AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62443

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62443.t1 MTR_4g113970 94.737 247 12 1 1 246 1 247 1.38e-177 491
MS.gene62443.t1 MTR_4g113860 61.791 335 91 8 1 326 1 307 5.78e-140 399
MS.gene62443.t1 MTR_4g113940 55.488 328 86 10 1 326 1 270 1.19e-110 322
MS.gene62443.t1 MTR_3g050390 51.237 283 77 7 44 326 7 228 8.60e-87 261
MS.gene62443.t1 MTR_4g113890 47.130 331 68 9 1 325 1 230 1.04e-74 229
MS.gene62443.t1 MTR_8g066730 33.775 302 141 10 71 326 86 374 3.89e-40 145
MS.gene62443.t1 MTR_4g113980 87.805 82 10 0 245 326 9 90 1.74e-32 116
MS.gene62443.t1 MTR_8g066730 35.922 206 91 6 71 244 86 282 2.54e-30 116
MS.gene62443.t1 MTR_3g466460 52.688 93 37 2 240 326 3 94 1.80e-19 83.6
MS.gene62443.t1 MTR_3g028610 42.857 91 47 1 154 244 96 181 7.44e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62443.t1 AT3G19360 44.753 324 131 10 42 326 59 373 2.05e-76 239
MS.gene62443.t1 AT3G19360 44.753 324 131 10 42 326 59 373 2.05e-76 239
MS.gene62443.t1 AT1G32360 32.550 298 159 8 71 326 85 382 3.84e-36 134
MS.gene62443.t1 AT2G35430 30.144 209 102 9 138 326 66 250 6.52e-16 76.6
MS.gene62443.t1 AT2G35430 35.849 106 46 4 77 168 71 168 2.86e-11 63.2

Find 102 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGTTCTCCAAAGTCTTT+TGG 0.221074 8.2:-14400286 MS.gene62443:CDS
GCAATAAGCATTGGAAATAT+TGG 0.228785 8.2:-14400304 MS.gene62443:CDS
ATGAGGATCCTGCTAGGTTT+AGG 0.244285 8.2:-14400357 MS.gene62443:CDS
TGGGGATGGTTCTAACAATT+TGG 0.253072 8.2:-14400266 MS.gene62443:CDS
CTAACAATTTGGAAGGCATT+AGG 0.254194 8.2:-14400255 MS.gene62443:CDS
AAGAAGATCAATGGTATTTA+TGG 0.269407 8.2:-14398046 MS.gene62443:CDS
CACTTACTTAGGTTGAAATT+AGG 0.275359 8.2:-14398070 MS.gene62443:CDS
CAAGGCAGCCACCACCAAAT+TGG 0.283685 8.2:-14400474 MS.gene62443:CDS
TGAGGATCCTGCTAGGTTTA+GGG 0.310738 8.2:-14400356 MS.gene62443:CDS
CTAGGTTTAGGGATGATTCT+TGG 0.317545 8.2:-14400345 MS.gene62443:CDS
GCAACACCTGTTGAGGCTTT+TGG 0.324209 8.2:+14398163 None:intergenic
TTCCAATAAGCGCTCCTTGC+TGG 0.328577 8.2:+14400220 None:intergenic
AGCCAGCAAGGAGCGCTTAT+TGG 0.331978 8.2:-14400222 MS.gene62443:CDS
TCAATGGTATTTATGGTGAT+TGG 0.333200 8.2:-14398039 MS.gene62443:CDS
CACTTCAATGATATCGTTGT+TGG 0.339985 8.2:+14400801 None:intergenic
ATACAGGGAGCTGTCCCTTT+GGG 0.341839 8.2:-14400174 MS.gene62443:CDS
ACCTGAACTTAGTGCAGATT+CGG 0.365276 8.2:+14400620 None:intergenic
TCAAAGTGATCGTTATTGAT+TGG 0.371371 8.2:+14400766 None:intergenic
TAACAATTTGGAAGGCATTA+GGG 0.381170 8.2:-14400254 MS.gene62443:CDS
CAAAGTGATCGTTATTGATT+GGG 0.381558 8.2:+14400767 None:intergenic
TAAGGCAGCCACCGCGGAAT+TGG 0.383871 8.2:-14400543 MS.gene62443:CDS
ATGCTGCTTGTTCCTCGGTT+TGG 0.390724 8.2:+14400655 None:intergenic
AACGGTGAGGAATGTCCTTA+TGG 0.402090 8.2:-14400400 MS.gene62443:CDS
AGGTTTGGTACTTGTAGAAA+TGG 0.405516 8.2:-14400601 MS.gene62443:CDS
CCCGAATCTGCACTAAGTTC+AGG 0.412306 8.2:-14400621 MS.gene62443:CDS
ATCTGCACTAAGTTCAGGTT+TGG 0.426557 8.2:-14400616 MS.gene62443:CDS
CCTGAACTTAGTGCAGATTC+GGG 0.429469 8.2:+14400621 None:intergenic
CATACAGGGAGCTGTCCCTT+TGG 0.435906 8.2:-14400175 MS.gene62443:CDS
GATTGATATGCCCTCCTTCA+AGG 0.436742 8.2:-14400693 MS.gene62443:CDS
CTGCTTGTTCCTCGGTTTGG+TGG 0.444819 8.2:+14400658 None:intergenic
GCAGCCACCGCGGAATTGGC+AGG 0.444919 8.2:-14400539 MS.gene62443:CDS
AAGGCAGCCACCACCAAATT+GGG 0.448398 8.2:-14400473 MS.gene62443:CDS
CTGTGTCAATGGCAGCATAC+AGG 0.450029 8.2:-14400190 MS.gene62443:CDS
GAACCATCCCCAAAAGACTT+TGG 0.450065 8.2:+14400277 None:intergenic
CTTGTTCCTCGGTTTGGTGG+AGG 0.456539 8.2:+14400661 None:intergenic
CACTTTGCTCATGGAGAAGC+AGG 0.457533 8.2:-14400142 MS.gene62443:intron
ATAAGAAGAAGCACTTACTT+AGG 0.466516 8.2:-14398081 MS.gene62443:CDS
AGCAATTCTCGCCCTCTTGA+AGG 0.471550 8.2:+14400735 None:intergenic
AATATCGTCTGGAAAATTCA+TGG 0.472650 8.2:+14400861 None:intergenic
CTACAGGTTGGAATATCGTC+TGG 0.476700 8.2:+14400850 None:intergenic
AAAATTGCAATTACGCTCAC+GGG 0.487194 8.2:-14400576 MS.gene62443:CDS
ACTTCACCAAAAGCCTCAAC+AGG 0.492958 8.2:-14398169 MS.gene62443:CDS
TCGTTATTGATTGGGATTTG+AGG 0.495130 8.2:+14400775 None:intergenic
GGAGGCTGAATCATCCTTGA+AGG 0.501461 8.2:+14400679 None:intergenic
GAGACCTGTCACTTTGCTCA+TGG 0.502581 8.2:-14400151 MS.gene62443:CDS
CTCACGGGGCTGATGAGATA+AGG 0.504892 8.2:-14400561 MS.gene62443:CDS
TTGATCCTCGCAACGACGAA+CGG 0.512205 8.2:-14400510 MS.gene62443:CDS
CATGACAAAGGAAGAGGAGC+AGG 0.513127 8.2:-14398104 MS.gene62443:CDS
TGATCTTCATCCCAATTTGG+TGG 0.514081 8.2:+14400463 None:intergenic
GAGTTACAAGCTCCTGGTGG+AGG 0.514447 8.2:-14398247 MS.gene62443:CDS
AAGCTACACTTATCACCATA+AGG 0.515098 8.2:+14400385 None:intergenic
GAAGGTAGGAGTAGAATCAT+TGG 0.517961 8.2:+14398141 None:intergenic
TTCTCCATGAGCAAAGTGAC+AGG 0.523166 8.2:+14400147 None:intergenic
TTCTTCATGAGGATCCTGCT+AGG 0.523992 8.2:-14400363 MS.gene62443:CDS
GGAAGACAAAGCTGTGTCAA+TGG 0.532349 8.2:-14400201 MS.gene62443:CDS
ATCATTGGCAACACCTGTTG+AGG 0.534873 8.2:+14398156 None:intergenic
GAATCATCCCTAAACCTAGC+AGG 0.537317 8.2:+14400349 None:intergenic
TCTTCATCCCAATTTGGTGG+TGG 0.537541 8.2:+14400466 None:intergenic
GATGGTTCTAACAATTTGGA+AGG 0.539743 8.2:-14400262 MS.gene62443:CDS
GAGTGTTCTGCAATAAGCAT+TGG 0.542316 8.2:-14400313 MS.gene62443:CDS
CATGAGTAGCCTTGTGGTGA+AGG 0.545217 8.2:+14398123 None:intergenic
AAGTGACAGGTCTCCCCAAA+GGG 0.549845 8.2:+14400160 None:intergenic
TGTGTCAATGGCAGCATACA+GGG 0.552109 8.2:-14400189 MS.gene62443:CDS
CTGCGGTGGTGACATCCTAC+AGG 0.555913 8.2:+14400834 None:intergenic
GAAAATTGCAATTACGCTCA+CGG 0.559384 8.2:-14400577 MS.gene62443:CDS
AAATCAGAGTTACAAGCTCC+TGG 0.561857 8.2:-14398253 MS.gene62443:intron
AAATTAGGCAAGAAGATCAA+TGG 0.564267 8.2:-14398055 MS.gene62443:CDS
CAACGACGAACGGCTTCAGA+TGG 0.574258 8.2:-14400500 MS.gene62443:CDS
CTTTGTCATGAGTAGCCTTG+TGG 0.576482 8.2:+14398117 None:intergenic
TCAGCTTCAATGCCTCCACC+AGG 0.581762 8.2:+14398235 None:intergenic
AGTAGCCTTGTGGTGAAGGT+AGG 0.585355 8.2:+14398127 None:intergenic
GGCTGAATCATCCTTGAAGG+AGG 0.587591 8.2:+14400682 None:intergenic
GCTACTCATGACAAAGGAAG+AGG 0.589258 8.2:-14398110 MS.gene62443:CDS
CTCGCCCTCTTGAAGGAATG+TGG 0.590307 8.2:+14400742 None:intergenic
TTGAGCCACATTCCTTCAAG+AGG 0.596203 8.2:-14400747 MS.gene62443:CDS
TACAGGGAGCTGTCCCTTTG+GGG 0.596640 8.2:-14400173 MS.gene62443:CDS
GGAGGGCATATCAATCCACT+AGG 0.602999 8.2:+14400700 None:intergenic
CTACTCCTACCTTCACCACA+AGG 0.611445 8.2:-14398132 MS.gene62443:CDS
GGGCTGTGAACAAGCCAGCA+AGG 0.614710 8.2:-14400234 MS.gene62443:CDS
TGAGCCACATTCCTTCAAGA+GGG 0.615845 8.2:-14400746 MS.gene62443:CDS
TAAGTGTAGCTTTCTTCATG+AGG 0.621800 8.2:-14400374 MS.gene62443:CDS
TGAAGCCGTTCGTCGTTGCG+AGG 0.623112 8.2:+14400505 None:intergenic
AAAGTGACAGGTCTCCCCAA+AGG 0.626382 8.2:+14400159 None:intergenic
CAGACGATATTCCAACCTGT+AGG 0.630277 8.2:-14400849 MS.gene62443:CDS
AGCTCCTGCCAATTCCGCGG+TGG 0.631980 8.2:+14400535 None:intergenic
CACAAGGCTACTCATGACAA+AGG 0.641025 8.2:-14398116 MS.gene62443:CDS
GAGGGCATATCAATCCACTA+GGG 0.647490 8.2:+14400701 None:intergenic
AACGGCTTCAGATGGAGACA+AGG 0.650858 8.2:-14400492 MS.gene62443:CDS
GCTGAATCATCCTTGAAGGA+GGG 0.652254 8.2:+14400683 None:intergenic
ACTAGCTCCTGCCAATTCCG+CGG 0.663260 8.2:+14400532 None:intergenic
GGTGGTGACATCCTACAGGT+TGG 0.681473 8.2:+14400838 None:intergenic
ATGAGATAAGGCAGCCACCG+CGG 0.683865 8.2:-14400549 MS.gene62443:CDS
TCAGAGTTACAAGCTCCTGG+TGG 0.696701 8.2:-14398250 MS.gene62443:intron
GGGCATATCAATCCACTAGG+GGG 0.701239 8.2:+14400703 None:intergenic
ACAACGATATCATTGAAGTG+AGG 0.706867 8.2:-14400798 MS.gene62443:CDS
ATTCAGCCTCCACCAAACCG+AGG 0.710090 8.2:-14400667 MS.gene62443:CDS
CAAGAAGTATTACAACGGTG+AGG 0.717786 8.2:-14400413 MS.gene62443:CDS
AAATTGCAATTACGCTCACG+GGG 0.722741 8.2:-14400575 MS.gene62443:CDS
AGGGCATATCAATCCACTAG+GGG 0.727577 8.2:+14400702 None:intergenic
CTGTGCAAGAAGTATTACAA+CGG 0.729247 8.2:-14400418 MS.gene62443:CDS
TTGGATGAAGCAAACTGCGG+TGG 0.740311 8.2:+14400820 None:intergenic
TTGTTGGATGAAGCAAACTG+CGG 0.811729 8.2:+14400817 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTTTTTTTGCATATT+TGG - chr8.2:14400512-14400531 MS.gene62443:CDS 10.0%
!! GTGATTGAATATTTAAATTT+AGG + chr8.2:14399557-14399576 None:intergenic 15.0%
!!! TTCTAATTCAAAAGTAATTT+TGG + chr8.2:14399785-14399804 None:intergenic 15.0%
!! AAATAAAAATTCCTGACAAT+GGG + chr8.2:14399514-14399533 None:intergenic 20.0%
!! AATCATCCATTATAATATGA+AGG + chr8.2:14399579-14399598 None:intergenic 20.0%
!! CAAAACCATTAATCAAAATA+GGG + chr8.2:14398805-14398824 None:intergenic 20.0%
!! TCAAAACCATTAATCAAAAT+AGG + chr8.2:14398806-14398825 None:intergenic 20.0%
!!! TTATATGTCATGCTTATTTT+TGG - chr8.2:14399984-14400003 MS.gene62443:intron 20.0%
! AAAAATCTGGTATAGAAACA+TGG + chr8.2:14399216-14399235 None:intergenic 25.0%
! AAGAAGATCAATGGTATTTA+TGG - chr8.2:14400840-14400859 MS.gene62443:CDS 25.0%
! AATAAAAATTCCTGACAATG+GGG + chr8.2:14399513-14399532 None:intergenic 25.0%
! AATGTTTGACCGTAAAATTA+AGG + chr8.2:14400373-14400392 None:intergenic 25.0%
! ACAATTACAATAGGTAAGAA+AGG - chr8.2:14400539-14400558 MS.gene62443:CDS 25.0%
! ATTAATCAAAATAGGGAGAA+TGG + chr8.2:14398798-14398817 None:intergenic 25.0%
! CAAATAAAAATTCCTGACAA+TGG + chr8.2:14399515-14399534 None:intergenic 25.0%
! CAATCACCTTCATATTATAA+TGG - chr8.2:14399570-14399589 MS.gene62443:intron 25.0%
! TTATAACAATTGAAGCTGTT+TGG - chr8.2:14400565-14400584 MS.gene62443:CDS 25.0%
!! TTTGGAACAACAATTACAAT+AGG - chr8.2:14400530-14400549 MS.gene62443:CDS 25.0%
!!! AAGTAATTTTGGTACATACT+TGG + chr8.2:14399774-14399793 None:intergenic 25.0%
!!! ATGTTTCTATACCAGATTTT+TGG - chr8.2:14399215-14399234 MS.gene62443:intron 25.0%
!!! ATTCTCCCTATTTTGATTAA+TGG - chr8.2:14398797-14398816 MS.gene62443:intron 25.0%
!!! TTCTTTCAGTTTTGAAGTAA+TGG - chr8.2:14399063-14399082 MS.gene62443:intron 25.0%
AAAGATAAAAGCAGTCACTT+CGG + chr8.2:14400145-14400164 None:intergenic 30.0%
AAATTAGGCAAGAAGATCAA+TGG - chr8.2:14400831-14400850 MS.gene62443:CDS 30.0%
AATATAAAACCTCGCAAATG+AGG + chr8.2:14400334-14400353 None:intergenic 30.0%
AATCAACAAATGTAAAGCTG+TGG + chr8.2:14399636-14399655 None:intergenic 30.0%
ATATCATACACAACAACGAT+AGG + chr8.2:14399044-14399063 None:intergenic 30.0%
ATGATACCAAGTACGAATAT+CGG + chr8.2:14400081-14400100 None:intergenic 30.0%
TCAAAGTGATCGTTATTGAT+TGG + chr8.2:14398123-14398142 None:intergenic 30.0%
TCAATGGTATTTATGGTGAT+TGG - chr8.2:14400847-14400866 MS.gene62443:CDS 30.0%
TCTTAGTGTTGTCAAATTCA+TGG - chr8.2:14399118-14399137 MS.gene62443:intron 30.0%
TGTCCGACTCATAAAAAAAT+TGG - chr8.2:14400299-14400318 MS.gene62443:CDS 30.0%
TGTTGTGTATGATATTTCCA+TGG - chr8.2:14398951-14398970 MS.gene62443:intron 30.0%
TTCTATGATGATTGTGTAGA+AGG - chr8.2:14398870-14398889 MS.gene62443:intron 30.0%
TTGTTAAATAGCGACTATAG+CGG + chr8.2:14399447-14399466 None:intergenic 30.0%
! AATGGATGATTGCTATTGTA+TGG - chr8.2:14399588-14399607 MS.gene62443:intron 30.0%
! CAAAGTGATCGTTATTGATT+GGG + chr8.2:14398122-14398141 None:intergenic 30.0%
! CTTTACATTTGTTGATTGAC+TGG - chr8.2:14399639-14399658 MS.gene62443:intron 30.0%
! TATCCTAAAAAGCATATCAC+TGG - chr8.2:14399383-14399402 MS.gene62443:intron 30.0%
! TCAAACATTTTTAATGCAGC+TGG - chr8.2:14400384-14400403 MS.gene62443:CDS 30.0%
! TGTCGCAATCCTTAATTTTA+CGG - chr8.2:14400361-14400380 MS.gene62443:CDS 30.0%
! TTTTGATCTTCATCCCAATT+TGG + chr8.2:14398429-14398448 None:intergenic 30.0%
!! ATAAGAAGAAGCACTTACTT+AGG - chr8.2:14400805-14400824 MS.gene62443:CDS 30.0%
!! CACTTACTTAGGTTGAAATT+AGG - chr8.2:14400816-14400835 MS.gene62443:CDS 30.0%
!! GATACACTAGGATAGTTTTA+CGG - chr8.2:14399824-14399843 MS.gene62443:intron 30.0%
!! GCAATAAGCATTGGAAATAT+TGG - chr8.2:14398582-14398601 MS.gene62443:intron 30.0%
!! TAACAATTTGGAAGGCATTA+GGG - chr8.2:14398632-14398651 MS.gene62443:intron 30.0%
!!! CCTCCAATTTTTTTATGAGT+CGG + chr8.2:14400305-14400324 None:intergenic 30.0%
AAAATTGCAATTACGCTCAC+GGG - chr8.2:14398310-14398329 MS.gene62443:intron 35.0%
AAGCTACACTTATCACCATA+AGG + chr8.2:14398504-14398523 None:intergenic 35.0%
ACAACGATATCATTGAAGTG+AGG - chr8.2:14398088-14398107 MS.gene62443:CDS 35.0%
AGCTATTACACTGTTGCATA+GGG + chr8.2:14399346-14399365 None:intergenic 35.0%
AGTTTATGTCAAACCAACCA+TGG + chr8.2:14398971-14398990 None:intergenic 35.0%
ATACACAGTGTTGTCAATTG+TGG - chr8.2:14399290-14399309 MS.gene62443:intron 35.0%
ATCAGCCAGAACAAAATACA+TGG + chr8.2:14400594-14400613 None:intergenic 35.0%
ATCATACACAACAACGAAAG+TGG + chr8.2:14398944-14398963 None:intergenic 35.0%
CACTTCAATGATATCGTTGT+TGG + chr8.2:14398088-14398107 None:intergenic 35.0%
CCGACTCATAAAAAAATTGG+AGG - chr8.2:14400302-14400321 MS.gene62443:CDS 35.0%
GAAAATTGCAATTACGCTCA+CGG - chr8.2:14398309-14398328 MS.gene62443:intron 35.0%
GAGTCGGACATTAAATTTAC+AGG + chr8.2:14400289-14400308 None:intergenic 35.0%
GTGTATGATATTTCCATGGT+TGG - chr8.2:14398955-14398974 MS.gene62443:intron 35.0%
TAACCTTATCCATGCAACAT+AGG - chr8.2:14400102-14400121 MS.gene62443:intron 35.0%
TAAGTGTAGCTTTCTTCATG+AGG - chr8.2:14398512-14398531 MS.gene62443:intron 35.0%
TATGGTAGCTGGAACATAAT+CGG + chr8.2:14399144-14399163 None:intergenic 35.0%
TGTCAAAAACCTTAACGTCT+TGG - chr8.2:14400431-14400450 MS.gene62443:CDS 35.0%
TGTTAAATAGCGACTATAGC+GGG + chr8.2:14399446-14399465 None:intergenic 35.0%
TTGTATCCGATATTCGTACT+TGG - chr8.2:14400072-14400091 MS.gene62443:intron 35.0%
! AAAAAATGCTGCTTGTTCCT+CGG + chr8.2:14398239-14398258 None:intergenic 35.0%
! AGCTTTTGTTGAGCTTACTA+TGG + chr8.2:14400688-14400707 None:intergenic 35.0%
! AGGTTTGGTACTTGTAGAAA+TGG - chr8.2:14398285-14398304 MS.gene62443:intron 35.0%
! GACTGGAACTTTTAGATGTT+TGG - chr8.2:14399656-14399675 MS.gene62443:intron 35.0%
! GGAAAACTTGTTTGTACGAT+CGG - chr8.2:14399084-14399103 MS.gene62443:intron 35.0%
! GGTTTCCATGTATTTTGTTC+TGG - chr8.2:14400586-14400605 MS.gene62443:CDS 35.0%
! TCGTTATTGATTGGGATTTG+AGG + chr8.2:14398114-14398133 None:intergenic 35.0%
! TTATGATAATGCTGCCCTAT+TGG - chr8.2:14399263-14399282 MS.gene62443:intron 35.0%
!! ATTGGTTCTCCAAAGTCTTT+TGG - chr8.2:14398600-14398619 MS.gene62443:intron 35.0%
!! CTAACAATTTGGAAGGCATT+AGG - chr8.2:14398631-14398650 MS.gene62443:intron 35.0%
!! CTGTGCAAGAAGTATTACAA+CGG - chr8.2:14398468-14398487 MS.gene62443:intron 35.0%
!! GATGGTTCTAACAATTTGGA+AGG - chr8.2:14398624-14398643 MS.gene62443:intron 35.0%
!! TGTCCAGTGATATGCTTTTT+AGG + chr8.2:14399389-14399408 None:intergenic 35.0%
!! TTGGTTCTCCAAAGTCTTTT+GGG - chr8.2:14398601-14398620 MS.gene62443:intron 35.0%
AAATCAGAGTTACAAGCTCC+TGG - chr8.2:14400633-14400652 MS.gene62443:CDS 40.0%
AAATTGCAATTACGCTCACG+GGG - chr8.2:14398311-14398330 MS.gene62443:intron 40.0%
AAGGTATGCGACAAAAACTG+TGG + chr8.2:14400457-14400476 None:intergenic 40.0%
ACACCTATGTTGCATGGATA+AGG + chr8.2:14400108-14400127 None:intergenic 40.0%
ACACTGTGTATACCTCCAAT+AGG + chr8.2:14399281-14399300 None:intergenic 40.0%
ACCAAAAGACGAAACAGGAT+TGG + chr8.2:14400251-14400270 None:intergenic 40.0%
ACCTGAACTTAGTGCAGATT+CGG + chr8.2:14398269-14398288 None:intergenic 40.0%
ACTATCCTAGTGTATCCAAG+TGG + chr8.2:14399820-14399839 None:intergenic 40.0%
ATAATAGCCATTGCAGCCTA+TGG + chr8.2:14399162-14399181 None:intergenic 40.0%
ATACTCCACTTGGATACACT+AGG - chr8.2:14399812-14399831 MS.gene62443:intron 40.0%
CACTGTGTATACCTCCAATA+GGG + chr8.2:14399280-14399299 None:intergenic 40.0%
CAGAAACCAAAAGACGAAAC+AGG + chr8.2:14400256-14400275 None:intergenic 40.0%
CAGCTATTACACTGTTGCAT+AGG + chr8.2:14399347-14399366 None:intergenic 40.0%
CATACACAACAACGATAGGA+AGG + chr8.2:14399040-14399059 None:intergenic 40.0%
CTAGGTTTAGGGATGATTCT+TGG - chr8.2:14398541-14398560 MS.gene62443:intron 40.0%
CTGCATAGTCGATCAAAAGA+AGG + chr8.2:14399701-14399720 None:intergenic 40.0%
GAAGGTAGGAGTAGAATCAT+TGG + chr8.2:14400748-14400767 None:intergenic 40.0%
GAGTGTTCTGCAATAAGCAT+TGG - chr8.2:14398573-14398592 MS.gene62443:intron 40.0%
GATTATGTTCCAGCTACCAT+AGG - chr8.2:14399143-14399162 MS.gene62443:intron 40.0%
GCAGCATTATCATAAGTGGA+AGG + chr8.2:14399258-14399277 None:intergenic 40.0%
GCATTATCATAAGTGGAAGG+TGG + chr8.2:14399255-14399274 None:intergenic 40.0%
GCTGCAATGGCTATTATATC+TGG - chr8.2:14399165-14399184 MS.gene62443:intron 40.0%
GTTAAATAGCGACTATAGCG+GGG + chr8.2:14399445-14399464 None:intergenic 40.0%
TAGGGCAGCATTATCATAAG+TGG + chr8.2:14399262-14399281 None:intergenic 40.0%
TATCCATGCAACATAGGTGT+CGG - chr8.2:14400108-14400127 MS.gene62443:intron 40.0%
TCACACTCTTACTGTACTTG+TGG - chr8.2:14399741-14399760 MS.gene62443:intron 40.0%
TGATCTTCATCCCAATTTGG+TGG + chr8.2:14398426-14398445 None:intergenic 40.0%
TTGTTGGATGAAGCAAACTG+CGG + chr8.2:14398072-14398091 None:intergenic 40.0%
! ATCTGCACTAAGTTCAGGTT+TGG - chr8.2:14398270-14398289 MS.gene62443:intron 40.0%
! TCCAATCCTGTTTCGTCTTT+TGG - chr8.2:14400247-14400266 MS.gene62443:CDS 40.0%
! TGGTTCTCCAAAGTCTTTTG+GGG - chr8.2:14398602-14398621 MS.gene62443:intron 40.0%
! TGTTCTGGCTGATGAAGTAT+TGG - chr8.2:14400601-14400620 MS.gene62443:CDS 40.0%
! TTTTGATCGACTATGCAGCA+TGG - chr8.2:14399703-14399722 MS.gene62443:intron 40.0%
!! CAAGAAGTATTACAACGGTG+AGG - chr8.2:14398473-14398492 MS.gene62443:intron 40.0%
!! TGGGGATGGTTCTAACAATT+TGG - chr8.2:14398620-14398639 MS.gene62443:intron 40.0%
!!! CGTCTTTTGGTTTCTGCTAA+TGG - chr8.2:14400260-14400279 MS.gene62443:CDS 40.0%
AAACATGGCATTGCAGCCTA+TGG + chr8.2:14399201-14399220 None:intergenic 45.0%
AAACTGTGGCCAAGACGTTA+AGG + chr8.2:14400443-14400462 None:intergenic 45.0%
AACGGTGAGGAATGTCCTTA+TGG - chr8.2:14398486-14398505 MS.gene62443:intron 45.0%
ACAGCAGCTAGCCAAAAATC+TGG + chr8.2:14399229-14399248 None:intergenic 45.0%
ACTTCACCAAAAGCCTCAAC+AGG - chr8.2:14400717-14400736 MS.gene62443:CDS 45.0%
AGGGCATATCAATCCACTAG+GGG + chr8.2:14398187-14398206 None:intergenic 45.0%
AGGTACATTCCTCATTTGCG+AGG - chr8.2:14400322-14400341 MS.gene62443:CDS 45.0%
ATCATTGGCAACACCTGTTG+AGG + chr8.2:14400733-14400752 None:intergenic 45.0%
ATGAGGATCCTGCTAGGTTT+AGG - chr8.2:14398529-14398548 MS.gene62443:intron 45.0%
ATTCCTGACAATGGGGTTGA+TGG + chr8.2:14399506-14399525 None:intergenic 45.0%
CACAAGGCTACTCATGACAA+AGG - chr8.2:14400770-14400789 MS.gene62443:CDS 45.0%
CAGACGATATTCCAACCTGT+AGG - chr8.2:14398037-14398056 MS.gene62443:CDS 45.0%
CCTGAACTTAGTGCAGATTC+GGG + chr8.2:14398268-14398287 None:intergenic 45.0%
GAAACGCTAGATACTCCACT+TGG - chr8.2:14399802-14399821 MS.gene62443:intron 45.0%
GAACCATCCCCAAAAGACTT+TGG + chr8.2:14398612-14398631 None:intergenic 45.0%
GAATCATCCCTAAACCTAGC+AGG + chr8.2:14398540-14398559 None:intergenic 45.0%
GATTGATATGCCCTCCTTCA+AGG - chr8.2:14398193-14398212 MS.gene62443:CDS 45.0%
GCTACTCATGACAAAGGAAG+AGG - chr8.2:14400776-14400795 MS.gene62443:CDS 45.0%
GCTGAATCATCCTTGAAGGA+GGG + chr8.2:14398206-14398225 None:intergenic 45.0%
GGAAGACAAAGCTGTGTCAA+TGG - chr8.2:14398685-14398704 MS.gene62443:intron 45.0%
TAAAAGCAGTCACTTCGGTG+TGG + chr8.2:14400140-14400159 None:intergenic 45.0%
TGAGCCACATTCCTTCAAGA+GGG - chr8.2:14398140-14398159 MS.gene62443:CDS 45.0%
TGAGGATCCTGCTAGGTTTA+GGG - chr8.2:14398530-14398549 MS.gene62443:intron 45.0%
TGTGTCAATGGCAGCATACA+GGG - chr8.2:14398697-14398716 MS.gene62443:intron 45.0%
TTACCGACACCTATGTTGCA+TGG + chr8.2:14400114-14400133 None:intergenic 45.0%
TTCTCCATGAGCAAAGTGAC+AGG + chr8.2:14398742-14398761 None:intergenic 45.0%
TTCTGCGAAGACAAGGCAAA+GGG + chr8.2:14399013-14399032 None:intergenic 45.0%
TTCTTCATGAGGATCCTGCT+AGG - chr8.2:14398523-14398542 MS.gene62443:intron 45.0%
TTGAGCCACATTCCTTCAAG+AGG - chr8.2:14398139-14398158 MS.gene62443:CDS 45.0%
TTTCTGCGAAGACAAGGCAA+AGG + chr8.2:14399014-14399033 None:intergenic 45.0%
! AAAAGCAGTCACTTCGGTGT+GGG + chr8.2:14400139-14400158 None:intergenic 45.0%
! CTACAGGTTGGAATATCGTC+TGG + chr8.2:14398039-14398058 None:intergenic 45.0%
! CTTTGTCATGAGTAGCCTTG+TGG + chr8.2:14400772-14400791 None:intergenic 45.0%
! GAGGGCATATCAATCCACTA+GGG + chr8.2:14398188-14398207 None:intergenic 45.0%
! GTTGAGGCTTTTGGTGAAGT+TGG + chr8.2:14400717-14400736 None:intergenic 45.0%
! TCTTCATCCCAATTTGGTGG+TGG + chr8.2:14398423-14398442 None:intergenic 45.0%
! TGATAATGCTGCCCTATTGG+AGG - chr8.2:14399266-14399285 MS.gene62443:intron 45.0%
! TTCCTGACAATGGGGTTGAT+GGG + chr8.2:14399505-14399524 None:intergenic 45.0%
!! ATCTGGCTTGTTTCCACCAT+AGG - chr8.2:14399182-14399201 MS.gene62443:intron 45.0%
!! TCTCCAAAGTCTTTTGGGGA+TGG - chr8.2:14398606-14398625 MS.gene62443:intron 45.0%
!!! GATTTTATTTTTTTAAATAA+AGG + chr8.2:14400476-14400495 None:intergenic 5.0%
AAAGTGACAGGTCTCCCCAA+AGG + chr8.2:14398730-14398749 None:intergenic 50.0%
AACGGCTTCAGATGGAGACA+AGG - chr8.2:14398394-14398413 MS.gene62443:intron 50.0%
AAGGCAAAGGGGCAACAAAC+AGG + chr8.2:14399001-14399020 None:intergenic 50.0%
AAGGCAGCCACCACCAAATT+GGG - chr8.2:14398413-14398432 MS.gene62443:intron 50.0%
AAGTGACAGGTCTCCCCAAA+GGG + chr8.2:14398729-14398748 None:intergenic 50.0%
AGCAATTCTCGCCCTCTTGA+AGG + chr8.2:14398154-14398173 None:intergenic 50.0%
AGGCACTTTCTGCGAAGACA+AGG + chr8.2:14399020-14399039 None:intergenic 50.0%
ATACAGGGAGCTGTCCCTTT+GGG - chr8.2:14398712-14398731 MS.gene62443:intron 50.0%
CAAAGGGGCAACAAACAGGA+GGG + chr8.2:14398997-14399016 None:intergenic 50.0%
CACTTTGCTCATGGAGAAGC+AGG - chr8.2:14398744-14398763 MS.gene62443:intron 50.0%
CATGACAAAGGAAGAGGAGC+AGG - chr8.2:14400782-14400801 MS.gene62443:CDS 50.0%
CCCGAATCTGCACTAAGTTC+AGG - chr8.2:14398265-14398284 MS.gene62443:intron 50.0%
CTACTCCTACCTTCACCACA+AGG - chr8.2:14400754-14400773 MS.gene62443:CDS 50.0%
CTGTGTCAATGGCAGCATAC+AGG - chr8.2:14398696-14398715 MS.gene62443:intron 50.0%
GCTGATAACAATCCCCCTAG+TGG - chr8.2:14398171-14398190 MS.gene62443:CDS 50.0%
GGAGGCTGAATCATCCTTGA+AGG + chr8.2:14398210-14398229 None:intergenic 50.0%
GGCTGAATCATCCTTGAAGG+AGG + chr8.2:14398207-14398226 None:intergenic 50.0%
GGGCATATCAATCCACTAGG+GGG + chr8.2:14398186-14398205 None:intergenic 50.0%
TCAGAGTTACAAGCTCCTGG+TGG - chr8.2:14400636-14400655 MS.gene62443:CDS 50.0%
TCTGCGAAGACAAGGCAAAG+GGG + chr8.2:14399012-14399031 None:intergenic 50.0%
TTCCAATAAGCGCTCCTTGC+TGG + chr8.2:14398669-14398688 None:intergenic 50.0%
TTCCCATCAACCCCATTGTC+AGG - chr8.2:14399500-14399519 MS.gene62443:intron 50.0%
TTGATCCTCGCAACGACGAA+CGG - chr8.2:14398376-14398395 MS.gene62443:intron 50.0%
TTGGATGAAGCAAACTGCGG+TGG + chr8.2:14398069-14398088 None:intergenic 50.0%
! ATAGCGGGGCTACTGCATAT+AGG + chr8.2:14399431-14399450 None:intergenic 50.0%
! ATGCTGCTTGTTCCTCGGTT+TGG + chr8.2:14398234-14398253 None:intergenic 50.0%
! GCAACACCTGTTGAGGCTTT+TGG + chr8.2:14400726-14400745 None:intergenic 50.0%
! GGAGGGCATATCAATCCACT+AGG + chr8.2:14398189-14398208 None:intergenic 50.0%
!! AGTAGCCTTGTGGTGAAGGT+AGG + chr8.2:14400762-14400781 None:intergenic 50.0%
!! CATGAGTAGCCTTGTGGTGA+AGG + chr8.2:14400766-14400785 None:intergenic 50.0%
!! GAGACCTGTCACTTTGCTCA+TGG - chr8.2:14398735-14398754 MS.gene62443:intron 50.0%
ACTAGCTCCTGCCAATTCCG+CGG + chr8.2:14398357-14398376 None:intergenic 55.0%
ATGAGATAAGGCAGCCACCG+CGG - chr8.2:14398337-14398356 MS.gene62443:intron 55.0%
ATTCAGCCTCCACCAAACCG+AGG - chr8.2:14398219-14398238 MS.gene62443:CDS 55.0%
CAACGACGAACGGCTTCAGA+TGG - chr8.2:14398386-14398405 MS.gene62443:intron 55.0%
CAAGGCAGCCACCACCAAAT+TGG - chr8.2:14398412-14398431 MS.gene62443:intron 55.0%
CATACAGGGAGCTGTCCCTT+TGG - chr8.2:14398711-14398730 MS.gene62443:intron 55.0%
CATGGCATTGCAGCCTATGG+TGG + chr8.2:14399198-14399217 None:intergenic 55.0%
CCAGCTACCATAGGCTGCAA+TGG - chr8.2:14399152-14399171 MS.gene62443:intron 55.0%
CCATTGCAGCCTATGGTAGC+TGG + chr8.2:14399155-14399174 None:intergenic 55.0%
CTCACGGGGCTGATGAGATA+AGG - chr8.2:14398325-14398344 MS.gene62443:intron 55.0%
CTCGCCCTCTTGAAGGAATG+TGG + chr8.2:14398147-14398166 None:intergenic 55.0%
GAGTTACAAGCTCCTGGTGG+AGG - chr8.2:14400639-14400658 MS.gene62443:CDS 55.0%
GCAAAGGGGCAACAAACAGG+AGG + chr8.2:14398998-14399017 None:intergenic 55.0%
GGTGGTGACATCCTACAGGT+TGG + chr8.2:14398051-14398070 None:intergenic 55.0%
TACAGGGAGCTGTCCCTTTG+GGG - chr8.2:14398713-14398732 MS.gene62443:intron 55.0%
TCAGCTTCAATGCCTCCACC+AGG + chr8.2:14400654-14400673 None:intergenic 55.0%
!! AGCCAGCAAGGAGCGCTTAT+TGG - chr8.2:14398664-14398683 MS.gene62443:intron 55.0%
!! CTGCTTGTTCCTCGGTTTGG+TGG + chr8.2:14398231-14398250 None:intergenic 55.0%
!! CTTGTTCCTCGGTTTGGTGG+AGG + chr8.2:14398228-14398247 None:intergenic 55.0%
CTGCGGTGGTGACATCCTAC+AGG + chr8.2:14398055-14398074 None:intergenic 60.0%
GGGCTGTGAACAAGCCAGCA+AGG - chr8.2:14398652-14398671 MS.gene62443:intron 60.0%
TAAGGCAGCCACCGCGGAAT+TGG - chr8.2:14398343-14398362 MS.gene62443:intron 60.0%
TGAAGCCGTTCGTCGTTGCG+AGG + chr8.2:14398384-14398403 None:intergenic 60.0%
AGCTCCTGCCAATTCCGCGG+TGG + chr8.2:14398354-14398373 None:intergenic 65.0%
GCAGCCACCGCGGAATTGGC+AGG - chr8.2:14398347-14398366 MS.gene62443:intron 70.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 14398027 14400881 14398027 ID=MS.gene62443
chr8.2 mRNA 14398027 14400881 14398027 ID=MS.gene62443.t1;Parent=MS.gene62443
chr8.2 exon 14400143 14400881 14400143 ID=MS.gene62443.t1.exon1;Parent=MS.gene62443.t1
chr8.2 CDS 14400143 14400881 14400143 ID=cds.MS.gene62443.t1;Parent=MS.gene62443.t1
chr8.2 exon 14398027 14398268 14398027 ID=MS.gene62443.t1.exon2;Parent=MS.gene62443.t1
chr8.2 CDS 14398027 14398268 14398027 ID=cds.MS.gene62443.t1;Parent=MS.gene62443.t1
Gene Sequence

>MS.gene62443

ATGAATTTTCCAGACGATATTCCAACCTGTAGGATGTCACCACCGCAGTTTGCTTCATCCAACAACGATATCATTGAAGTGAGGCCTCAAATCCCAATCAATAACGATCACTTTGAGCCACATTCCTTCAAGAGGGCGAGAATTGCTGATAACAATCCCCCTAGTGGATTGATATGCCCTCCTTCAAGGATGATTCAGCCTCCACCAAACCGAGGAACAAGCAGCATTTTTTTCAAGACCCGAATCTGCACTAAGTTCAGGTTTGGTACTTGTAGAAATGGCGAAAATTGCAATTACGCTCACGGGGCTGATGAGATAAGGCAGCCACCGCGGAATTGGCAGGAGCTAGTTGATCCTCGCAACGACGAACGGCTTCAGATGGAGACAAGGCAGCCACCACCAAATTGGGATGAAGATCAAAAAATCATTCACAAGATGAAGCTGTGCAAGAAGTATTACAACGGTGAGGAATGTCCTTATGGTGATAAGTGTAGCTTTCTTCATGAGGATCCTGCTAGGTTTAGGGATGATTCTTGGAAAACAAGAGAGTGTTCTGCAATAAGCATTGGAAATATTGGTTCTCCAAAGTCTTTTGGGGATGGTTCTAACAATTTGGAAGGCATTAGGGCTGTGAACAAGCCAGCAAGGAGCGCTTATTGGAAGACAAAGCTGTGTCAATGGCAGCATACAGGGAGCTGTCCCTTTGGGGAGACCTGTCACTTTGCTCATGGAGAAGCAGGTAATGTTAACTCATCTGTTTGTCAGTTTCCATTCTCCCTATTTTGATTAATGGTTTTGATTTTAACTTTATCATATAATTCTACTGTTTTGCTTTTTGTGTTGTTCTATGATGATTGTGTAGAAGGTTATCTTCTCTGATATATGATGTACAAATTAATGTGTTTGAATGTGCGCCACTTTCGTTGTTGTGTATGATATTTCCATGGTTGGTTTGACATAAACTTGTCCCTCCTGTTTGTTGCCCCTTTGCCTTGTCTTCGCAGAAAGTGCCTTCCTATCGTTGTTGTGTATGATATTCTTTCAGTTTTGAAGTAATGGAAAACTTGTTTGTACGATCGGTGATTACTTTATCTTAGTGTTGTCAAATTCATGGCCGATTATGTTCCAGCTACCATAGGCTGCAATGGCTATTATATCTGGCTTGTTTCCACCATAGGCTGCAATGCCATGTTTCTATACCAGATTTTTGGCTAGCTGCTGTAATCCACCTTCCACTTATGATAATGCTGCCCTATTGGAGGTATACACAGTGTTGTCAATTGTGGATCACAAAAAATTGTGCTTTGTTCAAATTTCCCTATGCAACAGTGTAATAGCTGCTATTTCATATTATGTTATCCTAAAAAGCATATCACTGGACAAAAGCGATTTTTTCAAATTCCTATATGCAGTAGCCCCGCTATAGTCGCTATTTAACAACACTTGCATAATACTTGAATGAAATTTTGCTTTTTCCCATCAACCCCATTGTCAGGAATTTTTATTTGATAGAGATGACTAGATTTTCCTAAATTTAAATATTCAATCACCTTCATATTATAATGGATGATTGCTATTGTATGGTGACAAAAGTTAAAATTATTTTCCACAGCTTTACATTTGTTGATTGACTGGAACTTTTAGATGTTTGGAATAATTTGAGAAAGATTTCCTTCTTTTGATCGACTATGCAGCATGGTACTACAAATTGAAATCACACTCTTACTGTACTTGTGGTAACGTACCAAGTATGTACCAAAATTACTTTTGAATTAGAAACGCTAGATACTCCACTTGGATACACTAGGATAGTTTTACGGTATGTACAATAAATAAATAAAAATATGTTTATCGTTTAGTAAATACAAAAAAAAATATTTTCTTATATTTATTTTCTTATTCAGTTTTGATATGTGATCTAGTGAACGAAGAAAGACGTTTCTTTGTATATTTTATGTTATATGTCATGCTTATTTTTGGTTTCTATTTCTATACAAGCTTGTCATGTTTCTATCTTTCATAAATCTTTGTCATGTTCTATCTTCTTGTATCCGATATTCGTACTTGGTATCATATAACCTTATCCATGCAACATAGGTGTCGGTAATTCCCACACCGAAGTGACTGCTTTTATCTTTCAATCATATCATACAATTGAAATTTGAGTAATTGCTTTTGCTTGCGATTCTGAATAATCAACTATTATATTATGTTCATCTTTCCAATCCTGTTTCGTCTTTTGGTTTCTGCTAATGGTTGCCTGTAAATTTAATGTCCGACTCATAAAAAAATTGGAGGTACATTCCTCATTTGCGAGGTTTTATATTGCAGCAATGTCGCAATCCTTAATTTTACGGTCAAACATTTTTAATGCAGCTGGAGTTGCGACTTGTGATGACAAAGATGTCAAAAACCTTAACGTCTTGGCCACAGTTTTTGTCGCATACCTTTATTTAAAAAAATAAAATCTTGCTTATTTGTGTGATAATTTTTTTTTTGCATATTTGGAACAACAATTACAATAGGTAAGAAAGGTGATTATAACAATTGAAGCTGTTTGGTTTCCATGTATTTTGTTCTGGCTGATGAAGTATTGGTTGTCTTGCAAATCAGAGTTACAAGCTCCTGGTGGAGGCATTGAAGCTGAAGCTGCAGTTGCCATAGTAAGCTCAACAAAAGCTATCATTCCAACTTCACCAAAAGCCTCAACAGGTGTTGCCAATGATTCTACTCCTACCTTCACCACAAGGCTACTCATGACAAAGGAAGAGGAGCAGGATAAGAAGAAGCACTTACTTAGGTTGAAATTAGGCAAGAAGATCAATGGTATTTATGGTGATTGGATTGATGATTGA
Protein sequence

>MS.gene62443.t1

MNFPDDIPTCRMSPPQFASSNNDIIEVRPQIPINNDHFEPHSFKRARIADNNPPSGLICPPSRMIQPPPNRGTSSIFFKTRICTKFRFGTCRNGENCNYAHGADEIRQPPRNWQELVDPRNDERLQMETRQPPPNWDEDQKIIHKMKLCKKYYNGEECPYGDKCSFLHEDPARFRDDSWKTRECSAISIGNIGSPKSFGDGSNNLEGIRAVNKPARSAYWKTKLCQWQHTGSCPFGETCHFAHGEAELQAPGGGIEAEAAVAIVSSTKAIIPTSPKASTGVANDSTPTFTTRLLMTKEEEQDKKKHLLRLKLGKKINGIYGDWIDD