AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64028

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64028.t1 MTR_1g025500 97.647 1020 24 0 1 1020 1 1020 0.0 2041
MS.gene64028.t1 MTR_6g007787 29.842 506 228 29 599 1020 1127 1589 1.90e-16 85.5
MS.gene64028.t1 MTR_6g007787 34.667 150 90 5 1 148 254 397 5.75e-14 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64028.t1 AT4G24200 39.487 585 283 25 472 1020 451 1000 6.80e-71 256
MS.gene64028.t1 AT4G24200 39.487 585 283 25 472 1020 451 1000 6.80e-71 256
MS.gene64028.t1 AT3G48060 27.976 504 237 25 599 1007 1136 1608 1.35e-15 82.8
MS.gene64028.t1 AT3G48060 27.976 504 237 25 599 1007 1135 1607 1.50e-15 82.4
MS.gene64028.t1 AT3G48060 27.976 504 237 25 599 1007 1135 1607 1.50e-15 82.4
MS.gene64028.t1 AT3G48050 27.843 510 232 27 599 1007 1133 1607 1.61e-15 82.4
MS.gene64028.t1 AT3G48050 27.843 510 232 27 599 1007 1133 1607 1.61e-15 82.4

Find 251 sgRNAs with CRISPR-Local

Find 291 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGGAGCTGGTGGCGTTTC+AGG 0.062744 1.3:+802335 MS.gene64028:CDS
TGCTCCTGCAATATCGATTT+TGG 0.128104 1.3:+802214 MS.gene64028:CDS
GAATCTTCAGGCTTGCCTTC+TGG 0.146600 1.3:+801900 MS.gene64028:CDS
AAGAGCATGTTCCTCAAATT+TGG 0.207541 1.3:+802261 MS.gene64028:CDS
GTACTTTCAAACTGATTTAC+TGG 0.207850 1.3:+802133 MS.gene64028:CDS
GGTCACAATGTTAGCGCTTC+TGG 0.217592 1.3:-801193 None:intergenic
GATTCAGGCTTAGGATTTAT+TGG 0.225150 1.3:-800703 None:intergenic
TGTGATAAAGTAATAGTTTC+TGG 0.230722 1.3:+800829 MS.gene64028:CDS
TTTGATCTTAATGAAGAATA+TGG 0.251619 1.3:+801573 MS.gene64028:CDS
AATAGTGCCTGAATCTTCTT+TGG 0.260722 1.3:+800774 MS.gene64028:CDS
AATCTTCAGGCTTGCCTTCT+GGG 0.262552 1.3:+801901 MS.gene64028:CDS
CCAGCCATGCTCATAATATA+TGG 0.269350 1.3:-802557 None:intergenic
AAGAAAATCTTGCCTTTGTT+TGG 0.283515 1.3:-801823 None:intergenic
ATGGAAGGGCAGCTCTTCTT+TGG 0.286429 1.3:+802023 MS.gene64028:CDS
AAGTTCAATTTCAGAATTCT+TGG 0.310934 1.3:-801214 None:intergenic
TTAACATTAGCAGCCTTATC+TGG 0.317575 1.3:-800406 None:intergenic
TTCAATACTTGAAGAAGATT+TGG 0.318707 1.3:-802168 None:intergenic
TCTGAAATTGAACTTGACTA+TGG 0.321868 1.3:+801222 MS.gene64028:CDS
TCTTTGGAAGAAATGGCTAT+TGG 0.327878 1.3:+802039 MS.gene64028:CDS
TCTTCAAGTATTGAAGTATA+TGG 0.328883 1.3:+802176 MS.gene64028:CDS
TCTGACATGGAACTTGATTA+TGG 0.330891 1.3:+801057 MS.gene64028:CDS
TCAAAACCTGCACAGACTTC+AGG 0.334253 1.3:+801660 MS.gene64028:CDS
TCCAAGTCAGGGATGCATTC+TGG 0.341426 1.3:-801486 None:intergenic
CTTCAACAATCCTCAAGTTC+AGG 0.342590 1.3:+802728 MS.gene64028:CDS
GTCCTTCTAATTGAATAAAA+AGG 0.346830 1.3:-799970 None:intergenic
GGCTCAACAGCCTTGCCATT+TGG 0.349494 1.3:-802290 None:intergenic
AACTCGGGTTTCATGATTGA+TGG 0.353921 1.3:+802632 MS.gene64028:CDS
GAAAGTGAATTGACATCGTC+TGG 0.354075 1.3:+800550 MS.gene64028:CDS
AAACAAATTGGTGAATCTTC+AGG 0.355173 1.3:+801888 MS.gene64028:CDS
AAAGAAGATTCAGGCACTAT+TGG 0.361886 1.3:-800772 None:intergenic
CAGCTCTTCTTTGGAAGAAA+TGG 0.365649 1.3:+802032 MS.gene64028:CDS
AAAGGCCCTCCAGAGAAATT+AGG 0.368746 1.3:+800676 MS.gene64028:CDS
ATTGGTGCTCCTAATTTCTC+TGG 0.369387 1.3:-800685 None:intergenic
TATTGCAGGAGCATCTGATT+TGG 0.372446 1.3:-802204 None:intergenic
TGAGATTCTTATCAATCATA+TGG 0.372514 1.3:-800273 None:intergenic
CAAATTTGGTCTTTGCCAAA+TGG 0.377269 1.3:+802275 MS.gene64028:CDS
GGAAACTTTGCTCTGTTTGA+AGG 0.378573 1.3:-801172 None:intergenic
TCAATAATGTTGGGGCTTGT+CGG 0.380965 1.3:-801027 None:intergenic
GAACTCTTGTCAATAATGTT+GGG 0.387120 1.3:-801036 None:intergenic
GCCAGTTGTCTTCAGTGAAC+GGG 0.388066 1.3:+800305 MS.gene64028:CDS
GATGAACTGGCAAAACAAAT+TGG 0.391064 1.3:+801876 MS.gene64028:CDS
CAGGAGCTGGTGGCGTTTCA+GGG 0.395704 1.3:+802336 MS.gene64028:CDS
TCTTTGAAACTTCCTTGTTT+CGG 0.396161 1.3:-800592 None:intergenic
AGCTGGTGGCGTTTCAGGGA+TGG 0.404802 1.3:+802340 MS.gene64028:CDS
CTCCGGTAGTACCTCAAGTT+GGG 0.406048 1.3:+802495 MS.gene64028:CDS
GACCGTGGTTCATTGATAGA+TGG 0.406642 1.3:+799990 MS.gene64028:CDS
AGATGCTAAGTCTCATGATA+TGG 0.410271 1.3:+800234 MS.gene64028:CDS
TCATGTTCCAAAGAAGATTC+AGG 0.415078 1.3:-800781 None:intergenic
AAGTCTCATGATATGGATAC+TGG 0.415945 1.3:+800241 MS.gene64028:CDS
GAATTAGATTTGAATAGTAT+TGG 0.417428 1.3:+801966 MS.gene64028:CDS
GCTGGTGGCGTTTCAGGGAT+GGG 0.419252 1.3:+802341 MS.gene64028:CDS
AGGTATCGGTGTTGTAGATA+TGG 0.420582 1.3:-801625 None:intergenic
GAGCAGGAACTAAATGATAA+AGG 0.420968 1.3:+800658 MS.gene64028:CDS
AAACTTCCTTGTTTCGGTAC+AGG 0.423549 1.3:-800586 None:intergenic
TTCAGCGGAGTTGCTTTGTC+TGG 0.423774 1.3:-801412 None:intergenic
CCTGAAACGCCACCAGCTCC+TGG 0.426886 1.3:-802335 None:intergenic
GGTACACAGCTTGGTTTAAA+CGG 0.427299 1.3:+802578 MS.gene64028:CDS
CCTCTTGAGTCCACCATGTA+AGG 0.431827 1.3:-802470 None:intergenic
CCATCCTCTGATCTGAAGGT+TGG 0.433939 1.3:-802004 None:intergenic
AACTGCGAAGACTCCAAGTC+AGG 0.434097 1.3:-801498 None:intergenic
TCGCCAGCCGCGTATGGATC+AGG 0.434737 1.3:+802437 MS.gene64028:CDS
CATCCTCTGATCTGAAGGTT+GGG 0.434962 1.3:-802003 None:intergenic
CGTCGCCGTGATTAGAAACT+TGG 0.436512 1.3:-800963 None:intergenic
GCACCAACAGAAACAGATTC+AGG 0.440066 1.3:-800718 None:intergenic
ACTTTGACTGAGATGAAAGA+TGG 0.442066 1.3:+799800 MS.gene64028:CDS
GCCACTGTAAGTAACCTTCT+TGG 0.442723 1.3:+800142 MS.gene64028:CDS
AGAACTCTTGTCAATAATGT+TGG 0.444898 1.3:-801037 None:intergenic
CAGCCATGCTCATAATATAT+GGG 0.450624 1.3:-802556 None:intergenic
CACAACCCCTTCTCGAGTGC+AGG 0.453974 1.3:+799826 MS.gene64028:CDS
TTTGACAAGTGGAAAGAAGT+TGG 0.454663 1.3:+800205 MS.gene64028:CDS
ACAGAAACAGATTCAGGCTT+AGG 0.456277 1.3:-800712 None:intergenic
GGGGCTGAGCGTAAGATGAT+AGG 0.457202 1.3:-802535 None:intergenic
AGAACAGAGCTATGGAAGAT+AGG 0.458167 1.3:+802702 MS.gene64028:CDS
AAGTGGAAAGAAGTTGGAAA+TGG 0.459088 1.3:+800211 MS.gene64028:CDS
CCCCGTTCACTGAAGACAAC+TGG 0.460330 1.3:-800306 None:intergenic
GCCAGAATGCATCCCTGACT+TGG 0.466600 1.3:+801485 MS.gene64028:CDS
GAAGGAATCAGAACTATTTG+AGG 0.468412 1.3:-801154 None:intergenic
TCCCAACTTTGATCTTAACT+CGG 0.470352 1.3:+802616 MS.gene64028:CDS
ACCCGAGTTAAGATCAAAGT+TGG 0.470545 1.3:-802618 None:intergenic
TGCTGATGTTTGTAGCCAAA+TGG 0.472264 1.3:-802800 None:intergenic
GGTTGGGAAACATATCCATT+TGG 0.474328 1.3:+802785 MS.gene64028:CDS
CTACAGTTTGAAGGAACGCT+TGG 0.475273 1.3:+801699 MS.gene64028:CDS
ACAGCCCCTCTACAGTTTGA+AGG 0.475325 1.3:+801690 MS.gene64028:CDS
GTTTCCCAACCGCTACCATC+TGG 0.477770 1.3:-802773 None:intergenic
TTCAACAATCCTCAAGTTCA+GGG 0.479017 1.3:+802729 MS.gene64028:CDS
ACTGAAGCTTTAGATAGTTG+TGG 0.480850 1.3:-800922 None:intergenic
ATTGCTAGAATCTGTGGCTC+TGG 0.481896 1.3:-800899 None:intergenic
CCATATATTATGAGCATGGC+TGG 0.483020 1.3:+802557 MS.gene64028:CDS
AAAAGAATCAGAGCCATTTG+AGG 0.484474 1.3:-800989 None:intergenic
AGTAAATCAGTTTGAAAGTA+CGG 0.485029 1.3:-802131 None:intergenic
TTGAATAGTATTGGTGATGA+TGG 0.487137 1.3:+801975 MS.gene64028:CDS
TCTTGCTCTATCTTGAACCT+TGG 0.489350 1.3:-800176 None:intergenic
GTTCATGTGTTGCGACTTGA+AGG 0.489465 1.3:+800343 MS.gene64028:CDS
AGTGAGAAGCTAATTTCATC+AGG 0.491477 1.3:+800112 MS.gene64028:CDS
TTTGGCAAAGACCAAATTTG+AGG 0.492121 1.3:-802272 None:intergenic
CTGAACTTGAGGATTGTTGA+AGG 0.492765 1.3:-802727 None:intergenic
CCTTCCATCCTCTGATCTGA+AGG 0.493211 1.3:-802008 None:intergenic
ACTGAAGCAGCTCAAGATCC+AGG 0.493389 1.3:+801522 MS.gene64028:CDS
CTTATCAATGAAGGAGGAGC+AGG 0.493727 1.3:+800642 MS.gene64028:CDS
AAGAAGATTTGGTAGGCCCT+TGG 0.495044 1.3:-802157 None:intergenic
GGCACTATTGGTGCAGGCAC+AGG 0.495570 1.3:-800760 None:intergenic
CAGCTTGGTTTAAACGGTGT+TGG 0.495798 1.3:+802584 MS.gene64028:CDS
AGCATGGCTGGTACACAGCT+TGG 0.499968 1.3:+802569 MS.gene64028:CDS
CTTTGTCTGGATGACACCTC+GGG 0.502499 1.3:-801399 None:intergenic
TCCACTGATGATTGCCCAGA+AGG 0.506454 1.3:-801915 None:intergenic
CAGAAGAATGTTTCTGCTGG+TGG 0.507286 1.3:+801786 MS.gene64028:CDS
GGCAATCCTGAAGTCTGTGC+AGG 0.508998 1.3:-801666 None:intergenic
CTTTGACTGAGATGAAAGAT+GGG 0.510506 1.3:+799801 MS.gene64028:CDS
GCCACCAACCCCTGAACTTG+AGG 0.517663 1.3:-802738 None:intergenic
GTTAAAAGACTTATCAATGA+AGG 0.520916 1.3:+800633 MS.gene64028:CDS
CCCAACTTTGATCTTAACTC+GGG 0.520919 1.3:+802617 MS.gene64028:CDS
CGGGTTTCATGATTGATGGC+GGG 0.521501 1.3:+802636 MS.gene64028:CDS
TGGCGATGCAATAAGACAAT+GGG 0.521998 1.3:+799898 MS.gene64028:CDS
GCGTTCCTTCAAACTGTAGA+GGG 0.522910 1.3:-801695 None:intergenic
CCACCTGATCCATACGCGGC+TGG 0.523562 1.3:-802440 None:intergenic
AAGCCTGAATCTGTTTCTGT+TGG 0.524255 1.3:+800715 MS.gene64028:CDS
CCAACTCCTGCACTCGAGAA+GGG 0.524276 1.3:-799832 None:intergenic
GGTGCTCCTAATTTCTCTGG+AGG 0.525103 1.3:-800682 None:intergenic
GATGTCAAAAGCAGTGAAGT+TGG 0.525752 1.3:+800802 MS.gene64028:CDS
TTGGTCTTTGCCAAATGGCA+AGG 0.528902 1.3:+802280 MS.gene64028:CDS
AAGGAACGCTTGGATGGAAA+GGG 0.529359 1.3:+801709 MS.gene64028:CDS
GTAAGATGATAGGACATTTG+AGG 0.529359 1.3:-802525 None:intergenic
GAAGCAGAGACAACAGGTAT+CGG 0.532277 1.3:-801639 None:intergenic
ACATTCATATGCCTTTCCTC+AGG 0.532717 1.3:-801441 None:intergenic
GAAGGTGGAACGAGGCAAGC+TGG 0.534105 1.3:+801330 MS.gene64028:CDS
CACGGCGACGATACCTCAAA+TGG 0.534538 1.3:+800976 MS.gene64028:CDS
GCTCCGGTAGTACCTCAAGT+TGG 0.535810 1.3:+802494 MS.gene64028:CDS
TCCTCAAGTTCAGGGGTTGG+TGG 0.537669 1.3:+802737 MS.gene64028:CDS
TGATGCAGGACGAAAGGCAC+TGG 0.538116 1.3:-801742 None:intergenic
ACGAAAGGCACTGGTGGCAG+CGG 0.538807 1.3:-801733 None:intergenic
GAAGGAACGCTTGGATGGAA+AGG 0.538921 1.3:+801708 MS.gene64028:CDS
ACCAACTCCTGCACTCGAGA+AGG 0.540838 1.3:-799833 None:intergenic
AGAACCAGATGGTAGCGGTT+GGG 0.543187 1.3:+802769 MS.gene64028:CDS
GGTGGACTCAAGAGGAGCTC+CGG 0.543800 1.3:+802478 MS.gene64028:CDS
CCCTTCTCGAGTGCAGGAGT+TGG 0.544889 1.3:+799832 MS.gene64028:CDS
CCTTCAGATCAGAGGATGGA+AGG 0.544949 1.3:+802008 MS.gene64028:CDS
GAATGATTGTAGGATACCGC+AGG 0.545072 1.3:-802365 None:intergenic
GTGCTCCTAATTTCTCTGGA+GGG 0.547044 1.3:-800681 None:intergenic
GCCTTCTGGGCAATCATCAG+TGG 0.549027 1.3:+801914 MS.gene64028:CDS
GACGATGATGATGCAGGTGA+TGG 0.549546 1.3:-802065 None:intergenic
AGCTAATTTCATCAGGAATG+TGG 0.552057 1.3:+800119 MS.gene64028:CDS
GATTCAGGCACTATTGGTGC+AGG 0.552270 1.3:-800766 None:intergenic
GCTTTGTCTGGATGACACCT+CGG 0.553283 1.3:-801400 None:intergenic
AGTTTGAAGGAACGCTTGGA+TGG 0.554006 1.3:+801703 MS.gene64028:CDS
AACTCTTGTCAATAATGTTG+GGG 0.554203 1.3:-801035 None:intergenic
GGCCAGTTGTCTTCAGTGAA+CGG 0.554883 1.3:+800304 MS.gene64028:CDS
AAACTGATTTACTGGACCAA+GGG 0.555008 1.3:+802141 MS.gene64028:CDS
TGTCAATTCACTTTCACTTG+AGG 0.555058 1.3:-800542 None:intergenic
CCCGAGTTAAGATCAAAGTT+GGG 0.555258 1.3:-802617 None:intergenic
AGGTGGTACAGTTCCTTACA+TGG 0.559099 1.3:+802457 MS.gene64028:CDS
AGATACTCAGATACCAGATA+AGG 0.560646 1.3:+800393 MS.gene64028:CDS
TTCCTCAGGACATGCTTCAG+CGG 0.561075 1.3:-801427 None:intergenic
AAGTCAAAACAGTAGTAAGA+TGG 0.561949 1.3:-800501 None:intergenic
AAATCAATGTTTCGAACTGA+CGG 0.562322 1.3:-802098 None:intergenic
AAAGCTTCAGTGGAGGAAGA+AGG 0.562681 1.3:+800934 MS.gene64028:CDS
TCTGGATGACACCTCGGGTA+GGG 0.563962 1.3:-801394 None:intergenic
CTTACAATGATGCCAGGAGC+TGG 0.565275 1.3:+802323 MS.gene64028:CDS
TCTTCAGAGAAAAGCTCAGA+AGG 0.565440 1.3:+801312 MS.gene64028:CDS
AAGAACCAGATGGTAGCGGT+TGG 0.566058 1.3:+802768 MS.gene64028:CDS
GTTAAGATCAAAGTTGGGAC+GGG 0.566742 1.3:-802612 None:intergenic
CATTAAGATCAAAGGTGCAA+AGG 0.568125 1.3:-801563 None:intergenic
TCCGGTAGTACCTCAAGTTG+GGG 0.568757 1.3:+802496 MS.gene64028:CDS
GCTAATTTCATCAGGAATGT+GGG 0.570539 1.3:+800120 MS.gene64028:CDS
ACAACTATCTAAAGCTTCAG+TGG 0.571210 1.3:+800924 MS.gene64028:CDS
AACAGCCAAGTTTCTAATCA+CGG 0.572080 1.3:+800958 MS.gene64028:CDS
ATAGAGATGCATTGACTGCA+AGG 0.572735 1.3:+802660 MS.gene64028:CDS
TCCTGGTCAGAACAGAGCTA+TGG 0.573956 1.3:+802694 MS.gene64028:CDS
TTCACAATTCAAACTTGCAG+AGG 0.574128 1.3:-800458 None:intergenic
ATACTTGAAGAAGATTTGGT+AGG 0.576252 1.3:-802164 None:intergenic
ATATTCTTCATTAAGATCAA+AGG 0.576467 1.3:-801571 None:intergenic
CTGAAGCATGTCCTGAGGAA+AGG 0.576650 1.3:+801430 MS.gene64028:CDS
CAACTCCTGCACTCGAGAAG+GGG 0.576939 1.3:-799831 None:intergenic
TCTTGGTCATCACAGCTCCA+AGG 0.578227 1.3:+800159 MS.gene64028:CDS
GTTGGTCTCTGTGATGAAGA+AGG 0.579660 1.3:+799850 MS.gene64028:CDS
CAATCCTCAAGTTCAGGGGT+TGG 0.580162 1.3:+802734 MS.gene64028:CDS
CAAGAGCACTATTTGACAAG+TGG 0.581259 1.3:+800194 MS.gene64028:CDS
ACGAGGTGATGCAGGACGAA+AGG 0.582185 1.3:-801748 None:intergenic
GTAGATCTTACAATGATGCC+AGG 0.582215 1.3:+802317 MS.gene64028:CDS
ATCATTGTAAGATCTACTGC+AGG 0.583726 1.3:-802311 None:intergenic
AGCCATGCTCATAATATATG+GGG 0.584863 1.3:-802555 None:intergenic
TCCATAGCTCTGTTCTGACC+AGG 0.588974 1.3:-802695 None:intergenic
GATAAGAATCTCAAGGAGGA+AGG 0.590458 1.3:+800283 MS.gene64028:CDS
AGAAGAATGTTTCTGCTGGT+GGG 0.590816 1.3:+801787 MS.gene64028:CDS
CAAACTGATTTACTGGACCA+AGG 0.591390 1.3:+802140 MS.gene64028:CDS
AACTTACCTGTACCGAAACA+AGG 0.592075 1.3:+800580 MS.gene64028:CDS
TGAACTTGAGGATTGTTGAA+GGG 0.595658 1.3:-802726 None:intergenic
TCAAACTGTAGAGGGGCTGT+TGG 0.595682 1.3:-801687 None:intergenic
TCATGTGTTGCGACTTGAAG+GGG 0.596486 1.3:+800345 MS.gene64028:CDS
AGATCAAAGTTGGGACGGGA+AGG 0.596681 1.3:-802608 None:intergenic
AGTTAAGATCAAAGTTGGGA+CGG 0.597365 1.3:-802613 None:intergenic
CTGGCGATGCAATAAGACAA+TGG 0.600185 1.3:+799897 MS.gene64028:CDS
CTTCAGATCAGAGGATGGAA+GGG 0.600286 1.3:+802009 MS.gene64028:CDS
GCTGGCAGCGTGGAATCTGT+TGG 0.600434 1.3:+801348 MS.gene64028:CDS
GACAGTATTGTGAAGAACAC+TGG 0.602791 1.3:+799878 MS.gene64028:CDS
ACTGCGAAGACTCCAAGTCA+GGG 0.608574 1.3:-801497 None:intergenic
TTGTGGATTGCTAGAATCTG+TGG 0.608879 1.3:-800905 None:intergenic
ATTGACGACGATGATGATGC+AGG 0.609742 1.3:-802071 None:intergenic
CCTTACATGGTGGACTCAAG+AGG 0.614111 1.3:+802470 MS.gene64028:CDS
TCGGGTTTCATGATTGATGG+CGG 0.615028 1.3:+802635 MS.gene64028:CDS
AAAAGACTTATCAATGAAGG+AGG 0.617401 1.3:+800636 MS.gene64028:CDS
TATGATTGATAAGAATCTCA+AGG 0.619192 1.3:+800276 MS.gene64028:CDS
AATCAGAAGAATGTTTCTGC+TGG 0.619736 1.3:+801783 MS.gene64028:CDS
ACGATGATGATGCAGGTGAT+GGG 0.620906 1.3:-802064 None:intergenic
TCAGAGAAAAGCTCAGAAGG+TGG 0.622813 1.3:+801315 MS.gene64028:CDS
AGCGTTCCTTCAAACTGTAG+AGG 0.623038 1.3:-801696 None:intergenic
ACAATGATGCCAGGAGCTGG+TGG 0.623886 1.3:+802326 MS.gene64028:CDS
GTCTGGATGACACCTCGGGT+AGG 0.626282 1.3:-801395 None:intergenic
TATTGACAAGAGTTCTGACA+TGG 0.626964 1.3:+801044 MS.gene64028:CDS
AGAAAAGAACCAGATGGTAG+CGG 0.632014 1.3:+802764 MS.gene64028:CDS
TGCAGGACGAAAGGCACTGG+TGG 0.633312 1.3:-801739 None:intergenic
CCAACCTTCAGATCAGAGGA+TGG 0.635017 1.3:+802004 MS.gene64028:CDS
CTCTGATAAAATGAGTGTGG+TGG 0.638162 1.3:+800870 MS.gene64028:CDS
GATAAAATGAGTGTGGTGGA+TGG 0.638209 1.3:+800874 MS.gene64028:CDS
AAAGTGAATTGACATCGTCT+GGG 0.640193 1.3:+800551 MS.gene64028:CDS
ACCAAGAAGGTTACTTACAG+TGG 0.640251 1.3:-800143 None:intergenic
TTCATGTGTTGCGACTTGAA+GGG 0.641389 1.3:+800344 MS.gene64028:CDS
ACTTCCTCAGCAACTTGTCG+AGG 0.645833 1.3:-801102 None:intergenic
CCTTGCATGTTCCCTACCCG+AGG 0.646133 1.3:+801383 MS.gene64028:CDS
ACTCTGATATATCCAAACAA+AGG 0.646226 1.3:+801811 MS.gene64028:CDS
TGGTACAAATGATAGTGTCA+TGG 0.647688 1.3:+800039 MS.gene64028:CDS
TTTGATCTGAATGTGGCTGG+GGG 0.649877 1.3:+801849 MS.gene64028:CDS
TCAACAATCCTCAAGTTCAG+GGG 0.649996 1.3:+802730 MS.gene64028:CDS
CTCCGCTGAAGCATGTCCTG+AGG 0.650146 1.3:+801425 MS.gene64028:CDS
AATTGAACTTGACTATGGCA+TGG 0.651982 1.3:+801227 MS.gene64028:CDS
TGGAGCTGTGATGACCAAGA+AGG 0.654122 1.3:-800156 None:intergenic
GGCAAAAGAAAAGAACCAGA+TGG 0.656215 1.3:+802758 MS.gene64028:CDS
CCAGTTGTCTTCAGTGAACG+GGG 0.656595 1.3:+800306 MS.gene64028:CDS
TTATTCAATTAGAAGGACCG+TGG 0.658634 1.3:+799975 MS.gene64028:CDS
ATACCCAACCTTCAGATCAG+AGG 0.660327 1.3:+802000 MS.gene64028:CDS
AACATCAGCAACAACCTCCG+TGG 0.660346 1.3:+802813 MS.gene64028:CDS
AACGAGGCAAGCTGGCAGCG+TGG 0.660760 1.3:+801338 MS.gene64028:CDS
ATATATTAGAGCTACTTCCA+CGG 0.662581 1.3:-802830 None:intergenic
ACTATCTAAAGCTTCAGTGG+AGG 0.665990 1.3:+800927 MS.gene64028:CDS
CCAGCCGCGTATGGATCAGG+TGG 0.671996 1.3:+802440 MS.gene64028:CDS
CGTTTCAGGGATGGGCCCTG+CGG 0.673116 1.3:+802349 MS.gene64028:CDS
AATGATTGTAGGATACCGCA+GGG 0.677137 1.3:-802364 None:intergenic
CGTTCCTTCAAACTGTAGAG+GGG 0.681833 1.3:-801694 None:intergenic
CATGTGTTGCGACTTGAAGG+GGG 0.682268 1.3:+800346 MS.gene64028:CDS
TGTTCCTCGACAAGTTGCTG+AGG 0.683361 1.3:+801098 MS.gene64028:CDS
GCCATGCTCATAATATATGG+GGG 0.686789 1.3:-802554 None:intergenic
TGCCTCTGATAAAATGAGTG+TGG 0.687179 1.3:+800867 MS.gene64028:CDS
CCTCGGGTAGGGAACATGCA+AGG 0.689926 1.3:-801383 None:intergenic
AGTCGCTAGACAAGTTGCTG+AGG 0.692831 1.3:+801263 MS.gene64028:CDS
TGTACCACCTGATCCATACG+CGG 0.695609 1.3:-802444 None:intergenic
AAAGCTCAGAAGGTGGAACG+AGG 0.698086 1.3:+801322 MS.gene64028:CDS
AACCATCTATCAATGAACCA+CGG 0.715390 1.3:-799992 None:intergenic
TGGTACAGTTCCTTACATGG+TGG 0.722506 1.3:+802460 MS.gene64028:CDS
CCGGTAGTACCTCAAGTTGG+GGG 0.731940 1.3:+802497 MS.gene64028:CDS
GATTGATAAGAATCTCAAGG+AGG 0.757896 1.3:+800279 MS.gene64028:CDS
TATTAGAGCTACTTCCACGG+AGG 0.760756 1.3:-802827 None:intergenic
GAAGCAGCTCAAGATCCAGG+AGG 0.773089 1.3:+801525 MS.gene64028:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTCTTCATTAAGATCAA+AGG - chr1.3:801574-801593 None:intergenic 20.0%
!! GAATTAGATTTGAATAGTAT+TGG + chr1.3:801966-801985 MS.gene64028:CDS 20.0%
!! TTAAATGATGCTCAAAAATT+GGG + chr1.3:800013-800032 MS.gene64028:CDS 20.0%
!! TTTGATCTTAATGAAGAATA+TGG + chr1.3:801573-801592 MS.gene64028:CDS 20.0%
!!! TACAATCATTCAACTTTTTT+GGG + chr1.3:802377-802396 MS.gene64028:CDS 20.0%
! AAGTTCAATTTCAGAATTCT+TGG - chr1.3:801217-801236 None:intergenic 25.0%
! AGTAAATCAGTTTGAAAGTA+CGG - chr1.3:802134-802153 None:intergenic 25.0%
! CAAAAAAGTTGAATGATTGT+AGG - chr1.3:802378-802397 None:intergenic 25.0%
! CTAATTGAATAAAAAGGTCA+AGG - chr1.3:799967-799986 None:intergenic 25.0%
! GTCCTTCTAATTGAATAAAA+AGG - chr1.3:799973-799992 None:intergenic 25.0%
! GTTAAAAGACTTATCAATGA+AGG + chr1.3:800633-800652 MS.gene64028:CDS 25.0%
! GTTAAATGATGCTCAAAAAT+TGG + chr1.3:800012-800031 MS.gene64028:CDS 25.0%
! TATGATTGATAAGAATCTCA+AGG + chr1.3:800276-800295 MS.gene64028:CDS 25.0%
! TGAGATTCTTATCAATCATA+TGG - chr1.3:800276-800295 None:intergenic 25.0%
! TGTGATAAAGTAATAGTTTC+TGG + chr1.3:800829-800848 MS.gene64028:CDS 25.0%
! TTCAATACTTGAAGAAGATT+TGG - chr1.3:802171-802190 None:intergenic 25.0%
!! CAAATCTCTTTGATCTTTTA+GGG - chr1.3:801947-801966 None:intergenic 25.0%
!! GACCTTTTTATTCAATTAGA+AGG + chr1.3:799968-799987 MS.gene64028:CDS 25.0%
!! TCAAATCTCTTTGATCTTTT+AGG - chr1.3:801948-801967 None:intergenic 25.0%
!!! CTACAATCATTCAACTTTTT+TGG + chr1.3:802376-802395 MS.gene64028:CDS 25.0%
!!! TCAACTTTTTTGGGATATAA+TGG + chr1.3:802386-802405 MS.gene64028:CDS 25.0%
!!! TCTTCAAGTATTGAAGTATA+TGG + chr1.3:802176-802195 MS.gene64028:CDS 25.0%
!!! TCTTTTAACTGTAAATCATC+AGG - chr1.3:800622-800641 None:intergenic 25.0%
AAAAGACTTATCAATGAAGG+AGG + chr1.3:800636-800655 MS.gene64028:CDS 30.0%
AAACAAATTGGTGAATCTTC+AGG + chr1.3:801888-801907 MS.gene64028:CDS 30.0%
AAATCAATGTTTCGAACTGA+CGG - chr1.3:802101-802120 None:intergenic 30.0%
AACTCTTGTCAATAATGTTG+GGG - chr1.3:801038-801057 None:intergenic 30.0%
AAGAAAATCTTGCCTTTGTT+TGG - chr1.3:801826-801845 None:intergenic 30.0%
AAGTCAAAACAGTAGTAAGA+TGG - chr1.3:800504-800523 None:intergenic 30.0%
ACTCTGATATATCCAAACAA+AGG + chr1.3:801811-801830 MS.gene64028:CDS 30.0%
AGAACTCTTGTCAATAATGT+TGG - chr1.3:801040-801059 None:intergenic 30.0%
GAACTCTTGTCAATAATGTT+GGG - chr1.3:801039-801058 None:intergenic 30.0%
! ATACTTGAAGAAGATTTGGT+AGG - chr1.3:802167-802186 None:intergenic 30.0%
! GTACTTTCAAACTGATTTAC+TGG + chr1.3:802133-802152 MS.gene64028:CDS 30.0%
! TCTGAAATTGAACTTGACTA+TGG + chr1.3:801222-801241 MS.gene64028:CDS 30.0%
! TGATTATCAGCATTTTTACG+AGG - chr1.3:801768-801787 None:intergenic 30.0%
!! TCTTGATTTTGATCTGAATG+TGG + chr1.3:801842-801861 MS.gene64028:CDS 30.0%
!! TCTTTGAAACTTCCTTGTTT+CGG - chr1.3:800595-800614 None:intergenic 30.0%
!! TTGAATAGTATTGGTGATGA+TGG + chr1.3:801975-801994 MS.gene64028:CDS 30.0%
AAAAGAATCAGAGCCATTTG+AGG - chr1.3:800992-801011 None:intergenic 35.0%
AAAGAAGATTCAGGCACTAT+TGG - chr1.3:800775-800794 None:intergenic 35.0%
AAAGTGAATTGACATCGTCT+GGG + chr1.3:800551-800570 MS.gene64028:CDS 35.0%
AACAGCCAAGTTTCTAATCA+CGG + chr1.3:800958-800977 MS.gene64028:CDS 35.0%
AACCATCTATCAATGAACCA+CGG - chr1.3:799995-800014 None:intergenic 35.0%
AAGTCTCATGATATGGATAC+TGG + chr1.3:800241-800260 MS.gene64028:CDS 35.0%
AAGTGGAAAGAAGTTGGAAA+TGG + chr1.3:800211-800230 MS.gene64028:CDS 35.0%
AATAGTGCCTGAATCTTCTT+TGG + chr1.3:800774-800793 MS.gene64028:CDS 35.0%
AATGATGCTCAAAAATTGGG+TGG + chr1.3:800016-800035 MS.gene64028:CDS 35.0%
AATTGAACTTGACTATGGCA+TGG + chr1.3:801227-801246 MS.gene64028:CDS 35.0%
ACTTTGACTGAGATGAAAGA+TGG + chr1.3:799800-799819 MS.gene64028:CDS 35.0%
AGATACTCAGATACCAGATA+AGG + chr1.3:800393-800412 MS.gene64028:CDS 35.0%
AGATGCTAAGTCTCATGATA+TGG + chr1.3:800234-800253 MS.gene64028:CDS 35.0%
AGCCATGCTCATAATATATG+GGG - chr1.3:802558-802577 None:intergenic 35.0%
AGCTAATTTCATCAGGAATG+TGG + chr1.3:800119-800138 MS.gene64028:CDS 35.0%
AGTGAGAAGCTAATTTCATC+AGG + chr1.3:800112-800131 MS.gene64028:CDS 35.0%
AGTTAAGATCAAAGTTGGGA+CGG - chr1.3:802616-802635 None:intergenic 35.0%
ATCATTGTAAGATCTACTGC+AGG - chr1.3:802314-802333 None:intergenic 35.0%
CAGCCATGCTCATAATATAT+GGG - chr1.3:802559-802578 None:intergenic 35.0%
CTTTGACTGAGATGAAAGAT+GGG + chr1.3:799801-799820 MS.gene64028:CDS 35.0%
GAAGGAATCAGAACTATTTG+AGG - chr1.3:801157-801176 None:intergenic 35.0%
GAGCAGGAACTAAATGATAA+AGG + chr1.3:800658-800677 MS.gene64028:CDS 35.0%
GATGAACTGGCAAAACAAAT+TGG + chr1.3:801876-801895 MS.gene64028:CDS 35.0%
GATTCAGGCTTAGGATTTAT+TGG - chr1.3:800706-800725 None:intergenic 35.0%
GATTGATAAGAATCTCAAGG+AGG + chr1.3:800279-800298 MS.gene64028:CDS 35.0%
GCTAATTTCATCAGGAATGT+GGG + chr1.3:800120-800139 MS.gene64028:CDS 35.0%
GTAAGATGATAGGACATTTG+AGG - chr1.3:802528-802547 None:intergenic 35.0%
TCATGTTCCAAAGAAGATTC+AGG - chr1.3:800784-800803 None:intergenic 35.0%
TCCCAACTTTGATCTTAACT+CGG + chr1.3:802616-802635 MS.gene64028:CDS 35.0%
TCTGACATGGAACTTGATTA+TGG + chr1.3:801057-801076 MS.gene64028:CDS 35.0%
TGAACTTGAGGATTGTTGAA+GGG - chr1.3:802729-802748 None:intergenic 35.0%
TGTCAATTCACTTTCACTTG+AGG - chr1.3:800545-800564 None:intergenic 35.0%
TTATTCAATTAGAAGGACCG+TGG + chr1.3:799975-799994 MS.gene64028:CDS 35.0%
TTCAACAATCCTCAAGTTCA+GGG + chr1.3:802729-802748 MS.gene64028:CDS 35.0%
TTCACAATTCAAACTTGCAG+AGG - chr1.3:800461-800480 None:intergenic 35.0%
TTCTGACCAGGAAAGAAAAA+CGG - chr1.3:802686-802705 None:intergenic 35.0%
TTTGACAAGTGGAAAGAAGT+TGG + chr1.3:800205-800224 MS.gene64028:CDS 35.0%
TTTGGCAAAGACCAAATTTG+AGG - chr1.3:802275-802294 None:intergenic 35.0%
! AAACTGATTTACTGGACCAA+GGG + chr1.3:802141-802160 MS.gene64028:CDS 35.0%
! AAGAGCATGTTCCTCAAATT+TGG + chr1.3:802261-802280 MS.gene64028:CDS 35.0%
! AATCAGAAGAATGTTTCTGC+TGG + chr1.3:801783-801802 MS.gene64028:CDS 35.0%
! ACAACTATCTAAAGCTTCAG+TGG + chr1.3:800924-800943 MS.gene64028:CDS 35.0%
! CAAATTTGGTCTTTGCCAAA+TGG + chr1.3:802275-802294 MS.gene64028:CDS 35.0%
! CATTAAGATCAAAGGTGCAA+AGG - chr1.3:801566-801585 None:intergenic 35.0%
! TATTGACAAGAGTTCTGACA+TGG + chr1.3:801044-801063 MS.gene64028:CDS 35.0%
! TCTTTGGAAGAAATGGCTAT+TGG + chr1.3:802039-802058 MS.gene64028:CDS 35.0%
! TGGTACAAATGATAGTGTCA+TGG + chr1.3:800039-800058 MS.gene64028:CDS 35.0%
! TTAACATTAGCAGCCTTATC+TGG - chr1.3:800409-800428 None:intergenic 35.0%
!! ACTGAAGCTTTAGATAGTTG+TGG - chr1.3:800925-800944 None:intergenic 35.0%
!! ATTTTGATCTGAATGTGGCT+GGG + chr1.3:801847-801866 MS.gene64028:CDS 35.0%
AACTCGGGTTTCATGATTGA+TGG + chr1.3:802632-802651 MS.gene64028:CDS 40.0%
AACTTACCTGTACCGAAACA+AGG + chr1.3:800580-800599 MS.gene64028:CDS 40.0%
AATGATTGTAGGATACCGCA+GGG - chr1.3:802367-802386 None:intergenic 40.0%
ACAGAAACAGATTCAGGCTT+AGG - chr1.3:800715-800734 None:intergenic 40.0%
ACATTCATATGCCTTTCCTC+AGG - chr1.3:801444-801463 None:intergenic 40.0%
ACCAAGAAGGTTACTTACAG+TGG - chr1.3:800146-800165 None:intergenic 40.0%
ACCCGAGTTAAGATCAAAGT+TGG - chr1.3:802621-802640 None:intergenic 40.0%
AGAAAAGAACCAGATGGTAG+CGG + chr1.3:802764-802783 MS.gene64028:CDS 40.0%
AGAACAGAGCTATGGAAGAT+AGG + chr1.3:802702-802721 MS.gene64028:CDS 40.0%
CCAGCCATGCTCATAATATA+TGG - chr1.3:802560-802579 None:intergenic 40.0%
CCATATATTATGAGCATGGC+TGG + chr1.3:802557-802576 MS.gene64028:CDS 40.0%
CCCAACTTTGATCTTAACTC+GGG + chr1.3:802617-802636 MS.gene64028:CDS 40.0%
CCCGAGTTAAGATCAAAGTT+GGG - chr1.3:802620-802639 None:intergenic 40.0%
CTCTGATAAAATGAGTGTGG+TGG + chr1.3:800870-800889 MS.gene64028:CDS 40.0%
CTGAACTTGAGGATTGTTGA+AGG - chr1.3:802730-802749 None:intergenic 40.0%
CTTCAACAATCCTCAAGTTC+AGG + chr1.3:802728-802747 MS.gene64028:CDS 40.0%
GAAAGTGAATTGACATCGTC+TGG + chr1.3:800550-800569 MS.gene64028:CDS 40.0%
GACAGTATTGTGAAGAACAC+TGG + chr1.3:799878-799897 MS.gene64028:CDS 40.0%
GATAAGAATCTCAAGGAGGA+AGG + chr1.3:800283-800302 MS.gene64028:CDS 40.0%
GATGTCAAAAGCAGTGAAGT+TGG + chr1.3:800802-800821 MS.gene64028:CDS 40.0%
GCCATGCTCATAATATATGG+GGG - chr1.3:802557-802576 None:intergenic 40.0%
GGAAACTTTGCTCTGTTTGA+AGG - chr1.3:801175-801194 None:intergenic 40.0%
GGCAAAAGAAAAGAACCAGA+TGG + chr1.3:802758-802777 MS.gene64028:CDS 40.0%
GGTACACAGCTTGGTTTAAA+CGG + chr1.3:802578-802597 MS.gene64028:CDS 40.0%
GGTTGGGAAACATATCCATT+TGG + chr1.3:802785-802804 MS.gene64028:CDS 40.0%
GTAGATCTTACAATGATGCC+AGG + chr1.3:802317-802336 MS.gene64028:CDS 40.0%
GTTAAGATCAAAGTTGGGAC+GGG - chr1.3:802615-802634 None:intergenic 40.0%
TCAACAATCCTCAAGTTCAG+GGG + chr1.3:802730-802749 MS.gene64028:CDS 40.0%
TCAATAATGTTGGGGCTTGT+CGG - chr1.3:801030-801049 None:intergenic 40.0%
TCTTCAGAGAAAAGCTCAGA+AGG + chr1.3:801312-801331 MS.gene64028:CDS 40.0%
TCTTGCTCTATCTTGAACCT+TGG - chr1.3:800179-800198 None:intergenic 40.0%
TGCCTCTGATAAAATGAGTG+TGG + chr1.3:800867-800886 MS.gene64028:CDS 40.0%
TGCTGATGTTTGTAGCCAAA+TGG - chr1.3:802803-802822 None:intergenic 40.0%
TGGCGATGCAATAAGACAAT+GGG + chr1.3:799898-799917 MS.gene64028:CDS 40.0%
TTCATGTGTTGCGACTTGAA+GGG + chr1.3:800344-800363 MS.gene64028:CDS 40.0%
! AAACTTCCTTGTTTCGGTAC+AGG - chr1.3:800589-800608 None:intergenic 40.0%
! ACACTCATTTTATCAGAGGC+AGG - chr1.3:800868-800887 None:intergenic 40.0%
! ACTATCTAAAGCTTCAGTGG+AGG + chr1.3:800927-800946 MS.gene64028:CDS 40.0%
! AGAAGAATGTTTCTGCTGGT+GGG + chr1.3:801787-801806 MS.gene64028:CDS 40.0%
! ATTGGTGCTCCTAATTTCTC+TGG - chr1.3:800688-800707 None:intergenic 40.0%
! CAAACTGATTTACTGGACCA+AGG + chr1.3:802140-802159 MS.gene64028:CDS 40.0%
! CACCACACTCATTTTATCAG+AGG - chr1.3:800872-800891 None:intergenic 40.0%
! CAGCTCTTCTTTGGAAGAAA+TGG + chr1.3:802032-802051 MS.gene64028:CDS 40.0%
! CTTTTCTCTGAATTACCTCC+TGG - chr1.3:801543-801562 None:intergenic 40.0%
! GATAAAATGAGTGTGGTGGA+TGG + chr1.3:800874-800893 MS.gene64028:CDS 40.0%
! TTGTGGATTGCTAGAATCTG+TGG - chr1.3:800908-800927 None:intergenic 40.0%
! TTTTGATCTGAATGTGGCTG+GGG + chr1.3:801848-801867 MS.gene64028:CDS 40.0%
!! AAGCCTGAATCTGTTTCTGT+TGG + chr1.3:800715-800734 MS.gene64028:CDS 40.0%
!! AGGTATCGGTGTTGTAGATA+TGG - chr1.3:801628-801647 None:intergenic 40.0%
!! ATAGAGATGCATTGACTGCA+AGG + chr1.3:802660-802679 MS.gene64028:CDS 40.0%
!! CAAGAGCACTATTTGACAAG+TGG + chr1.3:800194-800213 MS.gene64028:CDS 40.0%
!! GATTTTGATCTGAATGTGGC+TGG + chr1.3:801846-801865 MS.gene64028:CDS 40.0%
!! TATTGCAGGAGCATCTGATT+TGG - chr1.3:802207-802226 None:intergenic 40.0%
!!! CTCCTGCAATATCGATTTTG+GGG + chr1.3:802216-802235 MS.gene64028:CDS 40.0%
!!! GCTCCTGCAATATCGATTTT+GGG + chr1.3:802215-802234 MS.gene64028:CDS 40.0%
!!! TCCTGCAATATCGATTTTGG+GGG + chr1.3:802217-802236 MS.gene64028:CDS 40.0%
!!! TGCTCCTGCAATATCGATTT+TGG + chr1.3:802214-802233 MS.gene64028:CDS 40.0%
AAAGCTTCAGTGGAGGAAGA+AGG + chr1.3:800934-800953 MS.gene64028:CDS 45.0%
AAAGGCCCTCCAGAGAAATT+AGG + chr1.3:800676-800695 MS.gene64028:CDS 45.0%
AAGGAACGCTTGGATGGAAA+GGG + chr1.3:801709-801728 MS.gene64028:CDS 45.0%
AATCTTCAGGCTTGCCTTCT+GGG + chr1.3:801901-801920 MS.gene64028:CDS 45.0%
AGCGTTCCTTCAAACTGTAG+AGG - chr1.3:801699-801718 None:intergenic 45.0%
AGGTGGTACAGTTCCTTACA+TGG + chr1.3:802457-802476 MS.gene64028:CDS 45.0%
AGTTTGAAGGAACGCTTGGA+TGG + chr1.3:801703-801722 MS.gene64028:CDS 45.0%
ATACCCAACCTTCAGATCAG+AGG + chr1.3:802000-802019 MS.gene64028:CDS 45.0%
ATTGCTAGAATCTGTGGCTC+TGG - chr1.3:800902-800921 None:intergenic 45.0%
CAGCTTGGTTTAAACGGTGT+TGG + chr1.3:802584-802603 MS.gene64028:CDS 45.0%
CATCCTCTGATCTGAAGGTT+GGG - chr1.3:802006-802025 None:intergenic 45.0%
CGTTCCTTCAAACTGTAGAG+GGG - chr1.3:801697-801716 None:intergenic 45.0%
CTACAGTTTGAAGGAACGCT+TGG + chr1.3:801699-801718 MS.gene64028:CDS 45.0%
CTGGCGATGCAATAAGACAA+TGG + chr1.3:799897-799916 MS.gene64028:CDS 45.0%
CTTATCAATGAAGGAGGAGC+AGG + chr1.3:800642-800661 MS.gene64028:CDS 45.0%
CTTCAGATCAGAGGATGGAA+GGG + chr1.3:802009-802028 MS.gene64028:CDS 45.0%
GAAGCAGAGACAACAGGTAT+CGG - chr1.3:801642-801661 None:intergenic 45.0%
GAATGATTGTAGGATACCGC+AGG - chr1.3:802368-802387 None:intergenic 45.0%
GACCGTGGTTCATTGATAGA+TGG + chr1.3:799990-800009 MS.gene64028:CDS 45.0%
GATGCTCAAAAATTGGGTGG+TGG + chr1.3:800019-800038 MS.gene64028:CDS 45.0%
GCACCAACAGAAACAGATTC+AGG - chr1.3:800721-800740 None:intergenic 45.0%
GCCACTGTAAGTAACCTTCT+TGG + chr1.3:800142-800161 MS.gene64028:CDS 45.0%
GCCCCCATATATTATGAGCA+TGG + chr1.3:802553-802572 MS.gene64028:CDS 45.0%
GCGTTCCTTCAAACTGTAGA+GGG - chr1.3:801698-801717 None:intergenic 45.0%
GTGCTCCTAATTTCTCTGGA+GGG - chr1.3:800684-800703 None:intergenic 45.0%
GTTCATGTGTTGCGACTTGA+AGG + chr1.3:800343-800362 MS.gene64028:CDS 45.0%
TCAAAACCTGCACAGACTTC+AGG + chr1.3:801660-801679 MS.gene64028:CDS 45.0%
TCAGAGAAAAGCTCAGAAGG+TGG + chr1.3:801315-801334 MS.gene64028:CDS 45.0%
TCATGTGTTGCGACTTGAAG+GGG + chr1.3:800345-800364 MS.gene64028:CDS 45.0%
TCGGGTTTCATGATTGATGG+CGG + chr1.3:802635-802654 MS.gene64028:CDS 45.0%
TGGTACAGTTCCTTACATGG+TGG + chr1.3:802460-802479 MS.gene64028:CDS 45.0%
TTTGATCTGAATGTGGCTGG+GGG + chr1.3:801849-801868 MS.gene64028:CDS 45.0%
! AAGAAGATTTGGTAGGCCCT+TGG - chr1.3:802160-802179 None:intergenic 45.0%
! ACGATGATGATGCAGGTGAT+GGG - chr1.3:802067-802086 None:intergenic 45.0%
! ATTGACGACGATGATGATGC+AGG - chr1.3:802074-802093 None:intergenic 45.0%
! CAGAAGAATGTTTCTGCTGG+TGG + chr1.3:801786-801805 MS.gene64028:CDS 45.0%
! GCATTTTTACGAGGTGATGC+AGG - chr1.3:801759-801778 None:intergenic 45.0%
! GTTGGTCTCTGTGATGAAGA+AGG + chr1.3:799850-799869 MS.gene64028:CDS 45.0%
! TTGGTCTTTGCCAAATGGCA+AGG + chr1.3:802280-802299 MS.gene64028:CDS 45.0%
!!! AATATCGATTTTGGGGGCCA+AGG + chr1.3:802223-802242 MS.gene64028:CDS 45.0%
!!! GGTTTTGAAGCAGAGACAAC+AGG - chr1.3:801648-801667 None:intergenic 45.0%
!!! TTTTCTACCGACTTCCACCT+TGG - chr1.3:802243-802262 None:intergenic 45.0%
AAAGCTCAGAAGGTGGAACG+AGG + chr1.3:801322-801341 MS.gene64028:CDS 50.0%
AACATCAGCAACAACCTCCG+TGG + chr1.3:802813-802832 MS.gene64028:CDS 50.0%
AACTGCGAAGACTCCAAGTC+AGG - chr1.3:801501-801520 None:intergenic 50.0%
ACAGCCCCTCTACAGTTTGA+AGG + chr1.3:801690-801709 MS.gene64028:CDS 50.0%
ACTGAAGCAGCTCAAGATCC+AGG + chr1.3:801522-801541 MS.gene64028:CDS 50.0%
ACTGCGAAGACTCCAAGTCA+GGG - chr1.3:801500-801519 None:intergenic 50.0%
ACTTCCTCAGCAACTTGTCG+AGG - chr1.3:801105-801124 None:intergenic 50.0%
AGATCAAAGTTGGGACGGGA+AGG - chr1.3:802611-802630 None:intergenic 50.0%
AGTCGCTAGACAAGTTGCTG+AGG + chr1.3:801263-801282 MS.gene64028:CDS 50.0%
ATGGAAGGGCAGCTCTTCTT+TGG + chr1.3:802023-802042 MS.gene64028:CDS 50.0%
CAATCCTCAAGTTCAGGGGT+TGG + chr1.3:802734-802753 MS.gene64028:CDS 50.0%
CATGTGTTGCGACTTGAAGG+GGG + chr1.3:800346-800365 MS.gene64028:CDS 50.0%
CCAACCTTCAGATCAGAGGA+TGG + chr1.3:802004-802023 MS.gene64028:CDS 50.0%
CCAGTTGTCTTCAGTGAACG+GGG + chr1.3:800306-800325 MS.gene64028:CDS 50.0%
CCATCCTCTGATCTGAAGGT+TGG - chr1.3:802007-802026 None:intergenic 50.0%
CCTCTTGAGTCCACCATGTA+AGG - chr1.3:802473-802492 None:intergenic 50.0%
CCTTCAGATCAGAGGATGGA+AGG + chr1.3:802008-802027 MS.gene64028:CDS 50.0%
CCTTCCATCCTCTGATCTGA+AGG - chr1.3:802011-802030 None:intergenic 50.0%
CGGCTGGCGAAAAAGACAAA+GGG - chr1.3:802427-802446 None:intergenic 50.0%
CGTCGCCGTGATTAGAAACT+TGG - chr1.3:800966-800985 None:intergenic 50.0%
CTCCGGTAGTACCTCAAGTT+GGG + chr1.3:802495-802514 MS.gene64028:CDS 50.0%
CTGAAGCATGTCCTGAGGAA+AGG + chr1.3:801430-801449 MS.gene64028:CDS 50.0%
CTTACAATGATGCCAGGAGC+TGG + chr1.3:802323-802342 MS.gene64028:CDS 50.0%
CTTTGTCTGGATGACACCTC+GGG - chr1.3:801402-801421 None:intergenic 50.0%
GAAGGAACGCTTGGATGGAA+AGG + chr1.3:801708-801727 MS.gene64028:CDS 50.0%
GAATCTTCAGGCTTGCCTTC+TGG + chr1.3:801900-801919 MS.gene64028:CDS 50.0%
GATTCAGGCACTATTGGTGC+AGG - chr1.3:800769-800788 None:intergenic 50.0%
GCCAGTTGTCTTCAGTGAAC+GGG + chr1.3:800305-800324 MS.gene64028:CDS 50.0%
GCCCCCAAAATCGATATTGC+AGG - chr1.3:802221-802240 None:intergenic 50.0%
GCTGGCGAAAAAGACAAAGG+GGG - chr1.3:802425-802444 None:intergenic 50.0%
GCTTTGTCTGGATGACACCT+CGG - chr1.3:801403-801422 None:intergenic 50.0%
GGCCAGTTGTCTTCAGTGAA+CGG + chr1.3:800304-800323 MS.gene64028:CDS 50.0%
GGCTGGCGAAAAAGACAAAG+GGG - chr1.3:802426-802445 None:intergenic 50.0%
GGTCACAATGTTAGCGCTTC+TGG - chr1.3:801196-801215 None:intergenic 50.0%
GGTGCTCCTAATTTCTCTGG+AGG - chr1.3:800685-800704 None:intergenic 50.0%
TCAAACTGTAGAGGGGCTGT+TGG - chr1.3:801690-801709 None:intergenic 50.0%
TCCAAGTCAGGGATGCATTC+TGG - chr1.3:801489-801508 None:intergenic 50.0%
TCCACTGATGATTGCCCAGA+AGG - chr1.3:801918-801937 None:intergenic 50.0%
TCCTGGTCAGAACAGAGCTA+TGG + chr1.3:802694-802713 MS.gene64028:CDS 50.0%
TCTTGGTCATCACAGCTCCA+AGG + chr1.3:800159-800178 MS.gene64028:CDS 50.0%
TGGAGCTGTGATGACCAAGA+AGG - chr1.3:800159-800178 None:intergenic 50.0%
TGTACCACCTGATCCATACG+CGG - chr1.3:802447-802466 None:intergenic 50.0%
TGTTCCTCGACAAGTTGCTG+AGG + chr1.3:801098-801117 MS.gene64028:CDS 50.0%
TTCCTCAGGACATGCTTCAG+CGG - chr1.3:801430-801449 None:intergenic 50.0%
! CGGGTTTCATGATTGATGGC+GGG + chr1.3:802636-802655 MS.gene64028:CDS 50.0%
! GACGATGATGATGCAGGTGA+TGG - chr1.3:802068-802087 None:intergenic 50.0%
! TCCATAGCTCTGTTCTGACC+AGG - chr1.3:802698-802717 None:intergenic 50.0%
! TCCGGTAGTACCTCAAGTTG+GGG + chr1.3:802496-802515 MS.gene64028:CDS 50.0%
! TTCAGCGGAGTTGCTTTGTC+TGG - chr1.3:801415-801434 None:intergenic 50.0%
!! AAGAACCAGATGGTAGCGGT+TGG + chr1.3:802768-802787 MS.gene64028:CDS 50.0%
!! AGAACCAGATGGTAGCGGTT+GGG + chr1.3:802769-802788 MS.gene64028:CDS 50.0%
!! CCTTACATGGTGGACTCAAG+AGG + chr1.3:802470-802489 MS.gene64028:CDS 50.0%
!!! GCAAGGCCGTTTTTCTTTCC+TGG + chr1.3:802677-802696 MS.gene64028:CDS 50.0%
ACAATGATGCCAGGAGCTGG+TGG + chr1.3:802326-802345 MS.gene64028:CDS 55.0%
ACCAACTCCTGCACTCGAGA+AGG - chr1.3:799836-799855 None:intergenic 55.0%
ACGAGGTGATGCAGGACGAA+AGG - chr1.3:801751-801770 None:intergenic 55.0%
AGCATGGCTGGTACACAGCT+TGG + chr1.3:802569-802588 MS.gene64028:CDS 55.0%
CAACTCCTGCACTCGAGAAG+GGG - chr1.3:799834-799853 None:intergenic 55.0%
CACGGCGACGATACCTCAAA+TGG + chr1.3:800976-800995 MS.gene64028:CDS 55.0%
CCAACTCCTGCACTCGAGAA+GGG - chr1.3:799835-799854 None:intergenic 55.0%
CCCCGTTCACTGAAGACAAC+TGG - chr1.3:800309-800328 None:intergenic 55.0%
GAAGCAGCTCAAGATCCAGG+AGG + chr1.3:801525-801544 MS.gene64028:CDS 55.0%
GAGGAAGATCCCCCAACTTG+AGG - chr1.3:802509-802528 None:intergenic 55.0%
GCCAGAATGCATCCCTGACT+TGG + chr1.3:801485-801504 MS.gene64028:CDS 55.0%
GCCTTCTGGGCAATCATCAG+TGG + chr1.3:801914-801933 MS.gene64028:CDS 55.0%
GCGGCTGGCGAAAAAGACAA+AGG - chr1.3:802428-802447 None:intergenic 55.0%
GCTCCGGTAGTACCTCAAGT+TGG + chr1.3:802494-802513 MS.gene64028:CDS 55.0%
GGCAATCCTGAAGTCTGTGC+AGG - chr1.3:801669-801688 None:intergenic 55.0%
GGCTCAACAGCCTTGCCATT+TGG - chr1.3:802293-802312 None:intergenic 55.0%
GGGGCTGAGCGTAAGATGAT+AGG - chr1.3:802538-802557 None:intergenic 55.0%
GTTTCCCAACCGCTACCATC+TGG - chr1.3:802776-802795 None:intergenic 55.0%
TCCTCAAGTTCAGGGGTTGG+TGG + chr1.3:802737-802756 MS.gene64028:CDS 55.0%
TCTGGATGACACCTCGGGTA+GGG - chr1.3:801397-801416 None:intergenic 55.0%
! CCCCCAACTTGAGGTACTAC+CGG - chr1.3:802500-802519 None:intergenic 55.0%
! TGATGCAGGACGAAAGGCAC+TGG - chr1.3:801745-801764 None:intergenic 55.0%
!! ATCGATTTTGGGGGCCAAGG+TGG + chr1.3:802226-802245 MS.gene64028:CDS 55.0%
!! CCGGTAGTACCTCAAGTTGG+GGG + chr1.3:802497-802516 MS.gene64028:CDS 55.0%
!! TCTTTTTCGCCAGCCGCGTA+TGG + chr1.3:802431-802450 MS.gene64028:CDS 55.0%
CACAACCCCTTCTCGAGTGC+AGG + chr1.3:799826-799845 MS.gene64028:CDS 60.0%
CCCTTCTCGAGTGCAGGAGT+TGG + chr1.3:799832-799851 MS.gene64028:CDS 60.0%
CCTCGGGTAGGGAACATGCA+AGG - chr1.3:801386-801405 None:intergenic 60.0%
CCTTGCATGTTCCCTACCCG+AGG + chr1.3:801383-801402 MS.gene64028:CDS 60.0%
CTCCGCTGAAGCATGTCCTG+AGG + chr1.3:801425-801444 MS.gene64028:CDS 60.0%
GAAGGTGGAACGAGGCAAGC+TGG + chr1.3:801330-801349 MS.gene64028:CDS 60.0%
GCCACCAACCCCTGAACTTG+AGG - chr1.3:802741-802760 None:intergenic 60.0%
GCTGGCAGCGTGGAATCTGT+TGG + chr1.3:801348-801367 MS.gene64028:CDS 60.0%
GGCACTATTGGTGCAGGCAC+AGG - chr1.3:800763-800782 None:intergenic 60.0%
GGCTGGGGGTGAAGATGAAC+TGG + chr1.3:801863-801882 MS.gene64028:CDS 60.0%
GTCTGGATGACACCTCGGGT+AGG - chr1.3:801398-801417 None:intergenic 60.0%
TTGGGGGCCAAGGTGGAAGT+CGG + chr1.3:802233-802252 MS.gene64028:CDS 60.0%
! AGCTGGTGGCGTTTCAGGGA+TGG + chr1.3:802340-802359 MS.gene64028:CDS 60.0%
! CAGGAGCTGGTGGCGTTTCA+GGG + chr1.3:802336-802355 MS.gene64028:CDS 60.0%
! GCTGGTGGCGTTTCAGGGAT+GGG + chr1.3:802341-802360 MS.gene64028:CDS 60.0%
! GGTGGACTCAAGAGGAGCTC+CGG + chr1.3:802478-802497 MS.gene64028:CDS 60.0%
!! ACGAAAGGCACTGGTGGCAG+CGG - chr1.3:801736-801755 None:intergenic 60.0%
!! TGCAGGACGAAAGGCACTGG+TGG - chr1.3:801742-801761 None:intergenic 60.0%
AACGAGGCAAGCTGGCAGCG+TGG + chr1.3:801338-801357 MS.gene64028:CDS 65.0%
CCACCTGATCCATACGCGGC+TGG - chr1.3:802443-802462 None:intergenic 65.0%
CCAGCCGCGTATGGATCAGG+TGG + chr1.3:802440-802459 MS.gene64028:CDS 65.0%
CCTGAAACGCCACCAGCTCC+TGG - chr1.3:802338-802357 None:intergenic 65.0%
CGTTTCAGGGATGGGCCCTG+CGG + chr1.3:802349-802368 MS.gene64028:CDS 65.0%
TCGCCAGCCGCGTATGGATC+AGG + chr1.3:802437-802456 MS.gene64028:CDS 65.0%
! CCAGGAGCTGGTGGCGTTTC+AGG + chr1.3:802335-802354 MS.gene64028:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 799779 802841 799779 ID=MS.gene64028
chr1.3 mRNA 799779 802841 799779 ID=MS.gene64028.t1;Parent=MS.gene64028
chr1.3 exon 799779 802841 799779 ID=MS.gene64028.t1.exon1;Parent=MS.gene64028.t1
chr1.3 CDS 799779 802841 799779 ID=cds.MS.gene64028.t1;Parent=MS.gene64028.t1
Gene Sequence

>MS.gene64028

ATGACTCTTGAAGACTTCTTTACTTTGACTGAGATGAAAGATGGGCTCACAACCCCTTCTCGAGTGCAGGAGTTGGTCTCTGTGATGAAGAAGGAGCAAGACAGTATTGTGAAGAACACTGGCGATGCAATAAGACAATGGGCTGCTGTTGCAAGCACTATTGCTGCTACAGAGAATAAAGATTGCCTTGACCTTTTTATTCAATTAGAAGGACCGTGGTTCATTGATAGATGGTTAAATGATGCTCAAAAATTGGGTGGTGGTACAAATGATAGTGTCATGGAAGAGTCAATTACTGCAATGTTACGAGCAGTTGAAAAGCTATATCAAGACAGTGAGAAGCTAATTTCATCAGGAATGTGGGCCACTGTAAGTAACCTTCTTGGTCATCACAGCTCCAAGGTTCAAGATAGAGCAAGAGCACTATTTGACAAGTGGAAAGAAGTTGGAAATGGAGATGCTAAGTCTCATGATATGGATACTGGTCAAAGAAACCATATGATTGATAAGAATCTCAAGGAGGAAGGCCAGTTGTCTTCAGTGAACGGGGCTAGTAATGACAATGTTCATGTGTTGCGACTTGAAGGGGGTGAGAAGTCTATACTGAGAAGCTCAGATACTCAGATACCAGATAAGGCTGCTAATGTTAAGAAAGAGAGTTCTGACAATGCACATCAGTCCTCTGCAAGTTTGAATTGTGAAGAGCTCAAAGAGAGATCAAACCATCTTACTACTGTTTTGACTTCTGTTCAAGAAAATGTTTCCTCAAGTGAAAGTGAATTGACATCGTCTGGGATCTGCAACTTACCTGTACCGAAACAAGGAAGTTTCAAAGATCAGCCTGATGATTTACAGTTAAAAGACTTATCAATGAAGGAGGAGCAGGAACTAAATGATAAAGGCCCTCCAGAGAAATTAGGAGCACCAATAAATCCTAAGCCTGAATCTGTTTCTGTTGGTGCTAGTGAAGCACAAGTTAAGCCTGTGCCTGCACCAATAGTGCCTGAATCTTCTTTGGAACATGATGTCAAAAGCAGTGAAGTTGGTATTTGTGATAAAGTAATAGTTTCTGGTAGCATGAAAACACCTGCCTCTGATAAAATGAGTGTGGTGGATGGTGCCAGAGCCACAGATTCTAGCAATCCACAACTATCTAAAGCTTCAGTGGAGGAAGAAGGCAACAGCCAAGTTTCTAATCACGGCGACGATACCTCAAATGGCTCTGATTCTTTTAAACAAAGAAAAGATCCGACAAGCCCCAACATTATTGACAAGAGTTCTGACATGGAACTTGATTATGGTATTGTTGATGCTTTAGATGTTCCTCGACAAGTTGCTGAGGAAGTTACTCAAGTTTCTGATCAAGATAATGATACCTCAAATAGTTCTGATTCCTTCAAACAGAGCAAAGTTTCCAGAAGCGCTAACATTGTGACCAAGAATTCTGAAATTGAACTTGACTATGGCATGGTTGATGCTTTACAAGTCGCTAGACAAGTTGCTGAGGAAGTTGAGAGAGAAATCAATAACTCTTCTTCAGAGAAAAGCTCAGAAGGTGGAACGAGGCAAGCTGGCAGCGTGGAATCTGTTGGTAAAAATGATGACCTTGCATGTTCCCTACCCGAGGTGTCATCCAGACAAAGCAACTCCGCTGAAGCATGTCCTGAGGAAAGGCATATGAATGTTTCAGATGATGTTGTAGCTGAGCCAGAATGCATCCCTGACTTGGAGTCTTCGCAGTTGACTGAAGCAGCTCAAGATCCAGGAGGTAATTCAGAGAAAAGCCTTTGCACCTTTGATCTTAATGAAGAATATGGTTCTGATGATATGAATGTTTCTGCAAATACCATATCTACAACACCGATACCTGTTGTCTCTGCTTCAAAACCTGCACAGACTTCAGGATTGCCAACAGCCCCTCTACAGTTTGAAGGAACGCTTGGATGGAAAGGGTCCGCTGCCACCAGTGCCTTTCGTCCTGCATCACCTCGTAAAAATGCTGATAATCAGAAGAATGTTTCTGCTGGTGGGAACTCTGATATATCCAAACAAAGGCAAGATTTTCTTGATTTTGATCTGAATGTGGCTGGGGGTGAAGATGAACTGGCAAAACAAATTGGTGAATCTTCAGGCTTGCCTTCTGGGCAATCATCAGTGGAACATAGCCCTAAAAGATCAAAGAGATTTGAATTAGATTTGAATAGTATTGGTGATGATGGTGATACCCAACCTTCAGATCAGAGGATGGAAGGGCAGCTCTTCTTTGGAAGAAATGGCTATTGGAGCCCATCACCTGCATCATCATCGTCGTCAATGCAGCCGTCAGTTCGAAACATTGATTTGAATGATAGACCGTACTTTCAAACTGATTTACTGGACCAAGGGCCTACCAAATCTTCTTCAAGTATTGAAGTATATGGACTTTCCAAATCAGATGCTCCTGCAATATCGATTTTGGGGGCCAAGGTGGAAGTCGGTAGAAAAGAGCATGTTCCTCAAATTTGGTCTTTGCCAAATGGCAAGGCTGTTGAGCCTGCAGTAGATCTTACAATGATGCCAGGAGCTGGTGGCGTTTCAGGGATGGGCCCTGCGGTATCCTACAATCATTCAACTTTTTTGGGATATAATGGACTGACATCAATGCCCCCTTTGTCTTTTTCGCCAGCCGCGTATGGATCAGGTGGTACAGTTCCTTACATGGTGGACTCAAGAGGAGCTCCGGTAGTACCTCAAGTTGGGGGATCTTCCTCAAATGTCCTATCATCTTACGCTCAGCCCCCATATATTATGAGCATGGCTGGTACACAGCTTGGTTTAAACGGTGTTGGACCTTCCCGTCCCAACTTTGATCTTAACTCGGGTTTCATGATTGATGGCGGGAATAGAGATGCATTGACTGCAAGGCCGTTTTTCTTTCCTGGTCAGAACAGAGCTATGGAAGATAGGACCCTTCAACAATCCTCAAGTTCAGGGGTTGGTGGCAAAAGAAAAGAACCAGATGGTAGCGGTTGGGAAACATATCCATTTGGCTACAAACATCAGCAACAACCTCCGTGGAAGTAG
Protein sequence

>MS.gene64028.t1

MTLEDFFTLTEMKDGLTTPSRVQELVSVMKKEQDSIVKNTGDAIRQWAAVASTIAATENKDCLDLFIQLEGPWFIDRWLNDAQKLGGGTNDSVMEESITAMLRAVEKLYQDSEKLISSGMWATVSNLLGHHSSKVQDRARALFDKWKEVGNGDAKSHDMDTGQRNHMIDKNLKEEGQLSSVNGASNDNVHVLRLEGGEKSILRSSDTQIPDKAANVKKESSDNAHQSSASLNCEELKERSNHLTTVLTSVQENVSSSESELTSSGICNLPVPKQGSFKDQPDDLQLKDLSMKEEQELNDKGPPEKLGAPINPKPESVSVGASEAQVKPVPAPIVPESSLEHDVKSSEVGICDKVIVSGSMKTPASDKMSVVDGARATDSSNPQLSKASVEEEGNSQVSNHGDDTSNGSDSFKQRKDPTSPNIIDKSSDMELDYGIVDALDVPRQVAEEVTQVSDQDNDTSNSSDSFKQSKVSRSANIVTKNSEIELDYGMVDALQVARQVAEEVEREINNSSSEKSSEGGTRQAGSVESVGKNDDLACSLPEVSSRQSNSAEACPEERHMNVSDDVVAEPECIPDLESSQLTEAAQDPGGNSEKSLCTFDLNEEYGSDDMNVSANTISTTPIPVVSASKPAQTSGLPTAPLQFEGTLGWKGSAATSAFRPASPRKNADNQKNVSAGGNSDISKQRQDFLDFDLNVAGGEDELAKQIGESSGLPSGQSSVEHSPKRSKRFELDLNSIGDDGDTQPSDQRMEGQLFFGRNGYWSPSPASSSSSMQPSVRNIDLNDRPYFQTDLLDQGPTKSSSSIEVYGLSKSDAPAISILGAKVEVGRKEHVPQIWSLPNGKAVEPAVDLTMMPGAGGVSGMGPAVSYNHSTFLGYNGLTSMPPLSFSPAAYGSGGTVPYMVDSRGAPVVPQVGGSSSNVLSSYAQPPYIMSMAGTQLGLNGVGPSRPNFDLNSGFMIDGGNRDALTARPFFFPGQNRAMEDRTLQQSSSSGVGGKRKEPDGSGWETYPFGYKHQQQPPWK