AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62979

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62979.t1 MTR_1g031510 78.125 160 10 2 68 202 477 636 4.39e-73 233
MS.gene62979.t1 MTR_1g031430 65.806 155 28 2 68 197 42 196 1.12e-63 196
MS.gene62979.t1 MTR_1g031280 66.234 154 27 2 68 196 506 659 1.24e-61 203
MS.gene62979.t1 MTR_1g031530 65.772 149 26 2 68 191 190 338 7.64e-61 193
MS.gene62979.t1 MTR_1g031200 65.161 155 28 3 68 197 361 514 2.35e-58 191
MS.gene62979.t1 MTR_1g031540 68.992 129 33 2 68 195 509 631 1.11e-51 177
MS.gene62979.t1 MTR_1g031540 42.735 117 43 1 68 160 156 272 3.64e-22 94.0
MS.gene62979.t1 MTR_1g031540 68.992 129 33 2 68 195 801 923 1.90e-51 178
MS.gene62979.t1 MTR_1g031540 37.748 151 59 4 68 194 448 587 9.89e-23 95.5
MS.gene62979.t1 MTR_1g031560 63.226 155 29 3 68 197 495 646 6.51e-47 163
MS.gene62979.t1 MTR_1g031520 60.759 158 32 3 68 200 474 626 1.81e-46 162
MS.gene62979.t1 MTR_1g031470 66.990 103 31 2 81 182 2 102 8.54e-44 142
MS.gene62979.t1 MTR_1g027160 54.286 140 39 2 68 182 162 301 1.95e-41 143
MS.gene62979.t1 MTR_1g026990 50.943 159 51 4 68 200 162 319 4.24e-40 139
MS.gene62979.t1 MTR_1g027540 52.143 140 42 2 68 182 264 403 7.76e-39 138
MS.gene62979.t1 MTR_1g027250 50.714 140 44 2 68 182 162 301 7.61e-38 133
MS.gene62979.t1 MTR_1g027460 46.835 158 55 3 68 196 189 346 1.38e-37 133
MS.gene62979.t1 MTR_1g027320 49.265 136 44 2 68 178 162 297 1.56e-37 132
MS.gene62979.t1 MTR_1g027070 48.171 164 52 5 68 202 558 717 3.23e-37 137
MS.gene62979.t1 MTR_1g027830 48.447 161 57 3 68 202 515 675 3.95e-37 136
MS.gene62979.t1 MTR_6g045030 47.468 158 56 4 68 200 268 423 3.98e-37 133
MS.gene62979.t1 MTR_1g027840 47.826 161 58 3 68 202 188 348 4.20e-37 132
MS.gene62979.t1 MTR_1g027930 47.205 161 59 3 68 202 132 292 5.39e-37 130
MS.gene62979.t1 MTR_1g028280 50.370 135 42 2 68 177 533 667 5.51e-37 136
MS.gene62979.t1 MTR_1g028280 50.370 135 42 2 68 177 494 628 7.92e-37 135
MS.gene62979.t1 MTR_1g027730 47.297 148 53 2 68 190 188 335 1.13e-35 128
MS.gene62979.t1 MTR_1g027140 46.341 164 55 5 68 202 592 751 1.54e-35 132
MS.gene62979.t1 MTR_3g028630 46.897 145 52 2 60 179 251 395 4.77e-35 128
MS.gene62979.t1 MTR_1g027440 55.000 120 38 3 68 173 440 557 5.54e-35 130
MS.gene62979.t1 MTR_1g027050 45.732 164 56 5 68 202 595 754 1.33e-34 129
MS.gene62979.t1 MTR_1g028100 47.712 153 53 5 68 194 249 400 1.65e-33 124
MS.gene62979.t1 MTR_1g028170 47.059 153 54 4 68 194 276 427 1.93e-33 124
MS.gene62979.t1 MTR_1g027410 50.735 136 42 2 68 178 261 396 2.64e-33 123
MS.gene62979.t1 MTR_1g027680 44.667 150 53 4 68 191 552 697 1.76e-32 123
MS.gene62979.t1 MTR_1g027990 42.949 156 63 3 68 197 555 710 2.20e-32 123
MS.gene62979.t1 MTR_4g073220 53.125 128 36 3 68 174 475 599 4.18e-32 122
MS.gene62979.t1 MTR_1g027030 42.331 163 61 4 68 197 594 756 4.43e-31 119
MS.gene62979.t1 MTR_1g028020 46.104 154 54 5 69 196 308 458 7.12e-31 119
MS.gene62979.t1 MTR_1g027030 42.331 163 61 4 68 197 544 706 7.31e-31 119
MS.gene62979.t1 MTR_3g028650 43.651 126 46 2 68 168 419 544 1.67e-27 108
MS.gene62979.t1 MTR_1g027200 49.558 113 41 3 68 164 393 505 2.42e-26 105
MS.gene62979.t1 MTR_1g027690 42.857 119 45 1 68 163 448 566 4.25e-26 105
MS.gene62979.t1 MTR_1g027060 42.623 122 45 2 68 164 366 487 1.29e-25 103
MS.gene62979.t1 MTR_1g028290 45.082 122 42 2 68 164 449 570 1.34e-25 103
MS.gene62979.t1 MTR_1g027820 43.089 123 44 3 68 164 417 539 3.13e-25 102
MS.gene62979.t1 MTR_1g027040 42.276 123 45 2 68 164 421 543 7.26e-25 101
MS.gene62979.t1 MTR_1g108910 45.614 114 45 4 69 169 203 312 1.06e-24 99.0
MS.gene62979.t1 MTR_1g027420 42.520 127 46 3 68 168 407 532 1.13e-24 100
MS.gene62979.t1 MTR_4g073230 41.803 122 46 2 68 164 459 580 1.25e-24 100
MS.gene62979.t1 MTR_1g028220 42.063 126 48 3 68 168 421 546 1.36e-24 100
MS.gene62979.t1 MTR_7g085860 40.602 133 54 4 69 176 326 458 1.86e-24 99.8
MS.gene62979.t1 MTR_1g110260 42.520 127 47 4 69 169 395 521 2.74e-24 99.8
MS.gene62979.t1 MTR_6g083290 42.969 128 47 4 68 169 176 303 3.79e-24 97.4
MS.gene62979.t1 MTR_1g027700 40.164 122 48 2 68 164 194 315 4.55e-24 97.4
MS.gene62979.t1 MTR_6g082830 41.803 122 46 3 70 166 166 287 7.05e-24 96.3
MS.gene62979.t1 MTR_1g027960 40.164 122 48 2 68 164 438 559 7.32e-24 98.6
MS.gene62979.t1 MTR_1g026940 40.476 126 49 3 68 168 463 587 1.06e-23 98.2
MS.gene62979.t1 MTR_1g110220 41.985 131 42 5 69 169 415 541 1.58e-23 97.8
MS.gene62979.t1 MTR_1g027970 40.870 115 45 1 68 159 450 564 1.72e-23 97.8
MS.gene62979.t1 MTR_1g027150 40.164 122 48 2 68 164 419 540 3.76e-23 96.7
MS.gene62979.t1 MTR_8g104520 37.226 137 55 4 70 175 506 642 4.12e-23 96.7
MS.gene62979.t1 MTR_1g027890 39.669 121 49 3 68 164 263 383 4.64e-23 95.5
MS.gene62979.t1 MTR_6g083300 41.007 139 53 5 71 181 167 304 4.66e-23 94.4
MS.gene62979.t1 MTR_6g083210 42.400 125 46 4 71 169 167 291 5.17e-23 94.0
MS.gene62979.t1 MTR_6g082860 41.270 126 41 3 70 166 222 343 5.47e-23 94.7
MS.gene62979.t1 MTR_1g027500 43.363 113 38 2 68 156 464 574 6.01e-23 96.3
MS.gene62979.t1 MTR_1g027370 38.095 147 59 3 68 191 464 601 7.57e-23 95.9
MS.gene62979.t1 MTR_6g083270 43.443 122 43 4 71 166 322 443 8.01e-23 95.1
MS.gene62979.t1 MTR_1g028130 40.164 122 48 2 68 164 450 571 8.72e-23 95.5
MS.gene62979.t1 MTR_1g031400 61.039 77 6 1 68 120 180 256 1.77e-22 92.0
MS.gene62979.t1 MTR_0690s0010 39.344 122 48 3 68 164 398 518 3.43e-22 94.0
MS.gene62979.t1 MTR_1g028080 39.344 122 49 2 68 164 450 571 3.92e-22 93.6
MS.gene62979.t1 MTR_1g110140 36.552 145 53 5 69 186 217 349 3.96e-22 92.4
MS.gene62979.t1 MTR_1g031580 42.735 117 43 1 68 160 461 577 4.33e-22 93.6
MS.gene62979.t1 MTR_1g031460 41.880 117 44 1 68 160 452 568 6.02e-22 93.2
MS.gene62979.t1 MTR_6g445600 39.669 121 49 3 68 164 424 544 6.26e-22 93.2
MS.gene62979.t1 MTR_7g082280 38.806 134 51 4 72 176 591 722 7.65e-22 92.8
MS.gene62979.t1 MTR_6g083760 43.089 123 42 4 71 166 487 608 1.07e-21 92.4
MS.gene62979.t1 MTR_7g082400 38.060 134 56 3 69 175 245 378 1.27e-21 91.7
MS.gene62979.t1 MTR_6g082870 40.984 122 46 3 71 166 524 645 1.34e-21 92.4
MS.gene62979.t1 MTR_6g082950 42.276 123 43 4 71 166 287 408 2.79e-21 90.9
MS.gene62979.t1 MTR_1g031320 41.026 117 45 1 68 160 465 581 2.82e-21 91.3
MS.gene62979.t1 MTR_7g082380 37.956 137 57 4 69 177 222 358 3.41e-21 90.1
MS.gene62979.t1 MTR_7g082300 38.235 136 54 4 69 176 711 844 4.08e-21 90.9
MS.gene62979.t1 MTR_6g083780 40.602 133 54 4 68 175 513 645 4.09e-21 90.9
MS.gene62979.t1 MTR_1g110270 36.000 150 68 4 69 194 483 628 4.28e-21 90.9
MS.gene62979.t1 MTR_7g082430 38.000 150 61 5 69 192 476 619 7.76e-21 90.1
MS.gene62979.t1 MTR_1g031340 46.316 95 34 2 103 197 1 78 9.87e-21 82.4
MS.gene62979.t1 MTR_7g082470 36.567 134 58 3 69 175 495 628 1.06e-20 89.7
MS.gene62979.t1 MTR_7g082490 41.593 113 41 3 69 156 272 384 2.33e-20 87.8
MS.gene62979.t1 MTR_4g044393 42.188 128 48 5 69 170 695 822 3.15e-20 88.2
MS.gene62979.t1 MTR_7g082360 39.098 133 53 5 69 175 199 329 1.18e-19 85.5
MS.gene62979.t1 MTR_0394s0020 36.719 128 55 3 69 170 235 362 1.46e-18 82.8
MS.gene62979.t1 MTR_7g082110 40.708 113 42 3 69 156 697 809 6.94e-18 81.6
MS.gene62979.t1 MTR_1g110160 39.048 105 45 4 80 169 466 566 6.13e-15 72.8
MS.gene62979.t1 MTR_5g006160 34.351 131 62 3 60 168 643 771 1.00e-14 72.4
MS.gene62979.t1 MTR_2g067970 33.088 136 62 4 62 170 758 891 1.13e-14 72.4
MS.gene62979.t1 MTR_1g027920 55.000 60 26 1 113 171 34 93 1.17e-14 67.0
MS.gene62979.t1 MTR_7g060570 37.692 130 50 6 72 174 504 629 2.44e-14 71.2
MS.gene62979.t1 MTR_4g113710 31.875 160 71 5 53 178 254 409 3.09e-14 70.9
MS.gene62979.t1 MTR_2g036500 38.400 125 43 5 68 164 234 352 4.15e-14 70.1
MS.gene62979.t1 MTR_2g067990 30.882 136 65 3 62 170 870 1003 2.98e-13 68.2
MS.gene62979.t1 MTR_4g126930 31.967 122 54 3 61 155 288 407 3.42e-13 67.8
MS.gene62979.t1 MTR_5g013070 34.848 132 54 6 62 165 665 792 5.96e-13 67.4
MS.gene62979.t1 MTR_6g083740 36.792 106 41 4 87 166 122 227 5.96e-13 65.9
MS.gene62979.t1 MTR_1g104555 30.714 140 55 4 61 164 536 669 6.43e-13 67.0
MS.gene62979.t1 MTR_1g031220 43.939 66 34 1 68 133 30 92 8.55e-13 62.0
MS.gene62979.t1 MTR_6g043790 31.783 129 59 3 68 169 654 780 9.04e-13 66.6
MS.gene62979.t1 MTR_2g064930 30.921 152 73 6 65 190 205 350 1.67e-12 65.5
MS.gene62979.t1 MTR_8g461260 33.333 123 54 3 65 161 203 323 1.67e-12 65.5
MS.gene62979.t1 MTR_8g445800 28.125 160 88 2 60 194 851 1008 1.75e-12 65.9
MS.gene62979.t1 MTR_5g091380 33.121 157 58 7 65 195 340 475 1.80e-12 65.5
MS.gene62979.t1 MTR_8g445800 28.125 160 88 2 60 194 653 810 2.03e-12 65.9
MS.gene62979.t1 MTR_8g445800 28.125 160 88 2 60 194 706 863 2.03e-12 65.9
MS.gene62979.t1 MTR_0049s0070 31.148 122 55 3 65 159 392 511 2.08e-12 65.5
MS.gene62979.t1 MTR_3g087060 33.333 120 50 4 68 159 436 553 2.85e-12 65.1
MS.gene62979.t1 MTR_6g043510 34.375 128 47 5 68 164 649 770 3.04e-12 65.1
MS.gene62979.t1 MTR_3g087060 33.333 120 50 4 68 159 447 564 4.18e-12 64.7
MS.gene62979.t1 MTR_2g064940 33.333 117 50 3 65 155 200 314 4.95e-12 64.3
MS.gene62979.t1 MTR_7g018200 31.967 122 54 3 65 159 460 579 5.82e-12 64.3
MS.gene62979.t1 MTR_4g114250 36.364 121 51 4 68 164 686 804 6.22e-12 64.3
MS.gene62979.t1 MTR_4g105540 30.625 160 84 5 61 195 479 636 8.10e-12 63.9
MS.gene62979.t1 MTR_2g008360 32.520 123 53 4 65 159 451 571 9.05e-12 63.5
MS.gene62979.t1 MTR_0049s0070 31.148 122 55 3 65 159 455 574 9.15e-12 63.5
MS.gene62979.t1 MTR_4g123880 31.325 166 67 8 65 197 336 487 1.35e-11 63.2
MS.gene62979.t1 MTR_2g036430 31.818 132 62 3 68 173 218 347 1.36e-11 62.8
MS.gene62979.t1 MTR_7g033135 33.846 130 59 5 62 166 468 595 1.65e-11 62.8
MS.gene62979.t1 MTR_8g059615 35.606 132 57 5 57 162 792 921 2.28e-11 62.8
MS.gene62979.t1 MTR_1g097160 32.520 123 53 3 65 159 462 582 2.36e-11 62.4
MS.gene62979.t1 MTR_3g047890 34.167 120 51 4 65 158 338 455 2.42e-11 62.4
MS.gene62979.t1 MTR_6g016495 31.148 122 55 3 65 159 458 577 2.62e-11 62.4
MS.gene62979.t1 MTR_8g058250 32.836 134 62 3 57 164 717 848 3.55e-11 62.0
MS.gene62979.t1 MTR_8g095030 34.426 122 51 5 65 159 431 550 4.00e-11 61.6
MS.gene62979.t1 MTR_4g081640 31.126 151 75 5 72 196 632 779 4.14e-11 62.0
MS.gene62979.t1 MTR_5g013130 34.677 124 51 5 62 157 662 783 4.28e-11 61.6
MS.gene62979.t1 MTR_8g058250 32.836 134 62 3 57 164 813 944 4.48e-11 61.6
MS.gene62979.t1 MTR_7g111690 33.333 120 52 4 65 158 352 469 6.44e-11 61.2
MS.gene62979.t1 MTR_4g130210 34.426 122 51 4 65 159 383 502 6.92e-11 61.2
MS.gene62979.t1 MTR_4g123880 32.500 120 53 4 65 158 336 453 9.67e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62979.t1 AT5G38240 40.881 159 58 5 66 195 469 620 4.07e-26 105
MS.gene62979.t1 AT5G38240 40.881 159 58 5 66 195 487 638 4.15e-26 105
MS.gene62979.t1 AT5G38250 40.506 158 59 4 66 195 466 616 4.73e-26 105
MS.gene62979.t1 AT5G38240 40.881 159 58 5 66 195 423 574 5.77e-25 102
MS.gene62979.t1 AT1G67000 36.667 180 67 8 66 202 715 890 2.23e-24 100
MS.gene62979.t1 AT5G38280 38.255 149 58 5 71 187 493 639 3.71e-24 99.8
MS.gene62979.t1 AT5G38280 38.255 149 58 5 71 187 493 639 8.55e-24 98.6
MS.gene62979.t1 AT5G39030 42.063 126 44 3 70 166 658 783 8.55e-24 98.6
MS.gene62979.t1 AT5G39030 42.063 126 44 3 70 166 652 777 9.22e-24 98.6
MS.gene62979.t1 AT1G66910 35.000 160 69 5 66 195 506 660 3.11e-22 94.0
MS.gene62979.t1 AT1G66910 35.000 160 69 5 66 195 503 657 5.47e-22 93.6
MS.gene62979.t1 AT5G39020 40.650 123 46 3 71 166 657 779 8.50e-21 90.1
MS.gene62979.t1 AT1G66920 35.714 140 61 3 66 178 458 595 3.27e-20 88.2
MS.gene62979.t1 AT1G66920 35.714 140 61 3 66 178 466 603 3.28e-20 88.2
MS.gene62979.t1 AT1G66920 35.294 136 61 2 70 178 442 577 5.81e-20 87.4
MS.gene62979.t1 AT5G38260 34.014 147 65 5 70 184 481 627 3.82e-19 85.1
MS.gene62979.t1 AT1G34300 31.210 157 74 5 62 190 637 787 1.83e-14 71.6
MS.gene62979.t1 AT1G29720 33.766 154 73 5 65 191 828 979 4.29e-14 70.5
MS.gene62979.t1 AT1G70530 27.586 174 88 7 61 200 475 644 8.62e-13 66.6
MS.gene62979.t1 AT2G23950 34.646 127 54 4 60 159 448 572 1.03e-12 66.6
MS.gene62979.t1 AT1G71830 32.520 123 53 3 65 159 460 580 2.45e-12 65.5
MS.gene62979.t1 AT3G53590 34.109 129 62 5 49 156 765 891 4.01e-12 64.7
MS.gene62979.t1 AT1G60800 33.607 122 52 4 65 159 392 511 4.46e-12 64.7
MS.gene62979.t1 AT1G60800 33.607 122 52 3 65 159 459 578 6.71e-12 64.3
MS.gene62979.t1 AT4G30520 34.426 122 51 3 65 159 457 576 1.19e-11 63.5
MS.gene62979.t1 AT1G56130 32.258 124 57 3 60 158 844 965 1.29e-11 63.5
MS.gene62979.t1 AT4G32300 36.066 122 49 4 62 156 646 765 1.35e-11 63.2
MS.gene62979.t1 AT1G56130 30.645 124 59 2 60 158 696 817 1.91e-11 62.8
MS.gene62979.t1 AT1G56130 30.645 124 59 2 60 158 645 766 2.08e-11 62.8
MS.gene62979.t1 AT4G33430 30.894 123 55 3 65 159 494 614 2.98e-11 62.4
MS.gene62979.t1 AT1G56140 29.839 124 60 2 60 158 843 964 3.79e-11 62.0
MS.gene62979.t1 AT1G34210 30.894 123 55 3 65 159 463 583 3.94e-11 62.0
MS.gene62979.t1 AT1G34210 30.894 123 55 3 65 159 463 583 3.94e-11 62.0
MS.gene62979.t1 AT4G33430 30.894 123 55 3 65 159 447 567 4.60e-11 61.6
MS.gene62979.t1 AT1G53420 33.333 126 56 3 57 156 773 896 4.70e-11 61.6
MS.gene62979.t1 AT1G56120 29.268 164 85 6 60 198 842 999 5.13e-11 61.6
MS.gene62979.t1 AT1G56120 29.268 164 85 6 60 198 860 1017 5.81e-11 61.6
MS.gene62979.t1 AT4G01330 28.302 159 85 4 65 197 319 474 6.78e-11 61.2
MS.gene62979.t1 AT1G56120 29.268 164 85 6 60 198 694 851 6.91e-11 61.2
MS.gene62979.t1 AT5G18500 31.707 123 56 3 62 158 320 440 9.21e-11 60.8
MS.gene62979.t1 AT5G18500 31.707 123 56 3 62 158 320 440 9.21e-11 60.8
MS.gene62979.t1 AT5G18500 31.707 123 56 3 62 158 320 440 9.21e-11 60.8
MS.gene62979.t1 AT5G18500 31.707 123 56 3 62 158 320 440 9.21e-11 60.8
MS.gene62979.t1 AT5G18500 31.707 123 56 3 62 158 320 440 9.21e-11 60.8
MS.gene62979.t1 AT5G18500 31.707 123 56 3 62 158 320 440 9.21e-11 60.8

Find 43 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGATGATTGTGGCTTTA+TGG 0.201342 1.3:-16687080 MS.gene62979:CDS
ACATTTCTGTACAATAATTC+AGG 0.248854 1.3:+16687305 None:intergenic
AAATGATGACCAATGGTATT+TGG 0.303676 1.3:-16687784 MS.gene62979:CDS
GGATAAAGGTAAAGCTTATT+AGG 0.306141 1.3:+16686976 None:intergenic
TACATCAATAAATGATTTCA+TGG 0.372631 1.3:-16686890 MS.gene62979:CDS
TTAATCACTTTATCCATTGA+AGG 0.380286 1.3:+16687030 None:intergenic
GACCAATGGTATTTGGATAC+AGG 0.384369 1.3:-16687777 MS.gene62979:intron
ATCAGATTCATCTTCATTGT+AGG 0.390760 1.3:+16687845 None:intergenic
AAAGCTGATGTCTATAGCTT+TGG 0.404244 1.3:-16687266 MS.gene62979:CDS
TAGCTTTGGAATGTTGTTGA+TGG 0.428515 1.3:-16687252 MS.gene62979:CDS
ATTAAGATGCTTGAAGAAGA+TGG 0.430170 1.3:-16687012 MS.gene62979:CDS
TCCATTGAAGGACGATCCTC+GGG 0.453361 1.3:+16687042 None:intergenic
ATCCATTGAAGGACGATCCT+CGG 0.459503 1.3:+16687041 None:intergenic
CTGGTAGATCTTGTGGATAA+AGG 0.466742 1.3:+16686962 None:intergenic
TTAAGATGCTTGAAGAAGAT+GGG 0.468545 1.3:-16687011 MS.gene62979:CDS
ACCCGAGGATCGTCCTTCAA+TGG 0.480221 1.3:-16687043 MS.gene62979:CDS
TCCTCAGCTGGTAGATCTTG+TGG 0.481056 1.3:+16686955 None:intergenic
TGGAATGTTGTTGATGGAGA+TGG 0.498367 1.3:-16687246 MS.gene62979:CDS
TTATTGATGGCTACTATCAA+AGG 0.503980 1.3:-16687810 MS.gene62979:CDS
AAGAGGAACCATTGGATACA+TGG 0.506102 1.3:-16687330 MS.gene62979:CDS
AGCTGAATTCAAGTCTAAGA+AGG 0.514114 1.3:-16687901 MS.gene62979:CDS
TCATGGTGAAGGTTCAATCA+AGG 0.516539 1.3:-16688048 MS.gene62979:CDS
CATTTCTGTACAATAATTCA+GGG 0.517571 1.3:+16687306 None:intergenic
AAGAAGAAAGTGCAGAAGGC+AGG 0.524370 1.3:-16688007 MS.gene62979:intron
ATTTATGATCAGTTACATGA+TGG 0.538012 1.3:-16687156 MS.gene62979:intron
GAATTATTGTACAGAAATGT+CGG 0.540216 1.3:-16687302 MS.gene62979:CDS
ACAGCAGCAAGAGGAACCAT+TGG 0.542287 1.3:-16687338 MS.gene62979:intron
CGCAAAGAAAATGATGATTG+TGG 0.548717 1.3:-16687088 MS.gene62979:CDS
ATCATTTATTGATGTATCAG+AGG 0.556428 1.3:+16686897 None:intergenic
TTATCTCTAAAATCCTCAGC+TGG 0.560356 1.3:+16686943 None:intergenic
TCAAAAGAAGAAAGTGCAGA+AGG 0.572705 1.3:-16688011 MS.gene62979:CDS
AACCTGTATCCAAATACCAT+TGG 0.580874 1.3:+16687775 None:intergenic
GAAGATGAATCTGATGCTGA+TGG 0.584045 1.3:-16687837 MS.gene62979:CDS
AGCTCCAAGAATCATGGTGA+AGG 0.585421 1.3:-16688059 None:intergenic
ATAATAATACTAGAAGTTCG+CGG 0.588869 1.3:-16686924 MS.gene62979:CDS
TCCACAAGATCTACCAGCTG+AGG 0.594323 1.3:-16686956 MS.gene62979:CDS
TAAGATGCTTGAAGAAGATG+GGG 0.594780 1.3:-16687010 MS.gene62979:CDS
TTCAGGGGCCATGTATCCAA+TGG 0.595201 1.3:+16687322 None:intergenic
AAGGTGAAAATGATGACCAA+TGG 0.606579 1.3:-16687791 MS.gene62979:CDS
GATTCATTAACAGCAGCAAG+AGG 0.606622 1.3:-16687347 MS.gene62979:intron
CATGGTGAAGGTTCAATCAA+GGG 0.616102 1.3:-16688047 MS.gene62979:CDS
GTGTATACAAACAAAACCCG+AGG 0.678911 1.3:-16687058 MS.gene62979:CDS
ATTTCTGTACAATAATTCAG+GGG 0.690349 1.3:+16687307 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACATCAATAAATGATTTCA+TGG - chr1.3:16688035-16688054 MS.gene62979:CDS 20.0%
!!! CTTTTATTTATTTTCAAGCA+CGG - chr1.3:16687484-16687503 MS.gene62979:intron 20.0%
! AACTGATCATAAATCCAAAA+AGG + chr1.3:16687763-16687782 None:intergenic 25.0%
! ACATTTCTGTACAATAATTC+AGG + chr1.3:16687623-16687642 None:intergenic 25.0%
! ACTGATCATAAATCCAAAAA+GGG + chr1.3:16687762-16687781 None:intergenic 25.0%
! ATAATAATACTAGAAGTTCG+CGG - chr1.3:16688001-16688020 MS.gene62979:intron 25.0%
! ATCATTTATTGATGTATCAG+AGG + chr1.3:16688031-16688050 None:intergenic 25.0%
! ATTTATGATCAGTTACATGA+TGG - chr1.3:16687769-16687788 MS.gene62979:intron 25.0%
! ATTTCTGTACAATAATTCAG+GGG + chr1.3:16687621-16687640 None:intergenic 25.0%
! CATTTCTGTACAATAATTCA+GGG + chr1.3:16687622-16687641 None:intergenic 25.0%
! GAATTATTGTACAGAAATGT+CGG - chr1.3:16687623-16687642 MS.gene62979:intron 25.0%
! TCCAAAAAGGGAAATAAATT+TGG + chr1.3:16687750-16687769 None:intergenic 25.0%
! TTAATCACTTTATCCATTGA+AGG + chr1.3:16687898-16687917 None:intergenic 25.0%
! TTAGAAAGGTTAAGATTCAA+AGG - chr1.3:16687281-16687300 MS.gene62979:CDS 25.0%
AAAAGGTTATACCATGAAGA+TGG - chr1.3:16687390-16687409 MS.gene62979:intron 30.0%
AAATGATGACCAATGGTATT+TGG - chr1.3:16687141-16687160 MS.gene62979:CDS 30.0%
AACATTATGGTTAACGCAAA+AGG - chr1.3:16687256-16687275 MS.gene62979:CDS 30.0%
AAGATTTGCATGCAACATTA+TGG - chr1.3:16687243-16687262 MS.gene62979:CDS 30.0%
ACATTATGGTTAACGCAAAA+GGG - chr1.3:16687257-16687276 MS.gene62979:CDS 30.0%
ATCAGATTCATCTTCATTGT+AGG + chr1.3:16687083-16687102 None:intergenic 30.0%
ATTAAGATGCTTGAAGAAGA+TGG - chr1.3:16687913-16687932 MS.gene62979:intron 30.0%
GGATAAAGGTAAAGCTTATT+AGG + chr1.3:16687952-16687971 None:intergenic 30.0%
GTTAAGATTCAAAGGAATGA+TGG - chr1.3:16687289-16687308 MS.gene62979:CDS 30.0%
TTAAGATGCTTGAAGAAGAT+GGG - chr1.3:16687914-16687933 MS.gene62979:intron 30.0%
TTTCTACCCTTCGTAATTAT+TGG + chr1.3:16686945-16686964 None:intergenic 30.0%
! AAATGATGATTGTGGCTTTA+TGG - chr1.3:16687845-16687864 MS.gene62979:CDS 30.0%
! AATGAAGGTGTATAATAGCA+AGG - chr1.3:16687423-16687442 MS.gene62979:intron 30.0%
! ATGAAGGTGTATAATAGCAA+GGG - chr1.3:16687424-16687443 MS.gene62979:intron 30.0%
! GCCAAATTTATTTCCCTTTT+TGG - chr1.3:16687746-16687765 MS.gene62979:intron 30.0%
! TTATTGATGGCTACTATCAA+AGG - chr1.3:16687115-16687134 MS.gene62979:CDS 30.0%
!! CAAGGGTAGATTGATTTTAA+AGG - chr1.3:16687441-16687460 MS.gene62979:intron 30.0%
AAAGCTGATGTCTATAGCTT+TGG - chr1.3:16687659-16687678 MS.gene62979:intron 35.0%
AACCTGTATCCAAATACCAT+TGG + chr1.3:16687153-16687172 None:intergenic 35.0%
AAGGTGAAAATGATGACCAA+TGG - chr1.3:16687134-16687153 MS.gene62979:CDS 35.0%
AATGATATAGCTGTGAGAAG+CGG - chr1.3:16686990-16687009 MS.gene62979:CDS 35.0%
AGCTGAATTCAAGTCTAAGA+AGG - chr1.3:16687024-16687043 MS.gene62979:CDS 35.0%
CGCAAAGAAAATGATGATTG+TGG - chr1.3:16687837-16687856 MS.gene62979:CDS 35.0%
CTTTGACAGTTGCTTGAAAA+AGG - chr1.3:16687373-16687392 MS.gene62979:intron 35.0%
TAACGCAAAAGGGATTAGAA+AGG - chr1.3:16687267-16687286 MS.gene62979:CDS 35.0%
TAAGATGCTTGAAGAAGATG+GGG - chr1.3:16687915-16687934 MS.gene62979:intron 35.0%
TGGTACAGAATCATTCATCT+CGG - chr1.3:16687309-16687328 MS.gene62979:CDS 35.0%
! TCAAAAGAAGAAAGTGCAGA+AGG - chr1.3:16686914-16686933 MS.gene62979:CDS 35.0%
!! TAGCTTTGGAATGTTGTTGA+TGG - chr1.3:16687673-16687692 MS.gene62979:intron 35.0%
!! TTATCTCTAAAATCCTCAGC+TGG + chr1.3:16687985-16688004 None:intergenic 35.0%
!! TTATTTTCAAGCACGGTCTA+AGG - chr1.3:16687491-16687510 MS.gene62979:intron 35.0%
!!! GATTTTAAAGGCACCATTGT+CGG - chr1.3:16687453-16687472 MS.gene62979:intron 35.0%
!!! TGCTGATGGAGTTTTATTGA+TGG - chr1.3:16687102-16687121 MS.gene62979:CDS 35.0%
AAGAGGAACCATTGGATACA+TGG - chr1.3:16687595-16687614 MS.gene62979:intron 40.0%
ACATGAGTGGCAGGAAAAAT+TGG - chr1.3:16687182-16687201 MS.gene62979:intron 40.0%
ATGATATAGCTGTGAGAAGC+GGG - chr1.3:16686991-16687010 MS.gene62979:CDS 40.0%
CTGGTAGATCTTGTGGATAA+AGG + chr1.3:16687966-16687985 None:intergenic 40.0%
GAAGATGAATCTGATGCTGA+TGG - chr1.3:16687088-16687107 MS.gene62979:CDS 40.0%
GACCAATGGTATTTGGATAC+AGG - chr1.3:16687148-16687167 MS.gene62979:intron 40.0%
GAGGAAGAACTTGAATGCAT+TGG - chr1.3:16687711-16687730 MS.gene62979:intron 40.0%
GATTCATTAACAGCAGCAAG+AGG - chr1.3:16687578-16687597 MS.gene62979:intron 40.0%
GCAGGTCCAATAATTACGAA+GGG - chr1.3:16686936-16686955 MS.gene62979:CDS 40.0%
GGTTTCTCAAATCACATGAG+TGG - chr1.3:16687169-16687188 MS.gene62979:intron 40.0%
GTGTATACAAACAAAACCCG+AGG - chr1.3:16687867-16687886 MS.gene62979:CDS 40.0%
! TGGAATGTTGTTGATGGAGA+TGG - chr1.3:16687679-16687698 MS.gene62979:intron 40.0%
!! AAAAGTGCTATCACCGACAA+TGG + chr1.3:16687469-16687488 None:intergenic 40.0%
ATCCATTGAAGGACGATCCT+CGG + chr1.3:16687887-16687906 None:intergenic 45.0%
GGCAGGTCCAATAATTACGA+AGG - chr1.3:16686935-16686954 MS.gene62979:CDS 45.0%
TCTCAAATCACATGAGTGGC+AGG - chr1.3:16687173-16687192 MS.gene62979:intron 45.0%
TGATATAGCTGTGAGAAGCG+GGG - chr1.3:16686992-16687011 MS.gene62979:CDS 45.0%
TGCACATGCTTCCATCTTCA+TGG + chr1.3:16687404-16687423 None:intergenic 45.0%
! AAGAAGAAAGTGCAGAAGGC+AGG - chr1.3:16686918-16686937 MS.gene62979:CDS 45.0%
! GATGGAAGCATGTGCAATGA+AGG - chr1.3:16687408-16687427 MS.gene62979:intron 45.0%
ACAGCAGCAAGAGGAACCAT+TGG - chr1.3:16687587-16687606 MS.gene62979:intron 50.0%
TCCACAAGATCTACCAGCTG+AGG - chr1.3:16687969-16687988 MS.gene62979:intron 50.0%
TCCATTGAAGGACGATCCTC+GGG + chr1.3:16687886-16687905 None:intergenic 50.0%
TCCTCAGCTGGTAGATCTTG+TGG + chr1.3:16687973-16687992 None:intergenic 50.0%
TTCAGGGGCCATGTATCCAA+TGG + chr1.3:16687606-16687625 None:intergenic 50.0%
TTGATGGAGATGGCGCAAGT+AGG - chr1.3:16687689-16687708 MS.gene62979:intron 50.0%
ACCCGAGGATCGTCCTTCAA+TGG - chr1.3:16687882-16687901 MS.gene62979:CDS 55.0%
ATGGAGATGGCGCAAGTAGG+AGG - chr1.3:16687692-16687711 MS.gene62979:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 16686879 16688068 16686879 ID=MS.gene62979
chr1.3 mRNA 16686879 16688068 16686879 ID=MS.gene62979.t1;Parent=MS.gene62979
chr1.3 exon 16688008 16688068 16688008 ID=MS.gene62979.t1.exon1;Parent=MS.gene62979.t1
chr1.3 CDS 16688008 16688068 16688008 ID=cds.MS.gene62979.t1;Parent=MS.gene62979.t1
chr1.3 exon 16687778 16687921 16687778 ID=MS.gene62979.t1.exon2;Parent=MS.gene62979.t1
chr1.3 CDS 16687778 16687921 16687778 ID=cds.MS.gene62979.t1;Parent=MS.gene62979.t1
chr1.3 exon 16687241 16687356 16687241 ID=MS.gene62979.t1.exon3;Parent=MS.gene62979.t1
chr1.3 CDS 16687241 16687356 16687241 ID=cds.MS.gene62979.t1;Parent=MS.gene62979.t1
chr1.3 exon 16686879 16687166 16686879 ID=MS.gene62979.t1.exon4;Parent=MS.gene62979.t1
chr1.3 CDS 16686879 16687166 16686879 ID=cds.MS.gene62979.t1;Parent=MS.gene62979.t1
Gene Sequence

>MS.gene62979

ATGGTGAAGGTTCAATCAAGGGTCAAGAACATAGCTCAAAAGAAGAAAGTGCAGAAGGCAGGTCCAATAATTACGAAGGGTAGAAAAAGAAAGTTGAGAAGAAATACATTCAATGATATAGCTGTGAGAAGCGGGGTCATTTTGCAGCTGAATTCAAGTCTAAGAAGGTTGCAAGAGATAGAGCAGATGAAGAACAGTTTTCCTACAATGAAGATGAATCTGATGCTGATGGAGTTTTATTGATGGCTACTATCAAAGGTGAAAATGATGACCAATGGTATTTGGATACAGGTTTCTCAAATCACATGAGTGGCAGGAAAAATTGGTTTTATATTAATCTAAATGAGTCAGTTAAGAGAAAGATAAGATTTGCATGCAACATTATGGTTAACGCAAAAGGGATTAGAAAGGTTAAGATTCAAAGGAATGATGGTACAGAATCATTCATCTCGGATATGCTTTATGTCTCAAGTATGAGAAGCAATCTCACAAGTCTTTGACAGTTGCTTGAAAAAGGTTATACCATGAAGATGGAAGCATGTGCAATGAAGGTGTATAATAGCAAGGGTAGATTGATTTTAAAGGCACCATTGTCGGTGATAGCACTTTTATTTATTTTCAAGCACGGTCTAAGGTGAAAGCGCTGTCGCACACATATTTTTTTTTAGATTTAATTTTATTACTAAACATATGAGCATTGATTCATTAACAGCAGCAAGAGGAACCATTGGATACATGGCCCCTGAATTATTGTACAGAAATGTCGGCACAATATCTCACAAAGCTGATGTCTATAGCTTTGGAATGTTGTTGATGGAGATGGCGCAAGTAGGAGGAAGAACTTGAATGCATTGGCTGAGAAATCTTGCCAAATTTATTTCCCTTTTTGGATTTATGATCAGTTACATGATGGAAGTGAAGTAACAATTGAGAATGACACAAATCAAGAAATGAAATTCGCAAAGAAAATGATGATTGTGGCTTTATGGTGTATACAAACAAAACCCGAGGATCGTCCTTCAATGGATAAAGTGATTAAGATGCTTGAAGAAGATGGGGATTTGCAAATGCCTAATAAGCTTTACCTTTATCCACAAGATCTACCAGCTGAGGATTTTAGAGATAATAATACTAGAAGTTCGCGGTCTTCCTCTGATACATCAATAAATGATTTCATGGAAGCAACCTAA
Protein sequence

>MS.gene62979.t1

MVKVQSRVKNIAQKKKVQKAAEFKSKKVARDRADEEQFSYNEDESDADGVLLMATIKGENDDQWYLDTAARGTIGYMAPELLYRNVGTISHKADVYSFGMLLMEMAQLHDGSEVTIENDTNQEMKFAKKMMIVALWCIQTKPEDRPSMDKVIKMLEEDGDLQMPNKLYLYPQDLPAEDFRDNNTRSSRSSSDTSINDFMEAT