Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62384.t1 | KAE8706705.1 | 55.6 | 63 | 27 | 1 | 112 | 174 | 231 | 292 | 2.00E-11 | 79.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62384.t1 | Q9LVW4 | 58.6 | 58 | 23 | 1 | 117 | 174 | 184 | 240 | 4.4e-12 | 72.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62384.t1 | A0A2T7EK85 | 52.0 | 75 | 28 | 2 | 107 | 174 | 111 | 184 | 3.1e-11 | 78.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene62384.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62384.t1 | MTR_4g088015 | 66.667 | 54 | 17 | 1 | 121 | 174 | 220 | 272 | 2.95e-17 | 78.6 |
| MS.gene62384.t1 | MTR_3g463400 | 57.407 | 54 | 22 | 1 | 121 | 174 | 132 | 184 | 5.24e-15 | 72.0 |
| MS.gene62384.t1 | MTR_5g070020 | 57.407 | 54 | 22 | 1 | 121 | 174 | 121 | 173 | 1.27e-14 | 70.9 |
| MS.gene62384.t1 | MTR_5g049190 | 52.542 | 59 | 27 | 1 | 116 | 174 | 13 | 70 | 1.70e-14 | 69.3 |
| MS.gene62384.t1 | MTR_7g451170 | 57.407 | 54 | 22 | 1 | 121 | 174 | 190 | 242 | 2.59e-14 | 70.1 |
| MS.gene62384.t1 | MTR_7g037260 | 55.556 | 54 | 23 | 1 | 121 | 174 | 144 | 196 | 2.92e-13 | 67.0 |
| MS.gene62384.t1 | MTR_7g035350 | 52.727 | 55 | 25 | 1 | 120 | 174 | 223 | 276 | 3.14e-13 | 67.0 |
| MS.gene62384.t1 | MTR_3g077650 | 55.556 | 54 | 23 | 1 | 121 | 174 | 180 | 232 | 4.60e-13 | 66.6 |
| MS.gene62384.t1 | MTR_1g057980 | 56.364 | 55 | 22 | 2 | 121 | 174 | 78 | 131 | 7.67e-13 | 65.5 |
| MS.gene62384.t1 | MTR_4g105660 | 40.594 | 101 | 43 | 4 | 86 | 177 | 142 | 234 | 8.93e-13 | 65.9 |
| MS.gene62384.t1 | MTR_7g037130 | 51.852 | 54 | 25 | 1 | 121 | 174 | 144 | 196 | 3.25e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62384.t1 | AT3G27785 | 58.621 | 58 | 23 | 1 | 117 | 174 | 184 | 240 | 1.39e-15 | 73.9 |
| MS.gene62384.t1 | AT5G58850 | 53.704 | 54 | 24 | 1 | 121 | 174 | 104 | 156 | 3.66e-14 | 69.7 |
| MS.gene62384.t1 | AT5G11050 | 55.556 | 54 | 23 | 1 | 121 | 174 | 104 | 156 | 3.99e-14 | 69.7 |
| MS.gene62384.t1 | AT4G18770 | 47.541 | 61 | 31 | 1 | 114 | 174 | 209 | 268 | 1.34e-13 | 68.2 |
| MS.gene62384.t1 | AT5G40360 | 31.111 | 135 | 65 | 4 | 67 | 174 | 76 | 209 | 5.90e-12 | 63.2 |
Find 36 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATATAGGGAACTTGTTAAAT+TGG | 0.199609 | 6.4:-2192937 | MS.gene62384:intron |
| TAGACGACTGTTTAAGATAT+TGG | 0.218716 | 6.4:+2193344 | None:intergenic |
| CGCTCCAAACATGGATATTC+TGG | 0.220757 | 6.4:+2193483 | None:intergenic |
| TTTAAGATATTGGGATTATT+AGG | 0.225450 | 6.4:+2193354 | None:intergenic |
| TCATCTCCGTCCAAACATTA+AGG | 0.270772 | 6.4:-2192823 | MS.gene62384:CDS |
| AGACGACTGTTTAAGATATT+GGG | 0.328045 | 6.4:+2193345 | None:intergenic |
| ATGATGACATGCCACATAAA+TGG | 0.337358 | 6.4:+2193301 | None:intergenic |
| GGAACATAAACCTTAATGTT+TGG | 0.342814 | 6.4:+2192813 | None:intergenic |
| TTCATCAATCTTGCAATTTG+AGG | 0.352946 | 6.4:+2192888 | None:intergenic |
| GCATAAGCTTTGTTGACGTC+TGG | 0.422118 | 6.4:+2193201 | None:intergenic |
| AAATGGATGACATGCATGTT+TGG | 0.422300 | 6.4:+2193318 | None:intergenic |
| CAAAACAGTGTCGTGATAGA+TGG | 0.437524 | 6.4:-2192851 | MS.gene62384:CDS |
| GAACGAGAATTGACCTTTGT+GGG | 0.444047 | 6.4:+2193448 | None:intergenic |
| TTGTTGACGTCTGGTACAAA+TGG | 0.468340 | 6.4:+2193210 | None:intergenic |
| TATTAGGTACACTCAAGATA+TGG | 0.470686 | 6.4:+2193370 | None:intergenic |
| CTTATGCAATCAGAAAGAAA+TGG | 0.496059 | 6.4:-2193185 | MS.gene62384:CDS |
| CATAAACCTTAATGTTTGGA+CGG | 0.508200 | 6.4:+2192817 | None:intergenic |
| ACCATCTCGATGGAAACATC+TGG | 0.520062 | 6.4:+2193245 | None:intergenic |
| GTTAAATTGGTGAATCAACA+TGG | 0.522743 | 6.4:-2192924 | MS.gene62384:CDS |
| AAGGGATTGTTCTGAGGAGG+TGG | 0.523100 | 6.4:+2193426 | None:intergenic |
| GGAACGAGAATTGACCTTTG+TGG | 0.525598 | 6.4:+2193447 | None:intergenic |
| TCGAAAGATTGAGAAATGAC+AGG | 0.547098 | 6.4:+2193270 | None:intergenic |
| TGAATCACACGCTCCAAACA+TGG | 0.559827 | 6.4:+2193474 | None:intergenic |
| AACAAGGGATTGTTCTGAGG+AGG | 0.564533 | 6.4:+2193423 | None:intergenic |
| ATGCATGTCATCCATTTATG+TGG | 0.566202 | 6.4:-2193312 | MS.gene62384:CDS |
| TTGAGATGGTAGTTTAACAA+GGG | 0.576755 | 6.4:+2193408 | None:intergenic |
| TTTAACAAGGGATTGTTCTG+AGG | 0.590649 | 6.4:+2193420 | None:intergenic |
| CATGTAAATTAGTGTTGAGA+TGG | 0.594522 | 6.4:+2193394 | None:intergenic |
| ACCAGATGTTTCCATCGAGA+TGG | 0.598386 | 6.4:-2193246 | MS.gene62384:CDS |
| GTTGAGATGGTAGTTTAACA+AGG | 0.598487 | 6.4:+2193407 | None:intergenic |
| TGTTAAGAAGACCATCTCGA+TGG | 0.624376 | 6.4:+2193235 | None:intergenic |
| AAAGAAATGGACAACCAATG+AGG | 0.657128 | 6.4:-2193172 | MS.gene62384:CDS |
| TTAAATTGGTGAATCAACAT+GGG | 0.666593 | 6.4:-2192923 | MS.gene62384:CDS |
| ATTGCAAGATTGATGAAGCG+AGG | 0.667312 | 6.4:-2192882 | MS.gene62384:CDS |
| AACGAGAATTGACCTTTGTG+GGG | 0.672668 | 6.4:+2193449 | None:intergenic |
| GTGTGATTCATACCCCACAA+AGG | 0.701235 | 6.4:-2193461 | MS.gene62384:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAATTGTGATTTCATATA+GGG | - | chr6.4:2193394-2193413 | MS.gene62384:CDS | 15.0% |
| !!! | AGGTTTTTTTAATGTTAAAA+TGG | - | chr6.4:2193356-2193375 | MS.gene62384:CDS | 15.0% |
| !!! | GGTTTTTTTAATGTTAAAAT+GGG | - | chr6.4:2193357-2193376 | MS.gene62384:CDS | 15.0% |
| !!! | GTTTTTTTAATGTTAAAATG+GGG | - | chr6.4:2193358-2193377 | MS.gene62384:CDS | 15.0% |
| !! | AACCTTATATTGATGAAAAA+TGG | + | chr6.4:2193341-2193360 | None:intergenic | 20.0% |
| !! | CTTAAATTGTGATTTCATAT+AGG | - | chr6.4:2193393-2193412 | MS.gene62384:CDS | 20.0% |
| !! | TATAGAAAAAATCCAAAGAA+AGG | + | chr6.4:2193252-2193271 | None:intergenic | 20.0% |
| !!! | TACCATTTTTCATCAATATA+AGG | - | chr6.4:2193336-2193355 | MS.gene62384:CDS | 20.0% |
| !!! | TTTAAGATATTGGGATTATT+AGG | + | chr6.4:2192995-2193014 | None:intergenic | 20.0% |
| !!! | TTTATCTTTTTTCCTTTCTT+TGG | - | chr6.4:2193237-2193256 | MS.gene62384:CDS | 20.0% |
| ! | TTAAATTGGTGAATCAACAT+GGG | - | chr6.4:2193423-2193442 | MS.gene62384:CDS | 25.0% |
| !! | ATTCTGGAAAAAGATTCAAA+AGG | + | chr6.4:2192850-2192869 | None:intergenic | 25.0% |
| !! | TTTTACATACATATCCTCAT+TGG | + | chr6.4:2193191-2193210 | None:intergenic | 25.0% |
| !!! | ATATAGGGAACTTGTTAAAT+TGG | - | chr6.4:2193409-2193428 | MS.gene62384:CDS | 25.0% |
| CATAAACCTTAATGTTTGGA+CGG | + | chr6.4:2193532-2193551 | None:intergenic | 30.0% | |
| CTTATGCAATCAGAAAGAAA+TGG | - | chr6.4:2193161-2193180 | MS.gene62384:intron | 30.0% | |
| GGAACATAAACCTTAATGTT+TGG | + | chr6.4:2193536-2193555 | None:intergenic | 30.0% | |
| GTTAAATTGGTGAATCAACA+TGG | - | chr6.4:2193422-2193441 | MS.gene62384:CDS | 30.0% | |
| TATTAGGTACACTCAAGATA+TGG | + | chr6.4:2192979-2192998 | None:intergenic | 30.0% | |
| TTCATCAATCTTGCAATTTG+AGG | + | chr6.4:2193461-2193480 | None:intergenic | 30.0% | |
| TTGAGATGGTAGTTTAACAA+GGG | + | chr6.4:2192941-2192960 | None:intergenic | 30.0% | |
| ! | AGACGACTGTTTAAGATATT+GGG | + | chr6.4:2193004-2193023 | None:intergenic | 30.0% |
| ! | CATGTAAATTAGTGTTGAGA+TGG | + | chr6.4:2192955-2192974 | None:intergenic | 30.0% |
| ! | TAGACGACTGTTTAAGATAT+TGG | + | chr6.4:2193005-2193024 | None:intergenic | 30.0% |
| ! | TTTTCCAGAATATCCATGTT+TGG | - | chr6.4:2192859-2192878 | MS.gene62384:CDS | 30.0% |
| AAAGAAATGGACAACCAATG+AGG | - | chr6.4:2193174-2193193 | MS.gene62384:CDS | 35.0% | |
| AAATGGATGACATGCATGTT+TGG | + | chr6.4:2193031-2193050 | None:intergenic | 35.0% | |
| ATGATGACATGCCACATAAA+TGG | + | chr6.4:2193048-2193067 | None:intergenic | 35.0% | |
| ATGCATGTCATCCATTTATG+TGG | - | chr6.4:2193034-2193053 | MS.gene62384:intron | 35.0% | |
| CTACCAATAATTGAACAGTG+CGG | + | chr6.4:2193307-2193326 | None:intergenic | 35.0% | |
| GTTGAGATGGTAGTTTAACA+AGG | + | chr6.4:2192942-2192961 | None:intergenic | 35.0% | |
| TAACCGCACTGTTCAATTAT+TGG | - | chr6.4:2193301-2193320 | MS.gene62384:CDS | 35.0% | |
| TCGAAAGATTGAGAAATGAC+AGG | + | chr6.4:2193079-2193098 | None:intergenic | 35.0% | |
| TTTAACAAGGGATTGTTCTG+AGG | + | chr6.4:2192929-2192948 | None:intergenic | 35.0% | |
| ! | ATCAACATGGGCTGAAAAAA+TGG | - | chr6.4:2193435-2193454 | MS.gene62384:CDS | 35.0% |
| AACGAGAATTGACCTTTGTG+GGG | + | chr6.4:2192900-2192919 | None:intergenic | 40.0% | |
| ATTGCAAGATTGATGAAGCG+AGG | - | chr6.4:2193464-2193483 | MS.gene62384:CDS | 40.0% | |
| CAAAACAGTGTCGTGATAGA+TGG | - | chr6.4:2193495-2193514 | MS.gene62384:CDS | 40.0% | |
| GAACGAGAATTGACCTTTGT+GGG | + | chr6.4:2192901-2192920 | None:intergenic | 40.0% | |
| TCATCTCCGTCCAAACATTA+AGG | - | chr6.4:2193523-2193542 | MS.gene62384:CDS | 40.0% | |
| TGTTAAGAAGACCATCTCGA+TGG | + | chr6.4:2193114-2193133 | None:intergenic | 40.0% | |
| TTGTTGACGTCTGGTACAAA+TGG | + | chr6.4:2193139-2193158 | None:intergenic | 40.0% | |
| ACCAGATGTTTCCATCGAGA+TGG | - | chr6.4:2193100-2193119 | MS.gene62384:intron | 45.0% | |
| ACCATCTCGATGGAAACATC+TGG | + | chr6.4:2193104-2193123 | None:intergenic | 45.0% | |
| CGCTCCAAACATGGATATTC+TGG | + | chr6.4:2192866-2192885 | None:intergenic | 45.0% | |
| GGAACGAGAATTGACCTTTG+TGG | + | chr6.4:2192902-2192921 | None:intergenic | 45.0% | |
| GTGTGATTCATACCCCACAA+AGG | - | chr6.4:2192885-2192904 | MS.gene62384:CDS | 45.0% | |
| TGAATCACACGCTCCAAACA+TGG | + | chr6.4:2192875-2192894 | None:intergenic | 45.0% | |
| !! | AACAAGGGATTGTTCTGAGG+AGG | + | chr6.4:2192926-2192945 | None:intergenic | 45.0% |
| !! | GCATAAGCTTTGTTGACGTC+TGG | + | chr6.4:2193148-2193167 | None:intergenic | 45.0% |
| !! | AAGGGATTGTTCTGAGGAGG+TGG | + | chr6.4:2192923-2192942 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 2192809 | 2193559 | 2192809 | ID=MS.gene62384 |
| chr6.4 | mRNA | 2192809 | 2193559 | 2192809 | ID=MS.gene62384.t1;Parent=MS.gene62384 |
| chr6.4 | exon | 2193168 | 2193559 | 2193168 | ID=MS.gene62384.t1.exon1;Parent=MS.gene62384.t1 |
| chr6.4 | CDS | 2193168 | 2193559 | 2193168 | ID=cds.MS.gene62384.t1;Parent=MS.gene62384.t1 |
| chr6.4 | exon | 2192809 | 2192953 | 2192809 | ID=MS.gene62384.t1.exon2;Parent=MS.gene62384.t1 |
| chr6.4 | CDS | 2192809 | 2192953 | 2192809 | ID=cds.MS.gene62384.t1;Parent=MS.gene62384.t1 |
| Gene Sequence |
| Protein sequence |
