AlfalfaGEDB | MS.gene63324

M. sativa cultivar XinJiangDaYe / MS.gene63324

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0046983 GO:0097159 GO:1901363
PFAM: PF00319.21

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63324.t1	KEH34708.1	53.6	166	44	5	1	146	1	153	4.20E-27	131.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63324.t1	Q9FKK2	76.7	43	10	0	85	127	16	58	9.5e-12	71.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63324.t1	A0A072UZY9	53.6	166	44	5	1	146	1	153	3.0e-27	131.3

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene63324.t1	TF	MADS-M-type

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63324.t1	MTR_3g067870	60.000	140	30	4	1	127	1	127	1.18e-45	147
MS.gene63324.t1	MTR_5g045560	63.235	68	22	1	83	150	16	80	1.61e-21	87.8
MS.gene63324.t1	MTR_2g085250	61.194	67	23	1	83	149	16	79	2.55e-20	84.7
MS.gene63324.t1	MTR_2g085280	61.194	67	23	1	83	149	16	79	2.68e-20	84.7
MS.gene63324.t1	MTR_1g012570	64.615	65	20	1	83	147	16	77	4.00e-20	84.0
MS.gene63324.t1	MTR_1g105905	63.492	63	20	1	85	147	1	60	8.84e-20	82.8
MS.gene63324.t1	MTR_1g105910	61.538	65	22	1	83	147	16	77	8.87e-20	83.2
MS.gene63324.t1	MTR_1g077360	60.000	65	23	1	83	147	16	77	1.16e-19	82.8
MS.gene63324.t1	MTR_8g043650	61.538	65	22	1	83	147	16	77	1.28e-19	82.8
MS.gene63324.t1	MTR_3g052920	82.222	45	8	0	83	127	16	60	1.39e-19	82.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63324.t1	AT5G60440	76.744	43	10	0	85	127	16	58	2.75e-17	77.4
MS.gene63324.t1	AT1G01530	68.182	44	14	0	85	128	16	59	6.03e-15	70.5
MS.gene63324.t1	AT4G36590	58.333	60	22	1	83	142	14	70	1.16e-14	69.7
MS.gene63324.t1	AT1G65360	45.714	70	34	2	85	154	16	81	4.56e-13	65.1
MS.gene63324.t1	AT2G24840	62.791	43	16	0	85	127	72	114	5.75e-13	65.5

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Find 24 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTGGATGAAGAAGCAAG+AGG	0.708930	3.3:+60019913	None:intergenic
AAATATGGTTGAAAATCATA+AGG	0.438033	3.3:+60020176	MS.gene63324:CDS
AATGAAAATACCTTCTCACC+AGG	0.623617	3.3:-60020673	None:intergenic
AATTTATATACAGGGAGAAA+AGG	0.506795	3.3:+60020288	MS.gene63324:intron
ACTTTCTCGAAGCGCCGCAG+TGG	0.564118	3.3:+60020583	MS.gene63324:CDS
ACTTTCTCGAAGCGTCGTAG+TGG	0.570157	3.3:-60021149	None:intergenic
AGATGAAGAATTCGCAAATA+TGG	0.383002	3.3:+60020161	MS.gene63324:CDS
AGCAACATATGCACCACAGA+GGG	0.625524	3.3:-60020635	None:intergenic
AGCAAGCTTTGCACCCTCTG+TGG	0.545328	3.3:+60020622	MS.gene63324:CDS
ATCTCTTCAGATCGCTGCTC+TGG	0.413157	3.3:+60020125	MS.gene63324:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTGGATATGAAACAAAAGT+TGG	+	chr3.3:60020348-60020367	MS.gene63324:intron	30.0%
AGATGAAGAATTCGCAAATA+TGG	+	chr3.3:60020161-60020180	MS.gene63324:CDS	30.0%
ATCATATTCATCCCATCAAT+GGG	+	chr3.3:60020427-60020446	MS.gene63324:intron	30.0%
CAGTAACAAAACAAAAGGAA+AGG	-	chr3.3:60020211-60020230	None:intergenic	30.0%
CTGTGTTGAATAATTCAAGT+TGG	+	chr3.3:60020404-60020423	MS.gene63324:intron	30.0%
GATCATATTCATCATACCAA+TGG	+	chr3.3:60020896-60020915	MS.gene63324:intron	30.0%
GATGAAATGCAAAATTCTGA+AGG	+	chr3.3:60019950-60019969	MS.gene63324:CDS	30.0%
TGAAGGACAAAATCATCATA+TGG	+	chr3.3:60019967-60019986	MS.gene63324:CDS	30.0%
AAAAGTTGGAATGGGAGATT+GGG	+	chr3.3:60020362-60020381	MS.gene63324:intron	35.0%
AATGAAAATACCTTCTCACC+AGG	-	chr3.3:60020676-60020695	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	60019920	60021255	60019920	ID=MS.gene63324
chr3.3	mRNA	60019920	60021255	60019920	ID=MS.gene63324.t1;Parent=MS.gene63324
chr3.3	exon	60019920	60019988	60019920	ID=MS.gene63324.t1.exon1;Parent=MS.gene63324.t1
chr3.3	CDS	60019920	60019988	60019920	ID=cds.MS.gene63324.t1;Parent=MS.gene63324.t1
chr3.3	exon	60020108	60020197	60020108	ID=MS.gene63324.t1.exon2;Parent=MS.gene63324.t1
chr3.3	CDS	60020108	60020197	60020108	ID=cds.MS.gene63324.t1;Parent=MS.gene63324.t1
chr3.3	exon	60020301	60020365	60020301	ID=MS.gene63324.t1.exon3;Parent=MS.gene63324.t1
chr3.3	CDS	60020301	60020365	60020301	ID=cds.MS.gene63324.t1;Parent=MS.gene63324.t1
chr3.3	exon	60020528	60020684	60020528	ID=MS.gene63324.t1.exon4;Parent=MS.gene63324.t1
chr3.3	CDS	60020528	60020684	60020528	ID=cds.MS.gene63324.t1;Parent=MS.gene63324.t1
chr3.3	exon	60021118	60021255	60021118	ID=MS.gene63324.t1.exon5;Parent=MS.gene63324.t1
chr3.3	CDS	60021118	60021255	60021118	ID=cds.MS.gene63324.t1;Parent=MS.gene63324.t1

Gene Sequence

>MS.gene63324

ATGAAGAAGCAAGAGGTTGGAAAGAACAATGATGAAATGCAAAATTCTGAAGGACAAAATCATCATATGGTATGTATTGTTGACCATGGAATAAACAGTTACATAATAATTTCTGTAATTGAATTCATTTATTTTGTTGCTAAAATGTGATTTCATGCTTCCTCTGATGTTACTTATTGTCCATGCAGAATGCAATGAAAAATATATCTCTTCAGATCGCTGCTCTGGATAACCTAAATGCAGATGAAGAATTCGCAAATATGGTTGAAAATCATAAGGTTTATATTCCCTTTCCTTTTGTTTTGTTACTGATCAAAATTGTGTTTGTTAGGTTAACATTAGCATAATATTTATGTCATTTGAATATCAATTTATATACAGGGAGAAAAGGAACAACTTCATGTTGAATTCGTTAAGCTTGTAAGACAACTGGATATGAAACAAAAGTTGGAATGGGAGATTGGGCAACTAAAAGGATCATTAACTGTGTTGAATAATTCAAGTTGGATCATATTCATCCCATCAATGGGACAACTCCACAAAAAATGAGCCTCATTCTTCTTTCGCAACTGACTCAGCTTATTCCAATGGTTCTTCTCGATGTCTAGTGCATCGTTCATACAAGTCAGCAGAGCATTAAGCAATAAAAGCAACCTGCAAGTGACTTTCTCGAAGCGCCGCAGTGGGCTCTTCACGAAAGCTAGCAAGCTTTGCACCCTCTGTGGTGCATATGTTGCTCTAATTATATTCTCACCTGGTGAGAAGGTATTTTCATTTGGTCAACCAAGTGTTGAAACGGTCATAAATTGTTATTTCTCTCAGTTTCCACCCTAAAACAATGGCGTGATGCAATTCATTGAGGCATAACGTATCACCAAAGTGCGCAAGCTCGTTTTTGGTGGAGTTGTCCCATTGATGGGTCGAATATGATCACACTTGAATTATTCAAGAAGATTCTTGAATAATTCAAAAGTTGGATCATATTCATCATACCAATGGGAAGAGTCTACAAAAAATGAATTGAGCTTGCCCACTTTTGGCGCTACGGTATGCCTCAATGAATTGCATCATGCCATTGTTTTAGGGTGGAACCTGAGAGAGATAACGATTTATGACTGTTTCAACATTTGGCTGTCCAAATGAAATTACCTTCTCACCAGGTGAGAATATAATTAGAGCAACATATGCACCATAAAAGGGTGCAAAGCTCGCTAGCTTTCTTGAAGAGTCCACTACGACGCTTCGAGAAAGTCACTTGCAGGTTGCTTTCATTGCTCATCTTTTTCATCACAATCTTTTGGCGACCTCGAGTTTTCTTTCCGCTCGACATGGCTGA

Protein sequence

>MS.gene63324.t1

MKKQEVGKNNDEMQNSEGQNHHMNAMKNISLQIAALDNLNADEEFANMVENHKGEKEQLHVEFVKLVRQLDMKQNASFIQVSRALSNKSNLQVTFSKRRSGLFTKASKLCTLCGAYVALIIFSPGEKGAKLASFLEESTTTLRESHLQVAFIAHLFHHNLLATSSFLSARHG