AlfalfaGEDB | MS.gene64421

M. sativa cultivar XinJiangDaYe / MS.gene64421

KEGG: -
GO: GO:0000166 GO:0003674 GO:0005488 GO:0005524 GO:0005575 GO:0005634 GO:0006355 GO:0008144 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0017076 GO:0019219 GO:0019222 GO:0030554 GO:0031323 GO:0031326 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0097367 GO:0110165 GO:1901265 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF08879.13 PF08880.14

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64421.t1	XP_024633316.1	88.8	366	31	3	1	365	1	357	2.00E-167	598.6

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64421.t1	Q8L8A6	73.3	101	23	2	7	107	16	112	1.4e-39	165.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64421.t1	A0A072V671	88.8	366	31	3	1	365	1	357	1.5e-167	598.6

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene64421.t1	TF	GRF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64421.t1	MTR_2g041430	94.536	366	10	4	1	365	1	357	0.0	698
MS.gene64421.t1	MTR_2g041430	94.490	363	10	4	1	362	1	354	0.0	692
MS.gene64421.t1	MTR_2g041430	94.490	363	10	4	1	362	1	354	0.0	692
MS.gene64421.t1	MTR_4g125490	64.042	381	93	14	3	360	5	364	6.13e-144	415
MS.gene64421.t1	MTR_8g020560	45.559	349	155	13	3	333	7	338	2.84e-69	221
MS.gene64421.t1	MTR_0001s0490	39.714	350	148	13	7	347	25	320	2.12e-58	193
MS.gene64421.t1	MTR_1g017490	57.983	119	42	2	5	119	9	123	1.12e-40	150
MS.gene64421.t1	MTR_1g017490	57.983	119	42	2	5	119	142	256	1.89e-40	151
MS.gene64421.t1	MTR_5g027030	60.177	113	29	2	7	119	16	112	3.76e-40	145
MS.gene64421.t1	MTR_7g104050	54.198	131	45	4	4	125	68	192	5.48e-35	132

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64421.t1	AT3G13960	71.681	113	28	2	7	119	16	124	3.62e-55	186
MS.gene64421.t1	AT2G06200	64.167	120	36	2	5	117	4	123	4.71e-47	160
MS.gene64421.t1	AT2G06200	64.167	120	36	2	5	117	43	162	5.60e-47	162
MS.gene64421.t1	AT4G37740	58.120	117	41	4	5	120	162	271	1.15e-38	145
MS.gene64421.t1	AT2G36400	56.198	121	50	2	8	127	77	195	1.10e-36	137
MS.gene64421.t1	AT2G22840	56.897	116	42	4	5	119	131	239	7.24e-34	132
MS.gene64421.t1	AT3G52910	55.172	116	44	4	8	119	83	194	5.38e-33	127
MS.gene64421.t1	AT2G06200	92.157	51	2	1	69	117	1	51	3.26e-26	103
MS.gene64421.t1	AT5G53660	48.246	114	38	2	6	119	58	150	1.62e-25	106
MS.gene64421.t1	AT4G24150	44.444	117	55	2	8	119	112	223	1.17e-23	102

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Find 55 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGAACAGGTATGTGTA+TGG	0.489899	2.4:+48678266	MS.gene64421:intron
AAACCGTTCAAGAAAGCCTG+TGG	0.641388	2.4:+48677279	MS.gene64421:CDS
AACTATCTGCAAATGGGTTT+AGG	0.375973	2.4:+48677139	MS.gene64421:CDS
AAGAATTAGTTAATGATGCA+GGG	0.570085	2.4:-48677387	None:intergenic
AAGCTTCTCTGCTTCATCAA+TGG	0.443492	2.4:+48678352	MS.gene64421:CDS
AATCGAAATCGATAATTGAG+TGG	0.635985	2.4:-48678618	None:intergenic
AGAAAGATAGATCCAGAGCC+AGG	0.511253	2.4:+48677163	MS.gene64421:CDS
AGAATTGCTATCAAGAAACA+AGG	0.601977	2.4:-48677566	None:intergenic
AGAGAAAGGACTTACAGTGT+TGG	0.638023	2.4:-48676482	None:intergenic
AGCTCTTATTTACAAGTACA+TGG	0.521532	2.4:+48676362	MS.gene64421:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATCGAAATCGATAATTGAG+TGG	-	chr2.4:48678621-48678640	None:intergenic	30.0%
AGAATTGCTATCAAGAAACA+AGG	-	chr2.4:48677569-48677588	None:intergenic	30.0%
AGTTGTATGAGGATACATAA+AGG	-	chr2.4:48677491-48677510	None:intergenic	30.0%
CACACATTAAGAGATCAAAA+TGG	+	chr2.4:48678116-48678135	MS.gene64421:intron	30.0%
CATAAAGGATTGTTGCAAAT+TGG	-	chr2.4:48677476-48677495	None:intergenic	30.0%
CATAGAGATTCATATCCATT+CGG	+	chr2.4:48678703-48678722	MS.gene64421:intron	30.0%
CTATGAAAGTTCAATTACCA+TGG	-	chr2.4:48678688-48678707	None:intergenic	30.0%
GAAACAAAGTTCAAACACTA+TGG	-	chr2.4:48677631-48677650	None:intergenic	30.0%
GAAGAATTAGTTAATGATGC+AGG	-	chr2.4:48677391-48677410	None:intergenic	30.0%
GAATTGCTATCAAGAAACAA+GGG	-	chr2.4:48677568-48677587	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	48676306	48678825	48676306	ID=MS.gene64421
chr2.4	mRNA	48676306	48678825	48676306	ID=MS.gene64421.t1;Parent=MS.gene64421
chr2.4	exon	48676306	48676489	48676306	ID=MS.gene64421.t1.exon1;Parent=MS.gene64421.t1
chr2.4	CDS	48676306	48676489	48676306	ID=cds.MS.gene64421.t1;Parent=MS.gene64421.t1
chr2.4	exon	48677131	48677614	48677131	ID=MS.gene64421.t1.exon2;Parent=MS.gene64421.t1
chr2.4	CDS	48677131	48677614	48677131	ID=cds.MS.gene64421.t1;Parent=MS.gene64421.t1
chr2.4	exon	48678271	48678689	48678271	ID=MS.gene64421.t1.exon3;Parent=MS.gene64421.t1
chr2.4	CDS	48678271	48678689	48678271	ID=cds.MS.gene64421.t1;Parent=MS.gene64421.t1
chr2.4	exon	48678773	48678825	48678773	ID=MS.gene64421.t1.exon4;Parent=MS.gene64421.t1
chr2.4	CDS	48678773	48678825	48678773	ID=cds.MS.gene64421.t1;Parent=MS.gene64421.t1

Gene Sequence

>MS.gene64421

ATGAACAAGAACAAGTTTCCTTTTACTCCATCACAATGGCAAGAACTTGAACATCAAGCTCTTATTTACAAGTACATGGCTTCTGGTATATCTATTCCACCTGATCTTCTTTTCACCATCAGAAAAAGCTATTTGGACTCTCCTCTTTCTTCAAGACTTTTTCCTAATCAACAACACCAACACTGTAAGTCCTTTCTCTCCCTCTCTATATTCATATGTTTACTGTTAAGAGTAACATAGTTCACATCTTTATATAGACAATTAATAATAATTTTTTCCTTCTTAATTTTTTTTTAAAAAACTTTTCTAAAATGAACAGTTTAATTTAGATTATAAATAAATAAATAAAATTATTAGTAATAATAACTGTTCATAATTTGATTAATAGTTTTTTTTAGACAAATTTGGAAAGTAAGAAATTATTTTATTTATAAAAGTGATATTAATGATCATTTTACGACGATCTTTAAAAAGATTTAAACTCGAGATTAGTATCTCAAACTCGTACCACGGACCTAACACCTTTGATATAGTATACATCTTCGGATGTGTTTGGTTATCCGTTTGCAACACACAAAAGCACGTTTGCACATTTTTCGATGCGTTTTTCCGTGATTTTCAGAAGCTCCAAATACTATCTTTTTTGCTAACATGGTCTTTTGCCGTGATTTCCATCAAACACCGTTTTGCAAACGCTGTACGAAACAGACACTTCCTAAAAGATTATGAATGTATAGAGATAATTTAGTTAAAGGAGTGAAATTATTGTCTCAAGTGTCACAAAATGAGTCTCTTTGACCAAAGGGTTATTTTGATTTTTTTCAGTTGGATGGAACTATCTGCAAATGGGTTTAGGAAGAAAGATAGATCCAGAGCCAGGTAGATGTAGAAGAACAGATGGAAAGAAATGGAGATGTTCAAAAGAAGCATATCCAGATTCTAAATATTGTGAAAGACACATGCATAGAGGAAAAAACCGTTCAAGAAAGCCTGTGGAAGTTGTAAAAACAACAACAACAACAACAAATACAAATATTAATAATGCTTCATCACTTGGAATTTCATCACTTACCAAAAATAGCCCTGCATCATTAACTAATTCTTCTTCTTTGTCTTTTGAGACTCAACAACATCACCAAAACTATCCTCAAAATTCTTGCTATGGTTCCAATTTGCAACAATCCTTTATGTATCCTCATACAACTTCAAGGTCAACTTTGTCATCTGGTATTGGTTTGTCTTTTGAAGACAATAGTGCTCCCTTGTTTCTTGATAGCAATTCTTGCTCTCAGAATAACACTGATTACAGGTTCGTCTTGAAACCATAGTGTTTGAACTTTGTTTCGGTGTTTTGATAAATAGTCGTTTTTGGAGTAAATAGTCATTTTGGTATTTGAACGTGTATGTCAGTGCCATGTCATTTTTGCAGTGAAGCATAACAAAATTACAAAAACATATGAATATTTTTTTTGTTAGTTAGTTTGTTGGATGTGTCTTTTGAATTACTAACAAAGACAGGCTCGACGATATTTCTGTAATTTCGATACATTAAGAGATTATGATGACATTGTCTTACACATTTCGGTACTAAAATGATTGTTGACTCAAATTTTTTGATAAATATATGACTGCAATATGACTTCAAGTAAATAGTCATTTTGGTTTGTAGATGTGTAACTCGGAAGTTAGTTTGGTAATCGGACGTGTTACGTGTTCTCTGTAAGTCAGTTTGTCTTGAATTTAAAAATATAAGACACGTCCGTTGATTCTTTTTGTAATTTCGATACATTCAAAGAATATAATGGCACCTCACACATTAAGAGATCAAAATGGCTTCTTCAAAAAAAAAAAAAGAGATCAAAATGGCTATTTACTCACAAGATTATGATTGAGACTATGATCTTTAAATGTTTGTGAATTGTGATTGATTCTCTTTGGTTTCTGTTTTCTTCCTTTGTAAAAAAGGAACAGGTATGTGTATGGACAAAAAGAAGAGGTGGATGAGTATGCATTCTTCAAAGAACCTTCTGGTAGCAGTACTATGAAAAGCTTCTCTGCTTCATCAATGGAACATGATCCTTGGCAACTAACACCACTTACTATGAGTTCTTCCTCATCATCTTCTACTTCTTTGAGACAGAGAAGCTTTTCAGCGTTGTCGAATGATTACTCTTGCTTGCAACTTCAGAGCCTTAGTGAACACTCAAAGCAGCATGAGCAAGATGAACCTCAGAAAACTGTTCATCGCTTCTTTGATGAATGGCCACATAAAGGGAGAGATCAATGGCTTGATTTGGATGATAAATCATCTACCACTCAATTATCGATTTCGATTCCATCCTCTGGTCATGATTTTCCAACTTTCACTTCTAGAAATACCCATGGTAATTGAACTTTCATAGAGATTCATATCCATTCGGCTCAATTTCTTTGATAGTACTGTTCTAGGTTTTAAATCATGTTGCAGTTACATCACGATTCTTGATATTGCACTTACATCGTGAATTCGAACATGGTTAG

Protein sequence

>MS.gene64421.t1

MNKNKFPFTPSQWQELEHQALIYKYMASGISIPPDLLFTIRKSYLDSPLSSRLFPNQQHQHFGWNYLQMGLGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEVVKTTTTTTNTNINNASSLGISSLTKNSPASLTNSSSLSFETQQHHQNYPQNSCYGSNLQQSFMYPHTTSRSTLSSGIGLSFEDNSAPLFLDSNSCSQNNTDYRNRYVYGQKEEVDEYAFFKEPSGSSTMKSFSASSMEHDPWQLTPLTMSSSSSSSTSLRQRSFSALSNDYSCLQLQSLSEHSKQHEQDEPQKTVHRFFDEWPHKGRDQWLDLDDKSSTTQLSISIPSSGHDFPTFTSRNTHVTSRFLILHLHREFEHG