AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62450.t1 MTR_4g113940 72.963 270 33 2 1 230 1 270 1.63e-139 392
MS.gene62450.t1 MTR_4g113890 84.810 237 21 2 1 229 1 230 5.34e-139 389
MS.gene62450.t1 MTR_4g113860 45.130 308 90 7 1 230 1 307 2.74e-81 246
MS.gene62450.t1 MTR_4g113970 45.344 247 54 6 1 167 1 246 2.18e-58 185
MS.gene62450.t1 MTR_3g050390 41.250 240 75 8 31 230 15 228 1.03e-45 152
MS.gene62450.t1 MTR_3g466460 61.176 85 10 2 169 230 10 94 1.26e-23 92.4
MS.gene62450.t1 MTR_8g066730 36.364 110 44 1 54 137 91 200 1.42e-17 79.7
MS.gene62450.t1 MTR_8g066730 36.364 110 44 1 54 137 91 200 1.88e-17 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62450.t1 AT3G19360 34.228 298 93 8 31 230 81 373 1.77e-45 155
MS.gene62450.t1 AT3G19360 34.228 298 93 8 31 230 81 373 1.77e-45 155
MS.gene62450.t1 AT2G35430 33.166 199 91 9 55 230 71 250 1.85e-19 84.3
MS.gene62450.t1 AT1G32360 33.913 115 45 2 54 137 90 204 3.01e-15 74.3

Find 61 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAACTAGCTTCTGCGAATT+TGG 0.155359 8.2:+14453651 None:intergenic
ATGAAGATCCTGCACAATTT+AGG 0.163729 8.2:-14453527 MS.gene62450:CDS
CCAAGTATACGTTTCTTCTT+AGG 0.214369 8.2:+14452530 None:intergenic
TGAAGATCCTGCACAATTTA+GGG 0.278155 8.2:-14453526 MS.gene62450:CDS
AGTCAGATCAATCGTATTTA+CGG 0.310380 8.2:-14452497 MS.gene62450:CDS
GATACAGGATATTGTTCCTT+TGG 0.336419 8.2:-14453465 MS.gene62450:CDS
TCAATCGTATTTACGGTGAT+TGG 0.347052 8.2:-14452490 MS.gene62450:CDS
ATTTGTACTAAGTTCAGATT+TGG 0.359410 8.2:-14453738 MS.gene62450:CDS
CACAATTTAGGGGCACTTAT+CGG 0.370314 8.2:-14453515 MS.gene62450:CDS
AATTGCAGCTTCAGCTTCAA+TGG 0.390999 8.2:+14452622 None:intergenic
AAATCAGAGTTACAAGTTCC+TGG 0.400098 8.2:-14452650 MS.gene62450:intron
GACTTGCAGGGGAGGTGGTA+GGG 0.419543 8.2:+14452579 None:intergenic
TGCAAGTCAACTTGTGGAAA+AGG 0.437938 8.2:-14452564 MS.gene62450:CDS
CCTAAGAAGAAACGTATACT+TGG 0.441257 8.2:-14452530 MS.gene62450:CDS
AGATTTGGTGCTTGTAGAAA+CGG 0.444340 8.2:-14453723 MS.gene62450:CDS
CTGAGGCGGTGACATCTTAG+AGG 0.449778 8.2:+14453890 None:intergenic
TTAGAGGTTGGAATATCATC+TGG 0.452783 8.2:+14453906 None:intergenic
CACTTTGCTCATGGTGAAGA+AGG 0.463092 8.2:-14453432 MS.gene62450:intron
TGTCTCAAGTGGAAGGATAC+AGG 0.464303 8.2:-14453480 MS.gene62450:CDS
ACAAATTGCAACTTTGCTCA+TGG 0.469357 8.2:-14453699 MS.gene62450:CDS
CTGATCTGTTTGATTGAGGA+AGG 0.475163 8.2:+14453784 None:intergenic
CTCATGGGGCTGAAGAGATA+AGG 0.488249 8.2:-14453683 MS.gene62450:CDS
TGTGCAAGAAGTATAGCAAT+GGG 0.488339 8.2:-14453587 MS.gene62450:CDS
CAAATTGCAACTTTGCTCAT+GGG 0.490368 8.2:-14453698 MS.gene62450:CDS
TGACTTGCAGGGGAGGTGGT+AGG 0.490726 8.2:+14452578 None:intergenic
GATTGAGGAAGGGTCATCCT+TGG 0.496966 8.2:+14453795 None:intergenic
AGCTTCTGCGAATTTGGAGG+TGG 0.504943 8.2:+14453657 None:intergenic
CACAAGTTGACTTGCAGGGG+AGG 0.509364 8.2:+14452571 None:intergenic
CCAAATTCGCAGAAGCTAGT+TGG 0.511305 8.2:-14453651 MS.gene62450:CDS
ACTAGCTTCTGCGAATTTGG+AGG 0.524749 8.2:+14453654 None:intergenic
GGAAGGGTCATCCTTGGAGG+AGG 0.531078 8.2:+14453801 None:intergenic
GGCGGTGACATCTTAGAGGT+TGG 0.533133 8.2:+14453894 None:intergenic
AATTTACACTTATCTGCATA+GGG 0.538027 8.2:+14453555 None:intergenic
AACAATGACTTCAAAAGAGA+AGG 0.542813 8.2:-14453852 MS.gene62450:CDS
CTCCCCTGCAAGTCAACTTG+TGG 0.543090 8.2:-14452570 MS.gene62450:CDS
AAGTTGACTTGCAGGGGAGG+TGG 0.548779 8.2:+14452574 None:intergenic
AAATTGCAACTTTGCTCATG+GGG 0.550865 8.2:-14453697 MS.gene62450:CDS
AGGAGGGCATATCAATGCAT+TGG 0.555491 8.2:+14453818 None:intergenic
TGAGGAAGGGTCATCCTTGG+AGG 0.556539 8.2:+14453798 None:intergenic
CATTGATATGCCCTCCTCCA+AGG 0.556864 8.2:-14453812 MS.gene62450:CDS
GAATTTACACTTATCTGCAT+AGG 0.559420 8.2:+14453554 None:intergenic
TTTCCACAAGTTGACTTGCA+GGG 0.560920 8.2:+14452567 None:intergenic
TAAGTGCCCCTAAATTGTGC+AGG 0.568865 8.2:+14453519 None:intergenic
TGGTCTGATCTGTTTGATTG+AGG 0.571695 8.2:+14453780 None:intergenic
CTGTGCAAGAAGTATAGCAA+TGG 0.572239 8.2:-14453588 MS.gene62450:CDS
TCAGCTTCAATGGCTCCACC+AGG 0.575223 8.2:+14452632 None:intergenic
CTAAGAAGAAACGTATACTT+GGG 0.583825 8.2:-14452529 MS.gene62450:CDS
AAGAACTGTCACTTTGCTCA+TGG 0.585017 8.2:-14453441 MS.gene62450:CDS
TTCCACAAGTTGACTTGCAG+GGG 0.588906 8.2:+14452568 None:intergenic
AGACTAAGCTGTGTCTCAAG+TGG 0.589149 8.2:-14453491 MS.gene62450:CDS
ATTGTGCAGGATCTTCATGA+AGG 0.599081 8.2:+14453532 None:intergenic
TAAGCTGTGTCTCAAGTGGA+AGG 0.609632 8.2:-14453487 MS.gene62450:CDS
AAAGTGACAGTTCTTTCCAA+AGG 0.613225 8.2:+14453449 None:intergenic
TGATCTGTTTGATTGAGGAA+GGG 0.618914 8.2:+14453785 None:intergenic
GAAGGGTCATCCTTGGAGGA+GGG 0.622595 8.2:+14453802 None:intergenic
AATATCATCTGGAAAAGCCA+TGG 0.624162 8.2:+14453917 None:intergenic
TTCAATGAAGAGAACTGAGG+CGG 0.637828 8.2:+14453876 None:intergenic
GAAGATCCTGCACAATTTAG+GGG 0.650410 8.2:-14453525 MS.gene62450:CDS
CAAGAAGTATAGCAATGGGG+AGG 0.680526 8.2:-14453583 MS.gene62450:CDS
TCAGAGTTACAAGTTCCTGG+TGG 0.718686 8.2:-14452647 MS.gene62450:intron
GTGCAAGAAGTATAGCAATG+GGG 0.763140 8.2:-14453586 MS.gene62450:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAAAAAGTTTTACATT+TGG - chr8.2:14453274-14453293 MS.gene62450:intron 10.0%
!!! GACAAATTTTTTTAAATATA+AGG + chr8.2:14453595-14453614 None:intergenic 10.0%
!!! TTTTTTTAAATATAAGGTCT+AGG + chr8.2:14453589-14453608 None:intergenic 15.0%
!! AACGATTAAATGATTTGAAT+GGG - chr8.2:14453128-14453147 MS.gene62450:intron 20.0%
!!! CAATAACTCATTTTGATTAT+TGG - chr8.2:14452987-14453006 MS.gene62450:intron 20.0%
!!! TACTATAATTTCTAGTAGTT+TGG - chr8.2:14453302-14453321 MS.gene62450:intron 20.0%
!!! TTATAACAATTGCAACTTTT+TGG - chr8.2:14453675-14453694 MS.gene62450:CDS 20.0%
! AATGGATGATTACTATTGTA+TGG - chr8.2:14453239-14453258 MS.gene62450:intron 25.0%
! AATTTACACTTATCTGCATA+GGG + chr8.2:14452841-14452860 None:intergenic 25.0%
! AGTAATCATCCATTAGAATA+AGG + chr8.2:14453233-14453252 None:intergenic 25.0%
! ATTTGTACTAAGTTCAGATT+TGG - chr8.2:14452655-14452674 MS.gene62450:intron 25.0%
! CAACGATTAAATGATTTGAA+TGG - chr8.2:14453127-14453146 MS.gene62450:intron 25.0%
!! ACTTTAAGAAGAAACAAGTT+TGG + chr8.2:14453377-14453396 None:intergenic 25.0%
!! TTAAAGTTTCTGCTGATTAT+TGG - chr8.2:14453390-14453409 MS.gene62450:intron 25.0%
!! TTCGTGTTTTGTAGAAAATA+TGG + chr8.2:14452636-14452655 None:intergenic 25.0%
!!! CTCATTTTGATTATTGGTTT+TGG - chr8.2:14452993-14453012 MS.gene62450:intron 25.0%
AACAATGACTTCAAAAGAGA+AGG - chr8.2:14452541-14452560 MS.gene62450:CDS 30.0%
AAGAGTCAGAACAATATACA+TGG + chr8.2:14453704-14453723 None:intergenic 30.0%
AGTCAGATCAATCGTATTTA+CGG - chr8.2:14453896-14453915 MS.gene62450:CDS 30.0%
ATCATCCATTAGAATAAGGA+TGG + chr8.2:14453229-14453248 None:intergenic 30.0%
CCTCAAAATTATTGATGAGT+CGG + chr8.2:14453431-14453450 None:intergenic 30.0%
CTAAGAAGAAACGTATACTT+GGG - chr8.2:14453864-14453883 MS.gene62450:CDS 30.0%
GAATTTACACTTATCTGCAT+AGG + chr8.2:14452842-14452861 None:intergenic 30.0%
GATGAACATAATACAATAGC+TGG + chr8.2:14453350-14453369 None:intergenic 30.0%
TGATTCCATCCTTATTCTAA+TGG - chr8.2:14453221-14453240 MS.gene62450:intron 30.0%
!! TTTTTCAATGAAGAGAACTG+AGG + chr8.2:14452523-14452542 None:intergenic 30.0%
!!! AGGGTTTGTTGAATTTTCTA+TGG + chr8.2:14453798-14453817 None:intergenic 30.0%
AAAGTGACAGTTCTTTCCAA+AGG + chr8.2:14452947-14452966 None:intergenic 35.0%
AAATCAGAGTTACAAGTTCC+TGG - chr8.2:14453743-14453762 MS.gene62450:CDS 35.0%
AAATTGCAACTTTGCTCATG+GGG - chr8.2:14452696-14452715 MS.gene62450:intron 35.0%
ACAAATTGCAACTTTGCTCA+TGG - chr8.2:14452694-14452713 MS.gene62450:intron 35.0%
ATCATACACTTGCACACAAA+TGG + chr8.2:14453617-14453636 None:intergenic 35.0%
ATGAAGATCCTGCACAATTT+AGG - chr8.2:14452866-14452885 MS.gene62450:intron 35.0%
CAAATTGCAACTTTGCTCAT+GGG - chr8.2:14452695-14452714 MS.gene62450:intron 35.0%
CCAAGTATACGTTTCTTCTT+AGG + chr8.2:14453866-14453885 None:intergenic 35.0%
CCTAAGAAGAAACGTATACT+TGG - chr8.2:14453863-14453882 MS.gene62450:CDS 35.0%
GATACAGGATATTGTTCCTT+TGG - chr8.2:14452928-14452947 MS.gene62450:intron 35.0%
TCAATCGTATTTACGGTGAT+TGG - chr8.2:14453903-14453922 MS.gene62450:CDS 35.0%
TGAAGATCCTGCACAATTTA+GGG - chr8.2:14452867-14452886 MS.gene62450:intron 35.0%
TGATCTGTTTGATTGAGGAA+GGG + chr8.2:14452611-14452630 None:intergenic 35.0%
TGTCTTCATCATCACAAATG+CGG + chr8.2:14453532-14453551 None:intergenic 35.0%
TGTTCTGACTCTTGAAGTAT+TGG - chr8.2:14453711-14453730 MS.gene62450:CDS 35.0%
TTAGAGGTTGGAATATCATC+TGG + chr8.2:14452490-14452509 None:intergenic 35.0%
! CCGACTCATCAATAATTTTG+AGG - chr8.2:14453428-14453447 MS.gene62450:intron 35.0%
! TGTGCAAGAAGTATAGCAAT+GGG - chr8.2:14452806-14452825 MS.gene62450:intron 35.0%
!! AGATTTGGTGCTTGTAGAAA+CGG - chr8.2:14452670-14452689 MS.gene62450:intron 35.0%
!! TTGACAAAGCAAGTTCTGAA+TGG - chr8.2:14453194-14453213 MS.gene62450:intron 35.0%
!!! TTTGAATGGGTGTCATTTTG+TGG - chr8.2:14453141-14453160 MS.gene62450:intron 35.0%
AATTGCAGCTTCAGCTTCAA+TGG + chr8.2:14453774-14453793 None:intergenic 40.0%
ATTGTGCAGGATCTTCATGA+AGG + chr8.2:14452864-14452883 None:intergenic 40.0%
CTGATCTGTTTGATTGAGGA+AGG + chr8.2:14452612-14452631 None:intergenic 40.0%
CTTAATACCGCGGTCAAATA+AGG - chr8.2:14453497-14453516 MS.gene62450:CDS 40.0%
CTTATTTGACCGCGGTATTA+AGG + chr8.2:14453499-14453518 None:intergenic 40.0%
GAAGATCCTGCACAATTTAG+GGG - chr8.2:14452868-14452887 MS.gene62450:intron 40.0%
TGCAAGTCAACTTGTGGAAA+AGG - chr8.2:14453829-14453848 MS.gene62450:CDS 40.0%
TGGTCTGATCTGTTTGATTG+AGG + chr8.2:14452616-14452635 None:intergenic 40.0%
TTCAATGAAGAGAACTGAGG+CGG + chr8.2:14452520-14452539 None:intergenic 40.0%
! CTGTGCAAGAAGTATAGCAA+TGG - chr8.2:14452805-14452824 MS.gene62450:intron 40.0%
! GCATTAACCTTATTTGACCG+CGG + chr8.2:14453507-14453526 None:intergenic 40.0%
! GTGCAAGAAGTATAGCAATG+GGG - chr8.2:14452807-14452826 MS.gene62450:intron 40.0%
!! AAGAACTGTCACTTTGCTCA+TGG - chr8.2:14452952-14452971 MS.gene62450:intron 40.0%
!! CACAATTTAGGGGCACTTAT+CGG - chr8.2:14452878-14452897 MS.gene62450:intron 40.0%
!! TTTCCACAAGTTGACTTGCA+GGG + chr8.2:14453829-14453848 None:intergenic 40.0%
!!! TTTTCCACAAGTTGACTTGC+AGG + chr8.2:14453830-14453849 None:intergenic 40.0%
AGACTAAGCTGTGTCTCAAG+TGG - chr8.2:14452902-14452921 MS.gene62450:intron 45.0%
CACTTTGCTCATGGTGAAGA+AGG - chr8.2:14452961-14452980 MS.gene62450:intron 45.0%
CCAAATTCGCAGAAGCTAGT+TGG - chr8.2:14452742-14452761 MS.gene62450:intron 45.0%
TAAGCTGTGTCTCAAGTGGA+AGG - chr8.2:14452906-14452925 MS.gene62450:intron 45.0%
TAAGTGCCCCTAAATTGTGC+AGG + chr8.2:14452877-14452896 None:intergenic 45.0%
TCAGAGTTACAAGTTCCTGG+TGG - chr8.2:14453746-14453765 MS.gene62450:CDS 45.0%
TGTCTCAAGTGGAAGGATAC+AGG - chr8.2:14452913-14452932 MS.gene62450:intron 45.0%
! ACTAGCTTCTGCGAATTTGG+AGG + chr8.2:14452742-14452761 None:intergenic 45.0%
! AGGAGGGCATATCAATGCAT+TGG + chr8.2:14452578-14452597 None:intergenic 45.0%
! CAAGAAGTATAGCAATGGGG+AGG - chr8.2:14452810-14452829 MS.gene62450:intron 45.0%
! CCAACTAGCTTCTGCGAATT+TGG + chr8.2:14452745-14452764 None:intergenic 45.0%
!! TTCCACAAGTTGACTTGCAG+GGG + chr8.2:14453828-14453847 None:intergenic 45.0%
AGCTTCTGCGAATTTGGAGG+TGG + chr8.2:14452739-14452758 None:intergenic 50.0%
CATTGATATGCCCTCCTCCA+AGG - chr8.2:14452581-14452600 MS.gene62450:CDS 50.0%
CGGTATTAAGGATCGTGGCA+TGG + chr8.2:14453487-14453506 None:intergenic 50.0%
CTCATGGGGCTGAAGAGATA+AGG - chr8.2:14452710-14452729 MS.gene62450:intron 50.0%
GATTGAGGAAGGGTCATCCT+TGG + chr8.2:14452601-14452620 None:intergenic 50.0%
TGCCACGATCCTTAATACCG+CGG - chr8.2:14453487-14453506 MS.gene62450:CDS 50.0%
CTCCCCTGCAAGTCAACTTG+TGG - chr8.2:14453823-14453842 MS.gene62450:CDS 55.0%
CTGAGGCGGTGACATCTTAG+AGG + chr8.2:14452506-14452525 None:intergenic 55.0%
GAAGGGTCATCCTTGGAGGA+GGG + chr8.2:14452594-14452613 None:intergenic 55.0%
GACCGCGGTATTAAGGATCG+TGG + chr8.2:14453492-14453511 None:intergenic 55.0%
GGCGGTGACATCTTAGAGGT+TGG + chr8.2:14452502-14452521 None:intergenic 55.0%
TCAGCTTCAATGGCTCCACC+AGG + chr8.2:14453764-14453783 None:intergenic 55.0%
TGAGGAAGGGTCATCCTTGG+AGG + chr8.2:14452598-14452617 None:intergenic 55.0%
! AAGGTCTAGGACCACGACTG+TGG + chr8.2:14453576-14453595 None:intergenic 55.0%
!! AAGTTGACTTGCAGGGGAGG+TGG + chr8.2:14453822-14453841 None:intergenic 55.0%
!! CACAAGTTGACTTGCAGGGG+AGG + chr8.2:14453825-14453844 None:intergenic 55.0%
GACTTGCAGGGGAGGTGGTA+GGG + chr8.2:14453817-14453836 None:intergenic 60.0%
GGAAGGGTCATCCTTGGAGG+AGG + chr8.2:14452595-14452614 None:intergenic 60.0%
GTACGTCTCAGCCACAGTCG+TGG - chr8.2:14453562-14453581 MS.gene62450:CDS 60.0%
! TGACTTGCAGGGGAGGTGGT+AGG + chr8.2:14453818-14453837 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 14452478 14453937 14452478 ID=MS.gene62450
chr8.2 mRNA 14452478 14453937 14452478 ID=MS.gene62450.t1;Parent=MS.gene62450
chr8.2 exon 14453433 14453937 14453433 ID=MS.gene62450.t1.exon1;Parent=MS.gene62450.t1
chr8.2 CDS 14453433 14453937 14453433 ID=cds.MS.gene62450.t1;Parent=MS.gene62450.t1
chr8.2 exon 14452478 14452665 14452478 ID=MS.gene62450.t1.exon2;Parent=MS.gene62450.t1
chr8.2 CDS 14452478 14452665 14452478 ID=cds.MS.gene62450.t1;Parent=MS.gene62450.t1
Gene Sequence

>MS.gene62450

ATGGCTTTTCCAGATGATATTCCAACCTCTAAGATGTCACCGCCTCAGTTCTCTTCATTGAAAAACAATGACTTCAAAAGAGAAGGAACTTCTCATTCCAATGCATTGATATGCCCTCCTCCAAGGATGACCCTTCCTCAATCAAACAGATCAGACCATATTTTCTACAAAACACGAATTTGTACTAAGTTCAGATTTGGTGCTTGTAGAAACGGAACAAATTGCAACTTTGCTCATGGGGCTGAAGAGATAAGGAAACCACCTCCAAATTCGCAGAAGCTAGTTGGTCTGTGCAACGAAGATAAAAAAATCATAAACAAGATGAAACTGTGCAAGAAGTATAGCAATGGGGAGGAATGTCCCTATGCAGATAAGTGTAAATTCCTTCATGAAGATCCTGCACAATTTAGGGGCACTTATCGGAAGACTAAGCTGTGTCTCAAGTGGAAGGATACAGGATATTGTTCCTTTGGAAAGAACTGTCACTTTGCTCATGGTGAAGAAGGTAACAATAACTCATTTTGATTATTGGTTTTGGTTTTATCATATAATTCTACTGTTTTATATTGTTCAATCATATAAAATATGTTTTTTTAATTTTCTAGATGCTGTGCAGAATCTCATTTTATGTGATATGATGTGCAAATTACAACGATTAAATGATTTGAATGGGTGTCATTTTGTGGTTGCGTTGTGTATTATGTTTCATTTTTTATTTGACAAAGCAAGTTCTGAATGGAAAATGATTCCATCCTTATTCTAATGGATGATTACTATTGTATGGTGTTAAAAGTTAAAAAAAAAAAGTTTTACATTTGGTAAGATACTATAATTTCTAGTAGTTTGGAGTAAGTTGCGATTCTTAATAACCAGCTATTGTATTATGTTCATCTTTTCCAAACTTGTTTCTTCTTAAAGTTTCTGCTGATTATTGGCTATAAATTTGATGTCCGACTCATCAATAATTTTGAGGTGCGTTGCTCATTTGCAAAGTTTTAAATTGTGACCATGCCACGATCCTTAATACCGCGGTCAAATAAGGTTAATGCAGCCGCATTTGTGATGATGAAGACATAAGACATTTGTACGTCTCAGCCACAGTCGTGGTCCTAGACCTTATATTTAAAAAAATTTGTCCATTTGTGTGCAAGTGTATGATAACTTATATTTGCATATTGTGAGCAAAATTACAGATGATTATAACAATTGCAACTTTTTGGTTTCCATGTATATTGTTCTGACTCTTGAAGTATTGGTTGTCTTGCAAATCAGAGTTACAAGTTCCTGGTGGAGCCATTGAAGCTGAAGCTGCAATTACCATAGAAAATTCAACAAACCCTACCACCTCCCCTGCAAGTCAACTTGTGGAAAAGGAAGAAGAGCAGCCTAAGAAGAAACGTATACTTGGGTTGAAATTCAGTCAGATCAATCGTATTTACGGTGATTGGATTGACGATTGA
Protein sequence

>MS.gene62450.t1

MAFPDDIPTSKMSPPQFSSLKNNDFKREGTSHSNALICPPPRMTLPQSNRSDHIFYKTRICTKFRFGACRNGTNCNFAHGAEEIRKPPPNSQKLVGLCNEDKKIINKMKLCKKYSNGEECPYADKCKFLHEDPAQFRGTYRKTKLCLKWKDTGYCSFGKNCHFAHGEEELQVPGGAIEAEAAITIENSTNPTTSPASQLVEKEEEQPKKKRILGLKFSQINRIYGDWIDD