AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene61733

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61733.t1 MTR_4g128340 97.656 128 3 0 1 128 42 169 1.26e-90 259
MS.gene61733.t1 MTR_2g104900 35.632 174 62 6 1 128 78 247 1.46e-18 78.6
MS.gene61733.t1 MTR_1g033340 35.443 158 66 6 4 128 97 251 2.70e-18 77.8
MS.gene61733.t1 MTR_5g077370 67.273 55 15 1 4 55 87 141 7.45e-18 76.6
MS.gene61733.t1 MTR_3g065020 64.286 56 19 1 3 58 85 139 4.77e-17 74.3
MS.gene61733.t1 MTR_4g006575 67.347 49 14 1 1 47 64 112 1.33e-16 73.2
MS.gene61733.t1 MTR_4g006600 67.347 49 14 1 1 47 64 112 1.33e-16 73.2
MS.gene61733.t1 MTR_1g013130 64.583 48 17 0 1 48 91 138 2.30e-16 73.2
MS.gene61733.t1 MTR_3g106230 73.684 38 10 0 2 39 70 107 8.16e-15 68.2
MS.gene61733.t1 MTR_7g086860 74.286 35 9 0 4 38 30 64 4.09e-13 62.4
MS.gene61733.t1 MTR_1g107055 70.588 34 10 0 4 37 64 97 4.49e-12 60.5
MS.gene61733.t1 MTR_7g083210 75.758 33 8 0 4 36 39 71 5.93e-12 60.1
MS.gene61733.t1 MTR_7g022250 29.936 157 78 5 3 128 84 239 1.84e-11 59.3
MS.gene61733.t1 MTR_8g466760 61.538 39 15 0 2 40 96 134 3.43e-11 59.3
MS.gene61733.t1 MTR_7g028915 31.159 138 78 3 3 128 80 212 4.57e-11 58.2
MS.gene61733.t1 MTR_5g008390 57.500 40 17 0 1 40 11 50 8.42e-11 55.1
MS.gene61733.t1 MTR_3g009410 61.538 39 15 0 2 40 96 134 9.02e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61733.t1 AT5G57520 47.287 129 43 6 1 128 46 150 1.89e-22 86.7
MS.gene61733.t1 AT5G10970 45.714 105 46 4 2 106 100 193 2.14e-19 81.3
MS.gene61733.t1 AT5G25160 54.545 88 25 5 2 85 57 133 9.75e-19 79.0
MS.gene61733.t1 AT1G66140 75.000 44 11 0 1 44 80 123 2.20e-17 75.9
MS.gene61733.t1 AT1G80730 54.054 74 30 1 2 71 64 137 4.82e-17 74.3
MS.gene61733.t1 AT1G24625 78.947 38 8 0 4 41 57 94 3.54e-16 71.6
MS.gene61733.t1 AT5G14010 31.783 129 81 2 4 128 36 161 5.43e-13 62.4
MS.gene61733.t1 AT5G01860 53.061 49 19 1 4 52 65 109 2.92e-12 61.2

Find 32 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAGGAAGGTGTGGCTTCT+TGG 0.263748 8.1:-4621251 MS.gene61733:CDS
TTTCAAGCTTGTGAGCATTT+TGG 0.263848 8.1:+4621469 None:intergenic
GAGAAGTTTGAAGGGTGTTC+TGG 0.318303 8.1:+4621384 None:intergenic
GTTTAGTTATGGAAATTCTA+AGG 0.345981 8.1:-4621268 MS.gene61733:CDS
TTCTCTACTAATTACCATCT+TGG 0.347245 8.1:-4621366 MS.gene61733:CDS
AGAAAAGATCAGTTTAGTTA+TGG 0.369153 8.1:-4621279 MS.gene61733:CDS
TCATCACATGACCTTGTCTA+TGG 0.389808 8.1:+4621313 None:intergenic
GCCAACTTGATTTGTCCTTA+AGG 0.395324 8.1:-4621182 MS.gene61733:CDS
CATTTATAATCAGAGCCTTA+AGG 0.403759 8.1:+4621167 None:intergenic
TAGTACTTCTGAAAATGTTC+AGG 0.411786 8.1:-4621214 MS.gene61733:CDS
AGGTGTGGCTTCTTGGTCAA+GGG 0.430940 8.1:-4621244 MS.gene61733:CDS
TAATTAGTAGAGAAGTTTGA+AGG 0.473837 8.1:+4621375 None:intergenic
AAGGTGTGGCTTCTTGGTCA+AGG 0.476344 8.1:-4621245 MS.gene61733:CDS
AGTTATGGAAATTCTAAGGA+AGG 0.495820 8.1:-4621264 MS.gene61733:CDS
GGACACTTGCAAAGAAGAGT+AGG 0.507806 8.1:-4621446 MS.gene61733:CDS
AAAATGCTCACAAGCTTGAA+AGG 0.509103 8.1:-4621467 MS.gene61733:CDS
AATTACCATCTTGGAAGTCA+TGG 0.516260 8.1:-4621357 MS.gene61733:CDS
CATTTCCATGACTTCCAAGA+TGG 0.523980 8.1:+4621352 None:intergenic
AATTAGTAGAGAAGTTTGAA+GGG 0.551396 8.1:+4621376 None:intergenic
TGGAAATTCTAAGGAAGGTG+TGG 0.568793 8.1:-4621259 MS.gene61733:CDS
GCCTTAAGGACAAATCAAGT+TGG 0.573380 8.1:+4621181 None:intergenic
GACACTTGCAAAGAAGAGTA+GGG 0.576451 8.1:-4621445 MS.gene61733:CDS
CATCTTCATCTATGGAGCAA+AGG 0.583885 8.1:-4621551 None:intergenic
GGTGTGGCTTCTTGGTCAAG+GGG 0.586234 8.1:-4621243 MS.gene61733:CDS
TATAGCTCTCAAGCTTTGGG+AGG 0.587863 8.1:-4621495 MS.gene61733:CDS
TACTTCTGAAAATGTTCAGG+AGG 0.608835 8.1:-4621211 MS.gene61733:CDS
TTCAGCATAAGAAGATTGCA+TGG 0.617834 8.1:+4621410 None:intergenic
ATAGACAAGGTCATGTGATG+AGG 0.622400 8.1:-4621311 MS.gene61733:CDS
CTTGACAACAACCATAGACA+AGG 0.646735 8.1:-4621324 MS.gene61733:CDS
CAAGGTCATGTGATGAGGCA+TGG 0.652675 8.1:-4621306 MS.gene61733:CDS
GGTCATGTGATGAGGCATGG+TGG 0.708373 8.1:-4621303 MS.gene61733:CDS
AGCATAAGAAGATTGCATGG+CGG 0.769594 8.1:+4621413 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATTAGTAGAGAAGTTTGAA+GGG + chr8.1:4621343-4621362 None:intergenic 25.0%
! AGAAAAGATCAGTTTAGTTA+TGG - chr8.1:4621437-4621456 MS.gene61733:CDS 25.0%
! GTTTAGTTATGGAAATTCTA+AGG - chr8.1:4621448-4621467 MS.gene61733:CDS 25.0%
! TAATTAGTAGAGAAGTTTGA+AGG + chr8.1:4621344-4621363 None:intergenic 25.0%
TTCTCTACTAATTACCATCT+TGG - chr8.1:4621350-4621369 MS.gene61733:CDS 30.0%
! AGTTATGGAAATTCTAAGGA+AGG - chr8.1:4621452-4621471 MS.gene61733:CDS 30.0%
! TAGTACTTCTGAAAATGTTC+AGG - chr8.1:4621502-4621521 MS.gene61733:CDS 30.0%
! TTTTATAGCTCTCAAGCTTT+GGG - chr8.1:4621218-4621237 MS.gene61733:CDS 30.0%
AAAATGCTCACAAGCTTGAA+AGG - chr8.1:4621249-4621268 MS.gene61733:CDS 35.0%
AATTACCATCTTGGAAGTCA+TGG - chr8.1:4621359-4621378 MS.gene61733:CDS 35.0%
TTCAGCATAAGAAGATTGCA+TGG + chr8.1:4621309-4621328 None:intergenic 35.0%
! GTTTTATAGCTCTCAAGCTT+TGG - chr8.1:4621217-4621236 MS.gene61733:CDS 35.0%
! TACTTCTGAAAATGTTCAGG+AGG - chr8.1:4621505-4621524 MS.gene61733:CDS 35.0%
! TTTCAAGCTTGTGAGCATTT+TGG + chr8.1:4621250-4621269 None:intergenic 35.0%
AGCATAAGAAGATTGCATGG+CGG + chr8.1:4621306-4621325 None:intergenic 40.0%
ATAGACAAGGTCATGTGATG+AGG - chr8.1:4621405-4621424 MS.gene61733:CDS 40.0%
CATTTCCATGACTTCCAAGA+TGG + chr8.1:4621367-4621386 None:intergenic 40.0%
CTTGACAACAACCATAGACA+AGG - chr8.1:4621392-4621411 MS.gene61733:CDS 40.0%
GACACTTGCAAAGAAGAGTA+GGG - chr8.1:4621271-4621290 MS.gene61733:CDS 40.0%
GCCTTAAGGACAAATCAAGT+TGG + chr8.1:4621538-4621557 None:intergenic 40.0%
TCATCACATGACCTTGTCTA+TGG + chr8.1:4621406-4621425 None:intergenic 40.0%
! TGGAAATTCTAAGGAAGGTG+TGG - chr8.1:4621457-4621476 MS.gene61733:CDS 40.0%
!! GCCAACTTGATTTGTCCTTA+AGG - chr8.1:4621534-4621553 MS.gene61733:CDS 40.0%
GGACACTTGCAAAGAAGAGT+AGG - chr8.1:4621270-4621289 MS.gene61733:CDS 45.0%
!! GAGAAGTTTGAAGGGTGTTC+TGG + chr8.1:4621335-4621354 None:intergenic 45.0%
!! TATAGCTCTCAAGCTTTGGG+AGG - chr8.1:4621221-4621240 MS.gene61733:CDS 45.0%
AAGGTGTGGCTTCTTGGTCA+AGG - chr8.1:4621471-4621490 MS.gene61733:CDS 50.0%
AGGTGTGGCTTCTTGGTCAA+GGG - chr8.1:4621472-4621491 MS.gene61733:CDS 50.0%
CAAGGTCATGTGATGAGGCA+TGG - chr8.1:4621410-4621429 MS.gene61733:CDS 50.0%
! CTAAGGAAGGTGTGGCTTCT+TGG - chr8.1:4621465-4621484 MS.gene61733:CDS 50.0%
GGTCATGTGATGAGGCATGG+TGG - chr8.1:4621413-4621432 MS.gene61733:CDS 55.0%
GGTGTGGCTTCTTGGTCAAG+GGG - chr8.1:4621473-4621492 MS.gene61733:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 4621176 4621562 4621176 ID=MS.gene61733
chr8.1 mRNA 4621176 4621562 4621176 ID=MS.gene61733.t1;Parent=MS.gene61733
chr8.1 exon 4621176 4621562 4621176 ID=MS.gene61733.t1.exon1;Parent=MS.gene61733.t1
chr8.1 CDS 4621176 4621562 4621176 ID=cds.MS.gene61733.t1;Parent=MS.gene61733.t1
Gene Sequence

>MS.gene61733

ATGGAGCAAAGGATATTCTCATGCAATTATTGTCAGAGAAAGTTTTATAGCTCTCAAGCTTTGGGAGGACACCAAAATGCTCACAAGCTTGAAAGGACACTTGCAAAGAAGAGTAGGGAGATGAGTTCCGCCATGCAATCTTCTTATGCTGAATTACCAGAACACCCTTCAAACTTCTCTACTAATTACCATCTTGGAAGTCATGGAAATGCTCATCTTGACAACAACCATAGACAAGGTCATGTGATGAGGCATGGTGGAAGAAAAGATCAGTTTAGTTATGGAAATTCTAAGGAAGGTGTGGCTTCTTGGTCAAGGGGTTATAATAGTACTTCTGAAAATGTTCAGGAGGATATTAGCCAACTTGATTTGTCCTTAAGGCTCTGA
Protein sequence

>MS.gene61733.t1

MEQRIFSCNYCQRKFYSSQALGGHQNAHKLERTLAKKSREMSSAMQSSYAELPEHPSNFSTNYHLGSHGNAHLDNNHRQGHVMRHGGRKDQFSYGNSKEGVASWSRGYNSTSENVQEDISQLDLSLRL