Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63485.t1 | XP_013460707.1 | 93.8 | 260 | 15 | 1 | 1 | 259 | 1 | 260 | 3.60E-139 | 504.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63485.t1 | O80942 | 50.4 | 113 | 43 | 3 | 33 | 135 | 18 | 127 | 2.5e-16 | 87.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63485.t1 | A0A072UZ72 | 93.8 | 260 | 15 | 1 | 1 | 259 | 1 | 260 | 2.6e-139 | 504.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene63485.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63485.t1 | MTR_3g068095 | 93.846 | 260 | 15 | 1 | 1 | 259 | 1 | 260 | 0.0 | 515 |
| MS.gene63485.t1 | MTR_5g080660 | 47.287 | 258 | 94 | 13 | 8 | 259 | 11 | 232 | 1.93e-51 | 168 |
| MS.gene63485.t1 | MTR_4g021270 | 56.311 | 103 | 31 | 3 | 33 | 121 | 36 | 138 | 1.17e-29 | 114 |
| MS.gene63485.t1 | MTR_5g014400 | 71.930 | 57 | 15 | 1 | 33 | 89 | 59 | 114 | 4.33e-23 | 94.7 |
| MS.gene63485.t1 | MTR_2g076060 | 49.398 | 83 | 39 | 1 | 10 | 89 | 2 | 84 | 2.94e-19 | 84.0 |
| MS.gene63485.t1 | MTR_1g070265 | 80.952 | 42 | 8 | 0 | 49 | 90 | 29 | 70 | 1.07e-17 | 79.0 |
| MS.gene63485.t1 | MTR_7g063220 | 70.000 | 50 | 15 | 0 | 55 | 104 | 24 | 73 | 5.49e-16 | 73.2 |
| MS.gene63485.t1 | MTR_4g064873 | 62.500 | 48 | 18 | 0 | 54 | 101 | 20 | 67 | 1.27e-14 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63485.t1 | AT2G37740 | 75.439 | 57 | 11 | 1 | 33 | 89 | 18 | 71 | 5.61e-24 | 98.6 |
| MS.gene63485.t1 | AT4G17810 | 30.804 | 224 | 113 | 4 | 30 | 253 | 19 | 200 | 1.30e-23 | 95.1 |
| MS.gene63485.t1 | AT5G06070 | 37.603 | 242 | 106 | 10 | 25 | 259 | 22 | 225 | 6.72e-23 | 94.0 |
| MS.gene63485.t1 | AT4G17810 | 69.091 | 55 | 17 | 0 | 30 | 84 | 19 | 73 | 1.12e-22 | 92.0 |
| MS.gene63485.t1 | AT3G23130 | 82.927 | 41 | 7 | 0 | 47 | 87 | 37 | 77 | 5.27e-18 | 80.1 |
| MS.gene63485.t1 | AT2G42410 | 78.049 | 41 | 9 | 0 | 48 | 88 | 40 | 80 | 2.81e-17 | 78.6 |
| MS.gene63485.t1 | AT2G42410 | 78.049 | 41 | 9 | 0 | 48 | 88 | 40 | 80 | 2.81e-17 | 78.6 |
| MS.gene63485.t1 | AT2G42410 | 78.049 | 41 | 9 | 0 | 48 | 88 | 66 | 106 | 3.25e-17 | 79.0 |
| MS.gene63485.t1 | AT3G09290 | 63.636 | 44 | 16 | 0 | 55 | 98 | 33 | 76 | 1.08e-12 | 65.1 |
| MS.gene63485.t1 | AT3G53820 | 68.571 | 35 | 11 | 0 | 55 | 89 | 19 | 53 | 3.57e-11 | 60.1 |
Find 47 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATATTCTTCTTCAATTAGA+TGG | 0.176609 | 3.1:-56388049 | MS.gene63485:CDS |
| TGTTGAAACAAGTTTGTCTA+TGG | 0.217128 | 3.1:-56387937 | MS.gene63485:CDS |
| TTCCCAAGACTTGTTGTTGA+TGG | 0.233876 | 3.1:+56388436 | None:intergenic |
| TTTAGGTCTGCTCAAGCTTT+AGG | 0.265540 | 3.1:-56388323 | MS.gene63485:CDS |
| AATGAAGTTCTAATTCATTA+TGG | 0.326510 | 3.1:+56388243 | None:intergenic |
| GGACAAAATAATTCAATGTT+TGG | 0.329063 | 3.1:-56387915 | MS.gene63485:CDS |
| AGATCCAAGTCTTCTATTCC+AGG | 0.334055 | 3.1:+56387726 | None:intergenic |
| GAAGAAATGGAAGCTGTCTT+TGG | 0.357856 | 3.1:+56387831 | None:intergenic |
| GGATACTTGGTGGATGTATA+TGG | 0.358230 | 3.1:-56388385 | MS.gene63485:CDS |
| CAGTCTGCAGAGTTTGTGGT+TGG | 0.405826 | 3.1:-56387756 | MS.gene63485:CDS |
| TAAATATGATGTGGATGAAA+AGG | 0.412314 | 3.1:-56388505 | MS.gene63485:CDS |
| GACAGGCAGTGAAGAAGAAA+TGG | 0.412947 | 3.1:+56387818 | None:intergenic |
| TCTTGTGTAGATGCAGTTGA+TGG | 0.423703 | 3.1:+56388098 | None:intergenic |
| AATGAAGGAGTGCCATCATT+TGG | 0.429411 | 3.1:-56388461 | MS.gene63485:CDS |
| GAAGAATATGAAGACATGTT+TGG | 0.429891 | 3.1:+56388062 | None:intergenic |
| ATATTCTTCTTCAATTAGAT+GGG | 0.438910 | 3.1:-56388048 | MS.gene63485:CDS |
| GAAGATGCAGCTAGGATACT+TGG | 0.443307 | 3.1:-56388398 | MS.gene63485:CDS |
| GAACCATCAACAACAAGTCT+TGG | 0.447185 | 3.1:-56388439 | MS.gene63485:CDS |
| ACTTCTGTAAAAGAGAGTTT+AGG | 0.451370 | 3.1:-56388340 | MS.gene63485:CDS |
| TTGGATCTTGAACTCAGGCT+TGG | 0.454061 | 3.1:-56387711 | MS.gene63485:CDS |
| TGGATCTTGAACTCAGGCTT+GGG | 0.459255 | 3.1:-56387710 | MS.gene63485:CDS |
| AAACATGGCTTCAGCAAGAC+AGG | 0.470524 | 3.1:+56387801 | None:intergenic |
| TTTGTGGTTGGAGTTAAGCC+TGG | 0.483977 | 3.1:-56387744 | MS.gene63485:CDS |
| TTGTTGATGGTTCCAAATGA+TGG | 0.490417 | 3.1:+56388449 | None:intergenic |
| AACCATCAACAACAAGTCTT+GGG | 0.510991 | 3.1:-56388438 | MS.gene63485:CDS |
| GTTGAAACAAGTTTGTCTAT+GGG | 0.512192 | 3.1:-56387936 | MS.gene63485:CDS |
| AAGTGACATGAGTAAGATCT+TGG | 0.519917 | 3.1:+56388359 | None:intergenic |
| AAGACTTGGATCTTGAACTC+AGG | 0.538252 | 3.1:-56387716 | MS.gene63485:CDS |
| GAGTCTCTTGATTCTGAACA+AGG | 0.543085 | 3.1:-56388011 | MS.gene63485:CDS |
| AGGTCTGCTCAAGCTTTAGG+TGG | 0.544624 | 3.1:-56388320 | MS.gene63485:CDS |
| AACAAGGGCTAGCTAATGCA+AGG | 0.555187 | 3.1:-56387995 | MS.gene63485:CDS |
| TAAGCCTGGAATAGAAGACT+TGG | 0.563435 | 3.1:-56387730 | MS.gene63485:CDS |
| ATCTTGCACTCACACAATGA+AGG | 0.564898 | 3.1:-56388476 | MS.gene63485:CDS |
| GCAGAGATAACTCTTCTACT+TGG | 0.579663 | 3.1:-56388121 | MS.gene63485:CDS |
| TATTCTTCTTCAATTAGATG+GGG | 0.582180 | 3.1:-56388047 | MS.gene63485:CDS |
| GATGCAGCTAGGATACTTGG+TGG | 0.596955 | 3.1:-56388395 | MS.gene63485:CDS |
| TGACATGAGTAAGATCTTGG+TGG | 0.606437 | 3.1:+56388362 | None:intergenic |
| ACTGATGATGATTCATGTGG+TGG | 0.616045 | 3.1:-56387879 | MS.gene63485:CDS |
| CTGATGATGATTCATGTGGT+GGG | 0.621213 | 3.1:-56387878 | MS.gene63485:CDS |
| ATTCTTCTTCAATTAGATGG+GGG | 0.625385 | 3.1:-56388046 | MS.gene63485:CDS |
| AGGAATCACTAAATATGATG+TGG | 0.652487 | 3.1:-56388514 | MS.gene63485:CDS |
| AAACATCTATCATTCAAACA+TGG | 0.662081 | 3.1:+56387786 | None:intergenic |
| TGATGATGATTCATGTGGTG+GGG | 0.671193 | 3.1:-56387877 | MS.gene63485:CDS |
| AGTCTCTTGATTCTGAACAA+GGG | 0.707551 | 3.1:-56388010 | MS.gene63485:CDS |
| ACAAGGGCTAGCTAATGCAA+GGG | 0.711358 | 3.1:-56387994 | MS.gene63485:CDS |
| CAAACTGATGATGATTCATG+TGG | 0.716039 | 3.1:-56387882 | MS.gene63485:CDS |
| CAGAATCAAGAGACTCCATG+TGG | 0.748859 | 3.1:+56388018 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATTCTTCTTCAATTAGAT+GGG | - | chr3.1:56388159-56388178 | MS.gene63485:intron | 20.0% |
| !! | CTAATTATTGTTCTAACTTA+TGG | - | chr3.1:56388049-56388068 | MS.gene63485:CDS | 20.0% |
| !!! | AATGAAGTTCTAATTCATTA+TGG | + | chr3.1:56387967-56387986 | None:intergenic | 20.0% |
| ! | AAACATCTATCATTCAAACA+TGG | + | chr3.1:56388424-56388443 | None:intergenic | 25.0% |
| ! | CATATTCTTCTTCAATTAGA+TGG | - | chr3.1:56388158-56388177 | MS.gene63485:intron | 25.0% |
| ! | GGACAAAATAATTCAATGTT+TGG | - | chr3.1:56388292-56388311 | MS.gene63485:CDS | 25.0% |
| ! | TAAATATGATGTGGATGAAA+AGG | - | chr3.1:56387702-56387721 | MS.gene63485:CDS | 25.0% |
| ! | TATTCTTCTTCAATTAGATG+GGG | - | chr3.1:56388160-56388179 | MS.gene63485:intron | 25.0% |
| !! | GAACAAATTTTCAAAGGTTA+TGG | - | chr3.1:56388235-56388254 | MS.gene63485:CDS | 25.0% |
| ACTTCTGTAAAAGAGAGTTT+AGG | - | chr3.1:56387867-56387886 | MS.gene63485:CDS | 30.0% | |
| AGGAATCACTAAATATGATG+TGG | - | chr3.1:56387693-56387712 | MS.gene63485:CDS | 30.0% | |
| ATTCTTCTTCAATTAGATGG+GGG | - | chr3.1:56388161-56388180 | MS.gene63485:intron | 30.0% | |
| GAAGAATATGAAGACATGTT+TGG | + | chr3.1:56388148-56388167 | None:intergenic | 30.0% | |
| GTTGAAACAAGTTTGTCTAT+GGG | - | chr3.1:56388271-56388290 | MS.gene63485:CDS | 30.0% | |
| TGTTGAAACAAGTTTGTCTA+TGG | - | chr3.1:56388270-56388289 | MS.gene63485:CDS | 30.0% | |
| ! | CATCATCAGTTTGCAATTTT+TGG | + | chr3.1:56388318-56388337 | None:intergenic | 30.0% |
| AACCATCAACAACAAGTCTT+GGG | - | chr3.1:56387769-56387788 | MS.gene63485:CDS | 35.0% | |
| AAGTGACATGAGTAAGATCT+TGG | + | chr3.1:56387851-56387870 | None:intergenic | 35.0% | |
| AGTCTCTTGATTCTGAACAA+GGG | - | chr3.1:56388197-56388216 | MS.gene63485:intron | 35.0% | |
| ! | CAAACTGATGATGATTCATG+TGG | - | chr3.1:56388325-56388344 | MS.gene63485:CDS | 35.0% |
| ! | GCAAGGGAACAAATTTTCAA+AGG | - | chr3.1:56388229-56388248 | MS.gene63485:CDS | 35.0% |
| !! | TTGTTGATGGTTCCAAATGA+TGG | + | chr3.1:56387761-56387780 | None:intergenic | 35.0% |
| AAGACTTGGATCTTGAACTC+AGG | - | chr3.1:56388491-56388510 | MS.gene63485:CDS | 40.0% | |
| AGATCCAAGTCTTCTATTCC+AGG | + | chr3.1:56388484-56388503 | None:intergenic | 40.0% | |
| ATCTTGCACTCACACAATGA+AGG | - | chr3.1:56387731-56387750 | MS.gene63485:CDS | 40.0% | |
| GAACCATCAACAACAAGTCT+TGG | - | chr3.1:56387768-56387787 | MS.gene63485:CDS | 40.0% | |
| GAAGAAATGGAAGCTGTCTT+TGG | + | chr3.1:56388379-56388398 | None:intergenic | 40.0% | |
| GAGTCTCTTGATTCTGAACA+AGG | - | chr3.1:56388196-56388215 | MS.gene63485:intron | 40.0% | |
| GCAGAGATAACTCTTCTACT+TGG | - | chr3.1:56388086-56388105 | MS.gene63485:CDS | 40.0% | |
| TAAGCCTGGAATAGAAGACT+TGG | - | chr3.1:56388477-56388496 | MS.gene63485:CDS | 40.0% | |
| TCTTGTGTAGATGCAGTTGA+TGG | + | chr3.1:56388112-56388131 | None:intergenic | 40.0% | |
| TGACATGAGTAAGATCTTGG+TGG | + | chr3.1:56387848-56387867 | None:intergenic | 40.0% | |
| TTTAGGTCTGCTCAAGCTTT+AGG | - | chr3.1:56387884-56387903 | MS.gene63485:CDS | 40.0% | |
| ! | ACTGATGATGATTCATGTGG+TGG | - | chr3.1:56388328-56388347 | MS.gene63485:CDS | 40.0% |
| ! | CTGATGATGATTCATGTGGT+GGG | - | chr3.1:56388329-56388348 | MS.gene63485:CDS | 40.0% |
| ! | GGATACTTGGTGGATGTATA+TGG | - | chr3.1:56387822-56387841 | MS.gene63485:CDS | 40.0% |
| ! | TGATGATGATTCATGTGGTG+GGG | - | chr3.1:56388330-56388349 | MS.gene63485:CDS | 40.0% |
| ! | TTTTCAGTCTGCAGAGTTTG+TGG | - | chr3.1:56388447-56388466 | MS.gene63485:CDS | 40.0% |
| !! | AATGAAGGAGTGCCATCATT+TGG | - | chr3.1:56387746-56387765 | MS.gene63485:CDS | 40.0% |
| !! | TTCCCAAGACTTGTTGTTGA+TGG | + | chr3.1:56387774-56387793 | None:intergenic | 40.0% |
| AAACATGGCTTCAGCAAGAC+AGG | + | chr3.1:56388409-56388428 | None:intergenic | 45.0% | |
| CAGAATCAAGAGACTCCATG+TGG | + | chr3.1:56388192-56388211 | None:intergenic | 45.0% | |
| GAAGATGCAGCTAGGATACT+TGG | - | chr3.1:56387809-56387828 | MS.gene63485:CDS | 45.0% | |
| GACAGGCAGTGAAGAAGAAA+TGG | + | chr3.1:56388392-56388411 | None:intergenic | 45.0% | |
| TAGATGGGGGAATAACCACA+TGG | - | chr3.1:56388174-56388193 | MS.gene63485:intron | 45.0% | |
| TGGATCTTGAACTCAGGCTT+GGG | - | chr3.1:56388497-56388516 | MS.gene63485:CDS | 45.0% | |
| TTGGATCTTGAACTCAGGCT+TGG | - | chr3.1:56388496-56388515 | MS.gene63485:CDS | 45.0% | |
| TTTGTGGTTGGAGTTAAGCC+TGG | - | chr3.1:56388463-56388482 | MS.gene63485:CDS | 45.0% | |
| ! | CTTTTGCAGAAGATGCAGCT+AGG | - | chr3.1:56387801-56387820 | MS.gene63485:CDS | 45.0% |
| !! | AACAAGGGCTAGCTAATGCA+AGG | - | chr3.1:56388212-56388231 | MS.gene63485:intron | 45.0% |
| !! | ACAAGGGCTAGCTAATGCAA+GGG | - | chr3.1:56388213-56388232 | MS.gene63485:intron | 45.0% |
| CAGTCTGCAGAGTTTGTGGT+TGG | - | chr3.1:56388451-56388470 | MS.gene63485:CDS | 50.0% | |
| GATGCAGCTAGGATACTTGG+TGG | - | chr3.1:56387812-56387831 | MS.gene63485:CDS | 50.0% | |
| !! | AGGTCTGCTCAAGCTTTAGG+TGG | - | chr3.1:56387887-56387906 | MS.gene63485:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 56387689 | 56388540 | 56387689 | ID=MS.gene63485 |
| chr3.1 | mRNA | 56387689 | 56388540 | 56387689 | ID=MS.gene63485.t1;Parent=MS.gene63485 |
| chr3.1 | exon | 56388225 | 56388540 | 56388225 | ID=MS.gene63485.t1.exon1;Parent=MS.gene63485.t1 |
| chr3.1 | CDS | 56388225 | 56388540 | 56388225 | ID=cds.MS.gene63485.t1;Parent=MS.gene63485.t1 |
| chr3.1 | exon | 56387689 | 56388152 | 56387689 | ID=MS.gene63485.t1.exon2;Parent=MS.gene63485.t1 |
| chr3.1 | CDS | 56387689 | 56388152 | 56387689 | ID=cds.MS.gene63485.t1;Parent=MS.gene63485.t1 |
| Gene Sequence |
| Protein sequence |
