Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63190.t1 | AES88592.2 | 74.7 | 99 | 16 | 1 | 5 | 94 | 69 | 167 | 1.70E-33 | 151.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63190.t1 | Q8L7S3 | 48.5 | 99 | 39 | 3 | 6 | 92 | 80 | 178 | 5.5e-17 | 88.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63190.t1 | G7JPD7 | 74.7 | 99 | 16 | 1 | 5 | 94 | 69 | 167 | 1.2e-33 | 151.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene63190.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63190.t1 | MTR_4g059550 | 72.727 | 99 | 18 | 1 | 5 | 94 | 69 | 167 | 8.88e-36 | 128 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63190.t1 | AT2G28450 | 46.078 | 102 | 41 | 3 | 6 | 94 | 80 | 180 | 1.34e-20 | 85.1 |
| MS.gene63190.t1 | AT2G28450 | 48.515 | 101 | 40 | 3 | 6 | 94 | 80 | 180 | 1.38e-20 | 85.1 |
Find 23 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTCCCTCGCTCGGAAGTT+GGG | 0.317508 | 8.4:+41139586 | None:intergenic |
| TTCCTCCCTCGCTCGGAAGT+TGG | 0.334787 | 8.4:+41139585 | None:intergenic |
| GTTGGGTCCCACGTGTTATA+CGG | 0.350671 | 8.4:+41139603 | None:intergenic |
| CTCGCTATGCGCGTAACAGC+AGG | 0.445517 | 8.4:+41139644 | None:intergenic |
| AAACCTCACCACATCCTCAT+AGG | 0.458278 | 8.4:+41139444 | None:intergenic |
| CAATGCCTTCCTCCCTCGCT+CGG | 0.468392 | 8.4:+41139578 | None:intergenic |
| AAGGCATTGAAATCTATTAC+CGG | 0.477283 | 8.4:-41139564 | MS.gene63190:CDS |
| CGCATAGCGAGGAAGAGCTT+CGG | 0.503251 | 8.4:-41139632 | MS.gene63190:CDS |
| AACTTCCGAGCGAGGGAGGA+AGG | 0.509520 | 8.4:-41139583 | MS.gene63190:CDS |
| GCAGGTATCGTCGTGACTGC+AGG | 0.509824 | 8.4:+41139662 | None:intergenic |
| GTTGTTGTTGATGAAGTTGA+CGG | 0.516486 | 8.4:-41139531 | MS.gene63190:CDS |
| GGTGACGACTGCGGAGAAGA+AGG | 0.533901 | 8.4:-41139510 | MS.gene63190:CDS |
| ACCCAACTTCCGAGCGAGGG+AGG | 0.596059 | 8.4:-41139587 | MS.gene63190:CDS |
| CGGCGATGGCAAACTCGATG+TGG | 0.606835 | 8.4:-41139722 | None:intergenic |
| TGGGACCCAACTTCCGAGCG+AGG | 0.607008 | 8.4:-41139591 | MS.gene63190:CDS |
| TCGATGTGGAAGACAAGTCT+CGG | 0.607387 | 8.4:-41139708 | MS.gene63190:CDS |
| GGGACCCAACTTCCGAGCGA+GGG | 0.618617 | 8.4:-41139590 | MS.gene63190:CDS |
| CTGCTGTTACGCGCATAGCG+AGG | 0.626088 | 8.4:-41139643 | MS.gene63190:CDS |
| GGCAATGTCCGTATAACACG+TGG | 0.633361 | 8.4:-41139611 | MS.gene63190:CDS |
| GGTATCGTCGTGACTGCAGG+AGG | 0.643618 | 8.4:+41139665 | None:intergenic |
| TTTCCTATGAGGATGTGGTG+AGG | 0.664216 | 8.4:-41139447 | None:intergenic |
| GCAATGTCCGTATAACACGT+GGG | 0.731198 | 8.4:-41139610 | MS.gene63190:CDS |
| GAAGTTGACGGTGACGACTG+CGG | 0.731958 | 8.4:-41139519 | MS.gene63190:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACAACAACAATTTTCTCAC+CGG | + | chr8.4:41139624-41139643 | None:intergenic | 30.0% |
| ! | GTTTAGTACATTTTCCTATG+AGG | - | chr8.4:41139708-41139727 | MS.gene63190:CDS | 30.0% |
| !! | AAGGCATTGAAATCTATTAC+CGG | - | chr8.4:41139602-41139621 | MS.gene63190:CDS | 30.0% |
| ! | GTTGTTGTTGATGAAGTTGA+CGG | - | chr8.4:41139635-41139654 | MS.gene63190:CDS | 35.0% |
| ! | TACATTTTCCTATGAGGATG+TGG | - | chr8.4:41139714-41139733 | MS.gene63190:CDS | 35.0% |
| !!! | GGAGGAATGTTTTCTGAAGT+AGG | + | chr8.4:41139486-41139505 | None:intergenic | 40.0% |
| GCAATGTCCGTATAACACGT+GGG | - | chr8.4:41139556-41139575 | MS.gene63190:CDS | 45.0% | |
| TCGATGTGGAAGACAAGTCT+CGG | - | chr8.4:41139458-41139477 | MS.gene63190:CDS | 45.0% | |
| GGCAATGTCCGTATAACACG+TGG | - | chr8.4:41139555-41139574 | MS.gene63190:CDS | 50.0% | |
| GTTGGGTCCCACGTGTTATA+CGG | + | chr8.4:41139566-41139585 | None:intergenic | 50.0% | |
| CGCATAGCGAGGAAGAGCTT+CGG | - | chr8.4:41139534-41139553 | MS.gene63190:CDS | 55.0% | |
| GAAGTTGACGGTGACGACTG+CGG | - | chr8.4:41139647-41139666 | MS.gene63190:CDS | 55.0% | |
| AACTTCCGAGCGAGGGAGGA+AGG | - | chr8.4:41139583-41139602 | MS.gene63190:CDS | 60.0% | |
| CAATGCCTTCCTCCCTCGCT+CGG | + | chr8.4:41139591-41139610 | None:intergenic | 60.0% | |
| CTCGCTATGCGCGTAACAGC+AGG | + | chr8.4:41139525-41139544 | None:intergenic | 60.0% | |
| CTGCTGTTACGCGCATAGCG+AGG | - | chr8.4:41139523-41139542 | MS.gene63190:CDS | 60.0% | |
| GGTATCGTCGTGACTGCAGG+AGG | + | chr8.4:41139504-41139523 | None:intergenic | 60.0% | |
| GGTGACGACTGCGGAGAAGA+AGG | - | chr8.4:41139656-41139675 | MS.gene63190:CDS | 60.0% | |
| TCCTCCCTCGCTCGGAAGTT+GGG | + | chr8.4:41139583-41139602 | None:intergenic | 60.0% | |
| TTCCTCCCTCGCTCGGAAGT+TGG | + | chr8.4:41139584-41139603 | None:intergenic | 60.0% | |
| ! | GCAGGTATCGTCGTGACTGC+AGG | + | chr8.4:41139507-41139526 | None:intergenic | 60.0% |
| ACCCAACTTCCGAGCGAGGG+AGG | - | chr8.4:41139579-41139598 | MS.gene63190:CDS | 65.0% | |
| GGGACCCAACTTCCGAGCGA+GGG | - | chr8.4:41139576-41139595 | MS.gene63190:CDS | 65.0% | |
| TGGGACCCAACTTCCGAGCG+AGG | - | chr8.4:41139575-41139594 | MS.gene63190:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 41139449 | 41139739 | 41139449 | ID=MS.gene63190 |
| chr8.4 | mRNA | 41139449 | 41139739 | 41139449 | ID=MS.gene63190.t1;Parent=MS.gene63190 |
| chr8.4 | exon | 41139449 | 41139739 | 41139449 | ID=MS.gene63190.t1.exon1;Parent=MS.gene63190.t1 |
| chr8.4 | CDS | 41139449 | 41139739 | 41139449 | ID=cds.MS.gene63190.t1;Parent=MS.gene63190.t1 |
| Gene Sequence |
| Protein sequence |
