Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61747.t1 | PNX93400.1 | 75.9 | 158 | 24 | 1 | 24 | 167 | 85 | 242 | 2.10E-63 | 251.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61747.t1 | Q84MZ4 | 39.7 | 194 | 66 | 1 | 25 | 167 | 128 | 321 | 5.4e-36 | 152.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61747.t1 | A0A2K3NGZ1 | 75.9 | 158 | 24 | 1 | 24 | 167 | 24 | 181 | 1.5e-63 | 251.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene61747.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61747.t1 | AT1G72050 | 42.268 | 194 | 61 | 2 | 25 | 167 | 40 | 233 | 4.71e-44 | 148 |
| MS.gene61747.t1 | AT1G72050 | 42.268 | 194 | 61 | 2 | 25 | 167 | 40 | 233 | 5.42e-44 | 148 |
| MS.gene61747.t1 | AT1G72050 | 42.268 | 194 | 61 | 2 | 25 | 167 | 128 | 321 | 1.88e-43 | 149 |
| MS.gene61747.t1 | AT1G72050 | 42.268 | 194 | 61 | 2 | 25 | 167 | 143 | 336 | 2.27e-43 | 149 |
Find 35 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTTCCCACAGCCAATTTC+CGG | 0.209602 | 8.4:-58667253 | None:intergenic |
| AACTACCAGTTTCATGTTTA+CGG | 0.285852 | 8.4:-58667613 | None:intergenic |
| CCGCACGTGTGCCCGGAAAT+TGG | 0.292837 | 8.4:+58667241 | MS.gene61747:CDS |
| GGTGTTTCATTGTGATTATA+AGG | 0.329936 | 8.4:+58667643 | MS.gene61747:CDS |
| CGTGACTGAGGCACCCTTGA+TGG | 0.357763 | 8.4:-58666881 | None:intergenic |
| AGGATTTAGTGTGTGCTCTA+AGG | 0.367395 | 8.4:-58667523 | None:intergenic |
| CCTTCCCACAGCCAATTTCC+GGG | 0.385128 | 8.4:-58667252 | None:intergenic |
| CTCACTTTCTGCAGTTAAGC+TGG | 0.403175 | 8.4:+58667439 | MS.gene61747:intron |
| CACCTCCGTAAACATGAAAC+TGG | 0.428491 | 8.4:+58667608 | MS.gene61747:CDS |
| GACTCCAAAATGCGTGAGGC+AGG | 0.464338 | 8.4:+58666841 | None:intergenic |
| AAGTCTCACAGTTCACATAC+TGG | 0.472897 | 8.4:-58667550 | None:intergenic |
| TCAGAAGCCGCACGTGTGCC+CGG | 0.477093 | 8.4:+58667234 | MS.gene61747:CDS |
| ATGTGAACTGTGAGACTTGT+GGG | 0.494220 | 8.4:+58667555 | MS.gene61747:CDS |
| GTAAAACTCTTCATACAGCC+AGG | 0.508670 | 8.4:-58667488 | None:intergenic |
| GAGGTAGTGTGCTTATACCC+TGG | 0.518727 | 8.4:+58667470 | MS.gene61747:CDS |
| AGGATTTCTTACACTTGAGA+AGG | 0.521556 | 8.4:-58667678 | None:intergenic |
| CCTCTTGAGAAGCTCCATCA+AGG | 0.528923 | 8.4:+58666867 | MS.gene61747:CDS |
| GTGTGCCCGGAAATTGGCTG+TGG | 0.529847 | 8.4:+58667247 | MS.gene61747:CDS |
| AGTTAAGCTGGAATCAATAG+AGG | 0.553788 | 8.4:+58667451 | MS.gene61747:intron |
| CCCGGAAATTGGCTGTGGGA+AGG | 0.577142 | 8.4:+58667252 | MS.gene61747:CDS |
| ACAGTTCACATACTGGTGGC+AGG | 0.578620 | 8.4:-58667543 | None:intergenic |
| GTCACGCTCCGTTCAAAACG+AGG | 0.580544 | 8.4:+58666898 | MS.gene61747:CDS |
| GAGAAGCACAAACATTCTCA+TGG | 0.599567 | 8.4:+58667298 | MS.gene61747:CDS |
| CTCTTGAGAAGCTCCATCAA+GGG | 0.600460 | 8.4:+58666868 | MS.gene61747:CDS |
| CCAATTTCCGGGCACACGTG+CGG | 0.601234 | 8.4:-58667241 | None:intergenic |
| AAACATGACAAGACATGTCA+AGG | 0.604864 | 8.4:+58667168 | MS.gene61747:CDS |
| CCTTGATGGAGCTTCTCAAG+AGG | 0.605639 | 8.4:-58666867 | None:intergenic |
| TGTCAAGGAGTTTCATAACA+AGG | 0.621209 | 8.4:+58667183 | MS.gene61747:CDS |
| TAAAACTCTTCATACAGCCA+GGG | 0.647065 | 8.4:-58667487 | None:intergenic |
| TGAAACTGGTAGTTCATCAG+AGG | 0.651126 | 8.4:+58667622 | MS.gene61747:CDS |
| CTCAGACTCCAAAATGCGTG+AGG | 0.655396 | 8.4:+58666837 | None:intergenic |
| TACCAGTTTCATGTTTACGG+AGG | 0.669845 | 8.4:-58667610 | None:intergenic |
| TGTGCCCGGAAATTGGCTGT+GGG | 0.690309 | 8.4:+58667248 | MS.gene61747:CDS |
| TCTCACAGTTCACATACTGG+TGG | 0.715768 | 8.4:-58667547 | None:intergenic |
| TATGTGAACTGTGAGACTTG+TGG | 0.750818 | 8.4:+58667554 | MS.gene61747:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTACATGTTTTATATTTCT+GGG | + | chr8.4:58667329-58667348 | MS.gene61747:intron | 15.0% |
| !!! | CTTATTAAGTTATTAGTTTA+TGG | + | chr8.4:58666970-58666989 | MS.gene61747:intron | 15.0% |
| !!! | CATTACATGTTTTATATTTC+TGG | + | chr8.4:58667328-58667347 | MS.gene61747:intron | 20.0% |
| ! | TCATCAATAGTGCAAATATA+CGG | - | chr8.4:58667034-58667053 | None:intergenic | 25.0% |
| !! | GTTTTCAGAACTCTAAATAA+AGG | - | chr8.4:58667386-58667405 | None:intergenic | 25.0% |
| AACTACCAGTTTCATGTTTA+CGG | - | chr8.4:58667616-58667635 | None:intergenic | 30.0% | |
| GGTGTTTCATTGTGATTATA+AGG | + | chr8.4:58667643-58667662 | MS.gene61747:CDS | 30.0% | |
| ! | AATCGCGATTTTTCTTTGAA+GGG | + | chr8.4:58667145-58667164 | MS.gene61747:CDS | 30.0% |
| AAACATGACAAGACATGTCA+AGG | + | chr8.4:58667168-58667187 | MS.gene61747:CDS | 35.0% | |
| ACTCATGGCTTATTCTTGTT+GGG | + | chr8.4:58666936-58666955 | MS.gene61747:intron | 35.0% | |
| AGGATTTCTTACACTTGAGA+AGG | - | chr8.4:58667681-58667700 | None:intergenic | 35.0% | |
| AGTTAAGCTGGAATCAATAG+AGG | + | chr8.4:58667451-58667470 | MS.gene61747:intron | 35.0% | |
| GTAGAAGATGACGATTCAAA+TGG | - | chr8.4:58667084-58667103 | None:intergenic | 35.0% | |
| GTCATCTTCTACAACATGAA+GGG | + | chr8.4:58667092-58667111 | MS.gene61747:intron | 35.0% | |
| TAAAACTCTTCATACAGCCA+GGG | - | chr8.4:58667490-58667509 | None:intergenic | 35.0% | |
| TCATCTTCTACAACATGAAG+GGG | + | chr8.4:58667093-58667112 | MS.gene61747:intron | 35.0% | |
| TGTCAAGGAGTTTCATAACA+AGG | + | chr8.4:58667183-58667202 | MS.gene61747:CDS | 35.0% | |
| ! | CAATCGCGATTTTTCTTTGA+AGG | + | chr8.4:58667144-58667163 | MS.gene61747:CDS | 35.0% |
| ! | TTGCACTATTGATGATTGTC+AGG | + | chr8.4:58667039-58667058 | MS.gene61747:intron | 35.0% |
| !! | TCTGAATTTTGACATCAGCA+GGG | - | chr8.4:58667219-58667238 | None:intergenic | 35.0% |
| !! | TTCTGAATTTTGACATCAGC+AGG | - | chr8.4:58667220-58667239 | None:intergenic | 35.0% |
| !!! | CATTTTTTTTCGTCTTCCAG+AGG | + | chr8.4:58667008-58667027 | MS.gene61747:intron | 35.0% |
| !!! | TTGGTAGTTTTGTTCACTCA+TGG | + | chr8.4:58666921-58666940 | MS.gene61747:intron | 35.0% |
| AAGTCTCACAGTTCACATAC+TGG | - | chr8.4:58667553-58667572 | None:intergenic | 40.0% | |
| AGGATTTAGTGTGTGCTCTA+AGG | - | chr8.4:58667526-58667545 | None:intergenic | 40.0% | |
| ATGTGAACTGTGAGACTTGT+GGG | + | chr8.4:58667555-58667574 | MS.gene61747:CDS | 40.0% | |
| CACTCATGGCTTATTCTTGT+TGG | + | chr8.4:58666935-58666954 | MS.gene61747:intron | 40.0% | |
| CGTCATCTTCTACAACATGA+AGG | + | chr8.4:58667091-58667110 | MS.gene61747:intron | 40.0% | |
| CTCATGGCTTATTCTTGTTG+GGG | + | chr8.4:58666937-58666956 | MS.gene61747:intron | 40.0% | |
| GTAAAACTCTTCATACAGCC+AGG | - | chr8.4:58667491-58667510 | None:intergenic | 40.0% | |
| TACCAGTTTCATGTTTACGG+AGG | - | chr8.4:58667613-58667632 | None:intergenic | 40.0% | |
| TATGTGAACTGTGAGACTTG+TGG | + | chr8.4:58667554-58667573 | MS.gene61747:CDS | 40.0% | |
| TCAGGCAAGTTACAGAAGAA+AGG | + | chr8.4:58667057-58667076 | MS.gene61747:intron | 40.0% | |
| TGAAACTGGTAGTTCATCAG+AGG | + | chr8.4:58667622-58667641 | MS.gene61747:CDS | 40.0% | |
| ! | GAGAAGCACAAACATTCTCA+TGG | + | chr8.4:58667298-58667317 | MS.gene61747:CDS | 40.0% |
| !! | ACTACCAACCTCGTTTTGAA+CGG | - | chr8.4:58666909-58666928 | None:intergenic | 40.0% |
| !! | CTGAATTTTGACATCAGCAG+GGG | - | chr8.4:58667218-58667237 | None:intergenic | 40.0% |
| CACCTCCGTAAACATGAAAC+TGG | + | chr8.4:58667608-58667627 | MS.gene61747:CDS | 45.0% | |
| CTCACTTTCTGCAGTTAAGC+TGG | + | chr8.4:58667439-58667458 | MS.gene61747:intron | 45.0% | |
| CTCTTGAGAAGCTCCATCAA+GGG | + | chr8.4:58666868-58666887 | MS.gene61747:CDS | 45.0% | |
| TAGTGCAAATATACGGCCTC+TGG | - | chr8.4:58667027-58667046 | None:intergenic | 45.0% | |
| TCGCGATTGCAATTCTCAAC+TGG | - | chr8.4:58667133-58667152 | None:intergenic | 45.0% | |
| TCTCACAGTTCACATACTGG+TGG | - | chr8.4:58667550-58667569 | None:intergenic | 45.0% | |
| ACAGTTCACATACTGGTGGC+AGG | - | chr8.4:58667546-58667565 | None:intergenic | 50.0% | |
| CCTCTTGAGAAGCTCCATCA+AGG | + | chr8.4:58666867-58666886 | MS.gene61747:CDS | 50.0% | |
| CCTTGATGGAGCTTCTCAAG+AGG | - | chr8.4:58666870-58666889 | None:intergenic | 50.0% | |
| GAGGTAGTGTGCTTATACCC+TGG | + | chr8.4:58667470-58667489 | MS.gene61747:CDS | 50.0% | |
| ! | TTTTGAACGGAGCGTGACTG+AGG | - | chr8.4:58666896-58666915 | None:intergenic | 50.0% |
| CCTTCCCACAGCCAATTTCC+GGG | - | chr8.4:58667255-58667274 | None:intergenic | 55.0% | |
| CGCTCCGTTCAAAACGAGGT+TGG | + | chr8.4:58666902-58666921 | MS.gene61747:intron | 55.0% | |
| GCCTTCCCACAGCCAATTTC+CGG | - | chr8.4:58667256-58667275 | None:intergenic | 55.0% | |
| GTCACGCTCCGTTCAAAACG+AGG | + | chr8.4:58666898-58666917 | MS.gene61747:CDS | 55.0% | |
| TGTGCCCGGAAATTGGCTGT+GGG | + | chr8.4:58667248-58667267 | MS.gene61747:CDS | 55.0% | |
| CCAATTTCCGGGCACACGTG+CGG | - | chr8.4:58667244-58667263 | None:intergenic | 60.0% | |
| CCCGGAAATTGGCTGTGGGA+AGG | + | chr8.4:58667252-58667271 | MS.gene61747:CDS | 60.0% | |
| GTGTGCCCGGAAATTGGCTG+TGG | + | chr8.4:58667247-58667266 | MS.gene61747:CDS | 60.0% | |
| ! | CGTGACTGAGGCACCCTTGA+TGG | - | chr8.4:58666884-58666903 | None:intergenic | 60.0% |
| CCGCACGTGTGCCCGGAAAT+TGG | + | chr8.4:58667241-58667260 | MS.gene61747:CDS | 65.0% | |
| TCAGAAGCCGCACGTGTGCC+CGG | + | chr8.4:58667234-58667253 | MS.gene61747:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 58666850 | 58667703 | 58666850 | ID=MS.gene61747 |
| chr8.4 | mRNA | 58666850 | 58667703 | 58666850 | ID=MS.gene61747.t1;Parent=MS.gene61747 |
| chr8.4 | exon | 58666850 | 58666919 | 58666850 | ID=MS.gene61747.t1.exon1;Parent=MS.gene61747.t1 |
| chr8.4 | CDS | 58666850 | 58666919 | 58666850 | ID=cds.MS.gene61747.t1;Parent=MS.gene61747.t1 |
| chr8.4 | exon | 58667134 | 58667319 | 58667134 | ID=MS.gene61747.t1.exon2;Parent=MS.gene61747.t1 |
| chr8.4 | CDS | 58667134 | 58667319 | 58667134 | ID=cds.MS.gene61747.t1;Parent=MS.gene61747.t1 |
| chr8.4 | exon | 58667453 | 58667703 | 58667453 | ID=MS.gene61747.t1.exon3;Parent=MS.gene61747.t1 |
| chr8.4 | CDS | 58667453 | 58667703 | 58667453 | ID=cds.MS.gene61747.t1;Parent=MS.gene61747.t1 |
| Gene Sequence |
| Protein sequence |
