AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene61473

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61473.t1 MTR_4g113860 86.293 321 44 0 1 321 1 321 0.0 581
MS.gene61473.t1 MTR_4g113970 59.924 262 83 6 1 248 1 254 1.80e-110 321
MS.gene61473.t1 MTR_4g113940 49.351 308 117 6 1 307 1 270 5.66e-97 288
MS.gene61473.t1 MTR_3g050390 48.529 272 101 8 51 320 7 241 2.34e-78 239
MS.gene61473.t1 MTR_4g113890 41.479 311 96 6 1 306 1 230 1.05e-71 222
MS.gene61473.t1 MTR_8g066730 28.094 299 140 6 77 307 83 374 1.67e-27 110
MS.gene61473.t1 MTR_8g066730 29.717 212 98 4 77 244 83 287 4.53e-21 91.7
MS.gene61473.t1 MTR_3g466460 57.143 84 33 2 233 315 21 102 2.50e-18 80.5
MS.gene61473.t1 MTR_4g113980 60.870 69 23 3 241 307 24 90 3.06e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61473.t1 AT3G19360 35.838 346 154 8 32 310 32 376 1.60e-62 203
MS.gene61473.t1 AT3G19360 35.838 346 154 8 32 310 32 376 1.60e-62 203
MS.gene61473.t1 AT1G32360 27.304 293 146 5 82 307 90 382 3.54e-22 96.3
MS.gene61473.t1 AT2G35430 26.667 195 108 6 138 307 66 250 4.50e-12 65.5

Find 91 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAGATCAATCGTGTTTA+TGG 0.098083 8.3:-13447618 MS.gene61473:CDS
GACAGCAGCAGCTGAGAAAT+TGG 0.269527 8.3:-13449659 MS.gene61473:CDS
TCCTTTGCCATAAGTCTTGC+AGG 0.273145 8.3:+13447684 None:intergenic
ATGAGGATCCTGCTAAGTTT+AGG 0.284721 8.3:-13449542 MS.gene61473:CDS
TCTATAAGCATTGGGGCTAT+TGG 0.301858 8.3:-13449489 MS.gene61473:CDS
TAAGACTGCCACCACGAAAT+TGG 0.308491 8.3:-13449710 MS.gene61473:CDS
ATTGGTTCTTCAAAGTCATT+TGG 0.320226 8.3:-13449471 MS.gene61473:CDS
GATGGAGGAAGAACCATCTT+TGG 0.330810 8.3:+13449840 None:intergenic
TGAGGATCCTGCTAAGTTTA+GGG 0.331108 8.3:-13449541 MS.gene61473:CDS
AATGGTGAGAAATGTTTCTT+TGG 0.334123 8.3:-13449585 MS.gene61473:CDS
CTAAGTTTAGGGATCATTCT+TGG 0.335825 8.3:-13449530 MS.gene61473:CDS
CACCTTCCCTGCAAGACTTA+TGG 0.338047 8.3:-13447691 MS.gene61473:CDS
AGCCAGCTAGGGGCACTTAT+TGG 0.348926 8.3:-13449425 MS.gene61473:CDS
ACTTCACGAAAAGCCTTAAC+AGG 0.358557 8.3:-13447741 MS.gene61473:CDS
TTGGTTCTTCAAAGTCATTT+GGG 0.358694 8.3:-13449470 MS.gene61473:CDS
TCAATCGTGTTTATGGTGAT+TGG 0.369324 8.3:-13447611 MS.gene61473:CDS
ATCGAGGGAGATGTCCCTTT+GGG 0.370733 8.3:-13449374 MS.gene61473:CDS
TTGCGGCGGTGACATATTAC+AGG 0.379182 8.3:+13450019 None:intergenic
ATCAAACCCATTCCTTCAAA+AGG 0.395378 8.3:-13449911 MS.gene61473:CDS
AGTGTTCTTCTATAAGCATT+GGG 0.403399 8.3:-13449497 MS.gene61473:CDS
ACGTGGTCGGCTCTGTTTGA+TGG 0.413337 8.3:+13449822 None:intergenic
CTAAGAAGATGCATTTACTT+TGG 0.420995 8.3:-13447650 MS.gene61473:CDS
GGGCTGTGGACAAGCCAGCT+AGG 0.423889 8.3:-13449437 MS.gene61473:CDS
AAAGAAACATTTCTCACCAT+TGG 0.440730 8.3:+13449587 None:intergenic
GGCTGTGGACAAGCCAGCTA+GGG 0.459838 8.3:-13449436 MS.gene61473:CDS
TTACAGGTTGGAATATTGTC+TGG 0.459849 8.3:+13450035 None:intergenic
AGTCATTTGGGGATGGCACT+AGG 0.462888 8.3:-13449458 MS.gene61473:CDS
TGCCATAAGTCTTGCAGGGA+AGG 0.474061 8.3:+13447689 None:intergenic
CATCGAGGGAGATGTCCCTT+TGG 0.474158 8.3:-13449375 MS.gene61473:CDS
TTCCAATAAGTGCCCCTAGC+TGG 0.475908 8.3:+13449423 None:intergenic
ACAGCAGCAGCTGAGAAATT+GGG 0.480050 8.3:-13449658 MS.gene61473:CDS
GATATGCCCTCCTCCAAAGA+TGG 0.484001 8.3:-13449853 MS.gene61473:CDS
AAAGATTGCAACTTTGCTCA+TGG 0.488168 8.3:-13449744 MS.gene61473:CDS
TGGGGATGGCACTAGGGCTG+TGG 0.491393 8.3:-13449451 MS.gene61473:CDS
GTTTCTACAAGTACCAAGTC+TGG 0.493005 8.3:+13449770 None:intergenic
AAGATTGCAACTTTGCTCAT+GGG 0.501520 8.3:-13449743 MS.gene61473:CDS
AGTCAGTTCTCCACTGCACT+TGG 0.503719 8.3:+13447555 None:intergenic
GAATGATCCCTAAACTTAGC+AGG 0.506220 8.3:+13449534 None:intergenic
TCCACTGCACTTGGCAAGGT+GGG 0.506459 8.3:+13447564 None:intergenic
TTCACAAGATGAAGCTATGC+AGG 0.506731 8.3:-13449617 MS.gene61473:CDS
CAATTTGCTCATGGAGAAGC+AGG 0.507401 8.3:-13449342 MS.gene61473:intron
AGACTAAGACGTGTTTCCAT+TGG 0.510007 8.3:-13449401 MS.gene61473:CDS
ATCTCCCTCGATGCTGCCAA+TGG 0.511208 8.3:+13449385 None:intergenic
TGGTAGGTGCATATGCATCA+TGG 0.515067 8.3:+13447712 None:intergenic
GAGTGTTCTTCTATAAGCAT+TGG 0.515583 8.3:-13449498 MS.gene61473:CDS
AGACTTGGTACTTGTAGAAA+CGG 0.521320 8.3:-13449768 MS.gene61473:CDS
AAGACTTATGGCAAAGGAAG+AGG 0.525073 8.3:-13447679 MS.gene61473:CDS
ATCATGGGCAGAACCTGTTA+AGG 0.525153 8.3:+13447728 None:intergenic
GAGGACTGTCAATTTGCTCA+TGG 0.526443 8.3:-13449351 MS.gene61473:CDS
GTTTCCATTGGCAGCATCGA+GGG 0.527680 8.3:-13449389 MS.gene61473:CDS
TATGGCAAAGGAAGAGGAGC+AGG 0.527945 8.3:-13447673 MS.gene61473:CDS
ACTAGCTCCTGCCAATTTCG+TGG 0.541649 8.3:+13449699 None:intergenic
TCTTCAAAGTCATTTGGGGA+TGG 0.542249 8.3:-13449465 MS.gene61473:CDS
TCCCACCTTGCCAAGTGCAG+TGG 0.542507 8.3:-13447565 MS.gene61473:CDS
GTCATTTGGGGATGGCACTA+GGG 0.548609 8.3:-13449457 MS.gene61473:CDS
AGATTGCAACTTTGCTCATG+GGG 0.548831 8.3:-13449742 MS.gene61473:CDS
TCAAACCCATTCCTTCAAAA+GGG 0.549206 8.3:-13449910 MS.gene61473:CDS
AGGAGGGCATATCAAAGCAT+TGG 0.555491 8.3:+13449863 None:intergenic
ACTGCCACCACGAAATTGGC+AGG 0.556769 8.3:-13449706 MS.gene61473:CDS
GGAAGAACCATCTTTGGAGG+AGG 0.559899 8.3:+13449846 None:intergenic
AATTGACAGTCCTCCCCAAA+GGG 0.560613 8.3:+13449360 None:intergenic
GTTCTCCACTGCACTTGGCA+AGG 0.561112 8.3:+13447560 None:intergenic
GGAGGAAGAACCATCTTTGG+AGG 0.566069 8.3:+13449843 None:intergenic
GGTAGGTGCATATGCATCAT+GGG 0.568261 8.3:+13447713 None:intergenic
ATCTGTAATAAGTCCAGACT+TGG 0.576422 8.3:-13449783 MS.gene61473:CDS
TGCTGCTGTCCGTCGCTGCG+AGG 0.579246 8.3:+13449672 None:intergenic
CCTTTGCCATAAGTCTTGCA+GGG 0.581829 8.3:+13447685 None:intergenic
CATAAGTCTTGCAGGGAAGG+TGG 0.582500 8.3:+13447692 None:intergenic
CTCCACTGCACTTGGCAAGG+TGG 0.585446 8.3:+13447563 None:intergenic
CCCTGCAAGACTTATGGCAA+AGG 0.589645 8.3:-13447685 MS.gene61473:CDS
AGTCTTGCAGGGAAGGTGGT+AGG 0.597135 8.3:+13447696 None:intergenic
TGGTTCTTCAAAGTCATTTG+GGG 0.598253 8.3:-13449469 MS.gene61473:CDS
GGCAAGGTGGGAAGAACAAG+AGG 0.612688 8.3:+13447576 None:intergenic
CTAGTTAGTCCTCGCAGCGA+CGG 0.619159 8.3:-13449681 MS.gene61473:CDS
AAATTGACAGTCCTCCCCAA+AGG 0.626382 8.3:+13449359 None:intergenic
GGCGGTGACATATTACAGGT+TGG 0.626507 8.3:+13450023 None:intergenic
CTGTTTGATGTAGTGAATTG+CGG 0.628943 8.3:+13450002 None:intergenic
GAAGAACCATCTTTGGAGGA+GGG 0.630074 8.3:+13449847 None:intergenic
AGGGAGATGTCCCTTTGGGG+AGG 0.650401 8.3:-13449370 MS.gene61473:CDS
TGGTCGGCTCTGTTTGATGG+AGG 0.652720 8.3:+13449825 None:intergenic
CTATGCAGGATGTATTCCAA+TGG 0.653635 8.3:-13449603 MS.gene61473:CDS
GTGTTCTTCTATAAGCATTG+GGG 0.654358 8.3:-13449496 MS.gene61473:CDS
GCTGTGGACAAGCCAGCTAG+GGG 0.660887 8.3:-13449435 MS.gene61473:CDS
TCGAGGGAGATGTCCCTTTG+GGG 0.664726 8.3:-13449373 MS.gene61473:CDS
AGCTCCTGCCAATTTCGTGG+TGG 0.669372 8.3:+13449702 None:intergenic
TGTTTCCATTGGCAGCATCG+AGG 0.674096 8.3:-13449390 MS.gene61473:CDS
GCAGCAGCTGAGAAATTGGG+AGG 0.680326 8.3:-13449655 MS.gene61473:CDS
TTTGATGTAGTGAATTGCGG+CGG 0.699237 8.3:+13450005 None:intergenic
AATATTGTCTGGAAGAACCA+TGG 0.708668 8.3:+13450046 None:intergenic
TACGAGTCTTGTAGAAAACG+TGG 0.711272 8.3:+13449805 None:intergenic
AGTCTTGTAGAAAACGTGGT+CGG 0.717485 8.3:+13449809 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGATGAATATAACATAA+TGG + chr8.3:13449438-13449457 None:intergenic 15.0%
!! CAAAATCAATAATCAAAATA+GGG + chr8.3:13448320-13448339 None:intergenic 15.0%
!! CAAGCAATATATATAAAAAA+TGG + chr8.3:13449694-13449713 None:intergenic 15.0%
!! TCGGATAAAAAAAAAAAATT+AGG - chr8.3:13449324-13449343 MS.gene61473:intron 15.0%
!!! ATCCATTTACAAATTTTTTA+TGG + chr8.3:13448929-13448948 None:intergenic 15.0%
!!! ATTTTACAATTTAAACTGTT+TGG - chr8.3:13449765-13449784 MS.gene61473:CDS 15.0%
!!! TTAATCATCTCAATTTTATA+AGG - chr8.3:13449147-13449166 MS.gene61473:intron 15.0%
!! AAAGATAAAATAATCACTAG+TGG + chr8.3:13448710-13448729 None:intergenic 20.0%
!! AATAATCAAAATAGGGAAAA+GGG + chr8.3:13448313-13448332 None:intergenic 20.0%
!! AGCCATAAAAAATTTGTAAA+TGG - chr8.3:13448924-13448943 MS.gene61473:intron 20.0%
!! ATATGATGTTCAAATTATAG+TGG - chr8.3:13448434-13448453 MS.gene61473:intron 20.0%
!! CCAAAATCAATAATCAAAAT+AGG + chr8.3:13448321-13448340 None:intergenic 20.0%
!!! CCTATTTTGATTATTGATTT+TGG - chr8.3:13448318-13448337 MS.gene61473:intron 20.0%
!!! GTGTTTATATGTTGTATTTT+TGG - chr8.3:13448982-13449001 MS.gene61473:intron 20.0%
! AAAACTGTGTTGTCAAATAT+CGG - chr8.3:13448544-13448563 MS.gene61473:intron 25.0%
! AAGGCATGATTACTATTAAA+TGG - chr8.3:13449166-13449185 MS.gene61473:intron 25.0%
! CAATAATCAAAATAGGGAAA+AGG + chr8.3:13448314-13448333 None:intergenic 25.0%
! CATATTTGACTGCAATATTA+AGG + chr8.3:13449584-13449603 None:intergenic 25.0%
! CTTAATATTGCAGTCAAATA+TGG - chr8.3:13449582-13449601 MS.gene61473:CDS 25.0%
! GATATGTAAACCTTAGATAA+AGG + chr8.3:13448850-13448869 None:intergenic 25.0%
! TCAAATAGAAATTCCTAATG+TGG + chr8.3:13449095-13449114 None:intergenic 25.0%
! TCCAATGAAAAATGAAAACT+TGG - chr8.3:13447649-13447668 MS.gene61473:CDS 25.0%
! TGAGTTAGACATCAAATTAT+AGG + chr8.3:13449501-13449520 None:intergenic 25.0%
!! TACATATCAGTGTTGTTAAA+TGG - chr8.3:13448862-13448881 MS.gene61473:intron 25.0%
!! TATATGTCGATGGATATTTT+GGG - chr8.3:13449361-13449380 MS.gene61473:CDS 25.0%
!! TATTTCTATGTATTGGATCA+CGG + chr8.3:13449394-13449413 None:intergenic 25.0%
!! TCACATGTATTTCTATGTAT+TGG + chr8.3:13449401-13449420 None:intergenic 25.0%
!!! TAAGTGTTATTTTCTTCATG+AGG - chr8.3:13448042-13448061 MS.gene61473:intron 25.0%
!!! TCCAAGTTTTCATTTTTCAT+TGG + chr8.3:13447653-13447672 None:intergenic 25.0%
!!! TGACTGAAATTTTAGTAGTT+TGG - chr8.3:13449213-13449232 MS.gene61473:intron 25.0%
AAAGAAACATTTCTCACCAT+TGG + chr8.3:13448017-13448036 None:intergenic 30.0%
AACAAGATCAATCGTGTTTA+TGG - chr8.3:13449983-13450002 MS.gene61473:CDS 30.0%
AATGGTGAGAAATGTTTCTT+TGG - chr8.3:13448016-13448035 MS.gene61473:intron 30.0%
AGTGTTCTTCTATAAGCATT+GGG - chr8.3:13448104-13448123 MS.gene61473:intron 30.0%
ATCATATAATTCTGCTGTAC+TGG - chr8.3:13448345-13448364 MS.gene61473:intron 30.0%
CAATATTAAGGATTGTGACA+TGG + chr8.3:13449572-13449591 None:intergenic 30.0%
CTAAGAAGATGCATTTACTT+TGG - chr8.3:13449951-13449970 MS.gene61473:CDS 30.0%
CTTTATAGCTGATAACAGAA+AGG + chr8.3:13449258-13449277 None:intergenic 30.0%
TCTTTGTGTTCAAATTTCCT+TGG - chr8.3:13448725-13448744 MS.gene61473:intron 30.0%
TGACAACTGCTATGTTTATA+TGG - chr8.3:13448776-13448795 MS.gene61473:intron 30.0%
TGATAACACTCCTTTATCTA+AGG - chr8.3:13448837-13448856 MS.gene61473:intron 30.0%
TGTTATCAGCTATAAAGCAT+GGG - chr8.3:13449261-13449280 MS.gene61473:intron 30.0%
! ATTGGTTCTTCAAAGTCATT+TGG - chr8.3:13448130-13448149 MS.gene61473:intron 30.0%
! GCGTATGATATTTTCATGTT+TGG - chr8.3:13448482-13448501 MS.gene61473:intron 30.0%
! GTATATGTCGATGGATATTT+TGG - chr8.3:13449360-13449379 MS.gene61473:CDS 30.0%
! TTGGTTCTTCAAAGTCATTT+GGG - chr8.3:13448131-13448150 MS.gene61473:intron 30.0%
!! CTAACTCATCAATAGTTTTG+AGG - chr8.3:13449513-13449532 MS.gene61473:CDS 30.0%
!! TCATTTTTCATTGGAAATCG+AGG + chr8.3:13447644-13447663 None:intergenic 30.0%
!!! ACGTTGAGGTTTTTTAATTG+AGG + chr8.3:13449647-13449666 None:intergenic 30.0%
AAAATTAGGATGCTGCACTT+TGG - chr8.3:13449338-13449357 MS.gene61473:intron 35.0%
AAAGATTGCAACTTTGCTCA+TGG - chr8.3:13447857-13447876 MS.gene61473:intron 35.0%
AAGATTGCAACTTTGCTCAT+GGG - chr8.3:13447858-13447877 MS.gene61473:intron 35.0%
ACCAAAAGGAAAAACAGGTT+TGG + chr8.3:13449461-13449480 None:intergenic 35.0%
ATCAAACCCATTCCTTCAAA+AGG - chr8.3:13447690-13447709 MS.gene61473:CDS 35.0%
ATCTGTAATAAGTCCAGACT+TGG - chr8.3:13447818-13447837 MS.gene61473:intron 35.0%
ATGAGTCAGAGTATGATACA+TGG + chr8.3:13449794-13449813 None:intergenic 35.0%
CAACTGCTATGTTTATATGG+CGG - chr8.3:13448779-13448798 MS.gene61473:intron 35.0%
CAGAAACCAAAAGGAAAAAC+AGG + chr8.3:13449466-13449485 None:intergenic 35.0%
CTAAGTTTAGGGATCATTCT+TGG - chr8.3:13448071-13448090 MS.gene61473:intron 35.0%
CTGTTATCAGCTATAAAGCA+TGG - chr8.3:13449260-13449279 MS.gene61473:intron 35.0%
CTGTTTGATGTAGTGAATTG+CGG + chr8.3:13447602-13447621 None:intergenic 35.0%
GAGTGTTCTTCTATAAGCAT+TGG - chr8.3:13448103-13448122 MS.gene61473:intron 35.0%
GGATGGTTAAATATACTGGA+AGG + chr8.3:13449008-13449027 None:intergenic 35.0%
GTATATTTAACCATCCTCCA+TGG - chr8.3:13449012-13449031 MS.gene61473:intron 35.0%
GTGGCTTAAAATTGCACATT+TGG - chr8.3:13448639-13448658 MS.gene61473:intron 35.0%
TCAAACCCATTCCTTCAAAA+GGG - chr8.3:13447691-13447710 MS.gene61473:CDS 35.0%
TCAATCGTGTTTATGGTGAT+TGG - chr8.3:13449990-13450009 MS.gene61473:CDS 35.0%
TGGAGGATGGTTAAATATAC+TGG + chr8.3:13449012-13449031 None:intergenic 35.0%
TGGTTCTTCAAAGTCATTTG+GGG - chr8.3:13448132-13448151 MS.gene61473:intron 35.0%
! AGAAATTCTTGCCCTTTTGA+AGG + chr8.3:13447705-13447724 None:intergenic 35.0%
! AGACTTGGTACTTGTAGAAA+CGG - chr8.3:13447833-13447852 MS.gene61473:intron 35.0%
! GTGTTCTTCTATAAGCATTG+GGG - chr8.3:13448105-13448124 MS.gene61473:intron 35.0%
! TTACAGGTTGGAATATTGTC+TGG + chr8.3:13447569-13447588 None:intergenic 35.0%
!! TAGGTATCGAAATTGCTTGT+CGG - chr8.3:13449305-13449324 MS.gene61473:intron 35.0%
!! TCCAAACCTGTTTTTCCTTT+TGG - chr8.3:13449457-13449476 MS.gene61473:CDS 35.0%
!! TTTATATGGCGGATTTCAGT+TGG - chr8.3:13448790-13448809 MS.gene61473:intron 35.0%
AAACACACCATTGTGACCTA+TGG + chr8.3:13448968-13448987 None:intergenic 40.0%
AAGACTTATGGCAAAGGAAG+AGG - chr8.3:13449922-13449941 MS.gene61473:CDS 40.0%
ACTGTGTTGTCAAATATCGG+CGG - chr8.3:13448547-13448566 MS.gene61473:intron 40.0%
ACTTCACGAAAAGCCTTAAC+AGG - chr8.3:13449860-13449879 MS.gene61473:CDS 40.0%
AGACTAAGACGTGTTTCCAT+TGG - chr8.3:13448200-13448219 MS.gene61473:intron 40.0%
AGATTGCAACTTTGCTCATG+GGG - chr8.3:13447859-13447878 MS.gene61473:intron 40.0%
AGGTACATTGCTCATTTGCA+AGG - chr8.3:13449533-13449552 MS.gene61473:CDS 40.0%
ATATCAATGCGTTGCTACCA+AGG + chr8.3:13448745-13448764 None:intergenic 40.0%
ATGAGGATCCTGCTAAGTTT+AGG - chr8.3:13448059-13448078 MS.gene61473:intron 40.0%
CTATGCAGGATGTATTCCAA+TGG - chr8.3:13447998-13448017 MS.gene61473:intron 40.0%
GAATGATCCCTAAACTTAGC+AGG + chr8.3:13448070-13448089 None:intergenic 40.0%
GTTTCTACAAGTACCAAGTC+TGG + chr8.3:13447834-13447853 None:intergenic 40.0%
TAAGCCACCATAAAATGCCA+TGG + chr8.3:13448627-13448646 None:intergenic 40.0%
TCTTCAAAGTCATTTGGGGA+TGG - chr8.3:13448136-13448155 MS.gene61473:intron 40.0%
TGAGGATCCTGCTAAGTTTA+GGG - chr8.3:13448060-13448079 MS.gene61473:intron 40.0%
TGCACTTTGGTATATGTCGA+TGG - chr8.3:13449351-13449370 MS.gene61473:CDS 40.0%
TTCACAAGATGAAGCTATGC+AGG - chr8.3:13447984-13448003 MS.gene61473:intron 40.0%
TTTGATGTAGTGAATTGCGG+CGG + chr8.3:13447599-13447618 None:intergenic 40.0%
! AGTCTTGTAGAAAACGTGGT+CGG + chr8.3:13447795-13447814 None:intergenic 40.0%
! ATTTTCGTGTCAGCCACATT+AGG - chr8.3:13449079-13449098 MS.gene61473:intron 40.0%
! GTTAAGGCTTTTCGTGAAGT+TGG + chr8.3:13449860-13449879 None:intergenic 40.0%
! TACGAGTCTTGTAGAAAACG+TGG + chr8.3:13447799-13447818 None:intergenic 40.0%
!! TCTATAAGCATTGGGGCTAT+TGG - chr8.3:13448112-13448131 MS.gene61473:intron 40.0%
!! TCTTGCCCTTTTGAAGGAAT+GGG + chr8.3:13447699-13447718 None:intergenic 40.0%
!! TTCTTGCCCTTTTGAAGGAA+TGG + chr8.3:13447700-13447719 None:intergenic 40.0%
!!! TCCTTTTGGTTTCTGCTGAT+GGG - chr8.3:13449471-13449490 MS.gene61473:CDS 40.0%
!!! TTCCTTTTGGTTTCTGCTGA+TGG - chr8.3:13449470-13449489 MS.gene61473:CDS 40.0%
AAATTGACAGTCCTCCCCAA+AGG + chr8.3:13448245-13448264 None:intergenic 45.0%
AATTGACAGTCCTCCCCAAA+GGG + chr8.3:13448244-13448263 None:intergenic 45.0%
ACAGCAGCAGCTGAGAAATT+GGG - chr8.3:13447943-13447962 MS.gene61473:intron 45.0%
ACCCATCAGCAGAAACCAAA+AGG + chr8.3:13449475-13449494 None:intergenic 45.0%
AGTGCCAAGACAGAATAGTG+TGG - chr8.3:13448886-13448905 MS.gene61473:intron 45.0%
ATCATGGGCAGAACCTGTTA+AGG + chr8.3:13449876-13449895 None:intergenic 45.0%
CAATTGTTGCAGAGACGTTG+AGG + chr8.3:13449661-13449680 None:intergenic 45.0%
CATCCATCACAAATTGGCCA+TGG + chr8.3:13448598-13448617 None:intergenic 45.0%
CATCGCCATCCATCACAAAT+TGG + chr8.3:13448604-13448623 None:intergenic 45.0%
CATGGTGCTTCCTAAAAGTC+TGG + chr8.3:13448580-13448599 None:intergenic 45.0%
CCAGTGTTTACGATGACAGA+TGG + chr8.3:13448672-13448691 None:intergenic 45.0%
CCATCTGTCATCGTAAACAC+TGG - chr8.3:13448669-13448688 MS.gene61473:intron 45.0%
CCTTTGCCATAAGTCTTGCA+GGG + chr8.3:13449919-13449938 None:intergenic 45.0%
GATGGAGGAAGAACCATCTT+TGG + chr8.3:13447764-13447783 None:intergenic 45.0%
GGTAGGTGCATATGCATCAT+GGG + chr8.3:13449891-13449910 None:intergenic 45.0%
GTGTTATCAGTAGCAGATGG+TGG + chr8.3:13448826-13448845 None:intergenic 45.0%
GTGTTTACGATGACAGATGG+TGG + chr8.3:13448669-13448688 None:intergenic 45.0%
TAAGACTGCCACCACGAAAT+TGG - chr8.3:13447891-13447910 MS.gene61473:intron 45.0%
TCCTTTGCCATAAGTCTTGC+AGG + chr8.3:13449920-13449939 None:intergenic 45.0%
TCTGCCACACTATTCTGTCT+TGG + chr8.3:13448893-13448912 None:intergenic 45.0%
TGGTAGGTGCATATGCATCA+TGG + chr8.3:13449892-13449911 None:intergenic 45.0%
! AGGAGGGCATATCAAAGCAT+TGG + chr8.3:13447741-13447760 None:intergenic 45.0%
! CAATTTGCTCATGGAGAAGC+AGG - chr8.3:13448259-13448278 MS.gene61473:intron 45.0%
! CATGGCCAATTTGTGATGGA+TGG - chr8.3:13448596-13448615 MS.gene61473:intron 45.0%
! CGGTCAAATGCCAGACTTTT+AGG - chr8.3:13448567-13448586 MS.gene61473:intron 45.0%
! GAAGAACCATCTTTGGAGGA+GGG + chr8.3:13447757-13447776 None:intergenic 45.0%
! GAGGACTGTCAATTTGCTCA+TGG - chr8.3:13448250-13448269 MS.gene61473:intron 45.0%
! GATGCCATGGCATTTTATGG+TGG - chr8.3:13448620-13448639 MS.gene61473:intron 45.0%
! GGAGTGTTATCAGTAGCAGA+TGG + chr8.3:13448829-13448848 None:intergenic 45.0%
!!! CAGACTTTTAGGAAGCACCA+TGG - chr8.3:13448578-13448597 MS.gene61473:intron 45.0%
ACAGTGTTGTCCATGGAGGA+TGG + chr8.3:13449025-13449044 None:intergenic 50.0%
ACCTACTTGCGTAACCACCA+CGG + chr8.3:13449290-13449309 None:intergenic 50.0%
ACTAGCTCCTGCCAATTTCG+TGG + chr8.3:13447905-13447924 None:intergenic 50.0%
AGCGTCACCATAGGTCACAA+TGG - chr8.3:13448958-13448977 MS.gene61473:intron 50.0%
AGTCATTTGGGGATGGCACT+AGG - chr8.3:13448143-13448162 MS.gene61473:intron 50.0%
AGTGACAGTGTTGTCCATGG+AGG + chr8.3:13449029-13449048 None:intergenic 50.0%
ATCGAGGGAGATGTCCCTTT+GGG - chr8.3:13448227-13448246 MS.gene61473:intron 50.0%
CACAGTGACAGTGTTGTCCA+TGG + chr8.3:13449032-13449051 None:intergenic 50.0%
CACCTTCCCTGCAAGACTTA+TGG - chr8.3:13449910-13449929 MS.gene61473:CDS 50.0%
CATAAGTCTTGCAGGGAAGG+TGG + chr8.3:13449912-13449931 None:intergenic 50.0%
CCCTGCAAGACTTATGGCAA+AGG - chr8.3:13449916-13449935 MS.gene61473:CDS 50.0%
CTATAAAGCATGGGTACCCG+TGG - chr8.3:13449270-13449289 MS.gene61473:intron 50.0%
GACAGCAGCAGCTGAGAAAT+TGG - chr8.3:13447942-13447961 MS.gene61473:intron 50.0%
GATATGCCCTCCTCCAAAGA+TGG - chr8.3:13447748-13447767 MS.gene61473:CDS 50.0%
GCACCATGGCCAATTTGTGA+TGG - chr8.3:13448592-13448611 MS.gene61473:intron 50.0%
GGAGGAAGAACCATCTTTGG+AGG + chr8.3:13447761-13447780 None:intergenic 50.0%
GGCGGTGACATATTACAGGT+TGG + chr8.3:13447581-13447600 None:intergenic 50.0%
GTCATTTGGGGATGGCACTA+GGG - chr8.3:13448144-13448163 MS.gene61473:intron 50.0%
TATGGCAAAGGAAGAGGAGC+AGG - chr8.3:13449928-13449947 MS.gene61473:CDS 50.0%
TGCCATAAGTCTTGCAGGGA+AGG + chr8.3:13449915-13449934 None:intergenic 50.0%
TGTTTCCATTGGCAGCATCG+AGG - chr8.3:13448211-13448230 MS.gene61473:intron 50.0%
TTCCAATAAGTGCCCCTAGC+TGG + chr8.3:13448181-13448200 None:intergenic 50.0%
TTGCGGCGGTGACATATTAC+AGG + chr8.3:13447585-13447604 None:intergenic 50.0%
! GGAAGAACCATCTTTGGAGG+AGG + chr8.3:13447758-13447777 None:intergenic 50.0%
! GGCGATGCCATGGCATTTTA+TGG - chr8.3:13448617-13448636 MS.gene61473:intron 50.0%
! GTTTCCATTGGCAGCATCGA+GGG - chr8.3:13448212-13448231 MS.gene61473:intron 50.0%
!! TAGCAGATGGTGGATCACGA+CGG + chr8.3:13448816-13448835 None:intergenic 50.0%
ACTGCCACCACGAAATTGGC+AGG - chr8.3:13447895-13447914 MS.gene61473:intron 55.0%
AGCTCCTGCCAATTTCGTGG+TGG + chr8.3:13447902-13447921 None:intergenic 55.0%
AGTCTTGCAGGGAAGGTGGT+AGG + chr8.3:13449908-13449927 None:intergenic 55.0%
ATCTCCCTCGATGCTGCCAA+TGG + chr8.3:13448219-13448238 None:intergenic 55.0%
CATCGAGGGAGATGTCCCTT+TGG - chr8.3:13448226-13448245 MS.gene61473:intron 55.0%
CCTACTTGCGTAACCACCAC+GGG + chr8.3:13449289-13449308 None:intergenic 55.0%
CTAGTTAGTCCTCGCAGCGA+CGG - chr8.3:13447920-13447939 MS.gene61473:intron 55.0%
GCAGCAGCTGAGAAATTGGG+AGG - chr8.3:13447946-13447965 MS.gene61473:intron 55.0%
GGCAAGGTGGGAAGAACAAG+AGG + chr8.3:13450028-13450047 None:intergenic 55.0%
GTTCTCCACTGCACTTGGCA+AGG + chr8.3:13450044-13450063 None:intergenic 55.0%
TAAAGCATGGGTACCCGTGG+TGG - chr8.3:13449273-13449292 MS.gene61473:intron 55.0%
TCCACTGCACTTGGCAAGGT+GGG + chr8.3:13450040-13450059 None:intergenic 55.0%
TCGAGGGAGATGTCCCTTTG+GGG - chr8.3:13448228-13448247 MS.gene61473:intron 55.0%
TGCAGCTGCAGTTGCAGTGT+CGG - chr8.3:13449609-13449628 MS.gene61473:CDS 55.0%
! ACGTGGTCGGCTCTGTTTGA+TGG + chr8.3:13447782-13447801 None:intergenic 55.0%
! TGGTCGGCTCTGTTTGATGG+AGG + chr8.3:13447779-13447798 None:intergenic 55.0%
! TGTGACCTATGGTGACGCTG+TGG + chr8.3:13448957-13448976 None:intergenic 55.0%
!! AGCCAGCTAGGGGCACTTAT+TGG - chr8.3:13448176-13448195 MS.gene61473:intron 55.0%
!! TGTGATGGATGGCGATGCCA+TGG - chr8.3:13448607-13448626 MS.gene61473:intron 55.0%
CCCGTGGTGGTTACGCAAGT+AGG - chr8.3:13449286-13449305 MS.gene61473:intron 60.0%
CTCCACTGCACTTGGCAAGG+TGG + chr8.3:13450041-13450060 None:intergenic 60.0%
GCTGTGGACAAGCCAGCTAG+GGG - chr8.3:13448166-13448185 MS.gene61473:intron 60.0%
GGCTGTGGACAAGCCAGCTA+GGG - chr8.3:13448165-13448184 MS.gene61473:intron 60.0%
GTCTGCCACAGCGTCACCAT+AGG - chr8.3:13448949-13448968 MS.gene61473:intron 60.0%
TCCCACCTTGCCAAGTGCAG+TGG - chr8.3:13450036-13450055 MS.gene61473:CDS 60.0%
! AGGGAGATGTCCCTTTGGGG+AGG - chr8.3:13448231-13448250 MS.gene61473:intron 60.0%
GGGCTGTGGACAAGCCAGCT+AGG - chr8.3:13448164-13448183 MS.gene61473:intron 65.0%
TGGGGATGGCACTAGGGCTG+TGG - chr8.3:13448150-13448169 MS.gene61473:intron 65.0%
TGCTGCTGTCCGTCGCTGCG+AGG + chr8.3:13447932-13447951 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 13447557 13450066 13447557 ID=MS.gene61473
chr8.3 mRNA 13447557 13450066 13447557 ID=MS.gene61473.t1;Parent=MS.gene61473
chr8.3 exon 13449343 13450066 13449343 ID=MS.gene61473.t1.exon1;Parent=MS.gene61473.t1
chr8.3 CDS 13449343 13450066 13449343 ID=cds.MS.gene61473.t1;Parent=MS.gene61473.t1
chr8.3 exon 13447557 13447798 13447557 ID=MS.gene61473.t1.exon2;Parent=MS.gene61473.t1
chr8.3 CDS 13447557 13447798 13447557 ID=cds.MS.gene61473.t1;Parent=MS.gene61473.t1
Gene Sequence

>MS.gene61473

ATGGTTCTTCCAGACAATATTCCAACCTGTAATATGTCACCGCCGCAATTCACTACATCAAACAGCGATATCATTGAAGTGAGACCTCGATTTCCAATGAAAAATGAAAACTTGGAACAACATTCAACATTGTATCAAACCCATTCCTTCAAAAGGGCAAGAATTTCTGATAACAATCGTTCCAATGCTTTGATATGCCCTCCTCCAAAGATGGTTCTTCCTCCATCAAACAGAGCCGACCACGTTTTCTACAAGACTCGTATCTGTAATAAGTCCAGACTTGGTACTTGTAGAAACGGTAAAGATTGCAACTTTGCTCATGGGGTTGAAGAACTAAGACTGCCACCACGAAATTGGCAGGAGCTAGTTAGTCCTCGCAGCGACGGACAGCAGCAGCTGAGAAATTGGGAGGAAGATCAAAAAATGATTCACAAGATGAAGCTATGCAGGATGTATTCCAATGGTGAGAAATGTTTCTTTGGTAGTAAGTGTTATTTTCTTCATGAGGATCCTGCTAAGTTTAGGGATCATTCTTGGAAGTCAAGAGAGTGTTCTTCTATAAGCATTGGGGCTATTGGTTCTTCAAAGTCATTTGGGGATGGCACTAGGGCTGTGGACAAGCCAGCTAGGGGCACTTATTGGAAGACTAAGACGTGTTTCCATTGGCAGCATCGAGGGAGATGTCCCTTTGGGGAGGACTGTCAATTTGCTCATGGAGAAGCAGGTAATAATAACTCATCTTTGTGTTGATTTCCCTTTTCCCTATTTTGATTATTGATTTTGGTTTTATCATATAATTCTGCTGTACTGGTTTTGTTTTGTATAGAAAATATGTTTGTTGATTTTCTTGATGATGTGCAGAAGATCATTTTATGTTATATGATGTTCAAATTATAGTGGTTGAATGTATGTTATTTTCATTGCTGCGTATGATATTTTCATGTTTGGTATGATATTTTGTTCACACAACAAATGCTAATTCAGTTTAAAACTGTGTTGTCAAATATCGGCGGTCAAATGCCAGACTTTTAGGAAGCACCATGGCCAATTTGTGATGGATGGCGATGCCATGGCATTTTATGGTGGCTTAAAATTGCACATTTGGTTTTCCACCATCTGTCATCGTAAACACTGGTTTAAAATTAATTTACCACTAGTGATTATTTTATCTTTGTGTTCAAATTTCCTTGGTAGCAACGCATTGATATGTGAGATTTTGTGACAACTGCTATGTTTATATGGCGGATTTCAGTTGGCCGTCGTGATCCACCATCTGCTACTGATAACACTCCTTTATCTAAGGTTTACATATCAGTGTTGTTAAATGGCAGTGCCAAGACAGAATAGTGTGGCAGATTTTTGACATCAGCCATAAAAAATTTGTAAATGGATGTCTGCCACAGCGTCACCATAGGTCACAATGGTGTGTTTATATGTTGTATTTTTGGCCTTCCAGTATATTTAACCATCCTCCATGGACAACACTGTCACTGTGTTACATATAACTTGAATGAAGTTCTGTATTTTCGTGTCAGCCACATTAGGAATTTCTATTTGATAGAAATGTCTAGATTTCATAAATTAAAATAGTTAATCATCTCAATTTTATAAGGCATGATTACTATTAAATGGTGTTAAAAGTTTGTGTTTGTTACTTGACTGAAATTTTAGTAGTTTGGAGTAGTCTGCGAAAAATTTCCTTTCTGTTATCAGCTATAAAGCATGGGTACCCGTGGTGGTTACGCAAGTAGGTATCGAAATTGCTTGTCGGATAAAAAAAAAAAATTAGGATGCTGCACTTTGGTATATGTCGATGGATATTTTGGGTATGCATCCGTGATCCAATACATAGAAATACATGTGATTTTGAACAATAAACCATTATGTTATATTCATCTTTTCCAAACCTGTTTTTCCTTTTGGTTTCTGCTGATGGGTGTGCCTATAATTTGATGTCTAACTCATCAATAGTTTTGAGGTACATTGCTCATTTGCAAGGTTTTAAACTGCGACCATGTCACAATCCTTAATATTGCAGTCAAATATGGTTAATGCAGCTGCAGTTGCAGTGTCGGAGACTTAAAAAACCTCAATTAAAAAACCTCAACGTCTCTGCAACAATTGTGTTCACTGTCCATTTTTTATATATATTGCTTGTTTGCGTGTGATTACTTGTATTTACATATTGTGAACAAAAAGTACAAGATGATTTTACAATTTAAACTGTTTGGTTTCCATGTATCATACTCTGACTCATTGAAGTTGCAGTTGCAATAGCAAATTCAACGAAAGCTATCATTCCAACTTCACGAAAAGCCTTAACAGGTTCTGCCCATGATGCATATGCACCTACCACCTTCCCTGCAAGACTTATGGCAAAGGAAGAGGAGCAGGCTAAGAAGATGCATTTACTTTGGTTGAAATTAAACAAGATCAATCGTGTTTATGGTGATTGGATTGATGATTCACCTCTTGTTCTTCCCACCTTGCCAAGTGCAGTGGAGAACTGA
Protein sequence

>MS.gene61473.t1

MVLPDNIPTCNMSPPQFTTSNSDIIEVRPRFPMKNENLEQHSTLYQTHSFKRARISDNNRSNALICPPPKMVLPPSNRADHVFYKTRICNKSRLGTCRNGKDCNFAHGVEELRLPPRNWQELVSPRSDGQQQLRNWEEDQKMIHKMKLCRMYSNGEKCFFGSKCYFLHEDPAKFRDHSWKSRECSSISIGAIGSSKSFGDGTRAVDKPARGTYWKTKTCFHWQHRGRCPFGEDCQFAHGEAVAIANSTKAIIPTSRKALTGSAHDAYAPTTFPARLMAKEEEQAKKMHLLWLKLNKINRVYGDWIDDSPLVLPTLPSAVEN