AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene61654


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61654.t1 MTR_4g051538 89.787 235 23 1 1 235 1 234 1.34e-144 403
MS.gene61654.t1 MTR_3g104630 51.969 127 49 3 106 220 16 142 4.45e-31 112
MS.gene61654.t1 MTR_5g053390 28.634 227 111 8 1 205 1 198 6.30e-17 77.4
MS.gene61654.t1 MTR_1g105920 31.098 164 96 4 1 150 1 161 1.31e-16 76.6
MS.gene61654.t1 MTR_3g080940 44.706 85 46 1 1 85 1 84 2.67e-16 75.1
MS.gene61654.t1 MTR_1g108510 30.323 155 96 4 1 143 1 155 2.78e-16 75.5
MS.gene61654.t1 MTR_8g086290 31.410 156 99 2 4 151 7 162 4.95e-16 73.6
MS.gene61654.t1 MTR_3g030770 30.323 155 96 4 1 143 1 155 5.68e-16 74.7
MS.gene61654.t1 MTR_1g012570 28.866 194 115 7 1 182 1 183 7.38e-16 74.3
MS.gene61654.t1 MTR_1g105910 32.609 138 78 3 1 126 1 135 8.94e-16 74.3
MS.gene61654.t1 MTR_2g030740 33.333 135 81 3 1 126 1 135 1.33e-15 73.9
MS.gene61654.t1 MTR_1g077360 40.244 82 49 0 1 82 1 82 1.74e-15 73.6
MS.gene61654.t1 MTR_3g052920 32.653 147 89 2 1 137 1 147 1.85e-15 73.2
MS.gene61654.t1 MTR_8g043650 31.852 135 83 3 1 126 1 135 2.16e-15 73.2
MS.gene61654.t1 MTR_1g106070 31.707 164 101 5 1 153 1 164 2.61e-15 72.0
MS.gene61654.t1 MTR_3g052870 40.244 82 49 0 1 82 1 82 4.18e-15 72.0
MS.gene61654.t1 MTR_2g085250 29.710 138 88 3 1 129 1 138 4.94e-15 72.0
MS.gene61654.t1 MTR_1g108580 43.056 72 41 0 1 72 1 72 5.14e-15 72.0
MS.gene61654.t1 MTR_2g085280 29.710 138 88 3 1 129 1 138 5.30e-15 72.0
MS.gene61654.t1 MTR_2g105290 29.747 158 99 4 4 151 7 162 5.92e-15 70.9
MS.gene61654.t1 MTR_5g055100 26.786 168 103 4 1 156 1 160 1.06e-14 71.2
MS.gene61654.t1 MTR_5g045560 40.260 77 46 0 1 77 1 77 4.99e-14 69.3
MS.gene61654.t1 MTR_2g093190 29.747 158 93 5 1 143 1 155 5.56e-14 69.3
MS.gene61654.t1 MTR_1g075600 27.919 197 113 5 1 182 1 183 1.35e-13 68.2
MS.gene61654.t1 MTR_0121s0100 30.579 121 75 2 1 115 1 118 1.74e-13 65.9
MS.gene61654.t1 MTR_4g094638 39.130 69 42 0 9 77 2 70 1.92e-13 66.6
MS.gene61654.t1 MTR_4g094632 36.145 83 53 0 9 91 2 84 2.63e-13 65.9
MS.gene61654.t1 MTR_3g030780 37.500 72 45 0 1 72 1 72 1.08e-12 63.5
MS.gene61654.t1 MTR_0121s0080 25.904 166 106 4 1 152 1 163 2.71e-12 64.7
MS.gene61654.t1 MTR_8g046350 28.947 114 68 2 9 116 2 108 1.36e-11 61.2
MS.gene61654.t1 MTR_8g079502 33.750 80 52 1 9 87 2 81 7.28e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61654.t1 AT4G36590 50.000 68 34 0 5 72 3 70 1.68e-17 79.3
MS.gene61654.t1 AT1G29962 46.250 80 42 1 1 80 1 79 1.51e-16 75.5
MS.gene61654.t1 AT2G34440 35.659 129 75 4 9 130 2 129 4.04e-16 73.9
MS.gene61654.t1 AT2G24840 43.750 80 45 0 7 86 61 140 1.02e-15 74.7
MS.gene61654.t1 AT5G60440 28.293 205 119 6 5 200 3 188 2.15e-14 71.2
MS.gene61654.t1 AT1G28460 45.455 77 41 1 1 77 1 76 4.98e-14 68.6
MS.gene61654.t1 AT3G04100 37.931 87 51 1 2 88 7 90 1.34e-12 65.1
MS.gene61654.t1 AT1G01530 32.710 107 68 1 5 107 3 109 1.76e-12 65.5
MS.gene61654.t1 AT1G28450 41.250 80 46 1 1 80 1 79 8.68e-12 62.4
MS.gene61654.t1 AT1G65360 43.333 60 34 0 9 68 7 66 9.59e-12 63.2

Find 43 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCGAGTTAACAACACTTTC+AGG 0.165743 4.1:-46909264 MS.gene61654:CDS
TTGAAGAAGTTGGAAAGTTC+TGG 0.270990 4.1:-46909033 MS.gene61654:CDS
TGCACCAACTTCATGATCTT+TGG 0.281401 4.1:+46908704 None:intergenic
ACACAAGGAGTCAACCTTAT+TGG 0.301989 4.1:-46908321 MS.gene61654:CDS
GATGGGTTTCCATAACAAAA+TGG 0.311638 4.1:+46909201 None:intergenic
ATAACCAATGTCTTGAAAAC+TGG 0.328346 4.1:-46908931 MS.gene61654:CDS
ATCATTTCCTCTTCCATTGA+AGG 0.330256 4.1:+46909138 None:intergenic
CTTCTACCATGTCATATATC+TGG 0.335672 4.1:+46908255 None:intergenic
CTTATTGGTGTAAGTGGAAT+AGG 0.342391 4.1:-46908306 MS.gene61654:CDS
CTATTCCACTTACACCAATA+AGG 0.342684 4.1:+46908307 None:intergenic
TTTCTTTCACTTGATCATAT+TGG 0.353442 4.1:+46908989 None:intergenic
AGTTGGAAAGTTCTGGATAT+TGG 0.382288 4.1:-46909026 MS.gene61654:CDS
ATTCTCATGCTTTCTCCAAC+GGG 0.403336 4.1:-46909228 MS.gene61654:CDS
TTTATTTACGACAGCAACTA+AGG 0.422183 4.1:+46908957 None:intergenic
TTTGTTATAGATTCAGAAGA+TGG 0.427015 4.1:+46909183 None:intergenic
GGAAGAGGAAATGATGAAAC+CGG 0.442189 4.1:-46909130 MS.gene61654:CDS
CCGAGTTAACAACACTTTCA+GGG 0.450892 4.1:-46909263 MS.gene61654:CDS
CCCTGAAAGTGTTGTTAACT+CGG 0.457016 4.1:+46909263 None:intergenic
GGTGTAAGTGGAATAGGATA+TGG 0.462286 4.1:-46908300 MS.gene61654:CDS
GTCAACCTTATTGGTGTAAG+TGG 0.466219 4.1:-46908312 MS.gene61654:CDS
CGTAGCAATTTCTACCATTG+AGG 0.467584 4.1:-46908775 MS.gene61654:intron
GAAGGATGAAAATCCTTCAA+TGG 0.468568 4.1:-46909151 MS.gene61654:CDS
GACTCCATTCAGTTCCTCAA+TGG 0.472764 4.1:+46908761 None:intergenic
TGTTGCATTTACAACGAGAA+AGG 0.475609 4.1:-46909307 MS.gene61654:CDS
TATTCTCATGCTTTCTCCAA+CGG 0.502164 4.1:-46909229 MS.gene61654:CDS
TGAAAATCCTTCAATGGAAG+AGG 0.510231 4.1:-46909145 MS.gene61654:CDS
TTGTTATAGATTCAGAAGAT+GGG 0.510327 4.1:+46909184 None:intergenic
TCTACCATTGAGGAACTGAA+TGG 0.512244 4.1:-46908765 MS.gene61654:CDS
TTGTCCTCAAAGACAAAAGA+AGG 0.512656 4.1:+46908729 None:intergenic
GCATTTACAACGAGAAAGGC+TGG 0.523590 4.1:-46909303 MS.gene61654:CDS
TTATCCAAAAGGTAAAGGTA+TGG 0.531949 4.1:-46908379 MS.gene61654:intron
GCGCCGAAGAGGTCATCAAA+AGG 0.555723 4.1:-46909378 MS.gene61654:CDS
AACACCAAAGATCATGAAGT+TGG 0.566706 4.1:-46908708 MS.gene61654:CDS
TGGATATTGGTCGATAAAAG+AGG 0.572226 4.1:-46909013 MS.gene61654:CDS
CGAGTTAACAACACTTTCAG+GGG 0.590811 4.1:-46909262 MS.gene61654:CDS
ACCAATGTCTTGAAAACTGG+AGG 0.591573 4.1:-46908928 MS.gene61654:CDS
GAGTAAGAATGGCGCCGAAG+AGG 0.609055 4.1:-46909389 None:intergenic
AGTGGAATAGGATATGGAAG+TGG 0.614001 4.1:-46908294 MS.gene61654:CDS
GGAGGAGAGATTGACCCTCA+TGG 0.634950 4.1:-46908910 MS.gene61654:intron
AAGATGTACACATACCATGA+GGG 0.689701 4.1:+46908896 None:intergenic
TGAAAGTGTTGTTAACTCGG+AGG 0.701093 4.1:+46909266 None:intergenic
AAGCATGAGAATATCAACTG+AGG 0.718686 4.1:+46909239 None:intergenic
AAAGGCTGGAATTTACAACA+AGG 0.729615 4.1:-46909289 MS.gene61654:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAATTGAATCAATGAA+AGG + chr4.1:46909249-46909268 None:intergenic 15.0%
!! CAATACATAATATATATGTT+AGG + chr4.1:46909221-46909240 None:intergenic 15.0%
!! CTTAAAATAATTAGGTAAAA+AGG + chr4.1:46908832-46908851 None:intergenic 15.0%
!!! TTCAATTTTTTTTATCCAAA+AGG - chr4.1:46909257-46909276 MS.gene61654:CDS 15.0%
!! CAGATTCACTTAAAATAATT+AGG + chr4.1:46908840-46908859 None:intergenic 20.0%
!! GTTGAAAAAAATTGAGAAAA+AGG - chr4.1:46908307-46908326 MS.gene61654:CDS 20.0%
!!! CTAATTATTTTAAGTGAATC+TGG - chr4.1:46908838-46908857 MS.gene61654:intron 20.0%
!!! TATAACAAAAACTAGTTTGA+AGG - chr4.1:46908478-46908497 MS.gene61654:intron 20.0%
!!! TGAAAAAAATGATGATCTTT+TGG - chr4.1:46908556-46908575 MS.gene61654:intron 20.0%
!!! TTTTTTTATCCAAAAGGTAA+AGG - chr4.1:46909263-46909282 MS.gene61654:CDS 20.0%
! ACGTAAAAATTTGAAGAAGT+TGG - chr4.1:46908604-46908623 MS.gene61654:intron 25.0%
! ATAGCTACAAAAAAAACACA+AGG - chr4.1:46909311-46909330 MS.gene61654:CDS 25.0%
! TCTTCTTAATTTCCATTACT+AGG - chr4.1:46909158-46909177 MS.gene61654:CDS 25.0%
! TTGTTATAGATTCAGAAGAT+GGG + chr4.1:46908466-46908485 None:intergenic 25.0%
! TTTCTTTCACTTGATCATAT+TGG + chr4.1:46908661-46908680 None:intergenic 25.0%
!! TTTGTTATAGATTCAGAAGA+TGG + chr4.1:46908467-46908486 None:intergenic 25.0%
!! TTTTTCATGAAATATCCTAG+CGG - chr4.1:46909005-46909024 MS.gene61654:CDS 25.0%
AATGACGTCAAATGATTTCA+TGG - chr4.1:46908803-46908822 MS.gene61654:intron 30.0%
ATAACCAATGTCTTGAAAAC+TGG - chr4.1:46908716-46908735 MS.gene61654:CDS 30.0%
CTAGAGATATTTGATGTGTT+GGG + chr4.1:46909086-46909105 None:intergenic 30.0%
TCTAGAGATATTTGATGTGT+TGG + chr4.1:46909087-46909106 None:intergenic 30.0%
TTATCCAAAAGGTAAAGGTA+TGG - chr4.1:46909268-46909287 MS.gene61654:CDS 30.0%
TTTATTTACGACAGCAACTA+AGG + chr4.1:46908693-46908712 None:intergenic 30.0%
! AAATCCTTCTTTTGTCTTTG+AGG - chr4.1:46908914-46908933 MS.gene61654:CDS 30.0%
! TCATTTTGCTCTATAATCTC+CGG + chr4.1:46908539-46908558 None:intergenic 30.0%
! TTTGGATAAAAAGCATGTTG+CGG - chr4.1:46908574-46908593 MS.gene61654:intron 30.0%
! TTTTGTAGCTATATCTTGAG+AGG + chr4.1:46909303-46909322 None:intergenic 30.0%
!! ACCATTTAAGTTGCACTTAT+GGG - chr4.1:46909117-46909136 MS.gene61654:CDS 30.0%
!! TACCATTTAAGTTGCACTTA+TGG - chr4.1:46909116-46909135 MS.gene61654:CDS 30.0%
AAAGGCTGGAATTTACAACA+AGG - chr4.1:46908358-46908377 MS.gene61654:CDS 35.0%
AACACCAAAGATCATGAAGT+TGG - chr4.1:46908939-46908958 MS.gene61654:CDS 35.0%
AAGATGTACACATACCATGA+GGG + chr4.1:46908754-46908773 None:intergenic 35.0%
AAGCATGAGAATATCAACTG+AGG + chr4.1:46908411-46908430 None:intergenic 35.0%
AATGTTTGCATGCCTAGTAA+TGG + chr4.1:46909173-46909192 None:intergenic 35.0%
ATCATTTCCTCTTCCATTGA+AGG + chr4.1:46908512-46908531 None:intergenic 35.0%
CTATTCCACTTACACCAATA+AGG + chr4.1:46909343-46909362 None:intergenic 35.0%
CTTATTGGTGTAAGTGGAAT+AGG - chr4.1:46909341-46909360 MS.gene61654:CDS 35.0%
GAAGGATGAAAATCCTTCAA+TGG - chr4.1:46908496-46908515 MS.gene61654:intron 35.0%
TAGACAAGATCAGATGTTGT+TGG - chr4.1:46909050-46909069 MS.gene61654:CDS 35.0%
TATTCTCATGCTTTCTCCAA+CGG - chr4.1:46908418-46908437 MS.gene61654:intron 35.0%
TCCCATAAGTGCAACTTAAA+TGG + chr4.1:46909121-46909140 None:intergenic 35.0%
TGAAAATCCTTCAATGGAAG+AGG - chr4.1:46908502-46908521 MS.gene61654:intron 35.0%
TGGATATTGGTCGATAAAAG+AGG - chr4.1:46908634-46908653 MS.gene61654:intron 35.0%
TGTTGCATTTACAACGAGAA+AGG - chr4.1:46908340-46908359 MS.gene61654:CDS 35.0%
TTGAAGAAGTTGGAAAGTTC+TGG - chr4.1:46908614-46908633 MS.gene61654:intron 35.0%
TTGTCCTCAAAGACAAAAGA+AGG + chr4.1:46908921-46908940 None:intergenic 35.0%
! ACGGGAAAACCATTTTGTTA+TGG - chr4.1:46908437-46908456 MS.gene61654:intron 35.0%
! GATGGGTTTCCATAACAAAA+TGG + chr4.1:46908449-46908468 None:intergenic 35.0%
! TAACAAAATGGTTTTCCCGT+TGG + chr4.1:46908437-46908456 None:intergenic 35.0%
!! AGTTGGAAAGTTCTGGATAT+TGG - chr4.1:46908621-46908640 MS.gene61654:intron 35.0%
!! TTTCCTTTTGATGACCTCTT+CGG + chr4.1:46908275-46908294 None:intergenic 35.0%
ACACAAGGAGTCAACCTTAT+TGG - chr4.1:46909326-46909345 MS.gene61654:CDS 40.0%
ACCAATGTCTTGAAAACTGG+AGG - chr4.1:46908719-46908738 MS.gene61654:CDS 40.0%
AGTGGAATAGGATATGGAAG+TGG - chr4.1:46909353-46909372 MS.gene61654:CDS 40.0%
ATTCTCATGCTTTCTCCAAC+GGG - chr4.1:46908419-46908438 MS.gene61654:intron 40.0%
CAAGATGTACACATACCATG+AGG + chr4.1:46908755-46908774 None:intergenic 40.0%
CCGAGTTAACAACACTTTCA+GGG - chr4.1:46908384-46908403 MS.gene61654:intron 40.0%
CGAGTTAACAACACTTTCAG+GGG - chr4.1:46908385-46908404 MS.gene61654:intron 40.0%
CGTAGCAATTTCTACCATTG+AGG - chr4.1:46908872-46908891 MS.gene61654:intron 40.0%
GGAAGAGGAAATGATGAAAC+CGG - chr4.1:46908517-46908536 MS.gene61654:intron 40.0%
GGTGTAAGTGGAATAGGATA+TGG - chr4.1:46909347-46909366 MS.gene61654:CDS 40.0%
GTCAACCTTATTGGTGTAAG+TGG - chr4.1:46909335-46909354 MS.gene61654:CDS 40.0%
TCCGAGTTAACAACACTTTC+AGG - chr4.1:46908383-46908402 MS.gene61654:intron 40.0%
TCTACCATTGAGGAACTGAA+TGG - chr4.1:46908882-46908901 MS.gene61654:intron 40.0%
TGCACCAACTTCATGATCTT+TGG + chr4.1:46908946-46908965 None:intergenic 40.0%
! GCAACCATACCTTTACCTTT+TGG + chr4.1:46909275-46909294 None:intergenic 40.0%
!! CCCTGAAAGTGTTGTTAACT+CGG + chr4.1:46908387-46908406 None:intergenic 40.0%
!! TCCTCCAGTTTTCAAGACAT+TGG + chr4.1:46908723-46908742 None:intergenic 40.0%
!! TGAAAGTGTTGTTAACTCGG+AGG + chr4.1:46908384-46908403 None:intergenic 40.0%
GACTCCATTCAGTTCCTCAA+TGG + chr4.1:46908889-46908908 None:intergenic 45.0%
GCATTTACAACGAGAAAGGC+TGG - chr4.1:46908344-46908363 MS.gene61654:CDS 45.0%
! AATGTGTGAGTGTTGCCGCT+AGG + chr4.1:46909023-46909042 None:intergenic 50.0%
GCGCCGAAGAGGTCATCAAA+AGG - chr4.1:46908269-46908288 MS.gene61654:CDS 55.0%
GGAGGAGAGATTGACCCTCA+TGG - chr4.1:46908737-46908756 MS.gene61654:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 46908266 46909403 46908266 ID=MS.gene61654
chr4.1 mRNA 46908266 46909403 46908266 ID=MS.gene61654.t1;Parent=MS.gene61654
chr4.1 exon 46908911 46909403 46908911 ID=MS.gene61654.t1.exon1;Parent=MS.gene61654.t1
chr4.1 CDS 46908911 46909403 46908911 ID=cds.MS.gene61654.t1;Parent=MS.gene61654.t1
chr4.1 exon 46908703 46908792 46908703 ID=MS.gene61654.t1.exon2;Parent=MS.gene61654.t1
chr4.1 CDS 46908703 46908792 46908703 ID=cds.MS.gene61654.t1;Parent=MS.gene61654.t1
chr4.1 exon 46908266 46908390 46908266 ID=MS.gene61654.t1.exon3;Parent=MS.gene61654.t1
chr4.1 CDS 46908266 46908390 46908266 ID=cds.MS.gene61654.t1;Parent=MS.gene61654.t1
Gene Sequence

>MS.gene61654

ATGGCGCCGAAGAGGTCATCAAAAGGAAAACAAAAAATTGAGTTGAAAAAAATTGAGAAAAAGGATGATCTCTTTGTTGCATTTACAACGAGAAAGGCTGGAATTTACAACAAGGCCTCCGAGTTAACAACACTTTCAGGGGCCTCAGTTGATATTCTCATGCTTTCTCCAACGGGAAAACCATTTTGTTATGGAAACCCATCTTCTGAATCTATAACAAAAACTAGTTTGAAGGATGAAAATCCTTCAATGGAAGAGGAAATGATGAAACCGGAGATTATAGAGCAAAATGAAAAAAATGATGATCTTTTGGATAAAAAGCATGTTGCGGAAGCACAACGTAAAAATTTGAAGAAGTTGGAAAGTTCTGGATATTGGTCGATAAAAGAGGACCAATATGATCAAGTGAAAGAAATTGACAAATCCTTAGTTGCTGTCGTAAATAAATTGATAACCAATGTCTTGAAAACTGGAGGAGAGATTGACCCTCATGGTATGTGTACATCTTGTAACTATCTGTGTTATATTTGATCTATTAATGACGTCAAATGATTTCATGGCATCCTTTTTACCTAATTATTTTAAGTGAATCTGGTTATAATTTTTCGTAGCAATTTCTACCATTGAGGAACTGAATGGAGTCAAAACAAATCCTTCTTTTGTCTTTGAGGACAACACCAAAGATCATGAAGTTGGTGCAAGTAAGAATTTTCATTCATGTTTTTAATTTTTTTTATTTTTTTTCATGAAATATCCTAGCGGCAACACTCACACATTAAACTTTTAGACAAGATCAGATGTTGTTGGATAATCATGTCCCAACACATCAAATATCTCTAGATAAAACTTTTACCATTTAAGTTGCACTTATGGGAAAAATCTCAATTCTTTTTCTTCTTAATTTCCATTACTAGGCATGCAAACATTTACTTTTAATATTTCATGCGACAAACCTAACATATATATTATGTATTGTCTTTCCTTTCATTGATTCAATTTTTTTTATCCAAAAGGTAAAGGTATGGTTGCTAAAGCCTCTCAAGATATAGCTACAAAAAAAACACAAGGAGTCAACCTTATTGGTGTAAGTGGAATAGGATATGGAAGTGGTGTTGATCATGCTTCTCCAGATATATGA

Protein sequence

>MS.gene61654.t1

MAPKRSSKGKQKIELKKIEKKDDLFVAFTTRKAGIYNKASELTTLSGASVDILMLSPTGKPFCYGNPSSESITKTSLKDENPSMEEEMMKPEIIEQNEKNDDLLDKKHVAEAQRKNLKKLESSGYWSIKEDQYDQVKEIDKSLVAVVNKLITNVLKTGGEIDPHAISTIEELNGVKTNPSFVFEDNTKDHEVGASKGMVAKASQDIATKKTQGVNLIGVSGIGYGSGVDHASPDI