Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63046.t1 | AES97588.1 | 93.7 | 158 | 10 | 0 | 1 | 158 | 1 | 158 | 7.90E-81 | 309.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63046.t1 | G7K1J4 | 93.7 | 158 | 10 | 0 | 1 | 158 | 1 | 158 | 5.7e-81 | 309.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene63046.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63046.t1 | MTR_5g056450 | 93.671 | 158 | 10 | 0 | 1 | 158 | 1 | 158 | 3.29e-99 | 282 |
| MS.gene63046.t1 | MTR_1g041455 | 58.046 | 174 | 50 | 5 | 1 | 158 | 1 | 167 | 1.30e-58 | 180 |
| MS.gene63046.t1 | MTR_1g048880 | 47.368 | 171 | 59 | 7 | 1 | 158 | 1 | 153 | 1.07e-32 | 114 |
| MS.gene63046.t1 | MTR_1g075780 | 35.032 | 157 | 78 | 6 | 11 | 158 | 4 | 145 | 4.65e-14 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAATTGATTTCATTGATT+TGG | 0.224195 | 5.4:+44659086 | MS.gene63046:CDS |
| CATTTGAAGATTCTCCTTCT+AGG | 0.234818 | 5.4:-44659020 | None:intergenic |
| GGCCGAATCACCGTTGGTTT+TGG | 0.304502 | 5.4:-44658916 | None:intergenic |
| AAATCCAATGGATTAACATC+TGG | 0.363954 | 5.4:-44659129 | None:intergenic |
| TGCTTCGATTGGTAAGCATT+TGG | 0.404776 | 5.4:-44658997 | None:intergenic |
| AACCAAAACCAACGGTGATT+CGG | 0.418396 | 5.4:+44658914 | MS.gene63046:CDS |
| CTGGTGAGTTTAGTAATATT+AGG | 0.423811 | 5.4:+44658695 | MS.gene63046:CDS |
| TGAATAAAGCTTTCTGATTG+TGG | 0.433760 | 5.4:-44658821 | None:intergenic |
| TACCAATCGAAGCACCTAGA+AGG | 0.448280 | 5.4:+44659006 | MS.gene63046:CDS |
| CTCCTTCTAGGTGCTTCGAT+TGG | 0.488901 | 5.4:-44659008 | None:intergenic |
| GAAGGAGAATCTTCAAATGA+TGG | 0.491518 | 5.4:+44659024 | MS.gene63046:CDS |
| TAACCCAGATGTTAATCCAT+TGG | 0.496678 | 5.4:+44659125 | MS.gene63046:CDS |
| AATCCAATGGATTAACATCT+GGG | 0.500492 | 5.4:-44659128 | None:intergenic |
| ATCTTCAAATGATGGTACTA+AGG | 0.506473 | 5.4:+44659032 | MS.gene63046:CDS |
| GGCTGAGGCCGAATCACCGT+TGG | 0.513154 | 5.4:-44658922 | None:intergenic |
| AAAGGGTTTCTGCTTGGCTG+AGG | 0.532206 | 5.4:-44658937 | None:intergenic |
| TTCGATTGGTAAGCATTTGG+TGG | 0.535435 | 5.4:-44658994 | None:intergenic |
| CATGATGTGTCAAACTTGGT+AGG | 0.541015 | 5.4:+44658718 | MS.gene63046:CDS |
| TGCAACATCATCACCATGGC+TGG | 0.552771 | 5.4:+44658676 | None:intergenic |
| AATCAGAAAGCTTTATTCAA+CGG | 0.559288 | 5.4:+44658825 | MS.gene63046:CDS |
| TAGGCATGATGTGTCAAACT+TGG | 0.564079 | 5.4:+44658714 | MS.gene63046:CDS |
| AGCTTAAGTGATAAATCCAA+TGG | 0.575565 | 5.4:-44659141 | None:intergenic |
| CAATGTTTGCCACCACATGA+TGG | 0.576640 | 5.4:+44658880 | MS.gene63046:CDS |
| AACTCACATCGAGTCTCACA+TGG | 0.586787 | 5.4:+44658786 | MS.gene63046:CDS |
| ATTACTAAACTCACCAGCCA+TGG | 0.594645 | 5.4:-44658689 | None:intergenic |
| TCATGTGGTGGCAAACATTG+AGG | 0.597472 | 5.4:-44658877 | None:intergenic |
| GCATTTGCCGATAACCAACA+TGG | 0.630189 | 5.4:-44658966 | None:intergenic |
| ATGATGTGTCAAACTTGGTA+GGG | 0.645237 | 5.4:+44658719 | MS.gene63046:CDS |
| TGTGGTGGCAAACATTGAGG+AGG | 0.673338 | 5.4:-44658874 | None:intergenic |
| GTCTCACATGGAGTACGAAG+AGG | 0.675983 | 5.4:+44658798 | MS.gene63046:CDS |
| AAAATCTTGCCATCATGTGG+TGG | 0.685328 | 5.4:-44658889 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGAAATCAATTTTCTTGAT+AGG | - | chr5.4:44659081-44659100 | None:intergenic | 20.0% |
| !!! | GAAAATTGATTTCATTGATT+TGG | + | chr5.4:44659086-44659105 | MS.gene63046:CDS | 20.0% |
| !!! | AATCAGAAAGCTTTATTCAA+CGG | + | chr5.4:44658825-44658844 | MS.gene63046:CDS | 25.0% |
| AGCTTAAGTGATAAATCCAA+TGG | - | chr5.4:44659144-44659163 | None:intergenic | 30.0% | |
| ATCTTCAAATGATGGTACTA+AGG | + | chr5.4:44659032-44659051 | MS.gene63046:CDS | 30.0% | |
| CTGGTGAGTTTAGTAATATT+AGG | + | chr5.4:44658695-44658714 | MS.gene63046:CDS | 30.0% | |
| TGAATAAAGCTTTCTGATTG+TGG | - | chr5.4:44658824-44658843 | None:intergenic | 30.0% | |
| TTTGAGTGTTTGTGAAAAAG+CGG | - | chr5.4:44658759-44658778 | None:intergenic | 30.0% | |
| !! | AAATCCAATGGATTAACATC+TGG | - | chr5.4:44659132-44659151 | None:intergenic | 30.0% |
| !! | AATCCAATGGATTAACATCT+GGG | - | chr5.4:44659131-44659150 | None:intergenic | 30.0% |
| !! | GATTTTTCAACCAAAACCAA+CGG | + | chr5.4:44658906-44658925 | MS.gene63046:CDS | 30.0% |
| ATGATGTGTCAAACTTGGTA+GGG | + | chr5.4:44658719-44658738 | MS.gene63046:CDS | 35.0% | |
| CATTTGAAGATTCTCCTTCT+AGG | - | chr5.4:44659023-44659042 | None:intergenic | 35.0% | |
| GAAGGAGAATCTTCAAATGA+TGG | + | chr5.4:44659024-44659043 | MS.gene63046:CDS | 35.0% | |
| TAACCCAGATGTTAATCCAT+TGG | + | chr5.4:44659125-44659144 | MS.gene63046:CDS | 35.0% | |
| TGAAAAATCTTGCCATCATG+TGG | - | chr5.4:44658895-44658914 | None:intergenic | 35.0% | |
| ! | GAGGAGGAAATTGTCTTTTA+GGG | - | chr5.4:44658861-44658880 | None:intergenic | 35.0% |
| ! | TGAGGAGGAAATTGTCTTTT+AGG | - | chr5.4:44658862-44658881 | None:intergenic | 35.0% |
| AAAATCTTGCCATCATGTGG+TGG | - | chr5.4:44658892-44658911 | None:intergenic | 40.0% | |
| AACCAAAACCAACGGTGATT+CGG | + | chr5.4:44658914-44658933 | MS.gene63046:CDS | 40.0% | |
| AACCAACATGGCCGAAAAAA+GGG | - | chr5.4:44658957-44658976 | None:intergenic | 40.0% | |
| CATGATGTGTCAAACTTGGT+AGG | + | chr5.4:44658718-44658737 | MS.gene63046:CDS | 40.0% | |
| TAACCAACATGGCCGAAAAA+AGG | - | chr5.4:44658958-44658977 | None:intergenic | 40.0% | |
| TAGGCATGATGTGTCAAACT+TGG | + | chr5.4:44658714-44658733 | MS.gene63046:CDS | 40.0% | |
| TGCTTCGATTGGTAAGCATT+TGG | - | chr5.4:44659000-44659019 | None:intergenic | 40.0% | |
| TTCGATTGGTAAGCATTTGG+TGG | - | chr5.4:44658997-44659016 | None:intergenic | 40.0% | |
| !! | TTTTCGGCCATGTTGGTTAT+CGG | + | chr5.4:44658959-44658978 | MS.gene63046:CDS | 40.0% |
| !!! | CCAAGCAGAAACCCTTTTTT+CGG | + | chr5.4:44658943-44658962 | MS.gene63046:CDS | 40.0% |
| AACTCACATCGAGTCTCACA+TGG | + | chr5.4:44658786-44658805 | MS.gene63046:CDS | 45.0% | |
| CAATGTTTGCCACCACATGA+TGG | + | chr5.4:44658880-44658899 | MS.gene63046:CDS | 45.0% | |
| CCGAAAAAAGGGTTTCTGCT+TGG | - | chr5.4:44658946-44658965 | None:intergenic | 45.0% | |
| GCATTTGCCGATAACCAACA+TGG | - | chr5.4:44658969-44658988 | None:intergenic | 45.0% | |
| TCATGTGGTGGCAAACATTG+AGG | - | chr5.4:44658880-44658899 | None:intergenic | 45.0% | |
| ! | TACCAATCGAAGCACCTAGA+AGG | + | chr5.4:44659006-44659025 | MS.gene63046:CDS | 45.0% |
| !!! | AACCCTTTTTTCGGCCATGT+TGG | + | chr5.4:44658952-44658971 | MS.gene63046:CDS | 45.0% |
| AAAGGGTTTCTGCTTGGCTG+AGG | - | chr5.4:44658940-44658959 | None:intergenic | 50.0% | |
| CTCCTTCTAGGTGCTTCGAT+TGG | - | chr5.4:44659011-44659030 | None:intergenic | 50.0% | |
| GTCTCACATGGAGTACGAAG+AGG | + | chr5.4:44658798-44658817 | MS.gene63046:CDS | 50.0% | |
| TGTGGTGGCAAACATTGAGG+AGG | - | chr5.4:44658877-44658896 | None:intergenic | 50.0% | |
| !!! | GGCCGAATCACCGTTGGTTT+TGG | - | chr5.4:44658919-44658938 | None:intergenic | 55.0% |
| GGCTGAGGCCGAATCACCGT+TGG | - | chr5.4:44658925-44658944 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 44658691 | 44659167 | 44658691 | ID=MS.gene63046 |
| chr5.4 | mRNA | 44658691 | 44659167 | 44658691 | ID=MS.gene63046.t1;Parent=MS.gene63046 |
| chr5.4 | exon | 44658691 | 44659167 | 44658691 | ID=MS.gene63046.t1.exon1;Parent=MS.gene63046.t1 |
| chr5.4 | CDS | 44658691 | 44659167 | 44658691 | ID=cds.MS.gene63046.t1;Parent=MS.gene63046.t1 |
| Gene Sequence |
| Protein sequence |
