AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene61258

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61258.t1 MTR_4g099240 83.439 157 23 1 126 279 181 337 1.28e-91 275
MS.gene61258.t1 MTR_4g099240 79.200 125 19 2 1 125 1 118 1.28e-59 193
MS.gene61258.t1 MTR_4g099220 88.652 141 12 2 126 264 187 325 9.96e-83 252
MS.gene61258.t1 MTR_4g099220 80.690 145 25 2 1 145 1 142 4.27e-74 229
MS.gene61258.t1 MTR_4g086855 60.667 150 59 0 126 275 104 253 2.15e-59 190
MS.gene61258.t1 MTR_4g086855 78.571 42 8 1 84 125 1 41 1.91e-14 72.0
MS.gene61258.t1 MTR_4g086855 60.667 150 59 0 126 275 199 348 2.85e-58 190
MS.gene61258.t1 MTR_4g086855 76.699 103 23 1 23 125 35 136 1.39e-49 167
MS.gene61258.t1 MTR_5g013550 60.625 160 51 4 126 279 182 335 3.54e-56 184
MS.gene61258.t1 MTR_5g013550 70.161 124 30 2 2 125 3 119 9.01e-51 170
MS.gene61258.t1 MTR_2g006330 60.274 146 51 3 126 268 104 245 3.02e-55 179
MS.gene61258.t1 MTR_2g006330 80.952 42 7 1 84 125 1 41 1.70e-14 72.0
MS.gene61258.t1 MTR_2g006330 60.274 146 51 3 126 268 180 321 1.30e-54 180
MS.gene61258.t1 MTR_2g006330 75.728 103 24 1 23 125 16 117 1.05e-47 162
MS.gene61258.t1 MTR_8g089975 63.846 130 46 1 126 255 104 232 2.19e-53 174
MS.gene61258.t1 MTR_8g089975 83.333 42 6 1 84 125 1 41 3.06e-15 73.9
MS.gene61258.t1 MTR_8g089975 63.846 130 46 1 126 255 180 308 1.86e-52 174
MS.gene61258.t1 MTR_8g089975 76.699 103 23 1 23 125 16 117 1.44e-48 164
MS.gene61258.t1 MTR_1g034030 62.698 126 47 0 126 251 200 325 2.78e-52 174
MS.gene61258.t1 MTR_1g034030 75.728 103 24 1 23 125 36 137 1.92e-49 167
MS.gene61258.t1 MTR_5g064540 55.844 154 63 2 126 279 180 328 5.79e-52 173
MS.gene61258.t1 MTR_5g064540 74.510 102 25 1 25 126 18 118 1.19e-47 162
MS.gene61258.t1 MTR_8g095330 61.940 134 50 1 126 258 181 314 1.57e-51 172
MS.gene61258.t1 MTR_8g095330 74.757 103 25 1 23 125 17 118 2.37e-47 161
MS.gene61258.t1 MTR_8g079560 55.479 146 63 1 126 271 199 342 2.49e-51 172
MS.gene61258.t1 MTR_8g079560 74.757 103 25 1 23 125 35 136 2.11e-48 164
MS.gene61258.t1 MTR_3g097320 59.559 136 50 2 126 258 180 313 6.64e-51 171
MS.gene61258.t1 MTR_3g097320 71.845 103 28 1 23 125 16 117 1.16e-45 157
MS.gene61258.t1 MTR_5g018050 61.111 126 49 0 126 251 161 286 8.92e-51 169
MS.gene61258.t1 MTR_5g018050 74.684 79 19 1 47 125 21 98 3.38e-36 131
MS.gene61258.t1 MTR_5g018050 61.111 126 49 0 126 251 194 319 2.06e-50 169
MS.gene61258.t1 MTR_5g018050 74.757 103 25 1 23 125 30 131 7.08e-49 166
MS.gene61258.t1 MTR_5g018050 74.757 103 25 1 23 125 30 131 8.04e-50 165
MS.gene61258.t1 MTR_5g018050 79.412 34 7 0 126 159 194 227 7.22e-14 70.1
MS.gene61258.t1 MTR_1g034030 75.728 103 24 1 23 125 36 137 1.82e-49 166
MS.gene61258.t1 MTR_1g034030 80.882 68 13 0 126 193 200 267 2.71e-32 121
MS.gene61258.t1 MTR_5g064540 74.510 102 25 1 25 126 18 118 9.62e-49 162
MS.gene61258.t1 MTR_5g064540 72.059 68 19 0 126 193 180 247 1.98e-29 112
MS.gene61258.t1 MTR_3g086940 60.769 130 48 1 126 252 181 310 3.15e-48 164
MS.gene61258.t1 MTR_3g086940 64.800 125 37 2 1 125 1 118 1.55e-46 159
MS.gene61258.t1 MTR_8g088740 58.416 101 41 1 25 125 18 117 4.95e-37 130
MS.gene61258.t1 MTR_6g048250 33.597 253 97 7 23 211 31 276 1.10e-32 123
MS.gene61258.t1 MTR_6g012990 33.597 253 97 7 23 211 31 276 4.76e-32 121
MS.gene61258.t1 MTR_6g048250 33.597 253 97 7 23 211 31 276 5.42e-32 122
MS.gene61258.t1 MTR_6g048250 33.597 253 97 7 23 211 31 276 9.37e-32 123
MS.gene61258.t1 MTR_6g012990 33.597 253 97 7 23 211 31 276 2.21e-31 122
MS.gene61258.t1 MTR_3g099920 32.411 253 100 7 23 211 31 276 2.87e-30 119
MS.gene61258.t1 MTR_8g088745 46.087 115 60 2 139 251 1 115 5.13e-30 110
MS.gene61258.t1 MTR_4g114670 30.932 236 104 5 26 209 26 254 1.98e-29 115
MS.gene61258.t1 MTR_5g088350 27.907 258 112 7 26 216 28 278 1.74e-23 98.2
MS.gene61258.t1 MTR_5g088350 27.907 258 112 7 26 216 28 278 3.37e-23 98.6
MS.gene61258.t1 MTR_5g088350 27.907 258 112 7 26 216 28 278 8.56e-23 97.4
MS.gene61258.t1 MTR_4g114670 27.711 249 108 7 26 209 26 267 8.86e-23 96.7
MS.gene61258.t1 MTR_4g114670 27.711 249 108 7 26 209 26 267 1.60e-22 96.7
MS.gene61258.t1 MTR_2g045470 41.525 118 62 3 13 124 20 136 1.43e-21 94.0
MS.gene61258.t1 MTR_8g024600 29.839 248 102 8 26 208 32 272 1.46e-21 94.0
MS.gene61258.t1 MTR_5g067150 28.185 259 113 8 26 218 24 275 6.75e-21 91.3
MS.gene61258.t1 MTR_5g067150 28.185 259 113 8 26 218 24 275 8.54e-21 91.7
MS.gene61258.t1 MTR_5g067150 28.185 259 113 8 26 218 24 275 9.80e-21 91.7
MS.gene61258.t1 MTR_8g024590 29.032 248 104 8 26 208 32 272 1.04e-19 88.6
MS.gene61258.t1 MTR_5g075100 47.059 102 47 3 28 124 40 139 6.70e-19 86.3
MS.gene61258.t1 MTR_4g131060 27.559 254 109 8 28 214 35 280 6.63e-18 83.2
MS.gene61258.t1 MTR_4g123940 27.092 251 109 7 23 206 38 281 8.02e-18 82.4
MS.gene61258.t1 MTR_4g123940 27.092 251 109 7 23 206 38 281 1.10e-17 82.8
MS.gene61258.t1 MTR_6g012980 42.727 110 57 3 23 127 85 193 1.28e-17 82.4
MS.gene61258.t1 MTR_6g012980 39.394 99 53 3 114 212 240 331 1.87e-14 73.2
MS.gene61258.t1 MTR_2g049790 26.374 273 125 8 13 216 23 288 1.91e-17 82.0
MS.gene61258.t1 MTR_4g029020 26.829 246 105 9 37 215 38 275 5.23e-17 80.9
MS.gene61258.t1 MTR_3g466480 28.226 248 111 7 28 212 38 281 6.48e-17 80.5
MS.gene61258.t1 MTR_2g016340 26.587 252 110 8 28 212 29 272 1.18e-16 79.7
MS.gene61258.t1 MTR_4g006970 28.455 246 101 9 28 206 37 274 1.46e-16 79.3
MS.gene61258.t1 MTR_8g086380 27.530 247 105 8 27 206 42 281 2.42e-16 79.0
MS.gene61258.t1 MTR_4g128820 26.939 245 108 7 26 206 25 262 4.78e-16 78.2
MS.gene61258.t1 MTR_3g072320 28.750 240 94 8 28 200 29 258 6.67e-16 77.4
MS.gene61258.t1 MTR_1g013700 44.554 101 50 3 28 124 30 128 1.28e-15 76.6
MS.gene61258.t1 MTR_1g013700 41.667 84 43 2 127 210 194 271 7.42e-13 68.6
MS.gene61258.t1 MTR_1g076370 43.478 92 46 3 37 123 47 137 1.76e-15 76.3
MS.gene61258.t1 MTR_7g072575 27.451 255 110 10 28 215 29 275 3.32e-15 75.5
MS.gene61258.t1 MTR_7g072605 41.176 102 54 2 28 124 33 133 5.63e-14 71.6
MS.gene61258.t1 MTR_0464s0040 34.188 117 69 4 13 123 12 126 1.21e-13 68.6
MS.gene61258.t1 MTR_5g075060 26.693 251 109 8 23 206 24 266 1.47e-13 70.9
MS.gene61258.t1 MTR_1g103760 43.564 101 51 4 28 124 29 127 1.63e-13 70.5
MS.gene61258.t1 MTR_3g466400 39.252 107 57 4 23 124 26 129 3.43e-12 66.6
MS.gene61258.t1 MTR_2g105010 29.348 184 112 7 25 206 101 268 3.57e-12 66.6
MS.gene61258.t1 MTR_1g040875 38.947 95 52 3 34 123 47 140 5.01e-12 65.9
MS.gene61258.t1 MTR_1g101630 34.375 128 72 4 1 123 37 157 1.55e-11 64.7
MS.gene61258.t1 MTR_8g024600 32.237 152 88 7 57 208 53 189 2.03e-11 63.9
MS.gene61258.t1 MTR_2g019575 41.463 82 42 3 127 208 80 155 2.34e-11 63.2
MS.gene61258.t1 MTR_2g019575 41.463 82 42 3 127 208 80 155 2.70e-11 63.5
MS.gene61258.t1 MTR_8g040300 34.483 87 53 1 123 209 182 264 9.63e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61258.t1 AT1G60940 51.475 305 76 6 23 258 16 317 7.70e-96 286
MS.gene61258.t1 AT1G60940 51.475 305 76 6 23 258 16 317 7.70e-96 286
MS.gene61258.t1 AT4G33950 61.268 142 55 0 126 267 197 338 1.37e-56 186
MS.gene61258.t1 AT4G33950 74.757 103 25 1 23 125 33 134 1.07e-48 165
MS.gene61258.t1 AT4G33950 60.284 141 54 1 126 264 197 337 7.71e-55 181
MS.gene61258.t1 AT4G33950 74.757 103 25 1 23 125 33 134 7.86e-49 165
MS.gene61258.t1 AT5G08590 60.150 133 53 0 127 259 105 237 2.41e-54 177
MS.gene61258.t1 AT5G08590 71.429 42 11 1 84 125 1 41 6.18e-12 65.1
MS.gene61258.t1 AT5G08590 60.150 133 53 0 127 259 181 313 1.33e-53 178
MS.gene61258.t1 AT5G08590 73.786 103 26 1 23 125 16 117 1.39e-46 159
MS.gene61258.t1 AT5G63650 63.158 133 44 2 127 256 181 311 2.78e-52 174
MS.gene61258.t1 AT5G63650 71.845 103 28 1 23 125 16 117 4.90e-45 155
MS.gene61258.t1 AT3G50500 60.000 130 52 0 126 255 199 328 1.83e-51 172
MS.gene61258.t1 AT3G50500 76.699 103 23 1 23 125 35 136 7.30e-49 166
MS.gene61258.t1 AT3G50500 60.000 130 52 0 126 255 206 335 1.91e-51 172
MS.gene61258.t1 AT3G50500 76.699 103 23 1 23 125 35 136 8.24e-49 166
MS.gene61258.t1 AT5G66880 60.000 130 52 0 126 255 198 327 7.70e-50 168
MS.gene61258.t1 AT5G66880 74.757 103 25 1 23 125 34 135 1.98e-48 164
MS.gene61258.t1 AT5G66880 60.000 130 52 0 126 255 198 327 7.70e-50 168
MS.gene61258.t1 AT5G66880 74.757 103 25 1 23 125 34 135 1.98e-48 164
MS.gene61258.t1 AT1G10940 63.200 125 41 2 126 247 180 302 1.22e-49 168
MS.gene61258.t1 AT1G10940 75.728 103 24 1 23 125 16 117 4.86e-48 164
MS.gene61258.t1 AT1G10940 63.200 125 41 2 126 247 188 310 1.23e-49 168
MS.gene61258.t1 AT1G10940 70.270 111 24 2 23 125 16 125 4.05e-45 156
MS.gene61258.t1 AT1G78290 63.934 122 39 1 126 247 180 296 2.51e-46 159
MS.gene61258.t1 AT1G78290 71.845 103 28 1 23 125 16 117 5.14e-45 155
MS.gene61258.t1 AT1G78290 63.934 122 39 1 126 247 180 296 2.51e-46 159
MS.gene61258.t1 AT1G78290 71.845 103 28 1 23 125 16 117 5.14e-45 155
MS.gene61258.t1 AT4G40010 59.420 138 50 2 126 258 180 316 1.71e-45 157
MS.gene61258.t1 AT4G40010 70.874 103 29 1 23 125 16 117 1.62e-44 154
MS.gene61258.t1 AT2G23030 51.316 152 69 2 128 275 182 332 3.23e-45 155
MS.gene61258.t1 AT2G23030 66.019 103 34 1 23 125 16 117 5.85e-41 144
MS.gene61258.t1 AT3G01090 35.178 253 93 6 23 211 31 276 3.47e-35 132
MS.gene61258.t1 AT3G01090 35.178 253 93 6 23 211 31 276 3.47e-35 132
MS.gene61258.t1 AT3G01090 35.178 253 93 6 23 211 54 299 5.22e-35 132
MS.gene61258.t1 AT3G29160 34.783 253 94 7 23 211 32 277 2.22e-32 122
MS.gene61258.t1 AT3G29160 34.783 253 94 7 23 211 32 277 2.03e-31 122
MS.gene61258.t1 AT3G29160 34.783 253 94 7 23 211 32 277 2.03e-31 122
MS.gene61258.t1 AT4G11373 63.636 88 24 2 39 126 1 80 5.34e-31 112
MS.gene61258.t1 AT5G39440 32.927 246 103 7 25 211 33 275 1.01e-24 103
MS.gene61258.t1 AT2G26980 28.295 258 111 7 26 216 29 279 4.47e-24 100
MS.gene61258.t1 AT4G24400 28.736 261 113 8 25 219 23 276 6.12e-24 100
MS.gene61258.t1 AT2G26980 28.295 258 111 7 26 216 29 279 8.58e-24 100
MS.gene61258.t1 AT4G24400 28.846 260 112 8 26 219 24 276 9.64e-24 100
MS.gene61258.t1 AT2G26980 28.295 258 111 7 26 216 29 279 1.05e-23 99.8
MS.gene61258.t1 AT2G26980 28.295 258 111 7 26 216 39 289 1.08e-23 100
MS.gene61258.t1 AT5G35410 28.916 249 105 7 26 209 26 267 1.97e-22 95.9
MS.gene61258.t1 AT5G35410 28.916 249 105 7 26 209 26 267 3.61e-22 95.9
MS.gene61258.t1 AT1G01140 26.316 304 132 9 13 231 19 315 5.83e-21 92.4
MS.gene61258.t1 AT1G01140 26.316 304 132 9 13 231 19 315 6.36e-21 92.0
MS.gene61258.t1 AT1G01140 25.987 304 135 9 13 231 19 317 1.61e-20 90.9
MS.gene61258.t1 AT2G26980 43.689 103 53 2 26 124 29 130 2.27e-20 88.6
MS.gene61258.t1 AT1G30270 27.170 265 117 8 13 208 31 288 2.33e-20 90.9
MS.gene61258.t1 AT1G30270 27.170 265 117 8 13 208 31 288 2.39e-20 90.5
MS.gene61258.t1 AT5G10930 28.980 245 98 8 34 210 35 271 3.72e-20 90.1
MS.gene61258.t1 AT1G48260 42.373 118 61 3 13 124 11 127 8.77e-19 85.9
MS.gene61258.t1 AT2G25880 26.389 216 125 5 23 209 37 247 2.45e-18 82.8
MS.gene61258.t1 AT5G21326 43.689 103 53 2 26 124 28 129 2.72e-18 84.7
MS.gene61258.t1 AT5G21326 34.167 120 65 3 127 246 195 300 2.46e-13 70.1
MS.gene61258.t1 AT3G17510 38.983 118 65 3 13 124 20 136 6.59e-18 83.6
MS.gene61258.t1 AT5G21222 41.509 106 57 2 23 124 25 129 1.19e-17 83.2
MS.gene61258.t1 AT5G21222 34.167 120 65 3 127 246 195 300 1.07e-12 68.6
MS.gene61258.t1 AT5G21222 41.509 106 57 2 23 124 25 129 1.19e-17 83.2
MS.gene61258.t1 AT5G21222 34.167 120 65 3 127 246 195 300 1.07e-12 68.6
MS.gene61258.t1 AT5G21222 41.509 106 57 2 23 124 25 129 1.19e-17 83.2
MS.gene61258.t1 AT5G21222 34.167 120 65 3 127 246 195 300 1.07e-12 68.6
MS.gene61258.t1 AT5G21222 41.509 106 57 2 23 124 25 129 1.19e-17 83.2
MS.gene61258.t1 AT5G21222 34.167 120 65 3 127 246 195 300 1.07e-12 68.6
MS.gene61258.t1 AT2G30360 41.176 102 54 2 28 124 38 138 2.26e-17 82.0
MS.gene61258.t1 AT4G14580 42.157 102 53 2 27 123 37 137 5.94e-17 80.5
MS.gene61258.t1 AT5G25110 38.636 132 71 5 1 124 29 158 2.34e-16 79.0
MS.gene61258.t1 AT5G25110 39.773 88 47 2 127 214 224 305 4.00e-14 72.4
MS.gene61258.t1 AT4G18700 26.693 251 109 8 27 210 42 284 2.48e-16 79.0
MS.gene61258.t1 AT5G01820 41.584 101 53 3 28 124 39 137 3.41e-16 78.6
MS.gene61258.t1 AT5G45810 27.092 251 108 8 27 210 44 286 5.20e-16 78.2
MS.gene61258.t1 AT5G01810 26.000 250 110 8 28 210 29 270 3.62e-15 75.5
MS.gene61258.t1 AT5G01810 26.000 250 110 8 28 210 29 270 3.62e-15 75.5
MS.gene61258.t1 AT5G01810 26.000 250 110 8 28 210 29 270 5.17e-15 74.7
MS.gene61258.t1 AT2G25090 40.816 98 52 2 32 124 36 132 5.37e-15 75.1
MS.gene61258.t1 AT2G25090 38.824 85 46 2 126 210 204 282 3.32e-12 66.6
MS.gene61258.t1 AT5G07070 26.693 251 109 9 27 210 28 270 7.00e-15 74.7
MS.gene61258.t1 AT5G45820 25.352 284 127 10 28 244 29 294 7.40e-15 74.3
MS.gene61258.t1 AT2G38490 42.268 97 51 3 31 124 49 143 2.83e-14 72.8
MS.gene61258.t1 AT5G58380 25.714 245 107 9 33 210 34 270 2.54e-13 70.1
MS.gene61258.t1 AT2G45490 37.931 87 50 1 123 209 194 276 2.64e-13 68.9
MS.gene61258.t1 AT5G57630 37.864 103 58 3 26 124 27 127 2.92e-13 69.7
MS.gene61258.t1 AT2G34180 40.196 102 55 3 27 124 73 172 2.95e-13 69.7
MS.gene61258.t1 AT4G30960 26.126 222 93 7 50 208 67 280 2.95e-13 69.7
MS.gene61258.t1 AT5G57630 37.864 103 58 3 26 124 27 127 3.30e-13 69.7
MS.gene61258.t1 AT1G29230 39.216 102 56 3 27 124 90 189 7.12e-13 68.9
MS.gene61258.t1 AT3G23000 39.216 102 56 3 27 123 41 141 7.94e-13 68.6
MS.gene61258.t1 AT1G12580 32.031 128 75 3 1 123 40 160 7.07e-11 62.8

Find 69 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAAATTTCTAGGATCTTC+TGG 0.188809 8.2:+22946906 None:intergenic
GCAGGACCAGGTTCCTTTAA+TGG 0.222418 8.2:+22948756 None:intergenic
TCTTTGAATCTGATTATATT+AGG 0.222870 8.2:+22948391 None:intergenic
ATAGTCTTTCTGAAATTTCT+AGG 0.223677 8.2:+22946897 None:intergenic
GAAATTTCAGAAAGACTATT+GGG 0.306280 8.2:-22946893 MS.gene61258:CDS
TTATTCCAACAGCTAATATC+TGG 0.312504 8.2:-22947584 MS.gene61258:CDS
TCTGGTTGGTAGAGAATAAT+AGG 0.320796 8.2:-22946791 MS.gene61258:intron
TCAGCTTCAATTATCTCTTC+AGG 0.323000 8.2:+22946366 None:intergenic
AGAAATTTCAGAAAGACTAT+TGG 0.328126 8.2:-22946894 MS.gene61258:CDS
ATGACGAGGAAATTGATATC+AGG 0.347137 8.2:-22946200 MS.gene61258:CDS
GTACATAGAGAGGGGTAAAA+AGG 0.349105 8.2:-22948688 MS.gene61258:intron
TTGGAGTATGCGGCGGCTGC+TGG 0.386153 8.2:-22947770 MS.gene61258:CDS
GGATCTTCTGGTTCTTCAAA+TGG 0.399417 8.2:+22946918 None:intergenic
ACACTTTATGTTATGCTAGT+TGG 0.432447 8.2:-22946948 MS.gene61258:CDS
CGTCTACACTTTGGATTGGT+TGG 0.438514 8.2:+22946313 None:intergenic
TCGAAAGCTGGCGATGGTGC+TGG 0.441541 8.2:-22946246 MS.gene61258:CDS
TTCTCATCTAGTCATTGTCT+TGG 0.446749 8.2:-22947789 MS.gene61258:CDS
GACTGAAATATGTACATTGT+CGG 0.461133 8.2:+22946751 None:intergenic
CAACAAAGATGCGAGATAGA+AGG 0.472933 8.2:+22946717 None:intergenic
TCATGATTTCGTCTACACTT+TGG 0.474075 8.2:+22946304 None:intergenic
AATATGTACATTGTCGGGTA+TGG 0.481339 8.2:+22946757 None:intergenic
ATTTCGTCTACACTTTGGAT+TGG 0.483240 8.2:+22946309 None:intergenic
CAAAGATAAAACAGGACCCT+TGG 0.489134 8.2:-22946404 MS.gene61258:CDS
ACATTGGTACATACCTTGGC+AGG 0.490009 8.2:+22946689 None:intergenic
CACCATCCCAAAGATAAAAC+AGG 0.491345 8.2:-22946412 MS.gene61258:CDS
TGTGGTTAATTATGTCCCTC+TGG 0.493984 8.2:+22948428 None:intergenic
ACTGAAATATGTACATTGTC+GGG 0.498140 8.2:+22946752 None:intergenic
CCAGCTTTCGATCCTTGTCC+CGG 0.498254 8.2:+22946258 None:intergenic
TTCAAGAGGATACGCACCGC+TGG 0.498347 8.2:-22947740 MS.gene61258:CDS
AGAGCGTTATGAGCCATTAG+AGG 0.508214 8.2:-22948802 MS.gene61258:CDS
TTGTCTTTAGCCAACCTAGC+AGG 0.512029 8.2:+22948738 None:intergenic
GGAACCTGGTCCTGCTAGGT+TGG 0.541285 8.2:-22948748 MS.gene61258:CDS
ATACAAGAAGCGAGGGTACC+GGG 0.542520 8.2:-22946276 MS.gene61258:CDS
GGTACATACCTTGGCAGGAT+CGG 0.545526 8.2:+22946694 None:intergenic
TTAGCCAACCTAGCAGGACC+AGG 0.548718 8.2:+22948744 None:intergenic
CCGGGACAAGGATCGAAAGC+TGG 0.552791 8.2:-22946258 MS.gene61258:CDS
TGTTAGCTACTGTCATTCAA+TGG 0.554651 8.2:-22947561 MS.gene61258:intron
CTTTGTTGCCGATCCTGCCA+AGG 0.554698 8.2:-22946702 MS.gene61258:intron
AACATAAAGTGTCACACCAC+AGG 0.555054 8.2:+22946959 None:intergenic
CATACAAGAAGCGAGGGTAC+CGG 0.556086 8.2:-22946277 MS.gene61258:CDS
TAAAGGAACCTGGTCCTGCT+AGG 0.558021 8.2:-22948752 MS.gene61258:CDS
TGACGAGGAAATTGATATCA+GGG 0.563689 8.2:-22946199 MS.gene61258:CDS
ATGTTATGTGGAAACAAAGA+AGG 0.570996 8.2:-22946337 MS.gene61258:CDS
TTGTATAGATTGCAGATGTG+TGG 0.578652 8.2:-22946983 MS.gene61258:intron
AAGACAATGACTAGATGAGA+AGG 0.585565 8.2:+22947791 None:intergenic
CAAGGATCGAAAGCTGGCGA+TGG 0.586251 8.2:-22946252 MS.gene61258:CDS
TAGGATGCTTTAATGATCTG+TGG 0.587087 8.2:+22948410 None:intergenic
TGCTCTCAAGTACATAGAGA+GGG 0.588141 8.2:-22948697 MS.gene61258:CDS
AGACAATGACTAGATGAGAA+GGG 0.590272 8.2:+22947792 None:intergenic
GAAGCGAGGGTACCGGGACA+AGG 0.594784 8.2:-22946270 MS.gene61258:CDS
AATCAGCCATTAAAGGAACC+TGG 0.597077 8.2:-22948762 MS.gene61258:CDS
ATTGCAGATGTGTGGTCCTG+TGG 0.607065 8.2:-22946975 MS.gene61258:CDS
TGGACGATTTAGTGAAGATG+AGG 0.610578 8.2:-22947720 MS.gene61258:intron
AGCTGAAAGAAAATGTTATG+TGG 0.610913 8.2:-22946349 MS.gene61258:CDS
GACAATGACTAGATGAGAAG+GGG 0.614214 8.2:+22947793 None:intergenic
TAATATAATCAGATTCAAAG+AGG 0.615290 8.2:-22948389 MS.gene61258:intron
TACATATTTCAGTCGAGTGT+AGG 0.615992 8.2:-22946742 MS.gene61258:CDS
TAACACCAGATATTAGCTGT+TGG 0.619127 8.2:+22947579 None:intergenic
GAGGATCATACAAGAAGCGA+GGG 0.631597 8.2:-22946283 MS.gene61258:CDS
TTGCTCTCAAGTACATAGAG+AGG 0.636123 8.2:-22948698 MS.gene61258:CDS
TGAGGATCATACAAGAAGCG+AGG 0.644704 8.2:-22946284 MS.gene61258:CDS
AGTCATTGTCTTGGAGTATG+CGG 0.648873 8.2:-22947780 MS.gene61258:CDS
CATTGTCTTGGAGTATGCGG+CGG 0.650071 8.2:-22947777 MS.gene61258:CDS
GGTTGATGAGAGTGTCCAGA+GGG 0.659915 8.2:-22948443 MS.gene61258:CDS
AAATTTCAGAAAGACTATTG+GGG 0.660159 8.2:-22946892 MS.gene61258:intron
ATCTTCACTAAATCGTCCAG+CGG 0.675190 8.2:+22947724 None:intergenic
AAAGTGTAGACGAAATCATG+AGG 0.683282 8.2:-22946302 MS.gene61258:CDS
GCTCTCAAGTACATAGAGAG+GGG 0.692576 8.2:-22948696 MS.gene61258:CDS
AGGTTGATGAGAGTGTCCAG+AGG 0.743801 8.2:-22948444 MS.gene61258:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATGATATATATCCAAAA+AGG + chr8.2:22947639-22947658 None:intergenic 15.0%
!! ATTAGAATTACAAATATTCA+TGG - chr8.2:22948384-22948403 MS.gene61258:intron 15.0%
!! AAATAAAAAAGTTCCTCTAA+TGG + chr8.2:22946200-22946219 None:intergenic 20.0%
!! ATTTCTTATACTTGATAAAG+AGG - chr8.2:22948549-22948568 MS.gene61258:intron 20.0%
!! ATTTGTAATTCTAATGCTAT+AGG + chr8.2:22948379-22948398 None:intergenic 20.0%
!! GATAGAATTAATGCAAAAAA+AGG - chr8.2:22948475-22948494 MS.gene61258:intron 20.0%
!! GATATCTTATATTATATCCA+AGG - chr8.2:22947661-22947680 MS.gene61258:intron 20.0%
!! TAATATAATCAGATTCAAAG+AGG - chr8.2:22946597-22946616 MS.gene61258:intron 20.0%
!! TCTTTGAATCTGATTATATT+AGG + chr8.2:22946598-22946617 None:intergenic 20.0%
!! TTAACAAATACTATTAACCT+TGG + chr8.2:22947681-22947700 None:intergenic 20.0%
!! TTATCTTAAACTCTTCATTA+AGG - chr8.2:22946444-22946463 MS.gene61258:intron 20.0%
!!! AATTTTCCTATATTTGTTAC+AGG - chr8.2:22947371-22947390 MS.gene61258:intron 20.0%
!!! AGAGAATTTTTGGAAAAATT+TGG + chr8.2:22946976-22946995 None:intergenic 20.0%
!!! GAGAATTTTTGGAAAAATTT+GGG + chr8.2:22946975-22946994 None:intergenic 20.0%
!!! TACATTATTTTCCATTTCTT+AGG + chr8.2:22948416-22948435 None:intergenic 20.0%
! AAAATGATTGAGAAAATCTC+AGG + chr8.2:22947832-22947851 None:intergenic 25.0%
! AAATTTCAGAAAGACTATTG+GGG - chr8.2:22948094-22948113 MS.gene61258:intron 25.0%
! AAGCTCTCAATAATACAAAT+TGG + chr8.2:22947119-22947138 None:intergenic 25.0%
! AGAAATTTCAGAAAGACTAT+TGG - chr8.2:22948092-22948111 MS.gene61258:intron 25.0%
! AGCAAAATATCAATTCAATG+AGG + chr8.2:22946342-22946361 None:intergenic 25.0%
! ATTTGTCAATTGTTAAACCA+TGG + chr8.2:22947902-22947921 None:intergenic 25.0%
! GAAATTTCAGAAAGACTATT+GGG - chr8.2:22948093-22948112 MS.gene61258:intron 25.0%
!! AAGTTGAAAATTTTAAGCTG+AGG + chr8.2:22947577-22947596 None:intergenic 25.0%
!! AGTTGAAAATTTTAAGCTGA+GGG + chr8.2:22947576-22947595 None:intergenic 25.0%
!! GAAGCTAAAAAGAGAATTTT+TGG + chr8.2:22946986-22947005 None:intergenic 25.0%
!! TCACATAAAATGATCCTTTT+TGG - chr8.2:22947622-22947641 MS.gene61258:intron 25.0%
!! TCTTAATCTGTTTCTTGTTT+AGG - chr8.2:22946634-22946653 MS.gene61258:intron 25.0%
!! TGATTAAAAAGTTCACGTTT+TGG - chr8.2:22947943-22947962 MS.gene61258:intron 25.0%
!!! ATAGTCTTTCTGAAATTTCT+AGG + chr8.2:22948092-22948111 None:intergenic 25.0%
!!! TTTTTTGAATCAGCCATTAA+AGG - chr8.2:22946217-22946236 MS.gene61258:CDS 25.0%
AACATCAGTCAATGTTACAT+TGG + chr8.2:22948316-22948335 None:intergenic 30.0%
AATTCTATCAGTGATGTTAG+AGG + chr8.2:22948464-22948483 None:intergenic 30.0%
AATTGGTTATTATCGTTGAC+GGG - chr8.2:22946827-22946846 MS.gene61258:intron 30.0%
ACACTTTATGTTATGCTAGT+TGG - chr8.2:22948038-22948057 MS.gene61258:intron 30.0%
ACTGAAATATGTACATTGTC+GGG + chr8.2:22948237-22948256 None:intergenic 30.0%
AGAATGATCAAATTTCGATC+CGG + chr8.2:22947020-22947039 None:intergenic 30.0%
AGCTGAAAGAAAATGTTATG+TGG - chr8.2:22948637-22948656 MS.gene61258:intron 30.0%
ATAATGTAACTAATCATCCG+TGG - chr8.2:22948428-22948447 MS.gene61258:CDS 30.0%
ATCTTGCCTGTAACAAATAT+AGG + chr8.2:22947380-22947399 None:intergenic 30.0%
ATGTTATGTGGAAACAAAGA+AGG - chr8.2:22948649-22948668 MS.gene61258:intron 30.0%
ATTCTGCGCAAAAAGTTATA+TGG - chr8.2:22947035-22947054 MS.gene61258:intron 30.0%
CAATTGGTTATTATCGTTGA+CGG - chr8.2:22946826-22946845 MS.gene61258:intron 30.0%
GACTGAAATATGTACATTGT+CGG + chr8.2:22948238-22948257 None:intergenic 30.0%
GCAAATTCTTCAAAAACCAA+GGG + chr8.2:22948601-22948620 None:intergenic 30.0%
GCTAATAGCTAAAATACTCA+TGG + chr8.2:22946922-22946941 None:intergenic 30.0%
GGCATTATGAATGTAGTATT+TGG - chr8.2:22946665-22946684 MS.gene61258:intron 30.0%
GTGCATCATTACTAAAAGAA+AGG - chr8.2:22947062-22947081 MS.gene61258:intron 30.0%
GTTGGCTAAAGACAAAAATA+CGG - chr8.2:22946256-22946275 MS.gene61258:CDS 30.0%
TATGAATGATTTCTGATACC+AGG - chr8.2:22948152-22948171 MS.gene61258:intron 30.0%
TGTAATTCTAATGCTATAGG+TGG + chr8.2:22948376-22948395 None:intergenic 30.0%
TTAGCTTCTTAGTATAACAC+CGG - chr8.2:22946998-22947017 MS.gene61258:intron 30.0%
TTATTCCAACAGCTAATATC+TGG - chr8.2:22947402-22947421 MS.gene61258:intron 30.0%
TTGGCTAAAGACAAAAATAC+GGG - chr8.2:22946257-22946276 MS.gene61258:CDS 30.0%
! ACATGATTTTCCTTATGAGT+CGG + chr8.2:22946784-22946803 None:intergenic 30.0%
! GTTGAAAATTTTAAGCTGAG+GGG + chr8.2:22947575-22947594 None:intergenic 30.0%
! GTTTCTTGTTTAGGTTTGTA+GGG - chr8.2:22946643-22946662 MS.gene61258:intron 30.0%
! TGTATACGATTTTCGTGAAA+AGG + chr8.2:22947330-22947349 None:intergenic 30.0%
! TGTTTCTTGTTTAGGTTTGT+AGG - chr8.2:22946642-22946661 MS.gene61258:intron 30.0%
! TTTCTTGTTTAGGTTTGTAG+GGG - chr8.2:22946644-22946663 MS.gene61258:intron 30.0%
!!! ATTTTAACTCTGATTCTCTC+TGG - chr8.2:22948177-22948196 MS.gene61258:intron 30.0%
AAAGTGTAGACGAAATCATG+AGG - chr8.2:22948684-22948703 MS.gene61258:intron 35.0%
AAGACAATGACTAGATGAGA+AGG + chr8.2:22947198-22947217 None:intergenic 35.0%
AATATGTACATTGTCGGGTA+TGG + chr8.2:22948232-22948251 None:intergenic 35.0%
AATATTGCACACTCAACCAA+AGG - chr8.2:22947738-22947757 MS.gene61258:CDS 35.0%
AATTGGAAAACACTCTTGGA+TGG - chr8.2:22947541-22947560 MS.gene61258:intron 35.0%
AGACAATGACTAGATGAGAA+GGG + chr8.2:22947197-22947216 None:intergenic 35.0%
ATAATAACCAATTGCGCCAA+GGG + chr8.2:22946820-22946839 None:intergenic 35.0%
ATGACGAGGAAATTGATATC+AGG - chr8.2:22948786-22948805 MS.gene61258:CDS 35.0%
ATGTGAAATCCTGTTAACCT+TGG + chr8.2:22947608-22947627 None:intergenic 35.0%
ATTTCGTCTACACTTTGGAT+TGG + chr8.2:22948680-22948699 None:intergenic 35.0%
CATGGTAATTGCCTAAGAAA+TGG - chr8.2:22948402-22948421 MS.gene61258:CDS 35.0%
CTGAAATTGGAAAACACTCT+TGG - chr8.2:22947537-22947556 MS.gene61258:intron 35.0%
CTGTAGGAAATTTGTCATAC+AGG - chr8.2:22947513-22947532 MS.gene61258:intron 35.0%
GAATCAGAGTTAAAATTGCC+TGG + chr8.2:22948173-22948192 None:intergenic 35.0%
GGCAAATTCTTCAAAAACCA+AGG + chr8.2:22948602-22948621 None:intergenic 35.0%
TAACACCAGATATTAGCTGT+TGG + chr8.2:22947410-22947429 None:intergenic 35.0%
TACATATTTCAGTCGAGTGT+AGG - chr8.2:22948244-22948263 MS.gene61258:intron 35.0%
TACATTATGCTTTGTGCTGT+AGG - chr8.2:22947497-22947516 MS.gene61258:intron 35.0%
TAGGATGCTTTAATGATCTG+TGG + chr8.2:22946579-22946598 None:intergenic 35.0%
TCAGCTTCAATTATCTCTTC+AGG + chr8.2:22948623-22948642 None:intergenic 35.0%
TCATGATTTCGTCTACACTT+TGG + chr8.2:22948685-22948704 None:intergenic 35.0%
TGACGAGGAAATTGATATCA+GGG - chr8.2:22948787-22948806 MS.gene61258:CDS 35.0%
TGTTACATTGGTACATACCT+TGG + chr8.2:22948304-22948323 None:intergenic 35.0%
TGTTAGCTACTGTCATTCAA+TGG - chr8.2:22947425-22947444 MS.gene61258:intron 35.0%
TGTTATGAGCCATAACAAGT+GGG + chr8.2:22947878-22947897 None:intergenic 35.0%
TTCTCATCTAGTCATTGTCT+TGG - chr8.2:22947197-22947216 MS.gene61258:intron 35.0%
TTGTATAGATTGCAGATGTG+TGG - chr8.2:22948003-22948022 MS.gene61258:intron 35.0%
TTGTTATGAGCCATAACAAG+TGG + chr8.2:22947879-22947898 None:intergenic 35.0%
TTTCAACTTCGATTACTCCA+AGG - chr8.2:22947588-22947607 MS.gene61258:CDS 35.0%
! AAAATTTTAAGCTGAGGGGA+TGG + chr8.2:22947571-22947590 None:intergenic 35.0%
! AAGGGTCCTGTTTTATCTTT+GGG + chr8.2:22948583-22948602 None:intergenic 35.0%
! GTCGATTGAGTTAGTGTTTT+TGG - chr8.2:22947975-22947994 MS.gene61258:intron 35.0%
! TTGTCATACAGGTCTGAAAT+TGG - chr8.2:22947524-22947543 MS.gene61258:intron 35.0%
!! CTGAAATTTCTAGGATCTTC+TGG + chr8.2:22948083-22948102 None:intergenic 35.0%
!! TCTGGTTGGTAGAGAATAAT+AGG - chr8.2:22948195-22948214 MS.gene61258:intron 35.0%
AATCAGCCATTAAAGGAACC+TGG - chr8.2:22946224-22946243 MS.gene61258:CDS 40.0%
ACCTCAGGAGTAATGTATGA+AGG + chr8.2:22947778-22947797 None:intergenic 40.0%
AGTCATTGTCTTGGAGTATG+CGG - chr8.2:22947206-22947225 MS.gene61258:intron 40.0%
ATCTTCACTAAATCGTCCAG+CGG + chr8.2:22947265-22947284 None:intergenic 40.0%
CAAAGATAAAACAGGACCCT+TGG - chr8.2:22948582-22948601 MS.gene61258:intron 40.0%
CAACAAAGATGCGAGATAGA+AGG + chr8.2:22948272-22948291 None:intergenic 40.0%
CACCATCCCAAAGATAAAAC+AGG - chr8.2:22948574-22948593 MS.gene61258:intron 40.0%
GAATGAGATATCTGTTCTGC+AGG - chr8.2:22947161-22947180 MS.gene61258:intron 40.0%
GACAATGACTAGATGAGAAG+GGG + chr8.2:22947196-22947215 None:intergenic 40.0%
GATAATAACCAATTGCGCCA+AGG + chr8.2:22946821-22946840 None:intergenic 40.0%
GTACATAGAGAGGGGTAAAA+AGG - chr8.2:22946298-22946317 MS.gene61258:CDS 40.0%
GTTATGAGCCATAACAAGTG+GGG + chr8.2:22947877-22947896 None:intergenic 40.0%
GTTATGGCTCATAACAACCA+TGG - chr8.2:22947882-22947901 MS.gene61258:intron 40.0%
TAACTCTGATTCTCTCTGGT+TGG - chr8.2:22948181-22948200 MS.gene61258:intron 40.0%
TCCTGAGGTTCTGTTACAAA+AGG - chr8.2:22947789-22947808 MS.gene61258:CDS 40.0%
TCCTTCATACATTACTCCTG+AGG - chr8.2:22947774-22947793 MS.gene61258:CDS 40.0%
TCGATTACTCCAAGGTTAAC+AGG - chr8.2:22947596-22947615 MS.gene61258:CDS 40.0%
TGAGTTGTGATTGTGTTACC+AGG - chr8.2:22946522-22946541 MS.gene61258:intron 40.0%
TGCTCTCAAGTACATAGAGA+GGG - chr8.2:22946289-22946308 MS.gene61258:CDS 40.0%
TGGACGATTTAGTGAAGATG+AGG - chr8.2:22947266-22947285 MS.gene61258:intron 40.0%
TGTATCATCTGTAGTTCCCT+TGG - chr8.2:22946801-22946820 MS.gene61258:intron 40.0%
TGTGGTTAATTATGTCCCTC+TGG + chr8.2:22946561-22946580 None:intergenic 40.0%
TTGCTCTCAAGTACATAGAG+AGG - chr8.2:22946288-22946307 MS.gene61258:CDS 40.0%
! AACATAAAGTGTCACACCAC+AGG + chr8.2:22948030-22948049 None:intergenic 40.0%
! CAAGGGTCCTGTTTTATCTT+TGG + chr8.2:22948584-22948603 None:intergenic 40.0%
! CTGTTACAAAAGGAGTACGA+CGG - chr8.2:22947799-22947818 MS.gene61258:CDS 40.0%
! GTTTCAAACCCCACTTGTTA+TGG - chr8.2:22947866-22947885 MS.gene61258:intron 40.0%
! TCCTTTTGTAACAGAACCTC+AGG + chr8.2:22947793-22947812 None:intergenic 40.0%
! TGAAATGTGACCGACTCATA+AGG - chr8.2:22946771-22946790 MS.gene61258:CDS 40.0%
!! GGATCTTCTGGTTCTTCAAA+TGG + chr8.2:22948071-22948090 None:intergenic 40.0%
!! GTCCTGTTTTATCTTTGGGA+TGG + chr8.2:22948579-22948598 None:intergenic 40.0%
!! TTAGAGGCGTTTCTTTACCA+CGG + chr8.2:22948448-22948467 None:intergenic 40.0%
!!! ATCTTCGACGTTTTTGGAAC+TGG + chr8.2:22948768-22948787 None:intergenic 40.0%
AATCACACTCAGCAGCACTA+TGG - chr8.2:22946393-22946412 MS.gene61258:CDS 45.0%
ACATTGGTACATACCTTGGC+AGG + chr8.2:22948300-22948319 None:intergenic 45.0%
ACTCAACCAAAGGCAACAGT+TGG - chr8.2:22947748-22947767 MS.gene61258:CDS 45.0%
CAAAAACGTCGAAGATGACG+AGG - chr8.2:22948772-22948791 MS.gene61258:CDS 45.0%
GAGGATCATACAAGAAGCGA+GGG - chr8.2:22948703-22948722 MS.gene61258:CDS 45.0%
GCTCTCAAGTACATAGAGAG+GGG - chr8.2:22946290-22946309 MS.gene61258:CDS 45.0%
TCGTTGACGGGAAGTTAGTT+TGG - chr8.2:22946839-22946858 MS.gene61258:intron 45.0%
TGAGGATCATACAAGAAGCG+AGG - chr8.2:22948702-22948721 MS.gene61258:CDS 45.0%
TTGTCTTTAGCCAACCTAGC+AGG + chr8.2:22946251-22946270 None:intergenic 45.0%
! AACAAGTGGGGTTTGAAACG+TGG + chr8.2:22947865-22947884 None:intergenic 45.0%
! AGAGCGTTATGAGCCATTAG+AGG - chr8.2:22946184-22946203 MS.gene61258:CDS 45.0%
! CTCGTCATCTTCGACGTTTT+TGG + chr8.2:22948774-22948793 None:intergenic 45.0%
! TAGTGCTGCTGAGTGTGATT+AGG + chr8.2:22946393-22946412 None:intergenic 45.0%
!! CGTCTACACTTTGGATTGGT+TGG + chr8.2:22948676-22948695 None:intergenic 45.0%
!! CTGCTGGTGAACTTTTCAAG+AGG - chr8.2:22947232-22947251 MS.gene61258:intron 45.0%
ATTGCAGATGTGTGGTCCTG+TGG - chr8.2:22948011-22948030 MS.gene61258:intron 50.0%
CATTGTCTTGGAGTATGCGG+CGG - chr8.2:22947209-22947228 MS.gene61258:intron 50.0%
GCAGGACCAGGTTCCTTTAA+TGG + chr8.2:22946233-22946252 None:intergenic 50.0%
GGAGATCCAACTGTTGCCTT+TGG + chr8.2:22947757-22947776 None:intergenic 50.0%
GGTACATACCTTGGCAGGAT+CGG + chr8.2:22948295-22948314 None:intergenic 50.0%
TAAAGGAACCTGGTCCTGCT+AGG - chr8.2:22946234-22946253 MS.gene61258:CDS 50.0%
TCTGGACACTCTCATCAACC+TGG + chr8.2:22946543-22946562 None:intergenic 50.0%
TGTAGTTCCCTTGGCGCAAT+TGG - chr8.2:22946810-22946829 MS.gene61258:intron 50.0%
! ACAAAAGGAGTACGACGGCA+AGG - chr8.2:22947804-22947823 MS.gene61258:CDS 50.0%
! CATACAAGAAGCGAGGGTAC+CGG - chr8.2:22948709-22948728 MS.gene61258:CDS 50.0%
!! AGGTTGATGAGAGTGTCCAG+AGG - chr8.2:22946542-22946561 MS.gene61258:intron 50.0%
!! ATACAAGAAGCGAGGGTACC+GGG - chr8.2:22948710-22948729 MS.gene61258:CDS 50.0%
!! GGTTGATGAGAGTGTCCAGA+GGG - chr8.2:22946543-22946562 MS.gene61258:intron 50.0%
CAAGGATCGAAAGCTGGCGA+TGG - chr8.2:22948734-22948753 MS.gene61258:CDS 55.0%
CCAGCTTTCGATCCTTGTCC+CGG + chr8.2:22948731-22948750 None:intergenic 55.0%
CTTTGTTGCCGATCCTGCCA+AGG - chr8.2:22948284-22948303 MS.gene61258:intron 55.0%
TTAGCCAACCTAGCAGGACC+AGG + chr8.2:22946245-22946264 None:intergenic 55.0%
TTCAAGAGGATACGCACCGC+TGG - chr8.2:22947246-22947265 MS.gene61258:intron 55.0%
CCGGGACAAGGATCGAAAGC+TGG - chr8.2:22948728-22948747 MS.gene61258:CDS 60.0%
GGAACCTGGTCCTGCTAGGT+TGG - chr8.2:22946238-22946257 MS.gene61258:CDS 60.0%
TCGAAAGCTGGCGATGGTGC+TGG - chr8.2:22948740-22948759 MS.gene61258:CDS 60.0%
TTGGAGTATGCGGCGGCTGC+TGG - chr8.2:22947216-22947235 MS.gene61258:intron 65.0%
!! GAAGCGAGGGTACCGGGACA+AGG - chr8.2:22948716-22948735 MS.gene61258:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 22946179 22948829 22946179 ID=MS.gene61258
chr8.2 mRNA 22946179 22948829 22946179 ID=MS.gene61258.t1;Parent=MS.gene61258
chr8.2 exon 22948689 22948829 22948689 ID=MS.gene61258.t1.exon1;Parent=MS.gene61258.t1
chr8.2 CDS 22948689 22948829 22948689 ID=cds.MS.gene61258.t1;Parent=MS.gene61258.t1
chr8.2 exon 22948390 22948464 22948390 ID=MS.gene61258.t1.exon2;Parent=MS.gene61258.t1
chr8.2 CDS 22948390 22948464 22948390 ID=cds.MS.gene61258.t1;Parent=MS.gene61258.t1
chr8.2 exon 22947721 22947825 22947721 ID=MS.gene61258.t1.exon3;Parent=MS.gene61258.t1
chr8.2 CDS 22947721 22947825 22947721 ID=cds.MS.gene61258.t1;Parent=MS.gene61258.t1
chr8.2 exon 22947562 22947615 22947562 ID=MS.gene61258.t1.exon4;Parent=MS.gene61258.t1
chr8.2 CDS 22947562 22947615 22947562 ID=cds.MS.gene61258.t1;Parent=MS.gene61258.t1
chr8.2 exon 22946893 22946997 22946893 ID=MS.gene61258.t1.exon5;Parent=MS.gene61258.t1
chr8.2 CDS 22946893 22946997 22946893 ID=cds.MS.gene61258.t1;Parent=MS.gene61258.t1
chr8.2 exon 22946703 22946801 22946703 ID=MS.gene61258.t1.exon6;Parent=MS.gene61258.t1
chr8.2 CDS 22946703 22946801 22946703 ID=cds.MS.gene61258.t1;Parent=MS.gene61258.t1
chr8.2 exon 22946179 22946439 22946179 ID=MS.gene61258.t1.exon7;Parent=MS.gene61258.t1
chr8.2 CDS 22946179 22946439 22946179 ID=cds.MS.gene61258.t1;Parent=MS.gene61258.t1
Gene Sequence

>MS.gene61258

ATGGAAGAGCGTTATGAGCCATTAGAGGAACTTTTTTATTTTTTGAATCAGCCATTAAAGGAACCTGGTCCTGCTAGGTTGGCTAAAGACAAAAATACGGGCGAGCTTGTTGCTCTCAAGTACATAGAGAGGGGTAAAAAGGTAAACATTTCATAAGTTTCCTCATTGAATTGATATTTTGCTGCACTCTTTGTCAAGCTTAAGTAGCTTGCCTAATCACACTCAGCAGCACTATGGAGAAGTGTAGTGTATTAACAAAGCTTAATTATCTTAAACTCTTCATTAAGGATATTATGTACAAGAAGTTTCAATCAAATTCTTTCTGATATGTGTTTGTTCTAAGTGAGTTGTGATTGTGTTACCAGGTTGATGAGAGTGTCCAGAGGGACATAATTAACCACAGATCATTAAAGCATCCTAATATAATCAGATTCAAAGAGGTAACAGTTTTTCTTTCTTAATCTGTTTCTTGTTTAGGTTTGTAGGGGCATTATGAATGTAGTATTTGGTTATCTGTATTACTTCATGTTTACTGCTCTCATTTTACACATTTCTTTAAACTTGTCTCGAAATAGATTTGATTTATTCATAATGAAATGTGACCGACTCATAAGGAAAATCATGTATCATCTGTAGTTCCCTTGGCGCAATTGGTTATTATCGTTGACGGGAAGTTAGTTTGGATAATTTTCTTTTGCTAATTTCTATAGAAAAATATATTTTAAATGATCTTACAGTAACCATGAGTATTTTAGCTATTAGCATCTTTCATAAGCTGAAATTAATTTTTGCACCCAAATTTTTCCAAAAATTCTCTTTTTAGCTTCTTAGTATAACACCGGATCGAAATTTGATCATTCTGCGCAAAAAGTTATATGGTGTAGTGCATCATTACTAAAAGAAAGGTCATAATCATTTGCATTAGTGAAGCTAGATTCCAATTTGTATTATTGAGAGCTTTTAGAAGTTATAACAAAATGATGAATGAGATATCTGTTCTGCAGGTCTTGCTTACCCCTTCTCATCTAGTCATTGTCTTGGAGTATGCGGCGGCTGCTGGTGAACTTTTCAAGAGGATACGCACCGCTGGACGATTTAGTGAAGATGAGGTTATAGAATTAATGTGTTTGAATTGAACTGTTGTTTTTCCTTTTCACGAAAATCGTATACACGAATAAGTTATTCGCATTGAAATTTTCCTATATTTGTTACAGGCAAGATTTTTATTCCAACAGCTAATATCTGGTGTTAGCTACTGTCATTCAATGGTATGCATGCACTTAGAATTCATTGCGTTCTTCATTAAAAGCTCATGTCTTACATTATGCTTTGTGCTGTAGGAAATTTGTCATACAGGTCTGAAATTGGAAAACACTCTTGGATGGAAATCCATCCCCTCAGCTTAAAATTTTCAACTTCGATTACTCCAAGGTTAACAGGATTTCACATAAAATGATCCTTTTTGGATATATATCATATTATGATATCTTATATTATATCCAAGGTTAATAGTATTTGTTAATTTTATTCATCAAATTCAATATTCAATTGCAGTCAACAATATTGCACACTCAACCAAAGGCAACAGTTGGATCTCCTTCATACATTACTCCTGAGGTTCTGTTACAAAAGGAGTACGACGGCAAGGTGCCTGAGATTTTCTCAATCATTTTTGATTAAAAACCACGTTTCAAACCCCACTTGTTATGGCTCATAACAACCATGGTTTAACAATTGACAAATAGTAATAGTACAGTCTCGTATTGATTAAAAAGTTCACGTTTTGGTCAAAAATTGTCGATTGAGTTAGTGTTTTTGGTTCTTTTGTATAGATTGCAGATGTGTGGTCCTGTGGTGTGACACTTTATGTTATGCTAGTTGGTGCATATCCATTTGAAGAACCAGAAGATCCTAGAAATTTCAGAAAGACTATTGGGGTGAGTACTTATTTAACTTGACTTCAAAAATGTCATTATGAATGATTTCTGATACCAGGCAATTTTAACTCTGATTCTCTCTGGTTGGTAGAGAATAATAGGTGTTCAATACTCCATACCCGACAATGTACATATTTCAGTCGAGTGTAGGAACCTTCTATCTCGCATCTTTGTTGCCGATCCTGCCAAGGTATGTACCAATGTAACATTGACTGATGTTATTAAAAGATACAAACATTATACTTATATTATGCATACCACCTATAGCATTAGAATTACAAATATTCATGGTAATTGCCTAAGAAATGGAAAATAATGTAACTAATCATCCGTGGTAAAGAAACGCCTCTAACATCACTGATAGAATTAATGCAAAAAAAGGAGTTAGATTATATATTTTATAATGAGATTATGTTTCTTTTTCAAACTAATTATTTCTTATACTTGATAAAGAGGATCACCATCCCAAAGATAAAACAGGACCCTTGGTTTTTGAAGAATTTGCCTGAAGAGATAATTGAAGCTGAAAGAAAATGTTATGTGGAAACAAAGAAGGACCAACCAATCCAAAGTGTAGACGAAATCATGAGGATCATACAAGAAGCGAGGGTACCGGGACAAGGATCGAAAGCTGGCGATGGTGCTGGCACCAGTTCCAAAAACGTCGAAGATGACGAGGAAATTGATATCAGGGATGACTATGAAAATGTGTGA
Protein sequence

>MS.gene61258.t1

MEERYEPLEELFYFLNQPLKEPGPARLAKDKNTGELVALKYIERGKKVDESVQRDIINHRSLKHPNIIRFKEVLLTPSHLVIVLEYAAAAGELFKRIRTAGRFSEDEARFLFQQLISGVSYCHSMIADVWSCGVTLYVMLVGAYPFEEPEDPRNFRKTIGRIIGVQYSIPDNVHISVECRNLLSRIFVADPAKRITIPKIKQDPWFLKNLPEEIIEAERKCYVETKKDQPIQSVDEIMRIIQEARVPGQGSKAGDGAGTSSKNVEDDEEIDIRDDYENV