Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61514.t1 | XP_013441754.1 | 86.7 | 294 | 38 | 1 | 1 | 293 | 1 | 294 | 1.40E-143 | 519.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61514.t1 | Q9LKR4 | 22.7 | 260 | 171 | 6 | 40 | 287 | 60 | 301 | 1.3e-12 | 75.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61514.t1 | A0A072TE13 | 86.7 | 294 | 38 | 1 | 1 | 293 | 1 | 294 | 9.9e-144 | 519.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene61514.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61514.t1 | MTR_0558s0020 | 86.441 | 295 | 39 | 1 | 1 | 294 | 1 | 295 | 0.0 | 539 |
| MS.gene61514.t1 | MTR_1g083640 | 82.042 | 284 | 35 | 1 | 1 | 268 | 1 | 284 | 3.12e-176 | 489 |
| MS.gene61514.t1 | MTR_8g446020 | 72.789 | 294 | 41 | 1 | 1 | 294 | 1 | 255 | 1.25e-155 | 436 |
| MS.gene61514.t1 | MTR_2g014100 | 81.633 | 245 | 36 | 1 | 1 | 236 | 1 | 245 | 8.93e-148 | 416 |
| MS.gene61514.t1 | MTR_6g090505 | 84.348 | 230 | 36 | 0 | 65 | 294 | 1 | 230 | 4.93e-146 | 416 |
| MS.gene61514.t1 | MTR_0337s0040 | 71.815 | 259 | 33 | 2 | 1 | 258 | 1 | 220 | 3.68e-128 | 365 |
| MS.gene61514.t1 | MTR_3g008240 | 84.236 | 203 | 31 | 1 | 1 | 202 | 1 | 203 | 3.93e-123 | 352 |
| MS.gene61514.t1 | MTR_4g040430 | 83.168 | 202 | 30 | 1 | 1 | 202 | 1 | 198 | 8.96e-122 | 348 |
| MS.gene61514.t1 | MTR_5g071590 | 73.333 | 180 | 35 | 2 | 1 | 167 | 1 | 180 | 6.98e-94 | 277 |
| MS.gene61514.t1 | MTR_1g055240 | 86.620 | 142 | 19 | 0 | 91 | 232 | 3 | 144 | 1.37e-89 | 265 |
| MS.gene61514.t1 | MTR_0019s0290 | 80.921 | 152 | 29 | 0 | 82 | 233 | 9 | 160 | 1.43e-88 | 263 |
| MS.gene61514.t1 | MTR_1281s0010 | 79.817 | 109 | 22 | 0 | 19 | 127 | 1 | 109 | 2.04e-59 | 186 |
| MS.gene61514.t1 | MTR_4g008820 | 83.750 | 80 | 12 | 1 | 1 | 79 | 125 | 204 | 3.79e-34 | 132 |
| MS.gene61514.t1 | MTR_8g046060 | 77.273 | 88 | 11 | 2 | 1 | 79 | 91 | 178 | 2.52e-33 | 122 |
| MS.gene61514.t1 | MTR_6g051425 | 31.325 | 249 | 156 | 4 | 40 | 288 | 22 | 255 | 2.96e-31 | 120 |
| MS.gene61514.t1 | MTR_1g070355 | 28.287 | 251 | 161 | 4 | 41 | 291 | 80 | 311 | 3.48e-20 | 91.7 |
| MS.gene61514.t1 | MTR_4g035820 | 22.654 | 309 | 205 | 8 | 17 | 317 | 61 | 343 | 2.28e-15 | 77.4 |
| MS.gene61514.t1 | MTR_0402s0040 | 25.405 | 185 | 119 | 4 | 49 | 233 | 40 | 205 | 4.37e-15 | 76.3 |
| MS.gene61514.t1 | MTR_0337s0050 | 67.500 | 40 | 13 | 0 | 259 | 298 | 14 | 53 | 1.51e-14 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61514.t1 | AT4G38180 | 28.788 | 264 | 171 | 4 | 32 | 295 | 70 | 316 | 8.71e-27 | 111 |
| MS.gene61514.t1 | AT5G28530 | 23.372 | 261 | 168 | 7 | 40 | 287 | 60 | 301 | 1.02e-14 | 75.5 |
| MS.gene61514.t1 | AT5G28530 | 23.372 | 261 | 168 | 7 | 40 | 287 | 60 | 301 | 1.02e-14 | 75.5 |
| MS.gene61514.t1 | AT5G28530 | 23.372 | 261 | 168 | 7 | 40 | 287 | 60 | 301 | 1.04e-14 | 75.5 |
| MS.gene61514.t1 | AT1G10240 | 24.609 | 256 | 163 | 9 | 49 | 296 | 62 | 295 | 4.00e-14 | 73.6 |
| MS.gene61514.t1 | AT1G10240 | 24.609 | 256 | 163 | 9 | 49 | 296 | 62 | 295 | 4.00e-14 | 73.6 |
| MS.gene61514.t1 | AT2G27110 | 26.901 | 171 | 108 | 4 | 127 | 295 | 108 | 263 | 4.14e-12 | 67.8 |
| MS.gene61514.t1 | AT2G27110 | 26.901 | 171 | 108 | 4 | 127 | 295 | 108 | 263 | 4.14e-12 | 67.8 |
| MS.gene61514.t1 | AT1G76320 | 21.908 | 283 | 189 | 6 | 41 | 321 | 3 | 255 | 7.19e-12 | 66.6 |
| MS.gene61514.t1 | AT1G76320 | 21.908 | 283 | 189 | 6 | 41 | 321 | 3 | 255 | 7.87e-12 | 66.6 |
| MS.gene61514.t1 | AT1G76320 | 21.908 | 283 | 189 | 6 | 41 | 321 | 3 | 255 | 8.24e-12 | 66.6 |
| MS.gene61514.t1 | AT1G76320 | 21.908 | 283 | 189 | 6 | 41 | 321 | 3 | 255 | 8.24e-12 | 66.6 |
| MS.gene61514.t1 | AT4G15090 | 25.403 | 248 | 164 | 6 | 50 | 296 | 66 | 293 | 8.54e-12 | 66.6 |
| MS.gene61514.t1 | AT4G15090 | 25.403 | 248 | 164 | 6 | 50 | 296 | 66 | 293 | 8.54e-12 | 66.6 |
| MS.gene61514.t1 | AT4G15090 | 25.403 | 248 | 164 | 6 | 50 | 296 | 66 | 293 | 8.54e-12 | 66.6 |
| MS.gene61514.t1 | AT4G15090 | 25.403 | 248 | 164 | 6 | 50 | 296 | 66 | 293 | 8.54e-12 | 66.6 |
| MS.gene61514.t1 | AT4G15090 | 25.403 | 248 | 164 | 6 | 50 | 296 | 66 | 293 | 8.54e-12 | 66.6 |
| MS.gene61514.t1 | AT2G27110 | 35.366 | 82 | 53 | 0 | 213 | 294 | 36 | 117 | 2.82e-11 | 65.1 |
Find 45 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACAAGATTATGCTCTATT+TGG | 0.198611 | 8.3:+13094025 | MS.gene61514:CDS |
| TATCGGCCGTTTACAGTAAC+TGG | 0.299041 | 8.3:+13094091 | MS.gene61514:CDS |
| CCTTATTCTGAAAATTTGTA+TGG | 0.314405 | 8.3:+13094175 | MS.gene61514:CDS |
| GCCGATATTTATTGTTTGTT+CGG | 0.361178 | 8.3:-13094075 | None:intergenic |
| TTCTCATACTCAACAAGAAC+TGG | 0.395633 | 8.3:-13093545 | None:intergenic |
| CCATGATTCTTTATCACTAA+AGG | 0.406174 | 8.3:-13093306 | None:intergenic |
| ACCGAACAAACAATAAATAT+CGG | 0.406375 | 8.3:+13094074 | MS.gene61514:CDS |
| TAAAGAATCATGGAAGTCTT+TGG | 0.408500 | 8.3:+13093316 | MS.gene61514:CDS |
| TCGTTTCCAGTTACTGTAAA+CGG | 0.409191 | 8.3:-13094097 | None:intergenic |
| ATAACACTGGATTCTCTGTT+AGG | 0.417808 | 8.3:+13093387 | MS.gene61514:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 8.3:+13093722 | MS.gene61514:CDS |
| AGCTGGAATATATTTGAAGT+TGG | 0.433136 | 8.3:+13093604 | MS.gene61514:CDS |
| TGAATGTCTATAAGATATAT+AGG | 0.445219 | 8.3:+13093636 | MS.gene61514:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 8.3:+13093587 | MS.gene61514:CDS |
| CGTTATGCTTGTAACAAAGA+AGG | 0.449439 | 8.3:+13093470 | MS.gene61514:CDS |
| GCTTTAATAAATTCTAAAGC+TGG | 0.450264 | 8.3:+13093752 | MS.gene61514:CDS |
| CTCAAGTAGCGCAAATGCTC+TGG | 0.460440 | 8.3:-13093692 | None:intergenic |
| ACAAAATAAGAAATCGGATA+AGG | 0.469178 | 8.3:+13093436 | MS.gene61514:CDS |
| ATCCGAGAGTGGACAGTTGA+CGG | 0.481992 | 8.3:+13093241 | MS.gene61514:CDS |
| GAAGTGATGGGTCAAGGAGT+TGG | 0.484292 | 8.3:+13093797 | MS.gene61514:CDS |
| TTACAACCAACCCTTTCCTC+TGG | 0.485330 | 8.3:-13093581 | None:intergenic |
| TTTAATTATGCATATAACAC+TGG | 0.485542 | 8.3:+13093374 | MS.gene61514:CDS |
| TGTCTTCTGCAGCATCAACT+TGG | 0.487243 | 8.3:-13093970 | None:intergenic |
| CAATTCACAAAATAAGAAAT+CGG | 0.490263 | 8.3:+13093430 | MS.gene61514:CDS |
| GAGAACATCTTATGAAGTGA+TGG | 0.499875 | 8.3:+13093784 | MS.gene61514:CDS |
| TTTAAGTCCGAAAATCGAAA+AGG | 0.508080 | 8.3:-13093836 | None:intergenic |
| AACCGTCAACTGTCCACTCT+CGG | 0.519082 | 8.3:-13093243 | None:intergenic |
| TGTCTATAAGATATATAGGT+GGG | 0.523740 | 8.3:+13093640 | MS.gene61514:CDS |
| TAACAAAGAAGGTTTCAAGA+AGG | 0.538422 | 8.3:+13093481 | MS.gene61514:CDS |
| CGTCAAAAGAAGATGGTGGC+TGG | 0.540133 | 8.3:+13093878 | MS.gene61514:CDS |
| CCTTTAGTGATAAAGAATCA+TGG | 0.543522 | 8.3:+13093306 | MS.gene61514:CDS |
| AACAAAGAAGGTTTCAAGAA+GGG | 0.545537 | 8.3:+13093482 | MS.gene61514:CDS |
| CTTATTCTGAAAATTTGTAT+GGG | 0.551924 | 8.3:+13094176 | MS.gene61514:CDS |
| GACAACTCGTCAAAAGAAGA+TGG | 0.552238 | 8.3:+13093871 | MS.gene61514:CDS |
| AAATGCTCTGGTTTATGTAA+TGG | 0.552634 | 8.3:-13093680 | None:intergenic |
| AATGAAGCTCTCTCGAAACC+AGG | 0.565261 | 8.3:+13093932 | MS.gene61514:CDS |
| TGAAAATTCAGTTATTGATG+CGG | 0.565546 | 8.3:+13093265 | MS.gene61514:CDS |
| GTGATGGGTCAAGGAGTTGG+AGG | 0.568802 | 8.3:+13093800 | MS.gene61514:CDS |
| TCTTATGAAGTGATGGGTCA+AGG | 0.589029 | 8.3:+13093791 | MS.gene61514:CDS |
| AGAACATCTTATGAAGTGAT+GGG | 0.601988 | 8.3:+13093785 | MS.gene61514:CDS |
| ATATCAACTTCATCCGAGAG+TGG | 0.612216 | 8.3:+13093230 | MS.gene61514:CDS |
| AGAACTGGACTGTCACATGT+TGG | 0.620791 | 8.3:-13093530 | None:intergenic |
| GGGTCAAGGAGTTGGAGGCA+CGG | 0.621882 | 8.3:+13093805 | MS.gene61514:CDS |
| ATGTCTATAAGATATATAGG+TGG | 0.643489 | 8.3:+13093639 | MS.gene61514:CDS |
| AACTCGTCAAAAGAAGATGG+TGG | 0.679247 | 8.3:+13093874 | MS.gene61514:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAATTCACAAAATAAGAAAT+CGG | + | chr8.3:13093430-13093449 | MS.gene61514:CDS | 20.0% |
| !! | TGAATGTCTATAAGATATAT+AGG | + | chr8.3:13093636-13093655 | MS.gene61514:CDS | 20.0% |
| !! | TTTAATTATGCATATAACAC+TGG | + | chr8.3:13093374-13093393 | MS.gene61514:CDS | 20.0% |
| !!! | AAGACATAACTAGTATTTTT+TGG | + | chr8.3:13093987-13094006 | MS.gene61514:CDS | 20.0% |
| !!! | AGACATAACTAGTATTTTTT+GGG | + | chr8.3:13093988-13094007 | MS.gene61514:CDS | 20.0% |
| !!! | CTTATTCTGAAAATTTGTAT+GGG | + | chr8.3:13094176-13094195 | MS.gene61514:CDS | 20.0% |
| ! | ACAAAATAAGAAATCGGATA+AGG | + | chr8.3:13093436-13093455 | MS.gene61514:CDS | 25.0% |
| ! | ACCGAACAAACAATAAATAT+CGG | + | chr8.3:13094074-13094093 | MS.gene61514:CDS | 25.0% |
| ! | ATGTCTATAAGATATATAGG+TGG | + | chr8.3:13093639-13093658 | MS.gene61514:CDS | 25.0% |
| ! | TGAAAATTCAGTTATTGATG+CGG | + | chr8.3:13093265-13093284 | MS.gene61514:CDS | 25.0% |
| ! | TGTCTATAAGATATATAGGT+GGG | + | chr8.3:13093640-13093659 | MS.gene61514:CDS | 25.0% |
| !! | CCATACAAATTTTCAGAATA+AGG | - | chr8.3:13094178-13094197 | None:intergenic | 25.0% |
| !! | CCTTATTCTGAAAATTTGTA+TGG | + | chr8.3:13094175-13094194 | MS.gene61514:CDS | 25.0% |
| !! | GAATTTTCCTTTTCGATTTT+CGG | + | chr8.3:13093829-13093848 | MS.gene61514:CDS | 25.0% |
| !!! | CTTATTTTGTGAATTGTTCT+TGG | - | chr8.3:13093426-13093445 | None:intergenic | 25.0% |
| !!! | GCTTTAATAAATTCTAAAGC+TGG | + | chr8.3:13093752-13093771 | MS.gene61514:CDS | 25.0% |
| AACAAAGAAGGTTTCAAGAA+GGG | + | chr8.3:13093482-13093501 | MS.gene61514:CDS | 30.0% | |
| AGAACATCTTATGAAGTGAT+GGG | + | chr8.3:13093785-13093804 | MS.gene61514:CDS | 30.0% | |
| CAACAAGATTATGCTCTATT+TGG | + | chr8.3:13094025-13094044 | MS.gene61514:CDS | 30.0% | |
| CCATGATTCTTTATCACTAA+AGG | - | chr8.3:13093309-13093328 | None:intergenic | 30.0% | |
| CCTTTAGTGATAAAGAATCA+TGG | + | chr8.3:13093306-13093325 | MS.gene61514:CDS | 30.0% | |
| GCCGATATTTATTGTTTGTT+CGG | - | chr8.3:13094078-13094097 | None:intergenic | 30.0% | |
| TAAAGAAAAACCAGAGGAAA+GGG | + | chr8.3:13093571-13093590 | MS.gene61514:CDS | 30.0% | |
| TAAAGAATCATGGAAGTCTT+TGG | + | chr8.3:13093316-13093335 | MS.gene61514:CDS | 30.0% | |
| TAACAAAGAAGGTTTCAAGA+AGG | + | chr8.3:13093481-13093500 | MS.gene61514:CDS | 30.0% | |
| TGGATATCATAGTAAAAACC+TGG | - | chr8.3:13093953-13093972 | None:intergenic | 30.0% | |
| TTTAAGTCCGAAAATCGAAA+AGG | - | chr8.3:13093839-13093858 | None:intergenic | 30.0% | |
| ! | AAATGCTCTGGTTTATGTAA+TGG | - | chr8.3:13093683-13093702 | None:intergenic | 30.0% |
| ! | AGCTGGAATATATTTGAAGT+TGG | + | chr8.3:13093604-13093623 | MS.gene61514:CDS | 30.0% |
| !!! | ACGTCTGACATTTTTTGTTT+TGG | + | chr8.3:13094133-13094152 | MS.gene61514:CDS | 30.0% |
| ATAACACTGGATTCTCTGTT+AGG | + | chr8.3:13093387-13093406 | MS.gene61514:CDS | 35.0% | |
| CGAGAAGTAAATGAAGTTCA+AGG | + | chr8.3:13093722-13093741 | MS.gene61514:CDS | 35.0% | |
| CGTTATGCTTGTAACAAAGA+AGG | + | chr8.3:13093470-13093489 | MS.gene61514:CDS | 35.0% | |
| CTAAAGAAAAACCAGAGGAA+AGG | + | chr8.3:13093570-13093589 | MS.gene61514:CDS | 35.0% | |
| GAAAGCTAAAGAAAAACCAG+AGG | + | chr8.3:13093565-13093584 | MS.gene61514:CDS | 35.0% | |
| GAGAACATCTTATGAAGTGA+TGG | + | chr8.3:13093784-13093803 | MS.gene61514:CDS | 35.0% | |
| GTCTGCTTAATCCTAAAAAC+AGG | + | chr8.3:13093507-13093526 | MS.gene61514:CDS | 35.0% | |
| TCGTTTCCAGTTACTGTAAA+CGG | - | chr8.3:13094100-13094119 | None:intergenic | 35.0% | |
| TTCTCATACTCAACAAGAAC+TGG | - | chr8.3:13093548-13093567 | None:intergenic | 35.0% | |
| !!! | ACTAGTATTTTTTGGGCAGA+TGG | + | chr8.3:13093995-13094014 | MS.gene61514:CDS | 35.0% |
| AACTCGTCAAAAGAAGATGG+TGG | + | chr8.3:13093874-13093893 | MS.gene61514:CDS | 40.0% | |
| ATATCAACTTCATCCGAGAG+TGG | + | chr8.3:13093230-13093249 | MS.gene61514:CDS | 40.0% | |
| GACAACTCGTCAAAAGAAGA+TGG | + | chr8.3:13093871-13093890 | MS.gene61514:CDS | 40.0% | |
| !! | TCACATGTTGGCCTGTTTTT+AGG | - | chr8.3:13093521-13093540 | None:intergenic | 40.0% |
| !! | TCTTATGAAGTGATGGGTCA+AGG | + | chr8.3:13093791-13093810 | MS.gene61514:CDS | 40.0% |
| AATGAAGCTCTCTCGAAACC+AGG | + | chr8.3:13093932-13093951 | MS.gene61514:CDS | 45.0% | |
| AGAACTGGACTGTCACATGT+TGG | - | chr8.3:13093533-13093552 | None:intergenic | 45.0% | |
| GAAAAACCAGAGGAAAGGGT+TGG | + | chr8.3:13093575-13093594 | MS.gene61514:CDS | 45.0% | |
| TATCGGCCGTTTACAGTAAC+TGG | + | chr8.3:13094091-13094110 | MS.gene61514:CDS | 45.0% | |
| TGTCTTCTGCAGCATCAACT+TGG | - | chr8.3:13093973-13093992 | None:intergenic | 45.0% | |
| TTACAACCAACCCTTTCCTC+TGG | - | chr8.3:13093584-13093603 | None:intergenic | 45.0% | |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | + | chr8.3:13093587-13093606 | MS.gene61514:CDS | 45.0% |
| AACCGTCAACTGTCCACTCT+CGG | - | chr8.3:13093246-13093265 | None:intergenic | 50.0% | |
| ATCCGAGAGTGGACAGTTGA+CGG | + | chr8.3:13093241-13093260 | MS.gene61514:CDS | 50.0% | |
| CTCAAGTAGCGCAAATGCTC+TGG | - | chr8.3:13093695-13093714 | None:intergenic | 50.0% | |
| ! | CGTCAAAAGAAGATGGTGGC+TGG | + | chr8.3:13093878-13093897 | MS.gene61514:CDS | 50.0% |
| !! | GAAGTGATGGGTCAAGGAGT+TGG | + | chr8.3:13093797-13093816 | MS.gene61514:CDS | 50.0% |
| !! | GTGATGGGTCAAGGAGTTGG+AGG | + | chr8.3:13093800-13093819 | MS.gene61514:CDS | 55.0% |
| GGGTCAAGGAGTTGGAGGCA+CGG | + | chr8.3:13093805-13093824 | MS.gene61514:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 13093221 | 13094219 | 13093221 | ID=MS.gene61514 |
| chr8.3 | mRNA | 13093221 | 13094219 | 13093221 | ID=MS.gene61514.t1;Parent=MS.gene61514 |
| chr8.3 | exon | 13093221 | 13094219 | 13093221 | ID=MS.gene61514.t1.exon1;Parent=MS.gene61514.t1 |
| chr8.3 | CDS | 13093221 | 13094219 | 13093221 | ID=cds.MS.gene61514.t1;Parent=MS.gene61514.t1 |
| Gene Sequence |
| Protein sequence |
