AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62678

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62678.t1 MTR_8g105600 77.008 635 75 9 1 620 1 579 0.0 902
MS.gene62678.t1 MTR_6g016850 88.889 171 18 1 1 170 1 171 1.39e-104 313
MS.gene62678.t1 MTR_4g021760 40.964 249 118 3 9 257 11 230 3.63e-57 195
MS.gene62678.t1 MTR_4g021790 41.860 258 120 4 1 257 1 229 1.85e-55 189
MS.gene62678.t1 MTR_4g021845 40.329 243 116 3 3 245 4 217 1.41e-51 177
MS.gene62678.t1 MTR_1g032570 40.075 267 131 6 8 256 10 265 5.52e-51 186
MS.gene62678.t1 MTR_4g021855 39.526 253 124 3 9 261 10 233 1.49e-50 177
MS.gene62678.t1 MTR_3g086100 38.372 258 148 6 8 260 12 263 2.42e-47 175
MS.gene62678.t1 MTR_2g034960 39.600 250 140 6 7 253 27 268 1.01e-45 171
MS.gene62678.t1 MTR_8g079940 37.500 264 138 5 9 260 25 273 4.30e-45 168
MS.gene62678.t1 MTR_4g121020 40.625 256 131 8 7 253 29 272 9.54e-45 169
MS.gene62678.t1 MTR_4g121020 38.697 261 129 6 7 253 29 272 9.82e-45 169
MS.gene62678.t1 MTR_1g013180 35.714 322 189 8 7 316 154 469 3.76e-44 168
MS.gene62678.t1 MTR_3g106220 38.148 270 126 7 6 253 5 255 8.22e-43 164
MS.gene62678.t1 MTR_6g045327 38.750 240 114 6 14 251 19 227 4.83e-42 156
MS.gene62678.t1 MTR_5g055260 36.948 249 101 4 9 257 10 202 2.15e-41 150
MS.gene62678.t1 MTR_1g013170 33.754 317 193 7 7 310 3 315 3.10e-41 157
MS.gene62678.t1 MTR_7g117705 33.882 304 179 8 5 296 1 294 4.62e-41 155
MS.gene62678.t1 MTR_8g093040 52.593 135 62 2 4 137 9 142 6.25e-40 147
MS.gene62678.t1 MTR_2g450070 36.047 258 151 6 7 253 16 270 3.46e-36 144
MS.gene62678.t1 MTR_2g450070 34.884 258 154 6 7 253 16 270 5.45e-36 144
MS.gene62678.t1 MTR_1g032570 39.130 207 98 5 67 256 1 196 3.19e-35 140
MS.gene62678.t1 MTR_4g131600 35.223 247 150 5 5 249 16 254 3.75e-35 140
MS.gene62678.t1 MTR_4g131570 33.582 268 139 6 5 253 13 260 8.20e-35 139
MS.gene62678.t1 MTR_4g131580 34.100 261 136 6 9 253 17 257 1.08e-34 139
MS.gene62678.t1 MTR_3g086100 38.384 198 112 5 67 260 1 192 4.57e-33 133
MS.gene62678.t1 MTR_3g102590 34.630 257 109 8 1 253 1 202 7.06e-29 115
MS.gene62678.t1 MTR_0450s0040 30.556 252 143 6 11 257 6 230 3.12e-28 116
MS.gene62678.t1 MTR_7g026400 36.598 194 105 5 58 251 37 212 2.49e-25 111
MS.gene62678.t1 MTR_3g102590 33.603 247 107 7 1 245 1 192 2.73e-25 103
MS.gene62678.t1 MTR_4g023980 34.161 161 79 2 54 214 35 168 1.87e-24 100
MS.gene62678.t1 MTR_4g121020 37.222 180 84 4 87 253 5 168 1.99e-24 108
MS.gene62678.t1 MTR_3g102600 36.220 127 79 2 5 130 4 129 2.33e-19 86.7
MS.gene62678.t1 MTR_3g102600 35.938 128 80 2 4 130 3 129 1.21e-18 85.9
MS.gene62678.t1 MTR_3g102600 50.794 63 29 1 191 253 129 189 5.15e-13 69.3
MS.gene62678.t1 MTR_8g032710 27.113 284 154 7 11 260 19 283 2.01e-18 89.0
MS.gene62678.t1 MTR_7g089010 48.684 76 37 1 180 255 77 150 5.89e-14 73.6
MS.gene62678.t1 MTR_4g064730 44.211 95 47 2 187 281 124 212 2.78e-13 71.6
MS.gene62678.t1 MTR_3g102600 50.794 63 29 1 191 253 80 140 5.04e-13 68.2
MS.gene62678.t1 MTR_3g102600 41.096 73 42 1 58 130 9 80 2.24e-12 66.2
MS.gene62678.t1 MTR_3g102600 41.096 73 42 1 58 130 9 80 8.16e-13 66.6
MS.gene62678.t1 MTR_1g013160 30.189 159 83 5 70 214 1 145 1.37e-12 66.2
MS.gene62678.t1 MTR_5g054300 36.036 111 64 2 143 253 153 256 3.21e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62678.t1 AT1G67710 38.462 260 140 5 10 259 8 257 1.15e-47 175
MS.gene62678.t1 AT2G01760 37.218 266 155 6 5 261 3 265 2.19e-45 166
MS.gene62678.t1 AT3G16857 36.397 272 136 8 9 253 33 294 8.40e-41 158
MS.gene62678.t1 AT3G16857 36.397 272 136 8 9 253 33 294 1.35e-40 157
MS.gene62678.t1 AT2G01760 33.673 294 155 7 5 261 3 293 2.43e-39 149
MS.gene62678.t1 AT2G25180 35.458 251 149 5 7 253 11 252 2.69e-39 153
MS.gene62678.t1 AT1G67710 39.604 202 103 4 67 259 1 192 1.18e-35 140
MS.gene62678.t1 AT5G58080 33.136 338 204 8 7 330 12 341 1.60e-35 142
MS.gene62678.t1 AT4G31920 35.081 248 142 5 7 253 11 240 2.80e-35 140
MS.gene62678.t1 AT3G16857 36.910 233 110 8 48 253 2 224 2.64e-32 132
MS.gene62678.t1 AT5G49240 34.127 252 156 7 5 252 34 279 8.66e-32 125
MS.gene62678.t1 AT1G49190 30.363 303 189 8 4 297 25 314 6.01e-31 125
MS.gene62678.t1 AT2G01760 37.438 203 116 5 67 261 1 200 6.23e-31 123
MS.gene62678.t1 AT3G62670 34.855 241 137 8 14 248 42 268 6.52e-29 120
MS.gene62678.t1 AT1G67710 37.363 182 96 3 87 259 11 183 6.24e-28 117
MS.gene62678.t1 AT5G07210 25.746 268 168 7 13 253 16 279 3.45e-17 85.9
MS.gene62678.t1 AT1G49190 33.588 131 85 2 4 133 25 154 6.77e-17 84.7
MS.gene62678.t1 AT1G68210 29.412 272 156 9 8 248 8 274 1.30e-16 84.3
MS.gene62678.t1 AT5G07210 25.000 276 168 8 13 253 16 287 8.07e-15 78.2
MS.gene62678.t1 AT3G10760 53.030 66 29 1 190 255 101 164 9.75e-14 73.2
MS.gene62678.t1 AT5G59570 53.030 66 29 1 190 255 137 200 1.31e-13 72.4
MS.gene62678.t1 AT5G59570 53.030 66 29 1 190 255 137 200 1.31e-13 72.4
MS.gene62678.t1 AT5G05090 47.619 84 31 2 170 253 68 138 2.28e-13 71.2
MS.gene62678.t1 AT2G40970 53.030 66 29 1 190 255 101 164 2.81e-13 70.5
MS.gene62678.t1 AT3G46640 53.030 66 29 1 190 255 140 203 3.04e-13 71.6
MS.gene62678.t1 AT3G46640 53.030 66 29 1 190 255 140 203 3.04e-13 71.6
MS.gene62678.t1 AT3G46640 53.030 66 29 1 190 255 140 203 4.04e-13 71.2

Find 177 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCATCTCTTGCCCTTATT+TGG 0.189913 4.2:+1399978 None:intergenic
TTCTTTGTCCGGGGTGGTTC+GGG 0.278029 4.2:+1399905 None:intergenic
AGCCTGAGCCTGAGGAGCTT+GGG 0.288365 4.2:+1397927 None:intergenic
CTTCTTTGTCCGGGGTGGTT+CGG 0.314627 4.2:+1399904 None:intergenic
GGATTGAAGGTAGCCAAATA+AGG 0.326856 4.2:-1399991 MS.gene62678:CDS
AACGATGTACCATCGGAAAA+AGG 0.331149 4.2:-1399941 MS.gene62678:CDS
AATAATACTGTAACAGATTT+CGG 0.331250 4.2:-1398483 MS.gene62678:CDS
GGCTGGTCCCCAAGCTCCTC+AGG 0.339705 4.2:-1397935 MS.gene62678:CDS
TCTTCTTTGTCTTCTTTGTC+CGG 0.340590 4.2:+1399894 None:intergenic
CAATTAGTATATCTTGTGTT+AGG 0.345112 4.2:+1397829 None:intergenic
ATAATGAAGGCAATTGAAAA+TGG 0.347793 4.2:-1400130 MS.gene62678:CDS
AAGGGTGGTGGCTGGTCCTC+AGG 0.380509 4.2:-1398022 MS.gene62678:CDS
GAGCCTGAGCCTGAGGAGCT+TGG 0.384449 4.2:+1397926 None:intergenic
AAAGGTGGTGGCTGCTCGTC+AGG 0.385388 4.2:-1398340 MS.gene62678:CDS
CTTCTTTGTCTTCTTTGTCC+GGG 0.397717 4.2:+1399895 None:intergenic
TCCCCAAGCTCCTCAGGCTC+AGG 0.401800 4.2:-1397929 MS.gene62678:CDS
GCAGGTAGCTAGTCATCTTC+AGG 0.404754 4.2:-1399457 MS.gene62678:intron
CTTGAATTTGATAGCACAAA+AGG 0.421977 4.2:-1400384 MS.gene62678:CDS
CTCAGCCTCAAGAGCTAGGA+AGG 0.422711 4.2:-1398275 MS.gene62678:CDS
CATGATCATGCAGTTGGTCT+TGG 0.425207 4.2:-1398645 MS.gene62678:CDS
GAGCTGGGAAGGGTGGTGGC+TGG 0.425258 4.2:-1398420 MS.gene62678:CDS
AAGGATGGCGGCTGGTCCTC+AGG 0.425842 4.2:-1398376 MS.gene62678:CDS
GCAAGTGGAGGTTGGTCCTC+AGG 0.426953 4.2:-1398448 MS.gene62678:CDS
ACAAGAACTTTAACATTGGC+TGG 0.429953 4.2:+1400603 None:intergenic
AAGGGTGGTGGCTTGTCCTC+AGG 0.433345 4.2:-1398136 MS.gene62678:CDS
CTCAGCCTCAAGAGCTAGGA+AGG 0.439395 4.2:-1397870 MS.gene62678:CDS
AAGGGTGGTGACTGGTCCTC+AGG 0.440118 4.2:-1398256 MS.gene62678:CDS
ATATGTGGCATCATGCTGTT+AGG 0.443627 4.2:-1400060 MS.gene62678:CDS
CTCAGGCTCAAGAGCTAGGA+AGG 0.446172 4.2:-1397912 MS.gene62678:CDS
AAGGGTGGTTGCTGCTCCAC+AGG 0.447691 4.2:-1398178 MS.gene62678:CDS
CTCAGACTCGAAAGCTAAGA+AGG 0.449485 4.2:-1398041 MS.gene62678:CDS
CAACAATATCCAATGGCATA+TGG 0.455357 4.2:-1398795 MS.gene62678:CDS
AATGAGCAATTGGGGATTGA+AGG 0.455531 4.2:-1400004 MS.gene62678:CDS
AAATAAACATCAACAAGAAC+AGG 0.463567 4.2:-1398919 MS.gene62678:CDS
GCCTGAGCCTGAGGAGCTTG+GGG 0.466889 4.2:+1397928 None:intergenic
AACACAAGATATACTAATTG+AGG 0.466931 4.2:-1397826 None:intergenic
TCAAGAGCTGGGAAGGGTGG+TGG 0.474375 4.2:-1398424 MS.gene62678:CDS
CTCAGGTTCAAGAGCTGGGA+AGG 0.474845 4.2:-1398431 MS.gene62678:CDS
CTCAACCTCAAAAGCTAGGA+AGG 0.475887 4.2:-1398155 MS.gene62678:CDS
CTCAACATCAAGAGCTAGGA+AGG 0.478408 4.2:-1398317 MS.gene62678:CDS
CTCAGGCTCAAGAGCTAGGA+AGG 0.478532 4.2:-1398005 MS.gene62678:CDS
CTCAGGCTCAAGAGCTAGGA+AGG 0.478532 4.2:-1398119 MS.gene62678:CDS
CTCAACCTCAAGAGCTAGGA+AGG 0.478532 4.2:-1398197 MS.gene62678:CDS
CTCAGGCTCAAGAGCTAGGA+AGG 0.478532 4.2:-1398239 MS.gene62678:CDS
TAAAGAATGCGTACTGGAAG+TGG 0.480432 4.2:+1399778 None:intergenic
AACAATTCTTGAACATATGA+AGG 0.483635 4.2:-1399499 MS.gene62678:CDS
TATATTTACTTACTAATGAC+AGG 0.483959 4.2:+1400272 None:intergenic
TGGTCCTCAGGTTCAAGAGC+TGG 0.485261 4.2:-1398436 MS.gene62678:CDS
CTTCCTAGCTCTTGATCTTG+AGG 0.489964 4.2:+1398396 None:intergenic
CCATGATGCAGGTTCGTGTT+GGG 0.494378 4.2:+1398717 None:intergenic
TGTTCTGAAGGATCAACAAA+TGG 0.496320 4.2:-1400154 MS.gene62678:CDS
TTTCCCCTTCCTAGCTCTTG+AGG 0.499640 4.2:+1397865 None:intergenic
AGCTCTTGAGGCTGAGCTTG+AGG 0.500477 4.2:+1398084 None:intergenic
AGCTCTTGAGGCTGAGCTTG+AGG 0.500477 4.2:+1398282 None:intergenic
AAGGGTGGTGGCTACTCCAC+AGG 0.505062 4.2:-1397893 MS.gene62678:CDS
TCCATGATGCAGGTTCGTGT+TGG 0.506466 4.2:+1398716 None:intergenic
ATCAACAAATGGAATAATGA+AGG 0.507137 4.2:-1400143 MS.gene62678:CDS
CGAAGAAGGAGCGGGTGGTA+TGG 0.509910 4.2:-1399853 MS.gene62678:CDS
TGAAGCTGAAATGCCGGACA+TGG 0.511513 4.2:-1400339 MS.gene62678:CDS
AAGAGCTAGGAAGGGGAAAG+AGG 0.511686 4.2:-1397861 MS.gene62678:CDS
GAGCTAGGAATGGTGGTGGC+TGG 0.512918 4.2:-1398066 MS.gene62678:CDS
TTCATCTGAGGAACATGCAT+TGG 0.513254 4.2:+1398555 None:intergenic
CTCAAGATCAAGAGCTAGGA+AGG 0.513303 4.2:-1398395 MS.gene62678:CDS
GGAGCAAGCTCTCATGGTTG+TGG 0.514199 4.2:-1398898 MS.gene62678:CDS
GAAGAAGACGAAGAAGGAGC+GGG 0.515637 4.2:-1399861 MS.gene62678:CDS
GATTGAAGGTAGCCAAATAA+GGG 0.517817 4.2:-1399990 MS.gene62678:CDS
GCCTAGACTTACAAGGGAGC+AGG 0.518181 4.2:-1399475 MS.gene62678:CDS
CAAGCTCAGCCTCAAGAGCT+AGG 0.520411 4.2:-1398279 MS.gene62678:CDS
GGTCCTCAGGCTCAAGAGCT+AGG 0.520740 4.2:-1398009 MS.gene62678:CDS
TGTCCTCAGGCTCAAGAGCT+AGG 0.520740 4.2:-1398123 MS.gene62678:CDS
GGTCCTCAGGCTCAAGAGCT+AGG 0.520740 4.2:-1398243 MS.gene62678:CDS
GGTCCTCAGGCTCAAGAGCT+AGG 0.520740 4.2:-1398363 MS.gene62678:CDS
AGCTCTTGAGGCTGAGCCTG+TGG 0.521609 4.2:+1397877 None:intergenic
AGCTCTTGAGGTTGAGCATG+TGG 0.521665 4.2:+1398204 None:intergenic
CAGGCTCAGGCTCAAGAGCT+AGG 0.522151 4.2:-1397916 MS.gene62678:CDS
CAAGCTCAGCCTCAAGAGCT+AGG 0.524513 4.2:-1398081 MS.gene62678:CDS
AAAATCAAGTAAAGTATATG+TGG 0.524588 4.2:-1400075 MS.gene62678:CDS
AGTTGGTCTTGGAAACTTGA+TGG 0.528215 4.2:-1398634 MS.gene62678:CDS
AATCACAAGAACTTTAACAT+TGG 0.529369 4.2:+1400599 None:intergenic
CAGGCTCAGCCTCAAGAGCT+AGG 0.529994 4.2:-1397874 MS.gene62678:CDS
AGCAGCAGCATGTGAACTGT+TGG 0.530394 4.2:+1398872 None:intergenic
AGAACAGGAGCAAGCTCTCA+TGG 0.536290 4.2:-1398904 MS.gene62678:CDS
GAGCTAGGAAGGGTGGTGAC+TGG 0.538059 4.2:-1398264 MS.gene62678:CDS
TCCACGATGTTGTCCACACT+CGG 0.540582 4.2:+1398510 None:intergenic
GAGCTAGGAAGGGTGGTGGC+TGG 0.541935 4.2:-1398108 MS.gene62678:CDS
GAGCTAGGAAGGGTGGTGGC+TGG 0.541935 4.2:-1398306 MS.gene62678:CDS
ACACAAGATATACTAATTGA+GGG 0.543203 4.2:-1397825 None:intergenic
GGTCCTCAGGTTCAAGAGCT+GGG 0.544277 4.2:-1398435 MS.gene62678:CDS
TTCTTTGTCTTCTTTGTCCG+GGG 0.544906 4.2:+1399896 None:intergenic
AAATCAAATGAATCCATGTC+CGG 0.545425 4.2:+1400326 None:intergenic
GAAGACGAAGAAGACGAAGA+AGG 0.546374 4.2:-1399867 MS.gene62678:CDS
AGCTCTTGAGCCTGAGCCTG+AGG 0.546926 4.2:+1397919 None:intergenic
ACATGCATTGGTGATTGTTG+TGG 0.548414 4.2:+1398567 None:intergenic
ACTGATCATGATCATGCAGT+TGG 0.548759 4.2:-1398651 MS.gene62678:CDS
GGTTCAAGAGCTGGGAAGGG+TGG 0.548919 4.2:-1398427 MS.gene62678:CDS
CCCAACACGAACCTGCATCA+TGG 0.549931 4.2:-1398717 MS.gene62678:CDS
AAGCTAAGAAGGGTGGTGGC+TGG 0.555418 4.2:-1398030 MS.gene62678:CDS
ATGTTGCATATCCATGATGC+AGG 0.557330 4.2:+1398706 None:intergenic
CAGGCTCAACATCAAGAGCT+AGG 0.558850 4.2:-1398321 MS.gene62678:CDS
CAGGCTCAACCTCAAAAGCT+AGG 0.561577 4.2:-1398159 MS.gene62678:CDS
TTTGTCTTCTTTGTCCGGGG+TGG 0.562381 4.2:+1399899 None:intergenic
CATGCTCAACCTCAAGAGCT+AGG 0.564867 4.2:-1398201 MS.gene62678:CDS
AAGCTAGGAAGGGTGGTGGC+TGG 0.568049 4.2:-1397952 MS.gene62678:CDS
GGCTGATGAGGAAAATGCCT+TGG 0.570075 4.2:+1398738 None:intergenic
TTTCCTAGCTCTTGAGCCTG+AGG 0.573303 4.2:+1398360 None:intergenic
GAGCTAGGAAGGATGGCGGC+TGG 0.575246 4.2:-1398384 MS.gene62678:CDS
TGAAGGTGCCTAGACTTACA+AGG 0.576956 4.2:-1399482 MS.gene62678:CDS
ACCTGCTCCCTTGTAAGTCT+AGG 0.577890 4.2:+1399474 None:intergenic
GGCTCAAGAGCTAGGAAGGG+TGG 0.577942 4.2:-1398001 MS.gene62678:CDS
TGCATCATGGATATGCAACA+TGG 0.578384 4.2:-1398704 MS.gene62678:CDS
ACCACCCTTCCTAGCTCTTG+AGG 0.578654 4.2:+1398192 None:intergenic
GGCTCAAGAGCTAGGAAAGG+TGG 0.579566 4.2:-1398355 MS.gene62678:CDS
GCCTCAAGAGCTAGGAATGG+TGG 0.580235 4.2:-1398073 MS.gene62678:CDS
CAGCCTCAAGAGCTAGGAAG+GGG 0.581740 4.2:-1397868 MS.gene62678:CDS
GGTCCTCAAGATCAAGAGCT+AGG 0.581757 4.2:-1398399 MS.gene62678:CDS
TTCCCAGAAAATTGATACCA+AGG 0.582328 4.2:-1398755 MS.gene62678:CDS
ACCACCCTTCCTAGCTCTTG+AGG 0.582587 4.2:+1398270 None:intergenic
GGCTCAAGAGCTAGGAAGGG+TGG 0.582674 4.2:-1397908 MS.gene62678:CDS
GGCTCAAGAGCTAGGAAGGG+TGG 0.582674 4.2:-1398115 MS.gene62678:CDS
GGCTCAAGAGCTAGGAAGGG+TGG 0.582674 4.2:-1398235 MS.gene62678:CDS
GCCTCAAGAGCTAGGAAGGG+TGG 0.582674 4.2:-1398271 MS.gene62678:CDS
CTTCCTAGCTCTTGAGCCTG+AGG 0.585618 4.2:+1398006 None:intergenic
CTTCCTAGCTCTTGAGCCTG+AGG 0.585618 4.2:+1398240 None:intergenic
ACTACTTGAAGCTGAAATGC+CGG 0.587583 4.2:-1400345 MS.gene62678:CDS
TCAAAAGCTAGGAAGGGTGG+TGG 0.595324 4.2:-1398148 MS.gene62678:CDS
AGATCAAGAGCTAGGAAGGA+TGG 0.596478 4.2:-1398391 MS.gene62678:CDS
TTATGATGTTCTTGTTGCTG+AGG 0.596660 4.2:+1398591 None:intergenic
TGGTCTCAATTCAATTACCA+AGG 0.596923 4.2:-1400537 MS.gene62678:intron
ACGCTTAAACGATGTACCAT+CGG 0.602369 4.2:-1399948 MS.gene62678:CDS
GAACTGCAACCATATGCCAT+TGG 0.602482 4.2:+1398786 None:intergenic
GCTACAATGCAAGTGGAGGT+TGG 0.607441 4.2:-1398456 MS.gene62678:CDS
CGAAGAAGACGAAGAAGGAG+CGG 0.608903 4.2:-1399862 MS.gene62678:CDS
TCGAAAGCTAAGAAGGGTGG+TGG 0.609238 4.2:-1398034 MS.gene62678:CDS
ACCTCAAAAGCTAGGAAGGG+TGG 0.610885 4.2:-1398151 MS.gene62678:CDS
CGGTGAAGCTACAATGCAAG+TGG 0.610968 4.2:-1398463 MS.gene62678:CDS
CTTCCCAGCTCTTGAACCTG+AGG 0.611183 4.2:+1398432 None:intergenic
TTGACTGCATTATTCATCTG+AGG 0.611554 4.2:+1398543 None:intergenic
TCAAGAGCTAGGAAGGGTGG+TGG 0.612518 4.2:-1398310 MS.gene62678:CDS
TCAGTTATATAATATTCACT+TGG 0.614757 4.2:+1398669 None:intergenic
GACTCGAAAGCTAAGAAGGG+TGG 0.616097 4.2:-1398037 MS.gene62678:CDS
ACCACCATTCCTAGCTCTTG+AGG 0.617365 4.2:+1398072 None:intergenic
ACCTCAAGAGCTAGGAAGGG+TGG 0.617706 4.2:-1398193 MS.gene62678:CDS
TCAGCCTCAAGAGCTAGGAA+GGG 0.618998 4.2:-1397869 MS.gene62678:CDS
CTTCCTAGCTCTTGAGCCTG+AGG 0.620806 4.2:+1398120 None:intergenic
TCAGGCTCAAGAGCTAGGAA+GGG 0.624572 4.2:-1397911 MS.gene62678:CDS
AAGATATACCCGAACCACCC+CGG 0.626205 4.2:-1399913 MS.gene62678:CDS
TGCAGAACAACAATATCCAA+TGG 0.629974 4.2:-1398802 MS.gene62678:CDS
GGCTGGAAACTCATTCCCAA+AGG 0.633277 4.2:+1400620 None:intergenic
CAATGATGTTGTACCGAGTG+TGG 0.635078 4.2:-1398523 MS.gene62678:CDS
TCAGGTTCAAGAGCTGGGAA+GGG 0.636064 4.2:-1398430 MS.gene62678:CDS
TCAAGAGCTAGGAAGGGTGG+TGG 0.636286 4.2:-1398112 MS.gene62678:CDS
TCAAGAGCTAGGAAGGGTGG+TGG 0.636286 4.2:-1398232 MS.gene62678:CDS
TCAAGAGCTAGGAATGGTGG+TGG 0.637451 4.2:-1398070 MS.gene62678:CDS
TCAAGAGCTAGGAAAGGTGG+TGG 0.637451 4.2:-1398352 MS.gene62678:CDS
TCAACCTCAAAAGCTAGGAA+GGG 0.637506 4.2:-1398154 MS.gene62678:CDS
TCAAGAGCTAGGAAGGGTGG+TGG 0.638725 4.2:-1397905 MS.gene62678:CDS
TCAGACTCGAAAGCTAAGAA+GGG 0.638931 4.2:-1398040 MS.gene62678:CDS
TCAGCCTCAAGAGCTAGGAA+TGG 0.643593 4.2:-1398076 MS.gene62678:CDS
TCAATGCTATTGAGAAAACA+TGG 0.646799 4.2:-1400557 MS.gene62678:CDS
ACATCAAGAGCTAGGAAGGG+TGG 0.650678 4.2:-1398313 MS.gene62678:CDS
TCAGCCTCAAGAGCTAGGAA+GGG 0.652611 4.2:-1398274 MS.gene62678:CDS
AGGTTCGTGTTGGGCTGATG+AGG 0.653657 4.2:+1398726 None:intergenic
TCAGGCTCAAGAGCTAGGAA+AGG 0.654595 4.2:-1398358 MS.gene62678:CDS
GAAGACGAAGAAGGAGCGGG+TGG 0.656592 4.2:-1399858 MS.gene62678:CDS
GCATCATGGATATGCAACAT+GGG 0.656904 4.2:-1398703 MS.gene62678:CDS
GAAGGTGCCTAGACTTACAA+GGG 0.661563 4.2:-1399481 MS.gene62678:CDS
GCTGTTAGGAAAATGATGAG+AGG 0.661716 4.2:-1400046 MS.gene62678:CDS
TGAAGCTACAATGCAAGTGG+AGG 0.663678 4.2:-1398460 MS.gene62678:CDS
ACCGAGTGTGGACAACATCG+TGG 0.681310 4.2:-1398511 MS.gene62678:CDS
TCAGGCTCAAGAGCTAGGAA+GGG 0.685952 4.2:-1398004 MS.gene62678:CDS
TCAGGCTCAAGAGCTAGGAA+GGG 0.685952 4.2:-1398118 MS.gene62678:CDS
TCAGGCTCAAGAGCTAGGAA+GGG 0.685952 4.2:-1398238 MS.gene62678:CDS
GCAGTGACGATGTGTTCTGA+AGG 0.690924 4.2:-1400166 MS.gene62678:intron
TCAACATCAAGAGCTAGGAA+GGG 0.691703 4.2:-1398316 MS.gene62678:CDS
TCAACCTCAAGAGCTAGGAA+GGG 0.693140 4.2:-1398196 MS.gene62678:CDS
TCAAGAGCTAGGAAGGATGG+CGG 0.700470 4.2:-1398388 MS.gene62678:CDS
CTGTATTGAAATCATCACAG+TGG 0.740386 4.2:-1398842 MS.gene62678:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTAAAAATTTGATTATTTA+TGG - chr4.2:1399109-1399128 MS.gene62678:intron 10.0%
!!! ACATATTATTAATCTTTTAT+AGG - chr4.2:1399177-1399196 MS.gene62678:intron 10.0%
!! AAAGTTTAAATTTCAAAGAA+CGG + chr4.2:1399043-1399062 None:intergenic 15.0%
!! AAATTTGATTATTTATGGTT+TGG - chr4.2:1399114-1399133 MS.gene62678:intron 15.0%
!! AAGGTTTGTAAATTAATATA+TGG - chr4.2:1397940-1397959 MS.gene62678:CDS 15.0%
!! ATATATTAATTTACAAACCT+TGG + chr4.2:1397941-1397960 None:intergenic 15.0%
!! TGTCAAAAATGAATAAATAA+AGG - chr4.2:1399072-1399091 MS.gene62678:intron 15.0%
!! AAAATCAAGTAAAGTATATG+TGG - chr4.2:1398383-1398402 MS.gene62678:CDS 20.0%
!! AATAATACTGTAACAGATTT+CGG - chr4.2:1399975-1399994 MS.gene62678:CDS 20.0%
!! ATTCAATTAACAATCAAAGA+AGG + chr4.2:1397968-1397987 None:intergenic 20.0%
!! TATATTTACTTACTAATGAC+AGG + chr4.2:1398189-1398208 None:intergenic 20.0%
!! TATTGTCTTGGTTTAATTAA+TGG + chr4.2:1399417-1399436 None:intergenic 20.0%
!! TCAGTTATATAATATTCACT+TGG + chr4.2:1399792-1399811 None:intergenic 20.0%
!!! AGGAATCTCAATATATTTTA+TGG - chr4.2:1399197-1399216 MS.gene62678:intron 20.0%
!!! ATATGTTCAAGAATTGTTTT+TGG + chr4.2:1398957-1398976 None:intergenic 20.0%
!!! ATTAGTCTTTAACTTTCATT+CGG - chr4.2:1399223-1399242 MS.gene62678:intron 20.0%
!!! CTTTTGATAAAAACAAGAAT+AGG + chr4.2:1398706-1398725 None:intergenic 20.0%
!!! GATAGTGTAAAATTTGTTTT+TGG - chr4.2:1398839-1398858 MS.gene62678:CDS 20.0%
! AAAAAATCAATGAGCAATTG+GGG - chr4.2:1398446-1398465 MS.gene62678:CDS 25.0%
! AAAAAGATTGAGACTTTCAT+AGG + chr4.2:1397999-1398018 None:intergenic 25.0%
! AAATAAACATCAACAAGAAC+AGG - chr4.2:1399539-1399558 MS.gene62678:intron 25.0%
! AACAATTCTTGAACATATGA+AGG - chr4.2:1398959-1398978 MS.gene62678:intron 25.0%
! AATCACAAGAACTTTAACAT+TGG + chr4.2:1397862-1397881 None:intergenic 25.0%
! ACAAAAAATCAATGAGCAAT+TGG - chr4.2:1398444-1398463 MS.gene62678:CDS 25.0%
! ATAATGAAGGCAATTGAAAA+TGG - chr4.2:1398328-1398347 MS.gene62678:CDS 25.0%
! ATCAACAAATGGAATAATGA+AGG - chr4.2:1398315-1398334 MS.gene62678:CDS 25.0%
! CAAAAAATCAATGAGCAATT+GGG - chr4.2:1398445-1398464 MS.gene62678:CDS 25.0%
! CAATTAGTATATCTTGTGTT+AGG + chr4.2:1400632-1400651 None:intergenic 25.0%
! TAGTGTACAATTTATTGTCT+TGG + chr4.2:1399429-1399448 None:intergenic 25.0%
! TCTTGGTTTAATTAATGGTA+TGG + chr4.2:1399412-1399431 None:intergenic 25.0%
! TTAGATGTTAGAGTAAACTA+CGG + chr4.2:1398743-1398762 None:intergenic 25.0%
!! TTATGGTTTGGAAAATTTGA+CGG - chr4.2:1399126-1399145 MS.gene62678:intron 25.0%
!! TTTACTTGATTTTCAGTGAA+TGG + chr4.2:1398376-1398395 None:intergenic 25.0%
AAATCAAATGAATCCATGTC+CGG + chr4.2:1398135-1398154 None:intergenic 30.0%
ATCATTAAAGTGACATGTAG+CGG - chr4.2:1398790-1398809 MS.gene62678:CDS 30.0%
TCAATGCTATTGAGAAAACA+TGG - chr4.2:1397901-1397920 MS.gene62678:CDS 30.0%
TTCTGCTCGATTAAGTATTA+TGG + chr4.2:1399347-1399366 None:intergenic 30.0%
! CTTGAATTTGATAGCACAAA+AGG - chr4.2:1398074-1398093 MS.gene62678:CDS 30.0%
! CTTTTGTGCTATCAAATTCA+AGG + chr4.2:1398076-1398095 None:intergenic 30.0%
! TATGGTTTGGAAAATTTGAC+GGG - chr4.2:1399127-1399146 MS.gene62678:intron 30.0%
!!! ACAAAAGGTGCATTTTGATT+TGG - chr4.2:1398089-1398108 MS.gene62678:CDS 30.0%
!!! CTTTGTTTGTTTTTTTCCAG+AGG - chr4.2:1398926-1398945 MS.gene62678:CDS 30.0%
AATAGGTAAAGAATGCGTAC+TGG + chr4.2:1398689-1398708 None:intergenic 35.0%
AATCATCTCTTGCCCTTATT+TGG + chr4.2:1398483-1398502 None:intergenic 35.0%
ACAAGAACTTTAACATTGGC+TGG + chr4.2:1397858-1397877 None:intergenic 35.0%
ATTACCAGTTTACCCTGAAA+AGG - chr4.2:1399252-1399271 MS.gene62678:intron 35.0%
CAACAATATCCAATGGCATA+TGG - chr4.2:1399663-1399682 MS.gene62678:intron 35.0%
CTGTATTGAAATCATCACAG+TGG - chr4.2:1399616-1399635 MS.gene62678:intron 35.0%
GATTGAAGGTAGCCAAATAA+GGG - chr4.2:1398468-1398487 MS.gene62678:CDS 35.0%
TCAACCTAAAAAGCTAGGAA+GGG - chr4.2:1400496-1400515 MS.gene62678:intron 35.0%
TCTTCTTTGTCTTCTTTGTC+CGG + chr4.2:1398567-1398586 None:intergenic 35.0%
TGCAGAACAACAATATCCAA+TGG - chr4.2:1399656-1399675 MS.gene62678:intron 35.0%
TGGTCTCAATTCAATTACCA+AGG - chr4.2:1397921-1397940 MS.gene62678:CDS 35.0%
TTATGATGTTCTTGTTGCTG+AGG + chr4.2:1399870-1399889 None:intergenic 35.0%
TTCCCAGAAAATTGATACCA+AGG - chr4.2:1399703-1399722 MS.gene62678:intron 35.0%
TTGACTGCATTATTCATCTG+AGG + chr4.2:1399918-1399937 None:intergenic 35.0%
! ATGCCTTGGTATCAATTTTC+TGG + chr4.2:1399709-1399728 None:intergenic 35.0%
! TGCCTTGGTATCAATTTTCT+GGG + chr4.2:1399708-1399727 None:intergenic 35.0%
!! TGTTCTGAAGGATCAACAAA+TGG - chr4.2:1398304-1398323 MS.gene62678:CDS 35.0%
!! TTTGAGTTAGCTCCTTTTCA+GGG + chr4.2:1399267-1399286 None:intergenic 35.0%
!!! TGTTTGTTTTTTTCCAGAGG+CGG - chr4.2:1398929-1398948 MS.gene62678:CDS 35.0%
!!! TTTTGAGTTAGCTCCTTTTC+AGG + chr4.2:1399268-1399287 None:intergenic 35.0%
AACGATGTACCATCGGAAAA+AGG - chr4.2:1398517-1398536 MS.gene62678:CDS 40.0%
AATGAGCAATTGGGGATTGA+AGG - chr4.2:1398454-1398473 MS.gene62678:CDS 40.0%
ACGCTTAAACGATGTACCAT+CGG - chr4.2:1398510-1398529 MS.gene62678:CDS 40.0%
ACTACTTGAAGCTGAAATGC+CGG - chr4.2:1398113-1398132 MS.gene62678:CDS 40.0%
ACTGATCATGATCATGCAGT+TGG - chr4.2:1399807-1399826 MS.gene62678:CDS 40.0%
ATATGTGGCATCATGCTGTT+AGG - chr4.2:1398398-1398417 MS.gene62678:CDS 40.0%
ATGTTGCATATCCATGATGC+AGG + chr4.2:1399755-1399774 None:intergenic 40.0%
CATGCTCAACCTAAAAAGCT+AGG - chr4.2:1400491-1400510 MS.gene62678:intron 40.0%
CTCAACCTAAAAAGCTAGGA+AGG - chr4.2:1400495-1400514 MS.gene62678:intron 40.0%
CTTCTTTGTCTTCTTTGTCC+GGG + chr4.2:1398566-1398585 None:intergenic 40.0%
GCATCATGGATATGCAACAT+GGG - chr4.2:1399755-1399774 MS.gene62678:intron 40.0%
GCTGTTAGGAAAATGATGAG+AGG - chr4.2:1398412-1398431 MS.gene62678:CDS 40.0%
GGATTGAAGGTAGCCAAATA+AGG - chr4.2:1398467-1398486 MS.gene62678:CDS 40.0%
TAAAGAATGCGTACTGGAAG+TGG + chr4.2:1398683-1398702 None:intergenic 40.0%
TCAACATCAAGAGCTAGGAA+GGG - chr4.2:1400142-1400161 MS.gene62678:CDS 40.0%
TCAACCTCAAAAGCTAGGAA+GGG - chr4.2:1400304-1400323 MS.gene62678:CDS 40.0%
TCAGACTCGAAAGCTAAGAA+GGG - chr4.2:1400418-1400437 MS.gene62678:intron 40.0%
TGCATCATGGATATGCAACA+TGG - chr4.2:1399754-1399773 MS.gene62678:intron 40.0%
TTCATCTGAGGAACATGCAT+TGG + chr4.2:1399906-1399925 None:intergenic 40.0%
TTCTTTGTCTTCTTTGTCCG+GGG + chr4.2:1398565-1398584 None:intergenic 40.0%
! ACATGCATTGGTGATTGTTG+TGG + chr4.2:1399894-1399913 None:intergenic 40.0%
!! AGTTGGTCTTGGAAACTTGA+TGG - chr4.2:1399824-1399843 MS.gene62678:CDS 40.0%
!!! AGCTTTTTAGGTTGAGCATG+TGG + chr4.2:1400491-1400510 None:intergenic 40.0%
ACCTAAAAAGCTAGGAAGGG+TGG - chr4.2:1400499-1400518 MS.gene62678:intron 45.0%
AGATCAAGAGCTAGGAAGGA+TGG - chr4.2:1400067-1400086 MS.gene62678:CDS 45.0%
CAATGATGTTGTACCGAGTG+TGG - chr4.2:1399935-1399954 MS.gene62678:CDS 45.0%
CTCAACATCAAGAGCTAGGA+AGG - chr4.2:1400141-1400160 MS.gene62678:CDS 45.0%
CTCAACCTCAAAAGCTAGGA+AGG - chr4.2:1400303-1400322 MS.gene62678:CDS 45.0%
CTCAAGATCAAGAGCTAGGA+AGG - chr4.2:1400063-1400082 MS.gene62678:CDS 45.0%
CTCAGACTCGAAAGCTAAGA+AGG - chr4.2:1400417-1400436 MS.gene62678:intron 45.0%
GAACTGCAACCATATGCCAT+TGG + chr4.2:1399675-1399694 None:intergenic 45.0%
GAAGACGAAGAAGACGAAGA+AGG - chr4.2:1398591-1398610 MS.gene62678:CDS 45.0%
TAAAAAGCTAGGAAGGGTGG+TGG - chr4.2:1400502-1400521 MS.gene62678:intron 45.0%
TCAACCTCAAGAGCTAGGAA+GGG - chr4.2:1400262-1400281 MS.gene62678:intron 45.0%
TGAAGCTACAATGCAAGTGG+AGG - chr4.2:1399998-1400017 MS.gene62678:CDS 45.0%
! CATGATCATGCAGTTGGTCT+TGG - chr4.2:1399813-1399832 MS.gene62678:CDS 45.0%
! CTTCCTAGCTCTTGATCTTG+AGG + chr4.2:1400065-1400084 None:intergenic 45.0%
! TCTTCGTCACCTTTTTCCGA+TGG + chr4.2:1398529-1398548 None:intergenic 45.0%
!! ACCACCCTTCCTAGCTTTTT+AGG + chr4.2:1400503-1400522 None:intergenic 45.0%
!! AGCTCCTTTTCAGGGTAAAC+TGG + chr4.2:1399259-1399278 None:intergenic 45.0%
!! GAAGGTGCCTAGACTTACAA+GGG - chr4.2:1398977-1398996 MS.gene62678:intron 45.0%
!! TGAAGGTGCCTAGACTTACA+AGG - chr4.2:1398976-1398995 MS.gene62678:intron 45.0%
!!! ATTGTTTTTGGTACCGCCTC+TGG + chr4.2:1398945-1398964 None:intergenic 45.0%
AAGAGCTAGGAAGGGGAAAG+AGG - chr4.2:1400597-1400616 MS.gene62678:CDS 50.0%
AAGATATACCCGAACCACCC+CGG - chr4.2:1398545-1398564 MS.gene62678:CDS 50.0%
ACATCAAGAGCTAGGAAGGG+TGG - chr4.2:1400145-1400164 MS.gene62678:CDS 50.0%
ACCTCAAAAGCTAGGAAGGG+TGG - chr4.2:1400307-1400326 MS.gene62678:CDS 50.0%
ACCTGCTCCCTTGTAAGTCT+AGG + chr4.2:1398987-1399006 None:intergenic 50.0%
AGAACAGGAGCAAGCTCTCA+TGG - chr4.2:1399554-1399573 MS.gene62678:intron 50.0%
AGCAGCAGCATGTGAACTGT+TGG + chr4.2:1399589-1399608 None:intergenic 50.0%
AGCTCTTGAGGTTGAGCATG+TGG + chr4.2:1400257-1400276 None:intergenic 50.0%
CAGGCTCAACATCAAGAGCT+AGG - chr4.2:1400137-1400156 MS.gene62678:CDS 50.0%
CAGGCTCAACCTCAAAAGCT+AGG - chr4.2:1400299-1400318 MS.gene62678:CDS 50.0%
CATGCTCAACCTCAAGAGCT+AGG - chr4.2:1400257-1400276 MS.gene62678:intron 50.0%
CCATGATGCAGGTTCGTGTT+GGG + chr4.2:1399744-1399763 None:intergenic 50.0%
CGAAGAAGACGAAGAAGGAG+CGG - chr4.2:1398596-1398615 MS.gene62678:CDS 50.0%
CGGTGAAGCTACAATGCAAG+TGG - chr4.2:1399995-1400014 MS.gene62678:CDS 50.0%
CTCAACCTCAAGAGCTAGGA+AGG - chr4.2:1400261-1400280 MS.gene62678:intron 50.0%
GAAGAAGACGAAGAAGGAGC+GGG - chr4.2:1398597-1398616 MS.gene62678:CDS 50.0%
GACTCGAAAGCTAAGAAGGG+TGG - chr4.2:1400421-1400440 MS.gene62678:intron 50.0%
GCAGGTAGCTAGTCATCTTC+AGG - chr4.2:1399001-1399020 MS.gene62678:intron 50.0%
GCAGTGACGATGTGTTCTGA+AGG - chr4.2:1398292-1398311 MS.gene62678:CDS 50.0%
GCTACAATGCAAGTGGAGGT+TGG - chr4.2:1400002-1400021 MS.gene62678:CDS 50.0%
GGCTGATGAGGAAAATGCCT+TGG + chr4.2:1399723-1399742 None:intergenic 50.0%
GGCTGGAAACTCATTCCCAA+AGG + chr4.2:1397841-1397860 None:intergenic 50.0%
GGTCCTCAAGATCAAGAGCT+AGG - chr4.2:1400059-1400078 MS.gene62678:CDS 50.0%
TCAAAAGCTAGGAAGGGTGG+TGG - chr4.2:1400310-1400329 MS.gene62678:CDS 50.0%
TCAAGAGCTAGGAAAGGTGG+TGG - chr4.2:1400106-1400125 MS.gene62678:CDS 50.0%
TCAAGAGCTAGGAAGGATGG+CGG - chr4.2:1400070-1400089 MS.gene62678:CDS 50.0%
TCAAGAGCTAGGAATGGTGG+TGG - chr4.2:1400388-1400407 MS.gene62678:CDS 50.0%
TCAGCCTCAAGAGCTAGGAA+GGG - chr4.2:1400184-1400203 MS.gene62678:intron 50.0%
TCAGCCTCAAGAGCTAGGAA+TGG - chr4.2:1400184-1400203 MS.gene62678:intron 50.0%
TCAGGCTCAAGAGCTAGGAA+AGG - chr4.2:1400100-1400119 MS.gene62678:CDS 50.0%
TCAGGCTCAAGAGCTAGGAA+GGG - chr4.2:1400100-1400119 MS.gene62678:CDS 50.0%
TCAGGTTCAAGAGCTGGGAA+GGG - chr4.2:1400028-1400047 MS.gene62678:CDS 50.0%
TCCACGATGTTGTCCACACT+CGG + chr4.2:1399951-1399970 None:intergenic 50.0%
TCCATGATGCAGGTTCGTGT+TGG + chr4.2:1399745-1399764 None:intergenic 50.0%
TCGAAAGCTAAGAAGGGTGG+TGG - chr4.2:1400424-1400443 MS.gene62678:intron 50.0%
TGAAGCTGAAATGCCGGACA+TGG - chr4.2:1398119-1398138 MS.gene62678:CDS 50.0%
TTTGTCTTCTTTGTCCGGGG+TGG + chr4.2:1398562-1398581 None:intergenic 50.0%
! ACCACCATTCCTAGCTCTTG+AGG + chr4.2:1400389-1400408 None:intergenic 50.0%
! TTTCCCCTTCCTAGCTCTTG+AGG + chr4.2:1400596-1400615 None:intergenic 50.0%
! TTTCCTAGCTCTTGAGCCTG+AGG + chr4.2:1400101-1400120 None:intergenic 50.0%
!! ACCACCCTTCCTAGCTTTTG+AGG + chr4.2:1400311-1400330 None:intergenic 50.0%
!!! AGCTTTTGAGGTTGAGCCTG+TGG + chr4.2:1400299-1400318 None:intergenic 50.0%
ACCGAGTGTGGACAACATCG+TGG - chr4.2:1399947-1399966 MS.gene62678:CDS 55.0%
ACCTCAAGAGCTAGGAAGGG+TGG - chr4.2:1400265-1400284 MS.gene62678:intron 55.0%
AGCTCTTGAGGCTGAGCTTG+AGG + chr4.2:1400179-1400198 None:intergenic 55.0%
AGGTTCGTGTTGGGCTGATG+AGG + chr4.2:1399735-1399754 None:intergenic 55.0%
CAAGCTCAGCCTCAAGAGCT+AGG - chr4.2:1400179-1400198 MS.gene62678:intron 55.0%
CAGCCTCAAGAGCTAGGAAG+GGG - chr4.2:1400185-1400204 MS.gene62678:intron 55.0%
CCCAACACGAACCTGCATCA+TGG - chr4.2:1399741-1399760 MS.gene62678:intron 55.0%
CTCAGCCTCAAGAGCTAGGA+AGG - chr4.2:1400183-1400202 MS.gene62678:intron 55.0%
CTCAGGCTCAAGAGCTAGGA+AGG - chr4.2:1400099-1400118 MS.gene62678:CDS 55.0%
CTCAGGTTCAAGAGCTGGGA+AGG - chr4.2:1400027-1400046 MS.gene62678:CDS 55.0%
CTTCCCAGCTCTTGAACCTG+AGG + chr4.2:1400029-1400048 None:intergenic 55.0%
CTTCTTTGTCCGGGGTGGTT+CGG + chr4.2:1398557-1398576 None:intergenic 55.0%
GCCTAGACTTACAAGGGAGC+AGG - chr4.2:1398983-1399002 MS.gene62678:intron 55.0%
GCCTCAAGAGCTAGGAATGG+TGG - chr4.2:1400385-1400404 MS.gene62678:CDS 55.0%
GGAGCAAGCTCTCATGGTTG+TGG - chr4.2:1399560-1399579 MS.gene62678:intron 55.0%
GGCTCAAGAGCTAGGAAAGG+TGG - chr4.2:1400103-1400122 MS.gene62678:CDS 55.0%
GGTCCTCAGGTTCAAGAGCT+GGG - chr4.2:1400023-1400042 MS.gene62678:CDS 55.0%
TCAAGAGCTAGGAAGGGTGG+TGG - chr4.2:1400148-1400167 MS.gene62678:CDS 55.0%
TGGTCCTCAGGTTCAAGAGC+TGG - chr4.2:1400022-1400041 MS.gene62678:CDS 55.0%
TGTCCTCAGGCTCAAGAGCT+AGG - chr4.2:1400335-1400354 MS.gene62678:CDS 55.0%
TTCTTTGTCCGGGGTGGTTC+GGG + chr4.2:1398556-1398575 None:intergenic 55.0%
! AAGCTAAGAAGGGTGGTGGC+TGG - chr4.2:1400428-1400447 MS.gene62678:intron 55.0%
! ACCACCCTTCCTAGCTCTTG+AGG + chr4.2:1400149-1400168 None:intergenic 55.0%
! CTTCCTAGCTCTTGAGCCTG+AGG + chr4.2:1400221-1400240 None:intergenic 55.0%
AAGGGTGGTTGCTGCTCCAC+AGG - chr4.2:1400280-1400299 MS.gene62678:intron 60.0%
AGCCTGAGCCTGAGGAGCTT+GGG + chr4.2:1400534-1400553 None:intergenic 60.0%
AGCTCTTGAGCCTGAGCCTG+AGG + chr4.2:1400542-1400561 None:intergenic 60.0%
AGCTCTTGAGGCTGAGCCTG+TGG + chr4.2:1400584-1400603 None:intergenic 60.0%
CAGGCTCAGCCTCAAGAGCT+AGG - chr4.2:1400584-1400603 MS.gene62678:CDS 60.0%
CAGGCTCAGGCTCAAGAGCT+AGG - chr4.2:1400542-1400561 MS.gene62678:CDS 60.0%
CGAAGAAGGAGCGGGTGGTA+TGG - chr4.2:1398605-1398624 MS.gene62678:CDS 60.0%
GAAGACGAAGAAGGAGCGGG+TGG - chr4.2:1398600-1398619 MS.gene62678:CDS 60.0%
GAGCTAGGAAGGGTGGTGAC+TGG - chr4.2:1400194-1400213 MS.gene62678:intron 60.0%
GCCTCAAGAGCTAGGAAGGG+TGG - chr4.2:1400187-1400206 MS.gene62678:intron 60.0%
GGCTCAAGAGCTAGGAAGGG+TGG - chr4.2:1400223-1400242 MS.gene62678:intron 60.0%
GGTCCTCAGGCTCAAGAGCT+AGG - chr4.2:1400095-1400114 MS.gene62678:CDS 60.0%
GGTTCAAGAGCTGGGAAGGG+TGG - chr4.2:1400031-1400050 MS.gene62678:CDS 60.0%
TCAAGAGCTGGGAAGGGTGG+TGG - chr4.2:1400034-1400053 MS.gene62678:CDS 60.0%
! AAAGGTGGTGGCTGCTCGTC+AGG - chr4.2:1400118-1400137 MS.gene62678:CDS 60.0%
! AAGCTAGGAAGGGTGGTGGC+TGG - chr4.2:1400314-1400333 MS.gene62678:CDS 60.0%
! AAGGGTGGTGACTGGTCCTC+AGG - chr4.2:1400202-1400221 MS.gene62678:intron 60.0%
! AAGGGTGGTGGCTACTCCAC+AGG - chr4.2:1400565-1400584 MS.gene62678:CDS 60.0%
! AAGGGTGGTGGCTTGTCCTC+AGG - chr4.2:1400322-1400341 MS.gene62678:CDS 60.0%
! GCAAGTGGAGGTTGGTCCTC+AGG - chr4.2:1400010-1400029 MS.gene62678:CDS 60.0%
!! GAGCTAGGAATGGTGGTGGC+TGG - chr4.2:1400392-1400411 MS.gene62678:CDS 60.0%
GAGCCTGAGCCTGAGGAGCT+TGG + chr4.2:1400535-1400554 None:intergenic 65.0%
GCCTGAGCCTGAGGAGCTTG+GGG + chr4.2:1400533-1400552 None:intergenic 65.0%
TCCCCAAGCTCCTCAGGCTC+AGG - chr4.2:1400529-1400548 MS.gene62678:intron 65.0%
! AAGGATGGCGGCTGGTCCTC+AGG - chr4.2:1400082-1400101 MS.gene62678:CDS 65.0%
! AAGGGTGGTGGCTGGTCCTC+AGG - chr4.2:1400046-1400065 MS.gene62678:CDS 65.0%
! GAGCTAGGAAGGATGGCGGC+TGG - chr4.2:1400074-1400093 MS.gene62678:CDS 65.0%
! GAGCTAGGAAGGGTGGTGGC+TGG - chr4.2:1400152-1400171 MS.gene62678:CDS 65.0%
! GAGCTGGGAAGGGTGGTGGC+TGG - chr4.2:1400038-1400057 MS.gene62678:CDS 70.0%
! GGCTGGTCCCCAAGCTCCTC+AGG - chr4.2:1400523-1400542 MS.gene62678:intron 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 1397828 1400652 1397828 ID=MS.gene62678
chr4.2 mRNA 1397828 1400652 1397828 ID=MS.gene62678.t1;Parent=MS.gene62678
chr4.2 exon 1400538 1400652 1400538 ID=MS.gene62678.t1.exon1;Parent=MS.gene62678.t1
chr4.2 CDS 1400538 1400652 1400538 ID=cds.MS.gene62678.t1;Parent=MS.gene62678.t1
chr4.2 exon 1400285 1400434 1400285 ID=MS.gene62678.t1.exon2;Parent=MS.gene62678.t1
chr4.2 CDS 1400285 1400434 1400285 ID=cds.MS.gene62678.t1;Parent=MS.gene62678.t1
chr4.2 exon 1399792 1400184 1399792 ID=MS.gene62678.t1.exon3;Parent=MS.gene62678.t1
chr4.2 CDS 1399792 1400184 1399792 ID=cds.MS.gene62678.t1;Parent=MS.gene62678.t1
chr4.2 exon 1399458 1399534 1399458 ID=MS.gene62678.t1.exon4;Parent=MS.gene62678.t1
chr4.2 CDS 1399458 1399534 1399458 ID=cds.MS.gene62678.t1;Parent=MS.gene62678.t1
chr4.2 exon 1397828 1398958 1397828 ID=MS.gene62678.t1.exon5;Parent=MS.gene62678.t1
chr4.2 CDS 1397828 1398958 1397828 ID=cds.MS.gene62678.t1;Parent=MS.gene62678.t1
Gene Sequence

>MS.gene62678

ATGGATTTTTCCTTTGGGAATGAGTTTCCAGCCAATGTTAAAGTTCTTGTGATTGATCATGATATCGATCTTCTCAATGCTATTGAGAAAACATGGTCTCAATTCAATTACCAAGGTTTGTAAATTAATATATGGTACCTTCTTTGATTGTTAATTGAATTATGTTAACCTATGAAAGTCTCAATCTTTTTGTTACAATTCTTGTTTCATTGTTTCAGTTACTACATGTTCGACAGTTTCTTCAGCCTTGAATTTGATAGCACAAAAGGTGCATTTTGATTTGGTACTACTTGAAGCTGAAATGCCGGACATGGATTCATTTGATTTCTTGCAAAAAATTACACAACAAGTCGATATTCCTGTCATTAGTAAGTAAATATATAATTAATTATATATCATATCACATGATTAATTTATACTTACATGAGATGAGACATTATAATTTATTTATGTTACATATATGTGCAGTGACGATGTGTTCTGAAGGATCAACAAATGGAATAATGAAGGCAATTGAAAATGGAGCTTGTGACTGTTTTGTCAAGCCATTCACTGAAAATCAAGTAAAGTATATGTGGCATCATGCTGTTAGGAAAATGATGAGAGGAAATAAAAAACAAAAAATCAATGAGCAATTGGGGATTGAAGGTAGCCAAATAAGGGCAAGAGATGATTCAAATTTACGCTTAAACGATGTACCATCGGAAAAAGGTGACGAAGATATACCCGAACCACCCCGGACAAAGAAGACAAAGAAGAGAAAGAAGACGAAGAAGACGAAGAAGGAGCGGGTGGTATGGTCTTTAGAATTACATGAGAAATTCATGAATGCTGTTAAAGAACTTGATGCTTCCACTTCCAGTACGCATTCTTTACCTATTCTTGTTTTTATCAAAAGTTTAATGAATTTATCCGTAGTTTACTCTAACATCTAATATGAATCAACTATTTATGCATATCATATCATTAAAGTGACATGTAGCGGAATTTGAAGAGATGATTATACTAGATGATAGTGTAAAATTTGTTTTTGGAAATAAAGTTCAATTTATATTTTTGCAACTTGTGATAACAATTTTTTTCTCTCTCACTCTCTCTCTTTGTTTGTTTTTTTCCAGAGGCGGTACCAAAAACAATTCTTGAACATATGAAGGTGCCTAGACTTACAAGGGAGCAGGTAGCTAGTCATCTTCAGGTAAATTTGTGATAAATCCGTTCTTTGAAATTTAAACTTTTTTTTTTTTTGTCAAAAATGAATAAATAAAGGATTTATAATTGTAAACTAAAAATTTGATTATTTATGGTTTGGAAAATTTGACGGGTATATAGAAAACACATGTCAGTTTGTGACATATTATTAATCTTTTATAGGAATCTCAATATATTTTATGGATTATTAGTCTTTAACTTTCATTCGGATATATATTACCAGTTTACCCTGAAAAGGAGCTAACTCAAAATTTTGATTTTTTGATTTGTTCAGTTATAAAGTTTTCTTAGTCATTCAAATTAAATGCCATAATACTTAATCGAGCAGAAGCTTGTTTTGCATGAAATTAAACATAGATATCAATCTTGTTTCCATACCATTAATTAAACCAAGACAATAAATTGTACACTATTTATTATTTAATGTACACTAACACTAGCATTTTGAAACTGTAATTTATACTGTTATATATTTCGATTTGCAGAAGTATAGACTGTATTTAAATAAACATCAACAAGAACAGGAGCAAGCTCTCATGGTTGTGGAATCCAACAGTTCACATGCTGCTGCTCATGCTGCTGTATTGAAATCATCACAGTGGTCTCACAATTCTCATGTTGCAGAACAACAATATCCAATGGCATATGGTTGCAGTTCTCAACAAGTTCCCAGAAAATTGATACCAAGGCATTTTCCTCATCAGCCCAACACGAACCTGCATCATGGATATGCAACATGGGAGCAGCAATTTCCAAGTGAATATTATATAACTGATCATGATCATGCAGTTGGTCTTGGAAACTTGATGGCGAATAATAGTAATGCATTGCCTCAGCAACAAGAACATCATAATCCACAACAATCACCAATGCATGTTCCTCAGATGAATAATGCAGTCAATGATGTTGTACCGAGTGTGGACAACATCGTGGAAGTGAATAATACTGTAACAGATTTCGGTGAAGCTACAATGCAAGTGGAGGTTGGTCCTCAGGTTCAAGAGCTGGGAAGGGTGGTGGCTGGTCCTCAAGATCAAGAGCTAGGAAGGATGGCGGCTGGTCCTCAGGCTCAAGAGCTAGGAAAGGTGGTGGCTGCTCGTCAGGCTCAACATCAAGAGCTAGGAAGGGTGGTGGCTGGTCCTCAAGCTCAGCCTCAAGAGCTAGGAAGGGTGGTGACTGGTCCTCAGGCTCAAGAGCTAGGAAGGGTGGTGGCTGCTCCACATGCTCAACCTCAAGAGCTAGGAAGGGTGGTTGCTGCTCCACAGGCTCAACCTCAAAAGCTAGGAAGGGTGGTGGCTTGTCCTCAGGCTCAAGAGCTAGGAAGGGTGGTGGCTGGTCCTCAAGCTCAGCCTCAAGAGCTAGGAATGGTGGTGGCTGGTTCTCAGACTCGAAAGCTAAGAAGGGTGGTGGCTGGTCCTCAGGCTCAAGAGCTAGGAAGGGTGGCTGCTGCTCCACATGCTCAACCTAAAAAGCTAGGAAGGGTGGTGGCTGGTCCCCAAGCTCCTCAGGCTCAGGCTCAAGAGCTAGGAAGGGTGGTGGCTACTCCACAGGCTCAGCCTCAAGAGCTAGGAAGGGGAAAGAGGTATAAGAAGCCTAACACAAGATATACTAATTGA
Protein sequence

>MS.gene62678.t1

MDFSFGNEFPANVKVLVIDHDIDLLNAIEKTWSQFNYQVTTCSTVSSALNLIAQKVHFDLVLLEAEMPDMDSFDFLQKITQQVDIPVIMTMCSEGSTNGIMKAIENGACDCFVKPFTENQVKYMWHHAVRKMMRGNKKQKINEQLGIEGSQIRARDDSNLRLNDVPSEKGDEDIPEPPRTKKTKKRKKTKKTKKERVVWSLELHEKFMNAVKELDASTSKAVPKTILEHMKVPRLTREQVASHLQKYRLYLNKHQQEQEQALMVVESNSSHAAAHAAVLKSSQWSHNSHVAEQQYPMAYGCSSQQVPRKLIPRHFPHQPNTNLHHGYATWEQQFPSEYYITDHDHAVGLGNLMANNSNALPQQQEHHNPQQSPMHVPQMNNAVNDVVPSVDNIVEVNNTVTDFGEATMQVEVGPQVQELGRVVAGPQDQELGRMAAGPQAQELGKVVAARQAQHQELGRVVAGPQAQPQELGRVVTGPQAQELGRVVAAPHAQPQELGRVVAAPQAQPQKLGRVVACPQAQELGRVVAGPQAQPQELGMVVAGSQTRKLRRVVAGPQAQELGRVAAAPHAQPKKLGRVVAGPQAPQAQAQELGRVVATPQAQPQELGRGKRYKKPNTRYTN