Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63627.t1 | RHN66404.1 | 86.1 | 187 | 26 | 0 | 1 | 187 | 1 | 187 | 3.60E-72 | 281.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63627.t1 | Q8GTS1 | 46.1 | 167 | 81 | 4 | 4 | 162 | 10 | 175 | 1.3e-25 | 117.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63627.t1 | A0A396IPX9 | 86.1 | 187 | 26 | 0 | 1 | 187 | 1 | 187 | 2.6e-72 | 281.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene63627.t1 | TF | bZIP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63627.t1 | MTR_4g073100 | 45.320 | 203 | 85 | 5 | 1 | 183 | 1 | 197 | 2.16e-51 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63627.t1 | AT3G51960 | 50.000 | 168 | 74 | 4 | 4 | 162 | 10 | 176 | 3.77e-48 | 157 |
| MS.gene63627.t1 | AT3G51960 | 50.299 | 167 | 74 | 4 | 4 | 162 | 10 | 175 | 4.60e-48 | 156 |
| MS.gene63627.t1 | AT3G51960 | 56.250 | 128 | 50 | 1 | 41 | 162 | 10 | 137 | 6.98e-48 | 154 |
| MS.gene63627.t1 | AT2G16770 | 52.288 | 153 | 60 | 5 | 29 | 169 | 18 | 169 | 5.22e-47 | 154 |
| MS.gene63627.t1 | AT2G16770 | 52.288 | 153 | 60 | 5 | 29 | 169 | 18 | 169 | 5.22e-47 | 154 |
| MS.gene63627.t1 | AT4G35040 | 47.541 | 183 | 72 | 8 | 1 | 165 | 1 | 177 | 8.87e-45 | 149 |
| MS.gene63627.t1 | AT4G35040 | 47.541 | 183 | 72 | 8 | 1 | 165 | 1 | 177 | 8.87e-45 | 149 |
| MS.gene63627.t1 | AT4G35040 | 47.541 | 183 | 72 | 8 | 1 | 165 | 1 | 177 | 8.87e-45 | 149 |
| MS.gene63627.t1 | AT4G35040 | 47.541 | 183 | 72 | 8 | 1 | 165 | 1 | 177 | 8.87e-45 | 149 |
Find 22 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGGCAGAAACAGCTTATT+TGG | 0.185081 | 3.2:+23718153 | MS.gene63627:CDS |
| TGAAAATTGCTACATGATTC+TGG | 0.201465 | 3.2:-23717908 | None:intergenic |
| GGAAAAGTTGACAATGAATT+AGG | 0.247864 | 3.2:+23718292 | MS.gene63627:CDS |
| GGCAAGGTTAAGGAACATTT+TGG | 0.264303 | 3.2:+23718258 | MS.gene63627:CDS |
| AAAGTATAGGGAGAAGAAAA+AGG | 0.319200 | 3.2:+23718135 | MS.gene63627:CDS |
| GGCACTGCTTGAAGCTGAAT+TGG | 0.353982 | 3.2:+23718237 | MS.gene63627:CDS |
| AAGCTGAATTGGCAAGGTTA+AGG | 0.385542 | 3.2:+23718248 | MS.gene63627:CDS |
| ACATGCAGGATCATCATCTA+AGG | 0.417339 | 3.2:+23718354 | MS.gene63627:CDS |
| ACACAAATTCAAGGCCAAAA+AGG | 0.422328 | 3.2:+23718086 | MS.gene63627:CDS |
| GTCCATTAGTATTACTCATG+TGG | 0.478158 | 3.2:-23718400 | None:intergenic |
| AAGAAAGAAAATATGGAAGA+TGG | 0.479572 | 3.2:+23717836 | None:intergenic |
| CTGAAGACGACACAAATTCA+AGG | 0.523320 | 3.2:+23718077 | MS.gene63627:CDS |
| CCATGTTTGCATCCACATGC+AGG | 0.525489 | 3.2:+23718340 | MS.gene63627:CDS |
| TTTAAGGAAACTACAAGGGC+AGG | 0.534031 | 3.2:+23718216 | MS.gene63627:CDS |
| CCTGCATGTGGATGCAAACA+TGG | 0.553344 | 3.2:-23718340 | None:intergenic |
| TGTGTGTGATAAGCATCATC+AGG | 0.565504 | 3.2:-23718019 | None:intergenic |
| TGCTTGAAGCTGAATTGGCA+AGG | 0.581638 | 3.2:+23718242 | MS.gene63627:CDS |
| TGATAAGCATCATCAGGACC+AGG | 0.614933 | 3.2:-23718013 | None:intergenic |
| ACTGTGTTCATAGATGTTGA+AGG | 0.626655 | 3.2:-23717932 | None:intergenic |
| TTAGATGATGATCCTGCATG+TGG | 0.661059 | 3.2:-23718352 | None:intergenic |
| ACTCACACTTGTAATCCACC+TGG | 0.661329 | 3.2:+23717995 | MS.gene63627:CDS |
| TAAGCATCATCAGGACCAGG+TGG | 0.696090 | 3.2:-23718010 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAGCTGTTAAAAAGTATA+GGG | + | chr3.2:23718123-23718142 | MS.gene63627:CDS | 25.0% |
| !! | ATGTGAATCAACAACTTTTA+AGG | + | chr3.2:23718200-23718219 | MS.gene63627:CDS | 25.0% |
| !!! | AACTTTTAAGGAAACTACAA+GGG | + | chr3.2:23718212-23718231 | MS.gene63627:CDS | 25.0% |
| AAAGTATAGGGAGAAGAAAA+AGG | + | chr3.2:23718135-23718154 | MS.gene63627:CDS | 30.0% | |
| GGAAAAGTTGACAATGAATT+AGG | + | chr3.2:23718292-23718311 | MS.gene63627:CDS | 30.0% | |
| TGAAAATTGCTACATGATTC+TGG | - | chr3.2:23717911-23717930 | None:intergenic | 30.0% | |
| ! | ATGGCAGATTTTTCTCAAAA+GGG | - | chr3.2:23718324-23718343 | None:intergenic | 30.0% |
| ! | GAGAAGCTGTTAAAAAGTAT+AGG | + | chr3.2:23718122-23718141 | MS.gene63627:CDS | 30.0% |
| !! | CAACTTTTAAGGAAACTACA+AGG | + | chr3.2:23718211-23718230 | MS.gene63627:CDS | 30.0% |
| AAAGGCAGAAACAGCTTATT+TGG | + | chr3.2:23718153-23718172 | MS.gene63627:CDS | 35.0% | |
| ACACAAATTCAAGGCCAAAA+AGG | + | chr3.2:23718086-23718105 | MS.gene63627:CDS | 35.0% | |
| ACTGTGTTCATAGATGTTGA+AGG | - | chr3.2:23717935-23717954 | None:intergenic | 35.0% | |
| ! | CATGGCAGATTTTTCTCAAA+AGG | - | chr3.2:23718325-23718344 | None:intergenic | 35.0% |
| ! | CTATTTCCAGAAGTCCTTTT+TGG | - | chr3.2:23718103-23718122 | None:intergenic | 35.0% |
| !! | AACATTTTGGTGCAGCTTAA+GGG | + | chr3.2:23718271-23718290 | MS.gene63627:CDS | 35.0% |
| !! | AGTGTGAATGCATGTTTTTG+TGG | - | chr3.2:23717978-23717997 | None:intergenic | 35.0% |
| AAGCTGAATTGGCAAGGTTA+AGG | + | chr3.2:23718248-23718267 | MS.gene63627:CDS | 40.0% | |
| ACATGCAGGATCATCATCTA+AGG | + | chr3.2:23718354-23718373 | MS.gene63627:CDS | 40.0% | |
| CTGAAGACGACACAAATTCA+AGG | + | chr3.2:23718077-23718096 | MS.gene63627:CDS | 40.0% | |
| TGTGTGTGATAAGCATCATC+AGG | - | chr3.2:23718022-23718041 | None:intergenic | 40.0% | |
| TTAGATGATGATCCTGCATG+TGG | - | chr3.2:23718355-23718374 | None:intergenic | 40.0% | |
| TTTAAGGAAACTACAAGGGC+AGG | + | chr3.2:23718216-23718235 | MS.gene63627:CDS | 40.0% | |
| ! | GGCAAGGTTAAGGAACATTT+TGG | + | chr3.2:23718258-23718277 | MS.gene63627:CDS | 40.0% |
| !! | GAACATTTTGGTGCAGCTTA+AGG | + | chr3.2:23718270-23718289 | MS.gene63627:CDS | 40.0% |
| ACTCACACTTGTAATCCACC+TGG | + | chr3.2:23717995-23718014 | MS.gene63627:CDS | 45.0% | |
| TCAAGGCCAAAAAGGACTTC+TGG | + | chr3.2:23718094-23718113 | MS.gene63627:CDS | 45.0% | |
| TGATAAGCATCATCAGGACC+AGG | - | chr3.2:23718016-23718035 | None:intergenic | 45.0% | |
| TGCTTGAAGCTGAATTGGCA+AGG | + | chr3.2:23718242-23718261 | MS.gene63627:CDS | 45.0% | |
| CCATGTTTGCATCCACATGC+AGG | + | chr3.2:23718340-23718359 | MS.gene63627:CDS | 50.0% | |
| CCTGCATGTGGATGCAAACA+TGG | - | chr3.2:23718343-23718362 | None:intergenic | 50.0% | |
| GGCACTGCTTGAAGCTGAAT+TGG | + | chr3.2:23718237-23718256 | MS.gene63627:CDS | 50.0% | |
| TAAGCATCATCAGGACCAGG+TGG | - | chr3.2:23718013-23718032 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 23717848 | 23718411 | 23717848 | ID=MS.gene63627 |
| chr3.2 | mRNA | 23717848 | 23718411 | 23717848 | ID=MS.gene63627.t1;Parent=MS.gene63627 |
| chr3.2 | exon | 23717848 | 23718411 | 23717848 | ID=MS.gene63627.t1.exon1;Parent=MS.gene63627.t1 |
| chr3.2 | CDS | 23717848 | 23718411 | 23717848 | ID=cds.MS.gene63627.t1;Parent=MS.gene63627.t1 |
| Gene Sequence |
| Protein sequence |
