AlfalfaGEDB | MS.gene64371

M. sativa cultivar XinJiangDaYe / MS.gene64371

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64371.t1	AES63738.2	84.2	120	17	1	1	118	1	120	1.70E-51	211.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64371.t1	G7INX6	84.2	120	17	1	1	118	1	120	1.2e-51	211.8

TFs/TRs:

Gene ID	Type	Classification
MS.gene64371.t1	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64371.t1	MTR_2g012700	84.167	120	17	1	1	118	1	120	2.87e-70	206
MS.gene64371.t1	MTR_2g062330	79.661	118	21	2	3	118	23	139	6.13e-63	188
MS.gene64371.t1	MTR_7g117590	36.154	130	62	5	7	117	44	171	3.09e-19	78.6
MS.gene64371.t1	MTR_7g117585	33.663	101	63	1	18	114	42	142	2.09e-15	67.8
MS.gene64371.t1	MTR_5g071410	38.961	77	47	0	38	114	189	265	3.46e-13	64.3
MS.gene64371.t1	MTR_3g008190	27.835	97	68	1	24	118	6	102	1.80e-12	59.3
MS.gene64371.t1	MTR_0010s0310	38.462	91	52	2	22	108	150	240	2.43e-12	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 20 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAATGTTACTGCTTCTATTT+TGG	0.123735	2.4:+36180757	MS.gene64371:CDS
GAAGTTAATGGTGCTGAATT+AGG	0.163350	2.4:+36180698	MS.gene64371:CDS
AAAATTCCGATGTTCATTAT+TGG	0.199581	2.4:+36180729	MS.gene64371:CDS
GAGATTGGAGTTGAAGTTAA+TGG	0.344431	2.4:+36180686	MS.gene64371:CDS
AAGTTAATGGTGCTGAATTA+GGG	0.367629	2.4:+36180699	MS.gene64371:CDS
CAGACAATAATCCTCCTAAT+AGG	0.388317	2.4:+36181094	MS.gene64371:CDS
TTACCAGTTTCTTCATCAAA+AGG	0.404700	2.4:-36180952	None:intergenic
CCTCGACGTATTGCTGAGAA+TGG	0.434646	2.4:+36180901	MS.gene64371:CDS
ACGGTAGCATACATCCTATT+AGG	0.440754	2.4:-36181108	None:intergenic
GTATGCTACCGTCGTTAACT+AGG	0.487453	2.4:+36181119	MS.gene64371:CDS
TACCAGTTTCTTCATCAAAA+GGG	0.488019	2.4:-36180951	None:intergenic
AGCATGCATCAGAAATACAT+AGG	0.564126	2.4:+36181009	MS.gene64371:CDS
CTTGAAAAGTGCCTAAAAGT+TGG	0.570138	2.4:+36181054	MS.gene64371:CDS
AGAAATACATAGGGAGAGGA+TGG	0.573439	2.4:+36181019	MS.gene64371:CDS
CATCAGAAATACATAGGGAG+AGG	0.580360	2.4:+36181015	MS.gene64371:CDS
GCATGCATCAGAAATACATA+GGG	0.596673	2.4:+36181010	MS.gene64371:CDS
GTAGCATACATCCTATTAGG+AGG	0.633251	2.4:-36181105	None:intergenic
ATACTTCCAATAATGAACAT+CGG	0.646998	2.4:-36180735	None:intergenic
CCATTCTCAGCAATACGTCG+AGG	0.710417	2.4:-36180901	None:intergenic
GAATCAGTCCTAGTTAACGA+CGG	0.726011	2.4:-36181127	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAATTCCGATGTTCATTAT+TGG	+	chr2.4:36180729-36180748	MS.gene64371:CDS	25.0%
!	ATACTTCCAATAATGAACAT+CGG	-	chr2.4:36180738-36180757	None:intergenic	25.0%
!!	TAATGTTACTGCTTCTATTT+TGG	+	chr2.4:36180757-36180776	MS.gene64371:CDS	25.0%
	TACCAGTTTCTTCATCAAAA+GGG	-	chr2.4:36180954-36180973	None:intergenic	30.0%
	TTACCAGTTTCTTCATCAAA+AGG	-	chr2.4:36180955-36180974	None:intergenic	30.0%
!	AAATAACGTCACCAACTTTT+AGG	-	chr2.4:36181068-36181087	None:intergenic	30.0%
!	AAGTTAATGGTGCTGAATTA+GGG	+	chr2.4:36180699-36180718	MS.gene64371:CDS	30.0%
!	TTTTGGATGGAAACAATGTT+TGG	+	chr2.4:36180774-36180793	MS.gene64371:intron	30.0%
	AGCATGCATCAGAAATACAT+AGG	+	chr2.4:36181009-36181028	MS.gene64371:CDS	35.0%
	CAGACAATAATCCTCCTAAT+AGG	+	chr2.4:36181094-36181113	MS.gene64371:CDS	35.0%
	GAGATTGGAGTTGAAGTTAA+TGG	+	chr2.4:36180686-36180705	MS.gene64371:CDS	35.0%
	GCATGCATCAGAAATACATA+GGG	+	chr2.4:36181010-36181029	MS.gene64371:CDS	35.0%
	TGGAAACAATGTTTGGTTAG+TGG	+	chr2.4:36180781-36180800	MS.gene64371:intron	35.0%
!	GAAGTTAATGGTGCTGAATT+AGG	+	chr2.4:36180698-36180717	MS.gene64371:CDS	35.0%
!!	CTTGAAAAGTGCCTAAAAGT+TGG	+	chr2.4:36181054-36181073	MS.gene64371:CDS	35.0%
!!	GTTACTGCTTCTATTTTGGA+TGG	+	chr2.4:36180761-36180780	MS.gene64371:CDS	35.0%
	ACGGTAGCATACATCCTATT+AGG	-	chr2.4:36181111-36181130	None:intergenic	40.0%
	AGAAATACATAGGGAGAGGA+TGG	+	chr2.4:36181019-36181038	MS.gene64371:CDS	40.0%
	CATCAGAAATACATAGGGAG+AGG	+	chr2.4:36181015-36181034	MS.gene64371:CDS	40.0%
	GTAGCATACATCCTATTAGG+AGG	-	chr2.4:36181108-36181127	None:intergenic	40.0%
!	CTCCCTTTTGATGAAGAAAC+TGG	+	chr2.4:36180949-36180968	MS.gene64371:CDS	40.0%
	CCATTCTCAGCAATACGTCG+AGG	-	chr2.4:36180904-36180923	None:intergenic	50.0%
	CCTCGACGTATTGCTGAGAA+TGG	+	chr2.4:36180901-36180920	MS.gene64371:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	36180680	36181140	36180680	ID=MS.gene64371
chr2.4	mRNA	36180680	36181140	36180680	ID=MS.gene64371.t1;Parent=MS.gene64371
chr2.4	exon	36180680	36180790	36180680	ID=MS.gene64371.t1.exon1;Parent=MS.gene64371.t1
chr2.4	CDS	36180680	36180790	36180680	ID=cds.MS.gene64371.t1;Parent=MS.gene64371.t1
chr2.4	exon	36180895	36181140	36180895	ID=MS.gene64371.t1.exon2;Parent=MS.gene64371.t1
chr2.4	CDS	36180895	36181140	36180895	ID=cds.MS.gene64371.t1;Parent=MS.gene64371.t1

Gene Sequence

>MS.gene64371

ATGAATGAGATTGGAGTTGAAGTTAATGGTGCTGAATTAGGGAATCTTCAAAATTCCGATGTTCATTATTGGAAGTATAATGTTACTGCTTCTATTTTGGATGGAAACAATGTTTGGTTAGTGGCATTTACGAAACATTTTTTTTTTGTTTTCATGTTGTTAAGTTGTTGTTGATTAATTATAATTTGTATGATTTCATGACAAATCTATTGTAGAATTTTCCTCGACGTATTGCTGAGAATGGTTTGTATATCAATCAATCTAAAATTCTCCCTTTTGATGAAGAAACTGGTAAATCTTATGACTGTGATGTTCACACTGCGTCAAGAAGCATGCATCAGAAATACATAGGGAGAGGATGGTACGACTTTGTTCTTGAAAAGTGCCTAAAAGTTGGTGACGTTATTTTGTTCACAGACAATAATCCTCCTAATAGGATGTATGCTACCGTCGTTAACTAG

Protein sequence

>MS.gene64371.t1

MNEIGVEVNGAELGNLQNSDVHYWKYNVTASILDGNNNFPRRIAENGLYINQSKILPFDEETGKSYDCDVHTASRSMHQKYIGRGWYDFVLEKCLKVGDVILFTDNNPPNRMYATVVN