Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62538.t1 | XP_013452170.1 | 65 | 200 | 46 | 6 | 1 | 187 | 1 | 189 | 1.90E-49 | 205.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62538.t1 | A0A072UAR9 | 65.0 | 200 | 46 | 6 | 1 | 187 | 1 | 189 | 1.4e-49 | 205.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene62538.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62538.t1 | MTR_6g047570 | 65.000 | 200 | 46 | 6 | 1 | 187 | 1 | 189 | 1.06e-72 | 223 |
| MS.gene62538.t1 | MTR_6g047550 | 61.215 | 214 | 45 | 7 | 1 | 187 | 1 | 203 | 8.55e-71 | 216 |
| MS.gene62538.t1 | MTR_0250s0040 | 65.854 | 164 | 10 | 2 | 24 | 187 | 1 | 118 | 2.11e-66 | 204 |
| MS.gene62538.t1 | MTR_0011s0260 | 58.333 | 216 | 58 | 7 | 1 | 187 | 1 | 213 | 1.47e-55 | 179 |
| MS.gene62538.t1 | MTR_4g066460 | 58.000 | 200 | 56 | 7 | 1 | 187 | 1 | 185 | 1.66e-55 | 178 |
| MS.gene62538.t1 | MTR_0011s0210 | 58.333 | 216 | 58 | 7 | 1 | 187 | 1 | 213 | 2.18e-55 | 179 |
| MS.gene62538.t1 | MTR_4g067010 | 58.247 | 194 | 57 | 7 | 1 | 187 | 1 | 177 | 3.11e-53 | 172 |
| MS.gene62538.t1 | MTR_4g066380 | 53.171 | 205 | 56 | 8 | 1 | 187 | 1 | 183 | 8.40e-53 | 171 |
| MS.gene62538.t1 | MTR_0246s0020 | 41.949 | 236 | 80 | 7 | 1 | 187 | 1 | 228 | 1.31e-40 | 141 |
| MS.gene62538.t1 | MTR_0246s0020 | 41.949 | 236 | 80 | 7 | 1 | 187 | 1 | 228 | 1.80e-40 | 141 |
| MS.gene62538.t1 | MTR_4g098035 | 40.654 | 214 | 84 | 12 | 1 | 185 | 1 | 200 | 6.26e-25 | 99.4 |
| MS.gene62538.t1 | MTR_0246s0050 | 38.220 | 191 | 77 | 10 | 23 | 185 | 2 | 179 | 4.83e-18 | 80.5 |
| MS.gene62538.t1 | MTR_5g014600 | 34.043 | 188 | 95 | 6 | 14 | 187 | 7 | 179 | 2.33e-16 | 75.9 |
| MS.gene62538.t1 | MTR_5g014560 | 30.556 | 180 | 82 | 6 | 18 | 187 | 15 | 161 | 1.27e-11 | 62.4 |
| MS.gene62538.t1 | MTR_2g010450 | 50.877 | 57 | 25 | 1 | 134 | 187 | 151 | 207 | 9.61e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATTTATAGTGGATGAATT+TGG | 0.185655 | 7.3:-69739035 | None:intergenic |
| AATTTATAGTGGATGAATTT+GGG | 0.217326 | 7.3:-69739034 | None:intergenic |
| TCTTGTTCCTAACCTTAAAA+AGG | 0.267726 | 7.3:+69739250 | MS.gene62538:CDS |
| CAACAGCTGGTTATCTGATT+TGG | 0.283101 | 7.3:+69737498 | MS.gene62538:CDS |
| TCCACATCTATAAGGAAATT+CGG | 0.302534 | 7.3:-69738822 | None:intergenic |
| GATGAGTTGGAAGAAAGTTT+TGG | 0.324709 | 7.3:-69738978 | None:intergenic |
| ATAAATTCCTCACAATTTAG+TGG | 0.347904 | 7.3:+69739050 | MS.gene62538:CDS |
| CAGAAGAGTCATCGAGAAAA+AGG | 0.350658 | 7.3:+69739141 | MS.gene62538:CDS |
| GAAGCTCTTCCACATCTATA+AGG | 0.379411 | 7.3:-69738830 | None:intergenic |
| AGCTGTGTCTCCAACTGAAA+TGG | 0.401088 | 7.3:+69739091 | MS.gene62538:CDS |
| CCACATCTATAAGGAAATTC+GGG | 0.402643 | 7.3:-69738821 | None:intergenic |
| ATAAACAATGTTTGACTTCG+AGG | 0.433014 | 7.3:+69738892 | MS.gene62538:CDS |
| TTGAAACTGAAAAGATGAGT+TGG | 0.465335 | 7.3:-69738991 | None:intergenic |
| AAATTGTGAGGAATTTATAG+TGG | 0.484308 | 7.3:-69739045 | None:intergenic |
| AAAAGGTCTCGTGGTAATCA+TGG | 0.502601 | 7.3:+69739158 | MS.gene62538:CDS |
| ATTTCAGTTGGAGACACAGC+TGG | 0.503534 | 7.3:-69739089 | None:intergenic |
| CCCGAATTTCCTTATAGATG+TGG | 0.531562 | 7.3:+69738821 | MS.gene62538:CDS |
| TTCAAATCCACTAAATTGTG+AGG | 0.531739 | 7.3:-69739057 | None:intergenic |
| CGAATACAATTTAATTGCTG+AGG | 0.543999 | 7.3:+69738791 | MS.gene62538:CDS |
| TCATGGTTATGATCATATCA+TGG | 0.552683 | 7.3:+69739175 | MS.gene62538:CDS |
| CAAATCAGATAACCAGCTGT+TGG | 0.564229 | 7.3:-69737497 | None:intergenic |
| TTTGGGGTGTTGTCAAAACA+TGG | 0.585137 | 7.3:-69739017 | None:intergenic |
| ATTTATAGTGGATGAATTTG+GGG | 0.591446 | 7.3:-69739033 | None:intergenic |
| TATCATCATCAACCAACAGC+TGG | 0.596947 | 7.3:+69737485 | MS.gene62538:CDS |
| GTTGCTGAGAGTTGTGTGAG+TGG | 0.604875 | 7.3:-69738916 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAATTTATATCTTTAACA+TGG | - | chr7.3:69738396-69738415 | None:intergenic | 10.0% |
| !! | CTTGAAAATAATTTCAATTA+GGG | + | chr7.3:69737667-69737686 | MS.gene62538:intron | 15.0% |
| !! | TTGTTTGTAATATGAATATT+TGG | + | chr7.3:69738230-69738249 | MS.gene62538:intron | 15.0% |
| !!! | AAAGGAAATTGATTTTATAT+TGG | + | chr7.3:69738192-69738211 | MS.gene62538:intron | 15.0% |
| !! | AAATGTTCTTGTTAATGTTA+TGG | - | chr7.3:69737948-69737967 | None:intergenic | 20.0% |
| !! | ATAATCATTTGATACATCAT+TGG | - | chr7.3:69738057-69738076 | None:intergenic | 20.0% |
| !! | ATTATATATATTAACCCACT+TGG | + | chr7.3:69738072-69738091 | MS.gene62538:intron | 20.0% |
| !! | CATTTGTGAATTTAGAAAAT+AGG | - | chr7.3:69737553-69737572 | None:intergenic | 20.0% |
| !! | CATTTGTGAATTTCAAAAAT+AGG | - | chr7.3:69738686-69738705 | None:intergenic | 20.0% |
| !! | CTCCAAAAAAATCATATTAA+GGG | - | chr7.3:69738294-69738313 | None:intergenic | 20.0% |
| !! | GCTTGAAAATAATTTCAATT+AGG | + | chr7.3:69737666-69737685 | MS.gene62538:intron | 20.0% |
| !! | TAATCATTTGATACATCATT+GGG | - | chr7.3:69738056-69738075 | None:intergenic | 20.0% |
| !! | TATGAATATTTGGCATAATT+AGG | + | chr7.3:69738240-69738259 | MS.gene62538:intron | 20.0% |
| !! | TCTCCAAAAAAATCATATTA+AGG | - | chr7.3:69738295-69738314 | None:intergenic | 20.0% |
| !!! | AATTTATAGTGGATGAATTT+GGG | - | chr7.3:69739037-69739056 | None:intergenic | 20.0% |
| !!! | ATTGTAGAAGTATTATACAT+AGG | - | chr7.3:69738340-69738359 | None:intergenic | 20.0% |
| !!! | ATTGTTTATTGTTTTGTTCT+TGG | - | chr7.3:69738881-69738900 | None:intergenic | 20.0% |
| !!! | CTATTTTCTAAATTCACAAA+TGG | + | chr7.3:69737551-69737570 | MS.gene62538:intron | 20.0% |
| !!! | CTATTTTTGAAATTCACAAA+TGG | + | chr7.3:69738684-69738703 | MS.gene62538:intron | 20.0% |
| !!! | CTTATGGTTTTTAGAATATA+AGG | - | chr7.3:69738493-69738512 | None:intergenic | 20.0% |
| !!! | TGTTAGTATGACTTTTAAAA+AGG | + | chr7.3:69738174-69738193 | MS.gene62538:intron | 20.0% |
| !!! | TTGCTGAAACATTATATTTT+AGG | - | chr7.3:69737984-69738003 | None:intergenic | 20.0% |
| !!! | TTGTTTATTGTTTTGTTCTT+GGG | - | chr7.3:69738880-69738899 | None:intergenic | 20.0% |
| !!! | TTTTAGAATATAAGGTAACA+TGG | - | chr7.3:69738485-69738504 | None:intergenic | 20.0% |
| ! | AAATTGTGAGGAATTTATAG+TGG | - | chr7.3:69739048-69739067 | None:intergenic | 25.0% |
| ! | ATAAATTCCTCACAATTTAG+TGG | + | chr7.3:69739050-69739069 | MS.gene62538:CDS | 25.0% |
| ! | ATTTAGCAAGTTAAAAAGTC+TGG | + | chr7.3:69737740-69737759 | MS.gene62538:intron | 25.0% |
| ! | GTGAATTTAGAAAATAGGAA+GGG | - | chr7.3:69737548-69737567 | None:intergenic | 25.0% |
| ! | GTGAATTTCAAAAATAGGAA+GGG | - | chr7.3:69738681-69738700 | None:intergenic | 25.0% |
| ! | GTTAATGTTATGGAATGTTT+AGG | - | chr7.3:69737938-69737957 | None:intergenic | 25.0% |
| ! | TGTGAATTTAGAAAATAGGA+AGG | - | chr7.3:69737549-69737568 | None:intergenic | 25.0% |
| ! | TGTGAATTTCAAAAATAGGA+AGG | - | chr7.3:69738682-69738701 | None:intergenic | 25.0% |
| !! | TTCATATTTTCCATTTCAGT+TGG | - | chr7.3:69739104-69739123 | None:intergenic | 25.0% |
| !!! | ATTTATAGTGGATGAATTTG+GGG | - | chr7.3:69739036-69739055 | None:intergenic | 25.0% |
| !!! | CTCCCTTAATATGATTTTTT+TGG | + | chr7.3:69738289-69738308 | MS.gene62538:intron | 25.0% |
| !!! | GAATTTATAGTGGATGAATT+TGG | - | chr7.3:69739038-69739057 | None:intergenic | 25.0% |
| !!! | TATTCTACCTTTTTAAGGTT+AGG | - | chr7.3:69739260-69739279 | None:intergenic | 25.0% |
| !!! | TCTTAAGTTTTTCAGATAGA+AGG | - | chr7.3:69737876-69737895 | None:intergenic | 25.0% |
| AATGTTATGGAATGTTTAGG+AGG | - | chr7.3:69737935-69737954 | None:intergenic | 30.0% | |
| AATTTCAAAAATAGGAAGGG+TGG | - | chr7.3:69738678-69738697 | None:intergenic | 30.0% | |
| ACATATTTCCGAATACAAAG+TGG | - | chr7.3:69737709-69737728 | None:intergenic | 30.0% | |
| AGATGCATTAGTTATGCATA+AGG | - | chr7.3:69738271-69738290 | None:intergenic | 30.0% | |
| ATATATATTAACCCACTTGG+CGG | + | chr7.3:69738075-69738094 | MS.gene62538:intron | 30.0% | |
| ATATTTCCGAATACAAAGTG+GGG | - | chr7.3:69737707-69737726 | None:intergenic | 30.0% | |
| ATGTTATGGAATGTTTAGGA+GGG | - | chr7.3:69737934-69737953 | None:intergenic | 30.0% | |
| CATATTTCCGAATACAAAGT+GGG | - | chr7.3:69737708-69737727 | None:intergenic | 30.0% | |
| CATTAGTTATGCATAAGGAA+AGG | - | chr7.3:69738266-69738285 | None:intergenic | 30.0% | |
| CGAATACAATTTAATTGCTG+AGG | + | chr7.3:69738791-69738810 | MS.gene62538:CDS | 30.0% | |
| TCATACTAACACATCGAAAT+TGG | - | chr7.3:69738165-69738184 | None:intergenic | 30.0% | |
| TCATGGTTATGATCATATCA+TGG | + | chr7.3:69739175-69739194 | MS.gene62538:CDS | 30.0% | |
| TCCACATCTATAAGGAAATT+CGG | - | chr7.3:69738825-69738844 | None:intergenic | 30.0% | |
| TCTTGTTCCTAACCTTAAAA+AGG | + | chr7.3:69739250-69739269 | MS.gene62538:CDS | 30.0% | |
| TTCAAATCCACTAAATTGTG+AGG | - | chr7.3:69739060-69739079 | None:intergenic | 30.0% | |
| TTGAAACTGAAAAGATGAGT+TGG | - | chr7.3:69738994-69739013 | None:intergenic | 30.0% | |
| ! | ATAAACAATGTTTGACTTCG+AGG | + | chr7.3:69738892-69738911 | MS.gene62538:CDS | 30.0% |
| ! | GAAATCAAAGCTACTTTCAA+AGG | - | chr7.3:69737904-69737923 | None:intergenic | 30.0% |
| ! | GATTTGGTAAGCAAATCAAA+AGG | + | chr7.3:69737514-69737533 | MS.gene62538:intron | 30.0% |
| !! | TAAGAAAGCATTTGTATGTG+AGG | - | chr7.3:69737828-69737847 | None:intergenic | 30.0% |
| !!! | CTTGAAATTTCCTTTTTTGC+AGG | + | chr7.3:69738761-69738780 | MS.gene62538:intron | 30.0% |
| AGAAACCTTGCCTCATATTT+AGG | + | chr7.3:69738312-69738331 | MS.gene62538:intron | 35.0% | |
| CCACATCTATAAGGAAATTC+GGG | - | chr7.3:69738824-69738843 | None:intergenic | 35.0% | |
| GACTATAGAGTGAGTGTATT+AGG | + | chr7.3:69738122-69738141 | MS.gene62538:intron | 35.0% | |
| TACATAGGAGCCTAAATATG+AGG | - | chr7.3:69738325-69738344 | None:intergenic | 35.0% | |
| TAGAAGGAGAAATGCAACAA+AGG | - | chr7.3:69738522-69738541 | None:intergenic | 35.0% | |
| TCGTTCATTTCCTGCAAAAA+AGG | - | chr7.3:69738774-69738793 | None:intergenic | 35.0% | |
| TTGTTGCATTTCTCCTTCTA+GGG | + | chr7.3:69738522-69738541 | MS.gene62538:intron | 35.0% | |
| TTTGTTGCATTTCTCCTTCT+AGG | + | chr7.3:69738521-69738540 | MS.gene62538:intron | 35.0% | |
| ! | GAAGGGTGGATCATATATTT+TGG | - | chr7.3:69738664-69738683 | None:intergenic | 35.0% |
| ! | GTTTTACCCCACTTTGTATT+CGG | + | chr7.3:69737698-69737717 | MS.gene62538:intron | 35.0% |
| !! | GATGAGTTGGAAGAAAGTTT+TGG | - | chr7.3:69738981-69739000 | None:intergenic | 35.0% |
| !! | GGAAGAAAGTTTTGGTGAAA+AGG | - | chr7.3:69738973-69738992 | None:intergenic | 35.0% |
| AAAAGGTCTCGTGGTAATCA+TGG | + | chr7.3:69739158-69739177 | MS.gene62538:CDS | 40.0% | |
| CAAATCAGATAACCAGCTGT+TGG | - | chr7.3:69737500-69737519 | None:intergenic | 40.0% | |
| CAACAGCTGGTTATCTGATT+TGG | + | chr7.3:69737498-69737517 | MS.gene62538:CDS | 40.0% | |
| CAGAAGAGTCATCGAGAAAA+AGG | + | chr7.3:69739141-69739160 | MS.gene62538:CDS | 40.0% | |
| CCATTATGAGCAAGTTTGGT+TGG | - | chr7.3:69738553-69738572 | None:intergenic | 40.0% | |
| CCCGAATTTCCTTATAGATG+TGG | + | chr7.3:69738821-69738840 | MS.gene62538:CDS | 40.0% | |
| GAAGCTCTTCCACATCTATA+AGG | - | chr7.3:69738833-69738852 | None:intergenic | 40.0% | |
| GCAACAAAGGACATGACTTA+TGG | - | chr7.3:69738509-69738528 | None:intergenic | 40.0% | |
| TATCATCATCAACCAACAGC+TGG | + | chr7.3:69737485-69737504 | MS.gene62538:CDS | 40.0% | |
| TTGGCCATTATGAGCAAGTT+TGG | - | chr7.3:69738557-69738576 | None:intergenic | 40.0% | |
| ! | CCAACCAAACTTGCTCATAA+TGG | + | chr7.3:69738550-69738569 | MS.gene62538:intron | 40.0% |
| ! | TTTTCTCGATGACTCTTCTG+AGG | - | chr7.3:69739141-69739160 | None:intergenic | 40.0% |
| !! | AATTTTAACGTCCCGTGCAA+CGG | - | chr7.3:69737604-69737623 | None:intergenic | 40.0% |
| !! | TTTGGGGTGTTGTCAAAACA+TGG | - | chr7.3:69739020-69739039 | None:intergenic | 40.0% |
| ACACATCGAAATTGGCACTG+AGG | - | chr7.3:69738157-69738176 | None:intergenic | 45.0% | |
| AGCTGTGTCTCCAACTGAAA+TGG | + | chr7.3:69739091-69739110 | MS.gene62538:CDS | 45.0% | |
| AGGAGCCTAAATATGAGGCA+AGG | - | chr7.3:69738320-69738339 | None:intergenic | 45.0% | |
| ATTTCAGTTGGAGACACAGC+TGG | - | chr7.3:69739092-69739111 | None:intergenic | 45.0% | |
| CCTTCTGCACTCTTTATGCT+GGG | - | chr7.3:69737770-69737789 | None:intergenic | 45.0% | |
| TAGTATGAGTACCCGTTGCA+CGG | + | chr7.3:69737589-69737608 | MS.gene62538:intron | 45.0% | |
| TCATCGAGAAAAAGGTCTCG+TGG | + | chr7.3:69739149-69739168 | MS.gene62538:CDS | 45.0% | |
| TTGGTTGGATTTGCCCTAGA+AGG | - | chr7.3:69738538-69738557 | None:intergenic | 45.0% | |
| !! | ATTTTAACGTCCCGTGCAAC+GGG | - | chr7.3:69737603-69737622 | None:intergenic | 45.0% |
| !!! | TTACAATTTTTTTAATATAT+TGG | - | chr7.3:69738576-69738595 | None:intergenic | 5.0% |
| AGTATGAGTACCCGTTGCAC+GGG | + | chr7.3:69737590-69737609 | MS.gene62538:intron | 50.0% | |
| ATCAGACTAAGCCGCCAAGT+GGG | - | chr7.3:69738089-69738108 | None:intergenic | 50.0% | |
| GCCTTCTGCACTCTTTATGC+TGG | - | chr7.3:69737771-69737790 | None:intergenic | 50.0% | |
| GTTGCTGAGAGTTGTGTGAG+TGG | - | chr7.3:69738919-69738938 | None:intergenic | 50.0% | |
| TATCAGACTAAGCCGCCAAG+TGG | - | chr7.3:69738090-69738109 | None:intergenic | 50.0% | |
| ! | CCCAGCATAAAGAGTGCAGA+AGG | + | chr7.3:69737767-69737786 | MS.gene62538:intron | 50.0% |
| ! | GCGGCTTAGTCTGATAGTGT+TGG | + | chr7.3:69738094-69738113 | MS.gene62538:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 69737454 | 69739280 | 69737454 | ID=MS.gene62538 |
| chr7.3 | mRNA | 69737454 | 69739280 | 69737454 | ID=MS.gene62538.t1;Parent=MS.gene62538 |
| chr7.3 | exon | 69737454 | 69737519 | 69737454 | ID=MS.gene62538.t1.exon1;Parent=MS.gene62538.t1 |
| chr7.3 | CDS | 69737454 | 69737519 | 69737454 | ID=cds.MS.gene62538.t1;Parent=MS.gene62538.t1 |
| chr7.3 | exon | 69738783 | 69739280 | 69738783 | ID=MS.gene62538.t1.exon2;Parent=MS.gene62538.t1 |
| chr7.3 | CDS | 69738783 | 69739280 | 69738783 | ID=cds.MS.gene62538.t1;Parent=MS.gene62538.t1 |
| Gene Sequence |
| Protein sequence |
