Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63305.t1 | XP_013460707.1 | 94.2 | 260 | 14 | 1 | 1 | 259 | 1 | 260 | 2.10E-139 | 505 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63305.t1 | O80942 | 50.4 | 113 | 43 | 3 | 33 | 135 | 18 | 127 | 2.5e-16 | 87.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63305.t1 | A0A072UZ72 | 94.2 | 260 | 14 | 1 | 1 | 259 | 1 | 260 | 1.5e-139 | 505.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene63305.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63305.t1 | MTR_3g068095 | 94.231 | 260 | 14 | 1 | 1 | 259 | 1 | 260 | 0.0 | 516 |
| MS.gene63305.t1 | MTR_5g080660 | 47.674 | 258 | 93 | 13 | 8 | 259 | 11 | 232 | 7.47e-52 | 169 |
| MS.gene63305.t1 | MTR_4g021270 | 56.311 | 103 | 31 | 3 | 33 | 121 | 36 | 138 | 1.10e-29 | 114 |
| MS.gene63305.t1 | MTR_5g014400 | 71.930 | 57 | 15 | 1 | 33 | 89 | 59 | 114 | 3.56e-23 | 95.1 |
| MS.gene63305.t1 | MTR_2g076060 | 49.398 | 83 | 39 | 1 | 10 | 89 | 2 | 84 | 2.88e-19 | 84.0 |
| MS.gene63305.t1 | MTR_1g070265 | 80.952 | 42 | 8 | 0 | 49 | 90 | 29 | 70 | 9.56e-18 | 79.0 |
| MS.gene63305.t1 | MTR_7g063220 | 70.000 | 50 | 15 | 0 | 55 | 104 | 24 | 73 | 5.33e-16 | 73.2 |
| MS.gene63305.t1 | MTR_4g064873 | 62.500 | 48 | 18 | 0 | 54 | 101 | 20 | 67 | 1.25e-14 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63305.t1 | AT2G37740 | 75.439 | 57 | 11 | 1 | 33 | 89 | 18 | 71 | 5.12e-24 | 98.6 |
| MS.gene63305.t1 | AT4G17810 | 30.804 | 224 | 113 | 4 | 30 | 253 | 19 | 200 | 8.05e-24 | 95.9 |
| MS.gene63305.t1 | AT5G06070 | 37.603 | 242 | 106 | 10 | 25 | 259 | 22 | 225 | 5.81e-23 | 94.0 |
| MS.gene63305.t1 | AT4G17810 | 69.091 | 55 | 17 | 0 | 30 | 84 | 19 | 73 | 1.03e-22 | 92.0 |
| MS.gene63305.t1 | AT3G23130 | 82.927 | 41 | 7 | 0 | 47 | 87 | 37 | 77 | 4.81e-18 | 80.5 |
| MS.gene63305.t1 | AT2G42410 | 78.049 | 41 | 9 | 0 | 48 | 88 | 40 | 80 | 2.62e-17 | 78.6 |
| MS.gene63305.t1 | AT2G42410 | 78.049 | 41 | 9 | 0 | 48 | 88 | 40 | 80 | 2.62e-17 | 78.6 |
| MS.gene63305.t1 | AT2G42410 | 78.049 | 41 | 9 | 0 | 48 | 88 | 66 | 106 | 2.85e-17 | 79.0 |
| MS.gene63305.t1 | AT3G09290 | 63.636 | 44 | 16 | 0 | 55 | 98 | 33 | 76 | 9.90e-13 | 65.1 |
| MS.gene63305.t1 | AT3G53820 | 68.571 | 35 | 11 | 0 | 55 | 89 | 19 | 53 | 3.43e-11 | 60.1 |
Find 47 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATATTCTTCTTCAATTAGA+TGG | 0.176609 | 3.3:-60342304 | MS.gene63305:CDS |
| TGTTGAAACAAGTTTGTCTA+TGG | 0.217128 | 3.3:-60342192 | MS.gene63305:CDS |
| TTCCCAAGACTTGTTGTTGA+TGG | 0.233876 | 3.3:+60342691 | None:intergenic |
| TTTAGGTCTGCTCAAGCTTT+AGG | 0.265540 | 3.3:-60342578 | MS.gene63305:CDS |
| AATGAAGTTCTAATTCATTA+TGG | 0.326510 | 3.3:+60342498 | None:intergenic |
| GGACAAAATAATTCAATGTT+TGG | 0.329063 | 3.3:-60342170 | MS.gene63305:CDS |
| AGATCCAAGTCTTCTATTCC+AGG | 0.334055 | 3.3:+60341981 | None:intergenic |
| GAAGAAATGGAAGCTGTCTT+TGG | 0.357856 | 3.3:+60342086 | None:intergenic |
| GGATACTTGGTGGATGTATA+TGG | 0.358230 | 3.3:-60342640 | MS.gene63305:CDS |
| CAGTCTGCAGAGTTTGTGGT+TGG | 0.405826 | 3.3:-60342011 | MS.gene63305:CDS |
| TAAATATGATGTGGATGAAA+AGG | 0.412314 | 3.3:-60342760 | MS.gene63305:CDS |
| GACAGGCAGTGAAGAAGAAA+TGG | 0.412947 | 3.3:+60342073 | None:intergenic |
| TCTTGTGTAGATGCAGTTGA+TGG | 0.423703 | 3.3:+60342353 | None:intergenic |
| AATGAAGGAGTGCCATCATT+TGG | 0.429411 | 3.3:-60342716 | MS.gene63305:CDS |
| GAAGAATATGAAGACATGTT+TGG | 0.429891 | 3.3:+60342317 | None:intergenic |
| ATATTCTTCTTCAATTAGAT+GGG | 0.438910 | 3.3:-60342303 | MS.gene63305:CDS |
| GAAGATGCAGCTAGGATACT+TGG | 0.443307 | 3.3:-60342653 | MS.gene63305:CDS |
| GAACCATCAACAACAAGTCT+TGG | 0.447185 | 3.3:-60342694 | MS.gene63305:CDS |
| ACTTCTGTAAAAGAGAGTTT+AGG | 0.451370 | 3.3:-60342595 | MS.gene63305:CDS |
| TTGGATCTTGAACTCAGGCT+TGG | 0.454061 | 3.3:-60341966 | MS.gene63305:CDS |
| TGGATCTTGAACTCAGGCTT+GGG | 0.459255 | 3.3:-60341965 | MS.gene63305:CDS |
| AAACATGGCTTCAGCAAGAC+AGG | 0.470524 | 3.3:+60342056 | None:intergenic |
| TTTGTGGTTGGAGTTAAGCC+TGG | 0.483977 | 3.3:-60341999 | MS.gene63305:CDS |
| TTGTTGATGGTTCCAAATGA+TGG | 0.490417 | 3.3:+60342704 | None:intergenic |
| AACCATCAACAACAAGTCTT+GGG | 0.510991 | 3.3:-60342693 | MS.gene63305:CDS |
| GTTGAAACAAGTTTGTCTAT+GGG | 0.512192 | 3.3:-60342191 | MS.gene63305:CDS |
| AAGTGACATGAGTAAGATCT+TGG | 0.519917 | 3.3:+60342614 | None:intergenic |
| AAGACTTGGATCTTGAACTC+AGG | 0.538252 | 3.3:-60341971 | MS.gene63305:CDS |
| GAGTCTCTTGATTCTGAACA+AGG | 0.543085 | 3.3:-60342266 | MS.gene63305:CDS |
| AGGTCTGCTCAAGCTTTAGG+TGG | 0.544624 | 3.3:-60342575 | MS.gene63305:CDS |
| AACAAGGGCTAGCTAATGCA+AGG | 0.555187 | 3.3:-60342250 | MS.gene63305:CDS |
| TAAGCCTGGAATAGAAGACT+TGG | 0.563435 | 3.3:-60341985 | MS.gene63305:CDS |
| ATCTTGCACTCACACAATGA+AGG | 0.564898 | 3.3:-60342731 | MS.gene63305:CDS |
| GCAGAGATAACTCTTCTACT+TGG | 0.579663 | 3.3:-60342376 | MS.gene63305:CDS |
| TATTCTTCTTCAATTAGATG+GGG | 0.582180 | 3.3:-60342302 | MS.gene63305:CDS |
| GATGCAGCTAGGATACTTGG+TGG | 0.596955 | 3.3:-60342650 | MS.gene63305:CDS |
| TGACATGAGTAAGATCTTGG+TGG | 0.606437 | 3.3:+60342617 | None:intergenic |
| ACTGATGATGATTCATGTGG+TGG | 0.616045 | 3.3:-60342134 | MS.gene63305:CDS |
| CTGATGATGATTCATGTGGT+GGG | 0.621213 | 3.3:-60342133 | MS.gene63305:CDS |
| ATTCTTCTTCAATTAGATGG+GGG | 0.625385 | 3.3:-60342301 | MS.gene63305:CDS |
| AGGAATCACTAAATATGATG+TGG | 0.652487 | 3.3:-60342769 | MS.gene63305:CDS |
| AAACATCTATCATTCAAACA+TGG | 0.662081 | 3.3:+60342041 | None:intergenic |
| TGATGATGATTCATGTGGTG+GGG | 0.671193 | 3.3:-60342132 | MS.gene63305:CDS |
| AGTCTCTTGATTCTGAACAA+GGG | 0.707551 | 3.3:-60342265 | MS.gene63305:CDS |
| ACAAGGGCTAGCTAATGCAA+GGG | 0.711358 | 3.3:-60342249 | MS.gene63305:CDS |
| CAAACTGATGATGATTCATG+TGG | 0.716039 | 3.3:-60342137 | MS.gene63305:CDS |
| CAGAATCAAGAGACTCCATG+TGG | 0.748859 | 3.3:+60342273 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATTCTTCTTCAATTAGAT+GGG | - | chr3.3:60342414-60342433 | MS.gene63305:intron | 20.0% |
| !! | CTAATTATTGTTCTAACTTA+TGG | - | chr3.3:60342304-60342323 | MS.gene63305:CDS | 20.0% |
| !!! | AATGAAGTTCTAATTCATTA+TGG | + | chr3.3:60342222-60342241 | None:intergenic | 20.0% |
| ! | AAACATCTATCATTCAAACA+TGG | + | chr3.3:60342679-60342698 | None:intergenic | 25.0% |
| ! | CATATTCTTCTTCAATTAGA+TGG | - | chr3.3:60342413-60342432 | MS.gene63305:intron | 25.0% |
| ! | GGACAAAATAATTCAATGTT+TGG | - | chr3.3:60342547-60342566 | MS.gene63305:CDS | 25.0% |
| ! | TAAATATGATGTGGATGAAA+AGG | - | chr3.3:60341957-60341976 | MS.gene63305:CDS | 25.0% |
| ! | TATTCTTCTTCAATTAGATG+GGG | - | chr3.3:60342415-60342434 | MS.gene63305:intron | 25.0% |
| !! | GAACAAATTTTCAAAGGTTA+TGG | - | chr3.3:60342490-60342509 | MS.gene63305:CDS | 25.0% |
| ACTTCTGTAAAAGAGAGTTT+AGG | - | chr3.3:60342122-60342141 | MS.gene63305:CDS | 30.0% | |
| AGGAATCACTAAATATGATG+TGG | - | chr3.3:60341948-60341967 | MS.gene63305:CDS | 30.0% | |
| ATTCTTCTTCAATTAGATGG+GGG | - | chr3.3:60342416-60342435 | MS.gene63305:intron | 30.0% | |
| GAAGAATATGAAGACATGTT+TGG | + | chr3.3:60342403-60342422 | None:intergenic | 30.0% | |
| GTTGAAACAAGTTTGTCTAT+GGG | - | chr3.3:60342526-60342545 | MS.gene63305:CDS | 30.0% | |
| TGTTGAAACAAGTTTGTCTA+TGG | - | chr3.3:60342525-60342544 | MS.gene63305:CDS | 30.0% | |
| ! | CATCATCAGTTTGCAATTTT+TGG | + | chr3.3:60342573-60342592 | None:intergenic | 30.0% |
| AACCATCAACAACAAGTCTT+GGG | - | chr3.3:60342024-60342043 | MS.gene63305:CDS | 35.0% | |
| AAGTGACATGAGTAAGATCT+TGG | + | chr3.3:60342106-60342125 | None:intergenic | 35.0% | |
| AGTCTCTTGATTCTGAACAA+GGG | - | chr3.3:60342452-60342471 | MS.gene63305:intron | 35.0% | |
| ! | CAAACTGATGATGATTCATG+TGG | - | chr3.3:60342580-60342599 | MS.gene63305:CDS | 35.0% |
| ! | GCAAGGGAACAAATTTTCAA+AGG | - | chr3.3:60342484-60342503 | MS.gene63305:CDS | 35.0% |
| !! | TTGTTGATGGTTCCAAATGA+TGG | + | chr3.3:60342016-60342035 | None:intergenic | 35.0% |
| AAGACTTGGATCTTGAACTC+AGG | - | chr3.3:60342746-60342765 | MS.gene63305:CDS | 40.0% | |
| AGATCCAAGTCTTCTATTCC+AGG | + | chr3.3:60342739-60342758 | None:intergenic | 40.0% | |
| ATCTTGCACTCACACAATGA+AGG | - | chr3.3:60341986-60342005 | MS.gene63305:CDS | 40.0% | |
| GAACCATCAACAACAAGTCT+TGG | - | chr3.3:60342023-60342042 | MS.gene63305:CDS | 40.0% | |
| GAAGAAATGGAAGCTGTCTT+TGG | + | chr3.3:60342634-60342653 | None:intergenic | 40.0% | |
| GAGTCTCTTGATTCTGAACA+AGG | - | chr3.3:60342451-60342470 | MS.gene63305:intron | 40.0% | |
| GCAGAGATAACTCTTCTACT+TGG | - | chr3.3:60342341-60342360 | MS.gene63305:CDS | 40.0% | |
| TAAGCCTGGAATAGAAGACT+TGG | - | chr3.3:60342732-60342751 | MS.gene63305:CDS | 40.0% | |
| TCTTGTGTAGATGCAGTTGA+TGG | + | chr3.3:60342367-60342386 | None:intergenic | 40.0% | |
| TGACATGAGTAAGATCTTGG+TGG | + | chr3.3:60342103-60342122 | None:intergenic | 40.0% | |
| TTTAGGTCTGCTCAAGCTTT+AGG | - | chr3.3:60342139-60342158 | MS.gene63305:CDS | 40.0% | |
| ! | ACTGATGATGATTCATGTGG+TGG | - | chr3.3:60342583-60342602 | MS.gene63305:CDS | 40.0% |
| ! | CTGATGATGATTCATGTGGT+GGG | - | chr3.3:60342584-60342603 | MS.gene63305:CDS | 40.0% |
| ! | GGATACTTGGTGGATGTATA+TGG | - | chr3.3:60342077-60342096 | MS.gene63305:CDS | 40.0% |
| ! | TGATGATGATTCATGTGGTG+GGG | - | chr3.3:60342585-60342604 | MS.gene63305:CDS | 40.0% |
| ! | TTTTCAGTCTGCAGAGTTTG+TGG | - | chr3.3:60342702-60342721 | MS.gene63305:CDS | 40.0% |
| !! | AATGAAGGAGTGCCATCATT+TGG | - | chr3.3:60342001-60342020 | MS.gene63305:CDS | 40.0% |
| !! | TTCCCAAGACTTGTTGTTGA+TGG | + | chr3.3:60342029-60342048 | None:intergenic | 40.0% |
| AAACATGGCTTCAGCAAGAC+AGG | + | chr3.3:60342664-60342683 | None:intergenic | 45.0% | |
| CAGAATCAAGAGACTCCATG+TGG | + | chr3.3:60342447-60342466 | None:intergenic | 45.0% | |
| GAAGATGCAGCTAGGATACT+TGG | - | chr3.3:60342064-60342083 | MS.gene63305:CDS | 45.0% | |
| GACAGGCAGTGAAGAAGAAA+TGG | + | chr3.3:60342647-60342666 | None:intergenic | 45.0% | |
| TAGATGGGGGAATAACCACA+TGG | - | chr3.3:60342429-60342448 | MS.gene63305:intron | 45.0% | |
| TGGATCTTGAACTCAGGCTT+GGG | - | chr3.3:60342752-60342771 | MS.gene63305:CDS | 45.0% | |
| TTGGATCTTGAACTCAGGCT+TGG | - | chr3.3:60342751-60342770 | MS.gene63305:CDS | 45.0% | |
| TTTGTGGTTGGAGTTAAGCC+TGG | - | chr3.3:60342718-60342737 | MS.gene63305:CDS | 45.0% | |
| ! | CTTTTGCAGAAGATGCAGCT+AGG | - | chr3.3:60342056-60342075 | MS.gene63305:CDS | 45.0% |
| !! | AACAAGGGCTAGCTAATGCA+AGG | - | chr3.3:60342467-60342486 | MS.gene63305:intron | 45.0% |
| !! | ACAAGGGCTAGCTAATGCAA+GGG | - | chr3.3:60342468-60342487 | MS.gene63305:intron | 45.0% |
| CAGTCTGCAGAGTTTGTGGT+TGG | - | chr3.3:60342706-60342725 | MS.gene63305:CDS | 50.0% | |
| GATGCAGCTAGGATACTTGG+TGG | - | chr3.3:60342067-60342086 | MS.gene63305:CDS | 50.0% | |
| !! | AGGTCTGCTCAAGCTTTAGG+TGG | - | chr3.3:60342142-60342161 | MS.gene63305:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 60341944 | 60342795 | 60341944 | ID=MS.gene63305 |
| chr3.3 | mRNA | 60341944 | 60342795 | 60341944 | ID=MS.gene63305.t1;Parent=MS.gene63305 |
| chr3.3 | exon | 60342480 | 60342795 | 60342480 | ID=MS.gene63305.t1.exon1;Parent=MS.gene63305.t1 |
| chr3.3 | CDS | 60342480 | 60342795 | 60342480 | ID=cds.MS.gene63305.t1;Parent=MS.gene63305.t1 |
| chr3.3 | exon | 60341944 | 60342407 | 60341944 | ID=MS.gene63305.t1.exon2;Parent=MS.gene63305.t1 |
| chr3.3 | CDS | 60341944 | 60342407 | 60341944 | ID=cds.MS.gene63305.t1;Parent=MS.gene63305.t1 |
| Gene Sequence |
| Protein sequence |
