Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62409.t1 | XP_024632961.1 | 83.1 | 249 | 39 | 1 | 1 | 249 | 81 | 326 | 6.80E-117 | 430.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62409.t1 | Q9SWG3 | 24.8 | 226 | 153 | 5 | 5 | 229 | 81 | 290 | 7.3e-11 | 69.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62409.t1 | A0A072TE13 | 87.8 | 229 | 28 | 0 | 1 | 229 | 66 | 294 | 2.3e-114 | 421.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene62409.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62409.t1 | MTR_0558s0020 | 87.391 | 230 | 29 | 0 | 1 | 230 | 66 | 295 | 8.07e-156 | 436 |
| MS.gene62409.t1 | MTR_6g090505 | 84.348 | 230 | 36 | 0 | 1 | 230 | 1 | 230 | 1.02e-146 | 415 |
| MS.gene62409.t1 | MTR_1g083640 | 88.725 | 204 | 23 | 0 | 1 | 204 | 81 | 284 | 1.68e-139 | 394 |
| MS.gene62409.t1 | MTR_8g446020 | 78.414 | 227 | 49 | 0 | 4 | 230 | 29 | 255 | 5.85e-134 | 379 |
| MS.gene62409.t1 | MTR_2g014100 | 86.047 | 172 | 24 | 0 | 1 | 172 | 74 | 245 | 3.17e-111 | 321 |
| MS.gene62409.t1 | MTR_0337s0040 | 77.487 | 191 | 43 | 0 | 4 | 194 | 30 | 220 | 5.62e-109 | 314 |
| MS.gene62409.t1 | MTR_1g055240 | 86.620 | 142 | 19 | 0 | 27 | 168 | 3 | 144 | 4.94e-90 | 263 |
| MS.gene62409.t1 | MTR_0019s0290 | 80.921 | 152 | 29 | 0 | 18 | 169 | 9 | 160 | 4.43e-89 | 261 |
| MS.gene62409.t1 | MTR_3g008240 | 86.232 | 138 | 19 | 0 | 1 | 138 | 66 | 203 | 6.29e-85 | 252 |
| MS.gene62409.t1 | MTR_4g040430 | 84.783 | 138 | 17 | 1 | 1 | 138 | 65 | 198 | 3.93e-82 | 245 |
| MS.gene62409.t1 | MTR_5g071590 | 80.583 | 103 | 20 | 0 | 1 | 103 | 78 | 180 | 9.98e-60 | 188 |
| MS.gene62409.t1 | MTR_1281s0010 | 80.952 | 63 | 12 | 0 | 1 | 63 | 47 | 109 | 5.38e-31 | 112 |
| MS.gene62409.t1 | MTR_6g051425 | 33.333 | 204 | 124 | 3 | 21 | 224 | 64 | 255 | 4.91e-30 | 115 |
| MS.gene62409.t1 | MTR_1g070355 | 27.053 | 207 | 137 | 2 | 21 | 227 | 119 | 311 | 4.12e-17 | 81.3 |
| MS.gene62409.t1 | MTR_0337s0050 | 67.500 | 40 | 13 | 0 | 195 | 234 | 14 | 53 | 1.27e-14 | 67.8 |
| MS.gene62409.t1 | MTR_0402s0040 | 26.000 | 150 | 96 | 3 | 20 | 169 | 71 | 205 | 1.52e-13 | 70.5 |
| MS.gene62409.t1 | MTR_4g035820 | 24.031 | 258 | 173 | 8 | 16 | 253 | 89 | 343 | 1.82e-12 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62409.t1 | AT4G38180 | 29.717 | 212 | 136 | 3 | 20 | 231 | 118 | 316 | 1.74e-25 | 105 |
| MS.gene62409.t1 | AT2G27110 | 26.901 | 171 | 108 | 4 | 62 | 230 | 107 | 262 | 1.92e-12 | 67.4 |
| MS.gene62409.t1 | AT2G27110 | 26.901 | 171 | 108 | 4 | 62 | 230 | 107 | 262 | 1.92e-12 | 67.4 |
| MS.gene62409.t1 | AT5G28530 | 23.276 | 232 | 151 | 6 | 5 | 223 | 84 | 301 | 5.17e-12 | 66.2 |
| MS.gene62409.t1 | AT5G28530 | 23.276 | 232 | 151 | 6 | 5 | 223 | 84 | 301 | 5.17e-12 | 66.2 |
| MS.gene62409.t1 | AT5G28530 | 23.276 | 232 | 151 | 6 | 5 | 223 | 84 | 301 | 5.53e-12 | 65.9 |
| MS.gene62409.t1 | AT1G10240 | 25.758 | 198 | 131 | 7 | 43 | 230 | 102 | 293 | 8.06e-12 | 65.5 |
| MS.gene62409.t1 | AT1G10240 | 25.758 | 198 | 131 | 7 | 43 | 230 | 102 | 293 | 8.06e-12 | 65.5 |
| MS.gene62409.t1 | AT2G27110 | 35.366 | 82 | 53 | 0 | 149 | 230 | 36 | 117 | 1.42e-11 | 64.7 |
| MS.gene62409.t1 | AT4G15090 | 27.011 | 174 | 124 | 3 | 59 | 231 | 121 | 292 | 8.43e-11 | 62.4 |
| MS.gene62409.t1 | AT4G15090 | 27.011 | 174 | 124 | 3 | 59 | 231 | 121 | 292 | 8.43e-11 | 62.4 |
| MS.gene62409.t1 | AT4G15090 | 27.011 | 174 | 124 | 3 | 59 | 231 | 121 | 292 | 8.43e-11 | 62.4 |
| MS.gene62409.t1 | AT4G15090 | 27.011 | 174 | 124 | 3 | 59 | 231 | 121 | 292 | 8.43e-11 | 62.4 |
| MS.gene62409.t1 | AT4G15090 | 27.011 | 174 | 124 | 3 | 59 | 231 | 121 | 292 | 8.43e-11 | 62.4 |
Find 36 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACAAGATTATGCTCTATT+TGG | 0.198611 | 8.2:+14122240 | MS.gene62409:CDS |
| CCTTATTCTGAAAATTTGTA+TGG | 0.314405 | 8.2:+14122390 | MS.gene62409:CDS |
| GCCGATATTTATTGTTTGTT+CGG | 0.361178 | 8.2:-14122290 | None:intergenic |
| TATCGGCCGTTTACAGCAAC+TGG | 0.387085 | 8.2:+14122306 | MS.gene62409:CDS |
| TTCTCATACTCAACAAGAAC+TGG | 0.395633 | 8.2:-14121760 | None:intergenic |
| TTGTTTCCAGTTGCTGTAAA+CGG | 0.405482 | 8.2:-14122312 | None:intergenic |
| ACCGAACAAACAATAAATAT+CGG | 0.406375 | 8.2:+14122289 | MS.gene62409:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 8.2:+14121937 | MS.gene62409:CDS |
| AGCTGGAATATATTTGAAGT+TGG | 0.433136 | 8.2:+14121819 | MS.gene62409:CDS |
| TGAATGTCTATAAGATATAT+AGG | 0.445219 | 8.2:+14121851 | MS.gene62409:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 8.2:+14121802 | MS.gene62409:CDS |
| CGTTATGCTTGTAACAAAGA+AGG | 0.449439 | 8.2:+14121685 | MS.gene62409:CDS |
| GCTTTAATAAATTCTAAAGC+TGG | 0.450264 | 8.2:+14121967 | MS.gene62409:CDS |
| CTCAAGTAGCGCAAATGCTC+TGG | 0.460440 | 8.2:-14121907 | None:intergenic |
| ACAAAATAAGAAATCGGATA+AGG | 0.469178 | 8.2:+14121651 | MS.gene62409:CDS |
| GAAGTGATGGGTCAAGGAGT+TGG | 0.484292 | 8.2:+14122012 | MS.gene62409:CDS |
| TTACAACCAACCCTTTCCTC+TGG | 0.485330 | 8.2:-14121796 | None:intergenic |
| TGTCTTCTGCAGCATCAACT+TGG | 0.487243 | 8.2:-14122185 | None:intergenic |
| CAATTCACAAAATAAGAAAT+CGG | 0.490263 | 8.2:+14121645 | MS.gene62409:CDS |
| GAGAACATCTTATGAAGTGA+TGG | 0.499875 | 8.2:+14121999 | MS.gene62409:CDS |
| TTTAAGTCCGAAAATCGAAA+AGG | 0.508080 | 8.2:-14122051 | None:intergenic |
| TGTCTATAAGATATATAGGT+GGG | 0.523740 | 8.2:+14121855 | MS.gene62409:CDS |
| TAACAAAGAAGGTTTCAAGA+AGG | 0.538422 | 8.2:+14121696 | MS.gene62409:CDS |
| CGTCAAAAGAAGATGGTGGC+TGG | 0.540133 | 8.2:+14122093 | MS.gene62409:CDS |
| AACAAAGAAGGTTTCAAGAA+GGG | 0.545537 | 8.2:+14121697 | MS.gene62409:CDS |
| CTTATTCTGAAAATTTGTAT+GGG | 0.551924 | 8.2:+14122391 | MS.gene62409:CDS |
| GACAACTCGTCAAAAGAAGA+TGG | 0.552238 | 8.2:+14122086 | MS.gene62409:CDS |
| AAATGCTCTGGTTTATGTAA+TGG | 0.552634 | 8.2:-14121895 | None:intergenic |
| GTGATGGGTCAAGGAGTTGG+AGG | 0.568802 | 8.2:+14122015 | MS.gene62409:CDS |
| TCTTATGAAGTGATGGGTCA+AGG | 0.589029 | 8.2:+14122006 | MS.gene62409:CDS |
| AGAACATCTTATGAAGTGAT+GGG | 0.601988 | 8.2:+14122000 | MS.gene62409:CDS |
| AATGAAGCTCTCTCTAAACC+AGG | 0.618452 | 8.2:+14122147 | MS.gene62409:CDS |
| AGAACTGGACTGTCACATGT+TGG | 0.620791 | 8.2:-14121745 | None:intergenic |
| GGGTCAAGGAGTTGGAGGCA+CGG | 0.621882 | 8.2:+14122020 | MS.gene62409:CDS |
| ATGTCTATAAGATATATAGG+TGG | 0.643489 | 8.2:+14121854 | MS.gene62409:CDS |
| AACTCGTCAAAAGAAGATGG+TGG | 0.679247 | 8.2:+14122089 | MS.gene62409:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAATTCACAAAATAAGAAAT+CGG | + | chr8.2:14121645-14121664 | MS.gene62409:CDS | 20.0% |
| !! | TGAATGTCTATAAGATATAT+AGG | + | chr8.2:14121851-14121870 | MS.gene62409:CDS | 20.0% |
| !!! | AAGACATAACTAGTATTTTT+TGG | + | chr8.2:14122202-14122221 | MS.gene62409:CDS | 20.0% |
| !!! | AGACATAACTAGTATTTTTT+GGG | + | chr8.2:14122203-14122222 | MS.gene62409:CDS | 20.0% |
| !!! | CTTATTCTGAAAATTTGTAT+GGG | + | chr8.2:14122391-14122410 | MS.gene62409:CDS | 20.0% |
| ! | ACAAAATAAGAAATCGGATA+AGG | + | chr8.2:14121651-14121670 | MS.gene62409:CDS | 25.0% |
| ! | ACCGAACAAACAATAAATAT+CGG | + | chr8.2:14122289-14122308 | MS.gene62409:CDS | 25.0% |
| ! | ATGTCTATAAGATATATAGG+TGG | + | chr8.2:14121854-14121873 | MS.gene62409:CDS | 25.0% |
| ! | TGTCTATAAGATATATAGGT+GGG | + | chr8.2:14121855-14121874 | MS.gene62409:CDS | 25.0% |
| !! | CCATACAAATTTTCAGAATA+AGG | - | chr8.2:14122393-14122412 | None:intergenic | 25.0% |
| !! | CCTTATTCTGAAAATTTGTA+TGG | + | chr8.2:14122390-14122409 | MS.gene62409:CDS | 25.0% |
| !! | GAATTTTCCTTTTCGATTTT+CGG | + | chr8.2:14122044-14122063 | MS.gene62409:CDS | 25.0% |
| !!! | CTTATTTTGTGAATTGTTCT+TGG | - | chr8.2:14121641-14121660 | None:intergenic | 25.0% |
| !!! | GCTTTAATAAATTCTAAAGC+TGG | + | chr8.2:14121967-14121986 | MS.gene62409:CDS | 25.0% |
| AACAAAGAAGGTTTCAAGAA+GGG | + | chr8.2:14121697-14121716 | MS.gene62409:CDS | 30.0% | |
| AGAACATCTTATGAAGTGAT+GGG | + | chr8.2:14122000-14122019 | MS.gene62409:CDS | 30.0% | |
| CAACAAGATTATGCTCTATT+TGG | + | chr8.2:14122240-14122259 | MS.gene62409:CDS | 30.0% | |
| GCCGATATTTATTGTTTGTT+CGG | - | chr8.2:14122293-14122312 | None:intergenic | 30.0% | |
| TAAAGAAAAACCAGAGGAAA+GGG | + | chr8.2:14121786-14121805 | MS.gene62409:CDS | 30.0% | |
| TAACAAAGAAGGTTTCAAGA+AGG | + | chr8.2:14121696-14121715 | MS.gene62409:CDS | 30.0% | |
| TGGATATCATAGTAAAAACC+TGG | - | chr8.2:14122168-14122187 | None:intergenic | 30.0% | |
| TTTAAGTCCGAAAATCGAAA+AGG | - | chr8.2:14122054-14122073 | None:intergenic | 30.0% | |
| ! | AAATGCTCTGGTTTATGTAA+TGG | - | chr8.2:14121898-14121917 | None:intergenic | 30.0% |
| ! | AGCTGGAATATATTTGAAGT+TGG | + | chr8.2:14121819-14121838 | MS.gene62409:CDS | 30.0% |
| !!! | ACGTCTGACATTTTTTGTTT+TGG | + | chr8.2:14122348-14122367 | MS.gene62409:CDS | 30.0% |
| CGAGAAGTAAATGAAGTTCA+AGG | + | chr8.2:14121937-14121956 | MS.gene62409:CDS | 35.0% | |
| CGTTATGCTTGTAACAAAGA+AGG | + | chr8.2:14121685-14121704 | MS.gene62409:CDS | 35.0% | |
| CTAAAGAAAAACCAGAGGAA+AGG | + | chr8.2:14121785-14121804 | MS.gene62409:CDS | 35.0% | |
| GAAAGCTAAAGAAAAACCAG+AGG | + | chr8.2:14121780-14121799 | MS.gene62409:CDS | 35.0% | |
| GAGAACATCTTATGAAGTGA+TGG | + | chr8.2:14121999-14122018 | MS.gene62409:CDS | 35.0% | |
| GTCTGCTTAATCCTAAAAAC+AGG | + | chr8.2:14121722-14121741 | MS.gene62409:CDS | 35.0% | |
| TTCTCATACTCAACAAGAAC+TGG | - | chr8.2:14121763-14121782 | None:intergenic | 35.0% | |
| TTGTTTCCAGTTGCTGTAAA+CGG | - | chr8.2:14122315-14122334 | None:intergenic | 35.0% | |
| !!! | ACTAGTATTTTTTGGGCAGA+TGG | + | chr8.2:14122210-14122229 | MS.gene62409:CDS | 35.0% |
| AACTCGTCAAAAGAAGATGG+TGG | + | chr8.2:14122089-14122108 | MS.gene62409:CDS | 40.0% | |
| AATGAAGCTCTCTCTAAACC+AGG | + | chr8.2:14122147-14122166 | MS.gene62409:CDS | 40.0% | |
| GACAACTCGTCAAAAGAAGA+TGG | + | chr8.2:14122086-14122105 | MS.gene62409:CDS | 40.0% | |
| !! | TCACATGTTGGCCTGTTTTT+AGG | - | chr8.2:14121736-14121755 | None:intergenic | 40.0% |
| !! | TCTTATGAAGTGATGGGTCA+AGG | + | chr8.2:14122006-14122025 | MS.gene62409:CDS | 40.0% |
| AGAACTGGACTGTCACATGT+TGG | - | chr8.2:14121748-14121767 | None:intergenic | 45.0% | |
| GAAAAACCAGAGGAAAGGGT+TGG | + | chr8.2:14121790-14121809 | MS.gene62409:CDS | 45.0% | |
| TGTCTTCTGCAGCATCAACT+TGG | - | chr8.2:14122188-14122207 | None:intergenic | 45.0% | |
| TTACAACCAACCCTTTCCTC+TGG | - | chr8.2:14121799-14121818 | None:intergenic | 45.0% | |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | + | chr8.2:14121802-14121821 | MS.gene62409:CDS | 45.0% |
| CTCAAGTAGCGCAAATGCTC+TGG | - | chr8.2:14121910-14121929 | None:intergenic | 50.0% | |
| TATCGGCCGTTTACAGCAAC+TGG | + | chr8.2:14122306-14122325 | MS.gene62409:CDS | 50.0% | |
| ! | CGTCAAAAGAAGATGGTGGC+TGG | + | chr8.2:14122093-14122112 | MS.gene62409:CDS | 50.0% |
| !! | GAAGTGATGGGTCAAGGAGT+TGG | + | chr8.2:14122012-14122031 | MS.gene62409:CDS | 50.0% |
| !! | GTGATGGGTCAAGGAGTTGG+AGG | + | chr8.2:14122015-14122034 | MS.gene62409:CDS | 55.0% |
| GGGTCAAGGAGTTGGAGGCA+CGG | + | chr8.2:14122020-14122039 | MS.gene62409:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 14121628 | 14122434 | 14121628 | ID=MS.gene62409 |
| chr8.2 | mRNA | 14121628 | 14122434 | 14121628 | ID=MS.gene62409.t1;Parent=MS.gene62409 |
| chr8.2 | exon | 14121628 | 14122434 | 14121628 | ID=MS.gene62409.t1.exon1;Parent=MS.gene62409.t1 |
| chr8.2 | CDS | 14121628 | 14122434 | 14121628 | ID=cds.MS.gene62409.t1;Parent=MS.gene62409.t1 |
| Gene Sequence |
| Protein sequence |
