AlfalfaGEDB | MS.gene63387

M. sativa cultivar XinJiangDaYe / MS.gene63387

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043565 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03106.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63387.t1	XP_003596299.1	90.9	309	23	2	1	307	1	306	1.20E-133	486.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63387.t1	Q8GWF1	44.0	75	33	2	175	249	134	199	3.5e-09	63.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63387.t1	G7IR29	90.9	309	23	2	1	307	1	306	8.6e-134	486.1

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene63387.t1	TF	WRKY

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63387.t1	MTR_2g075680	91.262	309	22	2	1	307	1	306	0.0	544
MS.gene63387.t1	MTR_2g075690	79.421	311	49	4	1	307	1	300	9.17e-173	480
MS.gene63387.t1	MTR_2g075700	74.026	308	70	7	1	307	1	299	2.24e-146	414
MS.gene63387.t1	MTR_7g073430	38.846	260	120	9	12	269	10	232	1.18e-46	159
MS.gene63387.t1	MTR_7g073380	39.834	241	108	8	12	247	11	219	2.51e-46	158
MS.gene63387.t1	MTR_2g075610	46.629	178	85	5	133	306	11	182	5.40e-44	149
MS.gene63387.t1	MTR_2g075750	48.555	173	78	5	136	306	13	176	3.51e-43	146
MS.gene63387.t1	MTR_0002s1250	37.154	253	109	9	10	253	3	214	2.83e-41	144
MS.gene63387.t1	MTR_8g032510	38.525	244	110	8	11	247	4	214	4.42e-39	139
MS.gene63387.t1	MTR_8g005750	37.931	232	99	6	19	247	8	197	3.73e-38	136

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63387.t1	AT5G01900	31.500	200	101	5	13	211	6	170	1.16e-23	97.8
MS.gene63387.t1	AT5G22570	36.095	169	94	4	86	249	40	199	2.98e-23	97.4
MS.gene63387.t1	AT3G56400	32.381	210	105	7	7	210	1	179	3.73e-23	97.1
MS.gene63387.t1	AT1G66560	34.682	173	99	2	79	246	26	189	3.51e-22	93.6
MS.gene63387.t1	AT1G66600	60.317	63	25	0	150	212	102	164	7.76e-21	89.7
MS.gene63387.t1	AT1G66550	47.191	89	38	1	151	239	103	182	3.95e-20	87.8
MS.gene63387.t1	AT1G66550	47.191	89	38	1	151	239	108	187	5.33e-20	87.8
MS.gene63387.t1	AT4G11070	30.625	160	98	3	61	210	44	200	1.62e-19	87.4
MS.gene63387.t1	AT2G46400	57.377	61	26	0	150	210	103	163	1.14e-18	84.7
MS.gene63387.t1	AT1G80590	50.769	65	32	0	152	216	86	150	4.08e-18	82.0

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Find 54 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAACCCTTCGTTGTAC+CGG	0.521119	2.4:-21733428	MS.gene63387:CDS
AAACATCAAAATCGCATAAC+TGG	0.325641	2.4:+21733160	None:intergenic
AACTAATTGCCCTAAGATGG+AGG	0.587330	2.4:-21734949	MS.gene63387:CDS
AAGATGGAGGAGGATTTGGG+TGG	0.700305	2.4:-21734936	MS.gene63387:CDS
AATGGTGGTGACTGTCCAAA+CGG	0.615715	2.4:+21733273	None:intergenic
ACCATCATCAATTAATTTGG+AGG	0.482961	2.4:+21733728	None:intergenic
AGATATATACCTGAAATATT+TGG	0.232157	2.4:+21733665	None:intergenic
AGGCAAAGAGTCCAATGATA+TGG	0.429516	2.4:-21735015	MS.gene63387:CDS
AGTAAACAAGAATATCCGTT+TGG	0.444637	2.4:-21733288	MS.gene63387:CDS
ATATAATGGAGAATATGCTT+TGG	0.459020	2.4:-21735265	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACATCAAAATCGCATAAC+TGG	+	chr2.4:21735210-21735229	None:intergenic	30.0%
AAATCCATATACAACCAAGA+AGG	-	chr2.4:21733984-21734003	MS.gene63387:intron	30.0%
AAATTATGGCAAAAAGGCAA+TGG	-	chr2.4:21733133-21733152	MS.gene63387:CDS	30.0%
ACAAAAACCCTAAACAAAGA+AGG	+	chr2.4:21733959-21733978	None:intergenic	30.0%
AGTAAACAAGAATATCCGTT+TGG	-	chr2.4:21735079-21735098	MS.gene63387:CDS	30.0%
ATGATCATATATATGTCTGG+AGG	+	chr2.4:21734468-21734487	None:intergenic	30.0%
CTAATAGTTGTAGGAGTTAT+AGG	+	chr2.4:21733383-21733402	None:intergenic	30.0%
GAAAATAATTCATGCAATGG+TGG	-	chr2.4:21734149-21734168	MS.gene63387:intron	30.0%
GATCATCATTACATACTCAA+TGG	-	chr2.4:21734482-21734501	MS.gene63387:intron	30.0%
GTCAATATCAGAAGAGAAAA+TGG	+	chr2.4:21735026-21735045	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	21733107	21735282	21733107	ID=MS.gene63387
chr2.4	mRNA	21733107	21735282	21733107	ID=MS.gene63387.t1;Parent=MS.gene63387
chr2.4	exon	21734876	21735282	21734876	ID=MS.gene63387.t1.exon1;Parent=MS.gene63387.t1
chr2.4	CDS	21734876	21735282	21734876	ID=cds.MS.gene63387.t1;Parent=MS.gene63387.t1
chr2.4	exon	21733675	21733779	21733675	ID=MS.gene63387.t1.exon2;Parent=MS.gene63387.t1
chr2.4	CDS	21733675	21733779	21733675	ID=cds.MS.gene63387.t1;Parent=MS.gene63387.t1
chr2.4	exon	21733107	21733518	21733107	ID=MS.gene63387.t1.exon3;Parent=MS.gene63387.t1
chr2.4	CDS	21733107	21733518	21733107	ID=cds.MS.gene63387.t1;Parent=MS.gene63387.t1

Gene Sequence

>MS.gene63387

ATGGAGAATATGCTTTGGCTTCCAACAAATTATGGCAAAAAGGCAATGGAAGATGAGCTTATGAGAGGACGTGACATGGCAAATCAATTACTTGAAGTGCTAACTTTTGATGATAAGTCAAATATTAGAGAAGTTAAAGGGTCAAACACAAAATCATCAAAAGTGCTACCCTTTAATATTGTTGCTGAAGATCTTGTGCGTGAGGTTCTCAAATCATTGACAAATACACTTTTGCTCTTGAACAAAGGCAAAGAGTCCAATGATATGGCTGTTCCTATAACTCCTACAACTATTAGAGATTTTTCTTTTTCAACTAATTGCCCTAAGATGGAGGAGGATTTGGGTGGAGCTTACAAGAAACTCAAAACTCTCAACACCAAAAATCCAAAAGTGTCGAACAAGAGAAAGTAAGAATCTTAATCCATGTTTTATACTATGTAAGTATTTGACTTGTTTCAAGTTTTTTTCCCTACTATATTAAATTTCATATTATTTGTATCGTTATGATATCGGACAAGGACTTCCTTTTCCAATTGTTCTAAAGTTTTAAACATGAAGAAAACTTAGCTAGATTCAACAATAATCTTGATATCCAAATTTAGTTATAAGATTGATTTTTGAATTAACAGTCAATTATGTCATTAAACTAATAGAAAAGTTTGCAATTTCTCTCTTTCTCCGATATATATCTATATATATATATATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAAATATAATTTAGTACAACTTTATTTTCTAAATCCTTCATTTTTCTCCCTCTCCTCCGCCAACTGGTTTTGCAAAAACTAGAGGTTTCTCTTCCTTCTTTGTTTAGGGTTTTTGTGGCTCAAATCCATATACAACCAAGAAGGAAGATCATTAAGGAAAACCCAGAGAGAGAAACAAAAACAACCCCAAATATAATAGAATGTCATCCCTTACTTAAAAGTTGTCTCCTAGGTTTTTATTAATATTGTGTTATTTTTCTATAAAAATTATATTAATTCTTAAAATGAAAATAATTCATGCAATGGTGGGTATATATATTATATTTGTGTAATATTTTACTATCTATATATAATATCAACTTTCAATCTCGAGTGTGGATAATTCATGTGAGATTGGGTAACCCAAACCATATATACTTATAAAGTTAATATAAATTGACCATAGGTCTAAGTAAATCTCAAACCCTTTCCTTTATAATATTAATTAAATTCTTATATTGTCCAAATCCTCCACCCCTGACAAAAAAAAAAAGCAAATACCATGTCACTACTCTTAAAAGACAAGTTAACGAAATAACAAAAATGTGTGATGCACAGCTAGGCCTCCAGACATATATATGATCATCATTACATACTCAATGGAAGAATATATGGATCTATTAATATTTTATAAATATTTTCGTCAATTTGATTAGTTAATTTATGATACTTATGAAGTAATTGAATATAAATCATTCATTATATCAGGTCAAGTGCACCAACTTGGGAAAAGACGGCCTCCAAATTAATTGATGATGGTCATACATGGAGAAAGTACGGACAAAAAATGATCACAAATGCCAAATATTTCAGGTATATATCTATAGTGCAAATTCAAATAAGAATAAATATTAGTCAACATTATACACTAATGTGATAATTTTAATGTGTTAGATTCCATCAATCTGTAACATTCAAATTAAAAATTAATATAGATAAAGTTAAGTTTAAAATTTAATATGTATGCAGGAGCTACTATAGGTGCACTCACATGAATGATCAACAATGTGAAGCAATCAAACATGTTCAAAGAATACAAAAGAACCCTTCGTTGTACCGGACTACATATTATGGTCATCACACTTGCAAAAGCTCTTACCATTCAGACTTAAATTTGGAATCCATTTTCTCTTCTGATATTGACTCTTCAATATTACTTAGTTTCGATAACATTTCAAGTAAACAAGAATATCCGTTTGGACAGTCACCACCATTGCCACTTCTTGCATCTCCAAAAGGAGATCCCAAGGAAGAGATACATCATGATCATTTTCCTCAAAACCAAATGTTCTCACCGGAGAATCTCCAGTTATGCGATTTTGATGTTTATTTTGATTATTTGAGAGATGTCACTGAGTCATCATCGAGTGAATCTTTTTAA

Protein sequence

>MS.gene63387.t1

MENMLWLPTNYGKKAMEDELMRGRDMANQLLEVLTFDDKSNIREVKGSNTKSSKVLPFNIVAEDLVREVLKSLTNTLLLLNKGKESNDMAVPITPTTIRDFSFSTNCPKMEEDLGGAYKKLKTLNTKNPKVSNKRKSSAPTWEKTASKLIDDGHTWRKYGQKMITNAKYFRSYYRCTHMNDQQCEAIKHVQRIQKNPSLYRTTYYGHHTCKSSYHSDLNLESIFSSDIDSSILLSFDNISSKQEYPFGQSPPLPLLASPKGDPKEEIHHDHFPQNQMFSPENLQLCDFDVYFDYLRDVTESSSSESF