenic | 35.0% |
| !! | CGAATTGTTGATGATGAAAC+TGG | + | chr4.4:42711022-42711041 | MS.gene61120:CDS | 35.0% |
| !! | GCATAGTATTAGTTCTGCTA+CGG | + | chr4.4:42710816-42710835 | MS.gene61120:CDS | 35.0% |
| !! | GTTTTGACGGCAATAAGATT+GGG | + | chr4.4:42710453-42710472 | MS.gene61120:CDS | 35.0% |
| !! | TGGATGGTTTGAGTTTGTTA+GGG | + | chr4.4:42711105-42711124 | MS.gene61120:CDS | 35.0% |
| !!! | TGGGCTTTTTAGTTATTGAC+CGG | + | chr4.4:42710472-42710491 | MS.gene61120:CDS | 35.0% |
| AGAAATACGTTGGAAGTGGA+TGG | + | chr4.4:42711089-42711108 | MS.gene61120:CDS | 40.0% | |
| AGGGCAAAAGAACTCAAAGA+AGG | + | chr4.4:42711124-42711143 | MS.gene61120:CDS | 40.0% | |
| ATGGAGAAATACGTTGGAAG+TGG | + | chr4.4:42711085-42711104 | MS.gene61120:CDS | 40.0% | |
| ATTGACCGGGCAAAAATGAA+TGG | + | chr4.4:42710486-42710505 | MS.gene61120:CDS | 40.0% | |
| CCAAATGAAGAAGAAGTCGA+TGG | + | chr4.4:42710739-42710758 | MS.gene61120:CDS | 40.0% | |
| CGAAGAAGAAGATGAAGATC+GGG | + | chr4.4:42710406-42710425 | MS.gene61120:CDS | 40.0% | |
| CTTCTTCGTCCATCTCAAAA+CGG | - | chr4.4:42710394-42710413 | None:intergenic | 40.0% | |
| CTTTGAATTGCAATCTTCCG+AGG | - | chr4.4:42710977-42710996 | None:intergenic | 40.0% | |
| GAAGATCGGGTCATTTGATT+GGG | + | chr4.4:42710419-42710438 | MS.gene61120:CDS | 40.0% | |
| TGAAGATCGGGTCATTTGAT+TGG | + | chr4.4:42710418-42710437 | MS.gene61120:CDS | 40.0% | |
| TGAATGTGTAGCGTATTCCT+CGG | + | chr4.4:42710957-42710976 | MS.gene61120:intron | 40.0% | |
| TTATTGCCGTCAAAACGGTT+CGG | - | chr4.4:42710449-42710468 | None:intergenic | 40.0% | |
| TTGACCGGGCAAAAATGAAT+GGG | + | chr4.4:42710487-42710506 | MS.gene61120:CDS | 40.0% | |
| ! | GTGGATGGTTTGAGTTTGTT+AGG | + | chr4.4:42711104-42711123 | MS.gene61120:CDS | 40.0% |
| ! | TTGCTGGAGTATTTGCACAT+GGG | + | chr4.4:42710897-42710916 | MS.gene61120:intron | 40.0% |
| ! | TTTGCTGGAGTATTTGCACA+TGG | + | chr4.4:42710896-42710915 | MS.gene61120:intron | 40.0% |
| !! | CCATCGACTTCTTCTTCATT+TGG | - | chr4.4:42710742-42710761 | None:intergenic | 40.0% |
| !! | CGTTTTGACGGCAATAAGAT+TGG | + | chr4.4:42710452-42710471 | MS.gene61120:CDS | 40.0% |
| !!! | GGGCTTTTTAGTTATTGACC+GGG | + | chr4.4:42710473-42710492 | MS.gene61120:CDS | 40.0% |
| CGATGTAATTGATCCGGAGT+TGG | + | chr4.4:42710690-42710709 | MS.gene61120:CDS | 45.0% | |
| CTGCAGCGATGTAATTGATC+CGG | + | chr4.4:42710684-42710703 | MS.gene61120:intron | 45.0% | |
| GGGCAAAAATGAATGGGTCA+CGG | + | chr4.4:42710493-42710512 | MS.gene61120:intron | 45.0% | |
| GGTATCAATTCGTCCAACTC+CGG | - | chr4.4:42710706-42710725 | None:intergenic | 45.0% | |
| ! | GGTTTTCCGAACCGTTTTGA+CGG | + | chr4.4:42710440-42710459 | MS.gene61120:CDS | 45.0% |
| !! | GTGACCCATTCATTTTTGCC+CGG | - | chr4.4:42710494-42710513 | None:intergenic | 45.0% |
| ATGGCGACCGATGAAATGTG+CGG | + | chr4.4:42710345-42710364 | MS.gene61120:CDS | 50.0% | |
| GAGTATTTGCACATGGGTGG+TGG | + | chr4.4:42710903-42710922 | MS.gene61120:intron | 50.0% | |
| ! | CTGGAGTATTTGCACATGGG+TGG | + | chr4.4:42710900-42710919 | MS.gene61120:intron | 50.0% |
| !! | ATTAGTTCTGCTACGGCTGC+TGG | + | chr4.4:42710823-42710842 | MS.gene61120:CDS | 50.0% |
| ATGAAATGTGCGGCGGATGC+AGG | + | chr4.4:42710355-42710374 | MS.gene61120:CDS | 55.0% | |
| CCATCTCAAAACGGCGACGA+TGG | - | chr4.4:42710385-42710404 | None:intergenic | 55.0% | |
| GCATCCGCCGCACATTTCAT+CGG | - | chr4.4:42710355-42710374 | None:intergenic | 55.0% | |
| TGGGTCACGGCCCATTTAGA+AGG | + | chr4.4:42710506-42710525 | MS.gene61120:intron | 55.0% | |
| ! | GCTGGACGAAGAGTTCTGGT+TGG | + | chr4.4:42710841-42710860 | MS.gene61120:intron | 55.0% |
| !!! | CCATCGTCGCCGTTTTGAGA+TGG | + | chr4.4:42710382-42710401 | MS.gene61120:CDS | 55.0% |
| GCGACCGATGAAATGTGCGG+CGG | + | chr4.4:42710348-42710367 | MS.gene61120:CDS | 60.0% | |
| GGCTGCTGGACGAAGAGTTC+TGG | + | chr4.4:42710837-42710856 | MS.gene61120:CDS | 60.0% | |
| ! | GCACATGGGTGGTGGCTTTC+TGG | + | chr4.4:42710911-42710930 | MS.gene61120:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 42710345 | 42711210 | 42710345 | ID=MS.gene61120 |
| chr4.4 | mRNA | 42710345 | 42711210 | 42710345 | ID=MS.gene61120.t1;Parent=MS.gene61120 |
| chr4.4 | exon | 42710345 | 42710508 | 42710345 | ID=MS.gene61120.t1.exon1;Parent=MS.gene61120.t1 |
| chr4.4 | CDS | 42710345 | 42710508 | 42710345 | ID=cds.MS.gene61120.t1;Parent=MS.gene61120.t1 |
| chr4.4 | exon | 42710690 | 42710858 | 42710690 | ID=MS.gene61120.t1.exon2;Parent=MS.gene61120.t1 |
| chr4.4 | CDS | 42710690 | 42710858 | 42710690 | ID=cds.MS.gene61120.t1;Parent=MS.gene61120.t1 |
| chr4.4 | exon | 42710968 | 42711210 | 42710968 | ID=MS.gene61120.t1.exon3;Parent=MS.gene61120.t1 |
| chr4.4 | CDS | 42710968 | 42711210 | 42710968 | ID=cds.MS.gene61120.t1;Parent=MS.gene61120.t1 |
| Gene Sequence |
| Protein sequence |
