AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63127

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 121 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTATGTGGAGGCGGATTT+TGG 0.148513 8.1:+43271789 None:intergenic
AGATTTGTTGCACGAGTGTT+TGG 0.301888 8.1:-43271442 MS.gene63127:CDS
TTCCTCCAGCGGCATTTCTC+AGG 0.312507 8.1:-43271333 MS.gene63127:CDS
CCTGATTGCTGCCGAGATAC+TGG 0.331416 8.1:-43272032 MS.gene63127:CDS
TTCGGGATGAGGAGGAGAAT+TGG 0.336432 8.1:+43271853 None:intergenic
ACTCGTGCAACAAATCTTCT+TGG 0.342234 8.1:+43271448 None:intergenic
GGTGGGAATGTGGCTAATAA+TGG 0.350488 8.1:-43271638 MS.gene63127:CDS
CTATAGGCTAAGGAGTTTGC+TGG 0.354712 8.1:-43272191 MS.gene63127:CDS
ACTCCTTAGCCTATAGAAAA+AGG 0.359699 8.1:+43272198 None:intergenic
TAAGACACACTTACCTTGTC+TGG 0.362065 8.1:+43273017 None:intergenic
TTCAACTCCTGTGGCAGAAC+CGG 0.369949 8.1:-43271900 MS.gene63127:CDS
TCCCTAGCATACATAACATA+TGG 0.379144 8.1:+43271232 None:intergenic
GACACAAGAGGTTCGAGGTT+TGG 0.386987 8.1:-43272250 MS.gene63127:CDS
TCATGGCCGCGCTTACATTC+AGG 0.392786 8.1:-43273061 MS.gene63127:CDS
TGGCAGTTGGGAAAAGTGTT+AGG 0.395161 8.1:-43271276 MS.gene63127:CDS
GGCATTGTGTGGTTGTTGAT+TGG 0.403874 8.1:+43271820 None:intergenic
TGATTGGCATCATGAACTTC+GGG 0.411728 8.1:+43271836 None:intergenic
CGGAGAACCGGCTGGCAGTT+GGG 0.421662 8.1:-43271288 MS.gene63127:CDS
TGATGCTGCTGCTGTTGCCA+TGG 0.428743 8.1:-43271687 MS.gene63127:CDS
TCCTCCAGCGGCATTTCTCA+GGG 0.443998 8.1:-43271332 MS.gene63127:CDS
CGGACAGGGGCTGTTCTACA+TGG 0.447788 8.1:-43272122 MS.gene63127:CDS
TCGGGATGAGGAGGAGAATT+GGG 0.451241 8.1:+43271854 None:intergenic
GACGATGATTCCATCGATCT+TGG 0.451452 8.1:-43271953 MS.gene63127:CDS
GATTTGTTGCACGAGTGTTT+GGG 0.452697 8.1:-43271441 MS.gene63127:CDS
AGAGATTTGTTACGGAGAAC+CGG 0.452877 8.1:-43271300 MS.gene63127:CDS
GTGGGAATGTGGCTAATAAT+GGG 0.456886 8.1:-43271637 MS.gene63127:CDS
CCAGTATCTCGGCAGCAATC+AGG 0.457630 8.1:+43272032 None:intergenic
GAGCACTTCCACCAGCCGTT+AGG 0.466631 8.1:-43272169 MS.gene63127:CDS
CGAGATACTGGATCCCTTGG+AGG 0.468114 8.1:-43272020 MS.gene63127:CDS
ATCGTCGTCGTTGTCTTCAT+CGG 0.472155 8.1:+43271970 None:intergenic
ACATCAACATCCGCTGGATC+CGG 0.472816 8.1:+43271881 None:intergenic
TTGATTGGCATCATGAACTT+CGG 0.473733 8.1:+43271835 None:intergenic
CAGCGGCATTTCTCAGGGGC+GGG 0.478818 8.1:-43271327 MS.gene63127:CDS
ATGAACTACGACACAGAGTC+TGG 0.481562 8.1:-43272279 MS.gene63127:CDS
ACAGGAGTTGAAGGAGGTGC+AGG 0.491094 8.1:+43271911 None:intergenic
CATGGTGAAGACTAGCAATC+TGG 0.491937 8.1:-43271579 MS.gene63127:intron
ACGGAGAACCGGCTGGCAGT+TGG 0.492128 8.1:-43271289 MS.gene63127:CDS
GCTGGATCCGGTTCTGCCAC+AGG 0.493035 8.1:+43271893 None:intergenic
GATGCTGCTGCTGTTGCCAT+GGG 0.497052 8.1:-43271686 MS.gene63127:CDS
TGGGCTTTGACTTTCCGTGC+AGG 0.505024 8.1:-43271381 MS.gene63127:CDS
CGGCATTTCTCAGGGGCGGG+TGG 0.507786 8.1:-43271324 MS.gene63127:CDS
CCCTAGCATACATAACATAT+GGG 0.511261 8.1:+43271233 None:intergenic
GCATCATGAACTTCGGGATG+AGG 0.512150 8.1:+43271842 None:intergenic
GGTTCTGCCACAGGAGTTGA+AGG 0.512252 8.1:+43271902 None:intergenic
TCATAGGAAGTGAGGGGACG+CGG 0.513245 8.1:-43272222 MS.gene63127:CDS
AACGATGAAGATGTGGAAGA+TGG 0.513612 8.1:-43271737 MS.gene63127:CDS
CCAGCGGCATTTCTCAGGGG+CGG 0.516500 8.1:-43271328 MS.gene63127:CDS
GCAAAATCAAAGAAATTATG+TGG 0.521917 8.1:+43271776 None:intergenic
TGCCGAGATACTGGATCCCT+TGG 0.522650 8.1:-43272023 MS.gene63127:CDS
TCATACTTCAACAACCACTT+TGG 0.527976 8.1:-43272304 MS.gene63127:CDS
ACCCATATGTTATGTATGCT+AGG 0.529159 8.1:-43271234 MS.gene63127:CDS
GTATGCTAGGGAAGCTCGGA+TGG 0.537149 8.1:-43271221 MS.gene63127:CDS
AAGTGGTTGTTGAAGTATGA+TGG 0.538484 8.1:+43272307 None:intergenic
AACATCAATGCCAAGATCGA+TGG 0.539611 8.1:+43271943 None:intergenic
GCGTCTAAGTTACAACGGAC+AGG 0.542298 8.1:-43272137 MS.gene63127:CDS
CCGCCCCTGAGAAATGCCGC+TGG 0.546065 8.1:+43271328 None:intergenic
CATCCTACTTACAGATGCCA+TGG 0.546268 8.1:-43271401 MS.gene63127:CDS
ATTTGTTACGGAGAACCGGC+TGG 0.553165 8.1:-43271296 MS.gene63127:CDS
TCAAAGAAATTATGTGGAGG+CGG 0.554584 8.1:+43271782 None:intergenic
ATCTTCATCGTTGATATCCA+TGG 0.554841 8.1:+43271748 None:intergenic
CCCATATGTTATGTATGCTA+GGG 0.556719 8.1:-43271233 MS.gene63127:CDS
ACTCGACATGACCGAGCTAA+CGG 0.557804 8.1:-43272089 MS.gene63127:CDS
CTCCAAGGGATCCAGTATCT+CGG 0.558445 8.1:+43272021 None:intergenic
TAGAAGACACAAGAGGTTCG+AGG 0.561323 8.1:-43272255 MS.gene63127:CDS
TGTGGCTAATAATGGGAGAG+TGG 0.562385 8.1:-43271630 MS.gene63127:CDS
TCCTTCCACTTGATCCTGCA+CGG 0.565208 8.1:+43271367 None:intergenic
TGCACCTCCTTCAACTCCTG+TGG 0.569911 8.1:-43271909 MS.gene63127:CDS
GGTGCAACATCAACATCCGC+TGG 0.570592 8.1:+43271875 None:intergenic
GTATAATTCATACTCCTCCA+AGG 0.570762 8.1:+43272006 None:intergenic
TTATGTATGCTAGGGAAGCT+CGG 0.572454 8.1:-43271225 MS.gene63127:CDS
GGCATCTGTAAGTAGGATGG+TGG 0.575659 8.1:+43271405 None:intergenic
CGTCTAAGTTACAACGGACA+GGG 0.576116 8.1:-43272136 MS.gene63127:CDS
GGTGAAAACATGGGTGCAGG+TGG 0.578164 8.1:-43271656 MS.gene63127:CDS
ACTTTCCGTGCAGGATCAAG+TGG 0.580280 8.1:-43271372 MS.gene63127:CDS
CATGGGTGCAGGTGGGAATG+TGG 0.582106 8.1:-43271648 MS.gene63127:CDS
CCTCTCACGAACCGTTAGCT+CGG 0.586517 8.1:+43272078 None:intergenic
CGGGTGGAAGAGATTTGTTA+CGG 0.590170 8.1:-43271308 MS.gene63127:CDS
GAAATGCCGCTGGAGGAAGA+TGG 0.590786 8.1:+43271338 None:intergenic
CCGAGCTAACGGTTCGTGAG+AGG 0.592918 8.1:-43272078 MS.gene63127:CDS
CCTCCAGCGGCATTTCTCAG+GGG 0.594266 8.1:-43271331 MS.gene63127:CDS
GGAGTTGAAGGAGGTGCAGG+AGG 0.595751 8.1:+43271914 None:intergenic
GGGTAACAATGGTGAAAACA+TGG 0.597431 8.1:-43271666 MS.gene63127:CDS
TGTGGCAGAACCGGATCCAG+CGG 0.598738 8.1:-43271891 MS.gene63127:CDS
CCCCTGAGAAATGCCGCTGG+AGG 0.603022 8.1:+43271331 None:intergenic
TCATGAACTTCGGGATGAGG+AGG 0.603718 8.1:+43271845 None:intergenic
CATGGCATCTGTAAGTAGGA+TGG 0.604155 8.1:+43271402 None:intergenic
GTGCAACAAATCTTCTTGGT+AGG 0.604465 8.1:+43271452 None:intergenic
TCGGCAGCAATCAGGAAAGG+AGG 0.606699 8.1:+43272040 None:intergenic
GCAGTATGAGACCTAACGGC+TGG 0.608111 8.1:+43272158 None:intergenic
AGCCCATGGCATCTGTAAGT+AGG 0.608677 8.1:+43271398 None:intergenic
CACAACAATCGATCCAGACA+AGG 0.610416 8.1:-43273030 MS.gene63127:intron
ATCTCGGCAGCAATCAGGAA+AGG 0.610893 8.1:+43272037 None:intergenic
ATCCTACTTACAGATGCCAT+GGG 0.611432 8.1:-43271400 MS.gene63127:CDS
TGTCAGACTTGCAGAATACA+TGG 0.612787 8.1:-43271597 MS.gene63127:CDS
GGATATCAACGATGAAGATG+TGG 0.616665 8.1:-43271744 MS.gene63127:CDS
TATAATTCATACTCCTCCAA+GGG 0.616989 8.1:+43272007 None:intergenic
GTATGAGACCTAACGGCTGG+TGG 0.617542 8.1:+43272161 None:intergenic
CGGCAGCAATCAGGAAAGGA+GGG 0.618638 8.1:+43272041 None:intergenic
AATGGTGAAAACATGGGTGC+AGG 0.619307 8.1:-43271659 MS.gene63127:CDS
CAGAGGCCATCTTCCTCCAG+CGG 0.626292 8.1:-43271344 MS.gene63127:CDS
GCATCTGTAAGTAGGATGGT+GGG 0.632206 8.1:+43271406 None:intergenic
GCACGGAAAGTCAAAGCCCA+TGG 0.634938 8.1:+43271384 None:intergenic
TCCGTGCAGGATCAAGTGGA+AGG 0.637315 8.1:-43271368 MS.gene63127:CDS
TGGGCATCATCGGCATTGTG+TGG 0.641687 8.1:+43271809 None:intergenic
GTCGTAGTTCATCACCAAAG+TGG 0.648562 8.1:+43272290 None:intergenic
AGACGCAGTATGAGACCTAA+CGG 0.653534 8.1:+43272154 None:intergenic
GCTGTTGCCATGGGTAACAA+TGG 0.655928 8.1:-43271677 MS.gene63127:CDS
GCAGCATCAAAGAAGTTATG+CGG 0.656305 8.1:+43271701 None:intergenic
GGACAGGGGCTGTTCTACAT+GGG 0.657468 8.1:-43272121 MS.gene63127:CDS
GAACTTCCTGAATGTAAGCG+CGG 0.670661 8.1:+43273055 None:intergenic
GTGAAAACATGGGTGCAGGT+GGG 0.671071 8.1:-43271655 MS.gene63127:CDS
GTTGTCTTCATCGGATGACG+AGG 0.675992 8.1:+43271979 None:intergenic
GCATCAAAGAAGTTATGCGG+AGG 0.686757 8.1:+43271704 None:intergenic
AGGATCAAGTGGAAGGACAG+AGG 0.686964 8.1:-43271361 MS.gene63127:CDS
GTCTGGTTAGAAGACACAAG+AGG 0.687596 8.1:-43272262 MS.gene63127:CDS
ATACTGCGTCTAAGTTACAA+CGG 0.691908 8.1:-43272142 MS.gene63127:CDS
AAATCAAAGAAATTATGTGG+AGG 0.696121 8.1:+43271779 None:intergenic
TCTGCCACAGGAGTTGAAGG+AGG 0.696157 8.1:+43271905 None:intergenic
TCTGTAAGTAGGATGGTGGG+CGG 0.704271 8.1:+43271409 None:intergenic
GTCTAAGTTACAACGGACAG+GGG 0.730281 8.1:-43272135 MS.gene63127:CDS
GGTAACAATGGTGAAAACAT+GGG 0.742892 8.1:-43271665 MS.gene63127:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATTTAAAAAAGAACAC+AGG - chr8.1:43271848-43271867 MS.gene63127:CDS 15.0%
!!! AAATGATAACAATTTAAAAC+AGG + chr8.1:43272790-43272809 None:intergenic 15.0%
!!! AATTACTGTTATATAGTTTT+TGG - chr8.1:43272749-43272768 MS.gene63127:intron 15.0%
! AAATCAAAGAAATTATGTGG+AGG + chr8.1:43272496-43272515 None:intergenic 25.0%
! AGTACACAAAATCTTAGATA+CGG + chr8.1:43271508-43271527 None:intergenic 25.0%
! GCAAAATCAAAGAAATTATG+TGG + chr8.1:43272499-43272518 None:intergenic 25.0%
!!! CATTTGTTGTTACATGTTTA+AGG - chr8.1:43271395-43271414 MS.gene63127:CDS 25.0%
AAATATATTGCAGAGTGACA+AGG + chr8.1:43271591-43271610 None:intergenic 30.0%
AGTACACATTATCGTAGATA+CGG + chr8.1:43271695-43271714 None:intergenic 30.0%
TATAATTCATACTCCTCCAA+GGG + chr8.1:43272268-43272287 None:intergenic 30.0%
TTGTTACTAAACTTTGAGTG+TGG - chr8.1:43271292-43271311 MS.gene63127:CDS 30.0%
AAAATCTACCGGATACCAAA+AGG + chr8.1:43271366-43271385 None:intergenic 35.0%
AAATCTACCGGATACCAAAA+GGG + chr8.1:43271365-43271384 None:intergenic 35.0%
AAGTGGTTGTTGAAGTATGA+TGG + chr8.1:43271968-43271987 None:intergenic 35.0%
ACCCATATGTTATGTATGCT+AGG - chr8.1:43273038-43273057 MS.gene63127:CDS 35.0%
ACTCCTTAGCCTATAGAAAA+AGG + chr8.1:43272077-43272096 None:intergenic 35.0%
ATACTGCGTCTAAGTTACAA+CGG - chr8.1:43272130-43272149 MS.gene63127:CDS 35.0%
ATCTTCATCGTTGATATCCA+TGG + chr8.1:43272527-43272546 None:intergenic 35.0%
ATTACAGAGTGACAACAAAC+AGG + chr8.1:43271552-43271571 None:intergenic 35.0%
ATTACAGAGTGACAACAAAC+TGG + chr8.1:43271552-43271571 None:intergenic 35.0%
CCCATATGTTATGTATGCTA+GGG - chr8.1:43273039-43273058 MS.gene63127:CDS 35.0%
CCCTAGCATACATAACATAT+GGG + chr8.1:43273042-43273061 None:intergenic 35.0%
GGTAACAATGGTGAAAACAT+GGG - chr8.1:43272607-43272626 MS.gene63127:intron 35.0%
GTATAATTCATACTCCTCCA+AGG + chr8.1:43272269-43272288 None:intergenic 35.0%
TCAAAGAAATTATGTGGAGG+CGG + chr8.1:43272493-43272512 None:intergenic 35.0%
TCATACTTCAACAACCACTT+TGG - chr8.1:43271968-43271987 MS.gene63127:CDS 35.0%
TCCCTAGCATACATAACATA+TGG + chr8.1:43273043-43273062 None:intergenic 35.0%
TTACAGAGTGACAACAAACT+GGG + chr8.1:43271738-43271757 None:intergenic 35.0%
TTCTTGTGTGTGCATAATAG+TGG + chr8.1:43271484-43271503 None:intergenic 35.0%
TTGATTGGCATCATGAACTT+CGG + chr8.1:43272440-43272459 None:intergenic 35.0%
TTTACAGAGTGACATCAAAC+TGG + chr8.1:43271777-43271796 None:intergenic 35.0%
! GAAGTTGTCACTTGTAATGT+AGG - chr8.1:43271928-43271947 MS.gene63127:CDS 35.0%
! TTATTTTCCTGTCTCCCTTT+TGG - chr8.1:43271348-43271367 MS.gene63127:CDS 35.0%
AACATCAATGCCAAGATCGA+TGG + chr8.1:43272332-43272351 None:intergenic 40.0%
AACCAACAACAAGGCAAACA+TGG + chr8.1:43271617-43271636 None:intergenic 40.0%
AACGATGAAGATGTGGAAGA+TGG - chr8.1:43272535-43272554 MS.gene63127:intron 40.0%
ACTCGTGCAACAAATCTTCT+TGG + chr8.1:43272827-43272846 None:intergenic 40.0%
ATCCTACTTACAGATGCCAT+GGG - chr8.1:43272872-43272891 MS.gene63127:intron 40.0%
ATGTTTGCCTTCTTGTTGGT+TGG - chr8.1:43271427-43271446 MS.gene63127:CDS 40.0%
GACTATGTTTGCCTTCTTGT+TGG - chr8.1:43271423-43271442 MS.gene63127:CDS 40.0%
GCAGCATCAAAGAAGTTATG+CGG + chr8.1:43272574-43272593 None:intergenic 40.0%
GGATATCAACGATGAAGATG+TGG - chr8.1:43272528-43272547 MS.gene63127:intron 40.0%
GGGTAACAATGGTGAAAACA+TGG - chr8.1:43272606-43272625 MS.gene63127:intron 40.0%
GTCACTTGTAATGTAGGATG+AGG - chr8.1:43271934-43271953 MS.gene63127:CDS 40.0%
GTGCAACAAATCTTCTTGGT+AGG + chr8.1:43272823-43272842 None:intergenic 40.0%
GTGGGAATGTGGCTAATAAT+GGG - chr8.1:43272635-43272654 MS.gene63127:intron 40.0%
TAAGACACACTTACCTTGTC+TGG + chr8.1:43271258-43271277 None:intergenic 40.0%
TCATCATCCAACCAACAACA+AGG + chr8.1:43271626-43271645 None:intergenic 40.0%
TCATCATCCAACCAACAAGA+AGG + chr8.1:43271437-43271456 None:intergenic 40.0%
TGAAGCCATGCAAAATCTAC+CGG + chr8.1:43271377-43271396 None:intergenic 40.0%
TGATTGGCATCATGAACTTC+GGG + chr8.1:43272439-43272458 None:intergenic 40.0%
TGTCAGACTTGCAGAATACA+TGG - chr8.1:43272675-43272694 MS.gene63127:intron 40.0%
TTATGTATGCTAGGGAAGCT+CGG - chr8.1:43273047-43273066 MS.gene63127:CDS 40.0%
! GGACCTTTTTCTATAGGCTA+AGG - chr8.1:43272071-43272090 MS.gene63127:CDS 40.0%
! GGTGTTTTCATAGGAAGTGA+GGG - chr8.1:43272043-43272062 MS.gene63127:CDS 40.0%
! GTGTTTTCATAGGAAGTGAG+GGG - chr8.1:43272044-43272063 MS.gene63127:CDS 40.0%
! GTTTTCACCATTGTTACCCA+TGG + chr8.1:43272605-43272624 None:intergenic 40.0%
! TGGTGTTTTCATAGGAAGTG+AGG - chr8.1:43272042-43272061 MS.gene63127:CDS 40.0%
!! AATTATGTGGAGGCGGATTT+TGG + chr8.1:43272486-43272505 None:intergenic 40.0%
!! AGAGATTTGTTACGGAGAAC+CGG - chr8.1:43272972-43272991 MS.gene63127:intron 40.0%
!! AGATTTGTTGCACGAGTGTT+TGG - chr8.1:43272830-43272849 MS.gene63127:intron 40.0%
!! ATGTTTGCCTTGTTGTTGGT+TGG - chr8.1:43271616-43271635 MS.gene63127:CDS 40.0%
!! ATTATGTGGAGGCGGATTTT+GGG + chr8.1:43272485-43272504 None:intergenic 40.0%
!! GATTTGTTGCACGAGTGTTT+GGG - chr8.1:43272831-43272850 MS.gene63127:intron 40.0%
!!! TCGAGGTTTGGTGTTTTCAT+AGG - chr8.1:43272034-43272053 MS.gene63127:CDS 40.0%
AATGGTGAAAACATGGGTGC+AGG - chr8.1:43272613-43272632 MS.gene63127:intron 45.0%
AGACGCAGTATGAGACCTAA+CGG + chr8.1:43272121-43272140 None:intergenic 45.0%
ATCGTCGTCGTTGTCTTCAT+CGG + chr8.1:43272305-43272324 None:intergenic 45.0%
ATGAACTACGACACAGAGTC+TGG - chr8.1:43271993-43272012 MS.gene63127:CDS 45.0%
CACAACAATCGATCCAGACA+AGG - chr8.1:43271242-43271261 MS.gene63127:CDS 45.0%
CATCCTACTTACAGATGCCA+TGG - chr8.1:43272871-43272890 MS.gene63127:intron 45.0%
CATGGCATCTGTAAGTAGGA+TGG + chr8.1:43272873-43272892 None:intergenic 45.0%
CATGGTGAAGACTAGCAATC+TGG - chr8.1:43272693-43272712 MS.gene63127:intron 45.0%
CGTCTAAGTTACAACGGACA+GGG - chr8.1:43272136-43272155 MS.gene63127:CDS 45.0%
CTATAGGCTAAGGAGTTTGC+TGG - chr8.1:43272081-43272100 MS.gene63127:CDS 45.0%
GAACTTCCTGAATGTAAGCG+CGG + chr8.1:43271220-43271239 None:intergenic 45.0%
GACGATGATTCCATCGATCT+TGG - chr8.1:43272319-43272338 MS.gene63127:CDS 45.0%
GCATCAAAGAAGTTATGCGG+AGG + chr8.1:43272571-43272590 None:intergenic 45.0%
GCATCTGTAAGTAGGATGGT+GGG + chr8.1:43272869-43272888 None:intergenic 45.0%
GGCATTGTGTGGTTGTTGAT+TGG + chr8.1:43272455-43272474 None:intergenic 45.0%
GGTGGGAATGTGGCTAATAA+TGG - chr8.1:43272634-43272653 MS.gene63127:intron 45.0%
GTCGTAGTTCATCACCAAAG+TGG + chr8.1:43271985-43272004 None:intergenic 45.0%
GTCTAAGTTACAACGGACAG+GGG - chr8.1:43272137-43272156 MS.gene63127:CDS 45.0%
GTCTGGTTAGAAGACACAAG+AGG - chr8.1:43272010-43272029 MS.gene63127:CDS 45.0%
GTGAAGACTAGCAATCTGGT+AGG - chr8.1:43272697-43272716 MS.gene63127:intron 45.0%
TAGAAGACACAAGAGGTTCG+AGG - chr8.1:43272017-43272036 MS.gene63127:CDS 45.0%
! GACCATGTTTGCCTTGTTGT+TGG - chr8.1:43271612-43271631 MS.gene63127:CDS 45.0%
! GGTATCCGGTAGATTTTGCA+TGG - chr8.1:43271369-43271388 MS.gene63127:CDS 45.0%
! TGTGGCTAATAATGGGAGAG+TGG - chr8.1:43272642-43272661 MS.gene63127:intron 45.0%
!! CGGGTGGAAGAGATTTGTTA+CGG - chr8.1:43272964-43272983 MS.gene63127:intron 45.0%
!! CTTTGATTTTGCTGCTGCCA+TGG - chr8.1:43272507-43272526 MS.gene63127:intron 45.0%
!! TGGCAGTTGGGAAAAGTGTT+AGG - chr8.1:43272996-43273015 MS.gene63127:intron 45.0%
ACATCAACATCCGCTGGATC+CGG + chr8.1:43272394-43272413 None:intergenic 50.0%
ACTCGACATGACCGAGCTAA+CGG - chr8.1:43272183-43272202 MS.gene63127:CDS 50.0%
ACTTTCCGTGCAGGATCAAG+TGG - chr8.1:43272900-43272919 MS.gene63127:intron 50.0%
AGCCCATGGCATCTGTAAGT+AGG + chr8.1:43272877-43272896 None:intergenic 50.0%
AGGATCAAGTGGAAGGACAG+AGG - chr8.1:43272911-43272930 MS.gene63127:intron 50.0%
ATCTCGGCAGCAATCAGGAA+AGG + chr8.1:43272238-43272257 None:intergenic 50.0%
CTCCAAGGGATCCAGTATCT+CGG + chr8.1:43272254-43272273 None:intergenic 50.0%
GACACAAGAGGTTCGAGGTT+TGG - chr8.1:43272022-43272041 MS.gene63127:CDS 50.0%
GCATCATGAACTTCGGGATG+AGG + chr8.1:43272433-43272452 None:intergenic 50.0%
GCGTCTAAGTTACAACGGAC+AGG - chr8.1:43272135-43272154 MS.gene63127:CDS 50.0%
GCTGTTGCCATGGGTAACAA+TGG - chr8.1:43272595-43272614 MS.gene63127:intron 50.0%
GGCATCTGTAAGTAGGATGG+TGG + chr8.1:43272870-43272889 None:intergenic 50.0%
GTGAAAACATGGGTGCAGGT+GGG - chr8.1:43272617-43272636 MS.gene63127:intron 50.0%
GTTGTCTTCATCGGATGACG+AGG + chr8.1:43272296-43272315 None:intergenic 50.0%
TCATGAACTTCGGGATGAGG+AGG + chr8.1:43272430-43272449 None:intergenic 50.0%
TCCTTCCACTTGATCCTGCA+CGG + chr8.1:43272908-43272927 None:intergenic 50.0%
TCGGGATGAGGAGGAGAATT+GGG + chr8.1:43272421-43272440 None:intergenic 50.0%
TTCAACTCCTGTGGCAGAAC+CGG - chr8.1:43272372-43272391 MS.gene63127:intron 50.0%
TTCGGGATGAGGAGGAGAAT+TGG + chr8.1:43272422-43272441 None:intergenic 50.0%
! ATTTGTTACGGAGAACCGGC+TGG - chr8.1:43272976-43272995 MS.gene63127:intron 50.0%
! CCTGTCTCCCTTTTGGTATC+CGG - chr8.1:43271355-43271374 MS.gene63127:CDS 50.0%
! GGACGCGGACCTTTTTCTAT+AGG - chr8.1:43272065-43272084 MS.gene63127:CDS 50.0%
!! AGGCGGATTTTGGGCATCAT+CGG + chr8.1:43272476-43272495 None:intergenic 50.0%
!! TCTGTAAGTAGGATGGTGGG+CGG + chr8.1:43272866-43272885 None:intergenic 50.0%
CCAGTATCTCGGCAGCAATC+AGG + chr8.1:43272243-43272262 None:intergenic 55.0%
CCGGATACCAAAAGGGAGAC+AGG + chr8.1:43271358-43271377 None:intergenic 55.0%
CCTCTCACGAACCGTTAGCT+CGG + chr8.1:43272197-43272216 None:intergenic 55.0%
CCTGATTGCTGCCGAGATAC+TGG - chr8.1:43272240-43272259 MS.gene63127:CDS 55.0%
CGAGATACTGGATCCCTTGG+AGG - chr8.1:43272252-43272271 MS.gene63127:CDS 55.0%
CGGCAGCAATCAGGAAAGGA+GGG + chr8.1:43272234-43272253 None:intergenic 55.0%
GAAATGCCGCTGGAGGAAGA+TGG + chr8.1:43272937-43272956 None:intergenic 55.0%
GATGCTGCTGCTGTTGCCAT+GGG - chr8.1:43272586-43272605 MS.gene63127:intron 55.0%
GCACGGAAAGTCAAAGCCCA+TGG + chr8.1:43272891-43272910 None:intergenic 55.0%
GCAGTATGAGACCTAACGGC+TGG + chr8.1:43272117-43272136 None:intergenic 55.0%
GGACAGGGGCTGTTCTACAT+GGG - chr8.1:43272151-43272170 MS.gene63127:CDS 55.0%
GGTGAAAACATGGGTGCAGG+TGG - chr8.1:43272616-43272635 MS.gene63127:intron 55.0%
GGTGCAACATCAACATCCGC+TGG + chr8.1:43272400-43272419 None:intergenic 55.0%
GGTTCTGCCACAGGAGTTGA+AGG + chr8.1:43272373-43272392 None:intergenic 55.0%
GTATGAGACCTAACGGCTGG+TGG + chr8.1:43272114-43272133 None:intergenic 55.0%
GTATGCTAGGGAAGCTCGGA+TGG - chr8.1:43273051-43273070 MS.gene63127:CDS 55.0%
TCATAGGAAGTGAGGGGACG+CGG - chr8.1:43272050-43272069 MS.gene63127:CDS 55.0%
TCCGTGCAGGATCAAGTGGA+AGG - chr8.1:43272904-43272923 MS.gene63127:intron 55.0%
TCCTCCAGCGGCATTTCTCA+GGG - chr8.1:43272940-43272959 MS.gene63127:intron 55.0%
TCGGCAGCAATCAGGAAAGG+AGG + chr8.1:43272235-43272254 None:intergenic 55.0%
TCTGCCACAGGAGTTGAAGG+AGG + chr8.1:43272370-43272389 None:intergenic 55.0%
TGATGCTGCTGCTGTTGCCA+TGG - chr8.1:43272585-43272604 MS.gene63127:intron 55.0%
TGCACCTCCTTCAACTCCTG+TGG - chr8.1:43272363-43272382 MS.gene63127:intron 55.0%
TGCCGAGATACTGGATCCCT+TGG - chr8.1:43272249-43272268 MS.gene63127:CDS 55.0%
TGGGCATCATCGGCATTGTG+TGG + chr8.1:43272466-43272485 None:intergenic 55.0%
TTCCTCCAGCGGCATTTCTC+AGG - chr8.1:43272939-43272958 MS.gene63127:intron 55.0%
! ACAGGAGTTGAAGGAGGTGC+AGG + chr8.1:43272364-43272383 None:intergenic 55.0%
! CACTTTTCCCAACTGCCAGC+CGG + chr8.1:43272994-43273013 None:intergenic 55.0%
! TGGGCTTTGACTTTCCGTGC+AGG - chr8.1:43272891-43272910 MS.gene63127:intron 55.0%
CAGAGGCCATCTTCCTCCAG+CGG - chr8.1:43272928-43272947 MS.gene63127:intron 60.0%
CATGGGTGCAGGTGGGAATG+TGG - chr8.1:43272624-43272643 MS.gene63127:intron 60.0%
CCGAGCTAACGGTTCGTGAG+AGG - chr8.1:43272194-43272213 MS.gene63127:CDS 60.0%
CCTCCAGCGGCATTTCTCAG+GGG - chr8.1:43272941-43272960 MS.gene63127:intron 60.0%
CGGACAGGGGCTGTTCTACA+TGG - chr8.1:43272150-43272169 MS.gene63127:CDS 60.0%
GAGCACTTCCACCAGCCGTT+AGG - chr8.1:43272103-43272122 MS.gene63127:CDS 60.0%
TGTGGCAGAACCGGATCCAG+CGG - chr8.1:43272381-43272400 MS.gene63127:intron 60.0%
! GGAGTTGAAGGAGGTGCAGG+AGG + chr8.1:43272361-43272380 None:intergenic 60.0%
ACGGAGAACCGGCTGGCAGT+TGG - chr8.1:43272983-43273002 MS.gene63127:intron 65.0%
CAGCGGCATTTCTCAGGGGC+GGG - chr8.1:43272945-43272964 MS.gene63127:intron 65.0%
CCAGCGGCATTTCTCAGGGG+CGG - chr8.1:43272944-43272963 MS.gene63127:intron 65.0%
CCCCTGAGAAATGCCGCTGG+AGG + chr8.1:43272944-43272963 None:intergenic 65.0%
CGGAGAACCGGCTGGCAGTT+GGG - chr8.1:43272984-43273003 MS.gene63127:intron 65.0%
GCTGGATCCGGTTCTGCCAC+AGG + chr8.1:43272382-43272401 None:intergenic 65.0%
CCGCCCCTGAGAAATGCCGC+TGG + chr8.1:43272947-43272966 None:intergenic 70.0%
CGGCATTTCTCAGGGGCGGG+TGG - chr8.1:43272948-43272967 MS.gene63127:intron 70.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 43271213 43273081 43271213 ID=MS.gene63127
chr8.1 mRNA 43271213 43273081 43271213 ID=MS.gene63127.t1;Parent=MS.gene63127
chr8.1 exon 43273031 43273081 43273031 ID=MS.gene63127.t1.exon1;Parent=MS.gene63127.t1
chr8.1 CDS 43273031 43273081 43273031 ID=cds.MS.gene63127.t1;Parent=MS.gene63127.t1
chr8.1 exon 43271580 43272344 43271580 ID=MS.gene63127.t1.exon2;Parent=MS.gene63127.t1
chr8.1 CDS 43271580 43272344 43271580 ID=cds.MS.gene63127.t1;Parent=MS.gene63127.t1
chr8.1 exon 43271213 43271476 43271213 ID=MS.gene63127.t1.exon3;Parent=MS.gene63127.t1
chr8.1 CDS 43271213 43271476 43271213 ID=cds.MS.gene63127.t1;Parent=MS.gene63127.t1
Gene Sequence

>MS.gene63127

ATGGCCGCGCTTACATTCAGGAAGTTCTTCACAACAATCGATCCAGACAAGGTAAGTGTGTCTTAATATTCACTAACTTTTGTTACTAAACTTTGAGTGTGGAGTAGATTTTTTAATCAAAAGTATGTTGATTGATTATTTTCCTGTCTCCCTTTTGGTATCCGGTAGATTTTGCATGGCTTCATTTGTTGTTACATGTTTAAGGATTTTGACTATGTTTGCCTTCTTGTTGGTTGGATGATGATGAATCTGTAAGTATAATTTATTTCCACTATTATGCACACACAAGAAACCGTATCTAAGATTTTGTGTACTCTGATTGCTAGATTGATAATTCCTGTTTGTTGTCACTCTGTAATGTTAGTTTGATATTTTCCTTGTCACTCTGCAATATATTTTGACCATGTTTGCCTTGTTGTTGGTTGGATGATGATGAATTGATAACTGTATTTTTTTCAACTATTATGCACACAAAAAAACCGTATCTACGATAATGTGTACTCTGATTGTTACTTTGATATTCCCAGTTTGTTGTCACTCTGTAATGTTAGTTTGAAATTTCCAGTTTGATGTCACTCTGTAAATCACATCGTCGTCAAATGACATCTCAATAAACATCAGTTGTATCTTAAGTAAATTATTTAAAAAAGAACACAGGTTTGTTATTTTCATGAGATATAGTATCTTTCAAATCATTTGTTGTAAAATGACTAATGAAGTTGTCACTTGTAATGTAGGATGAGGCTGATATTCCATCATACTTCAACAACCACTTTGGTGATGAACTACGACACAGAGTCTGGTTAGAAGACACAAGAGGTTCGAGGTTTGGTGTTTTCATAGGAAGTGAGGGGACGCGGACCTTTTTCTATAGGCTAAGGAGTTTGCTGGAGCACTTCCACCAGCCGTTAGGTCTCATACTGCGTCTAAGTTACAACGGACAGGGGCTGTTCTACATGGGTGCGCTTACACTCGACATGACCGAGCTAACGGTTCGTGAGAGGCGTACTTATGAACACCCTCCTTTCCTGATTGCTGCCGAGATACTGGATCCCTTGGAGGAGTATGAATTATACAATACCTCGTCATCCGATGAAGACAACGACGACGATGATTCCATCGATCTTGGCATTGATGTTGATGTTCCTCCTGCACCTCCTTCAACTCCTGTGGCAGAACCGGATCCAGCGGATGTTGATGTTGCACCCAATTCTCCTCCTCATCCCGAAGTTCATGATGCCAATCAACAACCACACAATGCCGATGATGCCCAAAATCCGCCTCCACATAATTTCTTTGATTTTGCTGCTGCCATGGATATCAACGATGAAGATGTGGAAGATGGAGCACAAAATCCTCCGCATAACTTCTTTGATGCTGCTGCTGTTGCCATGGGTAACAATGGTGAAAACATGGGTGCAGGTGGGAATGTGGCTAATAATGGGAGAGTGGAGAGCTGTGTTGTCAGACTTGCAGAATACATGGTGAAGACTAGCAATCTGGTAGGTGAATTGCATAACTGCTAATGCATAATGTAATTACTGTTATATAGTTTTTGGTAGATATGTCTAACGCCTGTTTTAAATTGTTATCATTTGCATGCAGTACCTACCAAGAAGATTTGTTGCACGAGTGTTTGGGAATGCAACACCGCCCACCATCCTACTTACAGATGCCATGGGCTTTGACTTTCCGTGCAGGATCAAGTGGAAGGACAGAGGCCATCTTCCTCCAGCGGCATTTCTCAGGGGCGGGTGGAAGAGATTTGTTACGGAGAACCGGCTGGCAGTTGGGAAAAGTGTTAGGTTGTCTGTCAACTTAGACAACCCATATGTTATGTATGCTAGGGAAGCTCGGATGGAGATTTGA
Protein sequence

>MS.gene63127.t1

MAALTFRKFFTTIDPDKDEADIPSYFNNHFGDELRHRVWLEDTRGSRFGVFIGSEGTRTFFYRLRSLLEHFHQPLGLILRLSYNGQGLFYMGALTLDMTELTVRERRTYEHPPFLIAAEILDPLEEYELYNTSSSDEDNDDDDSIDLGIDVDVPPAPPSTPVAEPDPADVDVAPNSPPHPEVHDANQQPHNADDAQNPPPHNFFDFAAAMDINDEDVEDGAQNPPHNFFDAAAVAMGNNGENMGAGGNVANNGRVESCVVRLAEYMVKTSNLYLPRRFVARVFGNATPPTILLTDAMGFDFPCRIKWKDRGHLPPAAFLRGGWKRFVTENRLAVGKSVRLSVNLDNPYVMYAREARMEI