Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63392.t1 | XP_003626547.1 | 94.8 | 174 | 9 | 0 | 1 | 174 | 1 | 174 | 9.20E-91 | 342.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63392.t1 | Q8LGI8 | 90.2 | 174 | 17 | 0 | 1 | 174 | 1 | 174 | 8.8e-90 | 330.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63392.t1 | G7L415 | 94.8 | 174 | 9 | 0 | 1 | 174 | 1 | 174 | 6.7e-91 | 342.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene63392.t1 | TR | GNAT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63392.t1 | MTR_7g117040 | 94.828 | 174 | 9 | 0 | 1 | 174 | 1 | 174 | 2.84e-124 | 347 |
| MS.gene63392.t1 | MTR_7g117040 | 92.529 | 174 | 9 | 1 | 1 | 174 | 1 | 170 | 1.06e-118 | 333 |
| MS.gene63392.t1 | MTR_7g117040 | 90.805 | 174 | 9 | 1 | 1 | 174 | 1 | 167 | 3.13e-116 | 326 |
| MS.gene63392.t1 | MTR_3g034200 | 35.802 | 162 | 99 | 2 | 1 | 161 | 1 | 158 | 1.21e-29 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63392.t1 | AT1G03150 | 90.230 | 174 | 17 | 0 | 1 | 174 | 1 | 174 | 5.85e-120 | 336 |
| MS.gene63392.t1 | AT5G13780 | 37.179 | 156 | 97 | 1 | 1 | 155 | 1 | 156 | 5.23e-29 | 106 |
Find 42 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAGTGCTACGTTATTATTC+TGG | 0.239936 | 2.4:-21617048 | MS.gene63392:CDS |
| GTAACCCATGATTCGATTTC+CGG | 0.242250 | 2.4:+21617787 | None:intergenic |
| TGATTCGATTTCCGGGGCCT+TGG | 0.300853 | 2.4:+21617795 | None:intergenic |
| GTCAGGCCATCTTGCTAAAT+AGG | 0.361124 | 2.4:+21617832 | None:intergenic |
| TTCCGGGGCCTTGGGCGACT+TGG | 0.382516 | 2.4:+21617804 | None:intergenic |
| GATTCGATTTCCGGGGCCTT+GGG | 0.386942 | 2.4:+21617796 | None:intergenic |
| AACTGTTGCCTGCGATAATC+CGG | 0.394043 | 2.4:+21617624 | None:intergenic |
| TAACCCATGATTCGATTTCC+GGG | 0.408074 | 2.4:+21617788 | None:intergenic |
| TAGTCGTATTCTAATTCATC+AGG | 0.411545 | 2.4:+21616845 | None:intergenic |
| TTCTGGGGAAGAAGATGGAT+TGG | 0.420903 | 2.4:-21617031 | MS.gene63392:CDS |
| AGATGGTCAAGATTGACATT+TGG | 0.438959 | 2.4:+21617984 | None:intergenic |
| TATTATTCTGGGGAAGAAGA+TGG | 0.447212 | 2.4:-21617036 | MS.gene63392:CDS |
| TGCTAAATAGGTCATGTAGA+AGG | 0.454880 | 2.4:+21617844 | None:intergenic |
| AGTCCGTCAGCCCTCTTAAA+CGG | 0.466234 | 2.4:-21616877 | MS.gene63392:CDS |
| AACATGAGTACGTACAGTTT+CGG | 0.469338 | 2.4:+21617959 | None:intergenic |
| GAGTGCTACGTTATTATTCT+GGG | 0.470429 | 2.4:-21617047 | MS.gene63392:CDS |
| TCTGGGGAAGAAGATGGATT+GGG | 0.475034 | 2.4:-21617030 | MS.gene63392:intron |
| AACTGCAGTGACCGTTGCTC+CGG | 0.480526 | 2.4:-21617643 | MS.gene63392:CDS |
| TTCCAAGTCGCCCAAGGCCC+CGG | 0.485170 | 2.4:-21617806 | MS.gene63392:CDS |
| GGAGTGACATGCCGTTTAAG+AGG | 0.496131 | 2.4:+21616866 | None:intergenic |
| TAGAGGGGCAAGGAGAATCT+TGG | 0.499019 | 2.4:-21617677 | MS.gene63392:CDS |
| TGGGCGACTTGGAAATAGTC+AGG | 0.503036 | 2.4:+21617815 | None:intergenic |
| TGACATTTGGAAAACGAAGA+AGG | 0.504695 | 2.4:+21617997 | None:intergenic |
| AGCCATCAAGATGTATGAAA+AGG | 0.514376 | 2.4:-21617436 | MS.gene63392:intron |
| AGGCCCCGGAAATCGAATCA+TGG | 0.515528 | 2.4:-21617792 | MS.gene63392:CDS |
| TACGTACAGTTTCGGTGAGA+TGG | 0.530837 | 2.4:+21617967 | None:intergenic |
| ACATGACCTATTTAGCAAGA+TGG | 0.544945 | 2.4:-21617838 | MS.gene63392:CDS |
| GCAGTTATGGGTAAAGTAGA+GGG | 0.544970 | 2.4:-21617693 | MS.gene63392:intron |
| GGCCCCGGAAATCGAATCAT+GGG | 0.555258 | 2.4:-21617791 | MS.gene63392:CDS |
| GACTATTTCCAAGTCGCCCA+AGG | 0.565815 | 2.4:-21617812 | MS.gene63392:CDS |
| ATGGGTAAAGTAGAGGGGCA+AGG | 0.572891 | 2.4:-21617687 | MS.gene63392:CDS |
| TGCAGTTATGGGTAAAGTAG+AGG | 0.579669 | 2.4:-21617694 | MS.gene63392:intron |
| GAGTGACATGCCGTTTAAGA+GGG | 0.585815 | 2.4:+21616867 | None:intergenic |
| CCGTTGCTCCGGATTATCGC+AGG | 0.586411 | 2.4:-21617632 | MS.gene63392:CDS |
| AATAATAACGTAGCACTCGT+CGG | 0.603392 | 2.4:+21617052 | None:intergenic |
| AACCCATGATTCGATTTCCG+GGG | 0.619081 | 2.4:+21617789 | None:intergenic |
| ATGCCGTTTAAGAGGGCTGA+CGG | 0.621153 | 2.4:+21616874 | None:intergenic |
| AAGAGATGACGACGATACGT+AGG | 0.659970 | 2.4:-21618034 | None:intergenic |
| CCTGCGATAATCCGGAGCAA+CGG | 0.660863 | 2.4:+21617632 | None:intergenic |
| GGGCAAGGAGAATCTTGGCA+TGG | 0.661266 | 2.4:-21617672 | MS.gene63392:CDS |
| AGTGCTACGTTATTATTCTG+GGG | 0.665106 | 2.4:-21617046 | MS.gene63392:CDS |
| CAGTTATGGGTAAAGTAGAG+GGG | 0.668038 | 2.4:-21617692 | MS.gene63392:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATGTATAACTTAATCAT+TGG | - | chr2.4:21617672-21617691 | MS.gene63392:CDS | 15.0% |
| !! | AAAAAACCCTATTAGAATTT+GGG | + | chr2.4:21617613-21617632 | None:intergenic | 20.0% |
| !! | AGATTACTTATCAATAGTTT+AGG | - | chr2.4:21617646-21617665 | MS.gene63392:CDS | 20.0% |
| !! | TAAAAAACCCTATTAGAATT+TGG | + | chr2.4:21617614-21617633 | None:intergenic | 20.0% |
| !! | TATAACTTAATCATTGGATA+TGG | - | chr2.4:21617678-21617697 | MS.gene63392:CDS | 20.0% |
| !!! | GATATGTTTTCTTTTAGATT+AGG | - | chr2.4:21617721-21617740 | MS.gene63392:intron | 20.0% |
| ! | AATATCATTCATGCAGTTAT+GGG | - | chr2.4:21617168-21617187 | MS.gene63392:intron | 25.0% |
| ! | ACAGCAAATAAATAGTGATA+CGG | + | chr2.4:21617919-21617938 | None:intergenic | 25.0% |
| ! | TCCTCATATCTGAAAATAAA+GGG | + | chr2.4:21617949-21617968 | None:intergenic | 25.0% |
| ! | TGTGTAAAAGACTATACTAT+GGG | - | chr2.4:21617134-21617153 | MS.gene63392:intron | 25.0% |
| ! | TTCCTCATATCTGAAAATAA+AGG | + | chr2.4:21617950-21617969 | None:intergenic | 25.0% |
| ! | TTGTGTAAAAGACTATACTA+TGG | - | chr2.4:21617133-21617152 | MS.gene63392:intron | 25.0% |
| !!! | TTTATTTTCAGATATGAGGA+AGG | - | chr2.4:21617949-21617968 | MS.gene63392:intron | 25.0% |
| ACATATCCTAATCCAACAAA+TGG | + | chr2.4:21617708-21617727 | None:intergenic | 30.0% | |
| ATCCAACCCAAATTCTAATA+GGG | - | chr2.4:21617604-21617623 | MS.gene63392:CDS | 30.0% | |
| CAAGCTATAAGATGTCAAAA+AGG | + | chr2.4:21617788-21617807 | None:intergenic | 30.0% | |
| CAATATCATTCATGCAGTTA+TGG | - | chr2.4:21617167-21617186 | MS.gene63392:intron | 30.0% | |
| CATAACTGCATGAATGATAT+TGG | + | chr2.4:21617169-21617188 | None:intergenic | 30.0% | |
| TAGTCGTATTCTAATTCATC+AGG | + | chr2.4:21618031-21618050 | None:intergenic | 30.0% | |
| ! | CTTTTTGACATCTTATAGCT+TGG | - | chr2.4:21617786-21617805 | MS.gene63392:CDS | 30.0% |
| ! | TACACAAGGTTGCTTTAAAT+CGG | - | chr2.4:21617518-21617537 | MS.gene63392:intron | 30.0% |
| ! | TACCTTTTCATACATCTTGA+TGG | + | chr2.4:21617442-21617461 | None:intergenic | 30.0% |
| !!! | TCCCTTTATTTTCAGATATG+AGG | - | chr2.4:21617945-21617964 | MS.gene63392:intron | 30.0% |
| AACATGAGTACGTACAGTTT+CGG | + | chr2.4:21616917-21616936 | None:intergenic | 35.0% | |
| AACCCTATTAGAATTTGGGT+TGG | + | chr2.4:21617609-21617628 | None:intergenic | 35.0% | |
| AATAATAACGTAGCACTCGT+CGG | + | chr2.4:21617824-21617843 | None:intergenic | 35.0% | |
| ACATGACCTATTTAGCAAGA+TGG | - | chr2.4:21617035-21617054 | MS.gene63392:CDS | 35.0% | |
| AGATGGTCAAGATTGACATT+TGG | + | chr2.4:21616892-21616911 | None:intergenic | 35.0% | |
| AGCCATCAAGATGTATGAAA+AGG | - | chr2.4:21617437-21617456 | MS.gene63392:CDS | 35.0% | |
| AGTGCTACGTTATTATTCTG+GGG | - | chr2.4:21617827-21617846 | MS.gene63392:CDS | 35.0% | |
| AGTGGAAGGTTTAATCTGAA+AGG | + | chr2.4:21617481-21617500 | None:intergenic | 35.0% | |
| GAGTGCTACGTTATTATTCT+GGG | - | chr2.4:21617826-21617845 | MS.gene63392:CDS | 35.0% | |
| GATCCAACCCAAATTCTAAT+AGG | - | chr2.4:21617603-21617622 | MS.gene63392:CDS | 35.0% | |
| TCCTACAAAATCAGCTTGTA+AGG | + | chr2.4:21617575-21617594 | None:intergenic | 35.0% | |
| TGACATTTGGAAAACGAAGA+AGG | + | chr2.4:21616879-21616898 | None:intergenic | 35.0% | |
| TGCTAAATAGGTCATGTAGA+AGG | + | chr2.4:21617032-21617051 | None:intergenic | 35.0% | |
| ! | TATTATTCTGGGGAAGAAGA+TGG | - | chr2.4:21617837-21617856 | MS.gene63392:CDS | 35.0% |
| !! | ACCTTACAAGCTGATTTTGT+AGG | - | chr2.4:21617571-21617590 | MS.gene63392:intron | 35.0% |
| !! | CACAAAGTGAATTGAGTTGA+GGG | + | chr2.4:21616962-21616981 | None:intergenic | 35.0% |
| !! | TGAATTGAGTTGAGGGTTTA+GGG | + | chr2.4:21616955-21616974 | None:intergenic | 35.0% |
| !! | TTTTCATACATCTTGATGGC+TGG | + | chr2.4:21617438-21617457 | None:intergenic | 35.0% |
| ACCTTGTGTACAAACCTACA+AGG | + | chr2.4:21617508-21617527 | None:intergenic | 40.0% | |
| AGCGACAATATGTAAGGCAT+TGG | - | chr2.4:21617306-21617325 | MS.gene63392:intron | 40.0% | |
| CAGTTATGGGTAAAGTAGAG+GGG | - | chr2.4:21617181-21617200 | MS.gene63392:intron | 40.0% | |
| CGAGTGCTACGTTATTATTC+TGG | - | chr2.4:21617825-21617844 | MS.gene63392:CDS | 40.0% | |
| GACATCAGCGACAATATGTA+AGG | - | chr2.4:21617300-21617319 | MS.gene63392:intron | 40.0% | |
| GCAGTTATGGGTAAAGTAGA+GGG | - | chr2.4:21617180-21617199 | MS.gene63392:intron | 40.0% | |
| GGATATGGACTTCCATTTGT+TGG | - | chr2.4:21617693-21617712 | MS.gene63392:intron | 40.0% | |
| GTAACCCATGATTCGATTTC+CGG | + | chr2.4:21617089-21617108 | None:intergenic | 40.0% | |
| TAACCCATGATTCGATTTCC+GGG | + | chr2.4:21617088-21617107 | None:intergenic | 40.0% | |
| TCCTTGTAGGTTTGTACACA+AGG | - | chr2.4:21617504-21617523 | MS.gene63392:intron | 40.0% | |
| TGCAGTTATGGGTAAAGTAG+AGG | - | chr2.4:21617179-21617198 | MS.gene63392:intron | 40.0% | |
| ! | GCACAAAGTGAATTGAGTTG+AGG | + | chr2.4:21616963-21616982 | None:intergenic | 40.0% |
| ! | GGACTTCCATTTGTTGGATT+AGG | - | chr2.4:21617699-21617718 | MS.gene63392:intron | 40.0% |
| !! | GTGAATTGAGTTGAGGGTTT+AGG | + | chr2.4:21616956-21616975 | None:intergenic | 40.0% |
| AACCCATGATTCGATTTCCG+GGG | + | chr2.4:21617087-21617106 | None:intergenic | 45.0% | |
| AACTGTTGCCTGCGATAATC+CGG | + | chr2.4:21617252-21617271 | None:intergenic | 45.0% | |
| ACAAACCTACAAGGAAGCAG+TGG | + | chr2.4:21617499-21617518 | None:intergenic | 45.0% | |
| GTCAGGCCATCTTGCTAAAT+AGG | + | chr2.4:21617044-21617063 | None:intergenic | 45.0% | |
| GTGTGTTGTGTGTTTGTGTC+TGG | - | chr2.4:21617332-21617351 | MS.gene63392:intron | 45.0% | |
| TACGTACAGTTTCGGTGAGA+TGG | + | chr2.4:21616909-21616928 | None:intergenic | 45.0% | |
| TGTTGTGTGTTTGTGTCTGG+AGG | - | chr2.4:21617335-21617354 | MS.gene63392:intron | 45.0% | |
| ! | GAGTGACATGCCGTTTAAGA+GGG | + | chr2.4:21618009-21618028 | None:intergenic | 45.0% |
| ! | TCTGGGGAAGAAGATGGATT+GGG | - | chr2.4:21617843-21617862 | MS.gene63392:CDS | 45.0% |
| ! | TTCTGGGGAAGAAGATGGAT+TGG | - | chr2.4:21617842-21617861 | MS.gene63392:CDS | 45.0% |
| !! | GGAAGAAGATGGATTGGGTA+TGG | - | chr2.4:21617848-21617867 | MS.gene63392:CDS | 45.0% |
| ACCTACAAGGAAGCAGTGGA+AGG | + | chr2.4:21617495-21617514 | None:intergenic | 50.0% | |
| ACCTTCCACTGCTTCCTTGT+AGG | - | chr2.4:21617491-21617510 | MS.gene63392:intron | 50.0% | |
| AGTCCGTCAGCCCTCTTAAA+CGG | - | chr2.4:21617996-21618015 | MS.gene63392:CDS | 50.0% | |
| ATGGGTAAAGTAGAGGGGCA+AGG | - | chr2.4:21617186-21617205 | MS.gene63392:intron | 50.0% | |
| GACTATTTCCAAGTCGCCCA+AGG | - | chr2.4:21617061-21617080 | MS.gene63392:CDS | 50.0% | |
| TAGAGGGGCAAGGAGAATCT+TGG | - | chr2.4:21617196-21617215 | MS.gene63392:intron | 50.0% | |
| ! | ATGCCGTTTAAGAGGGCTGA+CGG | + | chr2.4:21618002-21618021 | None:intergenic | 50.0% |
| ! | GGAGTGACATGCCGTTTAAG+AGG | + | chr2.4:21618010-21618029 | None:intergenic | 50.0% |
| ! | TGGGCGACTTGGAAATAGTC+AGG | + | chr2.4:21617061-21617080 | None:intergenic | 50.0% |
| AACTGCAGTGACCGTTGCTC+CGG | - | chr2.4:21617230-21617249 | MS.gene63392:intron | 55.0% | |
| AGGCCCCGGAAATCGAATCA+TGG | - | chr2.4:21617081-21617100 | MS.gene63392:intron | 55.0% | |
| CCTGCGATAATCCGGAGCAA+CGG | + | chr2.4:21617244-21617263 | None:intergenic | 55.0% | |
| GATTCGATTTCCGGGGCCTT+GGG | + | chr2.4:21617080-21617099 | None:intergenic | 55.0% | |
| GGCCCCGGAAATCGAATCAT+GGG | - | chr2.4:21617082-21617101 | MS.gene63392:intron | 55.0% | |
| GGGCAAGGAGAATCTTGGCA+TGG | - | chr2.4:21617201-21617220 | MS.gene63392:intron | 55.0% | |
| TGATTCGATTTCCGGGGCCT+TGG | + | chr2.4:21617081-21617100 | None:intergenic | 55.0% | |
| CCGTTGCTCCGGATTATCGC+AGG | - | chr2.4:21617241-21617260 | MS.gene63392:intron | 60.0% | |
| TTCCAAGTCGCCCAAGGCCC+CGG | - | chr2.4:21617067-21617086 | MS.gene63392:CDS | 65.0% | |
| TTCCGGGGCCTTGGGCGACT+TGG | + | chr2.4:21617072-21617091 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 21616844 | 21618051 | 21616844 | ID=MS.gene63392 |
| chr2.4 | mRNA | 21616844 | 21618051 | 21616844 | ID=MS.gene63392.t1;Parent=MS.gene63392 |
| chr2.4 | exon | 21617974 | 21618051 | 21617974 | ID=MS.gene63392.t1.exon1;Parent=MS.gene63392.t1 |
| chr2.4 | CDS | 21617974 | 21618051 | 21617974 | ID=cds.MS.gene63392.t1;Parent=MS.gene63392.t1 |
| chr2.4 | exon | 21617786 | 21617876 | 21617786 | ID=MS.gene63392.t1.exon2;Parent=MS.gene63392.t1 |
| chr2.4 | CDS | 21617786 | 21617876 | 21617786 | ID=cds.MS.gene63392.t1;Parent=MS.gene63392.t1 |
| chr2.4 | exon | 21617579 | 21617711 | 21617579 | ID=MS.gene63392.t1.exon3;Parent=MS.gene63392.t1 |
| chr2.4 | CDS | 21617579 | 21617711 | 21617579 | ID=cds.MS.gene63392.t1;Parent=MS.gene63392.t1 |
| chr2.4 | exon | 21617437 | 21617506 | 21617437 | ID=MS.gene63392.t1.exon4;Parent=MS.gene63392.t1 |
| chr2.4 | CDS | 21617437 | 21617506 | 21617437 | ID=cds.MS.gene63392.t1;Parent=MS.gene63392.t1 |
| chr2.4 | exon | 21617031 | 21617091 | 21617031 | ID=MS.gene63392.t1.exon5;Parent=MS.gene63392.t1 |
| chr2.4 | CDS | 21617031 | 21617091 | 21617031 | ID=cds.MS.gene63392.t1;Parent=MS.gene63392.t1 |
| chr2.4 | exon | 21616844 | 21616935 | 21616844 | ID=MS.gene63392.t1.exon6;Parent=MS.gene63392.t1 |
| chr2.4 | CDS | 21616844 | 21616935 | 21616844 | ID=cds.MS.gene63392.t1;Parent=MS.gene63392.t1 |
| Gene Sequence |
| Protein sequence |
