AlfalfaGEDB | MS.gene64257

M. sativa cultivar XinJiangDaYe / MS.gene64257

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64257.t1	XP_013467138.1	82	362	26	3	1	361	1	324	9.40E-130	473.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64257.t1	Q9M8K2	30.5	105	61	3	259	359	228	324	3.3e-06	54.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64257.t1	A0A072VGJ6	82.0	362	26	3	1	361	1	324	6.8e-130	473.4

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene64257.t1	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64257.t1	MTR_1g046340	60.111	361	113	5	2	361	5	335	9.23e-133	382
MS.gene64257.t1	MTR_1g046200	92.308	169	12	1	194	361	156	324	1.07e-111	328
MS.gene64257.t1	MTR_1g046320	72.941	170	44	1	191	360	166	333	1.02e-85	263
MS.gene64257.t1	MTR_3g088515	46.718	259	107	6	2	256	16	247	1.72e-51	172

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64257.t1	AT3G06160	22.817	355	220	7	6	359	23	324	5.25e-16	78.6
MS.gene64257.t1	AT3G06160	22.817	355	220	7	6	359	8	309	5.87e-16	78.2
MS.gene64257.t1	AT3G06160	22.817	355	220	7	6	359	8	309	5.87e-16	78.2
MS.gene64257.t1	AT3G06160	22.817	355	220	7	6	359	8	309	5.87e-16	78.2
MS.gene64257.t1	AT3G06160	23.380	355	218	8	6	359	67	368	8.16e-16	78.2

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Find 62 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAGAAGGTAAATGACG+AGG	0.671120	1.3:-27776605	MS.gene64257:CDS
AAAGAATTGTACCAATTCAT+TGG	0.326483	1.3:+27776074	None:intergenic
AAATCCTTGATAACATTCTT+TGG	0.259575	1.3:+27775730	None:intergenic
AAGAATTGTACCAATTCATT+GGG	0.434349	1.3:+27776075	None:intergenic
AAGGGTGCACATTGTTAGGA+AGG	0.552584	1.3:-27775331	MS.gene64257:CDS
AATTGTAACTAAGATGGCCA+AGG	0.605912	1.3:-27777161	None:intergenic
ACAACACATATCGAAAAGAG+AGG	0.662380	1.3:-27775489	MS.gene64257:CDS
AGAACCTGCAAGTGCAAAGC+TGG	0.504053	1.3:-27776319	MS.gene64257:intron
AGAGAAGCACGCTGAAAGAA+TGG	0.532134	1.3:-27777107	MS.gene64257:intron
AGAGAGGCCAAGTACGTATA+TGG	0.345367	1.3:-27775473	MS.gene64257:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAACAATTGGAGGAAGAA+TGG	+	chr1.3:27775414-27775433	None:intergenic	30.0%
AAACTGAAATTGCAACAACT+TGG	+	chr1.3:27776648-27776667	None:intergenic	30.0%
AATAAAGAGAATAACCAGAC+AGG	+	chr1.3:27776563-27776582	None:intergenic	30.0%
AATACACTTATTAGACTCTG+AGG	+	chr1.3:27775507-27775526	None:intergenic	30.0%
ATCAATGTGTGAAAAGAAGA+AGG	-	chr1.3:27775857-27775876	MS.gene64257:intron	30.0%
ATGGAACAATCGAAATGTTA+AGG	-	chr1.3:27777124-27777143	MS.gene64257:CDS	30.0%
CGAGAATGAAAGAATGTATA+AGG	-	chr1.3:27776103-27776122	MS.gene64257:CDS	30.0%
TATATCTTTGCATACCAAAC+TGG	+	chr1.3:27775461-27775480	None:intergenic	30.0%
TGCTGAAATGTATATTCAAC+CGG	-	chr1.3:27776338-27776357	MS.gene64257:CDS	30.0%
TGGAACAATCGAAATGTTAA+GGG	-	chr1.3:27777125-27777144	MS.gene64257:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	27775326	27777170	27775326	ID=MS.gene64257
chr1.3	mRNA	27775326	27777170	27775326	ID=MS.gene64257.t1;Parent=MS.gene64257
chr1.3	exon	27777108	27777170	27777108	ID=MS.gene64257.t1.exon1;Parent=MS.gene64257.t1
chr1.3	CDS	27777108	27777170	27777108	ID=cds.MS.gene64257.t1;Parent=MS.gene64257.t1
chr1.3	exon	27776320	27776899	27776320	ID=MS.gene64257.t1.exon2;Parent=MS.gene64257.t1
chr1.3	CDS	27776320	27776899	27776320	ID=cds.MS.gene64257.t1;Parent=MS.gene64257.t1
chr1.3	exon	27776086	27776210	27776086	ID=MS.gene64257.t1.exon3;Parent=MS.gene64257.t1
chr1.3	CDS	27776086	27776210	27776086	ID=cds.MS.gene64257.t1;Parent=MS.gene64257.t1
chr1.3	exon	27775681	27775758	27775681	ID=MS.gene64257.t1.exon4;Parent=MS.gene64257.t1
chr1.3	CDS	27775681	27775758	27775681	ID=cds.MS.gene64257.t1;Parent=MS.gene64257.t1
chr1.3	exon	27775326	27775565	27775326	ID=MS.gene64257.t1.exon5;Parent=MS.gene64257.t1
chr1.3	CDS	27775326	27775565	27775326	ID=cds.MS.gene64257.t1;Parent=MS.gene64257.t1

Gene Sequence

>MS.gene64257

ATGGCCAAGGATTACCCAGACTTCTTCAAAGTTTTTTCCTCAGAGAAGCACGCTGAAAGAATGGTACGTACTTGTTTATTCTTAACCATTCTTCCTCCAATTGTTTTTTTTTCTATTTTTTTGATAAAAAAACCAGTTTGGTATGCAAAGATATATCAAATCCCAAGTAATGTTATCTCCTCAGAGTCTAATAAGTGTATTGAAAATAACTTGTGATGTAGTAGCTATTTACTACTAATTAATCTTCATCATTTTCTTTAATCTATTGCAGCGTATCCCTAATGCTTTTGTGAACTTGATGCGTTCAAAAAGGAACTTGATGCGTTCAAAAAGGAGGGTAATAAATGATTTTATCCTAAGAGATCGTAGACGAAGAGATTGGCATGTCAAAGCTCGTCGTATTGGCGATGAGCTTTATTTTGACGATGGCTGGAAGCAATCTAGAGATGAAAATTCTTTAGAGGAGGACGATTTCCTTGTTTTTACACATATTGAAAACAACGTATTCAGGTTCAAAATTCTTCAACTATCATCAATGTGTGAAAAGAAGAAGGTAAATGACGAGGAGGAAAATAATAATATGATGGAGGATGAGGAAGAGGACAATGATGGTGATGATGATGACGACGATGATGAGGATGATGATTATGATTATGATGGTGATGATGCTGCTGCTGCTGCTGTTGATGATATTATGGCAGAGGAAGAAGATCATGATGATGATATTACGGCAGAGGAAGAACATCATGATACCGATGATGATGGTGATGACAACGACGAGAATGAAAGAATGTATAAGGAAATTAGTAGAAGTAAACATCAACATTGTAGAACCTGCAAGTGCAAAGCTGGTAAGTTTTGACATCATTCTGAAGATAAAACTACTATTTTGTATTTTCATTTTATATGAGTTTGTGCATGCATGAGTCAGAATTTACCTGATGATTTATTTTGGTTTAGCGGACATTGGGAGCAGCTCTGCACGTTCAAAACTCGAAGATAACGAAATTGATGCTGAAATGTATATTCAACCGGGAAATCCACACTTTATTGCAAAATATATTCATTCCAGACCCAATGAATTGGTACAATTCTTTTTGTCCATTACTTTTTTTGACATGGAAGCATAGTTAAATGAGTGATTAGTCCATTTGCTCAACCCTAGGTAGAGGAGTTAGTCTTAATTTTCACCTTTTACTTTTCATTTCTTAACAATTTTCCTTTGTACTTTATGCCTGTCTGGTTATTCTCTTTATTATTTTCATGTCCATATTGAAAATGTTGATTGAGTGATTCTCTATTTTATGACTCCAAACAAGCCAAGTTGTTGCAATTTCAGTTTTCATAATTTACAATCATTTCATGTTACTTCTTTGATTTACTTATCATGCCATCACTCTTCACATTTGAAGTTTATTCCAAAGAATGTTATCAAGGATTTCTGCCTCTACTTCACAAAACATGTCACCCTTGTGTGTTGCCACTGTAAGGTATAATTCTAATCTTTACCTTTCATTTTTCACAATTATGAACTGATGTTACTTTTGTTTAATGTGTCAATAATTTGATATTGAAGTTGAATTCATCCTTTTTTGGTTATGACAGGACATTCAGAGTAATGACTTAGCGGCCTATCATCACATCTTGCCACAAATAAGTACAACACATATCGAAAAGAGAGGCCAAGTACGTATATGGAGAGATGGTCGAGTACGTGTGCTAGGGTGGGAAGATTTTTGTGGAAAGAGCAAGATCACAGAAAATGATAGTTGCTTATGCGAGATTGTGCTGCGTGAAGATGGAACAATCGAAATGTTAAGGGTGCACATTGTTAGGAAGGAATGA

Protein sequence

>MS.gene64257.t1

MAKDYPDFFKVFSSEKHAERMRIPNAFVNLMRSKRNLMRSKRRVINDFILRDRRRRDWHVKARRIGDELYFDDGWKQSRDENSLEEDDFLVFTHIENNVFRFKILQLSSMCEKKKVNDEEENNNMMEDEEEDNDGDDDDDDDEDDDYDYDGDDAAAAAVDDIMAEEEDHDDDITAEEEHHDTDDDGDDNDENERMYKEISRSKHQHCRTCKCKAADIGSSSARSKLEDNEIDAEMYIQPGNPHFIAKYIHSRPNELFIPKNVIKDFCLYFTKHVTLVCCHCKDIQSNDLAAYHHILPQISTTHIEKRGQVRIWRDGRVRVLGWEDFCGKSKITENDSCLCEIVLREDGTIEMLRVHIVRKE