Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62854.t1 | XP_019454446.1 | 79.1 | 115 | 24 | 0 | 6 | 120 | 100 | 214 | 1.00E-48 | 202.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62854.t1 | Q9FHB2 | 57.0 | 114 | 49 | 0 | 7 | 120 | 89 | 202 | 5.6e-35 | 148.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62854.t1 | A0A4P1R855 | 79.1 | 115 | 24 | 0 | 6 | 120 | 100 | 214 | 7.4e-49 | 202.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene62854.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62854.t1 | MTR_2g102450 | 73.504 | 117 | 31 | 0 | 4 | 120 | 47 | 163 | 1.47e-63 | 197 |
| MS.gene62854.t1 | MTR_2g102450 | 73.504 | 117 | 31 | 0 | 4 | 120 | 101 | 217 | 5.23e-62 | 194 |
| MS.gene62854.t1 | MTR_4g008310 | 66.087 | 115 | 39 | 0 | 6 | 120 | 178 | 292 | 1.15e-51 | 166 |
| MS.gene62854.t1 | MTR_3g101520 | 48.039 | 102 | 53 | 0 | 19 | 120 | 100 | 201 | 1.08e-30 | 109 |
| MS.gene62854.t1 | MTR_3g101520 | 48.039 | 102 | 53 | 0 | 19 | 120 | 108 | 209 | 1.70e-30 | 108 |
| MS.gene62854.t1 | MTR_4g101970 | 80.645 | 31 | 6 | 0 | 90 | 120 | 55 | 85 | 4.39e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62854.t1 | AT5G58280 | 57.018 | 114 | 49 | 0 | 7 | 120 | 89 | 202 | 1.15e-40 | 136 |
| MS.gene62854.t1 | AT5G58280 | 57.018 | 114 | 49 | 0 | 7 | 120 | 121 | 234 | 2.03e-40 | 136 |
| MS.gene62854.t1 | AT5G58280 | 57.018 | 114 | 49 | 0 | 7 | 120 | 122 | 235 | 2.08e-40 | 136 |
| MS.gene62854.t1 | AT3G19184 | 49.020 | 102 | 52 | 0 | 19 | 120 | 104 | 205 | 3.84e-27 | 101 |
| MS.gene62854.t1 | AT3G19184 | 49.020 | 102 | 52 | 0 | 19 | 120 | 104 | 205 | 5.01e-27 | 101 |
| MS.gene62854.t1 | AT5G42700 | 46.078 | 102 | 55 | 0 | 19 | 120 | 59 | 160 | 6.81e-26 | 95.9 |
| MS.gene62854.t1 | AT5G42700 | 46.078 | 102 | 55 | 0 | 19 | 120 | 104 | 205 | 2.00e-25 | 95.9 |
Find 23 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTGCATTGGAACATAAAT+TGG | 0.189949 | 8.1:-21754736 | MS.gene62854:CDS |
| AATATTAGTAACATGGCTAT+TGG | 0.329071 | 8.1:-21755214 | None:intergenic |
| TCTTCCAGGGTCTTCCTAGT+AGG | 0.396253 | 8.1:-21754878 | MS.gene62854:intron |
| ACCATTGACTTGATAAATGA+TGG | 0.398569 | 8.1:+21755076 | None:intergenic |
| GAGTATGATGTTGTCTATAT+TGG | 0.436717 | 8.1:-21754792 | MS.gene62854:CDS |
| GTCTATATTGGCAGTAGAAG+TGG | 0.481140 | 8.1:-21754780 | MS.gene62854:CDS |
| AACAACAAAGAACTCGTGCT+TGG | 0.497832 | 8.1:-21755141 | MS.gene62854:CDS |
| TCCATCATTTATCAAGTCAA+TGG | 0.499589 | 8.1:-21755077 | MS.gene62854:CDS |
| TGTTCCTCACAAAACCTACT+AGG | 0.504504 | 8.1:+21754864 | None:intergenic |
| AAGTCAATGGTCCGGTCTCA+TGG | 0.512045 | 8.1:-21755064 | MS.gene62854:CDS |
| ACAAAGAACTCGTGCTTGGA+AGG | 0.515051 | 8.1:-21755137 | MS.gene62854:CDS |
| GACTCTTTAGTTACGTTGCA+AGG | 0.524631 | 8.1:-21755192 | MS.gene62854:CDS |
| GAAGTGGGCTAAGTGGTGGC+TGG | 0.540038 | 8.1:-21754764 | MS.gene62854:CDS |
| GGGCTAAGTGGTGGCTGGAG+AGG | 0.544907 | 8.1:-21754759 | MS.gene62854:CDS |
| CATTTATCAAGTCAATGGTC+CGG | 0.559658 | 8.1:-21755072 | MS.gene62854:CDS |
| AACAGCTAAAACCATGAGAC+CGG | 0.566641 | 8.1:+21755053 | None:intergenic |
| TTTAGAGGATGAAAATGGCT+CGG | 0.571858 | 8.1:-21754814 | MS.gene62854:CDS |
| TCGTGCTTGGAAGGCTGCAG+AGG | 0.573588 | 8.1:-21755128 | MS.gene62854:CDS |
| TCTATATTGGCAGTAGAAGT+GGG | 0.573838 | 8.1:-21754779 | MS.gene62854:CDS |
| TTGGAACATAAATTGGATGA+TGG | 0.579994 | 8.1:-21754729 | MS.gene62854:CDS |
| GGCAGTAGAAGTGGGCTAAG+TGG | 0.585137 | 8.1:-21754771 | MS.gene62854:CDS |
| TGGCTGGAGAGGATTTGCAT+TGG | 0.612968 | 8.1:-21754748 | MS.gene62854:CDS |
| AGTAGAAGTGGGCTAAGTGG+TGG | 0.694572 | 8.1:-21754768 | MS.gene62854:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GACAAATAAATATAATATTT+TGG | + | chr8.1:21755009-21755028 | None:intergenic | 10.0% |
| !! | TAAAAAGAATGTACAAAACA+TGG | + | chr8.1:21754941-21754960 | None:intergenic | 20.0% |
| !!! | GTTCAGAATATTTTTCTATA+TGG | - | chr8.1:21754900-21754919 | MS.gene62854:intron | 20.0% |
| ! | ATTTGCATTGGAACATAAAT+TGG | - | chr8.1:21755176-21755195 | MS.gene62854:CDS | 25.0% |
| ! | TATTTGTCTTTATTCTTCCA+GGG | - | chr8.1:21755021-21755040 | MS.gene62854:intron | 25.0% |
| ! | TTATTTGTCTTTATTCTTCC+AGG | - | chr8.1:21755020-21755039 | MS.gene62854:intron | 25.0% |
| !!! | ATGATTTTAGAGGATGAAAA+TGG | - | chr8.1:21755093-21755112 | MS.gene62854:CDS | 25.0% |
| GAGTATGATGTTGTCTATAT+TGG | - | chr8.1:21755120-21755139 | MS.gene62854:CDS | 30.0% | |
| TCCATCATTTATCAAGTCAA+TGG | - | chr8.1:21754835-21754854 | MS.gene62854:CDS | 30.0% | |
| TTGGAACATAAATTGGATGA+TGG | - | chr8.1:21755183-21755202 | MS.gene62854:CDS | 30.0% | |
| ! | AGTGCATAACATGATTTTAG+AGG | - | chr8.1:21755083-21755102 | MS.gene62854:CDS | 30.0% |
| ! | ATCATGTTATGCACTGTTTT+TGG | + | chr8.1:21755078-21755097 | None:intergenic | 30.0% |
| !! | ACCATTGACTTGATAAATGA+TGG | + | chr8.1:21754839-21754858 | None:intergenic | 30.0% |
| !!! | GCTTTTTCGATTTCATCTAT+TGG | + | chr8.1:21754746-21754765 | None:intergenic | 30.0% |
| AAACATGGATAAGAATGTGC+AGG | + | chr8.1:21754926-21754945 | None:intergenic | 35.0% | |
| CATTTATCAAGTCAATGGTC+CGG | - | chr8.1:21754840-21754859 | MS.gene62854:CDS | 35.0% | |
| TCTATATTGGCAGTAGAAGT+GGG | - | chr8.1:21755133-21755152 | MS.gene62854:CDS | 35.0% | |
| TTTAGAGGATGAAAATGGCT+CGG | - | chr8.1:21755098-21755117 | MS.gene62854:CDS | 35.0% | |
| !!! | CTCATGGTTTTAGCTGTTTT+TGG | - | chr8.1:21754864-21754883 | MS.gene62854:CDS | 35.0% |
| !!! | TGGTTTTAGCTGTTTTTGGT+TGG | - | chr8.1:21754868-21754887 | MS.gene62854:CDS | 35.0% |
| AACAACAAAGAACTCGTGCT+TGG | - | chr8.1:21754771-21754790 | MS.gene62854:CDS | 40.0% | |
| AACAGCTAAAACCATGAGAC+CGG | + | chr8.1:21754862-21754881 | None:intergenic | 40.0% | |
| GACTCTTTAGTTACGTTGCA+AGG | - | chr8.1:21754720-21754739 | MS.gene62854:CDS | 40.0% | |
| GTCTATATTGGCAGTAGAAG+TGG | - | chr8.1:21755132-21755151 | MS.gene62854:CDS | 40.0% | |
| TGTTCCTCACAAAACCTACT+AGG | + | chr8.1:21755051-21755070 | None:intergenic | 40.0% | |
| !! | TCTTCCTAGTAGGTTTTGTG+AGG | - | chr8.1:21755044-21755063 | MS.gene62854:intron | 40.0% |
| AAAACCTACTAGGAAGACCC+TGG | + | chr8.1:21755041-21755060 | None:intergenic | 45.0% | |
| ACAAAGAACTCGTGCTTGGA+AGG | - | chr8.1:21754775-21754794 | MS.gene62854:CDS | 45.0% | |
| AAGTCAATGGTCCGGTCTCA+TGG | - | chr8.1:21754848-21754867 | MS.gene62854:CDS | 50.0% | |
| TCTTCCAGGGTCTTCCTAGT+AGG | - | chr8.1:21755034-21755053 | MS.gene62854:intron | 50.0% | |
| TGGCTGGAGAGGATTTGCAT+TGG | - | chr8.1:21755164-21755183 | MS.gene62854:CDS | 50.0% | |
| !! | AGTAGAAGTGGGCTAAGTGG+TGG | - | chr8.1:21755144-21755163 | MS.gene62854:CDS | 50.0% |
| !! | GGCAGTAGAAGTGGGCTAAG+TGG | - | chr8.1:21755141-21755160 | MS.gene62854:CDS | 55.0% |
| TCGTGCTTGGAAGGCTGCAG+AGG | - | chr8.1:21754784-21754803 | MS.gene62854:CDS | 60.0% | |
| !! | GAAGTGGGCTAAGTGGTGGC+TGG | - | chr8.1:21755148-21755167 | MS.gene62854:CDS | 60.0% |
| GGGCTAAGTGGTGGCTGGAG+AGG | - | chr8.1:21755153-21755172 | MS.gene62854:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 21754710 | 21755224 | 21754710 | ID=MS.gene62854 |
| chr8.1 | mRNA | 21754710 | 21755224 | 21754710 | ID=MS.gene62854.t1;Parent=MS.gene62854 |
| chr8.1 | exon | 21755045 | 21755224 | 21755045 | ID=MS.gene62854.t1.exon1;Parent=MS.gene62854.t1 |
| chr8.1 | CDS | 21755045 | 21755224 | 21755045 | ID=cds.MS.gene62854.t1;Parent=MS.gene62854.t1 |
| chr8.1 | exon | 21754710 | 21754892 | 21754710 | ID=MS.gene62854.t1.exon2;Parent=MS.gene62854.t1 |
| chr8.1 | CDS | 21754710 | 21754892 | 21754710 | ID=cds.MS.gene62854.t1;Parent=MS.gene62854.t1 |
| Gene Sequence |
| Protein sequence |
