Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61478.t1 | RHN63911.1 | 85.7 | 91 | 13 | 0 | 1 | 91 | 118 | 208 | 2.60E-39 | 171 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61478.t1 | Q9LT81 | 41.4 | 111 | 44 | 2 | 1 | 90 | 186 | 296 | 1.9e-14 | 79.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61478.t1 | G7JVB8 | 85.7 | 91 | 13 | 0 | 1 | 91 | 118 | 208 | 1.9e-39 | 171.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene61478.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61478.t1 | MTR_4g113940 | 85.714 | 91 | 13 | 0 | 1 | 91 | 118 | 208 | 5.66e-53 | 166 |
| MS.gene61478.t1 | MTR_4g113970 | 60.000 | 105 | 30 | 2 | 1 | 94 | 147 | 250 | 2.51e-33 | 115 |
| MS.gene61478.t1 | MTR_4g113890 | 54.945 | 91 | 11 | 1 | 1 | 91 | 116 | 176 | 3.30e-25 | 94.4 |
| MS.gene61478.t1 | MTR_4g113860 | 50.526 | 95 | 42 | 1 | 1 | 90 | 146 | 240 | 3.59e-23 | 90.5 |
| MS.gene61478.t1 | MTR_3g050390 | 49.474 | 95 | 30 | 3 | 1 | 90 | 95 | 176 | 3.31e-19 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61478.t1 | AT3G19360 | 40.541 | 111 | 45 | 2 | 1 | 90 | 186 | 296 | 6.73e-19 | 79.7 |
| MS.gene61478.t1 | AT3G19360 | 40.541 | 111 | 45 | 2 | 1 | 90 | 186 | 296 | 6.73e-19 | 79.7 |
Find 25 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCGTTGGAACTAATAATT+TGG | 0.191122 | 8.3:-13417339 | MS.gene61478:CDS |
| ATGAAGATCCTGCACAGTTT+AGG | 0.265678 | 8.3:-13417397 | MS.gene61478:CDS |
| TGAAGATCCTGCACAGTTTA+GGG | 0.304032 | 8.3:-13417396 | MS.gene61478:CDS |
| AATTTACAGTTATCTCTATA+TGG | 0.334708 | 8.3:+13417425 | None:intergenic |
| CATACCGGATATTGTTCCTT+TGG | 0.350886 | 8.3:-13417245 | MS.gene61478:CDS |
| GGAGTACTCTCTTGATTTCA+AGG | 0.405462 | 8.3:+13417367 | None:intergenic |
| AGACTAAGCTGTGTCGCAAT+TGG | 0.427484 | 8.3:-13417271 | MS.gene61478:CDS |
| AGCAAGCAAGGGGCACTTAT+CGG | 0.435970 | 8.3:-13417295 | MS.gene61478:CDS |
| CTAATAATTTGGAAGGCAGT+AGG | 0.449439 | 8.3:-13417328 | MS.gene61478:CDS |
| CATTCCAAAGGAACAATATC+CGG | 0.460353 | 8.3:+13417241 | None:intergenic |
| TGTGCAATAAGTATTGCAAT+GGG | 0.484478 | 8.3:-13417457 | MS.gene61478:CDS |
| CTGTGCAATAAGTATTGCAA+TGG | 0.513646 | 8.3:-13417458 | MS.gene61478:CDS |
| GGGCTGTGAACAAGCAAGCA+AGG | 0.529652 | 8.3:-13417307 | MS.gene61478:CDS |
| TAATAATTTGGAAGGCAGTA+GGG | 0.531942 | 8.3:-13417327 | MS.gene61478:CDS |
| GAATCATCCCTAAACTGTGC+AGG | 0.539981 | 8.3:+13417389 | None:intergenic |
| GTTGGAACTAATAATTTGGA+AGG | 0.547263 | 8.3:-13417335 | MS.gene61478:CDS |
| GGCTGTGAACAAGCAAGCAA+GGG | 0.555542 | 8.3:-13417306 | MS.gene61478:CDS |
| TGTCGCAATTGGAAGCATAC+CGG | 0.563682 | 8.3:-13417260 | MS.gene61478:CDS |
| ATTAGTTCCAACGATTATTG+CGG | 0.587078 | 8.3:+13417346 | None:intergenic |
| ACTGTGCAGGATCTTCATGA+AGG | 0.599205 | 8.3:+13417402 | None:intergenic |
| AAAGAGACAGTTCATTCCAA+AGG | 0.613697 | 8.3:+13417229 | None:intergenic |
| GAGTACTCCGCAATAATCGT+TGG | 0.615437 | 8.3:-13417353 | MS.gene61478:CDS |
| CAATAAGTATTGCAATGGGG+AGG | 0.655957 | 8.3:-13417453 | MS.gene61478:CDS |
| GCTGTGAACAAGCAAGCAAG+GGG | 0.667598 | 8.3:-13417305 | MS.gene61478:CDS |
| GTGCAATAAGTATTGCAATG+GGG | 0.763140 | 8.3:-13417456 | MS.gene61478:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTACAGTTATCTCTATA+TGG | + | chr8.3:13417144-13417163 | None:intergenic | 20.0% |
| !!! | CAATAACTCATTTTGATTAT+TGG | - | chr8.3:13417380-13417399 | MS.gene61478:CDS | 20.0% |
| ! | AATCGTTGGAACTAATAATT+TGG | - | chr8.3:13417227-13417246 | MS.gene61478:CDS | 25.0% |
| !!! | CTCATTTTGATTATTGGTTT+TGG | - | chr8.3:13417386-13417405 | MS.gene61478:CDS | 25.0% |
| ATTAGTTCCAACGATTATTG+CGG | + | chr8.3:13417223-13417242 | None:intergenic | 30.0% | |
| GTTGGAACTAATAATTTGGA+AGG | - | chr8.3:13417231-13417250 | MS.gene61478:CDS | 30.0% | |
| TAATAATTTGGAAGGCAGTA+GGG | - | chr8.3:13417239-13417258 | MS.gene61478:CDS | 30.0% | |
| ! | TGTGCAATAAGTATTGCAAT+GGG | - | chr8.3:13417109-13417128 | MS.gene61478:intron | 30.0% |
| AAAGAGACAGTTCATTCCAA+AGG | + | chr8.3:13417340-13417359 | None:intergenic | 35.0% | |
| CATTCCAAAGGAACAATATC+CGG | + | chr8.3:13417328-13417347 | None:intergenic | 35.0% | |
| CTAATAATTTGGAAGGCAGT+AGG | - | chr8.3:13417238-13417257 | MS.gene61478:CDS | 35.0% | |
| ! | CTGTGCAATAAGTATTGCAA+TGG | - | chr8.3:13417108-13417127 | MS.gene61478:intron | 35.0% |
| ! | GTGCAATAAGTATTGCAATG+GGG | - | chr8.3:13417110-13417129 | MS.gene61478:intron | 35.0% |
| ATGAAGATCCTGCACAGTTT+AGG | - | chr8.3:13417169-13417188 | MS.gene61478:intron | 40.0% | |
| CATACCGGATATTGTTCCTT+TGG | - | chr8.3:13417321-13417340 | MS.gene61478:CDS | 40.0% | |
| TGAAGATCCTGCACAGTTTA+GGG | - | chr8.3:13417170-13417189 | MS.gene61478:intron | 40.0% | |
| ! | CAATAAGTATTGCAATGGGG+AGG | - | chr8.3:13417113-13417132 | MS.gene61478:intron | 40.0% |
| ! | GGAGTACTCTCTTGATTTCA+AGG | + | chr8.3:13417202-13417221 | None:intergenic | 40.0% |
| ACTGTGCAGGATCTTCATGA+AGG | + | chr8.3:13417167-13417186 | None:intergenic | 45.0% | |
| AGACTAAGCTGTGTCGCAAT+TGG | - | chr8.3:13417295-13417314 | MS.gene61478:CDS | 45.0% | |
| GAATCATCCCTAAACTGTGC+AGG | + | chr8.3:13417180-13417199 | None:intergenic | 45.0% | |
| GAGTACTCCGCAATAATCGT+TGG | - | chr8.3:13417213-13417232 | MS.gene61478:CDS | 45.0% | |
| ! | TGTCGCAATTGGAAGCATAC+CGG | - | chr8.3:13417306-13417325 | MS.gene61478:CDS | 45.0% |
| GCTGTGAACAAGCAAGCAAG+GGG | - | chr8.3:13417261-13417280 | MS.gene61478:CDS | 50.0% | |
| GGCTGTGAACAAGCAAGCAA+GGG | - | chr8.3:13417260-13417279 | MS.gene61478:CDS | 50.0% | |
| !! | AGCAAGCAAGGGGCACTTAT+CGG | - | chr8.3:13417271-13417290 | MS.gene61478:CDS | 50.0% |
| GGGCTGTGAACAAGCAAGCA+AGG | - | chr8.3:13417259-13417278 | MS.gene61478:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 13417102 | 13417486 | 13417102 | ID=MS.gene61478 |
| chr8.3 | mRNA | 13417102 | 13417486 | 13417102 | ID=MS.gene61478.t1;Parent=MS.gene61478 |
| chr8.3 | exon | 13417213 | 13417486 | 13417213 | ID=MS.gene61478.t1.exon1;Parent=MS.gene61478.t1 |
| chr8.3 | CDS | 13417213 | 13417486 | 13417213 | ID=cds.MS.gene61478.t1;Parent=MS.gene61478.t1 |
| chr8.3 | exon | 13417102 | 13417112 | 13417102 | ID=MS.gene61478.t1.exon2;Parent=MS.gene61478.t1 |
| chr8.3 | CDS | 13417102 | 13417112 | 13417102 | ID=cds.MS.gene61478.t1;Parent=MS.gene61478.t1 |
| Gene Sequence |
| Protein sequence |
