Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62446.t1 | XP_003609277.1 | 46.7 | 259 | 65 | 4 | 1 | 186 | 12 | 270 | 1.70E-48 | 202.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62446.t1 | Q9LT81 | 43.3 | 90 | 32 | 1 | 36 | 106 | 116 | 205 | 4.0e-14 | 79.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62446.t1 | G7JVB8 | 46.7 | 259 | 65 | 4 | 1 | 186 | 12 | 270 | 1.2e-48 | 202.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene62446.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62446.t1 | MTR_4g113940 | 56.371 | 259 | 40 | 4 | 1 | 186 | 12 | 270 | 6.00e-89 | 262 |
| MS.gene62446.t1 | MTR_4g113890 | 58.515 | 229 | 41 | 4 | 1 | 185 | 12 | 230 | 2.49e-80 | 239 |
| MS.gene62446.t1 | MTR_4g113860 | 34.551 | 301 | 87 | 5 | 1 | 191 | 12 | 312 | 6.53e-47 | 157 |
| MS.gene62446.t1 | MTR_4g113970 | 38.017 | 242 | 57 | 6 | 1 | 150 | 12 | 252 | 2.42e-36 | 127 |
| MS.gene62446.t1 | MTR_3g050390 | 36.652 | 221 | 89 | 4 | 20 | 191 | 15 | 233 | 3.73e-34 | 121 |
| MS.gene62446.t1 | MTR_3g466460 | 55.932 | 59 | 23 | 1 | 138 | 193 | 46 | 104 | 1.62e-14 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62446.t1 | AT3G19360 | 30.153 | 262 | 74 | 7 | 36 | 189 | 116 | 376 | 4.20e-23 | 95.5 |
| MS.gene62446.t1 | AT3G19360 | 30.153 | 262 | 74 | 7 | 36 | 189 | 116 | 376 | 4.20e-23 | 95.5 |
Find 49 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACCTAGCTTCTGCAAATT+TGG | 0.182814 | 8.2:+14418696 | None:intergenic |
| CCAAGTGTACGCTTCTTCTT+AGG | 0.267354 | 8.2:+14417975 | None:intergenic |
| AGCCAGATCAATCGTATTTA+CGG | 0.289560 | 8.2:-14417942 | MS.gene62446:CDS |
| CTCTGGAAACACACCTCATT+TGG | 0.330065 | 8.2:-14417888 | MS.gene62446:CDS |
| AATTTACAGTTATCTCTATA+TGG | 0.333739 | 8.2:+14418570 | None:intergenic |
| TCAATCGTATTTACGGTGAT+TGG | 0.347052 | 8.2:-14417935 | MS.gene62446:CDS |
| CATACCGGATATTGTTCCTT+TGG | 0.350886 | 8.2:-14418400 | MS.gene62446:CDS |
| TGAACAATAATGACTTCAAA+AGG | 0.385432 | 8.2:-14418926 | MS.gene62446:CDS |
| AGACTAAGCTGTGTCGCAAT+TGG | 0.427484 | 8.2:-14418426 | MS.gene62446:CDS |
| AGCAAGCAAGGGGCACTTAT+CGG | 0.435970 | 8.2:-14418450 | MS.gene62446:CDS |
| AGATTTGGTGCTTGTAGAAA+CGG | 0.437088 | 8.2:-14418768 | MS.gene62446:CDS |
| ACGGTGATTGGATTGATGAT+TGG | 0.443878 | 8.2:-14417923 | MS.gene62446:CDS |
| CAACGAAGATCAACTGCAGC+TGG | 0.445811 | 8.2:-14418667 | MS.gene62446:CDS |
| TCCTTGTTTATAGGCAGAGC+AGG | 0.451853 | 8.2:-14418038 | MS.gene62446:intron |
| CCTAAGAAGAAGCGTACACT+TGG | 0.453400 | 8.2:-14417975 | MS.gene62446:CDS |
| CATTCCAAAGGAACAATATC+CGG | 0.460353 | 8.2:+14418396 | None:intergenic |
| CTAATAATTTGGAAGGCAGC+AGG | 0.471854 | 8.2:-14418483 | MS.gene62446:intron |
| AGCTTCTGCAAATTTGGTGG+TGG | 0.473172 | 8.2:+14418702 | None:intergenic |
| CACCGTAAATACGATTGATC+TGG | 0.481235 | 8.2:+14417940 | None:intergenic |
| GGGCTGTGAACAAGCAAGCA+AGG | 0.482646 | 8.2:-14418462 | MS.gene62446:CDS |
| ATGTACCCGACTTGTGGAAA+AGG | 0.484051 | 8.2:-14418009 | MS.gene62446:CDS |
| TGTGCAAGAAGTATTGCAAT+GGG | 0.488339 | 8.2:-14418602 | MS.gene62446:CDS |
| CTCTTTGCTCATGATGAAGA+AGG | 0.508905 | 8.2:-14418367 | MS.gene62446:intron |
| CCTAGCTTCTGCAAATTTGG+TGG | 0.512657 | 8.2:+14418699 | None:intergenic |
| GGCTGTGAACAAGCAAGCAA+GGG | 0.513346 | 8.2:-14418461 | MS.gene62446:CDS |
| TTCTGAATGTACCCGACTTG+TGG | 0.526232 | 8.2:-14418015 | MS.gene62446:CDS |
| TGCAGTTGATCTTCGTTGCA+CGG | 0.530704 | 8.2:+14418672 | None:intergenic |
| ATCATGGAGCTGAAGAGATA+AGG | 0.532117 | 8.2:-14418728 | MS.gene62446:CDS |
| AGGAGGGCATATCAATGCAT+TGG | 0.539478 | 8.2:+14418887 | None:intergenic |
| GAACAATAATGACTTCAAAA+GGG | 0.552156 | 8.2:-14418925 | MS.gene62446:CDS |
| TGTCGCAATTGGAAGCATAC+CGG | 0.563682 | 8.2:-14418415 | MS.gene62446:CDS |
| CTGTGCAAGAAGTATTGCAA+TGG | 0.572239 | 8.2:-14418603 | MS.gene62446:CDS |
| TGTTTCCAGAGCATAACAAA+AGG | 0.581785 | 8.2:+14417900 | None:intergenic |
| AATAATGACTTCAAAAGGGA+AGG | 0.582018 | 8.2:-14418921 | MS.gene62446:CDS |
| GAAGAATGCTTATCCAAATG+AGG | 0.582982 | 8.2:+14417875 | None:intergenic |
| TAATAATTTGGAAGGCAGCA+GGG | 0.584634 | 8.2:-14418482 | MS.gene62446:intron |
| CACATCAATGCATCAGAAGG+AGG | 0.586467 | 8.2:+14418870 | None:intergenic |
| TTGTTCAATGAAGTGAATTG+CGG | 0.591801 | 8.2:+14418942 | None:intergenic |
| AACGAAGATCAACTGCAGCT+GGG | 0.593604 | 8.2:-14418666 | MS.gene62446:CDS |
| CCACCAAATTTGCAGAAGCT+AGG | 0.604649 | 8.2:-14418699 | MS.gene62446:CDS |
| AAAGAGACAGTTCATTCCAA+AGG | 0.613697 | 8.2:+14418384 | None:intergenic |
| GGTCACATCAATGCATCAGA+AGG | 0.614755 | 8.2:+14418867 | None:intergenic |
| ACATCAATGCATCAGAAGGA+GGG | 0.627126 | 8.2:+14418871 | None:intergenic |
| GCTGTGAACAAGCAAGCAAG+GGG | 0.637136 | 8.2:-14418460 | MS.gene62446:CDS |
| CCAAATTTGCAGAAGCTAGG+TGG | 0.641093 | 8.2:-14418696 | MS.gene62446:CDS |
| CAAGAAGTATTGCAATGGGG+AGG | 0.655957 | 8.2:-14418598 | MS.gene62446:CDS |
| CTAAGAAGAAGCGTACACTT+GGG | 0.659038 | 8.2:-14417974 | MS.gene62446:CDS |
| TTCAATGAAGTGAATTGCGG+CGG | 0.670436 | 8.2:+14418945 | None:intergenic |
| GTGCAAGAAGTATTGCAATG+GGG | 0.763140 | 8.2:-14418601 | MS.gene62446:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGATGAAAAAATCATACAA+AGG | - | chr8.2:14418203-14418222 | MS.gene62446:intron | 20.0% |
| !! | AATTTACAGTTATCTCTATA+TGG | + | chr8.2:14418268-14418287 | None:intergenic | 20.0% |
| !!! | CTCATTTTGATTATTGATTT+TGG | - | chr8.2:14418500-14418519 | MS.gene62446:intron | 20.0% |
| ! | AAAAATTGCAACTTTGATCA+TGG | - | chr8.2:14418091-14418110 | MS.gene62446:intron | 25.0% |
| ! | ACTTATCGGAACTAATAATT+TGG | - | chr8.2:14418341-14418360 | MS.gene62446:intron | 25.0% |
| ! | GAACAATAATGACTTCAAAA+GGG | - | chr8.2:14417910-14417929 | MS.gene62446:CDS | 25.0% |
| ! | TGAACAATAATGACTTCAAA+AGG | - | chr8.2:14417909-14417928 | MS.gene62446:CDS | 25.0% |
| !!! | TGTTTCATGTTTGATTTGAA+TGG | - | chr8.2:14418692-14418711 | MS.gene62446:CDS | 25.0% |
| AATAATGACTTCAAAAGGGA+AGG | - | chr8.2:14417914-14417933 | MS.gene62446:CDS | 30.0% | |
| ATCGGAACTAATAATTTGGA+AGG | - | chr8.2:14418345-14418364 | MS.gene62446:intron | 30.0% | |
| TAAGTACTCTCTTGATTTCA+AGG | + | chr8.2:14418326-14418345 | None:intergenic | 30.0% | |
| TTGTTCAATGAAGTGAATTG+CGG | + | chr8.2:14417896-14417915 | None:intergenic | 30.0% | |
| ! | ATGATTCCATCCTTGTTTAT+AGG | - | chr8.2:14418788-14418807 | MS.gene62446:intron | 30.0% |
| !! | GAAATCAAGAGAGTACTTAT+CGG | - | chr8.2:14418327-14418346 | MS.gene62446:intron | 30.0% |
| AAAGAGACAGTTCATTCCAA+AGG | + | chr8.2:14418454-14418473 | None:intergenic | 35.0% | |
| AGCCAGATCAATCGTATTTA+CGG | - | chr8.2:14418893-14418912 | MS.gene62446:CDS | 35.0% | |
| ATTTGTACCGAGTTCAGATT+TGG | - | chr8.2:14418052-14418071 | MS.gene62446:intron | 35.0% | |
| CATTCCAAAGGAACAATATC+CGG | + | chr8.2:14418442-14418461 | None:intergenic | 35.0% | |
| TAATAATTTGGAAGGCAGCA+GGG | - | chr8.2:14418353-14418372 | MS.gene62446:intron | 35.0% | |
| TCAATCGTATTTACGGTGAT+TGG | - | chr8.2:14418900-14418919 | MS.gene62446:CDS | 35.0% | |
| TGCAAAAATGTTCCTTCCTA+AGG | - | chr8.2:14418753-14418772 | MS.gene62446:CDS | 35.0% | |
| TGTTTCCAGAGCATAACAAA+AGG | + | chr8.2:14418938-14418957 | None:intergenic | 35.0% | |
| ! | AGGAACATTTTTGCAAGACA+AGG | + | chr8.2:14418748-14418767 | None:intergenic | 35.0% |
| ! | ATTTTTGCAAGACAAGGCAA+AGG | + | chr8.2:14418742-14418761 | None:intergenic | 35.0% |
| ! | TCATTTTCCATCAGAACCTT+AGG | + | chr8.2:14418772-14418791 | None:intergenic | 35.0% |
| ! | TTTTTGCAAGACAAGGCAAA+GGG | + | chr8.2:14418741-14418760 | None:intergenic | 35.0% |
| !! | AGATTTGGTGCTTGTAGAAA+CGG | - | chr8.2:14418067-14418086 | MS.gene62446:intron | 35.0% |
| !! | TGATTTGTTTGATTGAGGCA+AGG | + | chr8.2:14418008-14418027 | None:intergenic | 35.0% |
| !! | TGCTCTGATTTGTTTGATTG+AGG | + | chr8.2:14418013-14418032 | None:intergenic | 35.0% |
| !! | TGTGCAAGAAGTATTGCAAT+GGG | - | chr8.2:14418233-14418252 | MS.gene62446:intron | 35.0% |
| ACAAGCACCAAATCTGAACT+CGG | + | chr8.2:14418062-14418081 | None:intergenic | 40.0% | |
| ACATCAATGCATCAGAAGGA+GGG | + | chr8.2:14417967-14417986 | None:intergenic | 40.0% | |
| ATCATGGAGCTGAAGAGATA+AGG | - | chr8.2:14418107-14418126 | MS.gene62446:intron | 40.0% | |
| ATGAAGATCCTGCACAGTTT+AGG | - | chr8.2:14418293-14418312 | MS.gene62446:intron | 40.0% | |
| CACCGTAAATACGATTGATC+TGG | + | chr8.2:14418898-14418917 | None:intergenic | 40.0% | |
| CATACCGGATATTGTTCCTT+TGG | - | chr8.2:14418435-14418454 | MS.gene62446:CDS | 40.0% | |
| CTAATAATTTGGAAGGCAGC+AGG | - | chr8.2:14418352-14418371 | MS.gene62446:intron | 40.0% | |
| CTCTTTGCTCATGATGAAGA+AGG | - | chr8.2:14418468-14418487 | MS.gene62446:CDS | 40.0% | |
| TGAAGATCCTGCACAGTTTA+GGG | - | chr8.2:14418294-14418313 | MS.gene62446:intron | 40.0% | |
| TTCAATGAAGTGAATTGCGG+CGG | + | chr8.2:14417893-14417912 | None:intergenic | 40.0% | |
| TTTCCATCAGAACCTTAGGA+AGG | + | chr8.2:14418768-14418787 | None:intergenic | 40.0% | |
| ! | ACGGTGATTGGATTGATGAT+TGG | - | chr8.2:14418912-14418931 | MS.gene62446:CDS | 40.0% |
| ! | CTAAGAAGAAGCGTACACTT+GGG | - | chr8.2:14418861-14418880 | MS.gene62446:intron | 40.0% |
| ! | CTTCTTCCTTTTCCACAAGT+CGG | + | chr8.2:14418835-14418854 | None:intergenic | 40.0% |
| ! | TTCTTCCTTTTCCACAAGTC+GGG | + | chr8.2:14418834-14418853 | None:intergenic | 40.0% |
| !! | CTGTGCAAGAAGTATTGCAA+TGG | - | chr8.2:14418232-14418251 | MS.gene62446:intron | 40.0% |
| !! | GTGCAAGAAGTATTGCAATG+GGG | - | chr8.2:14418234-14418253 | MS.gene62446:intron | 40.0% |
| !!! | ATTGGCCTTTTGTTATGCTC+TGG | - | chr8.2:14418930-14418949 | MS.gene62446:CDS | 40.0% |
| AACGAAGATCAACTGCAGCT+GGG | - | chr8.2:14418169-14418188 | MS.gene62446:intron | 45.0% | |
| AAGGCAAAGGGAAAACCAGT+AGG | + | chr8.2:14418729-14418748 | None:intergenic | 45.0% | |
| ACCTGCTCTGCCTATAAACA+AGG | + | chr8.2:14418801-14418820 | None:intergenic | 45.0% | |
| ACTGTGCAGGATCTTCATGA+AGG | + | chr8.2:14418291-14418310 | None:intergenic | 45.0% | |
| AGACTAAGCTGTGTCGCAAT+TGG | - | chr8.2:14418409-14418428 | MS.gene62446:CDS | 45.0% | |
| ATGGAAAATGCTCCTCCTAC+TGG | - | chr8.2:14418711-14418730 | MS.gene62446:CDS | 45.0% | |
| CACATCAATGCATCAGAAGG+AGG | + | chr8.2:14417968-14417987 | None:intergenic | 45.0% | |
| CCAAATTTGCAGAAGCTAGG+TGG | - | chr8.2:14418139-14418158 | MS.gene62446:intron | 45.0% | |
| CCAAGTGTACGCTTCTTCTT+AGG | + | chr8.2:14418863-14418882 | None:intergenic | 45.0% | |
| CCACCAAATTTGCAGAAGCT+AGG | - | chr8.2:14418136-14418155 | MS.gene62446:intron | 45.0% | |
| CCACCTAGCTTCTGCAAATT+TGG | + | chr8.2:14418142-14418161 | None:intergenic | 45.0% | |
| CCTAGCTTCTGCAAATTTGG+TGG | + | chr8.2:14418139-14418158 | None:intergenic | 45.0% | |
| GAATCATCCCTAAACTGTGC+AGG | + | chr8.2:14418304-14418323 | None:intergenic | 45.0% | |
| GCTCTGCCTATAAACAAGGA+TGG | + | chr8.2:14418797-14418816 | None:intergenic | 45.0% | |
| GGTCACATCAATGCATCAGA+AGG | + | chr8.2:14417971-14417990 | None:intergenic | 45.0% | |
| TGCAGTTGATCTTCGTTGCA+CGG | + | chr8.2:14418166-14418185 | None:intergenic | 45.0% | |
| ! | AGCTTCTGCAAATTTGGTGG+TGG | + | chr8.2:14418136-14418155 | None:intergenic | 45.0% |
| ! | AGGAGGGCATATCAATGCAT+TGG | + | chr8.2:14417951-14417970 | None:intergenic | 45.0% |
| ! | ATGTACCCGACTTGTGGAAA+AGG | - | chr8.2:14418826-14418845 | MS.gene62446:intron | 45.0% |
| ! | CCTAAGAAGAAGCGTACACT+TGG | - | chr8.2:14418860-14418879 | MS.gene62446:intron | 45.0% |
| ! | CTCTGGAAACACACCTCATT+TGG | - | chr8.2:14418947-14418966 | MS.gene62446:CDS | 45.0% |
| ! | GTTCCTTCCTAAGGTTCTGA+TGG | - | chr8.2:14418762-14418781 | MS.gene62446:CDS | 45.0% |
| ! | TCCTTGTTTATAGGCAGAGC+AGG | - | chr8.2:14418797-14418816 | MS.gene62446:intron | 45.0% |
| ! | TGTCGCAATTGGAAGCATAC+CGG | - | chr8.2:14418420-14418439 | MS.gene62446:CDS | 45.0% |
| ! | TTCTGAATGTACCCGACTTG+TGG | - | chr8.2:14418820-14418839 | MS.gene62446:intron | 45.0% |
| !! | CAAGAAGTATTGCAATGGGG+AGG | - | chr8.2:14418237-14418256 | MS.gene62446:intron | 45.0% |
| CAACGAAGATCAACTGCAGC+TGG | - | chr8.2:14418168-14418187 | MS.gene62446:intron | 50.0% | |
| CATTGATGTGACCTCCTCCA+AGG | - | chr8.2:14417978-14417997 | MS.gene62446:CDS | 50.0% | |
| GCAAAGGGAAAACCAGTAGG+AGG | + | chr8.2:14418726-14418745 | None:intergenic | 50.0% | |
| GCTGTGAACAAGCAAGCAAG+GGG | - | chr8.2:14418375-14418394 | MS.gene62446:CDS | 50.0% | |
| GGCTGTGAACAAGCAAGCAA+GGG | - | chr8.2:14418374-14418393 | MS.gene62446:CDS | 50.0% | |
| !! | AGCAAGCAAGGGGCACTTAT+CGG | - | chr8.2:14418385-14418404 | MS.gene62446:CDS | 50.0% |
| GATGTGACCTCCTCCAAGGA+TGG | - | chr8.2:14417982-14418001 | MS.gene62446:CDS | 55.0% | |
| GATTGAGGCAAGGCCATCCT+TGG | + | chr8.2:14417998-14418017 | None:intergenic | 55.0% | |
| GGGCTGTGAACAAGCAAGCA+AGG | - | chr8.2:14418373-14418392 | MS.gene62446:CDS | 55.0% | |
| TGAGGCAAGGCCATCCTTGG+AGG | + | chr8.2:14417995-14418014 | None:intergenic | 60.0% | |
| GGCAAGGCCATCCTTGGAGG+AGG | + | chr8.2:14417992-14418011 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 14417884 | 14418973 | 14417884 | ID=MS.gene62446 |
| chr8.2 | mRNA | 14417884 | 14418973 | 14417884 | ID=MS.gene62446.t1;Parent=MS.gene62446 |
| chr8.2 | exon | 14418873 | 14418973 | 14418873 | ID=MS.gene62446.t1.exon1;Parent=MS.gene62446.t1 |
| chr8.2 | CDS | 14418873 | 14418973 | 14418873 | ID=cds.MS.gene62446.t1;Parent=MS.gene62446.t1 |
| chr8.2 | exon | 14418577 | 14418788 | 14418577 | ID=MS.gene62446.t1.exon2;Parent=MS.gene62446.t1 |
| chr8.2 | CDS | 14418577 | 14418788 | 14418577 | ID=cds.MS.gene62446.t1;Parent=MS.gene62446.t1 |
| chr8.2 | exon | 14418368 | 14418490 | 14418368 | ID=MS.gene62446.t1.exon3;Parent=MS.gene62446.t1 |
| chr8.2 | CDS | 14418368 | 14418490 | 14418368 | ID=cds.MS.gene62446.t1;Parent=MS.gene62446.t1 |
| chr8.2 | exon | 14417884 | 14418047 | 14417884 | ID=MS.gene62446.t1.exon4;Parent=MS.gene62446.t1 |
| chr8.2 | CDS | 14417884 | 14418047 | 14417884 | ID=cds.MS.gene62446.t1;Parent=MS.gene62446.t1 |
| Gene Sequence |
| Protein sequence |
