Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63182.t1 | XP_013445809.1 | 81.4 | 279 | 49 | 1 | 1 | 279 | 1 | 276 | 1.60E-100 | 375.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63182.t1 | Q9LFQ3 | 40.8 | 103 | 58 | 2 | 111 | 211 | 328 | 429 | 5.3e-12 | 73.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63182.t1 | A0A072U246 | 81.4 | 279 | 49 | 1 | 1 | 279 | 1 | 276 | 1.1e-100 | 375.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene63182.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63182.t1 | MTR_8g469180 | 84.229 | 279 | 41 | 1 | 1 | 279 | 1 | 276 | 3.89e-170 | 472 |
| MS.gene63182.t1 | MTR_8g062700 | 55.172 | 87 | 39 | 0 | 193 | 279 | 332 | 418 | 3.11e-23 | 99.8 |
| MS.gene63182.t1 | MTR_8g062700 | 34.392 | 189 | 92 | 3 | 36 | 192 | 227 | 415 | 7.79e-23 | 98.6 |
| MS.gene63182.t1 | MTR_8g062700 | 64.384 | 73 | 26 | 0 | 193 | 265 | 332 | 404 | 2.71e-22 | 97.1 |
| MS.gene63182.t1 | MTR_8g062700 | 33.862 | 189 | 92 | 4 | 36 | 192 | 227 | 414 | 1.03e-20 | 92.4 |
| MS.gene63182.t1 | MTR_5g036670 | 50.000 | 86 | 41 | 1 | 195 | 278 | 320 | 405 | 4.34e-19 | 87.4 |
| MS.gene63182.t1 | MTR_5g036670 | 50.000 | 78 | 36 | 2 | 118 | 192 | 327 | 404 | 1.31e-13 | 71.2 |
| MS.gene63182.t1 | MTR_6g008970 | 46.237 | 93 | 50 | 0 | 187 | 279 | 332 | 424 | 4.98e-19 | 87.4 |
| MS.gene63182.t1 | MTR_6g008970 | 47.561 | 82 | 43 | 0 | 111 | 192 | 340 | 421 | 3.61e-17 | 82.0 |
| MS.gene63182.t1 | MTR_6g008995 | 38.255 | 149 | 54 | 3 | 79 | 189 | 197 | 345 | 8.15e-19 | 86.3 |
| MS.gene63182.t1 | MTR_6g008995 | 51.351 | 74 | 36 | 0 | 198 | 271 | 270 | 343 | 1.55e-17 | 82.4 |
| MS.gene63182.t1 | MTR_6g008995 | 38.255 | 149 | 54 | 3 | 79 | 189 | 140 | 288 | 8.80e-19 | 85.9 |
| MS.gene63182.t1 | MTR_6g008995 | 51.351 | 74 | 36 | 0 | 198 | 271 | 213 | 286 | 1.96e-17 | 82.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63182.t1 | AT5G15020 | 40.566 | 106 | 56 | 1 | 164 | 269 | 304 | 402 | 1.46e-16 | 80.1 |
| MS.gene63182.t1 | AT5G15020 | 42.045 | 88 | 49 | 1 | 111 | 196 | 328 | 415 | 3.27e-14 | 73.2 |
| MS.gene63182.t1 | AT5G15020 | 40.566 | 106 | 56 | 1 | 164 | 269 | 304 | 402 | 1.67e-16 | 80.1 |
| MS.gene63182.t1 | AT5G15020 | 42.045 | 88 | 49 | 1 | 111 | 196 | 328 | 415 | 3.49e-14 | 73.2 |
| MS.gene63182.t1 | AT3G01320 | 44.706 | 85 | 47 | 0 | 180 | 264 | 317 | 401 | 7.43e-16 | 78.2 |
| MS.gene63182.t1 | AT3G01320 | 40.385 | 104 | 58 | 3 | 111 | 211 | 332 | 434 | 6.57e-14 | 72.4 |
| MS.gene63182.t1 | AT3G01320 | 52.174 | 69 | 33 | 0 | 196 | 264 | 321 | 389 | 9.55e-16 | 77.8 |
| MS.gene63182.t1 | AT3G01320 | 38.393 | 112 | 65 | 3 | 103 | 211 | 312 | 422 | 5.71e-14 | 72.4 |
| MS.gene63182.t1 | AT1G24190 | 31.797 | 217 | 115 | 8 | 69 | 279 | 178 | 367 | 1.34e-15 | 77.4 |
| MS.gene63182.t1 | AT1G24190 | 31.797 | 217 | 115 | 8 | 69 | 279 | 178 | 367 | 1.79e-15 | 77.0 |
| MS.gene63182.t1 | AT1G24190 | 32.692 | 208 | 107 | 8 | 69 | 270 | 178 | 358 | 1.88e-15 | 77.0 |
| MS.gene63182.t1 | AT1G24190 | 41.772 | 79 | 45 | 1 | 115 | 193 | 288 | 365 | 5.01e-13 | 69.7 |
| MS.gene63182.t1 | AT1G70060 | 47.945 | 73 | 37 | 1 | 198 | 270 | 303 | 374 | 1.67e-14 | 73.9 |
| MS.gene63182.t1 | AT1G70060 | 47.945 | 73 | 37 | 1 | 198 | 270 | 303 | 374 | 1.67e-14 | 73.9 |
Find 55 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAACAGGTGGATGATTTAT+TGG | 0.144756 | 8.4:-40963348 | MS.gene63182:intron |
| CCGAGAGGAATTACAATTAT+TGG | 0.178616 | 8.4:-40963791 | MS.gene63182:intron |
| ATCAAACCTTTGTTAATTTC+TGG | 0.213353 | 8.4:+40970397 | None:intergenic |
| ATCAAACCTTTGTTAAATTC+TGG | 0.224271 | 8.4:+40970505 | None:intergenic |
| GTCAGCAGCTTCATTATCTT+TGG | 0.238299 | 8.4:+40972195 | None:intergenic |
| GGGAAAATATACCGATCTTA+TGG | 0.254179 | 8.4:-40963327 | MS.gene63182:CDS |
| CTTCAAAGCTTTAACAAGTA+TGG | 0.279212 | 8.4:+40963262 | None:intergenic |
| AAACAATCCTGAAAATTATC+AGG | 0.286860 | 8.4:-40963857 | MS.gene63182:CDS |
| AGTAGACAGCGTGGGGCGTT+TGG | 0.314226 | 8.4:+40972159 | None:intergenic |
| CAACAGGTGGATGATTTATT+GGG | 0.325941 | 8.4:-40963347 | MS.gene63182:intron |
| GTAGACAGCGTGGGGCGTTT+GGG | 0.348314 | 8.4:+40972160 | None:intergenic |
| CATACTTGTTAAAGCTTTGA+AGG | 0.355544 | 8.4:-40963261 | MS.gene63182:CDS |
| TTGCAAATCGTTGGCCTTCT+CGG | 0.356912 | 8.4:+40970561 | None:intergenic |
| CCTTTGTTAATTTCTGGATC+AGG | 0.378179 | 8.4:+40970403 | None:intergenic |
| GGAAAATATACCGATCTTAT+GGG | 0.409782 | 8.4:-40963326 | MS.gene63182:CDS |
| TTCATTAGGAATTTGTTTGT+CGG | 0.419300 | 8.4:-40970592 | MS.gene63182:intron |
| ACCTCTCCGTGACTTTGTTA+AGG | 0.425146 | 8.4:+40970208 | None:intergenic |
| CTTTCAGATAGGCGATTTGT+TGG | 0.441089 | 8.4:-40964820 | MS.gene63182:intron |
| TTCCCATTCTGTAGCTATGT+AGG | 0.447539 | 8.4:+40970148 | None:intergenic |
| TCCCATTCTGTAGCTATGTA+GGG | 0.475220 | 8.4:+40970149 | None:intergenic |
| TTCTTGAAACAGAAATGAAA+AGG | 0.481600 | 8.4:-40972219 | None:intergenic |
| GCGATCACCTTTCGTATCAA+TGG | 0.487977 | 8.4:+40970444 | None:intergenic |
| GTCCCTACATAGCTACAGAA+TGG | 0.496961 | 8.4:-40970151 | MS.gene63182:CDS |
| TTTCAGATAGGCGATTTGTT+GGG | 0.503365 | 8.4:-40964819 | MS.gene63182:intron |
| CATGACACAAAGTGAAAAGA+AGG | 0.508610 | 8.4:-40963288 | MS.gene63182:CDS |
| CCAATAATTGTAATTCCTCT+CGG | 0.508805 | 8.4:+40963791 | None:intergenic |
| AACAAAGTCACGGAGAGGTT+AGG | 0.509807 | 8.4:-40970204 | MS.gene63182:CDS |
| TCCCTACATAGCTACAGAAT+GGG | 0.512003 | 8.4:-40970150 | MS.gene63182:CDS |
| CAATAATTGTAATTCCTCTC+GGG | 0.513671 | 8.4:+40963792 | None:intergenic |
| AAGGTAAGAAAATGCTTCAC+TGG | 0.513839 | 8.4:+40970227 | None:intergenic |
| AGGTTTGATGAGTGCAAATG+AGG | 0.530693 | 8.4:-40970383 | MS.gene63182:CDS |
| TTTGGTGTCTCAGGTATGTA+CGG | 0.531859 | 8.4:-40963928 | MS.gene63182:intron |
| ATGATCACCTTGCAAATCGT+TGG | 0.554360 | 8.4:+40970552 | None:intergenic |
| ATACTTGTTAAAGCTTTGAA+GGG | 0.554714 | 8.4:-40963260 | MS.gene63182:CDS |
| ATTAAATTCGCCCATAAGAT+CGG | 0.555210 | 8.4:+40963316 | None:intergenic |
| TTCTTACCTTAACAAAGTCA+CGG | 0.564749 | 8.4:-40970214 | MS.gene63182:CDS |
| TTGACACAATGTGAATCCAA+TGG | 0.569616 | 8.4:-40964759 | MS.gene63182:intron |
| GTGTCTCAGGTATGTACGGT+TGG | 0.570587 | 8.4:-40963924 | MS.gene63182:intron |
| CTTGATCCAGAATTTAACAA+AGG | 0.573154 | 8.4:-40970511 | MS.gene63182:CDS |
| GGGAAAACATTCTGATCTTG+TGG | 0.578356 | 8.4:-40964799 | MS.gene63182:CDS |
| TCTTGTGTAGTAGACAGCGT+GGG | 0.582235 | 8.4:+40972151 | None:intergenic |
| AAACATTCTGATCTTGTGGA+GGG | 0.596716 | 8.4:-40964795 | MS.gene63182:CDS |
| CAAAGGTTTGATAAGTGCAG+AGG | 0.606415 | 8.4:-40970494 | MS.gene63182:CDS |
| CCTGATCCAGAAATTAACAA+AGG | 0.616646 | 8.4:-40970403 | MS.gene63182:CDS |
| AAGACAAGAACTACAAACAA+TGG | 0.616882 | 8.4:-40970118 | MS.gene63182:intron |
| AAAACATTCTGATCTTGTGG+AGG | 0.627867 | 8.4:-40964796 | MS.gene63182:CDS |
| GTCTTGTGTAGTAGACAGCG+TGG | 0.639850 | 8.4:+40972150 | None:intergenic |
| GATCAGGATGTTCGACAATG+AGG | 0.651290 | 8.4:+40970419 | None:intergenic |
| TGTCGGACTGAGTACCGAGA+AGG | 0.658623 | 8.4:-40970575 | MS.gene63182:CDS |
| GAGAAGGCCAACGATTTGCA+AGG | 0.661652 | 8.4:-40970559 | MS.gene63182:CDS |
| ACCTTAACAAAGTCACGGAG+AGG | 0.667295 | 8.4:-40970209 | MS.gene63182:CDS |
| CTTGTGTAGTAGACAGCGTG+GGG | 0.673923 | 8.4:+40972152 | None:intergenic |
| CAGCAAAATAATTACCCGAG+AGG | 0.690212 | 8.4:-40963806 | MS.gene63182:CDS |
| GACATGACCATTGATACGAA+AGG | 0.732031 | 8.4:-40970451 | MS.gene63182:CDS |
| GGAGAGGTTAGGAATACACG+AGG | 0.778238 | 8.4:-40970193 | MS.gene63182:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 40963247 | 40972227 | 40963247 | ID=MS.gene63182 |
| chr8.4 | mRNA | 40963247 | 40972227 | 40963247 | ID=MS.gene63182.t1;Parent=MS.gene63182 |
| chr8.4 | exon | 40972148 | 40972227 | 40972148 | ID=MS.gene63182.t1.exon1;Parent=MS.gene63182.t1 |
| chr8.4 | CDS | 40972148 | 40972227 | 40972148 | ID=cds.MS.gene63182.t1;Parent=MS.gene63182.t1 |
| chr8.4 | exon | 40970353 | 40970606 | 40970353 | ID=MS.gene63182.t1.exon2;Parent=MS.gene63182.t1 |
| chr8.4 | CDS | 40970353 | 40970606 | 40970353 | ID=cds.MS.gene63182.t1;Parent=MS.gene63182.t1 |
| chr8.4 | exon | 40970119 | 40970255 | 40970119 | ID=MS.gene63182.t1.exon3;Parent=MS.gene63182.t1 |
| chr8.4 | CDS | 40970119 | 40970255 | 40970119 | ID=cds.MS.gene63182.t1;Parent=MS.gene63182.t1 |
| chr8.4 | exon | 40964760 | 40964835 | 40964760 | ID=MS.gene63182.t1.exon4;Parent=MS.gene63182.t1 |
| chr8.4 | CDS | 40964760 | 40964835 | 40964760 | ID=cds.MS.gene63182.t1;Parent=MS.gene63182.t1 |
| chr8.4 | exon | 40964644 | 40964673 | 40964644 | ID=MS.gene63182.t1.exon5;Parent=MS.gene63182.t1 |
| chr8.4 | CDS | 40964644 | 40964673 | 40964644 | ID=cds.MS.gene63182.t1;Parent=MS.gene63182.t1 |
| chr8.4 | exon | 40963792 | 40963937 | 40963792 | ID=MS.gene63182.t1.exon6;Parent=MS.gene63182.t1 |
| chr8.4 | CDS | 40963792 | 40963937 | 40963792 | ID=cds.MS.gene63182.t1;Parent=MS.gene63182.t1 |
| chr8.4 | exon | 40963247 | 40963363 | 40963247 | ID=MS.gene63182.t1.exon7;Parent=MS.gene63182.t1 |
| chr8.4 | CDS | 40963247 | 40963363 | 40963247 | ID=cds.MS.gene63182.t1;Parent=MS.gene63182.t1 |
| Gene Sequence |
| Protein sequence |
