Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62310.t1 | XP_024636802.1 | 83.8 | 105 | 17 | 0 | 1 | 105 | 64 | 168 | 5.90E-19 | 104 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62310.t1 | A0A396IJH9 | 83.8 | 105 | 17 | 0 | 1 | 105 | 64 | 168 | 4.2e-19 | 104.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene62310.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62310.t1 | MTR_3g084720 | 57.143 | 63 | 26 | 1 | 44 | 105 | 106 | 168 | 8.96e-18 | 76.6 |
| MS.gene62310.t1 | MTR_6g465230 | 67.857 | 56 | 18 | 0 | 1 | 56 | 163 | 218 | 5.16e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62310.t1 | AT2G18490 | 67.391 | 46 | 15 | 0 | 58 | 103 | 155 | 200 | 3.94e-17 | 75.5 |
| MS.gene62310.t1 | AT2G18490 | 60.377 | 53 | 21 | 0 | 51 | 103 | 198 | 250 | 5.44e-17 | 75.9 |
| MS.gene62310.t1 | AT2G15740 | 63.265 | 49 | 18 | 0 | 57 | 105 | 229 | 277 | 1.48e-16 | 74.7 |
| MS.gene62310.t1 | AT5G22990 | 61.538 | 52 | 19 | 1 | 53 | 103 | 227 | 278 | 1.96e-14 | 68.9 |
| MS.gene62310.t1 | AT5G42640 | 50.877 | 57 | 24 | 1 | 51 | 103 | 218 | 274 | 2.49e-14 | 68.6 |
Find 20 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTAGCAACATCACAAAATT+TGG | 0.259907 | 8.2:+13736385 | MS.gene62310:CDS |
| AATGATGGAACAACTACTTT+TGG | 0.332371 | 8.2:+13736511 | MS.gene62310:CDS |
| AACAATACTGTTGCTACTAA+TGG | 0.363944 | 8.2:+13736233 | MS.gene62310:CDS |
| CTACTAATGGTGATGAAATC+CGG | 0.385370 | 8.2:+13736246 | MS.gene62310:CDS |
| TTACACTTTGGACACGTATA+CGG | 0.389805 | 8.2:-13736356 | None:intergenic |
| CGATTTCAAAAGCTCAATGA+TGG | 0.441823 | 8.2:+13736496 | MS.gene62310:CDS |
| GACGAGCCTCCAAGGAGATC+AGG | 0.460583 | 8.2:-13736179 | None:intergenic |
| GCTAGTACTTTGTTACACTT+TGG | 0.466837 | 8.2:-13736368 | None:intergenic |
| GCTCATCCTGATCTCCTTGG+AGG | 0.493768 | 8.2:+13736173 | MS.gene62310:CDS |
| TATGCTCATCCTGATCTCCT+TGG | 0.493878 | 8.2:+13736170 | MS.gene62310:CDS |
| AAAGAAGAAATTGTTGATGA+TGG | 0.494936 | 8.2:+13736299 | MS.gene62310:CDS |
| ACAGTATTGTTTACCTCAGA+TGG | 0.506108 | 8.2:-13736221 | None:intergenic |
| TGTTGGTATTATTAGAAGCC+CGG | 0.523272 | 8.2:-13736265 | None:intergenic |
| GACTGATCAACAGAAGCAAT+AGG | 0.525212 | 8.2:-13736535 | None:intergenic |
| TACTAATGGTGATGAAATCC+GGG | 0.531216 | 8.2:+13736247 | MS.gene62310:CDS |
| AAAGTGAAGACGAGCCTCCA+AGG | 0.567472 | 8.2:-13736187 | None:intergenic |
| CTAATAATACCAACATGAAG+CGG | 0.574702 | 8.2:+13736273 | MS.gene62310:CDS |
| GAGATCAGGATGAGCATAAG+CGG | 0.608512 | 8.2:-13736165 | None:intergenic |
| TTCACAACAACCGCCATCTG+AGG | 0.636470 | 8.2:+13736208 | MS.gene62310:CDS |
| GTATTGTTTACCTCAGATGG+CGG | 0.675983 | 8.2:-13736218 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAAGAAATTGTTGATGA+TGG | + | chr8.2:13736299-13736318 | MS.gene62310:CDS | 25.0% |
| AACAATACTGTTGCTACTAA+TGG | + | chr8.2:13736233-13736252 | MS.gene62310:CDS | 30.0% | |
| ACTAGCAACATCACAAAATT+TGG | + | chr8.2:13736385-13736404 | MS.gene62310:CDS | 30.0% | |
| AGAAAAAGAGATACAAGTCT+AGG | + | chr8.2:13736452-13736471 | MS.gene62310:CDS | 30.0% | |
| AGTCTACCACACAATAAAAA+TGG | + | chr8.2:13736332-13736351 | MS.gene62310:CDS | 30.0% | |
| CTAATAATACCAACATGAAG+CGG | + | chr8.2:13736273-13736292 | MS.gene62310:CDS | 30.0% | |
| !! | AATGATGGAACAACTACTTT+TGG | + | chr8.2:13736511-13736530 | MS.gene62310:CDS | 30.0% |
| ACAGTATTGTTTACCTCAGA+TGG | - | chr8.2:13736224-13736243 | None:intergenic | 35.0% | |
| CGATTTCAAAAGCTCAATGA+TGG | + | chr8.2:13736496-13736515 | MS.gene62310:CDS | 35.0% | |
| GCTAGTACTTTGTTACACTT+TGG | - | chr8.2:13736371-13736390 | None:intergenic | 35.0% | |
| TGTTGGTATTATTAGAAGCC+CGG | - | chr8.2:13736268-13736287 | None:intergenic | 35.0% | |
| TTACACTTTGGACACGTATA+CGG | - | chr8.2:13736359-13736378 | None:intergenic | 35.0% | |
| ! | CTACTAATGGTGATGAAATC+CGG | + | chr8.2:13736246-13736265 | MS.gene62310:CDS | 35.0% |
| ! | TACTAATGGTGATGAAATCC+GGG | + | chr8.2:13736247-13736266 | MS.gene62310:CDS | 35.0% |
| ! | TTTTATTGTGTGGTAGACTG+TGG | - | chr8.2:13736331-13736350 | None:intergenic | 35.0% |
| !! | TACGGTCCATTTTTATTGTG+TGG | - | chr8.2:13736341-13736360 | None:intergenic | 35.0% |
| !!! | AGCTTTTGAAATCGAAGATC+TGG | - | chr8.2:13736490-13736509 | None:intergenic | 35.0% |
| !!! | TTCTTTTTTCCGCTTCATGT+TGG | - | chr8.2:13736285-13736304 | None:intergenic | 35.0% |
| GACTGATCAACAGAAGCAAT+AGG | - | chr8.2:13736538-13736557 | None:intergenic | 40.0% | |
| GTATTGTTTACCTCAGATGG+CGG | - | chr8.2:13736221-13736240 | None:intergenic | 40.0% | |
| GAGATCAGGATGAGCATAAG+CGG | - | chr8.2:13736168-13736187 | None:intergenic | 45.0% | |
| TATGCTCATCCTGATCTCCT+TGG | + | chr8.2:13736170-13736189 | MS.gene62310:CDS | 45.0% | |
| AAAGTGAAGACGAGCCTCCA+AGG | - | chr8.2:13736190-13736209 | None:intergenic | 50.0% | |
| TTCACAACAACCGCCATCTG+AGG | + | chr8.2:13736208-13736227 | MS.gene62310:CDS | 50.0% | |
| GCTCATCCTGATCTCCTTGG+AGG | + | chr8.2:13736173-13736192 | MS.gene62310:CDS | 55.0% | |
| GACGAGCCTCCAAGGAGATC+AGG | - | chr8.2:13736182-13736201 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 13736146 | 13736570 | 13736146 | ID=MS.gene62310 |
| chr8.2 | mRNA | 13736146 | 13736570 | 13736146 | ID=MS.gene62310.t1;Parent=MS.gene62310 |
| chr8.2 | exon | 13736146 | 13736570 | 13736146 | ID=MS.gene62310.t1.exon1;Parent=MS.gene62310.t1 |
| chr8.2 | CDS | 13736146 | 13736570 | 13736146 | ID=cds.MS.gene62310.t1;Parent=MS.gene62310.t1 |
| Gene Sequence |
| Protein sequence |
