AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene63387

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63387.t1 MTR_2g075680 91.262 309 22 2 1 307 1 306 0.0 544
MS.gene63387.t1 MTR_2g075690 79.421 311 49 4 1 307 1 300 9.17e-173 480
MS.gene63387.t1 MTR_2g075700 74.026 308 70 7 1 307 1 299 2.24e-146 414
MS.gene63387.t1 MTR_7g073430 38.846 260 120 9 12 269 10 232 1.18e-46 159
MS.gene63387.t1 MTR_7g073380 39.834 241 108 8 12 247 11 219 2.51e-46 158
MS.gene63387.t1 MTR_2g075610 46.629 178 85 5 133 306 11 182 5.40e-44 149
MS.gene63387.t1 MTR_2g075750 48.555 173 78 5 136 306 13 176 3.51e-43 146
MS.gene63387.t1 MTR_0002s1250 37.154 253 109 9 10 253 3 214 2.83e-41 144
MS.gene63387.t1 MTR_8g032510 38.525 244 110 8 11 247 4 214 4.42e-39 139
MS.gene63387.t1 MTR_8g005750 37.931 232 99 6 19 247 8 197 3.73e-38 136
MS.gene63387.t1 MTR_3g093830 34.562 217 105 8 62 254 47 250 4.29e-26 105
MS.gene63387.t1 MTR_5g067700 30.894 246 123 8 7 251 5 204 3.51e-24 98.6
MS.gene63387.t1 MTR_8g099350 29.858 211 113 4 10 210 6 191 1.16e-23 99.8
MS.gene63387.t1 MTR_7g117200 29.646 226 104 6 9 221 5 188 2.52e-20 89.7
MS.gene63387.t1 MTR_3g090860 29.767 215 106 7 14 210 5 192 2.50e-18 84.3
MS.gene63387.t1 MTR_8g027860 44.211 95 44 2 127 213 105 198 3.22e-18 84.3
MS.gene63387.t1 MTR_5g067680 53.968 63 29 0 151 213 202 264 3.77e-16 78.2
MS.gene63387.t1 MTR_7g079010 43.137 102 41 4 109 208 109 195 2.00e-15 74.3
MS.gene63387.t1 MTR_7g028415 46.341 82 40 2 144 225 86 163 3.53e-15 72.4
MS.gene63387.t1 MTR_7g028710 46.341 82 40 2 144 225 86 163 3.53e-15 72.4
MS.gene63387.t1 MTR_2g075640 42.017 119 50 7 8 123 6 108 3.32e-14 68.2
MS.gene63387.t1 MTR_2g075730 42.017 119 50 7 8 123 6 108 4.27e-14 68.2
MS.gene63387.t1 MTR_7g109800 40.000 90 46 2 120 209 119 200 1.93e-13 69.7
MS.gene63387.t1 MTR_8g092140 48.333 60 28 1 149 208 158 214 2.22e-13 68.9
MS.gene63387.t1 MTR_5g085600 78.947 38 8 0 93 130 15 52 2.77e-13 64.3
MS.gene63387.t1 MTR_3g095080 47.541 61 29 1 149 209 166 223 3.63e-13 68.2
MS.gene63387.t1 MTR_8g007270 30.597 134 81 4 78 208 84 208 4.46e-13 67.8
MS.gene63387.t1 MTR_3g095080 47.541 61 29 1 149 209 142 199 4.49e-13 67.8
MS.gene63387.t1 MTR_1g086790 39.773 88 50 1 122 209 129 213 6.91e-13 68.6
MS.gene63387.t1 MTR_5g073620 31.081 148 81 4 93 224 599 741 1.59e-12 68.6
MS.gene63387.t1 MTR_4g122530 49.153 59 27 1 150 208 207 262 2.93e-12 67.0
MS.gene63387.t1 MTR_5g091390 50.847 59 26 1 150 208 156 211 4.07e-12 66.2
MS.gene63387.t1 MTR_7g100510 48.387 62 29 1 147 208 357 415 4.96e-12 66.6
MS.gene63387.t1 MTR_5g043880 41.250 80 41 2 150 226 123 199 5.41e-12 65.5
MS.gene63387.t1 MTR_5g042920 40.909 88 46 3 124 208 290 374 6.65e-12 66.2
MS.gene63387.t1 MTR_3g104750 43.662 71 37 1 144 214 97 164 8.13e-12 62.8
MS.gene63387.t1 MTR_4g082580 40.964 83 44 2 128 208 253 332 9.50e-12 65.5
MS.gene63387.t1 MTR_8g096730 45.000 60 30 1 149 208 68 124 9.57e-12 62.4
MS.gene63387.t1 MTR_2g033820 44.595 74 38 1 150 223 202 272 1.06e-11 65.5
MS.gene63387.t1 MTR_7g011820 46.667 60 29 1 149 208 408 464 1.26e-11 65.5
MS.gene63387.t1 MTR_4g094588 49.153 59 27 1 150 208 177 232 1.47e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene63387.t1 AT5G01900 31.500 200 101 5 13 211 6 170 1.16e-23 97.8
MS.gene63387.t1 AT5G22570 36.095 169 94 4 86 249 40 199 2.98e-23 97.4
MS.gene63387.t1 AT3G56400 32.381 210 105 7 7 210 1 179 3.73e-23 97.1
MS.gene63387.t1 AT1G66560 34.682 173 99 2 79 246 26 189 3.51e-22 93.6
MS.gene63387.t1 AT1G66600 60.317 63 25 0 150 212 102 164 7.76e-21 89.7
MS.gene63387.t1 AT1G66550 47.191 89 38 1 151 239 103 182 3.95e-20 87.8
MS.gene63387.t1 AT1G66550 47.191 89 38 1 151 239 108 187 5.33e-20 87.8
MS.gene63387.t1 AT4G11070 30.625 160 98 3 61 210 44 200 1.62e-19 87.4
MS.gene63387.t1 AT2G46400 57.377 61 26 0 150 210 103 163 1.14e-18 84.7
MS.gene63387.t1 AT1G80590 50.769 65 32 0 152 216 86 150 4.08e-18 82.0
MS.gene63387.t1 AT4G11070 30.622 209 92 7 10 210 5 168 4.48e-18 82.8
MS.gene63387.t1 AT2G40740 53.968 63 29 0 151 213 173 235 1.55e-17 81.6
MS.gene63387.t1 AT4G23810 37.681 138 67 5 86 213 92 220 2.21e-17 81.6
MS.gene63387.t1 AT2G40740 53.968 63 29 0 151 213 182 244 2.33e-17 81.3
MS.gene63387.t1 AT5G24110 31.410 156 75 3 63 210 41 172 1.44e-16 79.0
MS.gene63387.t1 AT2G46130 48.193 83 30 3 127 208 14 84 3.67e-15 70.9
MS.gene63387.t1 AT2G40750 28.708 209 133 6 13 212 12 213 8.40e-15 74.3
MS.gene63387.t1 AT5G49520 51.667 60 26 1 150 209 220 276 3.46e-13 70.1
MS.gene63387.t1 AT4G39410 41.758 91 47 3 130 217 199 286 4.68e-13 68.9
MS.gene63387.t1 AT1G64000 50.000 60 27 1 149 208 112 168 4.69e-13 67.0
MS.gene63387.t1 AT2G37260 36.364 121 64 3 98 208 286 403 5.40e-13 69.3
MS.gene63387.t1 AT2G44745 42.683 82 41 2 130 208 121 199 5.76e-13 67.4
MS.gene63387.t1 AT4G18170 40.230 87 49 1 122 208 143 226 6.22e-13 68.6
MS.gene63387.t1 AT2G37260 36.364 121 64 3 98 208 204 321 6.39e-13 68.9
MS.gene63387.t1 AT2G37260 36.364 121 64 3 98 208 206 323 6.52e-13 68.9
MS.gene63387.t1 AT5G41570 34.234 111 58 4 102 208 53 152 1.84e-12 65.1
MS.gene63387.t1 AT1G69310 47.458 59 28 1 150 208 146 201 4.61e-12 65.9
MS.gene63387.t1 AT1G69310 47.458 59 28 1 150 208 146 201 4.61e-12 65.9
MS.gene63387.t1 AT1G69310 47.458 59 28 1 150 208 146 201 4.61e-12 65.9
MS.gene63387.t1 AT2G47260 38.636 88 51 1 122 209 145 229 4.65e-12 66.2
MS.gene63387.t1 AT1G29860 50.847 59 26 1 150 208 135 190 5.29e-12 65.5
MS.gene63387.t1 AT1G13960 48.333 60 28 1 149 208 407 463 5.89e-12 66.6
MS.gene63387.t1 AT5G13080 47.541 61 29 1 149 209 65 122 1.15e-11 62.0
MS.gene63387.t1 AT5G46350 46.154 65 32 1 144 208 176 237 1.16e-11 65.1
MS.gene63387.t1 AT2G03340 48.333 60 28 1 149 208 413 469 1.34e-11 65.5
MS.gene63387.t1 AT1G13960 48.333 60 28 1 149 208 254 310 1.58e-11 64.7
MS.gene63387.t1 AT2G04880 44.262 61 30 2 148 208 108 164 2.93e-11 64.3
MS.gene63387.t1 AT2G04880 44.262 61 30 2 148 208 108 164 3.26e-11 64.3

Find 54 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGGCTTCCAACAAATTA+TGG 0.175004 2.4:-21735248 MS.gene63387:CDS
TTACCATTCAGACTTAAATT+TGG 0.184409 2.4:-21733370 MS.gene63387:CDS
ATGACCATCATCAATTAATT+TGG 0.194314 2.4:+21733725 None:intergenic
CCCTAAGATGGAGGAGGATT+TGG 0.206230 2.4:-21734940 MS.gene63387:CDS
CCCAAATCCTCCTCCATCTT+AGG 0.208617 2.4:+21734939 None:intergenic
AGATATATACCTGAAATATT+TGG 0.232157 2.4:+21733665 None:intergenic
CTAATAGTTGTAGGAGTTAT+AGG 0.241317 2.4:+21734987 None:intergenic
GTCAATATCAGAAGAGAAAA+TGG 0.250176 2.4:+21733344 None:intergenic
TCTTCCTTGGGATCTCCTTT+TGG 0.250749 2.4:+21733234 None:intergenic
TTTCTCTTGTTCGACACTTT+TGG 0.252228 2.4:+21734876 None:intergenic
ATCTTCAGCAACAATATTAA+AGG 0.276832 2.4:+21735091 None:intergenic
TCACAAATGCCAAATATTTC+AGG 0.281113 2.4:-21733674 MS.gene63387:intron
TCAAATATTAGAGAAGTTAA+AGG 0.289246 2.4:-21735143 MS.gene63387:CDS
AAACATCAAAATCGCATAAC+TGG 0.325641 2.4:+21733160 None:intergenic
TCCAACAAATTATGGCAAAA+AGG 0.332872 2.4:-21735240 MS.gene63387:CDS
TTGTACCGGACTACATATTA+TGG 0.352265 2.4:-21733414 MS.gene63387:CDS
CCTAAGATGGAGGAGGATTT+GGG 0.365034 2.4:-21734939 MS.gene63387:CDS
GATTCTCCGGTGAGAACATT+TGG 0.365162 2.4:+21733184 None:intergenic
ATCATGATGTATCTCTTCCT+TGG 0.417547 2.4:+21733221 None:intergenic
TTCAACTAATTGCCCTAAGA+TGG 0.421238 2.4:-21734952 MS.gene63387:CDS
AGGCAAAGAGTCCAATGATA+TGG 0.429516 2.4:-21735015 MS.gene63387:CDS
AGTAAACAAGAATATCCGTT+TGG 0.444637 2.4:-21733288 MS.gene63387:CDS
ATATAATGGAGAATATGCTT+TGG 0.459020 2.4:-21735265 None:intergenic
CCACTTCTTGCATCTCCAAA+AGG 0.459951 2.4:-21733249 MS.gene63387:CDS
CAAATATTAGAGAAGTTAAA+GGG 0.464900 2.4:-21735142 MS.gene63387:CDS
ATTCCAAATTTAAGTCTGAA+TGG 0.467467 2.4:+21733367 None:intergenic
TCTTCAGCAACAATATTAAA+GGG 0.477763 2.4:+21735092 None:intergenic
TATAGGAACAGCCATATCAT+TGG 0.478104 2.4:+21735004 None:intergenic
ACCATCATCAATTAATTTGG+AGG 0.482961 2.4:+21733728 None:intergenic
TCAGGTCAAGTGCACCAACT+TGG 0.484638 2.4:-21733761 MS.gene63387:intron
ATGTATGCAGGAGCTACTAT+AGG 0.489699 2.4:-21733506 MS.gene63387:intron
CCAAATCCTCCTCCATCTTA+GGG 0.502095 2.4:+21734940 None:intergenic
TCATGATGTATCTCTTCCTT+GGG 0.507868 2.4:+21733222 None:intergenic
GCCTCCAAATTAATTGATGA+TGG 0.512456 2.4:-21733729 MS.gene63387:CDS
TCGCATAACTGGAGATTCTC+CGG 0.520000 2.4:+21733171 None:intergenic
AAAAGAACCCTTCGTTGTAC+CGG 0.521119 2.4:-21733428 MS.gene63387:CDS
CAGGTCAAGTGCACCAACTT+GGG 0.523685 2.4:-21733760 MS.gene63387:CDS
GATGACCATAATATGTAGTC+CGG 0.554702 2.4:+21733409 None:intergenic
TAATTGATGATGGTCATACA+TGG 0.563264 2.4:-21733719 MS.gene63387:CDS
ATGTAGTCCGGTACAACGAA+GGG 0.569798 2.4:+21733421 None:intergenic
GGTCATACATGGAGAAAGTA+CGG 0.571382 2.4:-21733708 MS.gene63387:CDS
TGCACCAACTTGGGAAAAGA+CGG 0.575025 2.4:-21733751 MS.gene63387:CDS
AACTAATTGCCCTAAGATGG+AGG 0.587330 2.4:-21734949 MS.gene63387:CDS
ATCTCCAAAAGGAGATCCCA+AGG 0.601120 2.4:-21733238 MS.gene63387:CDS
TCAAAACCAAATGTTCTCAC+CGG 0.601930 2.4:-21733190 MS.gene63387:CDS
TGCTGAAGATCTTGTGCGTG+AGG 0.606211 2.4:-21735078 MS.gene63387:CDS
AATGGTGGTGACTGTCCAAA+CGG 0.615715 2.4:+21733273 None:intergenic
GGAGATGCAAGAAGTGGCAA+TGG 0.637841 2.4:+21733255 None:intergenic
GCTTATGAGAGGACGTGACA+TGG 0.640238 2.4:-21735204 MS.gene63387:CDS
TATGTAGTCCGGTACAACGA+AGG 0.640543 2.4:+21733420 None:intergenic
ATGGAAGATGAGCTTATGAG+AGG 0.648590 2.4:-21735215 MS.gene63387:CDS
GATGCAAGAAGTGGCAATGG+TGG 0.669301 2.4:+21733258 None:intergenic
TAATTGCCCTAAGATGGAGG+AGG 0.670680 2.4:-21734946 MS.gene63387:CDS
AAGATGGAGGAGGATTTGGG+TGG 0.700305 2.4:-21734936 MS.gene63387:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATGAAATTTAATATAGT+AGG + chr2.4:21733578-21733597 None:intergenic 10.0%
!! AATTAATATTATAAAGGAAA+GGG + chr2.4:21734329-21734348 None:intergenic 10.0%
!! ATATGAAATTTAATATAGTA+GGG + chr2.4:21733577-21733596 None:intergenic 10.0%
!! ATTAACTTTATAAGTATATA+TGG + chr2.4:21734273-21734292 None:intergenic 10.0%
!! TAATTAATATTATAAAGGAA+AGG + chr2.4:21734330-21734349 None:intergenic 10.0%
!! ATCAATCTTATAACTAAATT+TGG + chr2.4:21733702-21733721 None:intergenic 15.0%
!! TATAAATCATTCATTATATC+AGG - chr2.4:21734588-21734607 MS.gene63387:intron 15.0%
!! TTAAAATTTAATATGTATGC+AGG - chr2.4:21734849-21734868 MS.gene63387:intron 15.0%
!! TTTATAAGTATATATGGTTT+GGG + chr2.4:21734267-21734286 None:intergenic 15.0%
!! AATGAAAATAATTCATGCAA+TGG - chr2.4:21734146-21734165 MS.gene63387:intron 20.0%
!! AGATATATACCTGAAATATT+TGG + chr2.4:21734705-21734724 None:intergenic 20.0%
!! AGTTAATATAAATTGACCAT+AGG - chr2.4:21734286-21734305 MS.gene63387:intron 20.0%
!! ATGACATTCTATTATATTTG+GGG + chr2.4:21734050-21734069 None:intergenic 20.0%
!! CAAATATTAGAGAAGTTAAA+GGG - chr2.4:21733225-21733244 MS.gene63387:CDS 20.0%
!! CACAATATTAATAAAAACCT+AGG + chr2.4:21734093-21734112 None:intergenic 20.0%
!! CTTTATAAGTATATATGGTT+TGG + chr2.4:21734268-21734287 None:intergenic 20.0%
!! GATGACATTCTATTATATTT+GGG + chr2.4:21734051-21734070 None:intergenic 20.0%
!! TACTTACATAGTATAAAACA+TGG + chr2.4:21733531-21733550 None:intergenic 20.0%
!! TATTTGTATCGTTATGATAT+CGG - chr2.4:21733597-21733616 MS.gene63387:intron 20.0%
!! TCAAATATTAGAGAAGTTAA+AGG - chr2.4:21733224-21733243 MS.gene63387:CDS 20.0%
!!! ATTTGCTTTTTTTTTTTGTC+AGG + chr2.4:21734381-21734400 None:intergenic 20.0%
!!! GTTTAAAACTTTAGAACAAT+TGG + chr2.4:21733639-21733658 None:intergenic 20.0%
!!! TTTGCTTTTTTTTTTTGTCA+GGG + chr2.4:21734380-21734399 None:intergenic 20.0%
! AAAATAATTCATGCAATGGT+GGG - chr2.4:21734150-21734169 MS.gene63387:intron 25.0%
! ATACTCAATGGAAGAATATA+TGG - chr2.4:21734494-21734513 MS.gene63387:intron 25.0%
! ATCTTCAGCAACAATATTAA+AGG + chr2.4:21733279-21733298 None:intergenic 25.0%
! ATGACCATCATCAATTAATT+TGG + chr2.4:21734645-21734664 None:intergenic 25.0%
! ATGATGATCATATATATGTC+TGG + chr2.4:21734471-21734490 None:intergenic 25.0%
! ATTCCAAATTTAAGTCTGAA+TGG + chr2.4:21735003-21735022 None:intergenic 25.0%
! ATTTGAATGTTACAGATTGA+TGG + chr2.4:21734802-21734821 None:intergenic 25.0%
! GAAAAATCTCTAATAGTTGT+AGG + chr2.4:21733392-21733411 None:intergenic 25.0%
! GGATGACATTCTATTATATT+TGG + chr2.4:21734052-21734071 None:intergenic 25.0%
! TCTTCAGCAACAATATTAAA+GGG + chr2.4:21733278-21733297 None:intergenic 25.0%
! TTACCATTCAGACTTAAATT+TGG - chr2.4:21734997-21735016 MS.gene63387:CDS 25.0%
!!! AACTTTAGAACAATTGGAAA+AGG + chr2.4:21733633-21733652 None:intergenic 25.0%
!!! TTGCTTTTTTTTTTTGTCAG+GGG + chr2.4:21734379-21734398 None:intergenic 25.0%
AAACATCAAAATCGCATAAC+TGG + chr2.4:21735210-21735229 None:intergenic 30.0%
AAATCCATATACAACCAAGA+AGG - chr2.4:21733984-21734003 MS.gene63387:intron 30.0%
AAATTATGGCAAAAAGGCAA+TGG - chr2.4:21733133-21733152 MS.gene63387:CDS 30.0%
ACAAAAACCCTAAACAAAGA+AGG + chr2.4:21733959-21733978 None:intergenic 30.0%
AGTAAACAAGAATATCCGTT+TGG - chr2.4:21735079-21735098 MS.gene63387:CDS 30.0%
ATGATCATATATATGTCTGG+AGG + chr2.4:21734468-21734487 None:intergenic 30.0%
CTAATAGTTGTAGGAGTTAT+AGG + chr2.4:21733383-21733402 None:intergenic 30.0%
GAAAATAATTCATGCAATGG+TGG - chr2.4:21734149-21734168 MS.gene63387:intron 30.0%
GATCATCATTACATACTCAA+TGG - chr2.4:21734482-21734501 MS.gene63387:intron 30.0%
GTCAATATCAGAAGAGAAAA+TGG + chr2.4:21735026-21735045 None:intergenic 30.0%
TCACAAATGCCAAATATTTC+AGG - chr2.4:21734693-21734712 MS.gene63387:intron 30.0%
TCCAACAAATTATGGCAAAA+AGG - chr2.4:21733127-21733146 MS.gene63387:CDS 30.0%
TGGATAATTCATGTGAGATT+GGG - chr2.4:21734238-21734257 MS.gene63387:intron 30.0%
TTACTTAAAAGTTGTCTCCT+AGG - chr2.4:21734073-21734092 MS.gene63387:intron 30.0%
TTGAGATTTACTTAGACCTA+TGG + chr2.4:21734305-21734324 None:intergenic 30.0%
! ACCATCATCAATTAATTTGG+AGG + chr2.4:21734642-21734661 None:intergenic 30.0%
! TTCTCTTCCTTCTTTGTTTA+GGG - chr2.4:21733949-21733968 MS.gene63387:intron 30.0%
! TTTCTCTTCCTTCTTTGTTT+AGG - chr2.4:21733948-21733967 MS.gene63387:intron 30.0%
!! AGGAGACAACTTTTAAGTAA+GGG + chr2.4:21734073-21734092 None:intergenic 30.0%
!! TAATTGATGATGGTCATACA+TGG - chr2.4:21734648-21734667 MS.gene63387:intron 30.0%
!! TAGGAGACAACTTTTAAGTA+AGG + chr2.4:21734074-21734093 None:intergenic 30.0%
!! TTCTTTGTTTAGGGTTTTTG+TGG - chr2.4:21733958-21733977 MS.gene63387:intron 30.0%
!!! TTGTTTTTGTTTCTCTCTCT+GGG + chr2.4:21734027-21734046 None:intergenic 30.0%
ACCAAGAAGGAAGATCATTA+AGG - chr2.4:21733997-21734016 MS.gene63387:intron 35.0%
ATCATGATGTATCTCTTCCT+TGG + chr2.4:21735149-21735168 None:intergenic 35.0%
GATGACCATAATATGTAGTC+CGG + chr2.4:21734961-21734980 None:intergenic 35.0%
GCCTCCAAATTAATTGATGA+TGG - chr2.4:21734638-21734657 MS.gene63387:intron 35.0%
GTGGATAATTCATGTGAGAT+TGG - chr2.4:21734237-21734256 MS.gene63387:intron 35.0%
TATAGGAACAGCCATATCAT+TGG + chr2.4:21733366-21733385 None:intergenic 35.0%
TATCGTTATGATATCGGACA+AGG - chr2.4:21733603-21733622 MS.gene63387:intron 35.0%
TCAAAACCAAATGTTCTCAC+CGG - chr2.4:21735177-21735196 MS.gene63387:CDS 35.0%
TCATGATGTATCTCTTCCTT+GGG + chr2.4:21735148-21735167 None:intergenic 35.0%
TCCTTAATGATCTTCCTTCT+TGG + chr2.4:21734001-21734020 None:intergenic 35.0%
TCTTCCTTCTTGGTTGTATA+TGG + chr2.4:21733991-21734010 None:intergenic 35.0%
TTCAACTAATTGCCCTAAGA+TGG - chr2.4:21733415-21733434 MS.gene63387:CDS 35.0%
TTGTACCGGACTACATATTA+TGG - chr2.4:21734953-21734972 MS.gene63387:CDS 35.0%
! ACACTTTTGCTCTTGAACAA+AGG - chr2.4:21733332-21733351 MS.gene63387:CDS 35.0%
! CTTTGGCTTCCAACAAATTA+TGG - chr2.4:21733119-21733138 MS.gene63387:CDS 35.0%
! GTTCGACACTTTTGGATTTT+TGG + chr2.4:21733486-21733505 None:intergenic 35.0%
! TTTCTCTTGTTCGACACTTT+TGG + chr2.4:21733494-21733513 None:intergenic 35.0%
!! CTAGTTTTTGCAAAACCAGT+TGG + chr2.4:21733925-21733944 None:intergenic 35.0%
!! GCCTTTTTGCCATAATTTGT+TGG + chr2.4:21733131-21733150 None:intergenic 35.0%
!! GTCTTTTAAGAGTAGTGACA+TGG + chr2.4:21734405-21734424 None:intergenic 35.0%
!!! ACTGGTTTTGCAAAAACTAG+AGG - chr2.4:21733925-21733944 MS.gene63387:intron 35.0%
!!! CTTTTTTTTTTTGTCAGGGG+TGG + chr2.4:21734376-21734395 None:intergenic 35.0%
!!! GTTGTTTTTGTTTCTCTCTC+TGG + chr2.4:21734028-21734047 None:intergenic 35.0%
AAAAGAACCCTTCGTTGTAC+CGG - chr2.4:21734939-21734958 MS.gene63387:CDS 40.0%
AAAATGTGTGATGCACAGCT+AGG - chr2.4:21734442-21734461 MS.gene63387:intron 40.0%
AACTAATTGCCCTAAGATGG+AGG - chr2.4:21733418-21733437 MS.gene63387:CDS 40.0%
AGGCAAAGAGTCCAATGATA+TGG - chr2.4:21733352-21733371 MS.gene63387:CDS 40.0%
ATGGAAGATGAGCTTATGAG+AGG - chr2.4:21733152-21733171 MS.gene63387:CDS 40.0%
ATGTATGCAGGAGCTACTAT+AGG - chr2.4:21734861-21734880 MS.gene63387:intron 40.0%
GGTCATACATGGAGAAAGTA+CGG - chr2.4:21734659-21734678 MS.gene63387:intron 40.0%
TCAACTTTCAATCTCGAGTG+TGG - chr2.4:21734218-21734237 MS.gene63387:intron 40.0%
! GTTTTTGCAAAACCAGTTGG+CGG + chr2.4:21733922-21733941 None:intergenic 40.0%
!!! GGTGAGAACATTTGGTTTTG+AGG + chr2.4:21735178-21735197 None:intergenic 40.0%
!!! TTTTTTTTTGTCAGGGGTGG+AGG + chr2.4:21734373-21734392 None:intergenic 40.0%
ATCTCCAAAAGGAGATCCCA+AGG - chr2.4:21735129-21735148 MS.gene63387:CDS 45.0%
ATGTAGTCCGGTACAACGAA+GGG + chr2.4:21734949-21734968 None:intergenic 45.0%
CCAAATCCTCCTCCATCTTA+GGG + chr2.4:21733430-21733449 None:intergenic 45.0%
CCACTTCTTGCATCTCCAAA+AGG - chr2.4:21735118-21735137 MS.gene63387:CDS 45.0%
CCTAAGATGGAGGAGGATTT+GGG - chr2.4:21733428-21733447 MS.gene63387:CDS 45.0%
GAGGAGAGGGAGAAAAATGA+AGG + chr2.4:21733900-21733919 None:intergenic 45.0%
GATTCTCCGGTGAGAACATT+TGG + chr2.4:21735186-21735205 None:intergenic 45.0%
TAATTGCCCTAAGATGGAGG+AGG - chr2.4:21733421-21733440 MS.gene63387:CDS 45.0%
TATGTAGTCCGGTACAACGA+AGG + chr2.4:21734950-21734969 None:intergenic 45.0%
TCGCATAACTGGAGATTCTC+CGG + chr2.4:21735199-21735218 None:intergenic 45.0%
TGCACCAACTTGGGAAAAGA+CGG - chr2.4:21734616-21734635 MS.gene63387:intron 45.0%
! AATGGTGGTGACTGTCCAAA+CGG + chr2.4:21735097-21735116 None:intergenic 45.0%
! TCTTCCTTGGGATCTCCTTT+TGG + chr2.4:21735136-21735155 None:intergenic 45.0%
!! CCTTTTGGAGATGCAAGAAG+TGG + chr2.4:21735121-21735140 None:intergenic 45.0%
!! ATATATATATAGATATATAT+CGG + chr2.4:21733788-21733807 None:intergenic 5.0%
!! GAATTTAATTAATATTATAA+AGG + chr2.4:21734335-21734354 None:intergenic 5.0%
AAGATGGAGGAGGATTTGGG+TGG - chr2.4:21733431-21733450 MS.gene63387:CDS 50.0%
CAGGTCAAGTGCACCAACTT+GGG - chr2.4:21734607-21734626 MS.gene63387:intron 50.0%
CCCAAATCCTCCTCCATCTT+AGG + chr2.4:21733431-21733450 None:intergenic 50.0%
CCCTAAGATGGAGGAGGATT+TGG - chr2.4:21733427-21733446 MS.gene63387:CDS 50.0%
GATGCAAGAAGTGGCAATGG+TGG + chr2.4:21735112-21735131 None:intergenic 50.0%
GCTTATGAGAGGACGTGACA+TGG - chr2.4:21733163-21733182 MS.gene63387:CDS 50.0%
GGAGATGCAAGAAGTGGCAA+TGG + chr2.4:21735115-21735134 None:intergenic 50.0%
TCAGGTCAAGTGCACCAACT+TGG - chr2.4:21734606-21734625 MS.gene63387:intron 50.0%
TGCTGAAGATCTTGTGCGTG+AGG - chr2.4:21733289-21733308 MS.gene63387:CDS 50.0%
TTTGCAAAACCAGTTGGCGG+AGG + chr2.4:21733919-21733938 None:intergenic 50.0%
TTTGTCAGGGGTGGAGGATT+TGG + chr2.4:21734367-21734386 None:intergenic 50.0%
AAAACCAGTTGGCGGAGGAG+AGG + chr2.4:21733914-21733933 None:intergenic 55.0%
AAACCAGTTGGCGGAGGAGA+GGG + chr2.4:21733913-21733932 None:intergenic 55.0%
! GAGGCCGTCTTTTCCCAAGT+TGG + chr2.4:21734623-21734642 None:intergenic 55.0%
TCTCCCTCTCCTCCGCCAAC+TGG - chr2.4:21733907-21733926 MS.gene63387:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 21733107 21735282 21733107 ID=MS.gene63387
chr2.4 mRNA 21733107 21735282 21733107 ID=MS.gene63387.t1;Parent=MS.gene63387
chr2.4 exon 21734876 21735282 21734876 ID=MS.gene63387.t1.exon1;Parent=MS.gene63387.t1
chr2.4 CDS 21734876 21735282 21734876 ID=cds.MS.gene63387.t1;Parent=MS.gene63387.t1
chr2.4 exon 21733675 21733779 21733675 ID=MS.gene63387.t1.exon2;Parent=MS.gene63387.t1
chr2.4 CDS 21733675 21733779 21733675 ID=cds.MS.gene63387.t1;Parent=MS.gene63387.t1
chr2.4 exon 21733107 21733518 21733107 ID=MS.gene63387.t1.exon3;Parent=MS.gene63387.t1
chr2.4 CDS 21733107 21733518 21733107 ID=cds.MS.gene63387.t1;Parent=MS.gene63387.t1
Gene Sequence

>MS.gene63387

ATGGAGAATATGCTTTGGCTTCCAACAAATTATGGCAAAAAGGCAATGGAAGATGAGCTTATGAGAGGACGTGACATGGCAAATCAATTACTTGAAGTGCTAACTTTTGATGATAAGTCAAATATTAGAGAAGTTAAAGGGTCAAACACAAAATCATCAAAAGTGCTACCCTTTAATATTGTTGCTGAAGATCTTGTGCGTGAGGTTCTCAAATCATTGACAAATACACTTTTGCTCTTGAACAAAGGCAAAGAGTCCAATGATATGGCTGTTCCTATAACTCCTACAACTATTAGAGATTTTTCTTTTTCAACTAATTGCCCTAAGATGGAGGAGGATTTGGGTGGAGCTTACAAGAAACTCAAAACTCTCAACACCAAAAATCCAAAAGTGTCGAACAAGAGAAAGTAAGAATCTTAATCCATGTTTTATACTATGTAAGTATTTGACTTGTTTCAAGTTTTTTTCCCTACTATATTAAATTTCATATTATTTGTATCGTTATGATATCGGACAAGGACTTCCTTTTCCAATTGTTCTAAAGTTTTAAACATGAAGAAAACTTAGCTAGATTCAACAATAATCTTGATATCCAAATTTAGTTATAAGATTGATTTTTGAATTAACAGTCAATTATGTCATTAAACTAATAGAAAAGTTTGCAATTTCTCTCTTTCTCCGATATATATCTATATATATATATATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAAATATAATTTAGTACAACTTTATTTTCTAAATCCTTCATTTTTCTCCCTCTCCTCCGCCAACTGGTTTTGCAAAAACTAGAGGTTTCTCTTCCTTCTTTGTTTAGGGTTTTTGTGGCTCAAATCCATATACAACCAAGAAGGAAGATCATTAAGGAAAACCCAGAGAGAGAAACAAAAACAACCCCAAATATAATAGAATGTCATCCCTTACTTAAAAGTTGTCTCCTAGGTTTTTATTAATATTGTGTTATTTTTCTATAAAAATTATATTAATTCTTAAAATGAAAATAATTCATGCAATGGTGGGTATATATATTATATTTGTGTAATATTTTACTATCTATATATAATATCAACTTTCAATCTCGAGTGTGGATAATTCATGTGAGATTGGGTAACCCAAACCATATATACTTATAAAGTTAATATAAATTGACCATAGGTCTAAGTAAATCTCAAACCCTTTCCTTTATAATATTAATTAAATTCTTATATTGTCCAAATCCTCCACCCCTGACAAAAAAAAAAAGCAAATACCATGTCACTACTCTTAAAAGACAAGTTAACGAAATAACAAAAATGTGTGATGCACAGCTAGGCCTCCAGACATATATATGATCATCATTACATACTCAATGGAAGAATATATGGATCTATTAATATTTTATAAATATTTTCGTCAATTTGATTAGTTAATTTATGATACTTATGAAGTAATTGAATATAAATCATTCATTATATCAGGTCAAGTGCACCAACTTGGGAAAAGACGGCCTCCAAATTAATTGATGATGGTCATACATGGAGAAAGTACGGACAAAAAATGATCACAAATGCCAAATATTTCAGGTATATATCTATAGTGCAAATTCAAATAAGAATAAATATTAGTCAACATTATACACTAATGTGATAATTTTAATGTGTTAGATTCCATCAATCTGTAACATTCAAATTAAAAATTAATATAGATAAAGTTAAGTTTAAAATTTAATATGTATGCAGGAGCTACTATAGGTGCACTCACATGAATGATCAACAATGTGAAGCAATCAAACATGTTCAAAGAATACAAAAGAACCCTTCGTTGTACCGGACTACATATTATGGTCATCACACTTGCAAAAGCTCTTACCATTCAGACTTAAATTTGGAATCCATTTTCTCTTCTGATATTGACTCTTCAATATTACTTAGTTTCGATAACATTTCAAGTAAACAAGAATATCCGTTTGGACAGTCACCACCATTGCCACTTCTTGCATCTCCAAAAGGAGATCCCAAGGAAGAGATACATCATGATCATTTTCCTCAAAACCAAATGTTCTCACCGGAGAATCTCCAGTTATGCGATTTTGATGTTTATTTTGATTATTTGAGAGATGTCACTGAGTCATCATCGAGTGAATCTTTTTAA
Protein sequence

>MS.gene63387.t1

MENMLWLPTNYGKKAMEDELMRGRDMANQLLEVLTFDDKSNIREVKGSNTKSSKVLPFNIVAEDLVREVLKSLTNTLLLLNKGKESNDMAVPITPTTIRDFSFSTNCPKMEEDLGGAYKKLKTLNTKNPKVSNKRKSSAPTWEKTASKLIDDGHTWRKYGQKMITNAKYFRSYYRCTHMNDQQCEAIKHVQRIQKNPSLYRTTYYGHHTCKSSYHSDLNLESIFSSDIDSSILLSFDNISSKQEYPFGQSPPLPLLASPKGDPKEEIHHDHFPQNQMFSPENLQLCDFDVYFDYLRDVTESSSSESF