AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63385


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63385.t1 MTR_2g075610 82.184 174 27 1 3 172 15 188 1.56e-101 291
MS.gene63385.t1 MTR_2g075750 81.609 174 23 2 3 172 14 182 5.63e-100 286
MS.gene63385.t1 MTR_2g075700 54.335 173 68 5 7 171 134 303 8.03e-56 179
MS.gene63385.t1 MTR_2g075690 48.315 178 75 5 3 167 127 300 2.21e-44 149
MS.gene63385.t1 MTR_2g075680 49.701 167 70 5 3 158 134 297 2.50e-44 149
MS.gene63385.t1 MTR_7g073430 52.846 123 55 3 7 127 112 233 2.51e-35 125
MS.gene63385.t1 MTR_7g073380 56.122 98 42 1 7 104 113 209 1.78e-34 123
MS.gene63385.t1 MTR_8g032510 47.183 142 69 5 7 145 108 246 2.04e-34 123
MS.gene63385.t1 MTR_0002s1250 45.217 115 60 3 7 119 101 214 2.83e-29 109
MS.gene63385.t1 MTR_8g005750 50.000 104 47 2 7 109 93 192 2.72e-28 107
MS.gene63385.t1 MTR_3g093830 50.000 68 34 0 13 80 123 190 4.39e-19 83.2
MS.gene63385.t1 MTR_8g099350 53.333 60 28 0 17 76 132 191 3.31e-18 81.3
MS.gene63385.t1 MTR_5g067700 39.286 112 58 3 3 110 93 198 4.88e-18 79.0
MS.gene63385.t1 MTR_7g117200 49.180 61 31 0 16 76 117 177 1.65e-16 76.3
MS.gene63385.t1 MTR_3g090860 37.500 96 60 0 16 111 132 227 1.87e-16 76.3
MS.gene63385.t1 MTR_5g067680 50.000 64 32 0 17 80 202 265 9.39e-16 74.3
MS.gene63385.t1 MTR_8g027860 46.875 64 34 0 16 79 135 198 3.88e-15 72.4
MS.gene63385.t1 MTR_4g094588 45.455 77 37 2 16 90 177 250 8.49e-15 71.6
MS.gene63385.t1 MTR_5g043880 34.483 116 73 1 16 131 123 235 1.49e-14 70.1
MS.gene63385.t1 MTR_7g079010 49.180 61 28 1 15 75 139 196 2.18e-14 68.9
MS.gene63385.t1 MTR_8g092140 48.333 60 28 1 15 74 158 214 2.34e-14 69.3
MS.gene63385.t1 MTR_4g082580 40.230 87 49 1 15 101 276 359 2.71e-14 70.5
MS.gene63385.t1 MTR_5g091390 47.761 67 30 2 16 80 156 219 1.18e-13 68.2
MS.gene63385.t1 MTR_3g095080 46.667 60 29 1 15 74 166 222 1.57e-13 67.0
MS.gene63385.t1 MTR_7g109800 50.847 59 26 1 16 74 144 199 1.93e-13 67.4
MS.gene63385.t1 MTR_3g095080 46.667 60 29 1 15 74 142 198 2.19e-13 66.2
MS.gene63385.t1 MTR_7g100510 43.023 86 45 2 11 96 356 437 2.76e-13 67.4
MS.gene63385.t1 MTR_5g042920 39.216 102 50 4 15 108 318 415 3.80e-13 67.0
MS.gene63385.t1 MTR_7g028415 46.667 60 29 1 15 74 91 147 5.23e-13 64.3
MS.gene63385.t1 MTR_7g028710 46.667 60 29 1 15 74 91 147 5.23e-13 64.3
MS.gene63385.t1 MTR_1g086790 49.153 59 27 1 16 74 157 212 6.62e-13 66.2
MS.gene63385.t1 MTR_2g105060 42.029 69 38 1 7 75 84 150 1.11e-12 64.7
MS.gene63385.t1 MTR_2g105060 42.029 69 38 1 7 75 159 225 1.36e-12 65.1
MS.gene63385.t1 MTR_8g007270 45.902 61 30 1 15 75 152 209 1.89e-12 63.9
MS.gene63385.t1 MTR_4g122530 45.763 59 29 1 16 74 207 262 3.45e-12 64.3
MS.gene63385.t1 MTR_5g016610 43.056 72 39 1 18 89 200 269 6.60e-12 62.8
MS.gene63385.t1 MTR_7g011820 45.902 61 30 1 15 75 408 465 7.86e-12 63.5
MS.gene63385.t1 MTR_5g073620 43.750 64 36 0 17 80 672 735 7.90e-12 63.5
MS.gene63385.t1 MTR_3g095040 41.333 75 40 2 2 75 102 173 8.14e-12 61.6
MS.gene63385.t1 MTR_5g094430 44.262 61 31 1 15 75 362 419 1.86e-11 62.4
MS.gene63385.t1 MTR_2g033820 45.763 59 29 1 16 74 202 257 2.24e-11 62.0
MS.gene63385.t1 MTR_3g056100 44.262 61 31 1 15 75 400 457 2.35e-11 62.0
MS.gene63385.t1 MTR_3g056100 44.262 61 31 1 15 75 413 470 2.53e-11 62.0
MS.gene63385.t1 MTR_5g094430 44.262 61 31 1 15 75 250 307 3.26e-11 61.6
MS.gene63385.t1 MTR_5g094430 44.262 61 31 1 15 75 228 285 3.37e-11 61.6
MS.gene63385.t1 MTR_7g062220 50.000 60 27 1 15 74 122 178 3.59e-11 61.2
MS.gene63385.t1 MTR_1g013760 42.029 69 38 1 7 75 130 196 4.07e-11 60.8
MS.gene63385.t1 MTR_4g127040 39.726 73 42 1 18 90 246 316 4.67e-11 60.8
MS.gene63385.t1 MTR_3g056100 44.262 61 31 1 15 75 256 313 4.74e-11 61.2
MS.gene63385.t1 MTR_3g056100 44.262 61 31 1 15 75 269 326 4.98e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63385.t1 AT5G22570 58.904 73 29 1 7 78 99 171 6.93e-24 95.9
MS.gene63385.t1 AT5G01900 54.545 66 30 0 12 77 105 170 2.66e-21 88.2
MS.gene63385.t1 AT1G66560 52.941 68 32 0 17 84 103 170 2.89e-21 87.8
MS.gene63385.t1 AT1G66600 55.556 63 28 0 16 78 102 164 8.36e-21 86.7
MS.gene63385.t1 AT1G66550 50.746 67 33 0 18 84 104 170 3.50e-20 85.1
MS.gene63385.t1 AT1G66550 50.746 67 33 0 18 84 109 175 4.59e-20 84.7
MS.gene63385.t1 AT3G56400 46.269 67 36 0 13 79 116 182 2.83e-19 83.6
MS.gene63385.t1 AT1G80590 52.459 61 29 0 18 78 86 146 4.37e-19 82.0
MS.gene63385.t1 AT5G24110 46.341 82 39 2 16 93 112 192 2.28e-17 78.2
MS.gene63385.t1 AT4G11070 33.333 129 75 4 17 135 109 236 4.25e-16 74.7
MS.gene63385.t1 AT4G11070 32.812 128 77 3 17 135 141 268 6.13e-16 74.7
MS.gene63385.t1 AT2G40750 46.667 60 32 0 17 76 152 211 2.99e-15 72.8
MS.gene63385.t1 AT2G46400 47.541 61 32 0 16 76 103 163 3.38e-15 72.4
MS.gene63385.t1 AT2G40740 45.000 60 33 0 17 76 173 232 4.77e-15 72.0
MS.gene63385.t1 AT4G23810 43.284 67 38 0 17 83 158 224 4.98e-15 72.0
MS.gene63385.t1 AT2G40740 45.000 60 33 0 17 76 182 241 5.82e-15 71.6
MS.gene63385.t1 AT1G64000 49.180 61 28 1 15 75 112 169 1.66e-14 68.9
MS.gene63385.t1 AT2G37260 43.617 94 39 4 16 109 348 427 6.05e-14 69.3
MS.gene63385.t1 AT2G37260 43.617 94 39 4 16 109 266 345 6.74e-14 68.9
MS.gene63385.t1 AT2G37260 43.617 94 39 4 16 109 268 347 6.80e-14 68.9
MS.gene63385.t1 AT4G18170 50.000 60 27 1 16 75 171 227 1.89e-13 67.8
MS.gene63385.t1 AT5G41570 47.541 61 29 1 15 75 96 153 2.05e-13 65.5
MS.gene63385.t1 AT1G29860 50.000 60 27 1 16 75 135 191 2.27e-13 67.0
MS.gene63385.t1 AT1G69310 47.458 59 28 1 16 74 146 201 3.18e-13 66.6
MS.gene63385.t1 AT1G69310 47.458 59 28 1 16 74 146 201 3.18e-13 66.6
MS.gene63385.t1 AT1G69310 47.458 59 28 1 16 74 146 201 3.18e-13 66.6
MS.gene63385.t1 AT2G03340 41.558 77 42 1 15 91 413 486 3.23e-13 67.4
MS.gene63385.t1 AT4G31800 43.478 69 37 1 7 75 101 167 3.82e-13 65.9
MS.gene63385.t1 AT2G46130 43.939 66 34 1 10 75 23 85 5.43e-13 62.8
MS.gene63385.t1 AT4G31800 44.615 65 34 1 11 75 170 232 6.40e-13 66.2
MS.gene63385.t1 AT4G39410 45.000 60 30 1 15 74 221 277 8.63e-13 65.9
MS.gene63385.t1 AT5G46350 48.333 60 28 1 16 75 182 238 9.53e-13 65.9
MS.gene63385.t1 AT2G44745 45.902 61 30 1 15 75 143 200 1.17e-12 64.3
MS.gene63385.t1 AT1G13960 47.541 61 29 1 15 75 407 464 1.35e-12 65.9
MS.gene63385.t1 AT5G13080 47.541 61 29 1 15 75 65 122 1.46e-12 62.8
MS.gene63385.t1 AT5G49520 47.458 59 28 1 16 74 220 275 1.55e-12 65.5
MS.gene63385.t1 AT2G25000 44.615 65 34 1 11 75 97 159 2.56e-12 63.5
MS.gene63385.t1 AT2G25000 44.615 65 34 1 11 75 97 159 2.56e-12 63.5
MS.gene63385.t1 AT2G47260 43.662 71 37 1 16 86 173 240 3.03e-12 64.3
MS.gene63385.t1 AT2G25000 44.615 65 34 1 11 75 119 181 3.13e-12 63.5
MS.gene63385.t1 AT1G13960 47.541 61 29 1 15 75 254 311 3.89e-12 63.9
MS.gene63385.t1 AT2G25000 44.615 65 34 1 11 75 140 202 4.42e-12 63.5
MS.gene63385.t1 AT5G26170 40.984 61 33 1 15 75 111 168 6.50e-12 61.6
MS.gene63385.t1 AT2G04880 47.541 61 29 1 15 75 281 338 8.07e-12 63.5
MS.gene63385.t1 AT2G04880 47.541 61 29 1 15 75 305 362 9.48e-12 63.2
MS.gene63385.t1 AT5G45260 44.828 58 32 0 18 75 1209 1266 1.64e-11 62.8
MS.gene63385.t1 AT2G30590 50.000 58 27 1 18 75 314 369 6.22e-11 60.8

Find 34 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTCAGATATCACATTAT+TGG 0.160744 2.4:+21757719 MS.gene63385:CDS
TAGGGTATTGTTCTTTGATT+TGG 0.255964 2.4:-21757788 None:intergenic
GGTAATCTGATGTTATTAAT+TGG 0.288389 2.4:-21757881 None:intergenic
TAATATATGGTTCTGTATAA+TGG 0.331566 2.4:-21757666 None:intergenic
GGCTATGAATGGAGAAAGTA+TGG 0.339396 2.4:+21757409 MS.gene63385:CDS
ATTATGATGGCCATAATATA+TGG 0.342578 2.4:-21757679 None:intergenic
TTATACAGAACCATATATTA+TGG 0.345556 2.4:+21757669 MS.gene63385:CDS
TTCAATTTCATGATGGTTGT+GGG 0.364141 2.4:+21757976 MS.gene63385:CDS
CTTCAATTTCATGATGGTTG+TGG 0.366368 2.4:+21757975 MS.gene63385:CDS
GGTATTGTTCTTTGATTTGG+AGG 0.380524 2.4:-21757785 None:intergenic
GTGACCAATGTGGTGATCAT+AGG 0.391548 2.4:-21757856 None:intergenic
AACATAGAAGAAGTACTATT+AGG 0.393005 2.4:-21757744 None:intergenic
TAAGATCAAAAGCACTTACT+TGG 0.396882 2.4:+21757356 MS.gene63385:intron
AACAAATACTGTTGATCCTA+TGG 0.399169 2.4:+21758010 MS.gene63385:CDS
AAGATGAATTTGATTATTTG+AGG 0.408137 2.4:+21757922 MS.gene63385:CDS
GATGGTTGTGGGCGCAATCT+TGG 0.419474 2.4:+21757987 MS.gene63385:CDS
TAGGGATTAATCACTTCTAT+AGG 0.427943 2.4:-21757837 None:intergenic
GATATCTGAATTTAATAAAG+AGG 0.448286 2.4:-21757709 None:intergenic
CAATCTCTTCAATTTCATGA+TGG 0.464117 2.4:+21757969 MS.gene63385:CDS
GGAGGCTGTCATTGAAGCTA+AGG 0.512687 2.4:-21757767 None:intergenic
TGATTGAAGATGGCTATGAA+TGG 0.519157 2.4:+21757398 MS.gene63385:CDS
TAATCAATGCAAAATACCTC+AGG 0.531627 2.4:+21757443 MS.gene63385:CDS
TGACCAATGTGGTGATCATA+GGG 0.567460 2.4:-21757855 None:intergenic
AAGATCAAAAGCACTTACTT+GGG 0.571753 2.4:+21757357 MS.gene63385:intron
AAAAGCACTTACTTGGGAGA+AGG 0.572656 2.4:+21757363 MS.gene63385:CDS
TATATGGTTCTGTATAATGG+AGG 0.587652 2.4:-21757663 None:intergenic
CTTACCTAATTAAAATCCAT+AGG 0.595764 2.4:-21758026 None:intergenic
ATATGGTTCTGTATAATGGA+GGG 0.605962 2.4:-21757662 None:intergenic
ATGAAATTGAAGAGATTGTG+TGG 0.612061 2.4:-21757964 None:intergenic
CTTCATAATCACATGAGAGA+GGG 0.620688 2.4:-21757902 None:intergenic
ACATGTGCAGAGAATTCAAG+AGG 0.632753 2.4:+21757638 MS.gene63385:CDS
TCTTCATAATCACATGAGAG+AGG 0.633424 2.4:-21757903 None:intergenic
AATCCCTATGATCACCACAT+TGG 0.633844 2.4:+21757852 MS.gene63385:CDS
TTAATTGGCTGTGACCAATG+TGG 0.819551 2.4:-21757866 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAAGAAATATGATATTTAT+TGG + chr2.4:21757315-21757334 MS.gene63385:intron 15.0%
!! AAATTCAGATATCACATTAT+TGG + chr2.4:21757719-21757738 MS.gene63385:CDS 20.0%
!! AAGATGAATTTGATTATTTG+AGG + chr2.4:21757922-21757941 MS.gene63385:CDS 20.0%
!! CAATATATCATTCATATCAT+AGG + chr2.4:21757565-21757584 MS.gene63385:intron 20.0%
!! GATATCTGAATTTAATAAAG+AGG - chr2.4:21757712-21757731 None:intergenic 20.0%
!! TTATACAGAACCATATATTA+TGG + chr2.4:21757669-21757688 MS.gene63385:CDS 20.0%
!!! TAATATATGGTTCTGTATAA+TGG - chr2.4:21757669-21757688 None:intergenic 20.0%
! ATTATGATGGCCATAATATA+TGG - chr2.4:21757682-21757701 None:intergenic 25.0%
! GGTAATCTGATGTTATTAAT+TGG - chr2.4:21757884-21757903 None:intergenic 25.0%
! TTGTGGATGATAAAAATGTA+GGG - chr2.4:21757809-21757828 None:intergenic 25.0%
! TTTGTGGATGATAAAAATGT+AGG - chr2.4:21757810-21757829 None:intergenic 25.0%
!! ATTGATGATATATATACCTG+AGG - chr2.4:21757462-21757481 None:intergenic 25.0%
!! ATTTTTATCATCCACAAAAC+TGG + chr2.4:21757812-21757831 MS.gene63385:CDS 25.0%
!!! AACATAGAAGAAGTACTATT+AGG - chr2.4:21757747-21757766 None:intergenic 25.0%
AACAAATACTGTTGATCCTA+TGG + chr2.4:21758010-21758029 MS.gene63385:CDS 30.0%
ATGAAATTGAAGAGATTGTG+TGG - chr2.4:21757967-21757986 None:intergenic 30.0%
CAATCTCTTCAATTTCATGA+TGG + chr2.4:21757969-21757988 MS.gene63385:CDS 30.0%
TAATCAATGCAAAATACCTC+AGG + chr2.4:21757443-21757462 MS.gene63385:CDS 30.0%
TAGGGATTAATCACTTCTAT+AGG - chr2.4:21757840-21757859 None:intergenic 30.0%
TTCAATTTCATGATGGTTGT+GGG + chr2.4:21757976-21757995 MS.gene63385:CDS 30.0%
! AAGAGGTTTTGCAATTATGA+TGG - chr2.4:21757695-21757714 None:intergenic 30.0%
! ATATGGTTCTGTATAATGGA+GGG - chr2.4:21757665-21757684 None:intergenic 30.0%
! TATATGGTTCTGTATAATGG+AGG - chr2.4:21757666-21757685 None:intergenic 30.0%
!! AAGATCAAAAGCACTTACTT+GGG + chr2.4:21757357-21757376 MS.gene63385:intron 30.0%
!! TAAGATCAAAAGCACTTACT+TGG + chr2.4:21757356-21757375 MS.gene63385:intron 30.0%
!! TAGGGTATTGTTCTTTGATT+TGG - chr2.4:21757791-21757810 None:intergenic 30.0%
!!! AGCTCAATTTTGATTGAAGA+TGG + chr2.4:21757388-21757407 MS.gene63385:CDS 30.0%
CTTCAATTTCATGATGGTTG+TGG + chr2.4:21757975-21757994 MS.gene63385:CDS 35.0%
CTTCATAATCACATGAGAGA+GGG - chr2.4:21757905-21757924 None:intergenic 35.0%
TCTTCATAATCACATGAGAG+AGG - chr2.4:21757906-21757925 None:intergenic 35.0%
TGATTGAAGATGGCTATGAA+TGG + chr2.4:21757398-21757417 MS.gene63385:CDS 35.0%
!! GGTATTGTTCTTTGATTTGG+AGG - chr2.4:21757788-21757807 None:intergenic 35.0%
AATCCCTATGATCACCACAT+TGG + chr2.4:21757852-21757871 MS.gene63385:CDS 40.0%
ACATGTGCAGAGAATTCAAG+AGG + chr2.4:21757638-21757657 MS.gene63385:CDS 40.0%
GGCTATGAATGGAGAAAGTA+TGG + chr2.4:21757409-21757428 MS.gene63385:CDS 40.0%
TGACCAATGTGGTGATCATA+GGG - chr2.4:21757858-21757877 None:intergenic 40.0%
TTAATTGGCTGTGACCAATG+TGG - chr2.4:21757869-21757888 None:intergenic 40.0%
! TTCTATAGGCTCCAGTTTTG+TGG - chr2.4:21757826-21757845 None:intergenic 40.0%
!! AAAAGCACTTACTTGGGAGA+AGG + chr2.4:21757363-21757382 MS.gene63385:CDS 40.0%
GTGACCAATGTGGTGATCAT+AGG - chr2.4:21757859-21757878 None:intergenic 45.0%
GGAGGCTGTCATTGAAGCTA+AGG - chr2.4:21757770-21757789 None:intergenic 50.0%
GATGGTTGTGGGCGCAATCT+TGG + chr2.4:21757987-21758006 MS.gene63385:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 21757251 21758043 21757251 ID=MS.gene63385
chr2.4 mRNA 21757251 21758043 21757251 ID=MS.gene63385.t1;Parent=MS.gene63385
chr2.4 exon 21757251 21757255 21757251 ID=MS.gene63385.t1.exon1;Parent=MS.gene63385.t1
chr2.4 CDS 21757251 21757255 21757251 ID=cds.MS.gene63385.t1;Parent=MS.gene63385.t1
chr2.4 exon 21757360 21757464 21757360 ID=MS.gene63385.t1.exon2;Parent=MS.gene63385.t1
chr2.4 CDS 21757360 21757464 21757360 ID=cds.MS.gene63385.t1;Parent=MS.gene63385.t1
chr2.4 exon 21757587 21758043 21757587 ID=MS.gene63385.t1.exon3;Parent=MS.gene63385.t1
chr2.4 CDS 21757587 21758043 21757587 ID=cds.MS.gene63385.t1;Parent=MS.gene63385.t1
Gene Sequence

>MS.gene63385

ATGATGTAATGCTATTGATTTCGTAAAACATTTATTTAGCTTAGCAGATTATGAGTTAATTGTACAAAGAAATATGATATTTATTGGTAGACTATTTCATTTTGATAAGATCAAAAGCACTTACTTGGGAGAAGGATAGCTCAATTTTGATTGAAGATGGCTATGAATGGAGAAAGTATGGACAAAAGAAGATAATCAATGCAAAATACCTCAGGTATATATATCATCAATTTCATCTTATAATAACTTACTCAAAATCTCTTTTTTTCTTCTTCTTCTATGACATTGTTAATTTTTAATGAATAATCATAATTCAATATATCATTCATATCATAGGAGCTACTATAGATGCAGTTACAAAAGTGATGAAGAATGTGAAGCTATGAAACATGTGCAGAGAATTCAAGAGGACCCTCCATTATACAGAACCATATATTATGGCCATCATAATTGCAAAACCTCTTTATTAAATTCAGATATCACATTATTGGAACCTAATAGTACTTCTTCTATGTTCCTTAGCTTCAATGACAGCCTCCAAATCAAAGAACAATACCCTACATTTTTATCATCCACAAAACTGGAGCCTATAGAAGTGATTAATCCCTATGATCACCACATTGGTCACAGCCAATTAATAACATCAGATTACCCTCTCTCATGTGATTATGAAGATGAATTTGATTATTTGAGGCATGCTACTATGTTATCATCCACACAATCTCTTCAATTTCATGATGGTTGTGGGCGCAATCTTGGAACAAATACTGTTGATCCTATGGATTTTAATTAG

Protein sequence

>MS.gene63385.t1

MISKALTWEKDSSILIEDGYEWRKYGQKKIINAKYLRSYYRCSYKSDEECEAMKHVQRIQEDPPLYRTIYYGHHNCKTSLLNSDITLLEPNSTSSMFLSFNDSLQIKEQYPTFLSSTKLEPIEVINPYDHHIGHSQLITSDYPLSCDYEDEFDYLRHATMLSSTQSLQFHDGCGRNLGTNTVDPMDFN