AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62383

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62383.t1 MTR_6g027460 92.167 383 15 3 1 383 1 368 0.0 682
MS.gene62383.t1 MTR_6g027450 73.569 367 59 6 1 367 1 329 0.0 519
MS.gene62383.t1 MTR_7g086780 50.263 380 136 10 15 383 10 347 1.91e-112 335
MS.gene62383.t1 MTR_7g010950 45.876 194 76 6 56 246 94 261 7.57e-43 156
MS.gene62383.t1 MTR_6g012450 32.951 349 165 10 55 383 96 395 5.12e-42 154
MS.gene62383.t1 MTR_4g082060 34.161 322 147 12 9 289 4 301 3.23e-41 151
MS.gene62383.t1 MTR_3g435480 37.736 265 110 10 50 281 98 340 1.58e-40 149
MS.gene62383.t1 MTR_8g044220 33.459 266 114 8 41 289 67 286 8.73e-37 139
MS.gene62383.t1 MTR_5g041420 58.333 96 37 1 55 150 99 191 7.72e-34 128
MS.gene62383.t1 MTR_5g041530 54.286 105 45 1 54 158 100 201 3.15e-30 119
MS.gene62383.t1 MTR_2g016030 77.419 62 14 0 89 150 39 100 3.87e-30 114
MS.gene62383.t1 MTR_5g041380 57.426 101 36 3 65 163 100 195 2.61e-29 117
MS.gene62383.t1 MTR_5g041400 57.778 90 32 2 61 150 89 172 1.04e-28 115
MS.gene62383.t1 MTR_8g068210 47.872 94 36 2 59 152 7 87 4.53e-24 102
MS.gene62383.t1 MTR_8g027295 60.317 63 25 0 92 154 19 81 1.30e-23 99.4
MS.gene62383.t1 MTR_4g088580 44.762 105 48 2 56 152 8 110 2.37e-23 100
MS.gene62383.t1 MTR_4g022370 60.656 61 24 0 90 150 75 135 2.40e-23 100
MS.gene62383.t1 MTR_4g461080 55.844 77 33 1 74 149 5 81 2.66e-23 98.6
MS.gene62383.t1 MTR_2g093220 55.263 76 33 1 76 150 18 93 5.57e-23 98.2
MS.gene62383.t1 MTR_3g091820 54.930 71 32 0 81 151 27 97 5.66e-23 98.2
MS.gene62383.t1 MTR_8g479350 52.703 74 35 0 97 170 68 141 1.35e-22 97.8
MS.gene62383.t1 MTR_2g096740 56.716 67 29 0 84 150 64 130 1.42e-22 97.1
MS.gene62383.t1 MTR_2g096740 56.716 67 29 0 84 150 49 115 1.52e-22 96.7
MS.gene62383.t1 MTR_4g063780 61.667 60 23 0 90 149 68 127 2.14e-22 97.1
MS.gene62383.t1 MTR_8g079060 40.984 122 65 2 97 218 29 143 2.99e-22 94.7
MS.gene62383.t1 MTR_4g089095 48.485 99 35 2 69 152 7 104 8.48e-22 94.7
MS.gene62383.t1 MTR_2g013370 67.241 58 18 1 95 152 44 100 1.44e-21 93.6
MS.gene62383.t1 MTR_2g059540 69.388 49 15 0 97 145 31 79 1.92e-21 91.7
MS.gene62383.t1 MTR_2g014060 54.688 64 29 0 85 148 13 76 2.58e-21 94.0
MS.gene62383.t1 MTR_1g077600 47.500 80 37 1 75 149 16 95 2.59e-21 92.8
MS.gene62383.t1 MTR_4g089095 69.643 56 16 1 97 152 16 70 2.69e-21 92.8
MS.gene62383.t1 MTR_1g077600 47.500 80 37 1 75 149 16 95 2.89e-21 93.2
MS.gene62383.t1 MTR_7g059400 53.521 71 31 1 82 150 59 129 3.02e-21 94.0
MS.gene62383.t1 MTR_8g015840 65.385 52 18 0 97 148 20 71 3.54e-21 91.3
MS.gene62383.t1 MTR_4g109980 64.151 53 19 0 95 147 38 90 5.91e-21 92.8
MS.gene62383.t1 MTR_7g024670 67.308 52 17 0 98 149 78 129 7.31e-21 93.2
MS.gene62383.t1 MTR_3g090430 54.167 72 31 1 81 150 33 104 3.05e-20 90.9
MS.gene62383.t1 MTR_5g031440 60.000 55 22 0 93 147 39 93 3.46e-20 90.9
MS.gene62383.t1 MTR_2g014170 67.347 49 16 0 97 145 45 93 8.30e-20 89.4
MS.gene62383.t1 MTR_3g077750 56.452 62 26 1 87 147 50 111 1.39e-19 89.4
MS.gene62383.t1 MTR_1g056810 65.385 52 18 0 99 150 14 65 1.63e-19 88.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62383.t1 AT3G47500 56.667 120 46 3 53 172 71 184 5.23e-39 145
MS.gene62383.t1 AT5G39660 44.828 174 61 6 63 221 92 245 1.18e-35 136
MS.gene62383.t1 AT5G39660 44.828 174 61 6 63 221 92 245 1.18e-35 136
MS.gene62383.t1 AT5G39660 44.828 174 61 6 63 221 92 245 1.18e-35 136
MS.gene62383.t1 AT5G62430 52.137 117 49 2 64 178 26 137 6.85e-35 130
MS.gene62383.t1 AT1G26790 48.951 143 60 4 55 184 67 209 9.06e-34 129
MS.gene62383.t1 AT2G34140 58.065 93 26 1 71 150 19 111 9.55e-34 124
MS.gene62383.t1 AT1G29160 78.333 60 13 0 91 150 56 115 3.80e-31 117
MS.gene62383.t1 AT1G69570 45.385 130 43 2 85 213 120 222 4.14e-31 122
MS.gene62383.t1 AT3G55370 55.714 70 31 0 93 162 72 141 5.35e-24 101
MS.gene62383.t1 AT3G55370 55.714 70 31 0 93 162 72 141 6.17e-24 102
MS.gene62383.t1 AT3G55370 55.714 70 31 0 93 162 117 186 1.29e-23 101
MS.gene62383.t1 AT2G28810 40.000 100 57 1 52 148 29 128 4.85e-23 99.0
MS.gene62383.t1 AT2G28810 40.000 100 57 1 52 148 46 145 5.83e-23 99.0
MS.gene62383.t1 AT5G60200 59.375 64 26 0 91 154 49 112 7.15e-23 97.1
MS.gene62383.t1 AT3G21270 69.811 53 16 0 97 149 29 81 1.09e-22 95.1
MS.gene62383.t1 AT2G37590 62.069 58 22 0 93 150 85 142 1.82e-22 97.4
MS.gene62383.t1 AT3G45610 45.055 91 42 2 93 175 36 126 3.66e-22 94.7
MS.gene62383.t1 AT1G51700 52.857 70 31 1 79 148 17 84 4.01e-22 93.2
MS.gene62383.t1 AT1G28310 64.286 56 20 0 97 152 27 82 1.28e-21 94.7
MS.gene62383.t1 AT4G24060 56.923 65 28 0 86 150 42 106 1.42e-21 95.1
MS.gene62383.t1 AT3G61850 61.017 59 23 0 92 150 57 115 1.42e-21 94.0
MS.gene62383.t1 AT3G61850 61.017 59 23 0 92 150 57 115 1.42e-21 94.0
MS.gene62383.t1 AT1G28310 64.286 56 20 0 97 152 51 106 1.43e-21 94.7
MS.gene62383.t1 AT3G61850 61.017 59 23 0 92 150 69 127 1.80e-21 94.0
MS.gene62383.t1 AT5G60850 66.667 54 18 0 97 150 51 104 1.91e-21 94.0
MS.gene62383.t1 AT3G61850 61.017 59 23 0 92 150 57 115 2.34e-21 94.0
MS.gene62383.t1 AT1G07640 66.038 53 18 0 97 149 21 73 2.57e-21 93.2
MS.gene62383.t1 AT2G28510 66.071 56 19 0 99 154 50 105 2.78e-21 93.2
MS.gene62383.t1 AT3G50410 66.038 53 18 0 97 149 30 82 2.78e-21 92.4
MS.gene62383.t1 AT3G61850 61.017 59 23 0 92 150 69 127 2.83e-21 94.0
MS.gene62383.t1 AT1G07640 66.038 53 18 0 97 149 77 129 3.81e-21 93.6
MS.gene62383.t1 AT5G02460 59.016 61 25 0 90 150 88 148 4.45e-21 94.4
MS.gene62383.t1 AT5G66940 53.425 73 27 1 84 156 26 91 4.74e-21 91.3
MS.gene62383.t1 AT1G07640 66.038 53 18 0 97 149 85 137 5.12e-21 93.2
MS.gene62383.t1 AT5G65590 64.815 54 19 0 97 150 41 94 6.27e-21 92.8
MS.gene62383.t1 AT4G00940 52.778 72 30 1 85 152 52 123 2.03e-20 90.9
MS.gene62383.t1 AT4G00940 52.778 72 30 1 85 152 52 123 2.03e-20 90.9
MS.gene62383.t1 AT4G00940 52.778 72 30 1 85 152 52 123 2.03e-20 90.9
MS.gene62383.t1 AT1G21340 42.553 94 53 1 99 191 40 133 3.60e-20 89.4
MS.gene62383.t1 AT5G62940 39.450 109 50 2 55 147 15 123 7.12e-20 90.5
MS.gene62383.t1 AT4G21050 47.436 78 39 1 77 152 2 79 7.75e-20 87.4
MS.gene62383.t1 AT4G38000 49.367 79 38 2 96 172 40 118 8.37e-20 88.2
MS.gene62383.t1 AT3G52440 63.462 52 19 0 99 150 27 78 2.11e-19 87.0
MS.gene62383.t1 AT1G64620 53.333 60 28 0 86 145 38 97 2.47e-19 88.6
MS.gene62383.t1 AT3G52440 63.462 52 19 0 99 150 46 97 3.33e-19 87.0
MS.gene62383.t1 AT1G47655 65.306 49 17 0 97 145 29 77 3.44e-19 85.5
MS.gene62383.t1 AT2G46590 58.491 53 22 0 92 144 63 115 1.34e-18 86.7
MS.gene62383.t1 AT2G46590 58.491 53 22 0 92 144 75 127 1.51e-18 86.7
MS.gene62383.t1 AT4G21040 47.222 72 38 0 99 170 27 98 2.39e-15 75.1
MS.gene62383.t1 AT4G21080 50.877 57 28 0 99 155 27 83 1.10e-14 73.6

Find 84 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGGTATTGTCCTAAAATT+TGG 0.197738 6.4:-2180848 MS.gene62383:CDS
CAATCTTGTCTGGTTTCTTT+AGG 0.212759 6.4:+2181170 None:intergenic
TAGAAACCAACGACTCTTCT+AGG 0.239536 6.4:-2180925 MS.gene62383:CDS
TTGGAACAATGTTACTTCTA+TGG 0.251370 6.4:-2180582 MS.gene62383:CDS
GGTAACAATTGTAGAGAAAA+TGG 0.277928 6.4:-2180746 MS.gene62383:CDS
GGTGAGTTCTTCTCAGATTC+AGG 0.283209 6.4:-2182309 MS.gene62383:CDS
AAGATTAAGCATTGAACCTT+TGG 0.298547 6.4:+2180799 None:intergenic
TCTTCTAGTTTCATGCTCTT+TGG 0.299226 6.4:-2182344 MS.gene62383:CDS
TTTAGGTGTTCAAATGATAA+TGG 0.318424 6.4:-2180866 MS.gene62383:CDS
GTCGGAGTTGTCACAGATAT+TGG 0.320960 6.4:-2181066 MS.gene62383:CDS
TTGTTCCAACTTTGATTCAT+TGG 0.340788 6.4:+2180596 None:intergenic
ATTTACCATGGTTGTAGAAT+TGG 0.341653 6.4:+2182286 None:intergenic
AAGCACTGTGGAAGATAATA+TGG 0.348391 6.4:-2181221 MS.gene62383:CDS
CCTTTGTATGGTAGTGGAAA+TGG 0.370217 6.4:-2180413 MS.gene62383:CDS
ATGCAGATGTGTAACCCTTA+TGG 0.380909 6.4:-2180548 MS.gene62383:CDS
AGAGTCGTTGGTTTCTAAAC+TGG 0.381811 6.4:+2180931 None:intergenic
TGCAGTAACTCCAAGCATAA+AGG 0.410347 6.4:-2181322 MS.gene62383:CDS
GGACATTGAACAATCTTGTC+TGG 0.411323 6.4:+2181160 None:intergenic
ACAAATTGCAAGCCAATGTT+TGG 0.418330 6.4:+2180461 None:intergenic
CATATCATTTCCTTTATGCT+TGG 0.421457 6.4:+2181312 None:intergenic
GCTCTTTGGTAAGAAGATTC+CGG 0.421687 6.4:-2182330 MS.gene62383:CDS
AGGTTTAGACCTATTCTGTT+CGG 0.445629 6.4:+2180652 None:intergenic
AATGCAACCATTTGAACCTA+TGG 0.446070 6.4:+2180382 None:intergenic
GTGTCACGCGCCGAGGGTTG+CGG 0.447216 6.4:-2180501 MS.gene62383:CDS
AGAGCTACCCTGCACCTTCA+TGG 0.447832 6.4:-2180625 MS.gene62383:CDS
ACTAGTAATAGTTGTATCTC+AGG 0.449615 6.4:-2180341 MS.gene62383:CDS
AATTTGTTCCTGCATCAAAC+AGG 0.461385 6.4:-2180445 MS.gene62383:CDS
AGAAATGTTGCTGTCGGTGC+AGG 0.467099 6.4:-2181025 MS.gene62383:CDS
GTTCCATTCCTGTTTGATGC+AGG 0.467432 6.4:+2180437 None:intergenic
TTCATTGGAATTATCCATGA+AGG 0.472286 6.4:+2180611 None:intergenic
TTGCATCAACATTTCTCTTG+TGG 0.474797 6.4:+2180771 None:intergenic
TATTGTCCTAAAATTTGGTC+GGG 0.484036 6.4:-2180843 MS.gene62383:CDS
TATTCGAATCCGAACAGAAT+AGG 0.487381 6.4:-2180661 MS.gene62383:CDS
GAAGTAGGATCAATGCCATA+AGG 0.489322 6.4:+2180533 None:intergenic
GGAATTATCCATGAAGGTGC+AGG 0.491213 6.4:+2180617 None:intergenic
CATGTGTGTTTGCCAAGGAT+TGG 0.493826 6.4:+2180308 None:intergenic
AATAGTTGTATCTCAGGTAA+TGG 0.494093 6.4:-2180335 MS.gene62383:CDS
GGAACACCTTTGTATGGTAG+TGG 0.497692 6.4:-2180419 MS.gene62383:CDS
TTGCTGTCGGTGCAGGGAGA+CGG 0.499466 6.4:-2181018 MS.gene62383:CDS
TAATTCCAATGAATCAAAGT+TGG 0.500347 6.4:-2180601 MS.gene62383:CDS
TGCAGTGGTGTCACGCGCCG+AGG 0.505833 6.4:-2180508 MS.gene62383:CDS
ATTTCTCATTGTTCCACCGT+TGG 0.510921 6.4:+2181042 None:intergenic
GCAGTGGTGTCACGCGCCGA+GGG 0.511659 6.4:-2180507 MS.gene62383:CDS
GGTAATGGCACACCAATCCT+TGG 0.524374 6.4:-2180320 MS.gene62383:CDS
AGGAATGGAACACCTTTGTA+TGG 0.533609 6.4:-2180425 MS.gene62383:CDS
TTGGTACCTAGAAGAGTCGT+TGG 0.535519 6.4:+2180919 None:intergenic
GTATTGTCCTAAAATTTGGT+CGG 0.547769 6.4:-2180844 MS.gene62383:CDS
ATTCAACATTTGAGCCATGT+TGG 0.548791 6.4:+2180900 None:intergenic
AATGTGTCCATAGGTTCAAA+TGG 0.549266 6.4:-2180389 MS.gene62383:CDS
GTTCCTGCATCAAACAGGAA+TGG 0.554691 6.4:-2180440 MS.gene62383:CDS
AAGAGAAATGTTGATGCAAG+TGG 0.555436 6.4:-2180767 MS.gene62383:CDS
TGTGTATCGTCTGTGACAAA+CGG 0.557160 6.4:-2180704 MS.gene62383:CDS
ACCTTTGGATTCATCGAGAG+AGG 0.559535 6.4:+2180814 None:intergenic
AAGTAGGATCAATGCCATAA+GGG 0.561604 6.4:+2180534 None:intergenic
GGAAATGGAAATGTGTCCAT+AGG 0.578755 6.4:-2180398 MS.gene62383:CDS
GATACAACTATTACTAGTAG+TGG 0.582605 6.4:+2180346 None:intergenic
TTGGTTTCTAAACTGGCGGT+CGG 0.586129 6.4:+2180938 None:intergenic
AATCTCATGTGTGTTTGCCA+AGG 0.586652 6.4:+2180303 None:intergenic
TGACACCACTGCATTGAAGT+AGG 0.587068 6.4:+2180518 None:intergenic
ATCAACCTCGGCATTTCTGT+CGG 0.588083 6.4:-2181084 MS.gene62383:CDS
ATGTTTGTAACCGCAACCCT+CGG 0.590311 6.4:+2180491 None:intergenic
TCAGGCCAATTCTACAACCA+TGG 0.595350 6.4:-2182291 MS.gene62383:intron
GGTGCAGGGTAGCTCTGCAT+AGG 0.600830 6.4:+2180632 None:intergenic
CACATGATTGTAATGTCCGA+TGG 0.606005 6.4:-2180965 MS.gene62383:CDS
GTCGTTGGTTTCTAAACTGG+CGG 0.606694 6.4:+2180934 None:intergenic
CCATTTCCACTACCATACAA+AGG 0.611279 6.4:+2180413 None:intergenic
CTCAAATGTTGAATCTGCTG+CGG 0.611992 6.4:-2180891 MS.gene62383:CDS
GCCTCTCTCGATGAATCCAA+AGG 0.612065 6.4:-2180815 MS.gene62383:CDS
TTACTAGTAGTGGATGAAGA+AGG 0.617817 6.4:+2180356 None:intergenic
CACAGATATTGGACCAACGG+TGG 0.629754 6.4:-2181055 MS.gene62383:CDS
TGAATCTGAGAAGAACTCAC+CGG 0.630634 6.4:+2182311 None:intergenic
GAATTATCCATGAAGGTGCA+GGG 0.632904 6.4:+2180618 None:intergenic
TGTCACAGATATTGGACCAA+CGG 0.638912 6.4:-2181058 MS.gene62383:CDS
ACTGGCGGTCGGAATTCCAT+CGG 0.649455 6.4:+2180949 None:intergenic
TTGATCCTACTTCAATGCAG+TGG 0.649462 6.4:-2180523 MS.gene62383:CDS
GTGACAAACGGTCATACTCG+AGG 0.662893 6.4:-2180692 MS.gene62383:CDS
GAAATGTTGCTGTCGGTGCA+GGG 0.663844 6.4:-2181024 MS.gene62383:CDS
CATCGGACATTACAATCATG+TGG 0.675212 6.4:+2180966 None:intergenic
ACTACAATGTCAATCAACCT+CGG 0.680110 6.4:-2181096 MS.gene62383:CDS
CAACTCCGACAGAAATGCCG+AGG 0.680790 6.4:+2181079 None:intergenic
GACTCTTCTAGGTACCAACA+TGG 0.687547 6.4:-2180914 MS.gene62383:CDS
ACAATGAGAAATGTTGCTGT+CGG 0.691886 6.4:-2181031 MS.gene62383:CDS
CAATTGTAGAGAAAATGGAG+AGG 0.705347 6.4:-2180741 MS.gene62383:CDS
AAAAGTGAACACAAGCACTG+TGG 0.727270 6.4:-2181233 MS.gene62383:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAGTTTTTTTTTTTGTTT+TGG - chr6.4:2180678-2180697 MS.gene62383:CDS 10.0%
!!! ATTTTTTATTTTTGTTGTAT+TGG - chr6.4:2180496-2180515 MS.gene62383:CDS 10.0%
!!! TCATTAAATTTGTTTAATTT+TGG - chr6.4:2180895-2180914 MS.gene62383:CDS 10.0%
!!! TTGATTTTTTATAAGAAAAT+TGG - chr6.4:2180803-2180822 MS.gene62383:CDS 10.0%
!!! TTTTTTATTTTTGTTGTATT+GGG - chr6.4:2180497-2180516 MS.gene62383:CDS 10.0%
!! AATTTAAATGTTTCAAAGAT+TGG + chr6.4:2180538-2180557 None:intergenic 15.0%
!! TAAAAAAAAACTCAAGAATA+TGG - chr6.4:2180602-2180621 MS.gene62383:CDS 15.0%
!! TTTCATAAATATAACATGAT+TGG - chr6.4:2180979-2180998 MS.gene62383:CDS 15.0%
!!! TAAATTTGTTTAATTTTGGT+TGG - chr6.4:2180899-2180918 MS.gene62383:CDS 15.0%
!! AAAAACCATAAAATTTGATG+TGG + chr6.4:2181226-2181245 None:intergenic 20.0%
!! AATTTAAGAAATTGAGTCTA+AGG - chr6.4:2180753-2180772 MS.gene62383:CDS 20.0%
!! ATGTCAATACATACATATAT+AGG - chr6.4:2180728-2180747 MS.gene62383:CDS 20.0%
!! TAAAAAAACGACATAAACAA+TGG + chr6.4:2180459-2180478 None:intergenic 20.0%
!!! TTAAGAGTATTTGATCTATA+TGG - chr6.4:2180559-2180578 MS.gene62383:CDS 20.0%
! AAGTCATGAAGAATAAAGTT+TGG + chr6.4:2181306-2181325 None:intergenic 25.0%
! AATGGTATTGTCCTAAAATT+TGG - chr6.4:2181836-2181855 MS.gene62383:intron 25.0%
! GATAAAATGAGTATTTACCA+TGG + chr6.4:2180413-2180432 None:intergenic 25.0%
! TAATTCCAATGAATCAAAGT+TGG - chr6.4:2182083-2182102 MS.gene62383:intron 25.0%
! TGTGAAATAAACTGAAATTG+TGG - chr6.4:2181011-2181030 MS.gene62383:CDS 25.0%
! TTTAGGTGTTCAAATGATAA+TGG - chr6.4:2181818-2181837 MS.gene62383:intron 25.0%
!! ATGTACCACATCAAATTTTA+TGG - chr6.4:2181218-2181237 MS.gene62383:CDS 25.0%
!!! CAGATTAGTTTTAGTAACAT+AGG - chr6.4:2181034-2181053 MS.gene62383:CDS 25.0%
!!! TTATTTTTGTTGTATTGGGT+TGG - chr6.4:2180501-2180520 MS.gene62383:CDS 25.0%
AATAGTTGTATCTCAGGTAA+TGG - chr6.4:2182349-2182368 MS.gene62383:CDS 30.0%
ACGACATAAACAATGGTTAT+TGG + chr6.4:2180452-2180471 None:intergenic 30.0%
ACTAGTAATAGTTGTATCTC+AGG - chr6.4:2182343-2182362 MS.gene62383:CDS 30.0%
ATTTACCATGGTTGTAGAAT+TGG + chr6.4:2180401-2180420 None:intergenic 30.0%
CATATCATTTCCTTTATGCT+TGG + chr6.4:2181375-2181394 None:intergenic 30.0%
CGACATAAACAATGGTTATT+GGG + chr6.4:2180451-2180470 None:intergenic 30.0%
GATACAACTATTACTAGTAG+TGG + chr6.4:2182341-2182360 None:intergenic 30.0%
GATCTGATTCATGTAAACAA+TGG - chr6.4:2180624-2180643 MS.gene62383:CDS 30.0%
GGTAACAATTGTAGAGAAAA+TGG - chr6.4:2181938-2181957 MS.gene62383:intron 30.0%
GTATTGTCCTAAAATTTGGT+CGG - chr6.4:2181840-2181859 MS.gene62383:intron 30.0%
TATTGTCCTAAAATTTGGTC+GGG - chr6.4:2181841-2181860 MS.gene62383:intron 30.0%
TTCATTGGAATTATCCATGA+AGG + chr6.4:2182076-2182095 None:intergenic 30.0%
TTGTTCCAACTTTGATTCAT+TGG + chr6.4:2182091-2182110 None:intergenic 30.0%
! AACATTTTCACACCAAACAT+TGG - chr6.4:2182211-2182230 MS.gene62383:intron 30.0%
! TTGGAACAATGTTACTTCTA+TGG - chr6.4:2182102-2182121 MS.gene62383:intron 30.0%
!! AAGATTAAGCATTGAACCTT+TGG + chr6.4:2181888-2181907 None:intergenic 30.0%
!! TTCTCAATGTTTTGAGTATG+AGG + chr6.4:2181398-2181417 None:intergenic 30.0%
AAGAGAAATGTTGATGCAAG+TGG - chr6.4:2181917-2181936 MS.gene62383:intron 35.0%
AAGTAGGATCAATGCCATAA+GGG + chr6.4:2182153-2182172 None:intergenic 35.0%
AATGCAACCATTTGAACCTA+TGG + chr6.4:2182305-2182324 None:intergenic 35.0%
AATGTGTCCATAGGTTCAAA+TGG - chr6.4:2182295-2182314 MS.gene62383:CDS 35.0%
ACAAATTGCAAGCCAATGTT+TGG + chr6.4:2182226-2182245 None:intergenic 35.0%
ACAATGAGAAATGTTGCTGT+CGG - chr6.4:2181653-2181672 MS.gene62383:intron 35.0%
ACTACAATGTCAATCAACCT+CGG - chr6.4:2181588-2181607 MS.gene62383:intron 35.0%
AGGTTTAGACCTATTCTGTT+CGG + chr6.4:2182035-2182054 None:intergenic 35.0%
ATTCAACATTTGAGCCATGT+TGG + chr6.4:2181787-2181806 None:intergenic 35.0%
CAATTGTAGAGAAAATGGAG+AGG - chr6.4:2181943-2181962 MS.gene62383:intron 35.0%
TATTCGAATCCGAACAGAAT+AGG - chr6.4:2182023-2182042 MS.gene62383:intron 35.0%
TGATTCATGTAAACAATGGC+TGG - chr6.4:2180628-2180647 MS.gene62383:CDS 35.0%
TTGCATCAACATTTCTCTTG+TGG + chr6.4:2181916-2181935 None:intergenic 35.0%
! AAGCACTGTGGAAGATAATA+TGG - chr6.4:2181463-2181482 MS.gene62383:intron 35.0%
! AATTTGTTCCTGCATCAAAC+AGG - chr6.4:2182239-2182258 MS.gene62383:intron 35.0%
! CAATCTTGTCTGGTTTCTTT+AGG + chr6.4:2181517-2181536 None:intergenic 35.0%
! CATTTTCCCGACCAAATTTT+AGG + chr6.4:2181850-2181869 None:intergenic 35.0%
!! TCTTCTAGTTTCATGCTCTT+TGG - chr6.4:2180340-2180359 MS.gene62383:CDS 35.0%
!! TTACTAGTAGTGGATGAAGA+AGG + chr6.4:2182331-2182350 None:intergenic 35.0%
AGGAATGGAACACCTTTGTA+TGG - chr6.4:2182259-2182278 MS.gene62383:intron 40.0%
ATGCAGATGTGTAACCCTTA+TGG - chr6.4:2182136-2182155 MS.gene62383:intron 40.0%
ATTTCTCATTGTTCCACCGT+TGG + chr6.4:2181645-2181664 None:intergenic 40.0%
CACATGATTGTAATGTCCGA+TGG - chr6.4:2181719-2181738 MS.gene62383:intron 40.0%
CATCGGACATTACAATCATG+TGG + chr6.4:2181721-2181740 None:intergenic 40.0%
CCATTTCCACTACCATACAA+AGG + chr6.4:2182274-2182293 None:intergenic 40.0%
CGCTGAGTTTGACAAAATCA+CGG - chr6.4:2181086-2181105 MS.gene62383:CDS 40.0%
CTCAAATGTTGAATCTGCTG+CGG - chr6.4:2181793-2181812 MS.gene62383:intron 40.0%
CTGGTAATTCCATAAACCAG+GGG + chr6.4:2181066-2181085 None:intergenic 40.0%
GAAGTAGGATCAATGCCATA+AGG + chr6.4:2182154-2182173 None:intergenic 40.0%
GCTCTTTGGTAAGAAGATTC+CGG - chr6.4:2180354-2180373 MS.gene62383:CDS 40.0%
GCTGGTAATTCCATAAACCA+GGG + chr6.4:2181067-2181086 None:intergenic 40.0%
GGAAATGGAAATGTGTCCAT+AGG - chr6.4:2182286-2182305 MS.gene62383:intron 40.0%
GGACATTGAACAATCTTGTC+TGG + chr6.4:2181527-2181546 None:intergenic 40.0%
TAGAAACCAACGACTCTTCT+AGG - chr6.4:2181759-2181778 MS.gene62383:intron 40.0%
TGAATCTGAGAAGAACTCAC+CGG + chr6.4:2180376-2180395 None:intergenic 40.0%
TGCAGTAACTCCAAGCATAA+AGG - chr6.4:2181362-2181381 MS.gene62383:intron 40.0%
TGTCACAGATATTGGACCAA+CGG - chr6.4:2181626-2181645 MS.gene62383:intron 40.0%
TGTGTATCGTCTGTGACAAA+CGG - chr6.4:2181980-2181999 MS.gene62383:intron 40.0%
TTGATCCTACTTCAATGCAG+TGG - chr6.4:2182161-2182180 MS.gene62383:intron 40.0%
! AAAAGTGAACACAAGCACTG+TGG - chr6.4:2181451-2181470 MS.gene62383:intron 40.0%
! GAATTATCCATGAAGGTGCA+GGG + chr6.4:2182069-2182088 None:intergenic 40.0%
! GTGTCGAAACTTTTGCATCT+CGG + chr6.4:2181548-2181567 None:intergenic 40.0%
! TTGAATCTGCTGCGGTTTTT+AGG - chr6.4:2181801-2181820 MS.gene62383:intron 40.0%
!! AGAGTCGTTGGTTTCTAAAC+TGG + chr6.4:2181756-2181775 None:intergenic 40.0%
!! CCTTTGTATGGTAGTGGAAA+TGG - chr6.4:2182271-2182290 MS.gene62383:intron 40.0%
ACCTTTGGATTCATCGAGAG+AGG + chr6.4:2181873-2181892 None:intergenic 45.0%
ATCAACCTCGGCATTTCTGT+CGG - chr6.4:2181600-2181619 MS.gene62383:intron 45.0%
ATGTTTGTAACCGCAACCCT+CGG + chr6.4:2182196-2182215 None:intergenic 45.0%
CATGTGTGTTTGCCAAGGAT+TGG + chr6.4:2182379-2182398 None:intergenic 45.0%
CGCTGGTAATTCCATAAACC+AGG + chr6.4:2181068-2181087 None:intergenic 45.0%
GACTCTTCTAGGTACCAACA+TGG - chr6.4:2181770-2181789 MS.gene62383:intron 45.0%
GGAACACCTTTGTATGGTAG+TGG - chr6.4:2182265-2182284 MS.gene62383:intron 45.0%
GGTGAGTTCTTCTCAGATTC+AGG - chr6.4:2180375-2180394 MS.gene62383:CDS 45.0%
GTCGGAGTTGTCACAGATAT+TGG - chr6.4:2181618-2181637 MS.gene62383:intron 45.0%
GTTCCATTCCTGTTTGATGC+AGG + chr6.4:2182250-2182269 None:intergenic 45.0%
GTTCCTGCATCAAACAGGAA+TGG - chr6.4:2182244-2182263 MS.gene62383:intron 45.0%
TCAGGCCAATTCTACAACCA+TGG - chr6.4:2180393-2180412 MS.gene62383:CDS 45.0%
TGACACCACTGCATTGAAGT+AGG + chr6.4:2182169-2182188 None:intergenic 45.0%
TTGGTACCTAGAAGAGTCGT+TGG + chr6.4:2181768-2181787 None:intergenic 45.0%
TTGGTTTCTAAACTGGCGGT+CGG + chr6.4:2181749-2181768 None:intergenic 45.0%
! GATTTTGTCAAACTCAGCGC+TGG + chr6.4:2181085-2181104 None:intergenic 45.0%
! GGAATTATCCATGAAGGTGC+AGG + chr6.4:2182070-2182089 None:intergenic 45.0%
!! GTCGTTGGTTTCTAAACTGG+CGG + chr6.4:2181753-2181772 None:intergenic 45.0%
AGAAATGTTGCTGTCGGTGC+AGG - chr6.4:2181659-2181678 MS.gene62383:intron 50.0%
AGGAGATAGCCCCTGGTTTA+TGG - chr6.4:2181054-2181073 MS.gene62383:CDS 50.0%
CACAGATATTGGACCAACGG+TGG - chr6.4:2181629-2181648 MS.gene62383:intron 50.0%
GAAATGTTGCTGTCGGTGCA+GGG - chr6.4:2181660-2181679 MS.gene62383:intron 50.0%
GCCTCTCTCGATGAATCCAA+AGG - chr6.4:2181869-2181888 MS.gene62383:intron 50.0%
GTAACATAGGAGATAGCCCC+TGG - chr6.4:2181047-2181066 MS.gene62383:CDS 50.0%
GTGACAAACGGTCATACTCG+AGG - chr6.4:2181992-2182011 MS.gene62383:intron 50.0%
! GGTAATGGCACACCAATCCT+TGG - chr6.4:2182364-2182383 MS.gene62383:CDS 50.0%
ACTGGCGGTCGGAATTCCAT+CGG + chr6.4:2181738-2181757 None:intergenic 55.0%
AGAGCTACCCTGCACCTTCA+TGG - chr6.4:2182059-2182078 MS.gene62383:intron 55.0%
CAACTCCGACAGAAATGCCG+AGG + chr6.4:2181608-2181627 None:intergenic 55.0%
TTGCTGTCGGTGCAGGGAGA+CGG - chr6.4:2181666-2181685 MS.gene62383:intron 60.0%
! GGTGCAGGGTAGCTCTGCAT+AGG + chr6.4:2182055-2182074 None:intergenic 60.0%
GTGTCACGCGCCGAGGGTTG+CGG - chr6.4:2182183-2182202 MS.gene62383:intron 70.0%
!! GCAGTGGTGTCACGCGCCGA+GGG - chr6.4:2182177-2182196 MS.gene62383:intron 70.0%
!! TGCAGTGGTGTCACGCGCCG+AGG - chr6.4:2182176-2182195 MS.gene62383:intron 70.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 2180307 2182399 2180307 ID=MS.gene62383
chr6.4 mRNA 2180307 2182399 2180307 ID=MS.gene62383.t1;Parent=MS.gene62383
chr6.4 exon 2182292 2182399 2182292 ID=MS.gene62383.t1.exon1;Parent=MS.gene62383.t1
chr6.4 CDS 2182292 2182399 2182292 ID=cds.MS.gene62383.t1;Parent=MS.gene62383.t1
chr6.4 exon 2180307 2181350 2180307 ID=MS.gene62383.t1.exon2;Parent=MS.gene62383.t1
chr6.4 CDS 2180307 2181350 2180307 ID=cds.MS.gene62383.t1;Parent=MS.gene62383.t1
Gene Sequence

>MS.gene62383

ATGAATGGTGAAGGTGACATGTTTGTTAAAGATTCTTCTAGTTTCATGCTCTTTGGTAAGAAGATTCCGGTGAGTTCTTCTCAGATTCAGGCCAATTCTACAACCATGGTAAATACTCATTTTATCATATTATATCATATACCCAATAACCATTGTTTATGTCGTTTTTTTATTTGAAAAAATTTAAATATTTTTTATTTTTGTTGTATTGGGTTGGTATAATATTTTCCAATCTTTGAAACATTTAAATTTTTAAGAGTATTTGATCTATATGGTTTCTTTTTTAATATATTGATAAAAAAAAACTCAAGAATATGGATCTGATTCATGTAAACAATGGCTGGTCTTAATTGTATGAATTTTAATAAAAAAAAAGTTTTTTTTTTTGTTTTGGTAAAATTATAGTTGAGAATTTATGTTTATGTCAATACATACATATATAGGTGAATTTAAGAAATTGAGTCTAAGGTGTTGTTGAGCTGTCTTGTGCAAGTAGTTGATTTTTTATAAGAAAATTGGATCTGTTGCATTTTTAATGATACTTATTTATTTTTTGTGAAATTGAGTTGTACTTTTGAAAGAAATTTATCATTAAATTTGTTTAATTTTGGTTGGAGTATTAATCAAATCTTAAGAAAGTTCAATGTTTAATCAATTACAAATAGATTTGTTTTTCATAAATATAACATGATTGGTTGAATGAATGTGAAATAAACTGAAATTGTGGCAGATTAGTTTTAGTAACATAGGAGATAGCCCCTGGTTTATGGAATTACCAGCGCTGAGTTTGACAAAATCACGGTGACATGTTTGTTGAAGCTCGAAAGAGTAGATTTTTCTAAATTACAGTGACACGTGATTATGTCAAACTCATCGTCAATCTAAACATACGAAATGAAAGTTCTTATGTTATGTACCACATCAAATTTTATGGTTTTTTATGTGAAAAATGAATATAACTTTGATTTTGTTTCTTGTTTATTGTCAAATTTGTTGCCAAACTTTATTCTTCATGACTTGTCACTGATTATATAGTTTTTATTTTACAGATTTCTTGCAGTAACTCCAAGCATAAAGGAAATGATATGCCTCATACTCAAAACATTGAGAAATCAAAGAATTCTTCTAATGAAGAAAATGAATCAAAAGTGAACACAAGCACTGTGGAAGATAATATGGAAACTAGCAGCACAGATCAAGACAAAGTCCTAAAGAAACCAGACAAGATTGTTCAATGTCCGAGATGCAAAAGTTTCGACACAAAATTTTGTTATTTCAACAACTACAATGTCAATCAACCTCGGCATTTCTGTCGGAGTTGTCACAGATATTGGACCAACGGTGGAACAATGAGAAATGTTGCTGTCGGTGCAGGGAGACGGAAGAACAAACACATAGCTTCTCAATATCGCCACATGATTGTAATGTCCGATGGAATTCCGACCGCCAGTTTAGAAACCAACGACTCTTCTAGGTACCAACATGGCTCAAATGTTGAATCTGCTGCGGTTTTTAGGTGTTCAAATGATAATGGTATTGTCCTAAAATTTGGTCGGGAAAATGCCTCTCTCGATGAATCCAAAGGTTCAATGCTTAATCTTATGAACCACAAGAGAAATGTTGATGCAAGTGGTAACAATTGTAGAGAAAATGGAGAGGAAGAATCATCGTTATGTGTATCGTCTGTGACAAACGGTCATACTCGAGGAAATGAGTTATTCGAATCCGAACAGAATAGGTCTAAACCTATGCAGAGCTACCCTGCACCTTCATGGATAATTCCAATGAATCAAAGTTGGAACAATGTTACTTCTATGGTTCAATCATCAATGCAGATGTGTAACCCTTATGGCATTGATCCTACTTCAATGCAGTGGTGTCACGCGCCGAGGGTTGCGGTTACAAACATTTTCACACCAAACATTGGCTTGCAATTTGTTCCTGCATCAAACAGGAATGGAACACCTTTGTATGGTAGTGGAAATGGAAATGTGTCCATAGGTTCAAATGGTTGCATTTCACCTTCTTCATCCACTACTAGTAATAGTTGTATCTCAGGTAATGGCACACCAATCCTTGGCAAACACACATGA
Protein sequence

>MS.gene62383.t1

MNGEGDMFVKDSSSFMLFGKKIPVSSSQIQANSTTMISCSNSKHKGNDMPHTQNIEKSKNSSNEENESKVNTSTVEDNMETSSTDQDKVLKKPDKIVQCPRCKSFDTKFCYFNNYNVNQPRHFCRSCHRYWTNGGTMRNVAVGAGRRKNKHIASQYRHMIVMSDGIPTASLETNDSSRYQHGSNVESAAVFRCSNDNGIVLKFGRENASLDESKGSMLNLMNHKRNVDASGNNCRENGEEESSLCVSSVTNGHTRGNELFESEQNRSKPMQSYPAPSWIIPMNQSWNNVTSMVQSSMQMCNPYGIDPTSMQWCHAPRVAVTNIFTPNIGLQFVPASNRNGTPLYGSGNGNVSIGSNGCISPSSSTTSNSCISGNGTPILGKHT